AT4G28420

S-alkyl-thiohydroximate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004272
Physical Location & Seq
Forward (+)
14055661 .. 14057592
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G28420.2

Sequence Viewer

Length: 1170 bp
ATGAACCACAACAGCAACCTCGTTCTTCCTTCCCATCAAACCGAGACGCAGACGCAAGATGAAACCGATATCAGCGTTTGGCGTTTCAGGGGAAGCGACAACGCAGCCAAAGCCTCCAGCGTCACGATGAGAGTCATCGTCTACAAGCTCTTCGATGAATGCAGCCTGGATGTGAAAAAGCCTCTTTTACCCCTTGCTCACGGTGACCCTTCTGTCTACCCTTGTTACCGCACCTCCATCCTCGTCGAGAACGCCGTGGTCGACGTCCTTCGCTCCGGCAAGGGTAACTCTTACGGCCCCGCCGCCGGAATTCTCCCAGCCAGACAGGCGGTCGCAGATTACGTGAACCGAGACTTGACGAACAAGGTAAAGCCTAATGATGTATTCATAACTGTCGGATGCAACCAAGGGATAGAAGTAGTTCTTCAGTCACTGGCTCGACCAAACGCTAACATCCTTCTCCCAAGGCCTAGTTACCCTCACTACGAGGCTCGTGCCGTCTACAGTGGACTCGAGGTCCGCAAGTTCGATCTCCTTCCAGAGAAAGAATGGGAGATTGATCTCCCAGGCATCGAAGCCATGGCAGATGAGAACACAGTTGCAATGGTTATCATAAACCCTAACAACCCCTGTGGAAATGTTTACTCTTACGATCATCTTAAGAAGGTGGCGGAGACGGCTAAGAAGCTGGGAATAATGGTAATCACAGACGAAGTTTATTGCCAAACAATCTTCGGAGACAAACCTTTTGTCCCAATGGGGGAGTTTTCTTCGATAACTCCGGTTATCACTTTGGGCGGTATATCGAAAGGATGGATTGTTCCTGGTTGGAGAATTGGCTGGATCGCTTTGAATGACCCCAGAGGAATTCTCAAGTCCACAGGGATGGTACAGTCCATTCAACAGAACCTTGACATAACTCCAGATGCTACAACTATTGTTCAAGCTGCACTTCCTGAGATTTTAGGGAAAGCGAATAAAGAGTTGTTTGCCAAGAAGAATTCGATGCTGAAACAAAACGTGGAATTGGTCTGTGATAGGCTCAAGGAGATTCCTTGCTTGGTCTGCAACAAGAAACCTGAGTCATGCACTTACTTATTGGTACCACCACCAACGTCTCTTATACTCTTTCATGATTCTAGATCAATTTGGATGATTCTTATTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001558 GO:0001560 GO:0003674 GO:0003824 GO:0004121 GO:0004838 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006082 GO:0006520 GO:0006570 GO:0006572 GO:0006725 GO:0006766 GO:0006775 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0007154 GO:0007275 GO:0008150 GO:0008152 GO:0008483 GO:0009056 GO:0009058 GO:0009063 GO:0009072 GO:0009074 GO:0009110 GO:0009605 GO:0009611 GO:0009628 GO:0009636 GO:0009651 GO:0009683 GO:0009684 GO:0009719 GO:0009725 GO:0009737 GO:0009753 GO:0009850 GO:0009851 GO:0009987 GO:0009991 GO:0010033 GO:0010188 GO:0010189 GO:0010817 GO:0016020 GO:0016053 GO:0016054 GO:0016143 GO:0016144 GO:0016740 GO:0016769 GO:0016829 GO:0016846 GO:0018130 GO:0019438 GO:0019439 GO:0019748 GO:0019752 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022622 GO:0031668 GO:0032501 GO:0032502 GO:0032787 GO:0033993 GO:0034641 GO:0034754 GO:0040008 GO:0042221 GO:0042360 GO:0042362 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042538 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046394 GO:0046395 GO:0046483 GO:0048046 GO:0048364 GO:0048731 GO:0048830 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051716 GO:0065007 GO:0065008 GO:0070547 GO:0071496 GO:0071704 GO:0072330 GO:0080108 GO:0097305 GO:0099402 GO:1901135 GO:1901137 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901605 GO:1901606 GO:1901615 GO:1901617 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00130 ko00270 ko00350 ko00360 ko00380 ko00400 ko00401 ko00950 ko00960 ko00966 ko01100 ko01110 ko01130 ko01210 ko01230 map00130 map00270 map00350 map00360 map00380 map00400 map00401 map00950 map00960 map00966 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

389

Amino Acids

43.02

Weight (kDa)

6.34

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 82 - 426 2.5e-53 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 212
AatII GACGTC 1 cut(s) 267
Acc65I GGTACC 1 cut(s) 1102
AccB1I GGYRCC 1 cut(s) 1102
AccI GTMKAC 4 cut(s) 141, 216, 261, 501
AciI CCGC 7 cut(s) 229, 300, 303, 329, 520, 671, 798
AclWI GGATC 1 cut(s) 851
AcsI RAATTY 3 cut(s) 309, 867, 1000
AcuI CTGAAG 1 cut(s) 410
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 2 cut(s) 891, 1104
AfiI CCNNNNNNNGG 2 cut(s) 305, 760
AflII CTTAAG 1 cut(s) 659
AgsI TTSAA 3 cut(s) 853, 902, 944
AhdI GACNNNNNGTC 2 cut(s) 329, 515
AjnI CCWGG 3 cut(s) 165, 565, 823
AloI GAACNNNNNNTCC 2 cut(s) 804, 836
AluBI AGCT 3 cut(s) 148, 688, 947
AluI AGCT 3 cut(s) 148, 688, 947
Alw26I GTCTC 5 cut(s) 38, 345, 668, 732, 1122
AlwI GGATC 1 cut(s) 851
AlwNI CAGNNNCTG 1 cut(s) 433
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 2 cut(s) 295, 467
ApeKI GCWGC 3 cut(s) 104, 162, 947
ApoI RAATTY 3 cut(s) 309, 867, 1000
ArsI GACNNNNNNTTYG 4 cut(s) 731, 735, 763, 767
Asp700I GAANNNNTTC 1 cut(s) 420
Asp718I GGTACC 1 cut(s) 1102
AspS9I GGNCC 2 cut(s) 296, 517
AsuHPI GGTGA 1 cut(s) 215
AvaI CYCGRG 1 cut(s) 512
AvaII GGWCC 1 cut(s) 517
BanI GGYRCC 1 cut(s) 1102
BauI CACGAG 1 cut(s) 492
BbvI GCAGC 3 cut(s) 116, 174, 934
BccI CCATC 4 cut(s) 42, 245, 807, 880
BceAI ACGGC 4 cut(s) 239, 310, 482, 693
BcgI CGANNNNNNTGC 2 cut(s) 476, 510
BciT130I CCWGG 3 cut(s) 167, 567, 825
BcoDI GTCTC 5 cut(s) 38, 345, 668, 732, 1122
BfaI CTAG 2 cut(s) 471, 1140
BfmI CTRYAG 1 cut(s) 502
BfrI CTTAAG 1 cut(s) 659
BglI GCCNNNNNGGC 1 cut(s) 326
BisI GCNGC 4 cut(s) 105, 163, 303, 948
BlsI GCNGC 4 cut(s) 106, 164, 304, 949
Bme1390I CCNGG 3 cut(s) 167, 567, 825
Bme18I GGWCC 1 cut(s) 517
BmeRI GACNNNNNGTC 2 cut(s) 329, 515
BmeT110I CYCGRG 1 cut(s) 512
BmgT120I GGNCC 2 cut(s) 296, 517
BmiI GGNNCC 2 cut(s) 298, 1104
BmrFI CCNGG 3 cut(s) 167, 567, 825
BmsI GCATC 4 cut(s) 389, 579, 916, 996
BplI GAGNNNNNCTC 2 cut(s) 855, 887
BpmI CTGGAG 2 cut(s) 100, 906
BpuEI CTTGAG 2 cut(s) 857, 1028
BsaAI YACGTR 1 cut(s) 343
BsaHI GRCGYC 1 cut(s) 264
BsaJI CCNNGG 5 cut(s) 255, 406, 464, 565, 579
BsaWI WCCGGW 1 cut(s) 781
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 2 cut(s) 305, 760
Bse1I ACTGG 1 cut(s) 438
Bse3DI GCAATG 1 cut(s) 609
BseBI CCWGG 3 cut(s) 167, 567, 825
BseDI CCNNGG 5 cut(s) 255, 406, 464, 565, 579
BseGI GGATG 7 cut(s) 175, 237, 404, 453, 818, 891, 1158
BseLI CCNNNNNNNGG 2 cut(s) 305, 760
BseMI GCAATG 1 cut(s) 609
BseMII CTCAG 2 cut(s) 948, 1071
BseNI ACTGG 1 cut(s) 438
BseXI GCAGC 3 cut(s) 116, 174, 934
BseYI CCCAGC 2 cut(s) 316, 688
BsgI GTGCAG 1 cut(s) 933
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 2 cut(s) 297, 469
BshNI GGYRCC 1 cut(s) 1102
BsiEI CGRYCG 1 cut(s) 333
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 3 cut(s) 276, 306, 782
BslFI GGGAC 1 cut(s) 737
BslI CCNNNNNNNGG 2 cut(s) 305, 760
BsmAI GTCTC 5 cut(s) 38, 345, 668, 732, 1122
BsmBI CGTCTC 3 cut(s) 38, 668, 1122
BsmFI GGGAC 1 cut(s) 737
BsmI GAATGC 1 cut(s) 164
BsnI GGCC 2 cut(s) 297, 469
BsoBI CYCGRG 1 cut(s) 512
Bsp143I GATC 5 cut(s) 529, 559, 652, 843, 1142
Bsp19I CCATGG 1 cut(s) 579
BspACI CCGC 7 cut(s) 229, 300, 303, 329, 520, 671, 798
BspANI GGCC 2 cut(s) 297, 469
BspCNI CTCAG 2 cut(s) 949, 1072
BspHI TCATGA 1 cut(s) 1132
BspLI GGNNCC 2 cut(s) 298, 1104
BspPI GGATC 1 cut(s) 851
BspQI GCTCTTC 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 1102
BspTI CTTAAG 1 cut(s) 659
BsrDI GCAATG 1 cut(s) 609
BsrI ACTGG 1 cut(s) 438
BssECI CCNNGG 5 cut(s) 255, 406, 464, 565, 579
BssMI GATC 5 cut(s) 529, 559, 652, 843, 1142
BssNI GRCGYC 1 cut(s) 264
BssSI CACGAG 1 cut(s) 492
BssT1I CCWWGG 3 cut(s) 406, 464, 579
Bst2BI CACGAG 1 cut(s) 492
Bst2UI CCWGG 3 cut(s) 167, 567, 825
Bst4CI ACNGT 5 cut(s) 203, 394, 506, 598, 894
Bst6I CTCTTC 1 cut(s) 155
BstACI GRCGYC 1 cut(s) 264
BstAFI CTTAAG 1 cut(s) 659
BstBAI YACGTR 1 cut(s) 343
BstDEI CTNAG 3 cut(s) 681, 957, 1080
BstDSI CCRYGG 2 cut(s) 255, 579
BstEII GGTNACC 1 cut(s) 203
BstF5I GGATG 7 cut(s) 175, 237, 404, 453, 818, 891, 1158
BstKTI GATC 5 cut(s) 532, 562, 655, 846, 1145
BstMAI GTCTC 5 cut(s) 38, 345, 668, 732, 1122
BstMBI GATC 5 cut(s) 529, 559, 652, 843, 1142
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 4 cut(s) 110, 326, 677, 1065
BstNI CCWGG 3 cut(s) 167, 567, 825
BstPI GGTNACC 1 cut(s) 203
BstSCI CCNGG 3 cut(s) 165, 565, 823
BstSFI CTRYAG 1 cut(s) 502
BstV1I GCAGC 3 cut(s) 116, 174, 934
BstXI CCANNNNNNTGG 1 cut(s) 886
BsuRI GGCC 2 cut(s) 297, 469
BtgI CCRYGG 2 cut(s) 255, 579
BtsCI GGATG 7 cut(s) 175, 237, 404, 453, 818, 891, 1158
BtsIMutI CAGTG 2 cut(s) 431, 511
CaiI CAGNNNCTG 1 cut(s) 433
CciI TCATGA 1 cut(s) 1132
Cfr13I GGNCC 2 cut(s) 296, 517
CseI GACGC 3 cut(s) 55, 61, 109
Csp6I GTAC 2 cut(s) 890, 1103
CspCI CAANNNNNGTGG 2 cut(s) 613, 648
CviAII CATG 3 cut(s) 580, 1086, 1133
CviQI GTAC 2 cut(s) 890, 1103
DdeI CTNAG 3 cut(s) 681, 957, 1080
DpnI GATC 5 cut(s) 531, 561, 654, 845, 1144
DpnII GATC 5 cut(s) 529, 559, 652, 843, 1142
DrdI GACNNNNNNGTC 1 cut(s) 212
DriI GACNNNNNGTC 2 cut(s) 329, 515
DseDI GACNNNNNNGTC 1 cut(s) 212
Eam1104I CTCTTC 1 cut(s) 155
Eam1105I GACNNNNNGTC 2 cut(s) 329, 515
EarI CTCTTC 1 cut(s) 155
EciI GGCGGA 1 cut(s) 686
Eco130I CCWWGG 3 cut(s) 406, 464, 579
Eco147I AGGCCT 1 cut(s) 469
Eco32I GATATC 1 cut(s) 70
Eco47I GGWCC 1 cut(s) 517
Eco57I CTGAAG 1 cut(s) 410
Eco88I CYCGRG 1 cut(s) 512
Eco91I GGTNACC 1 cut(s) 203
EcoO65I GGTNACC 1 cut(s) 203
EcoRI GAATTC 3 cut(s) 309, 867, 1000
EcoRII CCWGG 3 cut(s) 165, 565, 823
EcoRV GATATC 1 cut(s) 70
EcoT14I CCWWGG 3 cut(s) 406, 464, 579
ErhI CCWWGG 3 cut(s) 406, 464, 579
Esp3I CGTCTC 3 cut(s) 38, 668, 1122
FaeI CATG 3 cut(s) 583, 1089, 1136
FaiI YATR 8 cut(s) 389, 581, 614, 803, 917, 1087, 1124, 1134
FalI AAGNNNNNCTT 4 cut(s) 408, 440, 936, 968
FaqI GGGAC 1 cut(s) 737
FatI CATG 3 cut(s) 579, 1085, 1132
FauI CCCGC 1 cut(s) 307
FblI GTMKAC 4 cut(s) 141, 216, 261, 501
Fnu4HI GCNGC 4 cut(s) 105, 163, 303, 948
FokI GGATG 7 cut(s) 182, 224, 411, 440, 825, 898, 1165
Fsp4HI GCNGC 4 cut(s) 105, 163, 303, 948
FspBI CTAG 2 cut(s) 471, 1140
GluI GCNGC 4 cut(s) 105, 163, 303, 948
GsaI CCCAGC 2 cut(s) 320, 692
GsuI CTGGAG 2 cut(s) 100, 906
HaeIII GGCC 2 cut(s) 297, 469
HapII CCGG 3 cut(s) 276, 306, 782
HgaI GACGC 3 cut(s) 55, 61, 109
Hin1I GRCGYC 1 cut(s) 264
Hin1II CATG 3 cut(s) 583, 1089, 1136
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 6 cut(s) 132, 510, 1051, 1082, 1136, 1156
HpaII CCGG 3 cut(s) 276, 306, 782
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 8 cut(s) 142, 217, 262, 346, 502, 509, 643, 879
Hpy188I TCNGA 2 cut(s) 398, 737
Hpy188III TCNNGA 7 cut(s) 124, 247, 539, 923, 956, 1133, 1140
Hpy8I GTNNAC 8 cut(s) 142, 217, 262, 346, 502, 509, 643, 879
Hpy99I CGWCG 2 cut(s) 248, 266
HpyAV CCTTC 6 cut(s) 39, 219, 278, 467, 545, 658
HpyCH4III ACNGT 5 cut(s) 203, 394, 506, 598, 894
HpyCH4IV ACGT 4 cut(s) 264, 342, 1020, 1115
HpyCH4V TGCA 6 cut(s) 162, 402, 602, 950, 1068, 1089
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 326, 677, 1065
HpyF3I CTNAG 3 cut(s) 681, 957, 1080
HpySE526I ACGT 4 cut(s) 264, 342, 1020, 1115
Hsp92I GRCGYC 1 cut(s) 264
Hsp92II CATG 3 cut(s) 583, 1089, 1136
KpnI GGTACC 1 cut(s) 1106
Kzo9I GATC 5 cut(s) 529, 559, 652, 843, 1142
LguI GCTCTTC 1 cut(s) 155
LmnI GCTCC 1 cut(s) 278
Lsp1109I GCAGC 3 cut(s) 116, 174, 934
LweI GCATC 4 cut(s) 389, 579, 916, 996
MaeI CTAG 2 cut(s) 471, 1140
MaeII ACGT 4 cut(s) 264, 342, 1020, 1115
MaeIII GTNAC 6 cut(s) 121, 203, 224, 284, 429, 473
MalI GATC 5 cut(s) 531, 561, 654, 845, 1144
MboI GATC 5 cut(s) 529, 559, 652, 843, 1142
MboII GAAGA 6 cut(s) 17, 142, 416, 724, 762, 1009
MluCI AATT 6 cut(s) 309, 834, 867, 1000, 1025, 1146
MlyI GAGTC 3 cut(s) 141, 504, 1091
MmeI TCCRAC 2 cut(s) 376, 809
MnlI CCTC 9 cut(s) 29, 124, 192, 244, 251, 481, 489, 508, 857
MroXI GAANNNNTTC 1 cut(s) 420
MseI TTAA 2 cut(s) 660, 1168
MslI CAYNNNNRTG 1 cut(s) 884
MspCI CTTAAG 1 cut(s) 659
MspI CCGG 3 cut(s) 276, 306, 782
MspR9I CCNGG 3 cut(s) 167, 567, 825
Mva1269I GAATGC 1 cut(s) 164
MvaI CCWGG 3 cut(s) 167, 567, 825
MwoI GCNNNNNNNGC 4 cut(s) 110, 326, 677, 1065
NcoI CCATGG 1 cut(s) 579
NdeII GATC 5 cut(s) 529, 559, 652, 843, 1142
NlaIII CATG 3 cut(s) 583, 1089, 1136
NlaIV GGNNCC 2 cut(s) 298, 1104
NmuCI GTSAC 3 cut(s) 121, 203, 429
PaeR7I CTCGAG 1 cut(s) 512
PagI TCATGA 1 cut(s) 1132
PceI AGGCCT 1 cut(s) 469
PciSI GCTCTTC 1 cut(s) 155
PcsI WCGNNNNNNNCGW 3 cut(s) 252, 258, 339
PctI GAATGC 1 cut(s) 164
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 3 cut(s) 1051, 1136, 1156
PkrI GCNGC 4 cut(s) 106, 164, 304, 949
PleI GAGTC 3 cut(s) 140, 504, 1090
PpsI GAGTC 3 cut(s) 140, 504, 1090
Ppu21I YACGTR 1 cut(s) 343
Psp6I CCWGG 3 cut(s) 165, 565, 823
PspEI GGTNACC 1 cut(s) 203
PspFI CCCAGC 2 cut(s) 316, 688
PspGI CCWGG 3 cut(s) 165, 565, 823
PspN4I GGNNCC 2 cut(s) 298, 1104
PspPI GGNCC 2 cut(s) 296, 517
PspXI VCTCGAGB 1 cut(s) 512
PstNI CAGNNNCTG 1 cut(s) 433
RsaI GTAC 2 cut(s) 891, 1104
RsaNI GTAC 2 cut(s) 890, 1103
RseI CAYNNNNRTG 1 cut(s) 884
SalI GTCGAC 1 cut(s) 260
SapI GCTCTTC 1 cut(s) 155
SaqAI TTAA 2 cut(s) 660, 1168
SatI GCNGC 4 cut(s) 105, 163, 303, 948
Sau3AI GATC 5 cut(s) 529, 559, 652, 843, 1142
Sau96I GGNCC 2 cut(s) 296, 517
SchI GAGTC 3 cut(s) 141, 504, 1091
ScrFI CCNGG 3 cut(s) 167, 567, 825
SfaNI GCATC 4 cut(s) 389, 579, 916, 996
SfcI CTRYAG 1 cut(s) 502
Sfr274I CTCGAG 1 cut(s) 512
SinI GGWCC 1 cut(s) 517
SlaI CTCGAG 1 cut(s) 512
SmiMI CAYNNNNRTG 1 cut(s) 884
SmlI CTYRAG 4 cut(s) 512, 659, 872, 1043
SmoI CTYRAG 4 cut(s) 512, 659, 872, 1043
Sse9I AATT 6 cut(s) 309, 834, 867, 1000, 1025, 1146
SseBI AGGCCT 1 cut(s) 469
SsiI CCGC 7 cut(s) 229, 300, 303, 329, 520, 671, 798
SspMI CTAG 2 cut(s) 471, 1140
StuI AGGCCT 1 cut(s) 469
StyD4I CCNGG 3 cut(s) 165, 565, 823
StyI CCWWGG 3 cut(s) 406, 464, 579
TaaI ACNGT 5 cut(s) 203, 394, 506, 598, 894
TaiI ACGT 4 cut(s) 267, 345, 1023, 1118
TasI AATT 6 cut(s) 309, 834, 867, 1000, 1025, 1146
TauI GCSGC 1 cut(s) 305
TfiI GAWTC 3 cut(s) 1051, 1136, 1156
Tru1I TTAA 2 cut(s) 660, 1168
Tru9I TTAA 2 cut(s) 660, 1168
TscAI CASTG 2 cut(s) 438, 511
TseFI GTSAC 3 cut(s) 121, 203, 429
TseI GCWGC 3 cut(s) 104, 162, 947
Tsp45I GTSAC 3 cut(s) 121, 203, 429
TspDTI ATGAA 5 cut(s) 17, 75, 171, 376, 1121
TspRI CASTG 2 cut(s) 438, 511
Vha464I CTTAAG 1 cut(s) 659
VpaK11BI GGWCC 1 cut(s) 517
XapI RAATTY 3 cut(s) 309, 867, 1000
XbaI TCTAGA 1 cut(s) 1139
XcmI CCANNNNNNNNNTGG 1 cut(s) 546
XhoI CTCGAG 1 cut(s) 512
XmiI GTMKAC 4 cut(s) 141, 216, 261, 501
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 2 cut(s) 471, 1140
ZraI GACGTC 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.