Rw6G041050

Protein TIC 22, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012495
Physical Location & Seq
Forward (+)
64808182 .. 64811320
3139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G041050.1

Sequence Viewer

Length: 834 bp
ATGGAGTCCCCAAAGCCCTTGGGCCCTCTGCTCTCTCTCTCCACCTTCATACACCACCACTGCCTCCGCCTCGCCGCCGACCTCTCCAGCCGCCTCGAAGACACCAAACGCCTCGCCGGAAACTTCCTGCCGCCGGCGACCAGGCGCCTGCTTTCCGCCCCGGGCACTCCGCCTCTGGCTTGGGTGGGCCAACCGAACCATGCCTTGGCCGCCGCCACCACCGCGACTCTGAGCTCCGACCACGTGGCGAAGAGCCTAGTCGGCACGGCGGTGTATACGGTTAGCAATGCGAACAATGAGTTCGTGCTTATCTCTGACCCCAGTGAAGCCAAGTCCATTGGCCTCCTTTGCTTCCGCAAAGAAGACGCCGAAGCCTTCCTCGCTCAGGTTCGGACTAGGACACGAGAGTTGAGAAGTCCAGCGAAGGTCGTCCCAATCACTCTTGACCAGGTGTACATGCTGAAGGTAGAAGGAATTGCATTTAGGTTTTTGCCTGATCCCGTTCAAATAAAGAATGCACTTGAGCTCAAAGCTGCGGACAGGAGTGCATTTGATGGAGTTCCTGTTTTTCAGTCCGACCTTCTGGTTGTGAAGAAGAAAAACAAGCGTTACTGCCCAATATATTTCACTAAGGAAGATATAGAAAAGGAATTGTCAAAGGTTTCCAGATCATCCCGAGGACCAGGGGTTTCTCAAAATATTATGGTTGGTAGTCTGGAAGATGTTTTGAGAAAAATGGAGTCAAGTGAGAAGAATTCGGGCTGGGAAGATCTGATTTTTATTCCACCTGGCAAAAGCTACTCCCAACACATTCAAGATGTGGTTAAAAAATGA

Protein Analysis

277

Amino Acids

30.52

Weight (kDa)

9.26

Isoelectric Point (pI)

51.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 10 - 273 1.2e-113 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33350 AT4G33350
fragaria_vesca FvH4_2g34490
malus_domestica MD08G1102400.v1.1 MD15G1083800.v1.1
prunus_persica Prupe.1G436300_v2.0.a1
pyrus_communis pycom08g08320 pycom15g07850 pycom15g07890
rosa_chinensis RchiOBHm_Chr6g0307851
rosa_laevigata RLG00000010693
rosa_multiflora Rmu_sc0003252.1_g000014
rosa_roxburghii Rroxscaffold_7G00160820
rosa_rugosa Rorug06G0361400
rosa_samantha Rh6AG470200 Rh6BG481200 Rh6CG486500 Rh6DG472700
rosa_wichuraiana Rw6G041050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 144
AccB7I CCANNNNNTGG 1 cut(s) 205
AccI GTMKAC 1 cut(s) 275
AccII CGCG 1 cut(s) 224
AclWI GGATC 1 cut(s) 491
AcoI YGGCCR 1 cut(s) 207
AcsI RAATTY 1 cut(s) 754
AcuI CTGAAG 1 cut(s) 482
AcvI CACGTG 1 cut(s) 244
AcyI GRCGYC 2 cut(s) 145, 366
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 3 cut(s) 133, 205, 385
AgsI TTSAA 2 cut(s) 506, 815
AjnI CCWGG 4 cut(s) 140, 447, 682, 787
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AleI CACNNNNGTG 1 cut(s) 269
AluBI AGCT 4 cut(s) 234, 526, 533, 798
AluI AGCT 4 cut(s) 234, 526, 533, 798
Alw21I GWGCWC 2 cut(s) 236, 528
AlwI GGATC 1 cut(s) 491
Ama87I CYCGRG 2 cut(s) 160, 675
AoxI GGCC 4 cut(s) 22, 187, 207, 340
ApaI GGGCCC 1 cut(s) 26
ApeKI GCWGC 1 cut(s) 533
ApoI RAATTY 1 cut(s) 754
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 4 cut(s) 22, 23, 187, 680
AsuC2I CCSGG 2 cut(s) 161, 162
AvaI CYCGRG 2 cut(s) 160, 675
AvaII GGWCC 1 cut(s) 680
BaeGI GKGCMC 2 cut(s) 26, 167
BanI GGYRCC 1 cut(s) 144
BanII GRGCYC 3 cut(s) 26, 236, 528
BauI CACGAG 1 cut(s) 402
BbrPI CACGTG 1 cut(s) 244
BbsI GAAGAC 2 cut(s) 105, 369
Bbv12I GWGCWC 2 cut(s) 236, 528
BbvI GCAGC 1 cut(s) 520
BccI CCATC 1 cut(s) 548
BceAI ACGGC 1 cut(s) 282
BciT130I CCWGG 4 cut(s) 142, 449, 684, 789
BcnI CCSGG 2 cut(s) 161, 162
BfaI CTAG 2 cut(s) 257, 396
BfoI RGCGCY 1 cut(s) 148
BglI GCCNNNNNGGC 1 cut(s) 261
BglII AGATCT 1 cut(s) 769
BisI GCNGC 6 cut(s) 75, 91, 131, 210, 213, 534
BlsI GCNGC 6 cut(s) 76, 92, 132, 211, 214, 535
Bme1390I CCNGG 6 cut(s) 142, 161, 162, 449, 684, 789
Bme18I GGWCC 1 cut(s) 680
BmeT110I CYCGRG 2 cut(s) 160, 675
BmgT120I GGNCC 4 cut(s) 22, 23, 187, 680
BmiI GGNNCC 2 cut(s) 24, 146
BmrFI CCNGG 6 cut(s) 142, 161, 162, 449, 684, 789
BmrI ACTGGG 1 cut(s) 315
BmuI ACTGGG 1 cut(s) 315
BpiI GAAGAC 2 cut(s) 105, 369
BpmI CTGGAG 1 cut(s) 70
Bpu10I CCTNAGC 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 542
BpuMI CCSGG 2 cut(s) 161, 162
BsaAI YACGTR 1 cut(s) 244
BsaHI GRCGYC 2 cut(s) 145, 366
BsaJI CCNNGG 6 cut(s) 18, 159, 160, 204, 676, 683
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 205, 385
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 292
BseBI CCWGG 4 cut(s) 142, 449, 684, 789
BseDI CCNNGG 6 cut(s) 18, 159, 160, 204, 676, 683
BseGI GGATG 1 cut(s) 671
BseLI CCNNNNNNNGG 3 cut(s) 133, 205, 385
BseMI GCAATG 1 cut(s) 292
BseMII CTCAG 2 cut(s) 221, 398
BseNI ACTGG 1 cut(s) 321
BseSI GKGCMC 2 cut(s) 26, 167
BseXI GCAGC 1 cut(s) 520
BseYI CCCAGC 1 cut(s) 762
Bsh1236I CGCG 1 cut(s) 224
BshFI GGCC 4 cut(s) 24, 189, 209, 342
BshNI GGYRCC 1 cut(s) 144
BsiHKAI GWGCWC 2 cut(s) 236, 528
BsiHKCI CYCGRG 2 cut(s) 160, 675
BsiSI CCGG 3 cut(s) 117, 134, 161
BslFI GGGAC 1 cut(s) 416
BslI CCNNNNNNNGG 3 cut(s) 133, 205, 385
BsmFI GGGAC 1 cut(s) 416
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 4 cut(s) 24, 189, 209, 342
BsoBI CYCGRG 2 cut(s) 160, 675
Bsp120I GGGCCC 1 cut(s) 22
Bsp1286I GDGCHC 4 cut(s) 26, 167, 236, 528
Bsp1407I TGTACA 1 cut(s) 453
Bsp143I GATC 3 cut(s) 496, 668, 769
BspANI GGCC 4 cut(s) 24, 189, 209, 342
BspCNI CTCAG 2 cut(s) 222, 397
BspFNI CGCG 1 cut(s) 224
BspLI GGNNCC 2 cut(s) 24, 146
BspPI GGATC 1 cut(s) 491
BspQI GCTCTTC 1 cut(s) 245
BspT107I GGYRCC 1 cut(s) 144
BsrDI GCAATG 1 cut(s) 292
BsrFI RCCGGY 1 cut(s) 133
BsrGI TGTACA 1 cut(s) 453
BsrI ACTGG 1 cut(s) 321
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 6 cut(s) 18, 159, 160, 204, 676, 683
BssMI GATC 3 cut(s) 496, 668, 769
BssNAI GTATAC 1 cut(s) 276
BssNI GRCGYC 2 cut(s) 145, 366
BssSI CACGAG 1 cut(s) 402
BssT1I CCWWGG 2 cut(s) 18, 204
Bst1107I GTATAC 1 cut(s) 276
Bst2BI CACGAG 1 cut(s) 402
Bst2UI CCWGG 4 cut(s) 142, 449, 684, 789
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 1 cut(s) 245
BstACI GRCGYC 2 cut(s) 145, 366
BstAUI TGTACA 1 cut(s) 453
BstBAI YACGTR 1 cut(s) 244
BstC8I GCNNGC 2 cut(s) 135, 149
BstDEI CTNAG 3 cut(s) 230, 384, 630
BstENI CCTNNNNNAGG 1 cut(s) 383
BstF5I GGATG 1 cut(s) 671
BstFNI CGCG 1 cut(s) 224
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 3 cut(s) 499, 671, 772
BstMBI GATC 3 cut(s) 496, 668, 769
BstMWI GCNNNNNNNGC 5 cut(s) 209, 221, 261, 348, 380
BstNI CCWGG 4 cut(s) 142, 449, 684, 789
BstNSI RCATGY 1 cut(s) 460
BstSCI CCNGG 6 cut(s) 140, 159, 160, 447, 682, 787
BstSLI GKGCMC 2 cut(s) 26, 167
BstUI CGCG 1 cut(s) 224
BstV1I GCAGC 1 cut(s) 520
BstV2I GAAGAC 2 cut(s) 105, 369
BstX2I RGATCY 1 cut(s) 769
BstYI RGATCY 1 cut(s) 769
BstZ17I GTATAC 1 cut(s) 276
BsuRI GGCC 4 cut(s) 24, 189, 209, 342
BtsCI GGATG 1 cut(s) 671
BtsI GCAGTG 1 cut(s) 58
BtsIMutI CAGTG 2 cut(s) 58, 328
Cac8I GCNNGC 2 cut(s) 135, 149
CfoI GCGC 1 cut(s) 147
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 4 cut(s) 22, 23, 187, 680
Cfr9I CCCGGG 1 cut(s) 160
CseI GACGC 1 cut(s) 374
CsiI ACCWGGT 1 cut(s) 447
Csp6I GTAC 1 cut(s) 454
CviAII CATG 2 cut(s) 200, 457
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 3 cut(s) 230, 384, 630
DinI GGCGCC 1 cut(s) 146
DpnI GATC 3 cut(s) 498, 670, 771
DpnII GATC 3 cut(s) 496, 668, 769
EaeI YGGCCR 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
EciI GGCGGA 3 cut(s) 56, 145, 159
Ecl136II GAGCTC 2 cut(s) 234, 526
Eco130I CCWWGG 2 cut(s) 18, 204
Eco24I GRGCYC 3 cut(s) 26, 236, 528
Eco47I GGWCC 1 cut(s) 680
Eco53kI GAGCTC 2 cut(s) 234, 526
Eco57I CTGAAG 1 cut(s) 482
Eco72I CACGTG 1 cut(s) 244
Eco88I CYCGRG 2 cut(s) 160, 675
EcoICRI GAGCTC 2 cut(s) 234, 526
EcoNI CCTNNNNNAGG 1 cut(s) 383
EcoO109I RGGNCCY 1 cut(s) 23
EcoRI GAATTC 1 cut(s) 754
EcoRII CCWGG 4 cut(s) 140, 447, 682, 787
EcoT14I CCWWGG 2 cut(s) 18, 204
EcoT38I GRGCYC 3 cut(s) 26, 236, 528
EgeI GGCGCC 1 cut(s) 146
EheI GGCGCC 1 cut(s) 146
ErhI CCWWGG 2 cut(s) 18, 204
FaeI CATG 2 cut(s) 203, 460
FaiI YATR 7 cut(s) 50, 201, 276, 458, 622, 641, 704
FaqI GGGAC 1 cut(s) 416
FatI CATG 2 cut(s) 199, 456
FblI GTMKAC 1 cut(s) 275
Fnu4HI GCNGC 6 cut(s) 75, 91, 131, 210, 213, 534
FokI GGATG 1 cut(s) 658
FriOI GRGCYC 3 cut(s) 26, 236, 528
Fsp4HI GCNGC 6 cut(s) 75, 91, 131, 210, 213, 534
FspBI CTAG 2 cut(s) 257, 396
GlaI GCGC 1 cut(s) 146
GluI GCNGC 6 cut(s) 75, 91, 131, 210, 213, 534
GsaI CCCAGC 1 cut(s) 766
GsuI CTGGAG 1 cut(s) 70
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 4 cut(s) 24, 189, 209, 342
HapII CCGG 3 cut(s) 117, 134, 161
HgaI GACGC 1 cut(s) 374
HhaI GCGC 1 cut(s) 147
Hin1I GRCGYC 2 cut(s) 145, 366
Hin1II CATG 2 cut(s) 203, 460
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HinfI GANTC 3 cut(s) 5, 226, 740
HpaII CCGG 3 cut(s) 117, 134, 161
Hpy166II GTNNAC 2 cut(s) 276, 454
Hpy188I TCNGA 6 cut(s) 231, 238, 316, 393, 577, 774
Hpy188III TCNNGA 5 cut(s) 443, 666, 675, 716, 815
Hpy8I GTNNAC 2 cut(s) 276, 454
HpyAV CCTTC 6 cut(s) 55, 385, 418, 457, 464, 590
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 3 cut(s) 479, 518, 548
HpyF10VI GCNNNNNNNGC 5 cut(s) 209, 221, 261, 348, 380
HpyF3I CTNAG 3 cut(s) 230, 384, 630
HpySE526I ACGT 1 cut(s) 243
Hsp92I GRCGYC 2 cut(s) 145, 366
Hsp92II CATG 2 cut(s) 203, 460
HspAI GCGC 1 cut(s) 145
KasI GGCGCC 1 cut(s) 144
KroI GCCGGC 1 cut(s) 133
KroNI GCCGGC 1 cut(s) 135
Kzo9I GATC 3 cut(s) 496, 668, 769
LguI GCTCTTC 1 cut(s) 245
LmnI GCTCC 1 cut(s) 239
Lsp1109I GCAGC 1 cut(s) 520
MabI ACCWGGT 1 cut(s) 447
MaeI CTAG 2 cut(s) 257, 396
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 1 cut(s) 608
MalI GATC 3 cut(s) 498, 670, 771
MboI GATC 3 cut(s) 496, 668, 769
MboII GAAGA 9 cut(s) 110, 262, 374, 604, 607, 647, 731, 763, 779
MflI RGATCY 1 cut(s) 769
MhlI GDGCHC 4 cut(s) 26, 167, 236, 528
MluCI AATT 3 cut(s) 474, 650, 754
Mly113I GGCGCC 1 cut(s) 145
MlyI GAGTC 3 cut(s) 14, 220, 749
MmeI TCCRAC 2 cut(s) 261, 600
MreI CGCCGGCG 1 cut(s) 133
MroNI GCCGGC 1 cut(s) 133
MseI TTAA 1 cut(s) 825
MslI CAYNNNNRTG 1 cut(s) 269
MspI CCGG 3 cut(s) 117, 134, 161
MspR9I CCNGG 6 cut(s) 142, 161, 162, 449, 684, 789
Mva1269I GAATGC 1 cut(s) 520
MvaI CCWGG 4 cut(s) 142, 449, 684, 789
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 5 cut(s) 209, 221, 261, 348, 380
NaeI GCCGGC 1 cut(s) 135
NarI GGCGCC 1 cut(s) 145
NciI CCSGG 2 cut(s) 161, 162
NdeII GATC 3 cut(s) 496, 668, 769
NgoMIV GCCGGC 1 cut(s) 133
NlaIII CATG 2 cut(s) 203, 460
NlaIV GGNNCC 2 cut(s) 24, 146
NspI RCATGY 1 cut(s) 460
OliI CACNNNNGTG 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 245
PctI GAATGC 1 cut(s) 520
PdiI GCCGGC 1 cut(s) 135
PflMI CCANNNNNTGG 1 cut(s) 205
PkrI GCNGC 6 cut(s) 76, 92, 132, 211, 214, 535
PleI GAGTC 3 cut(s) 13, 220, 748
PluTI GGCGCC 1 cut(s) 148
PmaCI CACGTG 1 cut(s) 244
PmlI CACGTG 1 cut(s) 244
PpsI GAGTC 3 cut(s) 13, 220, 748
Ppu21I YACGTR 1 cut(s) 244
Psp124BI GAGCTC 2 cut(s) 236, 528
Psp6I CCWGG 4 cut(s) 140, 447, 682, 787
PspCI CACGTG 1 cut(s) 244
PspFI CCCAGC 1 cut(s) 762
PspGI CCWGG 4 cut(s) 140, 447, 682, 787
PspN4I GGNNCC 2 cut(s) 24, 146
PspOMI GGGCCC 1 cut(s) 22
PspPI GGNCC 4 cut(s) 22, 23, 187, 680
PsuI RGATCY 1 cut(s) 769
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
RseI CAYNNNNRTG 1 cut(s) 269
SacI GAGCTC 2 cut(s) 236, 528
SapI GCTCTTC 1 cut(s) 245
SaqAI TTAA 1 cut(s) 825
SatI GCNGC 6 cut(s) 75, 91, 131, 210, 213, 534
Sau3AI GATC 3 cut(s) 496, 668, 769
Sau96I GGNCC 4 cut(s) 22, 23, 187, 680
SchI GAGTC 3 cut(s) 14, 220, 749
ScrFI CCNGG 6 cut(s) 142, 161, 162, 449, 684, 789
SduI GDGCHC 4 cut(s) 26, 167, 236, 528
SexAI ACCWGGT 1 cut(s) 447
SfoI GGCGCC 1 cut(s) 146
SgrAI CRCCGGYG 1 cut(s) 133
SinI GGWCC 1 cut(s) 680
SmaI CCCGGG 1 cut(s) 162
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 3 cut(s) 474, 650, 754
SspDI GGCGCC 1 cut(s) 144
SspI AATATT 1 cut(s) 700
SspMI CTAG 2 cut(s) 257, 396
SstI GAGCTC 2 cut(s) 236, 528
StyD4I CCNGG 6 cut(s) 140, 159, 160, 447, 682, 787
StyI CCWWGG 2 cut(s) 18, 204
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 1 cut(s) 246
TaqI TCGA 1 cut(s) 96
TasI AATT 3 cut(s) 474, 650, 754
TatI WGTACW 1 cut(s) 453
TauI GCSGC 5 cut(s) 77, 93, 133, 212, 215
Tru1I TTAA 1 cut(s) 825
Tru9I TTAA 1 cut(s) 825
TscAI CASTG 2 cut(s) 65, 328
TseI GCWGC 1 cut(s) 533
TspDTI ATGAA 1 cut(s) 37
TspMI CCCGGG 1 cut(s) 160
TspRI CASTG 2 cut(s) 65, 328
Van91I CCANNNNNTGG 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 680
XagI CCTNNNNNAGG 1 cut(s) 383
XapI RAATTY 1 cut(s) 754
XceI RCATGY 1 cut(s) 460
XmaI CCCGGG 1 cut(s) 160
XmiI GTMKAC 1 cut(s) 275
XspI CTAG 2 cut(s) 257, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.