Rh6AG470200

Protein TIC 22, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0012495
Physical Location & Seq
Forward (+)
65346163 .. 65348454
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG470200.1

Sequence Viewer

Length: 762 bp
ATGGAGTCCCCAAAGCCCTTGGGCCCTCTGCTCTCCCTCTCCACCTTCATACACCACCACTGCCTCCGCCTCGCCGCCGACCTCTCCAGCCGCCTCGAAGACACCAAACGCCTCGCCGGAAACTTCCTGCCGCCGGCGACCAGGCGCCTGCTTTCCGCCCCGGGCACTCCGCCTCTGGCTTGGGTGGGCCAACCGAACCATGCCTTGGCCGCCACCACCGCGACTCTGAGCTCCGACCACGTGGCGAAGAGCCTAGTCGGCACGGCGGTGTATACGGTTAGCAATGCGAACAATGAGTTCGTGCTTATCTCTGACCCCAGTGAAGCCAAGTCCATTGGCCTCCTTTGCTTCCGCAAAGAAGACGCCGAAGCCTTCCTCGCTCAGGTTCGGACTAGGACGCGAGAGTTGAGAAGTCCAGCGAAGGTCGTCCCAATCACTCTTGACCAGGTGTACATGCTGAAGGTAGAAGGAATTGCATTTAGGTTTTTGCCTGATCCAGTTCAAATAAAGAATGCACTTGAGCTCAAAGCTGCGGACAGGAGTGCATTTGATGGAGTTCCTGTTTTTCAGTCCGACCTTCTGGTTGTGAAGAAGAAAAACAAGCGTTACTGCCCAATATATTTCACTAAGGAAGATATAGAAAAGGAATTGTCAAAGGTTTCCAGATCATCCCGAGGACCAGGGGTTTCTCAAAATATTATGGTTGGTAGTCTGGAAGATGTTTTGAGAAAAATGGAGGTACTCATTCTGTTTACTGCATGA

Protein Analysis

253

Amino Acids

27.81

Weight (kDa)

9.36

Isoelectric Point (pI)

48.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 10 - 246 8.6e-99 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33350 AT4G33350
fragaria_vesca FvH4_2g34490
malus_domestica MD08G1102400.v1.1 MD15G1083800.v1.1
prunus_persica Prupe.1G436300_v2.0.a1
pyrus_communis pycom08g08320 pycom15g07850 pycom15g07890
rosa_chinensis RchiOBHm_Chr6g0307851
rosa_laevigata RLG00000010693
rosa_multiflora Rmu_sc0003252.1_g000014
rosa_roxburghii Rroxscaffold_7G00160820
rosa_rugosa Rorug06G0361400
rosa_samantha Rh6AG470200 Rh6BG481200 Rh6CG486500 Rh6DG472700
rosa_wichuraiana Rw6G041050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 144
AccB7I CCANNNNNTGG 1 cut(s) 205
AccI GTMKAC 1 cut(s) 272
AccII CGCG 2 cut(s) 221, 400
AclWI GGATC 1 cut(s) 488
AcoI YGGCCR 1 cut(s) 207
AcuI CTGAAG 1 cut(s) 479
AcvI CACGTG 1 cut(s) 241
AcyI GRCGYC 2 cut(s) 145, 363
AfaI GTAC 2 cut(s) 452, 741
AfiI CCNNNNNNNGG 3 cut(s) 133, 205, 382
AgsI TTSAA 1 cut(s) 503
AjnI CCWGG 3 cut(s) 140, 444, 679
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AleI CACNNNNGTG 1 cut(s) 266
AluBI AGCT 3 cut(s) 231, 523, 530
AluI AGCT 3 cut(s) 231, 523, 530
Alw21I GWGCWC 2 cut(s) 233, 525
AlwI GGATC 1 cut(s) 488
Ama87I CYCGRG 2 cut(s) 160, 672
AoxI GGCC 4 cut(s) 22, 187, 207, 337
ApaI GGGCCC 1 cut(s) 26
ApeKI GCWGC 1 cut(s) 530
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 4 cut(s) 22, 23, 187, 677
AsuC2I CCSGG 2 cut(s) 161, 162
AvaI CYCGRG 2 cut(s) 160, 672
AvaII GGWCC 1 cut(s) 677
BaeGI GKGCMC 2 cut(s) 26, 167
BanI GGYRCC 1 cut(s) 144
BanII GRGCYC 3 cut(s) 26, 233, 525
BbrPI CACGTG 1 cut(s) 241
BbsI GAAGAC 2 cut(s) 105, 366
Bbv12I GWGCWC 2 cut(s) 233, 525
BbvI GCAGC 1 cut(s) 517
BccI CCATC 1 cut(s) 545
BceAI ACGGC 1 cut(s) 279
BciT130I CCWGG 3 cut(s) 142, 446, 681
BcnI CCSGG 2 cut(s) 161, 162
BfaI CTAG 2 cut(s) 254, 393
BfoI RGCGCY 1 cut(s) 148
BglI GCCNNNNNGGC 1 cut(s) 258
BisI GCNGC 5 cut(s) 75, 91, 131, 210, 531
BlsI GCNGC 5 cut(s) 76, 92, 132, 211, 532
Bme1390I CCNGG 5 cut(s) 142, 161, 162, 446, 681
Bme18I GGWCC 1 cut(s) 677
BmeT110I CYCGRG 2 cut(s) 160, 672
BmgT120I GGNCC 4 cut(s) 22, 23, 187, 677
BmiI GGNNCC 2 cut(s) 24, 146
BmrFI CCNGG 5 cut(s) 142, 161, 162, 446, 681
BmrI ACTGGG 1 cut(s) 312
BmuI ACTGGG 1 cut(s) 312
BpiI GAAGAC 2 cut(s) 105, 366
BpmI CTGGAG 1 cut(s) 70
Bpu10I CCTNAGC 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 539
BpuMI CCSGG 2 cut(s) 161, 162
BsaAI YACGTR 1 cut(s) 241
BsaHI GRCGYC 2 cut(s) 145, 363
BsaJI CCNNGG 6 cut(s) 18, 159, 160, 204, 673, 680
BsaXI ACNNNNNCTCC 2 cut(s) 546, 576
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 205, 382
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 2 cut(s) 318, 497
Bse3DI GCAATG 1 cut(s) 289
BseBI CCWGG 3 cut(s) 142, 446, 681
BseDI CCNNGG 6 cut(s) 18, 159, 160, 204, 673, 680
BseGI GGATG 1 cut(s) 668
BseLI CCNNNNNNNGG 3 cut(s) 133, 205, 382
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 2 cut(s) 218, 395
BseNI ACTGG 2 cut(s) 318, 497
BseSI GKGCMC 2 cut(s) 26, 167
BseXI GCAGC 1 cut(s) 517
Bsh1236I CGCG 2 cut(s) 221, 400
BshFI GGCC 4 cut(s) 24, 189, 209, 339
BshNI GGYRCC 1 cut(s) 144
BsiHKAI GWGCWC 2 cut(s) 233, 525
BsiHKCI CYCGRG 2 cut(s) 160, 672
BsiSI CCGG 3 cut(s) 117, 134, 161
BslFI GGGAC 1 cut(s) 413
BslI CCNNNNNNNGG 3 cut(s) 133, 205, 382
BsmFI GGGAC 1 cut(s) 413
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 4 cut(s) 24, 189, 209, 339
BsoBI CYCGRG 2 cut(s) 160, 672
Bsp120I GGGCCC 1 cut(s) 22
Bsp1286I GDGCHC 4 cut(s) 26, 167, 233, 525
Bsp1407I TGTACA 1 cut(s) 450
Bsp143I GATC 2 cut(s) 493, 665
BspANI GGCC 4 cut(s) 24, 189, 209, 339
BspCNI CTCAG 2 cut(s) 219, 394
BspFNI CGCG 2 cut(s) 221, 400
BspLI GGNNCC 2 cut(s) 24, 146
BspPI GGATC 1 cut(s) 488
BspQI GCTCTTC 1 cut(s) 242
BspT107I GGYRCC 1 cut(s) 144
BsrDI GCAATG 1 cut(s) 289
BsrFI RCCGGY 1 cut(s) 133
BsrGI TGTACA 1 cut(s) 450
BsrI ACTGG 2 cut(s) 318, 497
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 6 cut(s) 18, 159, 160, 204, 673, 680
BssMI GATC 2 cut(s) 493, 665
BssNAI GTATAC 1 cut(s) 273
BssNI GRCGYC 2 cut(s) 145, 363
BssT1I CCWWGG 2 cut(s) 18, 204
Bst1107I GTATAC 1 cut(s) 273
Bst2UI CCWGG 3 cut(s) 142, 446, 681
Bst4CI ACNGT 1 cut(s) 277
Bst6I CTCTTC 1 cut(s) 242
BstACI GRCGYC 2 cut(s) 145, 363
BstAUI TGTACA 1 cut(s) 450
BstBAI YACGTR 1 cut(s) 241
BstC8I GCNNGC 2 cut(s) 135, 149
BstDEI CTNAG 3 cut(s) 227, 381, 627
BstENI CCTNNNNNAGG 1 cut(s) 380
BstF5I GGATG 1 cut(s) 668
BstFNI CGCG 2 cut(s) 221, 400
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 2 cut(s) 496, 668
BstMBI GATC 2 cut(s) 493, 665
BstMWI GCNNNNNNNGC 5 cut(s) 209, 218, 258, 345, 377
BstNI CCWGG 3 cut(s) 142, 446, 681
BstNSI RCATGY 1 cut(s) 457
BstSCI CCNGG 5 cut(s) 140, 159, 160, 444, 679
BstSLI GKGCMC 2 cut(s) 26, 167
BstUI CGCG 2 cut(s) 221, 400
BstV1I GCAGC 1 cut(s) 517
BstV2I GAAGAC 2 cut(s) 105, 366
BstZ17I GTATAC 1 cut(s) 273
BsuRI GGCC 4 cut(s) 24, 189, 209, 339
BtsCI GGATG 1 cut(s) 668
BtsI GCAGTG 1 cut(s) 58
BtsIMutI CAGTG 2 cut(s) 58, 325
Cac8I GCNNGC 2 cut(s) 135, 149
CfoI GCGC 1 cut(s) 147
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 4 cut(s) 22, 23, 187, 677
Cfr9I CCCGGG 1 cut(s) 160
CseI GACGC 2 cut(s) 371, 406
CsiI ACCWGGT 1 cut(s) 444
Csp6I GTAC 2 cut(s) 451, 740
CviAII CATG 3 cut(s) 200, 454, 759
CviQI GTAC 2 cut(s) 451, 740
DdeI CTNAG 3 cut(s) 227, 381, 627
DinI GGCGCC 1 cut(s) 146
DpnI GATC 2 cut(s) 495, 667
DpnII GATC 2 cut(s) 493, 665
EaeI YGGCCR 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 242
EarI CTCTTC 1 cut(s) 242
EciI GGCGGA 3 cut(s) 56, 145, 159
Ecl136II GAGCTC 2 cut(s) 231, 523
Eco130I CCWWGG 2 cut(s) 18, 204
Eco24I GRGCYC 3 cut(s) 26, 233, 525
Eco47I GGWCC 1 cut(s) 677
Eco53kI GAGCTC 2 cut(s) 231, 523
Eco57I CTGAAG 1 cut(s) 479
Eco72I CACGTG 1 cut(s) 241
Eco88I CYCGRG 2 cut(s) 160, 672
EcoICRI GAGCTC 2 cut(s) 231, 523
EcoNI CCTNNNNNAGG 1 cut(s) 380
EcoO109I RGGNCCY 1 cut(s) 23
EcoRII CCWGG 3 cut(s) 140, 444, 679
EcoT14I CCWWGG 2 cut(s) 18, 204
EcoT38I GRGCYC 3 cut(s) 26, 233, 525
EgeI GGCGCC 1 cut(s) 146
EheI GGCGCC 1 cut(s) 146
ErhI CCWWGG 2 cut(s) 18, 204
FaeI CATG 3 cut(s) 203, 457, 762
FaiI YATR 8 cut(s) 50, 201, 273, 455, 619, 638, 701, 760
FaqI GGGAC 1 cut(s) 413
FatI CATG 3 cut(s) 199, 453, 758
FblI GTMKAC 1 cut(s) 272
Fnu4HI GCNGC 5 cut(s) 75, 91, 131, 210, 531
FokI GGATG 1 cut(s) 655
FriOI GRGCYC 3 cut(s) 26, 233, 525
Fsp4HI GCNGC 5 cut(s) 75, 91, 131, 210, 531
FspBI CTAG 2 cut(s) 254, 393
GlaI GCGC 1 cut(s) 146
GluI GCNGC 5 cut(s) 75, 91, 131, 210, 531
GsuI CTGGAG 1 cut(s) 70
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 4 cut(s) 24, 189, 209, 339
HapII CCGG 3 cut(s) 117, 134, 161
HgaI GACGC 2 cut(s) 371, 406
HhaI GCGC 1 cut(s) 147
Hin1I GRCGYC 2 cut(s) 145, 363
Hin1II CATG 3 cut(s) 203, 457, 762
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HinfI GANTC 2 cut(s) 5, 223
HpaII CCGG 3 cut(s) 117, 134, 161
Hpy166II GTNNAC 3 cut(s) 273, 451, 753
Hpy188I TCNGA 5 cut(s) 228, 235, 313, 390, 574
Hpy188III TCNNGA 4 cut(s) 440, 663, 672, 713
Hpy8I GTNNAC 3 cut(s) 273, 451, 753
HpyAV CCTTC 6 cut(s) 55, 382, 415, 454, 461, 587
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 4 cut(s) 476, 515, 545, 758
HpyF10VI GCNNNNNNNGC 5 cut(s) 209, 218, 258, 345, 377
HpyF3I CTNAG 3 cut(s) 227, 381, 627
HpySE526I ACGT 1 cut(s) 240
Hsp92I GRCGYC 2 cut(s) 145, 363
Hsp92II CATG 3 cut(s) 203, 457, 762
HspAI GCGC 1 cut(s) 145
KasI GGCGCC 1 cut(s) 144
KroI GCCGGC 1 cut(s) 133
KroNI GCCGGC 1 cut(s) 135
Kzo9I GATC 2 cut(s) 493, 665
LguI GCTCTTC 1 cut(s) 242
LmnI GCTCC 1 cut(s) 236
Lsp1109I GCAGC 1 cut(s) 517
MabI ACCWGGT 1 cut(s) 444
MaeI CTAG 2 cut(s) 254, 393
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 605
MalI GATC 2 cut(s) 495, 667
MboI GATC 2 cut(s) 493, 665
MboII GAAGA 7 cut(s) 110, 259, 371, 601, 604, 644, 728
MhlI GDGCHC 4 cut(s) 26, 167, 233, 525
MluCI AATT 2 cut(s) 471, 647
Mly113I GGCGCC 1 cut(s) 145
MlyI GAGTC 2 cut(s) 14, 217
MmeI TCCRAC 2 cut(s) 258, 597
MreI CGCCGGCG 1 cut(s) 133
MroNI GCCGGC 1 cut(s) 133
MslI CAYNNNNRTG 1 cut(s) 266
MspI CCGG 3 cut(s) 117, 134, 161
MspR9I CCNGG 5 cut(s) 142, 161, 162, 446, 681
Mva1269I GAATGC 1 cut(s) 517
MvaI CCWGG 3 cut(s) 142, 446, 681
MvnI CGCG 2 cut(s) 221, 400
MwoI GCNNNNNNNGC 5 cut(s) 209, 218, 258, 345, 377
NaeI GCCGGC 1 cut(s) 135
NarI GGCGCC 1 cut(s) 145
NciI CCSGG 2 cut(s) 161, 162
NdeII GATC 2 cut(s) 493, 665
NgoMIV GCCGGC 1 cut(s) 133
NlaIII CATG 3 cut(s) 203, 457, 762
NlaIV GGNNCC 2 cut(s) 24, 146
NspI RCATGY 1 cut(s) 457
OliI CACNNNNGTG 1 cut(s) 266
PciSI GCTCTTC 1 cut(s) 242
PctI GAATGC 1 cut(s) 517
PdiI GCCGGC 1 cut(s) 135
PflMI CCANNNNNTGG 1 cut(s) 205
PkrI GCNGC 5 cut(s) 76, 92, 132, 211, 532
PleI GAGTC 2 cut(s) 13, 217
PluTI GGCGCC 1 cut(s) 148
PmaCI CACGTG 1 cut(s) 241
PmlI CACGTG 1 cut(s) 241
PpsI GAGTC 2 cut(s) 13, 217
Ppu21I YACGTR 1 cut(s) 241
Psp124BI GAGCTC 2 cut(s) 233, 525
Psp6I CCWGG 3 cut(s) 140, 444, 679
PspCI CACGTG 1 cut(s) 241
PspGI CCWGG 3 cut(s) 140, 444, 679
PspN4I GGNNCC 2 cut(s) 24, 146
PspOMI GGGCCC 1 cut(s) 22
PspPI GGNCC 4 cut(s) 22, 23, 187, 677
RsaI GTAC 2 cut(s) 452, 741
RsaNI GTAC 2 cut(s) 451, 740
RseI CAYNNNNRTG 1 cut(s) 266
SacI GAGCTC 2 cut(s) 233, 525
SapI GCTCTTC 1 cut(s) 242
SatI GCNGC 5 cut(s) 75, 91, 131, 210, 531
Sau3AI GATC 2 cut(s) 493, 665
Sau96I GGNCC 4 cut(s) 22, 23, 187, 677
SchI GAGTC 2 cut(s) 14, 217
ScrFI CCNGG 5 cut(s) 142, 161, 162, 446, 681
SduI GDGCHC 4 cut(s) 26, 167, 233, 525
SexAI ACCWGGT 1 cut(s) 444
SfoI GGCGCC 1 cut(s) 146
SgrAI CRCCGGYG 1 cut(s) 133
SinI GGWCC 1 cut(s) 677
SmaI CCCGGG 1 cut(s) 162
SmiMI CAYNNNNRTG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
Sse9I AATT 2 cut(s) 471, 647
SspDI GGCGCC 1 cut(s) 144
SspI AATATT 1 cut(s) 697
SspMI CTAG 2 cut(s) 254, 393
SstI GAGCTC 2 cut(s) 233, 525
StyD4I CCNGG 5 cut(s) 140, 159, 160, 444, 679
StyI CCWWGG 2 cut(s) 18, 204
TaaI ACNGT 1 cut(s) 277
TaiI ACGT 1 cut(s) 243
TaqI TCGA 1 cut(s) 96
TasI AATT 2 cut(s) 471, 647
TatI WGTACW 1 cut(s) 450
TauI GCSGC 4 cut(s) 77, 93, 133, 212
TscAI CASTG 2 cut(s) 65, 325
TseI GCWGC 1 cut(s) 530
TspDTI ATGAA 1 cut(s) 37
TspMI CCCGGG 1 cut(s) 160
TspRI CASTG 2 cut(s) 65, 325
Van91I CCANNNNNTGG 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 677
XagI CCTNNNNNAGG 1 cut(s) 380
XceI RCATGY 1 cut(s) 457
XmaI CCCGGG 1 cut(s) 160
XmiI GTMKAC 1 cut(s) 272
XspI CTAG 2 cut(s) 254, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.