AT4G33350

Tic22-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012495
Physical Location & Seq
Reverse (-)
16064437 .. 16066897
2461 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G33350.1

Sequence Viewer

Length: 807 bp
ATGGAGTCATCAGTGAAACCCAATCCTTTCCTCTCCTTCTCTTCTTTCATTCACCACCAGTGTACCCGCTTCAGCTCCGACCTCTCCGCCCGAATCGAAGACACGAAACGATTCGCCGAAACCCTAGCTACTCGACGTTTCTCTCTTCCTACTCCGCCGCCTTTCGCCTCCGTCTCACAATCCAAGTCTGGAACCCCGACGACGACGTTGAGTCCATCTCTTGTCGCTAAAGCTCTCGCTGGGACGTCGGTGTTTACAGTCAGTAACACCAATAACGAATTCGTTCTTATCTCGGATCCTACCGGAGGCAAGTCTATCGGTTTGCTCTGTTTTCGACAAGAGGATGCTGAAGCTTTTCTTGCTCAGGCGCGACTTCGGAGAAGAGAATTGAAAACCAATGCGAAGGTTGTTCCCATTACTCTGGACCAGGTGTACTTGCTAAAAGTTGAAGGGATTTCCTTTCGCTTCTTGCCAGATCCAATCCAAATAAAGAATGCGTTAGAGCTCAAATCCTCAGGAAATAAGAATGGGTTTGATGGAGTTCCGGTTTTTCAGTCTGAGCTTCTTGTTGTGAGGAAAAAAAATAGACGTTACTGCCCTGTATATTTCAGTAAGGAAGATATAGAGAGGGAACTTTCTAAATATACGAGAGCATCAAGAGGAGATCAACAAATTATGGTTGGGAGTCTGGAAGATGTGTTGAGGAAAATGGAGATGAGCGAGAAGAATTCAGGTTGGGAAGATGTAATATTCATTCCACCGGGAAGAAGTTATGCACAACACATGCAGGATTTGATCAAAGAGTAA

Protein Analysis

268

Amino Acids

30.11

Weight (kDa)

9.32

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 10 - 264 6.8e-113 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33350 AT4G33350
fragaria_vesca FvH4_2g34490
malus_domestica MD08G1102400.v1.1 MD15G1083800.v1.1
prunus_persica Prupe.1G436300_v2.0.a1
pyrus_communis pycom08g08320 pycom15g07850 pycom15g07890
rosa_chinensis RchiOBHm_Chr6g0307851
rosa_laevigata RLG00000010693
rosa_multiflora Rmu_sc0003252.1_g000014
rosa_roxburghii Rroxscaffold_7G00160820
rosa_rugosa Rorug06G0361400
rosa_samantha Rh6AG470200 Rh6BG481200 Rh6CG486500 Rh6DG472700
rosa_wichuraiana Rw6G041050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 248
AccII CGCG 1 cut(s) 370
AciI CCGC 4 cut(s) 67, 87, 155, 158
AclWI GGATC 3 cut(s) 290, 303, 470
AcsI RAATTY 2 cut(s) 278, 727
AcuI CTGAAG 2 cut(s) 55, 369
AcyI GRCGYC 1 cut(s) 245
AfaI GTAC 2 cut(s) 64, 434
AfiI CCNNNNNNNGG 1 cut(s) 305
AgsI TTSAA 2 cut(s) 391, 449
AhdI GACNNNNNGTC 1 cut(s) 210
AjnI CCWGG 1 cut(s) 426
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AluBI AGCT 6 cut(s) 75, 128, 233, 353, 505, 562
AluI AGCT 6 cut(s) 75, 128, 233, 353, 505, 562
Alw21I GWGCWC 1 cut(s) 507
Alw26I GTCTC 1 cut(s) 178
AlwI GGATC 3 cut(s) 290, 303, 470
ApoI RAATTY 2 cut(s) 278, 727
Asp700I GAANNNNTTC 3 cut(s) 110, 282, 354
AspLEI GCGC 1 cut(s) 370
AspS9I GGNCC 1 cut(s) 424
AsuC2I CCSGG 1 cut(s) 762
AsuHPI GGTGA 1 cut(s) 44
AvaII GGWCC 1 cut(s) 424
AxyI CCTNAGG 1 cut(s) 514
BaeI ACNNNNGTAYC 2 cut(s) 46, 79
BamHI GGATCC 1 cut(s) 295
BanII GRGCYC 1 cut(s) 507
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 507
BccI CCATC 2 cut(s) 223, 530
BcgI CGANNNNNNTGC 2 cut(s) 298, 332
BciT130I CCWGG 1 cut(s) 428
BclI TGATCA 1 cut(s) 795
BcnI CCSGG 1 cut(s) 762
BcoDI GTCTC 1 cut(s) 178
BfaI CTAG 1 cut(s) 125
BisI GCNGC 1 cut(s) 158
BlsI GCNGC 1 cut(s) 159
Bme1390I CCNGG 2 cut(s) 428, 762
Bme18I GGWCC 1 cut(s) 424
BmeRI GACNNNNNGTC 1 cut(s) 210
BmgT120I GGNCC 1 cut(s) 424
BmiI GGNNCC 2 cut(s) 193, 297
BmrFI CCNGG 2 cut(s) 428, 762
BmsI GCATC 2 cut(s) 334, 662
BpiI GAAGAC 1 cut(s) 105
BplI GAGNNNNNCTC 2 cut(s) 202, 234
Bpu10I CCTNAGC 1 cut(s) 363
BpuMI CCSGG 1 cut(s) 762
BsaHI GRCGYC 1 cut(s) 245
BsaWI WCCGGW 2 cut(s) 302, 544
BsaXI ACNNNNNCTCC 2 cut(s) 531, 561
Bsc4I CCNNNNNNNGG 1 cut(s) 305
Bse1I ACTGG 1 cut(s) 58
Bse21I CCTNAGG 1 cut(s) 514
BseBI CCWGG 1 cut(s) 428
BseGI GGATG 1 cut(s) 349
BseLI CCNNNNNNNGG 1 cut(s) 305
BseMII CTCAG 3 cut(s) 377, 528, 549
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 1 cut(s) 675
BseYI CCCAGC 1 cut(s) 239
Bsh1236I CGCG 1 cut(s) 370
BsiHKAI GWGCWC 1 cut(s) 507
BsiSI CCGG 3 cut(s) 303, 545, 761
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 1 cut(s) 305
BsmAI GTCTC 1 cut(s) 178
BsmBI CGTCTC 1 cut(s) 178
BsmFI GGGAC 1 cut(s) 256
BsmI GAATGC 1 cut(s) 499
Bsp1286I GDGCHC 1 cut(s) 507
Bsp143I GATC 4 cut(s) 295, 475, 664, 795
BspACI CCGC 4 cut(s) 67, 87, 155, 158
BspCNI CTCAG 3 cut(s) 376, 527, 550
BspFNI CGCG 1 cut(s) 370
BspLI GGNNCC 2 cut(s) 193, 297
BspPI GGATC 3 cut(s) 290, 303, 470
BsrI ACTGG 1 cut(s) 58
BssMI GATC 4 cut(s) 295, 475, 664, 795
BssNI GRCGYC 1 cut(s) 245
Bst2UI CCWGG 1 cut(s) 428
Bst4CI ACNGT 1 cut(s) 259
Bst6I CTCTTC 3 cut(s) 46, 150, 376
BstACI GRCGYC 1 cut(s) 245
BstDEI CTNAG 3 cut(s) 363, 514, 558
BstENI CCTNNNNNAGG 1 cut(s) 303
BstF5I GGATG 1 cut(s) 349
BstFNI CGCG 1 cut(s) 370
BstHHI GCGC 1 cut(s) 370
BstKTI GATC 4 cut(s) 298, 478, 667, 798
BstMAI GTCTC 1 cut(s) 178
BstMBI GATC 4 cut(s) 295, 475, 664, 795
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstNI CCWGG 1 cut(s) 428
BstNSI RCATGY 1 cut(s) 787
BstSCI CCNGG 2 cut(s) 426, 760
BstUI CGCG 1 cut(s) 370
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 2 cut(s) 295, 475
BstXI CCANNNNNNTGG 1 cut(s) 421
BstYI RGATCY 2 cut(s) 295, 475
Bsu36I CCTNAGG 1 cut(s) 514
BtsCI GGATG 1 cut(s) 349
BtsIMutI CAGTG 2 cut(s) 18, 65
CfoI GCGC 1 cut(s) 370
Cfr13I GGNCC 1 cut(s) 424
CsiI ACCWGGT 1 cut(s) 426
Csp6I GTAC 2 cut(s) 63, 433
CviAII CATG 1 cut(s) 784
CviJI RGCY 6 cut(s) 75, 128, 233, 353, 505, 562
CviKI_1 RGCY 6 cut(s) 75, 128, 233, 353, 505, 562
CviQI GTAC 2 cut(s) 63, 433
DdeI CTNAG 3 cut(s) 363, 514, 558
DpnI GATC 4 cut(s) 297, 477, 666, 797
DpnII GATC 4 cut(s) 295, 475, 664, 795
DriI GACNNNNNGTC 1 cut(s) 210
Eam1104I CTCTTC 3 cut(s) 46, 150, 376
Eam1105I GACNNNNNGTC 1 cut(s) 210
EarI CTCTTC 3 cut(s) 46, 150, 376
EciI GGCGGA 2 cut(s) 76, 144
Ecl136II GAGCTC 1 cut(s) 505
Eco24I GRGCYC 1 cut(s) 507
Eco47I GGWCC 1 cut(s) 424
Eco53kI GAGCTC 1 cut(s) 505
Eco57I CTGAAG 2 cut(s) 55, 369
Eco81I CCTNAGG 1 cut(s) 514
EcoICRI GAGCTC 1 cut(s) 505
EcoNI CCTNNNNNAGG 1 cut(s) 303
EcoRI GAATTC 2 cut(s) 278, 727
EcoRII CCWGG 1 cut(s) 426
EcoT38I GRGCYC 1 cut(s) 507
Esp3I CGTCTC 1 cut(s) 178
FaeI CATG 1 cut(s) 787
FaiI YATR 6 cut(s) 604, 623, 645, 677, 774, 785
FalI AAGNNNNNCTT 2 cut(s) 342, 374
FaqI GGGAC 1 cut(s) 256
FatI CATG 1 cut(s) 783
FauI CCCGC 1 cut(s) 74
FbaI TGATCA 1 cut(s) 795
Fnu4HI GCNGC 1 cut(s) 158
FokI GGATG 1 cut(s) 356
FriOI GRGCYC 1 cut(s) 507
Fsp4HI GCNGC 1 cut(s) 158
FspBI CTAG 1 cut(s) 125
GlaI GCGC 1 cut(s) 369
GluI GCNGC 1 cut(s) 158
GsaI CCCAGC 1 cut(s) 243
HapII CCGG 3 cut(s) 303, 545, 761
HhaI GCGC 1 cut(s) 370
Hin1I GRCGYC 1 cut(s) 245
Hin1II CATG 1 cut(s) 787
Hin6I GCGC 1 cut(s) 368
HinP1I GCGC 1 cut(s) 368
HindIII AAGCTT 1 cut(s) 351
HinfI GANTC 5 cut(s) 5, 93, 111, 211, 685
HpaII CCGG 3 cut(s) 303, 545, 761
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 3 cut(s) 63, 255, 433
Hpy188I TCNGA 4 cut(s) 79, 295, 378, 559
Hpy188III TCNNGA 5 cut(s) 189, 422, 516, 657, 689
Hpy8I GTNNAC 3 cut(s) 63, 255, 433
Hpy99I CGWCG 5 cut(s) 138, 202, 205, 208, 250
HpyAV CCTTC 3 cut(s) 46, 397, 443
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4IV ACGT 4 cut(s) 136, 206, 245, 589
HpyCH4V TGCA 2 cut(s) 776, 787
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
HpyF3I CTNAG 3 cut(s) 363, 514, 558
HpySE526I ACGT 4 cut(s) 136, 206, 245, 589
Hsp92I GRCGYC 1 cut(s) 245
Hsp92II CATG 1 cut(s) 787
HspAI GCGC 1 cut(s) 368
Ksp22I TGATCA 1 cut(s) 795
Kzo9I GATC 4 cut(s) 295, 475, 664, 795
LmnI GCTCC 1 cut(s) 80
LweI GCATC 2 cut(s) 334, 662
MabI ACCWGGT 1 cut(s) 426
MaeI CTAG 1 cut(s) 125
MaeII ACGT 4 cut(s) 136, 206, 245, 589
MaeIII GTNAC 2 cut(s) 263, 590
MalI GATC 4 cut(s) 297, 477, 666, 797
MboI GATC 4 cut(s) 295, 475, 664, 795
MboII GAAGA 9 cut(s) 33, 110, 137, 393, 629, 704, 736, 752, 777
MflI RGATCY 2 cut(s) 295, 475
MhlI GDGCHC 1 cut(s) 507
MluCI AATT 4 cut(s) 278, 386, 672, 727
MlyI GAGTC 3 cut(s) 14, 220, 694
MmeI TCCRAC 1 cut(s) 102
MroXI GAANNNNTTC 3 cut(s) 110, 282, 354
MspI CCGG 3 cut(s) 303, 545, 761
MspR9I CCNGG 2 cut(s) 428, 762
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 1 cut(s) 428
MvnI CGCG 1 cut(s) 370
MwoI GCNNNNNNNGC 1 cut(s) 359
NciI CCSGG 1 cut(s) 762
NdeII GATC 4 cut(s) 295, 475, 664, 795
NlaIII CATG 1 cut(s) 787
NlaIV GGNNCC 2 cut(s) 193, 297
NspI RCATGY 1 cut(s) 787
PctI GAATGC 1 cut(s) 499
PdmI GAANNNNTTC 3 cut(s) 110, 282, 354
PfeI GAWTC 2 cut(s) 93, 111
PkrI GCNGC 1 cut(s) 159
PleI GAGTC 3 cut(s) 13, 219, 693
PpsI GAGTC 3 cut(s) 13, 219, 693
Psp124BI GAGCTC 1 cut(s) 507
Psp6I CCWGG 1 cut(s) 426
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 1 cut(s) 426
PspN4I GGNNCC 2 cut(s) 193, 297
PspPI GGNCC 1 cut(s) 424
PsuI RGATCY 2 cut(s) 295, 475
RsaI GTAC 2 cut(s) 64, 434
RsaNI GTAC 2 cut(s) 63, 433
SacI GAGCTC 1 cut(s) 507
SatI GCNGC 1 cut(s) 158
Sau3AI GATC 4 cut(s) 295, 475, 664, 795
Sau96I GGNCC 1 cut(s) 424
SchI GAGTC 3 cut(s) 14, 220, 694
ScrFI CCNGG 2 cut(s) 428, 762
SduI GDGCHC 1 cut(s) 507
SexAI ACCWGGT 1 cut(s) 426
SfaNI GCATC 2 cut(s) 334, 662
SinI GGWCC 1 cut(s) 424
Sse9I AATT 4 cut(s) 278, 386, 672, 727
SsiI CCGC 4 cut(s) 67, 87, 155, 158
SspI AATATT 1 cut(s) 750
SspMI CTAG 1 cut(s) 125
SstI GAGCTC 1 cut(s) 507
StyD4I CCNGG 2 cut(s) 426, 760
TaaI ACNGT 1 cut(s) 259
TaiI ACGT 4 cut(s) 139, 209, 248, 592
TaqI TCGA 3 cut(s) 96, 133, 334
TasI AATT 4 cut(s) 278, 386, 672, 727
TatI WGTACW 1 cut(s) 432
TauI GCSGC 1 cut(s) 160
TfiI GAWTC 2 cut(s) 93, 111
TscAI CASTG 2 cut(s) 18, 65
TspDTI ATGAA 2 cut(s) 37, 742
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 2 cut(s) 18, 65
VpaK11BI GGWCC 1 cut(s) 424
XagI CCTNNNNNAGG 1 cut(s) 303
XapI RAATTY 2 cut(s) 278, 727
XceI RCATGY 1 cut(s) 787
XmnI GAANNNNTTC 3 cut(s) 110, 282, 354
XspI CTAG 1 cut(s) 125
ZraI GACGTC 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.