Rroxscaffold_4G00283720

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010964
Physical Location & Seq
Forward (+)
5584447 .. 5586361
1915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00283720.1

Sequence Viewer

Length: 1149 bp
ATGGAAAACACATACCCGCGGAGGCTCCAATCACATACAGCCACGTCACAACAGCACTCTTCTGGGCCTCGTCTTCTACGCAGCAGAAGCGGAAGTAGTACTTCTCCGGCGATAAACAACTCGTTATACACTCCGAGCGCCAGCCACAGATTCACCACTCCCCGGTCGAAGTCCACCGCCAAATCCAGAACCGGTTACAGAGATGAAGAGAAAACGGTAACTTCTAGCATGCAAAAGAAGGGGCAAGAGAATAGAGAAGGTAAAAATAATAGTCAGAATGCTACTTTTGCTATCCCTAGGTTGTTGCTGCAGCGCGGGAATACTAGCCCTAGTCCGAGTCCACGCCCTAGCTTTGGCTCGACGAAGGGGAGGAAGTCGCCGGTGTCTCCTTCGGCTTGGGCGTTGTCTCCGGGACGGTCTTTGCCATGCACGGTGGGACCGGAGCTGACTGCGGCGGCAGTGGCTTCGCCGAGACCGAGGGTGAAGTCTAGTGGGGGTGGAGTTGGTGGGGTTTTGAAGTATTTTAGGCTTCCGAAGAAAGGGATGACTATACAAGAGGAGGAGTTTCATCAGTTTCGGATTTTGCATAATAGGTTGCTCCAATGGAGGTTTGCCAATGCTCGAGCTCAAGCTGCCATGGTTGCTTCACACAAAGTTGCACAGGACAGAATGTTCAGTGTGTGGCTTAGAATTTTCAAAATGAGGAACTACATCCTAGATAAGCAACTGCAACTGCAAAAGCTCAAGCATCAAGTGAAGGTGTATCAAATTGTGTACCCTCAAATTTCTCTACAGAACGAATGGGGGAAATTGGAGAGAAAAAATCAAGAATCTGTGAGCAGGATGGTGAGGAAGTTATCCGGAATTTCAAATACAATTCCTTTGGTAAATGATGCTAAGGGAGATGTGGCGTCTATATACGAAGCGATGATCACAGCCATGGATGTCATGGCTGGTATTCAAGCAACGGTCACTAAATTGCTCCCGCAGCTCGAAAAGCTGCTCTACATGGTTACGGAGCTTTTGATCACGCAGAAGCAACAAGGAGAATTAGAGAAATATATAACATCGGTCGTTGGTCTTATGGTTGATATATTAGCCAAAAGAGGAGACAAGCGTGAGAGTATATCTCATCCAAGTAGCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

382

Amino Acids

42.66

Weight (kDa)

10.46

Isoelectric Point (pI)

50.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 32 - 348 7.8e-67 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 19, 315
AccIII TCCGGA 1 cut(s) 860
AciI CCGC 8 cut(s) 17, 19, 90, 177, 315, 452, 455, 986
AcsI RAATTY 3 cut(s) 690, 783, 864
AcyI GRCGYC 1 cut(s) 911
AfaI GTAC 2 cut(s) 100, 776
AfiI CCNNNNNNNGG 3 cut(s) 162, 353, 539
AgeI ACCGGT 1 cut(s) 191
AgsI TTSAA 4 cut(s) 517, 697, 870, 962
AjiI CACGTC 1 cut(s) 45
AloI GAACNNNNNNTCC 2 cut(s) 656, 688
AluBI AGCT 8 cut(s) 351, 445, 626, 632, 742, 991, 1000, 1021
AluI AGCT 8 cut(s) 351, 445, 626, 632, 742, 991, 1000, 1021
Alw21I GWGCWC 1 cut(s) 628
Alw26I GTCTC 4 cut(s) 390, 411, 466, 1104
Ama87I CYCGRG 1 cut(s) 621
Aor13HI TCCGGA 1 cut(s) 860
AoxI GGCC 1 cut(s) 65
ApeKI GCWGC 6 cut(s) 81, 307, 310, 632, 988, 1000
ApoI RAATTY 3 cut(s) 690, 783, 864
AsiGI ACCGGT 1 cut(s) 191
AspA2I CCTAGG 1 cut(s) 296
AspLEI GCGC 2 cut(s) 140, 315
AspS9I GGNCC 2 cut(s) 65, 437
AsuC2I CCSGG 2 cut(s) 163, 411
AsuHPI GGTGA 3 cut(s) 145, 493, 859
AvaI CYCGRG 1 cut(s) 621
AvaII GGWCC 1 cut(s) 437
AvrII CCTAGG 1 cut(s) 296
BanII GRGCYC 1 cut(s) 628
BbsI GAAGAC 1 cut(s) 65
Bbv12I GWGCWC 1 cut(s) 628
BbvI GCAGC 6 cut(s) 93, 294, 322, 619, 987, 1000
BccI CCATC 1 cut(s) 838
BcgI CGANNNNNNTGC 2 cut(s) 447, 481
BclI TGATCA 2 cut(s) 930, 1026
BcnI CCSGG 2 cut(s) 163, 411
BcoDI GTCTC 4 cut(s) 390, 411, 466, 1104
BfaI CTAG 7 cut(s) 225, 297, 324, 330, 348, 489, 716
BfmI CTRYAG 2 cut(s) 308, 791
BfoI RGCGCY 1 cut(s) 141
BisI GCNGC 8 cut(s) 82, 308, 311, 453, 456, 633, 989, 1001
BlnI CCTAGG 1 cut(s) 296
BlsI GCNGC 8 cut(s) 83, 309, 312, 454, 457, 634, 990, 1002
BmcAI AGTACT 1 cut(s) 100
Bme1390I CCNGG 2 cut(s) 163, 411
Bme18I GGWCC 1 cut(s) 437
BmeT110I CYCGRG 1 cut(s) 621
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 2 cut(s) 65, 437
BmiI GGNNCC 2 cut(s) 26, 438
BmrFI CCNGG 2 cut(s) 163, 411
BmsI GCATC 2 cut(s) 757, 883
BpiI GAAGAC 1 cut(s) 65
BplI GAGNNNNNCTC 2 cut(s) 1114, 1146
Bpu10I CCTNAGC 1 cut(s) 897
BpuEI CTTGAG 2 cut(s) 612, 728
BpuMI CCSGG 2 cut(s) 163, 411
BsaHI GRCGYC 1 cut(s) 911
BsaI GGTCTC 1 cut(s) 466
BsaJI CCNNGG 6 cut(s) 17, 161, 296, 476, 636, 939
BsaWI WCCGGW 3 cut(s) 191, 439, 860
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 353, 539
Bse118I RCCGGY 2 cut(s) 191, 379
BseAI TCCGGA 1 cut(s) 860
BseDI CCNNGG 6 cut(s) 17, 161, 296, 476, 636, 939
BseGI GGATG 5 cut(s) 549, 711, 849, 949, 1132
BseLI CCNNNNNNNGG 3 cut(s) 162, 353, 539
BseRI GAGGAG 3 cut(s) 572, 575, 1122
BseXI GCAGC 6 cut(s) 93, 294, 322, 619, 987, 1000
Bsh1236I CGCG 2 cut(s) 19, 315
Bsh1285I CGRYCG 2 cut(s) 167, 1074
BshFI GGCC 1 cut(s) 67
BshTI ACCGGT 1 cut(s) 191
BsiEI CGRYCG 2 cut(s) 167, 1074
BsiHKAI GWGCWC 1 cut(s) 628
BsiHKCI CYCGRG 1 cut(s) 621
BsiSI CCGG 7 cut(s) 107, 163, 192, 380, 410, 440, 861
BslFI GGGAC 2 cut(s) 426, 450
BslI CCNNNNNNNGG 3 cut(s) 162, 353, 539
BsmAI GTCTC 4 cut(s) 390, 411, 466, 1104
BsmFI GGGAC 2 cut(s) 426, 450
BsmI GAATGC 1 cut(s) 283
BsnI GGCC 1 cut(s) 67
Bso31I GGTCTC 1 cut(s) 466
BsoBI CYCGRG 1 cut(s) 621
Bsp1286I GDGCHC 1 cut(s) 628
Bsp13I TCCGGA 1 cut(s) 860
Bsp143I GATC 2 cut(s) 930, 1026
Bsp19I CCATGG 2 cut(s) 636, 939
BspACI CCGC 8 cut(s) 17, 19, 90, 177, 315, 452, 455, 986
BspANI GGCC 1 cut(s) 67
BspEI TCCGGA 1 cut(s) 860
BspFNI CGCG 2 cut(s) 19, 315
BspLI GGNNCC 2 cut(s) 26, 438
BspMAI CTGCAG 1 cut(s) 312
BspTNI GGTCTC 1 cut(s) 466
BsrFI RCCGGY 2 cut(s) 191, 379
BssAI RCCGGY 2 cut(s) 191, 379
BssECI CCNNGG 6 cut(s) 17, 161, 296, 476, 636, 939
BssMI GATC 2 cut(s) 930, 1026
BssNI GRCGYC 1 cut(s) 911
BssT1I CCWWGG 3 cut(s) 296, 636, 939
Bst4CI ACNGT 4 cut(s) 217, 417, 433, 970
Bst6I CTCTTC 2 cut(s) 64, 201
BstACI GRCGYC 1 cut(s) 911
BstC8I GCNNGC 2 cut(s) 142, 230
BstDEI CTNAG 2 cut(s) 686, 897
BstDSI CCRYGG 3 cut(s) 17, 636, 939
BstF5I GGATG 5 cut(s) 549, 711, 849, 949, 1132
BstFNI CGCG 2 cut(s) 19, 315
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 2 cut(s) 140, 315
BstKTI GATC 2 cut(s) 933, 1029
BstMAI GTCTC 4 cut(s) 390, 411, 466, 1104
BstMBI GATC 2 cut(s) 930, 1026
BstMCI CGRYCG 2 cut(s) 167, 1074
BstMWI GCNNNNNNNGC 7 cut(s) 87, 287, 461, 632, 641, 988, 997
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 2 cut(s) 161, 409
BstSFI CTRYAG 2 cut(s) 308, 791
BstUI CGCG 2 cut(s) 19, 315
BstV1I GCAGC 6 cut(s) 93, 294, 322, 619, 987, 1000
BstV2I GAAGAC 1 cut(s) 65
BsuRI GGCC 1 cut(s) 67
BtgI CCRYGG 3 cut(s) 17, 636, 939
BtgZI GCGATG 1 cut(s) 941
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 5 cut(s) 549, 711, 849, 949, 1132
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 2 cut(s) 465, 682
Cac8I GCNNGC 2 cut(s) 142, 230
CfoI GCGC 2 cut(s) 140, 315
Cfr10I RCCGGY 2 cut(s) 191, 379
Cfr13I GGNCC 2 cut(s) 65, 437
Cfr42I CCGCGG 1 cut(s) 20
CseI GACGC 1 cut(s) 900
Csp6I GTAC 2 cut(s) 99, 775
CspAI ACCGGT 1 cut(s) 191
CviAII CATG 6 cut(s) 229, 426, 637, 940, 949, 1009
CviQI GTAC 2 cut(s) 99, 775
DdeI CTNAG 2 cut(s) 686, 897
DpnI GATC 2 cut(s) 932, 1028
DpnII GATC 2 cut(s) 930, 1026
Eam1104I CTCTTC 2 cut(s) 64, 201
EarI CTCTTC 2 cut(s) 64, 201
Ecl136II GAGCTC 1 cut(s) 626
Eco130I CCWWGG 3 cut(s) 296, 636, 939
Eco24I GRGCYC 1 cut(s) 628
Eco31I GGTCTC 1 cut(s) 466
Eco47I GGWCC 1 cut(s) 437
Eco53kI GAGCTC 1 cut(s) 626
Eco88I CYCGRG 1 cut(s) 621
EcoICRI GAGCTC 1 cut(s) 626
EcoT14I CCWWGG 3 cut(s) 296, 636, 939
EcoT38I GRGCYC 1 cut(s) 628
ErhI CCWWGG 3 cut(s) 296, 636, 939
FaeI CATG 6 cut(s) 232, 429, 640, 943, 952, 1012
FalI AAGNNNNNCTT 2 cut(s) 85, 117
FaqI GGGAC 2 cut(s) 426, 450
FatI CATG 6 cut(s) 228, 425, 636, 939, 948, 1008
FauI CCCGC 3 cut(s) 24, 308, 993
FbaI TGATCA 2 cut(s) 930, 1026
Fnu4HI GCNGC 8 cut(s) 82, 308, 311, 453, 456, 633, 989, 1001
FokI GGATG 5 cut(s) 556, 698, 856, 956, 1119
FriOI GRGCYC 1 cut(s) 628
Fsp4HI GCNGC 8 cut(s) 82, 308, 311, 453, 456, 633, 989, 1001
FspBI CTAG 7 cut(s) 225, 297, 324, 330, 348, 489, 716
GlaI GCGC 2 cut(s) 139, 314
GluI GCNGC 8 cut(s) 82, 308, 311, 453, 456, 633, 989, 1001
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 1 cut(s) 67
HapII CCGG 7 cut(s) 107, 163, 192, 380, 410, 440, 861
HgaI GACGC 1 cut(s) 900
HhaI GCGC 2 cut(s) 140, 315
Hin1I GRCGYC 1 cut(s) 911
Hin1II CATG 6 cut(s) 232, 429, 640, 943, 952, 1012
Hin6I GCGC 2 cut(s) 138, 313
HinP1I GCGC 2 cut(s) 138, 313
HinfI GANTC 3 cut(s) 150, 337, 830
HpaII CCGG 7 cut(s) 107, 163, 192, 380, 410, 440, 861
HphI GGTGA 3 cut(s) 145, 493, 859
Hpy166II GTNNAC 3 cut(s) 174, 341, 775
Hpy188I TCNGA 5 cut(s) 135, 276, 336, 534, 579
Hpy188III TCNNGA 3 cut(s) 186, 827, 861
Hpy8I GTNNAC 3 cut(s) 174, 341, 775
Hpy99I CGWCG 1 cut(s) 364
HpyAV CCTTC 5 cut(s) 232, 251, 358, 399, 751
HpyCH4III ACNGT 4 cut(s) 217, 417, 433, 970
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 7 cut(s) 232, 310, 429, 586, 659, 730, 736
HpyF10VI GCNNNNNNNGC 7 cut(s) 87, 287, 461, 632, 641, 988, 997
HpyF3I CTNAG 2 cut(s) 686, 897
HpySE526I ACGT 1 cut(s) 44
Hsp92I GRCGYC 1 cut(s) 911
Hsp92II CATG 6 cut(s) 232, 429, 640, 943, 952, 1012
HspAI GCGC 2 cut(s) 138, 313
Kpn2I TCCGGA 1 cut(s) 860
Ksp22I TGATCA 2 cut(s) 930, 1026
KspI CCGCGG 1 cut(s) 20
Kzo9I GATC 2 cut(s) 930, 1026
LmnI GCTCC 5 cut(s) 30, 442, 603, 987, 1018
Lsp1109I GCAGC 6 cut(s) 93, 294, 322, 619, 987, 1000
LweI GCATC 2 cut(s) 757, 883
MaeI CTAG 7 cut(s) 225, 297, 324, 330, 348, 489, 716
MaeII ACGT 1 cut(s) 44
MaeIII GTNAC 5 cut(s) 45, 194, 217, 970, 1012
MalI GATC 2 cut(s) 932, 1028
MboI GATC 2 cut(s) 930, 1026
MboII GAAGA 4 cut(s) 51, 65, 218, 547
MhlI GDGCHC 1 cut(s) 628
MluCI AATT 8 cut(s) 690, 768, 783, 809, 864, 876, 977, 1049
MlyI GAGTC 1 cut(s) 346
MroI TCCGGA 1 cut(s) 860
MslI CAYNNNNRTG 1 cut(s) 938
MspA1I CMGCKG 1 cut(s) 19
MspI CCGG 7 cut(s) 107, 163, 192, 380, 410, 440, 861
MspR9I CCNGG 2 cut(s) 163, 411
Mva1269I GAATGC 1 cut(s) 283
MvnI CGCG 2 cut(s) 19, 315
MwoI GCNNNNNNNGC 7 cut(s) 87, 287, 461, 632, 641, 988, 997
NciI CCSGG 2 cut(s) 163, 411
NcoI CCATGG 2 cut(s) 636, 939
NdeII GATC 2 cut(s) 930, 1026
NlaIII CATG 6 cut(s) 232, 429, 640, 943, 952, 1012
NlaIV GGNNCC 2 cut(s) 26, 438
NmeAIII GCCGAG 1 cut(s) 495
NmuCI GTSAC 2 cut(s) 45, 970
NspI RCATGY 1 cut(s) 232
PaeI GCATGC 1 cut(s) 232
PaeR7I CTCGAG 1 cut(s) 621
PcsI WCGNNNNNNNCGW 2 cut(s) 398, 473
PctI GAATGC 1 cut(s) 283
PfeI GAWTC 2 cut(s) 150, 830
PfoI TCCNGGA 1 cut(s) 409
PinAI ACCGGT 1 cut(s) 191
PkrI GCNGC 8 cut(s) 83, 309, 312, 454, 457, 634, 990, 1002
PleI GAGTC 1 cut(s) 345
PpsI GAGTC 1 cut(s) 345
Psp124BI GAGCTC 1 cut(s) 628
PspN4I GGNNCC 2 cut(s) 26, 438
PspPI GGNCC 2 cut(s) 65, 437
PspXI VCTCGAGB 1 cut(s) 621
PstI CTGCAG 1 cut(s) 312
RsaI GTAC 2 cut(s) 100, 776
RsaNI GTAC 2 cut(s) 99, 775
RseI CAYNNNNRTG 1 cut(s) 938
SacI GAGCTC 1 cut(s) 628
SacII CCGCGG 1 cut(s) 20
SatI GCNGC 8 cut(s) 82, 308, 311, 453, 456, 633, 989, 1001
Sau3AI GATC 2 cut(s) 930, 1026
Sau96I GGNCC 2 cut(s) 65, 437
ScaI AGTACT 1 cut(s) 100
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 2 cut(s) 163, 411
SduI GDGCHC 1 cut(s) 628
SfaNI GCATC 2 cut(s) 757, 883
SfcI CTRYAG 2 cut(s) 308, 791
Sfr274I CTCGAG 1 cut(s) 621
Sfr303I CCGCGG 1 cut(s) 20
SgrAI CRCCGGYG 1 cut(s) 379
SgrBI CCGCGG 1 cut(s) 20
SinI GGWCC 1 cut(s) 437
SlaI CTCGAG 1 cut(s) 621
SmiMI CAYNNNNRTG 1 cut(s) 938
SmlI CTYRAG 3 cut(s) 621, 627, 743
SmoI CTYRAG 3 cut(s) 621, 627, 743
SphI GCATGC 1 cut(s) 232
Sse9I AATT 8 cut(s) 690, 768, 783, 809, 864, 876, 977, 1049
SsiI CCGC 8 cut(s) 17, 19, 90, 177, 315, 452, 455, 986
SspMI CTAG 7 cut(s) 225, 297, 324, 330, 348, 489, 716
SstI GAGCTC 1 cut(s) 628
StyD4I CCNGG 2 cut(s) 161, 409
StyI CCWWGG 3 cut(s) 296, 636, 939
TaaI ACNGT 4 cut(s) 217, 417, 433, 970
TaiI ACGT 1 cut(s) 47
TaqI TCGA 4 cut(s) 167, 359, 622, 993
TaqII GACCGA 2 cut(s) 490, 1060
TasI AATT 8 cut(s) 690, 768, 783, 809, 864, 876, 977, 1049
TatI WGTACW 1 cut(s) 98
TauI GCSGC 2 cut(s) 455, 458
TfiI GAWTC 2 cut(s) 150, 830
TscAI CASTG 2 cut(s) 465, 682
TseFI GTSAC 2 cut(s) 45, 970
TseI GCWGC 6 cut(s) 81, 307, 310, 632, 988, 1000
Tsp45I GTSAC 2 cut(s) 45, 970
TspDTI ATGAA 2 cut(s) 219, 557
TspGWI ACGGA 1 cut(s) 1031
TspRI CASTG 2 cut(s) 465, 682
VpaK11BI GGWCC 1 cut(s) 437
XapI RAATTY 3 cut(s) 690, 783, 864
XceI RCATGY 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 1 cut(s) 946
XhoI CTCGAG 1 cut(s) 621
XmaJI CCTAGG 1 cut(s) 296
XspI CTAG 7 cut(s) 225, 297, 324, 330, 348, 489, 716
ZrmI AGTACT 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.