MD08G1102400.v1.1

Protein TIC 22, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012495
Physical Location & Seq
Reverse (-)
8590572 .. 8593728
3157 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1102400.v1.1.491

Sequence Viewer

Length: 840 bp
ATGGAGTCCCCAAAGCCCTACCCGCGCTCAAACCCTCTGCTATCGCTCTCCACCTTCATACACCAGCACTGCCTCCGCCTCGGCGCCGAGCTCTCCACCCGCCTCGAAGACACCAAGCGGCTCGCCGGAACCATAGCAGGCAACTTCACGCCGGCGGCGAGCAGGCGCATGCGCTCGGCGCCGGTTTCTCCTCCCTTCGCGTGGCTGGCTCAGAGTAATCACGCTCTGGCCGCTACCCTCAGCTCTGACCACGTGGCGAAGAGCCTGGCCGGGACGGCCGTATATACGGTTAGCAATTCGAACAATGAGTTCGTGCTCATCTCTGATCCCAATGAGGCCAAGTCCATTGGATTGCTTTGCTTTCGGCACGAAGATGCCGAAGCATTCCTCGCTCAAGTTCGATCGAGGAGAGAATTACGAAGTGCGGCTAAAGTTGTCCCGATTACTCTTGACCAGGTGTACATGCTGAAGGTTGAAGGAATTGCATTCAGGTTTCTGCCTGACCCAGTTCAAATAAGGAATGCACTTGAGCTCAAATCCGCTGACAGGAGTGCATTTGATGGAGTTCCTGTTTTTCAGTCCGACCTTCTGGTTATGAAGAAGAAAAACAAGCGTTACTGCCCAATATATTTCACCAAGGAAGATATTGAAAAAGAATTGTCAAAGGTATCCAGAGCATCCAGAGGACCAGGGGTTTCTCAACATATAATGGTTGGCAGTTTGGAAGATGTTTTGAGAAAAATGGAGTCAAGTGAAAAGAATTCAGGCTGGGAAGACCTGATTTTCATTCCACCGGGAAAAAGCTACTCGCAGCACATTCAAGATGTGGTGAAACCCTGA

Protein Analysis

280

Amino Acids

30.99

Weight (kDa)

9.3

Isoelectric Point (pI)

56.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 13 - 275 5.4e-111 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33350 AT4G33350
fragaria_vesca FvH4_2g34490
malus_domestica MD08G1102400.v1.1 MD15G1083800.v1.1
prunus_persica Prupe.1G436300_v2.0.a1
pyrus_communis pycom08g08320 pycom15g07850 pycom15g07890
rosa_chinensis RchiOBHm_Chr6g0307851
rosa_laevigata RLG00000010693
rosa_multiflora Rmu_sc0003252.1_g000014
rosa_roxburghii Rroxscaffold_7G00160820
rosa_rugosa Rorug06G0361400
rosa_samantha Rh6AG470200 Rh6BG481200 Rh6CG486500 Rh6DG472700
rosa_wichuraiana Rw6G041050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 83, 178
AccII CGCG 2 cut(s) 25, 200
AciI CCGC 8 cut(s) 23, 76, 100, 118, 155, 231, 425, 540
AclWI GGATC 1 cut(s) 320
AcoI YGGCCR 3 cut(s) 228, 267, 276
AcsI RAATTY 1 cut(s) 760
AcuI CTGAAG 1 cut(s) 488
AcvI CACGTG 1 cut(s) 253
AcyI GRCGYC 2 cut(s) 84, 179
AfaI GTAC 1 cut(s) 461
AfiI CCNNNNNNNGG 2 cut(s) 201, 546
AgsI TTSAA 4 cut(s) 476, 512, 650, 821
AjnI CCWGG 3 cut(s) 264, 453, 688
AluBI AGCT 4 cut(s) 91, 243, 532, 804
AluI AGCT 4 cut(s) 91, 243, 532, 804
Alw21I GWGCWC 3 cut(s) 93, 318, 534
AlwI GGATC 1 cut(s) 320
AoxI GGCC 4 cut(s) 228, 267, 276, 336
ApeKI GCWGC 1 cut(s) 811
ApoI RAATTY 1 cut(s) 760
AspLEI GCGC 5 cut(s) 27, 86, 168, 174, 181
AspS9I GGNCC 1 cut(s) 686
AsuC2I CCSGG 2 cut(s) 271, 795
AsuHPI GGTGA 1 cut(s) 625
AsuII TTCGAA 1 cut(s) 299
AvaII GGWCC 1 cut(s) 686
BanI GGYRCC 2 cut(s) 83, 178
BanII GRGCYC 2 cut(s) 93, 534
BbrPI CACGTG 1 cut(s) 253
BbsI GAAGAC 2 cut(s) 114, 780
Bbv12I GWGCWC 3 cut(s) 93, 318, 534
BbvCI CCTCAGC 1 cut(s) 239
BbvI GCAGC 1 cut(s) 823
BccI CCATC 1 cut(s) 554
BceAI ACGGC 2 cut(s) 263, 291
BciT130I CCWGG 3 cut(s) 266, 455, 690
BciVI GTATCC 1 cut(s) 679
BcnI CCSGG 2 cut(s) 271, 795
BfoI RGCGCY 2 cut(s) 87, 182
BfuI GTATCC 1 cut(s) 679
BglI GCCNNNNNGGC 1 cut(s) 275
BisI GCNGC 5 cut(s) 119, 156, 231, 426, 812
BlsI GCNGC 5 cut(s) 120, 157, 232, 427, 813
Bme1390I CCNGG 5 cut(s) 266, 271, 455, 690, 795
Bme18I GGWCC 1 cut(s) 686
BmgT120I GGNCC 1 cut(s) 686
BmiI GGNNCC 3 cut(s) 85, 130, 180
BmrFI CCNGG 5 cut(s) 266, 271, 455, 690, 795
BmrI ACTGGG 1 cut(s) 500
BmsI GCATC 2 cut(s) 364, 686
BmuI ACTGGG 1 cut(s) 500
BpiI GAAGAC 2 cut(s) 114, 780
Bpu10I CCTNAGC 1 cut(s) 239
Bpu14I TTCGAA 1 cut(s) 299
BpuEI CTTGAG 2 cut(s) 378, 548
BpuMI CCSGG 2 cut(s) 271, 795
BsaAI YACGTR 1 cut(s) 253
BsaHI GRCGYC 2 cut(s) 84, 179
BsaJI CCNNGG 3 cut(s) 79, 636, 689
BsaXI ACNNNNNCTCC 2 cut(s) 555, 585
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 546
Bse118I RCCGGY 2 cut(s) 151, 181
Bse1I ACTGG 1 cut(s) 506
BseBI CCWGG 3 cut(s) 266, 455, 690
BseDI CCNNGG 3 cut(s) 79, 636, 689
BseGI GGATG 1 cut(s) 677
BseLI CCNNNNNNNGG 2 cut(s) 201, 546
BseMII CTCAG 2 cut(s) 224, 253
BseNI ACTGG 1 cut(s) 506
BseRI GAGGAG 2 cut(s) 180, 421
BseX3I CGGCCG 1 cut(s) 276
BseXI GCAGC 1 cut(s) 823
BseYI CCCAGC 1 cut(s) 768
Bsh1236I CGCG 2 cut(s) 25, 200
Bsh1285I CGRYCG 2 cut(s) 279, 404
BshFI GGCC 4 cut(s) 230, 269, 278, 338
BshNI GGYRCC 2 cut(s) 83, 178
BsiEI CGRYCG 2 cut(s) 279, 404
BsiHKAI GWGCWC 3 cut(s) 93, 318, 534
BsiSI CCGG 5 cut(s) 126, 152, 182, 270, 794
BslFI GGGAC 2 cut(s) 286, 422
BslI CCNNNNNNNGG 2 cut(s) 201, 546
BsmFI GGGAC 2 cut(s) 286, 422
BsmI GAATGC 3 cut(s) 383, 485, 526
BsnI GGCC 4 cut(s) 230, 269, 278, 338
Bsp119I TTCGAA 1 cut(s) 299
Bsp1286I GDGCHC 3 cut(s) 93, 318, 534
Bsp1407I TGTACA 1 cut(s) 459
Bsp143I GATC 2 cut(s) 325, 401
BspACI CCGC 8 cut(s) 23, 76, 100, 118, 155, 231, 425, 540
BspANI GGCC 4 cut(s) 230, 269, 278, 338
BspCNI CTCAG 2 cut(s) 223, 252
BspFNI CGCG 2 cut(s) 25, 200
BspLI GGNNCC 3 cut(s) 85, 130, 180
BspPI GGATC 1 cut(s) 320
BspQI GCTCTTC 1 cut(s) 254
BspT104I TTCGAA 1 cut(s) 299
BspT107I GGYRCC 2 cut(s) 83, 178
BsrFI RCCGGY 2 cut(s) 151, 181
BsrGI TGTACA 1 cut(s) 459
BsrI ACTGG 1 cut(s) 506
BssAI RCCGGY 2 cut(s) 151, 181
BssECI CCNNGG 3 cut(s) 79, 636, 689
BssMI GATC 2 cut(s) 325, 401
BssNI GRCGYC 2 cut(s) 84, 179
BssT1I CCWWGG 1 cut(s) 636
Bst2UI CCWGG 3 cut(s) 266, 455, 690
Bst4CI ACNGT 1 cut(s) 289
Bst6I CTCTTC 1 cut(s) 254
BstACI GRCGYC 2 cut(s) 84, 179
BstAUI TGTACA 1 cut(s) 459
BstBAI YACGTR 1 cut(s) 253
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 7 cut(s) 123, 139, 153, 160, 164, 170, 207
BstDEI CTNAG 2 cut(s) 210, 239
BstF5I GGATG 1 cut(s) 677
BstFNI CGCG 2 cut(s) 25, 200
BstH2I RGCGCY 2 cut(s) 87, 182
BstHHI GCGC 5 cut(s) 27, 86, 168, 174, 181
BstKTI GATC 2 cut(s) 328, 404
BstMBI GATC 2 cut(s) 325, 401
BstMCI CGRYCG 2 cut(s) 279, 404
BstMWI GCNNNNNNNGC 6 cut(s) 22, 178, 206, 230, 275, 389
BstNI CCWGG 3 cut(s) 266, 455, 690
BstNSI RCATGY 2 cut(s) 172, 466
BstSCI CCNGG 5 cut(s) 264, 269, 453, 688, 793
BstUI CGCG 2 cut(s) 25, 200
BstV1I GCAGC 1 cut(s) 823
BstV2I GAAGAC 2 cut(s) 114, 780
BstZI CGGCCG 1 cut(s) 276
BsuI GTATCC 1 cut(s) 679
BsuRI GGCC 4 cut(s) 230, 269, 278, 338
BtsCI GGATG 1 cut(s) 677
BtsI GCAGTG 1 cut(s) 67
BtsIMutI CAGTG 1 cut(s) 67
Cac8I GCNNGC 7 cut(s) 123, 139, 153, 160, 164, 170, 207
CfoI GCGC 5 cut(s) 27, 86, 168, 174, 181
Cfr10I RCCGGY 2 cut(s) 151, 181
Cfr13I GGNCC 1 cut(s) 686
CsiI ACCWGGT 1 cut(s) 453
Csp6I GTAC 1 cut(s) 460
CviAII CATG 2 cut(s) 169, 463
CviQI GTAC 1 cut(s) 460
DdeI CTNAG 2 cut(s) 210, 239
DinI GGCGCC 2 cut(s) 85, 180
DpnI GATC 2 cut(s) 327, 403
DpnII GATC 2 cut(s) 325, 401
EaeI YGGCCR 3 cut(s) 228, 267, 276
EagI CGGCCG 1 cut(s) 276
Eam1104I CTCTTC 1 cut(s) 254
EarI CTCTTC 1 cut(s) 254
EciI GGCGGA 1 cut(s) 65
Ecl136II GAGCTC 2 cut(s) 91, 532
EclXI CGGCCG 1 cut(s) 276
Eco130I CCWWGG 1 cut(s) 636
Eco24I GRGCYC 2 cut(s) 93, 534
Eco47I GGWCC 1 cut(s) 686
Eco52I CGGCCG 1 cut(s) 276
Eco53kI GAGCTC 2 cut(s) 91, 532
Eco57I CTGAAG 1 cut(s) 488
Eco72I CACGTG 1 cut(s) 253
EcoICRI GAGCTC 2 cut(s) 91, 532
EcoRI GAATTC 1 cut(s) 760
EcoRII CCWGG 3 cut(s) 264, 453, 688
EcoT14I CCWWGG 1 cut(s) 636
EcoT38I GRGCYC 2 cut(s) 93, 534
EgeI GGCGCC 2 cut(s) 85, 180
EheI GGCGCC 2 cut(s) 85, 180
ErhI CCWWGG 1 cut(s) 636
FaeI CATG 2 cut(s) 172, 466
FaqI GGGAC 2 cut(s) 286, 422
FatI CATG 2 cut(s) 168, 462
FauI CCCGC 2 cut(s) 30, 107
Fnu4HI GCNGC 5 cut(s) 119, 156, 231, 426, 812
FokI GGATG 1 cut(s) 664
FriOI GRGCYC 2 cut(s) 93, 534
Fsp4HI GCNGC 5 cut(s) 119, 156, 231, 426, 812
GlaI GCGC 5 cut(s) 26, 85, 167, 173, 180
GluI GCNGC 5 cut(s) 119, 156, 231, 426, 812
GsaI CCCAGC 1 cut(s) 772
HaeII RGCGCY 2 cut(s) 87, 182
HaeIII GGCC 4 cut(s) 230, 269, 278, 338
HapII CCGG 5 cut(s) 126, 152, 182, 270, 794
HhaI GCGC 5 cut(s) 27, 86, 168, 174, 181
Hin1I GRCGYC 2 cut(s) 84, 179
Hin1II CATG 2 cut(s) 172, 466
Hin6I GCGC 5 cut(s) 25, 84, 166, 172, 179
HinP1I GCGC 5 cut(s) 25, 84, 166, 172, 179
HinfI GANTC 2 cut(s) 5, 746
HpaII CCGG 5 cut(s) 126, 152, 182, 270, 794
HphI GGTGA 1 cut(s) 625
Hpy166II GTNNAC 1 cut(s) 460
Hpy188I TCNGA 4 cut(s) 213, 247, 325, 583
Hpy188III TCNNGA 5 cut(s) 439, 449, 672, 681, 821
Hpy8I GTNNAC 1 cut(s) 460
HpyAV CCTTC 5 cut(s) 64, 205, 463, 470, 596
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 3 cut(s) 485, 524, 554
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 178, 206, 230, 275, 389
HpyF3I CTNAG 2 cut(s) 210, 239
HpySE526I ACGT 1 cut(s) 252
Hsp92I GRCGYC 2 cut(s) 84, 179
Hsp92II CATG 2 cut(s) 172, 466
HspAI GCGC 5 cut(s) 25, 84, 166, 172, 179
KasI GGCGCC 2 cut(s) 83, 178
KroI GCCGGC 1 cut(s) 151
KroNI GCCGGC 1 cut(s) 153
Kzo9I GATC 2 cut(s) 325, 401
LguI GCTCTTC 1 cut(s) 254
Lsp1109I GCAGC 1 cut(s) 823
LweI GCATC 2 cut(s) 364, 686
MabI ACCWGGT 1 cut(s) 453
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 1 cut(s) 614
MalI GATC 2 cut(s) 327, 403
MboI GATC 2 cut(s) 325, 401
MboII GAAGA 8 cut(s) 119, 271, 383, 610, 613, 653, 737, 785
MhlI GDGCHC 3 cut(s) 93, 318, 534
MluCI AATT 5 cut(s) 295, 413, 480, 656, 760
Mly113I GGCGCC 2 cut(s) 84, 179
MlyI GAGTC 2 cut(s) 14, 755
MmeI TCCRAC 1 cut(s) 606
MreI CGCCGGCG 1 cut(s) 151
MroNI GCCGGC 1 cut(s) 151
MslI CAYNNNNRTG 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 542
MspI CCGG 5 cut(s) 126, 152, 182, 270, 794
MspR9I CCNGG 5 cut(s) 266, 271, 455, 690, 795
Mva1269I GAATGC 3 cut(s) 383, 485, 526
MvaI CCWGG 3 cut(s) 266, 455, 690
MvnI CGCG 2 cut(s) 25, 200
MwoI GCNNNNNNNGC 6 cut(s) 22, 178, 206, 230, 275, 389
NaeI GCCGGC 1 cut(s) 153
NarI GGCGCC 2 cut(s) 84, 179
NciI CCSGG 2 cut(s) 271, 795
NdeII GATC 2 cut(s) 325, 401
NgoMIV GCCGGC 1 cut(s) 151
NlaIII CATG 2 cut(s) 172, 466
NlaIV GGNNCC 3 cut(s) 85, 130, 180
NmeAIII GCCGAG 3 cut(s) 60, 112, 155
NspI RCATGY 2 cut(s) 172, 466
NspV TTCGAA 1 cut(s) 299
PaeI GCATGC 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 254
PcsI WCGNNNNNNNCGW 2 cut(s) 155, 375
PctI GAATGC 3 cut(s) 383, 485, 526
PdiI GCCGGC 1 cut(s) 153
PkrI GCNGC 5 cut(s) 120, 157, 232, 427, 813
Ple19I CGATCG 1 cut(s) 404
PleI GAGTC 2 cut(s) 13, 754
PluTI GGCGCC 2 cut(s) 87, 182
PmaCI CACGTG 1 cut(s) 253
PmlI CACGTG 1 cut(s) 253
PpsI GAGTC 2 cut(s) 13, 754
Ppu21I YACGTR 1 cut(s) 253
Psp124BI GAGCTC 2 cut(s) 93, 534
Psp6I CCWGG 3 cut(s) 264, 453, 688
PspCI CACGTG 1 cut(s) 253
PspFI CCCAGC 1 cut(s) 768
PspGI CCWGG 3 cut(s) 264, 453, 688
PspN4I GGNNCC 3 cut(s) 85, 130, 180
PspPI GGNCC 1 cut(s) 686
PvuI CGATCG 1 cut(s) 404
RsaI GTAC 1 cut(s) 461
RsaNI GTAC 1 cut(s) 460
RseI CAYNNNNRTG 1 cut(s) 372
SacI GAGCTC 2 cut(s) 93, 534
SapI GCTCTTC 1 cut(s) 254
SatI GCNGC 5 cut(s) 119, 156, 231, 426, 812
Sau3AI GATC 2 cut(s) 325, 401
Sau96I GGNCC 1 cut(s) 686
SchI GAGTC 2 cut(s) 14, 755
ScrFI CCNGG 5 cut(s) 266, 271, 455, 690, 795
SduI GDGCHC 3 cut(s) 93, 318, 534
SexAI ACCWGGT 1 cut(s) 453
SfaNI GCATC 2 cut(s) 364, 686
SfiI GGCCNNNNNGGCC 1 cut(s) 275
SfoI GGCGCC 2 cut(s) 85, 180
SfuI TTCGAA 1 cut(s) 299
SgrAI CRCCGGYG 1 cut(s) 151
SinI GGWCC 1 cut(s) 686
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 2 cut(s) 393, 527
SmoI CTYRAG 2 cut(s) 393, 527
SphI GCATGC 1 cut(s) 172
Sse9I AATT 5 cut(s) 295, 413, 480, 656, 760
SsiI CCGC 8 cut(s) 23, 76, 100, 118, 155, 231, 425, 540
SspDI GGCGCC 2 cut(s) 83, 178
SstI GAGCTC 2 cut(s) 93, 534
StyD4I CCNGG 5 cut(s) 264, 269, 453, 688, 793
StyI CCWWGG 1 cut(s) 636
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 1 cut(s) 255
TaqI TCGA 4 cut(s) 105, 299, 400, 404
TasI AATT 5 cut(s) 295, 413, 480, 656, 760
TatI WGTACW 1 cut(s) 459
TauI GCSGC 4 cut(s) 121, 158, 233, 428
TscAI CASTG 1 cut(s) 74
TseI GCWGC 1 cut(s) 811
TspDTI ATGAA 3 cut(s) 46, 611, 775
TspRI CASTG 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 686
XapI RAATTY 1 cut(s) 760
XceI RCATGY 2 cut(s) 172, 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.