Rh2CG313600

Aminotransferase class I and II

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0004272
Physical Location & Seq
Forward (+)
39648962 .. 39652267
3306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG313600.1

Sequence Viewer

Length: 1260 bp
ATGGAAATGGAAAACAAGTGGAATTTTCAGGGGAATGGAGAGCTCAAAACGGAACCACCAACCACGGTTCGAGGAGTTCTCACCATGATAATGCAAAATCTCAACAAAGATGATCCAAGGTCTATCATTCCTTTAGGCCACGGTGACCCCTCCGCTTTCCCATGCTTTCGGACTAGTATTTCAGCCGAAGATGCCATCGTTGATTCTGTTCGGTCCGGCAAGTTTAACTGCTATTCTCCCGCAGTTGGTATTCTACCAGCAAGGAGGGCTATTGCAGACTATCTGTCAAATGATCTCCCATACAAGTTATCGCCTGACGATGTTTATCTTACAATTGGGTGTACACAAGCAATTGAAGTGGTACTGACAGTTATATCCCTGTCAAATCCTGTTGCCAACATTCTGCTTCCAAGGCCATGCTTCCCTTTCTATGAAACTCGTGCAGCCTGTTCCAATCTGGAAGTTCGTCGCTATGATCTGCTTCCGGAACAGGGTTGGGAGGTTGATCTTGAAGCTCTTGAAGCACTTGCAGATGAAAATACTGTTGCCATGGTCATCATCAATCCTGGAAATCCTTGTGGAAATGTTTATACGTCTCAACATTTGAAGAAGATTGCAGAGACGGCAAGGAAGCTTGGGATTCTTGTAATTGCTGATGAAGTGTATGACCATCTTACTTTCGCAAATACCCCATTTGTGCCAATGGGGACATTTGGATCTGTTGTCCCAGTTCTTACACTTGGTTCTATATCAAAGAGATGGATTGTACCTGGTTGGAGACTTGGTTGGATTGTTACCTGTGACCCCAACGCAATTCTTCAAAAGTCGGGAATTATGAAGTCCATTACAGGATGTCTCGAAGTCTCATCGGATGCTCCAACCTTCATTCAGGCAGCAATTCCTCAAATTATAGAGAATACAAAGGAGGATTTCTTTCTGAAAATAGTTGAGATGCTACGAGGTGCTGCAGACATATGTTTTGATAAACTTAATGAGATTCCCTGCATTACGTGCCCAAGTAAACCTGAGGGTTCCATGTTTGTAATGGTAAAGCTGAATCTCTCATTGTTCGATGACATTAGTGATGATGTTGAGTTCAGCCTCAAACTTGCCAAAGAGGAATCTGTCATAGTACTACCTGGTGTGACTGTTGGATTGAAGAACTGGTTGCGGATAACATTTGCTTGTGACCATTCAATTCTTGAAGATGGCCTTGGGAGGATTAAAGGCTTCTGTGGAAGGCATGCCAAGAAACAATAA

Protein Analysis

419

Amino Acids

46.14

Weight (kDa)

5.1

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 42 - 407 1.1e-52 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 484
AciI CCGC 3 cut(s) 153, 240, 1171
AclWI GGATC 2 cut(s) 107, 724
AcsI RAATTY 1 cut(s) 22
AfaI GTAC 4 cut(s) 343, 363, 768, 1134
AfiI CCNNNNNNNGG 2 cut(s) 245, 491
AgsI TTSAA 8 cut(s) 356, 512, 521, 607, 821, 1159, 1197, 1205
AhdI GACNNNNNGTC 1 cut(s) 283
AhlI ACTAGT 1 cut(s) 173
AjnI CCWGG 3 cut(s) 565, 769, 1138
AjuI GAANNNNNNNTTGG 2 cut(s) 1197, 1229
AluBI AGCT 4 cut(s) 43, 515, 634, 1054
AluI AGCT 4 cut(s) 43, 515, 634, 1054
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 5 cut(s) 600, 614, 772, 860, 868
AlwI GGATC 2 cut(s) 107, 724
Aor13HI TCCGGA 1 cut(s) 484
AoxI GGCC 3 cut(s) 136, 413, 1210
ApeKI GCWGC 3 cut(s) 443, 893, 965
ApoI RAATTY 1 cut(s) 22
ArsI GACNNNNNNTTYG 2 cut(s) 962, 994
AspS9I GGNCC 1 cut(s) 213
AsuHPI GGTGA 2 cut(s) 73, 155
AvaII GGWCC 1 cut(s) 213
AxyI CCTNAGG 1 cut(s) 1026
BaeGI GKGCMC 1 cut(s) 1016
BanII GRGCYC 1 cut(s) 45
BauI CACGAG 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 3 cut(s) 455, 905, 952
BccI CCATC 4 cut(s) 203, 678, 753, 1202
BceAI ACGGC 1 cut(s) 639
BciT130I CCWGG 3 cut(s) 567, 771, 1140
BcoDI GTCTC 5 cut(s) 600, 614, 772, 860, 868
BcuI ACTAGT 1 cut(s) 173
BfaI CTAG 1 cut(s) 174
BfmI CTRYAG 1 cut(s) 966
BisI GCNGC 3 cut(s) 444, 894, 966
BlsI GCNGC 3 cut(s) 445, 895, 967
BmcAI AGTACT 1 cut(s) 1134
Bme1390I CCNGG 3 cut(s) 567, 771, 1140
Bme18I GGWCC 1 cut(s) 213
BmeRI GACNNNNNGTC 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 213
BmiI GGNNCC 2 cut(s) 54, 1033
BmrFI CCNGG 3 cut(s) 567, 771, 1140
BmrI ACTGGG 1 cut(s) 722
BmsI GCATC 3 cut(s) 181, 862, 942
BmuI ACTGGG 1 cut(s) 722
BplI GAGNNNNNCTC 2 cut(s) 63, 95
BsaAI YACGTR 1 cut(s) 1011
BsaBI GATNNNNATC 1 cut(s) 324
BsaJI CCNNGG 6 cut(s) 63, 116, 139, 410, 549, 1213
BsaWI WCCGGW 1 cut(s) 484
BsaXI ACNNNNNCTCC 2 cut(s) 769, 799
Bsc4I CCNNNNNNNGG 2 cut(s) 245, 491
Bse1I ACTGG 2 cut(s) 728, 1169
Bse21I CCTNAGG 1 cut(s) 1026
Bse8I GATNNNNATC 1 cut(s) 324
BseAI TCCGGA 1 cut(s) 484
BseBI CCWGG 3 cut(s) 567, 771, 1140
BseDI CCNNGG 6 cut(s) 63, 116, 139, 410, 549, 1213
BseGI GGATG 2 cut(s) 857, 877
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 2 cut(s) 245, 491
BseMII CTCAG 1 cut(s) 1017
BseNI ACTGG 2 cut(s) 728, 1169
BseRI GAGGAG 1 cut(s) 87
BseSI GKGCMC 1 cut(s) 1016
BseXI GCAGC 3 cut(s) 455, 905, 952
BsgI GTGCAG 1 cut(s) 462
BshFI GGCC 3 cut(s) 138, 415, 1212
BsiHKAI GWGCWC 1 cut(s) 45
BsiSI CCGG 2 cut(s) 216, 485
BslFI GGGAC 2 cut(s) 710, 721
BslI CCNNNNNNNGG 2 cut(s) 245, 491
BsmAI GTCTC 5 cut(s) 600, 614, 772, 860, 868
BsmBI CGTCTC 2 cut(s) 600, 614
BsmFI GGGAC 2 cut(s) 710, 721
BsnI GGCC 3 cut(s) 138, 415, 1212
Bsp1286I GDGCHC 2 cut(s) 45, 1016
Bsp13I TCCGGA 1 cut(s) 484
Bsp1407I TGTACA 1 cut(s) 341
Bsp143I GATC 5 cut(s) 112, 292, 475, 505, 716
Bsp19I CCATGG 1 cut(s) 549
BspACI CCGC 3 cut(s) 153, 240, 1171
BspANI GGCC 3 cut(s) 138, 415, 1212
BspCNI CTCAG 1 cut(s) 1018
BspEI TCCGGA 1 cut(s) 484
BspLI GGNNCC 2 cut(s) 54, 1033
BspMAI CTGCAG 1 cut(s) 970
BspPI GGATC 2 cut(s) 107, 724
BsrGI TGTACA 1 cut(s) 341
BsrI ACTGG 2 cut(s) 728, 1169
BssECI CCNNGG 6 cut(s) 63, 116, 139, 410, 549, 1213
BssMI GATC 5 cut(s) 112, 292, 475, 505, 716
BssSI CACGAG 1 cut(s) 438
BssT1I CCWWGG 4 cut(s) 116, 410, 549, 1213
Bst2BI CACGAG 1 cut(s) 438
Bst2UI CCWGG 3 cut(s) 567, 771, 1140
Bst4CI ACNGT 5 cut(s) 67, 143, 370, 544, 1150
BstAPI GCANNNNNTGC 1 cut(s) 1011
BstAUI TGTACA 1 cut(s) 341
BstBAI YACGTR 1 cut(s) 1011
BstC8I GCNNGC 1 cut(s) 1245
BstDEI CTNAG 1 cut(s) 1026
BstDSI CCRYGG 3 cut(s) 63, 139, 549
BstEII GGTNACC 1 cut(s) 143
BstF5I GGATG 2 cut(s) 857, 877
BstKTI GATC 5 cut(s) 115, 295, 478, 508, 719
BstMAI GTCTC 5 cut(s) 600, 614, 772, 860, 868
BstMBI GATC 5 cut(s) 112, 292, 475, 505, 716
BstMWI GCNNNNNNNGC 6 cut(s) 191, 266, 412, 521, 623, 1011
BstNI CCWGG 3 cut(s) 567, 771, 1140
BstNSI RCATGY 1 cut(s) 1247
BstPI GGTNACC 1 cut(s) 143
BstSCI CCNGG 3 cut(s) 565, 769, 1138
BstSFI CTRYAG 1 cut(s) 966
BstSLI GKGCMC 1 cut(s) 1016
BstV1I GCAGC 3 cut(s) 455, 905, 952
BstX2I RGATCY 1 cut(s) 716
BstYI RGATCY 1 cut(s) 716
Bsu36I CCTNAGG 1 cut(s) 1026
BsuRI GGCC 3 cut(s) 138, 415, 1212
BtgI CCRYGG 3 cut(s) 63, 139, 549
BtsCI GGATG 2 cut(s) 857, 877
Cac8I GCNNGC 1 cut(s) 1245
Cfr13I GGNCC 1 cut(s) 213
CpoI CGGWCCG 1 cut(s) 213
CsiI ACCWGGT 2 cut(s) 769, 1138
Csp6I GTAC 4 cut(s) 342, 362, 767, 1133
CspCI CAANNNNNGTGG 2 cut(s) 339, 374
CspI CGGWCCG 1 cut(s) 213
CviAII CATG 6 cut(s) 85, 162, 417, 550, 1036, 1244
CviQI GTAC 4 cut(s) 342, 362, 767, 1133
DdeI CTNAG 1 cut(s) 1026
DpnI GATC 5 cut(s) 114, 294, 477, 507, 718
DpnII GATC 5 cut(s) 112, 292, 475, 505, 716
DriI GACNNNNNGTC 1 cut(s) 283
Eam1105I GACNNNNNGTC 1 cut(s) 283
Ecl136II GAGCTC 1 cut(s) 43
Eco130I CCWWGG 4 cut(s) 116, 410, 549, 1213
Eco24I GRGCYC 1 cut(s) 45
Eco47I GGWCC 1 cut(s) 213
Eco53kI GAGCTC 1 cut(s) 43
Eco81I CCTNAGG 1 cut(s) 1026
Eco91I GGTNACC 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 43
EcoO65I GGTNACC 1 cut(s) 143
EcoRII CCWGG 3 cut(s) 565, 769, 1138
EcoT14I CCWWGG 4 cut(s) 116, 410, 549, 1213
EcoT38I GRGCYC 1 cut(s) 45
ErhI CCWWGG 4 cut(s) 116, 410, 549, 1213
Esp3I CGTCTC 2 cut(s) 600, 614
FaeI CATG 6 cut(s) 88, 165, 420, 553, 1039, 1247
FalI AAGNNNNNCTT 2 cut(s) 1197, 1229
FaqI GGGAC 2 cut(s) 710, 721
FatI CATG 6 cut(s) 84, 161, 416, 549, 1035, 1243
FauI CCCGC 1 cut(s) 247
FauNDI CATATG 1 cut(s) 974
Fnu4HI GCNGC 3 cut(s) 444, 894, 966
FokI GGATG 2 cut(s) 864, 884
FriOI GRGCYC 1 cut(s) 45
Fsp4HI GCNGC 3 cut(s) 444, 894, 966
FspBI CTAG 1 cut(s) 174
GluI GCNGC 3 cut(s) 444, 894, 966
HaeIII GGCC 3 cut(s) 138, 415, 1212
HapII CCGG 2 cut(s) 216, 485
Hin1II CATG 6 cut(s) 88, 165, 420, 553, 1039, 1247
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 5 cut(s) 203, 640, 997, 1057, 1121
HpaII CCGG 2 cut(s) 216, 485
HphI GGTGA 2 cut(s) 73, 155
Hpy166II GTNNAC 3 cut(s) 342, 344, 1022
Hpy188I TCNGA 3 cut(s) 171, 871, 939
Hpy188III TCNNGA 7 cut(s) 458, 485, 509, 518, 828, 857, 1202
Hpy8I GTNNAC 3 cut(s) 342, 344, 1022
Hpy99I CGWCG 1 cut(s) 471
HpyAV CCTTC 2 cut(s) 892, 1233
HpyCH4III ACNGT 5 cut(s) 67, 143, 370, 544, 1150
HpyCH4IV ACGT 2 cut(s) 593, 1010
HpyCH4V TGCA 7 cut(s) 94, 275, 443, 530, 617, 968, 1005
HpyF10VI GCNNNNNNNGC 6 cut(s) 191, 266, 412, 521, 623, 1011
HpyF3I CTNAG 1 cut(s) 1026
HpySE526I ACGT 2 cut(s) 593, 1010
Hsp92II CATG 6 cut(s) 88, 165, 420, 553, 1039, 1247
Kpn2I TCCGGA 1 cut(s) 484
Kzo9I GATC 5 cut(s) 112, 292, 475, 505, 716
LmnI GCTCC 1 cut(s) 880
Lsp1109I GCAGC 3 cut(s) 455, 905, 952
LweI GCATC 3 cut(s) 181, 862, 942
MabI ACCWGGT 2 cut(s) 769, 1138
MaeI CTAG 1 cut(s) 174
MaeII ACGT 2 cut(s) 593, 1010
MaeIII GTNAC 5 cut(s) 143, 793, 800, 1144, 1187
MalI GATC 5 cut(s) 114, 294, 477, 507, 718
MboI GATC 5 cut(s) 112, 292, 475, 505, 716
MboII GAAGA 6 cut(s) 200, 619, 622, 809, 1171, 1217
MfeI CAATTG 2 cut(s) 333, 351
MflI RGATCY 1 cut(s) 716
MhlI GDGCHC 2 cut(s) 45, 1016
MluCI AATT 9 cut(s) 22, 333, 351, 648, 813, 831, 897, 906, 1197
MmeI TCCRAC 4 cut(s) 755, 767, 902, 1132
MroI TCCGGA 1 cut(s) 484
MseI TTAA 3 cut(s) 225, 990, 1224
MslI CAYNNNNRTG 1 cut(s) 89
MspI CCGG 2 cut(s) 216, 485
MspR9I CCNGG 3 cut(s) 567, 771, 1140
MunI CAATTG 2 cut(s) 333, 351
MvaI CCWGG 3 cut(s) 567, 771, 1140
MwoI GCNNNNNNNGC 6 cut(s) 191, 266, 412, 521, 623, 1011
NcoI CCATGG 1 cut(s) 549
NdeI CATATG 1 cut(s) 974
NdeII GATC 5 cut(s) 112, 292, 475, 505, 716
NlaIII CATG 6 cut(s) 88, 165, 420, 553, 1039, 1247
NlaIV GGNNCC 2 cut(s) 54, 1033
NmuCI GTSAC 4 cut(s) 143, 800, 1144, 1187
NspI RCATGY 1 cut(s) 1247
PaeI GCATGC 1 cut(s) 1247
PfeI GAWTC 5 cut(s) 203, 640, 997, 1057, 1121
PfoI TCCNGGA 1 cut(s) 565
PkrI GCNGC 3 cut(s) 445, 895, 967
Ppu21I YACGTR 1 cut(s) 1011
Psp124BI GAGCTC 1 cut(s) 45
Psp6I CCWGG 3 cut(s) 565, 769, 1138
PspEI GGTNACC 1 cut(s) 143
PspGI CCWGG 3 cut(s) 565, 769, 1138
PspN4I GGNNCC 2 cut(s) 54, 1033
PspPI GGNCC 1 cut(s) 213
PstI CTGCAG 1 cut(s) 970
PsuI RGATCY 1 cut(s) 716
RsaI GTAC 4 cut(s) 343, 363, 768, 1134
RsaNI GTAC 4 cut(s) 342, 362, 767, 1133
RseI CAYNNNNRTG 1 cut(s) 89
Rsr2I CGGWCCG 1 cut(s) 213
RsrII CGGWCCG 1 cut(s) 213
SacI GAGCTC 1 cut(s) 45
SaqAI TTAA 3 cut(s) 225, 990, 1224
SatI GCNGC 3 cut(s) 444, 894, 966
Sau3AI GATC 5 cut(s) 112, 292, 475, 505, 716
Sau96I GGNCC 1 cut(s) 213
ScaI AGTACT 1 cut(s) 1134
ScrFI CCNGG 3 cut(s) 567, 771, 1140
SduI GDGCHC 2 cut(s) 45, 1016
SexAI ACCWGGT 2 cut(s) 769, 1138
SfaNI GCATC 3 cut(s) 181, 862, 942
SfcI CTRYAG 1 cut(s) 966
SinI GGWCC 1 cut(s) 213
SmiMI CAYNNNNRTG 1 cut(s) 89
SpeI ACTAGT 1 cut(s) 173
SphI GCATGC 1 cut(s) 1247
Sse9I AATT 9 cut(s) 22, 333, 351, 648, 813, 831, 897, 906, 1197
SsiI CCGC 3 cut(s) 153, 240, 1171
SspMI CTAG 1 cut(s) 174
SstI GAGCTC 1 cut(s) 45
StyD4I CCNGG 3 cut(s) 565, 769, 1138
StyI CCWWGG 4 cut(s) 116, 410, 549, 1213
TaaI ACNGT 5 cut(s) 67, 143, 370, 544, 1150
TaiI ACGT 2 cut(s) 596, 1013
TaqI TCGA 3 cut(s) 70, 858, 1071
TaqII GACCGA 1 cut(s) 201
TasI AATT 9 cut(s) 22, 333, 351, 648, 813, 831, 897, 906, 1197
TatI WGTACW 2 cut(s) 341, 1132
TfiI GAWTC 5 cut(s) 203, 640, 997, 1057, 1121
Tru1I TTAA 3 cut(s) 225, 990, 1224
Tru9I TTAA 3 cut(s) 225, 990, 1224
TseFI GTSAC 4 cut(s) 143, 800, 1144, 1187
TseI GCWGC 3 cut(s) 443, 893, 965
Tsp45I GTSAC 4 cut(s) 143, 800, 1144, 1187
TspDTI ATGAA 5 cut(s) 447, 549, 672, 851, 874
TspGWI ACGGA 1 cut(s) 65
VpaK11BI GGWCC 1 cut(s) 213
XapI RAATTY 1 cut(s) 22
XceI RCATGY 1 cut(s) 1247
XcmI CCANNNNNNNNNTGG 1 cut(s) 1042
XspI CTAG 1 cut(s) 174
ZrmI AGTACT 1 cut(s) 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.