Rh1DG386300

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0010964
Physical Location & Seq
Reverse (-)
60602034 .. 60603615
1582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG386300.1

Sequence Viewer

Length: 1209 bp
ATGGAAAACACATACCCGCGGAGGCTCCAATCACATACAGCCACGTCACAACAGCACTCTTCTGGGCCTCGTCTTCTACGCAGCAGAAGCGGAAGTAGTACTTCTCCGGCGATAAACAACTCGTTATACTCTCCGAGCCCCAGCCACAGATTCACCACTCCCCGGTCGAAGTCCACCGCCAAATCCAGAACCGGTTACAGAGATGAAGAGAAAACGGTAACTTCAAGCATGCAAAAGAAGGGGCAAGAAAATAGAGAAGGTAAAAATAATAGTCAGAATGCTACTTTTGCTATCCCTAGGTTGTTGCTGCAGCGCGGGAATACTAGCCCTAGTCCGAGTCCACGCCCTAGCTTTGGCTCGACGAAGGGGAGGAAGTCGCCGGTGTCTCCTTCGGCTTGGGCGTTGTCTCCGGGACGGTCTTTGCCATGCACGGTAGGACCGGAGCTGACTGCGGCGGCAGTGGCTTCGCCGAGACCGAGGGTGAAGTCTAGTGGGGGTGGAGTTGGTGGGGTTTTGAAGTATTTTAGGCTTCCGAAGAAAGGGATGACTATACAAGAGGAGGAGTTTCATCAGTTTCGGATTTTGCATAATAGGTTGCTCCAATGGAGGTTTGCCAATGCTCGAGCTCAAGCTGCCATGGTTGCTTCACACAAAGTTGCACAGGACAGAATGTTCAGTGTGTGGCTTAGAATTTTCAAAATGAGGAACTACATCCTAGATAAGCAACTACAACTGCAAAAGCTCAAGCATCAAGTGAAGGTGTATCAAATTGTGTACCCTCAAATTTCTCTACTGAACGAATGGGGGAAATTGGAGAGAAAAAATCAAGAATCTGTGAGCAGGATGGTGAGGAAGTTATCCGGAATTTCAAATACAATTCCTTTGGTAAATGATGCTAAGGGAGATGTGGCGTCTATATACGAAGCGATGATCACGGCCATGGATGTCATGGCTGGTATTCAAGCAACGGTCACTAAATTGCTCCCGCAGCTGGAAAAGCTGCTCTACATGGTTACGGAGCTTTTGATCACGCAGAAGCAACAAAGAGAATTAGAGAAATATTTAACATCGGTCGTTGGTCTTATGAATCTGTGCCTAAAGCTTTTTCACCAGGGAGTAACTATTGTTCAAGTAAAACTAAAAATGGAGGAGCTTCGTCATAGACATAAGATATTAGGTGCAACCAACCCAAACGGAGCTTGTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

402

Amino Acids

45.07

Weight (kDa)

10.49

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 32 - 351 3.6e-69 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 19, 315
AccIII TCCGGA 1 cut(s) 860
AciI CCGC 8 cut(s) 17, 19, 90, 177, 315, 452, 455, 986
AcoI YGGCCR 1 cut(s) 936
AcsI RAATTY 3 cut(s) 690, 783, 864
AcyI GRCGYC 1 cut(s) 911
AfaI GTAC 2 cut(s) 100, 776
AfiI CCNNNNNNNGG 4 cut(s) 162, 353, 539, 991
AgeI ACCGGT 1 cut(s) 191
AgsI TTSAA 6 cut(s) 225, 517, 697, 870, 962, 1130
AjiI CACGTC 1 cut(s) 45
AjnI CCWGG 1 cut(s) 1110
AloI GAACNNNNNNTCC 2 cut(s) 656, 688
Alw21I GWGCWC 1 cut(s) 628
Alw26I GTCTC 3 cut(s) 390, 411, 466
Ama87I CYCGRG 1 cut(s) 621
Aor13HI TCCGGA 1 cut(s) 860
AoxI GGCC 2 cut(s) 65, 936
ApeKI GCWGC 6 cut(s) 81, 307, 310, 632, 988, 1000
ApoI RAATTY 3 cut(s) 690, 783, 864
AsiGI ACCGGT 1 cut(s) 191
AspA2I CCTAGG 1 cut(s) 296
AspLEI GCGC 1 cut(s) 315
AspS9I GGNCC 2 cut(s) 65, 437
AsuC2I CCSGG 2 cut(s) 163, 411
AsuHPI GGTGA 4 cut(s) 145, 493, 859, 1100
AvaI CYCGRG 1 cut(s) 621
AvaII GGWCC 1 cut(s) 437
AvrII CCTAGG 1 cut(s) 296
BanII GRGCYC 2 cut(s) 140, 628
BbsI GAAGAC 1 cut(s) 65
Bbv12I GWGCWC 1 cut(s) 628
BbvI GCAGC 6 cut(s) 93, 294, 322, 619, 987, 1000
BccI CCATC 1 cut(s) 838
BceAI ACGGC 1 cut(s) 951
BcgI CGANNNNNNTGC 2 cut(s) 447, 481
BciT130I CCWGG 1 cut(s) 1112
BclI TGATCA 2 cut(s) 930, 1026
BcnI CCSGG 2 cut(s) 163, 411
BcoDI GTCTC 3 cut(s) 390, 411, 466
BfaI CTAG 6 cut(s) 297, 324, 330, 348, 489, 716
BfmI CTRYAG 1 cut(s) 308
BisI GCNGC 8 cut(s) 82, 308, 311, 453, 456, 633, 989, 1001
BlnI CCTAGG 1 cut(s) 296
BlsI GCNGC 8 cut(s) 83, 309, 312, 454, 457, 634, 990, 1002
BmcAI AGTACT 1 cut(s) 100
Bme1390I CCNGG 3 cut(s) 163, 411, 1112
Bme18I GGWCC 1 cut(s) 437
BmeT110I CYCGRG 1 cut(s) 621
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 2 cut(s) 65, 437
BmiI GGNNCC 1 cut(s) 26
BmrFI CCNGG 3 cut(s) 163, 411, 1112
BmsI GCATC 2 cut(s) 757, 883
BpiI GAAGAC 1 cut(s) 65
Bpu10I CCTNAGC 1 cut(s) 897
BpuEI CTTGAG 2 cut(s) 612, 728
BpuMI CCSGG 2 cut(s) 163, 411
BsaHI GRCGYC 1 cut(s) 911
BsaI GGTCTC 1 cut(s) 466
BsaJI CCNNGG 7 cut(s) 17, 161, 296, 476, 636, 939, 1111
BsaWI WCCGGW 3 cut(s) 191, 439, 860
Bsc4I CCNNNNNNNGG 4 cut(s) 162, 353, 539, 991
Bse118I RCCGGY 2 cut(s) 191, 379
BseAI TCCGGA 1 cut(s) 860
BseBI CCWGG 1 cut(s) 1112
BseDI CCNNGG 7 cut(s) 17, 161, 296, 476, 636, 939, 1111
BseGI GGATG 4 cut(s) 549, 711, 849, 949
BseLI CCNNNNNNNGG 4 cut(s) 162, 353, 539, 991
BseRI GAGGAG 3 cut(s) 572, 575, 1163
BseXI GCAGC 6 cut(s) 93, 294, 322, 619, 987, 1000
BseYI CCCAGC 1 cut(s) 140
Bsh1236I CGCG 2 cut(s) 19, 315
Bsh1285I CGRYCG 2 cut(s) 167, 1074
BshFI GGCC 2 cut(s) 67, 938
BshTI ACCGGT 1 cut(s) 191
BsiEI CGRYCG 2 cut(s) 167, 1074
BsiHKAI GWGCWC 1 cut(s) 628
BsiHKCI CYCGRG 1 cut(s) 621
BsiSI CCGG 7 cut(s) 107, 163, 192, 380, 410, 440, 861
BslFI GGGAC 1 cut(s) 426
BslI CCNNNNNNNGG 4 cut(s) 162, 353, 539, 991
BsmAI GTCTC 3 cut(s) 390, 411, 466
BsmFI GGGAC 1 cut(s) 426
BsmI GAATGC 1 cut(s) 283
BsnI GGCC 2 cut(s) 67, 938
Bso31I GGTCTC 1 cut(s) 466
BsoBI CYCGRG 1 cut(s) 621
Bsp1286I GDGCHC 2 cut(s) 140, 628
Bsp13I TCCGGA 1 cut(s) 860
Bsp143I GATC 2 cut(s) 930, 1026
Bsp19I CCATGG 2 cut(s) 636, 939
BspACI CCGC 8 cut(s) 17, 19, 90, 177, 315, 452, 455, 986
BspANI GGCC 2 cut(s) 67, 938
BspEI TCCGGA 1 cut(s) 860
BspFNI CGCG 2 cut(s) 19, 315
BspLI GGNNCC 1 cut(s) 26
BspMAI CTGCAG 1 cut(s) 312
BspTNI GGTCTC 1 cut(s) 466
BsrFI RCCGGY 2 cut(s) 191, 379
BssAI RCCGGY 2 cut(s) 191, 379
BssECI CCNNGG 7 cut(s) 17, 161, 296, 476, 636, 939, 1111
BssMI GATC 2 cut(s) 930, 1026
BssNI GRCGYC 1 cut(s) 911
BssT1I CCWWGG 3 cut(s) 296, 636, 939
Bst2UI CCWGG 1 cut(s) 1112
Bst4CI ACNGT 4 cut(s) 217, 417, 433, 970
Bst6I CTCTTC 2 cut(s) 64, 201
BstACI GRCGYC 1 cut(s) 911
BstC8I GCNNGC 1 cut(s) 230
BstDEI CTNAG 2 cut(s) 686, 897
BstDSI CCRYGG 3 cut(s) 17, 636, 939
BstF5I GGATG 4 cut(s) 549, 711, 849, 949
BstFNI CGCG 2 cut(s) 19, 315
BstHHI GCGC 1 cut(s) 315
BstKTI GATC 2 cut(s) 933, 1029
BstMAI GTCTC 3 cut(s) 390, 411, 466
BstMBI GATC 2 cut(s) 930, 1026
BstMCI CGRYCG 2 cut(s) 167, 1074
BstMWI GCNNNNNNNGC 7 cut(s) 87, 287, 461, 632, 641, 988, 997
BstNI CCWGG 1 cut(s) 1112
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 3 cut(s) 161, 409, 1110
BstSFI CTRYAG 1 cut(s) 308
BstUI CGCG 2 cut(s) 19, 315
BstV1I GCAGC 6 cut(s) 93, 294, 322, 619, 987, 1000
BstV2I GAAGAC 1 cut(s) 65
BsuRI GGCC 2 cut(s) 67, 938
BtgI CCRYGG 3 cut(s) 17, 636, 939
BtgZI GCGATG 1 cut(s) 941
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 4 cut(s) 549, 711, 849, 949
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 2 cut(s) 465, 682
Cac8I GCNNGC 1 cut(s) 230
CfoI GCGC 1 cut(s) 315
Cfr10I RCCGGY 2 cut(s) 191, 379
Cfr13I GGNCC 2 cut(s) 65, 437
Cfr42I CCGCGG 1 cut(s) 20
CseI GACGC 1 cut(s) 900
Csp6I GTAC 2 cut(s) 99, 775
CspAI ACCGGT 1 cut(s) 191
CviAII CATG 6 cut(s) 229, 426, 637, 940, 949, 1009
CviQI GTAC 2 cut(s) 99, 775
DdeI CTNAG 2 cut(s) 686, 897
DpnI GATC 2 cut(s) 932, 1028
DpnII GATC 2 cut(s) 930, 1026
EaeI YGGCCR 1 cut(s) 936
Eam1104I CTCTTC 2 cut(s) 64, 201
EarI CTCTTC 2 cut(s) 64, 201
Ecl136II GAGCTC 1 cut(s) 626
Eco130I CCWWGG 3 cut(s) 296, 636, 939
Eco24I GRGCYC 2 cut(s) 140, 628
Eco31I GGTCTC 1 cut(s) 466
Eco47I GGWCC 1 cut(s) 437
Eco53kI GAGCTC 1 cut(s) 626
Eco88I CYCGRG 1 cut(s) 621
EcoICRI GAGCTC 1 cut(s) 626
EcoRII CCWGG 1 cut(s) 1110
EcoT14I CCWWGG 3 cut(s) 296, 636, 939
EcoT38I GRGCYC 2 cut(s) 140, 628
ErhI CCWWGG 3 cut(s) 296, 636, 939
FaeI CATG 6 cut(s) 232, 429, 640, 943, 952, 1012
FalI AAGNNNNNCTT 2 cut(s) 85, 117
FaqI GGGAC 1 cut(s) 426
FatI CATG 6 cut(s) 228, 425, 636, 939, 948, 1008
FauI CCCGC 3 cut(s) 24, 308, 993
FbaI TGATCA 2 cut(s) 930, 1026
Fnu4HI GCNGC 8 cut(s) 82, 308, 311, 453, 456, 633, 989, 1001
FokI GGATG 4 cut(s) 556, 698, 856, 956
FriOI GRGCYC 2 cut(s) 140, 628
Fsp4HI GCNGC 8 cut(s) 82, 308, 311, 453, 456, 633, 989, 1001
FspBI CTAG 6 cut(s) 297, 324, 330, 348, 489, 716
GlaI GCGC 1 cut(s) 314
GluI GCNGC 8 cut(s) 82, 308, 311, 453, 456, 633, 989, 1001
GsaI CCCAGC 1 cut(s) 144
HaeIII GGCC 2 cut(s) 67, 938
HapII CCGG 7 cut(s) 107, 163, 192, 380, 410, 440, 861
HgaI GACGC 1 cut(s) 900
HhaI GCGC 1 cut(s) 315
Hin1I GRCGYC 1 cut(s) 911
Hin1II CATG 6 cut(s) 232, 429, 640, 943, 952, 1012
Hin6I GCGC 1 cut(s) 313
HinP1I GCGC 1 cut(s) 313
HindIII AAGCTT 1 cut(s) 1100
HinfI GANTC 4 cut(s) 150, 337, 830, 1087
HpaII CCGG 7 cut(s) 107, 163, 192, 380, 410, 440, 861
HphI GGTGA 4 cut(s) 145, 493, 859, 1100
Hpy166II GTNNAC 3 cut(s) 174, 341, 775
Hpy188I TCNGA 5 cut(s) 135, 276, 336, 534, 579
Hpy188III TCNNGA 3 cut(s) 186, 827, 861
Hpy8I GTNNAC 3 cut(s) 174, 341, 775
Hpy99I CGWCG 1 cut(s) 364
HpyAV CCTTC 5 cut(s) 232, 251, 358, 399, 751
HpyCH4III ACNGT 4 cut(s) 217, 417, 433, 970
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 7 cut(s) 232, 310, 429, 586, 659, 736, 1181
HpyF10VI GCNNNNNNNGC 7 cut(s) 87, 287, 461, 632, 641, 988, 997
HpyF3I CTNAG 2 cut(s) 686, 897
HpySE526I ACGT 1 cut(s) 44
Hsp92I GRCGYC 1 cut(s) 911
Hsp92II CATG 6 cut(s) 232, 429, 640, 943, 952, 1012
HspAI GCGC 1 cut(s) 313
Kpn2I TCCGGA 1 cut(s) 860
Ksp22I TGATCA 2 cut(s) 930, 1026
KspI CCGCGG 1 cut(s) 20
Kzo9I GATC 2 cut(s) 930, 1026
LmnI GCTCC 7 cut(s) 30, 442, 603, 987, 1018, 1150, 1196
Lsp1109I GCAGC 6 cut(s) 93, 294, 322, 619, 987, 1000
LweI GCATC 2 cut(s) 757, 883
MaeI CTAG 6 cut(s) 297, 324, 330, 348, 489, 716
MaeII ACGT 1 cut(s) 44
MaeIII GTNAC 6 cut(s) 45, 194, 217, 970, 1012, 1117
MalI GATC 2 cut(s) 932, 1028
MboI GATC 2 cut(s) 930, 1026
MboII GAAGA 4 cut(s) 51, 65, 218, 547
MhlI GDGCHC 2 cut(s) 140, 628
MluCI AATT 8 cut(s) 690, 768, 783, 809, 864, 876, 977, 1049
MlyI GAGTC 1 cut(s) 346
MroI TCCGGA 1 cut(s) 860
MseI TTAA 1 cut(s) 1064
MslI CAYNNNNRTG 1 cut(s) 938
MspA1I CMGCKG 2 cut(s) 19, 991
MspI CCGG 7 cut(s) 107, 163, 192, 380, 410, 440, 861
MspR9I CCNGG 3 cut(s) 163, 411, 1112
Mva1269I GAATGC 1 cut(s) 283
MvaI CCWGG 1 cut(s) 1112
MvnI CGCG 2 cut(s) 19, 315
MwoI GCNNNNNNNGC 7 cut(s) 87, 287, 461, 632, 641, 988, 997
NciI CCSGG 2 cut(s) 163, 411
NcoI CCATGG 2 cut(s) 636, 939
NdeII GATC 2 cut(s) 930, 1026
NlaIII CATG 6 cut(s) 232, 429, 640, 943, 952, 1012
NlaIV GGNNCC 1 cut(s) 26
NmeAIII GCCGAG 1 cut(s) 495
NmuCI GTSAC 2 cut(s) 45, 970
NspI RCATGY 1 cut(s) 232
PaeI GCATGC 1 cut(s) 232
PaeR7I CTCGAG 1 cut(s) 621
PcsI WCGNNNNNNNCGW 2 cut(s) 398, 473
PctI GAATGC 1 cut(s) 283
PfeI GAWTC 3 cut(s) 150, 830, 1087
PfoI TCCNGGA 1 cut(s) 409
PinAI ACCGGT 1 cut(s) 191
PkrI GCNGC 8 cut(s) 83, 309, 312, 454, 457, 634, 990, 1002
PleI GAGTC 1 cut(s) 345
PpsI GAGTC 1 cut(s) 345
Psp124BI GAGCTC 1 cut(s) 628
Psp6I CCWGG 1 cut(s) 1110
PspFI CCCAGC 1 cut(s) 140
PspGI CCWGG 1 cut(s) 1110
PspN4I GGNNCC 1 cut(s) 26
PspPI GGNCC 2 cut(s) 65, 437
PspXI VCTCGAGB 1 cut(s) 621
PsrI GAACNNNNNNTAC 2 cut(s) 1110, 1142
PstI CTGCAG 1 cut(s) 312
PvuII CAGCTG 1 cut(s) 991
RsaI GTAC 2 cut(s) 100, 776
RsaNI GTAC 2 cut(s) 99, 775
RseI CAYNNNNRTG 1 cut(s) 938
SacI GAGCTC 1 cut(s) 628
SacII CCGCGG 1 cut(s) 20
SaqAI TTAA 1 cut(s) 1064
SatI GCNGC 8 cut(s) 82, 308, 311, 453, 456, 633, 989, 1001
Sau3AI GATC 2 cut(s) 930, 1026
Sau96I GGNCC 2 cut(s) 65, 437
ScaI AGTACT 1 cut(s) 100
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 3 cut(s) 163, 411, 1112
SduI GDGCHC 2 cut(s) 140, 628
SfaNI GCATC 2 cut(s) 757, 883
SfcI CTRYAG 1 cut(s) 308
Sfr274I CTCGAG 1 cut(s) 621
Sfr303I CCGCGG 1 cut(s) 20
SgrAI CRCCGGYG 1 cut(s) 379
SgrBI CCGCGG 1 cut(s) 20
SinI GGWCC 1 cut(s) 437
SlaI CTCGAG 1 cut(s) 621
SmiMI CAYNNNNRTG 1 cut(s) 938
SmlI CTYRAG 3 cut(s) 621, 627, 743
SmoI CTYRAG 3 cut(s) 621, 627, 743
SphI GCATGC 1 cut(s) 232
Sse9I AATT 8 cut(s) 690, 768, 783, 809, 864, 876, 977, 1049
SsiI CCGC 8 cut(s) 17, 19, 90, 177, 315, 452, 455, 986
SspI AATATT 1 cut(s) 1061
SspMI CTAG 6 cut(s) 297, 324, 330, 348, 489, 716
SstI GAGCTC 1 cut(s) 628
StyD4I CCNGG 3 cut(s) 161, 409, 1110
StyI CCWWGG 3 cut(s) 296, 636, 939
TaaI ACNGT 4 cut(s) 217, 417, 433, 970
TaiI ACGT 1 cut(s) 47
TaqI TCGA 3 cut(s) 167, 359, 622
TaqII GACCGA 2 cut(s) 490, 1060
TasI AATT 8 cut(s) 690, 768, 783, 809, 864, 876, 977, 1049
TatI WGTACW 1 cut(s) 98
TauI GCSGC 2 cut(s) 455, 458
TfiI GAWTC 3 cut(s) 150, 830, 1087
Tru1I TTAA 1 cut(s) 1064
Tru9I TTAA 1 cut(s) 1064
TscAI CASTG 2 cut(s) 465, 682
TseFI GTSAC 2 cut(s) 45, 970
TseI GCWGC 6 cut(s) 81, 307, 310, 632, 988, 1000
Tsp45I GTSAC 2 cut(s) 45, 970
TspDTI ATGAA 3 cut(s) 219, 557, 1100
TspGWI ACGGA 2 cut(s) 1031, 1209
TspRI CASTG 2 cut(s) 465, 682
VpaK11BI GGWCC 1 cut(s) 437
XapI RAATTY 3 cut(s) 690, 783, 864
XceI RCATGY 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 1 cut(s) 946
XhoI CTCGAG 1 cut(s) 621
XmaJI CCTAGG 1 cut(s) 296
XspI CTAG 6 cut(s) 297, 324, 330, 348, 489, 716
ZrmI AGTACT 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.