Rh6DG472700

Protein TIC 22, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0012495
Physical Location & Seq
Forward (+)
63903335 .. 63907056
3722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG472700.1

Sequence Viewer

Length: 774 bp
ATGGAGTCCCCAAAACCCCCCTTGCGCCCTCTGCTCTCCCTCTCCACCTTCATACACCACCACTGCCTCCGCCTCGCCTCCGACCTCTCCAGCCGCCTCGAAGACACCAAACGCCTCGCCGGAAACTTCCTGCCGCCGGCGACCAGGCGCCTGCTTTCGGCCCCGGGCACTCCGCCGCTGGCTTGGGTGGGCCAGCCGAACCACGCCTTGGCCGCCACCACCGCGACTCTGAGCTCCGACCACGTGGCGAAGAGCCTAGTCGGCACGGCGGTGTATACGGTTAGCAATGCGAACAATGAGTTCGTGCTTATCTCTGACCCCAGTGAAGCCAAGTCCATTGGCCTCCTTTGCTTCCGCAAAGAAGACGCCGAAGCCTTCCTCGCTCAGGTTCGGACTAGGACACGGGAGTTAAGAAGTCCGGCGAAGGTCGTCCCAATCACTCTTGACCAGGTGTACATGCTGAAGGTAGAAGGAATTGCATTTAGGTTTTTGCCTGATCCAGTTCAAATAAAGAATGCACTTGAGCTCAAAGCTGCGGACAGGAGTGCATTTGATGGAGTTCCTGTTTTTCAGTCCGACCTTCTGGTTGTGAAGAAGAAAAACAAGCGTTACTGCCCAATATATTTCACTAAGGAAGATATAGAAAAGGAATTGTCAAAGGTTTCCAGATCATCCCGAGGTCCAGGGGTTTCTCAAAATATTATGGTATTAGATAAACTTCCATCAGTTTTGCTTCATTTATCATGCAAAGAAATAACTGTTTTAATGTTTTAG

Protein Analysis

257

Amino Acids

28.38

Weight (kDa)

9.47

Isoelectric Point (pI)

50.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 11 - 237 2.8e-92 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33350 AT4G33350
fragaria_vesca FvH4_2g34490
malus_domestica MD08G1102400.v1.1 MD15G1083800.v1.1
prunus_persica Prupe.1G436300_v2.0.a1
pyrus_communis pycom08g08320 pycom15g07850 pycom15g07890
rosa_chinensis RchiOBHm_Chr6g0307851
rosa_laevigata RLG00000010693
rosa_multiflora Rmu_sc0003252.1_g000014
rosa_roxburghii Rroxscaffold_7G00160820
rosa_rugosa Rorug06G0361400
rosa_samantha Rh6AG470200 Rh6BG481200 Rh6CG486500 Rh6DG472700
rosa_wichuraiana Rw6G041050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 147
AccB7I CCANNNNNTGG 1 cut(s) 208
AccI GTMKAC 1 cut(s) 275
AccII CGCG 1 cut(s) 224
AclWI GGATC 1 cut(s) 491
AcoI YGGCCR 1 cut(s) 210
AcuI CTGAAG 1 cut(s) 482
AcvI CACGTG 1 cut(s) 244
AcyI GRCGYC 2 cut(s) 148, 366
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 4 cut(s) 136, 157, 208, 385
AgsI TTSAA 1 cut(s) 506
AjnI CCWGG 3 cut(s) 143, 447, 682
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AleI CACNNNNGTG 1 cut(s) 269
AluBI AGCT 3 cut(s) 234, 526, 533
AluI AGCT 3 cut(s) 234, 526, 533
Alw21I GWGCWC 2 cut(s) 236, 528
AlwI GGATC 1 cut(s) 491
Ama87I CYCGRG 2 cut(s) 163, 675
AoxI GGCC 4 cut(s) 159, 190, 210, 340
ApeKI GCWGC 1 cut(s) 533
AspLEI GCGC 2 cut(s) 27, 150
AspS9I GGNCC 3 cut(s) 160, 190, 680
AsuC2I CCSGG 2 cut(s) 164, 165
AvaI CYCGRG 2 cut(s) 163, 675
AvaII GGWCC 1 cut(s) 680
BaeGI GKGCMC 1 cut(s) 170
BanI GGYRCC 1 cut(s) 147
BanII GRGCYC 2 cut(s) 236, 528
BbrPI CACGTG 1 cut(s) 244
BbsI GAAGAC 2 cut(s) 108, 369
Bbv12I GWGCWC 2 cut(s) 236, 528
BbvI GCAGC 1 cut(s) 520
BccI CCATC 2 cut(s) 548, 730
BceAI ACGGC 1 cut(s) 282
BciT130I CCWGG 3 cut(s) 145, 449, 684
BcnI CCSGG 2 cut(s) 164, 165
BfaI CTAG 2 cut(s) 257, 396
BfoI RGCGCY 1 cut(s) 151
BglI GCCNNNNNGGC 1 cut(s) 261
BisI GCNGC 5 cut(s) 94, 134, 176, 213, 534
BlsI GCNGC 5 cut(s) 95, 135, 177, 214, 535
Bme1390I CCNGG 5 cut(s) 145, 164, 165, 449, 684
Bme18I GGWCC 1 cut(s) 680
BmeT110I CYCGRG 2 cut(s) 163, 675
BmgT120I GGNCC 3 cut(s) 160, 190, 680
BmiI GGNNCC 2 cut(s) 149, 162
BmrFI CCNGG 5 cut(s) 145, 164, 165, 449, 684
BmrI ACTGGG 1 cut(s) 315
BmuI ACTGGG 1 cut(s) 315
BpiI GAAGAC 2 cut(s) 108, 369
BpmI CTGGAG 1 cut(s) 73
Bpu10I CCTNAGC 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 542
BpuMI CCSGG 2 cut(s) 164, 165
BsaAI YACGTR 1 cut(s) 244
BsaHI GRCGYC 2 cut(s) 148, 366
BsaJI CCNNGG 5 cut(s) 162, 163, 207, 676, 683
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 4 cut(s) 136, 157, 208, 385
Bse118I RCCGGY 1 cut(s) 136
Bse1I ACTGG 2 cut(s) 321, 500
Bse3DI GCAATG 1 cut(s) 292
BseBI CCWGG 3 cut(s) 145, 449, 684
BseDI CCNNGG 5 cut(s) 162, 163, 207, 676, 683
BseGI GGATG 1 cut(s) 671
BseLI CCNNNNNNNGG 4 cut(s) 136, 157, 208, 385
BseMI GCAATG 1 cut(s) 292
BseMII CTCAG 2 cut(s) 221, 398
BseNI ACTGG 2 cut(s) 321, 500
BseSI GKGCMC 1 cut(s) 170
BseXI GCAGC 1 cut(s) 520
Bsh1236I CGCG 1 cut(s) 224
BshFI GGCC 4 cut(s) 161, 192, 212, 342
BshNI GGYRCC 1 cut(s) 147
BsiHKAI GWGCWC 2 cut(s) 236, 528
BsiHKCI CYCGRG 2 cut(s) 163, 675
BsiSI CCGG 4 cut(s) 120, 137, 164, 419
BslFI GGGAC 1 cut(s) 416
BslI CCNNNNNNNGG 4 cut(s) 136, 157, 208, 385
BsmFI GGGAC 1 cut(s) 416
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 4 cut(s) 161, 192, 212, 342
BsoBI CYCGRG 2 cut(s) 163, 675
Bsp1286I GDGCHC 3 cut(s) 170, 236, 528
Bsp1407I TGTACA 1 cut(s) 453
Bsp143I GATC 2 cut(s) 496, 668
BspANI GGCC 4 cut(s) 161, 192, 212, 342
BspCNI CTCAG 2 cut(s) 222, 397
BspFNI CGCG 1 cut(s) 224
BspLI GGNNCC 2 cut(s) 149, 162
BspPI GGATC 1 cut(s) 491
BspQI GCTCTTC 1 cut(s) 245
BspT107I GGYRCC 1 cut(s) 147
BsrDI GCAATG 1 cut(s) 292
BsrFI RCCGGY 1 cut(s) 136
BsrGI TGTACA 1 cut(s) 453
BsrI ACTGG 2 cut(s) 321, 500
BssAI RCCGGY 1 cut(s) 136
BssECI CCNNGG 5 cut(s) 162, 163, 207, 676, 683
BssMI GATC 2 cut(s) 496, 668
BssNAI GTATAC 1 cut(s) 276
BssNI GRCGYC 2 cut(s) 148, 366
BssT1I CCWWGG 1 cut(s) 207
Bst1107I GTATAC 1 cut(s) 276
Bst2UI CCWGG 3 cut(s) 145, 449, 684
Bst4CI ACNGT 2 cut(s) 280, 760
Bst6I CTCTTC 1 cut(s) 245
BstACI GRCGYC 2 cut(s) 148, 366
BstAUI TGTACA 1 cut(s) 453
BstBAI YACGTR 1 cut(s) 244
BstC8I GCNNGC 4 cut(s) 138, 152, 180, 194
BstDEI CTNAG 3 cut(s) 230, 384, 630
BstENI CCTNNNNNAGG 1 cut(s) 383
BstF5I GGATG 1 cut(s) 671
BstFNI CGCG 1 cut(s) 224
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 2 cut(s) 27, 150
BstKTI GATC 2 cut(s) 499, 671
BstMBI GATC 2 cut(s) 496, 668
BstMWI GCNNNNNNNGC 6 cut(s) 31, 212, 221, 261, 348, 380
BstNI CCWGG 3 cut(s) 145, 449, 684
BstNSI RCATGY 1 cut(s) 460
BstSCI CCNGG 5 cut(s) 143, 162, 163, 447, 682
BstSLI GKGCMC 1 cut(s) 170
BstUI CGCG 1 cut(s) 224
BstV1I GCAGC 1 cut(s) 520
BstV2I GAAGAC 2 cut(s) 108, 369
BstZ17I GTATAC 1 cut(s) 276
BsuRI GGCC 4 cut(s) 161, 192, 212, 342
BtsCI GGATG 1 cut(s) 671
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 2 cut(s) 61, 328
Cac8I GCNNGC 4 cut(s) 138, 152, 180, 194
CfoI GCGC 2 cut(s) 27, 150
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 3 cut(s) 160, 190, 680
Cfr9I CCCGGG 1 cut(s) 163
CseI GACGC 1 cut(s) 374
CsiI ACCWGGT 1 cut(s) 447
Csp6I GTAC 1 cut(s) 454
CviAII CATG 2 cut(s) 457, 744
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 3 cut(s) 230, 384, 630
DinI GGCGCC 1 cut(s) 149
DpnI GATC 2 cut(s) 498, 670
DpnII GATC 2 cut(s) 496, 668
EaeI YGGCCR 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
EciI GGCGGA 2 cut(s) 59, 162
Ecl136II GAGCTC 2 cut(s) 234, 526
Eco130I CCWWGG 1 cut(s) 207
Eco24I GRGCYC 2 cut(s) 236, 528
Eco47I GGWCC 1 cut(s) 680
Eco53kI GAGCTC 2 cut(s) 234, 526
Eco57I CTGAAG 1 cut(s) 482
Eco72I CACGTG 1 cut(s) 244
Eco88I CYCGRG 2 cut(s) 163, 675
EcoICRI GAGCTC 2 cut(s) 234, 526
EcoNI CCTNNNNNAGG 1 cut(s) 383
EcoRII CCWGG 3 cut(s) 143, 447, 682
EcoT14I CCWWGG 1 cut(s) 207
EcoT38I GRGCYC 2 cut(s) 236, 528
EgeI GGCGCC 1 cut(s) 149
EheI GGCGCC 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 2 cut(s) 460, 747
FaiI YATR 7 cut(s) 53, 276, 458, 622, 641, 704, 745
FaqI GGGAC 1 cut(s) 416
FatI CATG 2 cut(s) 456, 743
FblI GTMKAC 1 cut(s) 275
Fnu4HI GCNGC 5 cut(s) 94, 134, 176, 213, 534
FokI GGATG 1 cut(s) 658
FriOI GRGCYC 2 cut(s) 236, 528
Fsp4HI GCNGC 5 cut(s) 94, 134, 176, 213, 534
FspBI CTAG 2 cut(s) 257, 396
GlaI GCGC 2 cut(s) 26, 149
GluI GCNGC 5 cut(s) 94, 134, 176, 213, 534
GsuI CTGGAG 1 cut(s) 73
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 4 cut(s) 161, 192, 212, 342
HapII CCGG 4 cut(s) 120, 137, 164, 419
HgaI GACGC 1 cut(s) 374
HhaI GCGC 2 cut(s) 27, 150
Hin1I GRCGYC 2 cut(s) 148, 366
Hin1II CATG 2 cut(s) 460, 747
Hin6I GCGC 2 cut(s) 25, 148
HinP1I GCGC 2 cut(s) 25, 148
HinfI GANTC 2 cut(s) 5, 226
HpaII CCGG 4 cut(s) 120, 137, 164, 419
Hpy166II GTNNAC 2 cut(s) 276, 454
Hpy188I TCNGA 6 cut(s) 82, 231, 238, 316, 393, 577
Hpy188III TCNNGA 3 cut(s) 443, 666, 675
Hpy8I GTNNAC 2 cut(s) 276, 454
HpyAV CCTTC 6 cut(s) 58, 385, 418, 457, 464, 590
HpyCH4III ACNGT 2 cut(s) 280, 760
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 4 cut(s) 479, 518, 548, 747
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 212, 221, 261, 348, 380
HpyF3I CTNAG 3 cut(s) 230, 384, 630
HpySE526I ACGT 1 cut(s) 243
Hsp92I GRCGYC 2 cut(s) 148, 366
Hsp92II CATG 2 cut(s) 460, 747
HspAI GCGC 2 cut(s) 25, 148
KasI GGCGCC 1 cut(s) 147
KroI GCCGGC 1 cut(s) 136
KroNI GCCGGC 1 cut(s) 138
Kzo9I GATC 2 cut(s) 496, 668
LguI GCTCTTC 1 cut(s) 245
LmnI GCTCC 1 cut(s) 239
Lsp1109I GCAGC 1 cut(s) 520
MabI ACCWGGT 1 cut(s) 447
MaeI CTAG 2 cut(s) 257, 396
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 1 cut(s) 608
MalI GATC 2 cut(s) 498, 670
MboI GATC 2 cut(s) 496, 668
MboII GAAGA 6 cut(s) 113, 262, 374, 604, 607, 647
MhlI GDGCHC 3 cut(s) 170, 236, 528
MluCI AATT 2 cut(s) 474, 650
Mly113I GGCGCC 1 cut(s) 148
MlyI GAGTC 2 cut(s) 14, 220
MmeI TCCRAC 3 cut(s) 105, 261, 600
MreI CGCCGGCG 1 cut(s) 136
MroNI GCCGGC 1 cut(s) 136
MseI TTAA 2 cut(s) 410, 764
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 178
MspI CCGG 4 cut(s) 120, 137, 164, 419
MspR9I CCNGG 5 cut(s) 145, 164, 165, 449, 684
Mva1269I GAATGC 1 cut(s) 520
MvaI CCWGG 3 cut(s) 145, 449, 684
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 6 cut(s) 31, 212, 221, 261, 348, 380
NaeI GCCGGC 1 cut(s) 138
NarI GGCGCC 1 cut(s) 148
NciI CCSGG 2 cut(s) 164, 165
NdeII GATC 2 cut(s) 496, 668
NgoMIV GCCGGC 1 cut(s) 136
NlaIII CATG 2 cut(s) 460, 747
NlaIV GGNNCC 2 cut(s) 149, 162
NspI RCATGY 1 cut(s) 460
OliI CACNNNNGTG 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 245
PctI GAATGC 1 cut(s) 520
PdiI GCCGGC 1 cut(s) 138
PflMI CCANNNNNTGG 1 cut(s) 208
PkrI GCNGC 5 cut(s) 95, 135, 177, 214, 535
PleI GAGTC 2 cut(s) 13, 220
PluTI GGCGCC 1 cut(s) 151
PmaCI CACGTG 1 cut(s) 244
PmlI CACGTG 1 cut(s) 244
PpsI GAGTC 2 cut(s) 13, 220
Ppu21I YACGTR 1 cut(s) 244
Psp124BI GAGCTC 2 cut(s) 236, 528
Psp6I CCWGG 3 cut(s) 143, 447, 682
PspCI CACGTG 1 cut(s) 244
PspGI CCWGG 3 cut(s) 143, 447, 682
PspN4I GGNNCC 2 cut(s) 149, 162
PspPI GGNCC 3 cut(s) 160, 190, 680
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
RseI CAYNNNNRTG 1 cut(s) 269
SacI GAGCTC 2 cut(s) 236, 528
SapI GCTCTTC 1 cut(s) 245
SaqAI TTAA 2 cut(s) 410, 764
SatI GCNGC 5 cut(s) 94, 134, 176, 213, 534
Sau3AI GATC 2 cut(s) 496, 668
Sau96I GGNCC 3 cut(s) 160, 190, 680
SchI GAGTC 2 cut(s) 14, 220
ScrFI CCNGG 5 cut(s) 145, 164, 165, 449, 684
SduI GDGCHC 3 cut(s) 170, 236, 528
SexAI ACCWGGT 1 cut(s) 447
SfoI GGCGCC 1 cut(s) 149
SgrAI CRCCGGYG 1 cut(s) 136
SinI GGWCC 1 cut(s) 680
SmaI CCCGGG 1 cut(s) 165
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 2 cut(s) 474, 650
SspDI GGCGCC 1 cut(s) 147
SspI AATATT 1 cut(s) 700
SspMI CTAG 2 cut(s) 257, 396
SstI GAGCTC 2 cut(s) 236, 528
StyD4I CCNGG 5 cut(s) 143, 162, 163, 447, 682
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 2 cut(s) 280, 760
TaiI ACGT 1 cut(s) 246
TaqI TCGA 1 cut(s) 99
TasI AATT 2 cut(s) 474, 650
TatI WGTACW 1 cut(s) 453
TauI GCSGC 4 cut(s) 96, 136, 178, 215
Tru1I TTAA 2 cut(s) 410, 764
Tru9I TTAA 2 cut(s) 410, 764
TscAI CASTG 2 cut(s) 68, 328
TseI GCWGC 1 cut(s) 533
TspDTI ATGAA 2 cut(s) 40, 725
TspMI CCCGGG 1 cut(s) 163
TspRI CASTG 2 cut(s) 68, 328
Van91I CCANNNNNTGG 1 cut(s) 208
VpaK11BI GGWCC 1 cut(s) 680
XagI CCTNNNNNAGG 1 cut(s) 383
XceI RCATGY 1 cut(s) 460
XmaI CCCGGG 1 cut(s) 163
XmiI GTMKAC 1 cut(s) 275
XspI CTAG 2 cut(s) 257, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.