FvH4_2g34490

Protein TIC 22, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012495
Physical Location & Seq
Reverse (-)
25613829 .. 25616199
2371 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34490.t1

Sequence Viewer

Length: 828 bp
ATGGAGTCCCCAAAACCCTCGAGACCTCTTCTCTCCGTCTCCAGCTTCATCCACCACCACTGCCTCCGTCTCGCTGCCGACCTCTCCAACCGCCTCGAAGACACCAAACGGTTCGCCGGTAACCTCCTCCCGCCGGCGACCCGGCGCCTGCTCTCCGCCTCGGGCACTCCGCCTCTGGCTTGGGTGGGCCAGCCGAACCACGCCTTGGCCGCCACCGCGACTCTCAGCTCTGACCACGTGGCGAAGAGCCTGGTCGGCACGGCGGTGTATACTATTAGCAATTCGAACAATGAGTTCGTGCTCATCTCTGACCCCGATCAAGCCAAGTCCATTGGCCTCCTTTGCTTCCGCAAAGAAGACGCCGAAGCCTTCCTCGCTCAGGTTCGGACTCGGACAAGGGAGTTGAGAAGTCCGGCCAAGGTCGTCCCAATCACTCTTGATCAGGTGTACATGCTGAAGGTTGAAGGAATTGCATTTAGGTTTTTGCCTGACCCGGTTCAAATTAAGAATGCACTGGAGCTCAAAGCTGCGGGCAAGAGTGCATTTGATGGAGTTCCTGTTTTTCAGTCCGACCTTCTGGTTGTGAAGAAGAAAAACAAGCGTTACTGCCCAATATATTTCACTAAGGAAGATATAGAAAAGGAATTGTCAAAGGTTTCCAGATCATCCCGAGGACCAGGGGTGTCTCAAAATATTATGGTTGGAAGTTTGGAAGATGTTTTGAGAAAAATGGAGTCAAGTGAAAAGAATTCCGGATGGGAAGATCTGATTTTTATTCCGCCTGGCAAAAGCTACTCCCAACACATTCAAGATGTGGTTAAAAAATGA

Protein Analysis

276

Amino Acids

30.38

Weight (kDa)

9.43

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 10 - 271 2.2e-111 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33350 AT4G33350
fragaria_vesca FvH4_2g34490
malus_domestica MD08G1102400.v1.1 MD15G1083800.v1.1
prunus_persica Prupe.1G436300_v2.0.a1
pyrus_communis pycom08g08320 pycom15g07850 pycom15g07890
rosa_chinensis RchiOBHm_Chr6g0307851
rosa_laevigata RLG00000010693
rosa_multiflora Rmu_sc0003252.1_g000014
rosa_roxburghii Rroxscaffold_7G00160820
rosa_rugosa Rorug06G0361400
rosa_samantha Rh6AG470200 Rh6BG481200 Rh6CG486500 Rh6DG472700
rosa_wichuraiana Rw6G041050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 144
AccB7I CCANNNNNTGG 1 cut(s) 205
AccI GTMKAC 1 cut(s) 269
AccII CGCG 1 cut(s) 218
AccIII TCCGGA 1 cut(s) 752
AcoI YGGCCR 2 cut(s) 207, 414
AcsI RAATTY 1 cut(s) 748
AcuI CTGAAG 1 cut(s) 476
AcvI CACGTG 1 cut(s) 238
AcyI GRCGYC 2 cut(s) 145, 360
AfaI GTAC 1 cut(s) 449
AfiI CCNNNNNNNGG 3 cut(s) 133, 205, 379
AgsI TTSAA 3 cut(s) 464, 500, 809
AjnI CCWGG 3 cut(s) 249, 676, 781
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AleI CACNNNNGTG 1 cut(s) 263
AluBI AGCT 5 cut(s) 45, 228, 520, 527, 792
AluI AGCT 5 cut(s) 45, 228, 520, 527, 792
Alw21I GWGCWC 2 cut(s) 303, 522
Alw26I GTCTC 4 cut(s) 16, 43, 74, 690
Ama87I CYCGRG 3 cut(s) 19, 160, 669
Aor13HI TCCGGA 1 cut(s) 752
AoxI GGCC 4 cut(s) 187, 207, 334, 414
ApeKI GCWGC 2 cut(s) 74, 527
ApoI RAATTY 1 cut(s) 748
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 2 cut(s) 187, 674
AsuC2I CCSGG 2 cut(s) 142, 494
AsuII TTCGAA 1 cut(s) 284
AvaI CYCGRG 3 cut(s) 19, 160, 669
AvaII GGWCC 1 cut(s) 674
BaeGI GKGCMC 1 cut(s) 167
BanI GGYRCC 1 cut(s) 144
BanII GRGCYC 1 cut(s) 522
BbrPI CACGTG 1 cut(s) 238
BbsI GAAGAC 2 cut(s) 105, 363
Bbv12I GWGCWC 2 cut(s) 303, 522
BbvI GCAGC 2 cut(s) 61, 514
BccI CCATC 2 cut(s) 542, 750
BceAI ACGGC 1 cut(s) 276
BciT130I CCWGG 3 cut(s) 251, 678, 783
BclI TGATCA 1 cut(s) 439
BcnI CCSGG 2 cut(s) 142, 494
BcoDI GTCTC 4 cut(s) 16, 43, 74, 690
BfoI RGCGCY 1 cut(s) 148
BglI GCCNNNNNGGC 1 cut(s) 255
BglII AGATCT 1 cut(s) 763
BisI GCNGC 3 cut(s) 75, 210, 528
BlsI GCNGC 3 cut(s) 76, 211, 529
Bme1390I CCNGG 5 cut(s) 142, 251, 494, 678, 783
Bme18I GGWCC 1 cut(s) 674
BmeT110I CYCGRG 3 cut(s) 19, 160, 669
BmgT120I GGNCC 2 cut(s) 187, 674
BmiI GGNNCC 1 cut(s) 146
BmrFI CCNGG 5 cut(s) 142, 251, 494, 678, 783
BpiI GAAGAC 2 cut(s) 105, 363
BpmI CTGGAG 2 cut(s) 25, 536
Bpu10I CCTNAGC 1 cut(s) 378
Bpu14I TTCGAA 1 cut(s) 284
BpuMI CCSGG 2 cut(s) 142, 494
BsaAI YACGTR 1 cut(s) 238
BsaHI GRCGYC 2 cut(s) 145, 360
BsaI GGTCTC 1 cut(s) 16
BsaJI CCNNGG 5 cut(s) 159, 204, 417, 670, 677
BsaWI WCCGGW 1 cut(s) 752
BsaXI ACNNNNNCTCC 2 cut(s) 543, 573
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 205, 379
Bse118I RCCGGY 2 cut(s) 116, 133
Bse1I ACTGG 1 cut(s) 519
BseAI TCCGGA 1 cut(s) 752
BseBI CCWGG 3 cut(s) 251, 678, 783
BseDI CCNNGG 5 cut(s) 159, 204, 417, 670, 677
BseGI GGATG 3 cut(s) 48, 665, 761
BseLI CCNNNNNNNGG 3 cut(s) 133, 205, 379
BseMII CTCAG 2 cut(s) 238, 392
BseNI ACTGG 1 cut(s) 519
BseRI GAGGAG 1 cut(s) 116
BseSI GKGCMC 1 cut(s) 167
BseXI GCAGC 2 cut(s) 61, 514
Bsh1236I CGCG 1 cut(s) 218
BshFI GGCC 4 cut(s) 189, 209, 336, 416
BshNI GGYRCC 1 cut(s) 144
BsiHKAI GWGCWC 2 cut(s) 303, 522
BsiHKCI CYCGRG 3 cut(s) 19, 160, 669
BsiSI CCGG 6 cut(s) 117, 134, 142, 413, 494, 753
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 3 cut(s) 133, 205, 379
BsmAI GTCTC 4 cut(s) 16, 43, 74, 690
BsmBI CGTCTC 2 cut(s) 43, 74
BsmFI GGGAC 1 cut(s) 410
BsmI GAATGC 1 cut(s) 514
BsnI GGCC 4 cut(s) 189, 209, 336, 416
Bso31I GGTCTC 1 cut(s) 16
BsoBI CYCGRG 3 cut(s) 19, 160, 669
Bsp119I TTCGAA 1 cut(s) 284
Bsp1286I GDGCHC 3 cut(s) 167, 303, 522
Bsp13I TCCGGA 1 cut(s) 752
Bsp1407I TGTACA 1 cut(s) 447
Bsp143I GATC 4 cut(s) 316, 439, 662, 763
BspANI GGCC 4 cut(s) 189, 209, 336, 416
BspCNI CTCAG 2 cut(s) 237, 391
BspEI TCCGGA 1 cut(s) 752
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 1 cut(s) 146
BspQI GCTCTTC 1 cut(s) 239
BspT104I TTCGAA 1 cut(s) 284
BspT107I GGYRCC 1 cut(s) 144
BspTNI GGTCTC 1 cut(s) 16
BsrFI RCCGGY 2 cut(s) 116, 133
BsrGI TGTACA 1 cut(s) 447
BsrI ACTGG 1 cut(s) 519
BssAI RCCGGY 2 cut(s) 116, 133
BssECI CCNNGG 5 cut(s) 159, 204, 417, 670, 677
BssMI GATC 4 cut(s) 316, 439, 662, 763
BssNAI GTATAC 1 cut(s) 270
BssNI GRCGYC 2 cut(s) 145, 360
BssT1I CCWWGG 2 cut(s) 204, 417
Bst1107I GTATAC 1 cut(s) 270
Bst2UI CCWGG 3 cut(s) 251, 678, 783
Bst4CI ACNGT 1 cut(s) 111
Bst6I CTCTTC 2 cut(s) 33, 239
BstACI GRCGYC 2 cut(s) 145, 360
BstAUI TGTACA 1 cut(s) 447
BstBAI YACGTR 1 cut(s) 238
BstBI TTCGAA 1 cut(s) 284
BstC8I GCNNGC 4 cut(s) 135, 149, 191, 532
BstDEI CTNAG 3 cut(s) 224, 378, 624
BstEII GGTNACC 1 cut(s) 119
BstENI CCTNNNNNAGG 1 cut(s) 377
BstF5I GGATG 3 cut(s) 48, 665, 761
BstFNI CGCG 1 cut(s) 218
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 4 cut(s) 319, 442, 665, 766
BstMAI GTCTC 4 cut(s) 16, 43, 74, 690
BstMBI GATC 4 cut(s) 316, 439, 662, 763
BstMWI GCNNNNNNNGC 5 cut(s) 209, 215, 255, 342, 374
BstNI CCWGG 3 cut(s) 251, 678, 783
BstNSI RCATGY 1 cut(s) 454
BstPI GGTNACC 1 cut(s) 119
BstSCI CCNGG 5 cut(s) 140, 249, 492, 676, 781
BstSLI GKGCMC 1 cut(s) 167
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 2 cut(s) 61, 514
BstV2I GAAGAC 2 cut(s) 105, 363
BstX2I RGATCY 1 cut(s) 763
BstYI RGATCY 1 cut(s) 763
BstZ17I GTATAC 1 cut(s) 270
BsuRI GGCC 4 cut(s) 189, 209, 336, 416
BtsCI GGATG 3 cut(s) 48, 665, 761
BtsI GCAGTG 1 cut(s) 58
BtsIMutI CAGTG 2 cut(s) 58, 512
Cac8I GCNNGC 4 cut(s) 135, 149, 191, 532
CfoI GCGC 1 cut(s) 147
Cfr10I RCCGGY 2 cut(s) 116, 133
Cfr13I GGNCC 2 cut(s) 187, 674
CseI GACGC 1 cut(s) 368
Csp6I GTAC 1 cut(s) 448
CviAII CATG 1 cut(s) 451
CviQI GTAC 1 cut(s) 448
DdeI CTNAG 3 cut(s) 224, 378, 624
DinI GGCGCC 1 cut(s) 146
DpnI GATC 4 cut(s) 318, 441, 664, 765
DpnII GATC 4 cut(s) 316, 439, 662, 763
EaeI YGGCCR 2 cut(s) 207, 414
Eam1104I CTCTTC 2 cut(s) 33, 239
EarI CTCTTC 2 cut(s) 33, 239
EciI GGCGGA 3 cut(s) 145, 159, 768
Ecl136II GAGCTC 1 cut(s) 520
Eco130I CCWWGG 2 cut(s) 204, 417
Eco24I GRGCYC 1 cut(s) 522
Eco31I GGTCTC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 674
Eco53kI GAGCTC 1 cut(s) 520
Eco57I CTGAAG 1 cut(s) 476
Eco72I CACGTG 1 cut(s) 238
Eco88I CYCGRG 3 cut(s) 19, 160, 669
Eco91I GGTNACC 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 520
EcoNI CCTNNNNNAGG 1 cut(s) 377
EcoO65I GGTNACC 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 748
EcoRII CCWGG 3 cut(s) 249, 676, 781
EcoT14I CCWWGG 2 cut(s) 204, 417
EcoT38I GRGCYC 1 cut(s) 522
EgeI GGCGCC 1 cut(s) 146
EheI GGCGCC 1 cut(s) 146
ErhI CCWWGG 2 cut(s) 204, 417
Esp3I CGTCTC 2 cut(s) 43, 74
FaeI CATG 1 cut(s) 454
FaiI YATR 5 cut(s) 270, 452, 616, 635, 698
FaqI GGGAC 1 cut(s) 410
FatI CATG 1 cut(s) 450
FauI CCCGC 2 cut(s) 138, 523
FbaI TGATCA 1 cut(s) 439
FblI GTMKAC 1 cut(s) 269
Fnu4HI GCNGC 3 cut(s) 75, 210, 528
FokI GGATG 3 cut(s) 35, 652, 768
FriOI GRGCYC 1 cut(s) 522
Fsp4HI GCNGC 3 cut(s) 75, 210, 528
GlaI GCGC 1 cut(s) 146
GluI GCNGC 3 cut(s) 75, 210, 528
GsuI CTGGAG 2 cut(s) 25, 536
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 4 cut(s) 189, 209, 336, 416
HapII CCGG 6 cut(s) 117, 134, 142, 413, 494, 753
HgaI GACGC 1 cut(s) 368
HhaI GCGC 1 cut(s) 147
Hin1I GRCGYC 2 cut(s) 145, 360
Hin1II CATG 1 cut(s) 454
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HinfI GANTC 4 cut(s) 5, 220, 388, 734
HpaII CCGG 6 cut(s) 117, 134, 142, 413, 494, 753
Hpy166II GTNNAC 2 cut(s) 270, 448
Hpy188I TCNGA 6 cut(s) 232, 310, 387, 393, 571, 768
Hpy188III TCNNGA 6 cut(s) 21, 437, 660, 669, 753, 809
Hpy8I GTNNAC 2 cut(s) 270, 448
HpyAV CCTTC 4 cut(s) 379, 451, 458, 584
HpyCH4III ACNGT 1 cut(s) 111
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 3 cut(s) 473, 512, 542
HpyF10VI GCNNNNNNNGC 5 cut(s) 209, 215, 255, 342, 374
HpyF3I CTNAG 3 cut(s) 224, 378, 624
HpySE526I ACGT 1 cut(s) 237
Hsp92I GRCGYC 2 cut(s) 145, 360
Hsp92II CATG 1 cut(s) 454
HspAI GCGC 1 cut(s) 145
KasI GGCGCC 1 cut(s) 144
Kpn2I TCCGGA 1 cut(s) 752
KroI GCCGGC 1 cut(s) 133
KroNI GCCGGC 1 cut(s) 135
Ksp22I TGATCA 1 cut(s) 439
Kzo9I GATC 4 cut(s) 316, 439, 662, 763
LguI GCTCTTC 1 cut(s) 239
LmnI GCTCC 1 cut(s) 517
Lsp1109I GCAGC 2 cut(s) 61, 514
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 2 cut(s) 119, 602
MalI GATC 4 cut(s) 318, 441, 664, 765
MboI GATC 4 cut(s) 316, 439, 662, 763
MboII GAAGA 9 cut(s) 20, 110, 256, 368, 598, 601, 641, 725, 773
MflI RGATCY 1 cut(s) 763
MhlI GDGCHC 3 cut(s) 167, 303, 522
MluCI AATT 5 cut(s) 280, 468, 501, 644, 748
Mly113I GGCGCC 1 cut(s) 145
MlyI GAGTC 4 cut(s) 14, 214, 382, 743
MmeI TCCRAC 3 cut(s) 111, 594, 682
MreI CGCCGGCG 1 cut(s) 133
MroI TCCGGA 1 cut(s) 752
MroNI GCCGGC 1 cut(s) 133
MseI TTAA 2 cut(s) 504, 819
MslI CAYNNNNRTG 1 cut(s) 263
MspI CCGG 6 cut(s) 117, 134, 142, 413, 494, 753
MspR9I CCNGG 5 cut(s) 142, 251, 494, 678, 783
Mva1269I GAATGC 1 cut(s) 514
MvaI CCWGG 3 cut(s) 251, 678, 783
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 5 cut(s) 209, 215, 255, 342, 374
NaeI GCCGGC 1 cut(s) 135
NarI GGCGCC 1 cut(s) 145
NciI CCSGG 2 cut(s) 142, 494
NdeII GATC 4 cut(s) 316, 439, 662, 763
NgoMIV GCCGGC 1 cut(s) 133
NlaIII CATG 1 cut(s) 454
NlaIV GGNNCC 1 cut(s) 146
NspI RCATGY 1 cut(s) 454
NspV TTCGAA 1 cut(s) 284
OliI CACNNNNGTG 1 cut(s) 263
PaeR7I CTCGAG 1 cut(s) 19
PciSI GCTCTTC 1 cut(s) 239
PctI GAATGC 1 cut(s) 514
PdiI GCCGGC 1 cut(s) 135
PflMI CCANNNNNTGG 1 cut(s) 205
PkrI GCNGC 3 cut(s) 76, 211, 529
PleI GAGTC 4 cut(s) 13, 214, 382, 742
PluTI GGCGCC 1 cut(s) 148
PmaCI CACGTG 1 cut(s) 238
PmlI CACGTG 1 cut(s) 238
PpsI GAGTC 4 cut(s) 13, 214, 382, 742
Ppu21I YACGTR 1 cut(s) 238
Psp124BI GAGCTC 1 cut(s) 522
Psp6I CCWGG 3 cut(s) 249, 676, 781
PspCI CACGTG 1 cut(s) 238
PspEI GGTNACC 1 cut(s) 119
PspGI CCWGG 3 cut(s) 249, 676, 781
PspN4I GGNNCC 1 cut(s) 146
PspPI GGNCC 2 cut(s) 187, 674
PsuI RGATCY 1 cut(s) 763
RsaI GTAC 1 cut(s) 449
RsaNI GTAC 1 cut(s) 448
RseI CAYNNNNRTG 1 cut(s) 263
SacI GAGCTC 1 cut(s) 522
SapI GCTCTTC 1 cut(s) 239
SaqAI TTAA 2 cut(s) 504, 819
SatI GCNGC 3 cut(s) 75, 210, 528
Sau3AI GATC 4 cut(s) 316, 439, 662, 763
Sau96I GGNCC 2 cut(s) 187, 674
SchI GAGTC 4 cut(s) 14, 214, 382, 743
ScrFI CCNGG 5 cut(s) 142, 251, 494, 678, 783
SduI GDGCHC 3 cut(s) 167, 303, 522
SfoI GGCGCC 1 cut(s) 146
Sfr274I CTCGAG 1 cut(s) 19
SfuI TTCGAA 1 cut(s) 284
SgrAI CRCCGGYG 1 cut(s) 133
SinI GGWCC 1 cut(s) 674
SlaI CTCGAG 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 19
SmoI CTYRAG 1 cut(s) 19
Sse9I AATT 5 cut(s) 280, 468, 501, 644, 748
SspDI GGCGCC 1 cut(s) 144
SspI AATATT 1 cut(s) 694
SstI GAGCTC 1 cut(s) 522
StyD4I CCNGG 5 cut(s) 140, 249, 492, 676, 781
StyI CCWWGG 2 cut(s) 204, 417
TaaI ACNGT 1 cut(s) 111
TaiI ACGT 1 cut(s) 240
TaqI TCGA 3 cut(s) 20, 96, 284
TasI AATT 5 cut(s) 280, 468, 501, 644, 748
TatI WGTACW 1 cut(s) 447
TauI GCSGC 1 cut(s) 212
Tru1I TTAA 2 cut(s) 504, 819
Tru9I TTAA 2 cut(s) 504, 819
TscAI CASTG 2 cut(s) 65, 519
TseI GCWGC 2 cut(s) 74, 527
TspDTI ATGAA 1 cut(s) 37
TspGWI ACGGA 2 cut(s) 25, 56
TspRI CASTG 2 cut(s) 65, 519
Van91I CCANNNNNTGG 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 674
XagI CCTNNNNNAGG 1 cut(s) 377
XapI RAATTY 1 cut(s) 748
XceI RCATGY 1 cut(s) 454
XhoI CTCGAG 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.