Rh6BG481200

Protein TIC 22, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0012495
Physical Location & Seq
Forward (+)
68689308 .. 68691956
2649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG481200.1

Sequence Viewer

Length: 834 bp
ATGGAGTCCCCAAAACCACCCTTGGGCCCTCTGCTCTCCCTCTCCACCTTCATACACCACCACTGCCTCCGCCTCGCCGCCGACCTCTCCAGCCGCCTCGAAGACACCAAACGCCTCGCAGGAAACTTCCTGCCGCCGGCGACCAGGCGCCTGCTTTCCGCCCCGGGCACTCCGCCGCTGGCTTGGGTGGGCCAGCTGAACCACGCCTTGGCCGCCACCACCGCGACTCTGAGCTCCGACCACGTGGCGAAGAGCCTAGTCGGCACGGCGGTGTATACGGTTAGCAATGCGAACAATGAGTTCGTTCTTATCTCTGACCCCAGTGAAGCCAAGTCCATTGGCCTCCTTTGCTTCCGCAAAGAAGACGCCGAAGCCTTCCTCGCTCAGGTTCGGACTAGGACACGAGAGTTAAGAAGTCCGGTGAAGGTCGTCCCAATCACTCTTGACCAGGTGTACATGCTGAAGGTAGAAGGAATTGCATTTAGGTTTTTGCCTGATCCAGTTCAAATAAAGAATGCACTTGAGCTCAAAGCTGCGGACAGGAGTGCATTTGATGGAGTTCCTGTTTTTCAGTCCGACCTTCTGGTTGTGAAGAAGAAAAACAAGCGTTACTGCCCAATATATTTCACTAAGGAAGATATAGAAAAGGAATTGTCAAAGGTTTCCAGATCATCCCGAGGACCAGGGGTTTCTCAAAATATTATGGTTGGTAGTCTGGAAGATGTTTTGAGAAAAATGGAGTCAAGTGAGAAGAATTCGGGCTGGGAAGATCTGATTTTTATTCCACCTGGCAAAAGCTACTCCCAACACATTCAAGATGTGGTTAAAAAATGA

Protein Analysis

277

Amino Acids

30.59

Weight (kDa)

9.26

Isoelectric Point (pI)

50.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 11 - 273 1.7e-112 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33350 AT4G33350
fragaria_vesca FvH4_2g34490
malus_domestica MD08G1102400.v1.1 MD15G1083800.v1.1
prunus_persica Prupe.1G436300_v2.0.a1
pyrus_communis pycom08g08320 pycom15g07850 pycom15g07890
rosa_chinensis RchiOBHm_Chr6g0307851
rosa_laevigata RLG00000010693
rosa_multiflora Rmu_sc0003252.1_g000014
rosa_roxburghii Rroxscaffold_7G00160820
rosa_rugosa Rorug06G0361400
rosa_samantha Rh6AG470200 Rh6BG481200 Rh6CG486500 Rh6DG472700
rosa_wichuraiana Rw6G041050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 147
AccB7I CCANNNNNTGG 1 cut(s) 208
AccI GTMKAC 1 cut(s) 275
AccII CGCG 1 cut(s) 224
AclWI GGATC 1 cut(s) 491
AcoI YGGCCR 1 cut(s) 210
AcsI RAATTY 1 cut(s) 754
AcuI CTGAAG 1 cut(s) 482
AcvI CACGTG 1 cut(s) 244
AcyI GRCGYC 2 cut(s) 148, 366
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 4 cut(s) 23, 136, 208, 385
AgsI TTSAA 2 cut(s) 506, 815
AjnI CCWGG 4 cut(s) 143, 447, 682, 787
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AleI CACNNNNGTG 1 cut(s) 269
AluBI AGCT 5 cut(s) 196, 234, 526, 533, 798
AluI AGCT 5 cut(s) 196, 234, 526, 533, 798
Alw21I GWGCWC 2 cut(s) 236, 528
AlwI GGATC 1 cut(s) 491
Ama87I CYCGRG 2 cut(s) 163, 675
AoxI GGCC 4 cut(s) 25, 190, 210, 340
ApaI GGGCCC 1 cut(s) 29
ApeKI GCWGC 1 cut(s) 533
ApoI RAATTY 1 cut(s) 754
AspLEI GCGC 1 cut(s) 150
AspS9I GGNCC 4 cut(s) 25, 26, 190, 680
AsuC2I CCSGG 2 cut(s) 164, 165
AsuHPI GGTGA 1 cut(s) 433
AvaI CYCGRG 2 cut(s) 163, 675
AvaII GGWCC 1 cut(s) 680
BaeGI GKGCMC 2 cut(s) 29, 170
BanI GGYRCC 1 cut(s) 147
BanII GRGCYC 3 cut(s) 29, 236, 528
BauI CACGAG 1 cut(s) 402
BbrPI CACGTG 1 cut(s) 244
BbsI GAAGAC 2 cut(s) 108, 369
Bbv12I GWGCWC 2 cut(s) 236, 528
BbvI GCAGC 1 cut(s) 520
BccI CCATC 1 cut(s) 548
BceAI ACGGC 1 cut(s) 282
BciT130I CCWGG 4 cut(s) 145, 449, 684, 789
BcnI CCSGG 2 cut(s) 164, 165
BfaI CTAG 2 cut(s) 257, 396
BfoI RGCGCY 1 cut(s) 151
BglI GCCNNNNNGGC 1 cut(s) 261
BglII AGATCT 1 cut(s) 769
BisI GCNGC 6 cut(s) 78, 94, 134, 176, 213, 534
BlsI GCNGC 6 cut(s) 79, 95, 135, 177, 214, 535
Bme1390I CCNGG 6 cut(s) 145, 164, 165, 449, 684, 789
Bme18I GGWCC 1 cut(s) 680
BmeT110I CYCGRG 2 cut(s) 163, 675
BmgT120I GGNCC 4 cut(s) 25, 26, 190, 680
BmiI GGNNCC 2 cut(s) 27, 149
BmrFI CCNGG 6 cut(s) 145, 164, 165, 449, 684, 789
BmrI ACTGGG 1 cut(s) 315
BmuI ACTGGG 1 cut(s) 315
BpiI GAAGAC 2 cut(s) 108, 369
BpmI CTGGAG 1 cut(s) 73
Bpu10I CCTNAGC 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 542
BpuMI CCSGG 2 cut(s) 164, 165
BsaAI YACGTR 1 cut(s) 244
BsaHI GRCGYC 2 cut(s) 148, 366
BsaJI CCNNGG 6 cut(s) 21, 162, 163, 207, 676, 683
BsaWI WCCGGW 1 cut(s) 418
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 4 cut(s) 23, 136, 208, 385
Bse118I RCCGGY 1 cut(s) 136
Bse1I ACTGG 2 cut(s) 321, 500
Bse3DI GCAATG 1 cut(s) 292
BseBI CCWGG 4 cut(s) 145, 449, 684, 789
BseDI CCNNGG 6 cut(s) 21, 162, 163, 207, 676, 683
BseGI GGATG 1 cut(s) 671
BseLI CCNNNNNNNGG 4 cut(s) 23, 136, 208, 385
BseMI GCAATG 1 cut(s) 292
BseMII CTCAG 2 cut(s) 221, 398
BseNI ACTGG 2 cut(s) 321, 500
BseSI GKGCMC 2 cut(s) 29, 170
BseXI GCAGC 1 cut(s) 520
BseYI CCCAGC 1 cut(s) 762
Bsh1236I CGCG 1 cut(s) 224
BshFI GGCC 4 cut(s) 27, 192, 212, 342
BshNI GGYRCC 1 cut(s) 147
BsiHKAI GWGCWC 2 cut(s) 236, 528
BsiHKCI CYCGRG 2 cut(s) 163, 675
BsiSI CCGG 3 cut(s) 137, 164, 419
BslFI GGGAC 1 cut(s) 416
BslI CCNNNNNNNGG 4 cut(s) 23, 136, 208, 385
BsmFI GGGAC 1 cut(s) 416
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 4 cut(s) 27, 192, 212, 342
BsoBI CYCGRG 2 cut(s) 163, 675
Bsp120I GGGCCC 1 cut(s) 25
Bsp1286I GDGCHC 4 cut(s) 29, 170, 236, 528
Bsp1407I TGTACA 1 cut(s) 453
Bsp143I GATC 3 cut(s) 496, 668, 769
BspANI GGCC 4 cut(s) 27, 192, 212, 342
BspCNI CTCAG 2 cut(s) 222, 397
BspFNI CGCG 1 cut(s) 224
BspLI GGNNCC 2 cut(s) 27, 149
BspPI GGATC 1 cut(s) 491
BspQI GCTCTTC 1 cut(s) 245
BspT107I GGYRCC 1 cut(s) 147
BsrDI GCAATG 1 cut(s) 292
BsrFI RCCGGY 1 cut(s) 136
BsrGI TGTACA 1 cut(s) 453
BsrI ACTGG 2 cut(s) 321, 500
BssAI RCCGGY 1 cut(s) 136
BssECI CCNNGG 6 cut(s) 21, 162, 163, 207, 676, 683
BssMI GATC 3 cut(s) 496, 668, 769
BssNAI GTATAC 1 cut(s) 276
BssNI GRCGYC 2 cut(s) 148, 366
BssSI CACGAG 1 cut(s) 402
BssT1I CCWWGG 2 cut(s) 21, 207
Bst1107I GTATAC 1 cut(s) 276
Bst2BI CACGAG 1 cut(s) 402
Bst2UI CCWGG 4 cut(s) 145, 449, 684, 789
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 1 cut(s) 245
BstACI GRCGYC 2 cut(s) 148, 366
BstAUI TGTACA 1 cut(s) 453
BstBAI YACGTR 1 cut(s) 244
BstC8I GCNNGC 4 cut(s) 138, 152, 180, 194
BstDEI CTNAG 3 cut(s) 230, 384, 630
BstENI CCTNNNNNAGG 1 cut(s) 383
BstF5I GGATG 1 cut(s) 671
BstFNI CGCG 1 cut(s) 224
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 1 cut(s) 150
BstKTI GATC 3 cut(s) 499, 671, 772
BstMBI GATC 3 cut(s) 496, 668, 769
BstMWI GCNNNNNNNGC 5 cut(s) 212, 221, 261, 348, 380
BstNI CCWGG 4 cut(s) 145, 449, 684, 789
BstNSI RCATGY 1 cut(s) 460
BstSCI CCNGG 6 cut(s) 143, 162, 163, 447, 682, 787
BstSLI GKGCMC 2 cut(s) 29, 170
BstUI CGCG 1 cut(s) 224
BstV1I GCAGC 1 cut(s) 520
BstV2I GAAGAC 2 cut(s) 108, 369
BstX2I RGATCY 1 cut(s) 769
BstYI RGATCY 1 cut(s) 769
BstZ17I GTATAC 1 cut(s) 276
BsuRI GGCC 4 cut(s) 27, 192, 212, 342
BtsCI GGATG 1 cut(s) 671
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 2 cut(s) 61, 328
Cac8I GCNNGC 4 cut(s) 138, 152, 180, 194
CfoI GCGC 1 cut(s) 150
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 4 cut(s) 25, 26, 190, 680
Cfr9I CCCGGG 1 cut(s) 163
CseI GACGC 1 cut(s) 374
CsiI ACCWGGT 1 cut(s) 447
Csp6I GTAC 1 cut(s) 454
CviAII CATG 1 cut(s) 457
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 3 cut(s) 230, 384, 630
DinI GGCGCC 1 cut(s) 149
DpnI GATC 3 cut(s) 498, 670, 771
DpnII GATC 3 cut(s) 496, 668, 769
EaeI YGGCCR 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
EciI GGCGGA 3 cut(s) 59, 148, 162
Ecl136II GAGCTC 2 cut(s) 234, 526
Eco130I CCWWGG 2 cut(s) 21, 207
Eco24I GRGCYC 3 cut(s) 29, 236, 528
Eco47I GGWCC 1 cut(s) 680
Eco53kI GAGCTC 2 cut(s) 234, 526
Eco57I CTGAAG 1 cut(s) 482
Eco72I CACGTG 1 cut(s) 244
Eco88I CYCGRG 2 cut(s) 163, 675
EcoICRI GAGCTC 2 cut(s) 234, 526
EcoNI CCTNNNNNAGG 1 cut(s) 383
EcoO109I RGGNCCY 1 cut(s) 26
EcoRI GAATTC 1 cut(s) 754
EcoRII CCWGG 4 cut(s) 143, 447, 682, 787
EcoT14I CCWWGG 2 cut(s) 21, 207
EcoT38I GRGCYC 3 cut(s) 29, 236, 528
EgeI GGCGCC 1 cut(s) 149
EheI GGCGCC 1 cut(s) 149
ErhI CCWWGG 2 cut(s) 21, 207
FaeI CATG 1 cut(s) 460
FaiI YATR 6 cut(s) 53, 276, 458, 622, 641, 704
FaqI GGGAC 1 cut(s) 416
FatI CATG 1 cut(s) 456
FblI GTMKAC 1 cut(s) 275
Fnu4HI GCNGC 6 cut(s) 78, 94, 134, 176, 213, 534
FokI GGATG 1 cut(s) 658
FriOI GRGCYC 3 cut(s) 29, 236, 528
Fsp4HI GCNGC 6 cut(s) 78, 94, 134, 176, 213, 534
FspBI CTAG 2 cut(s) 257, 396
GlaI GCGC 1 cut(s) 149
GluI GCNGC 6 cut(s) 78, 94, 134, 176, 213, 534
GsaI CCCAGC 1 cut(s) 766
GsuI CTGGAG 1 cut(s) 73
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 4 cut(s) 27, 192, 212, 342
HapII CCGG 3 cut(s) 137, 164, 419
HgaI GACGC 1 cut(s) 374
HhaI GCGC 1 cut(s) 150
Hin1I GRCGYC 2 cut(s) 148, 366
Hin1II CATG 1 cut(s) 460
Hin6I GCGC 1 cut(s) 148
HinP1I GCGC 1 cut(s) 148
HinfI GANTC 3 cut(s) 5, 226, 740
HpaII CCGG 3 cut(s) 137, 164, 419
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 2 cut(s) 276, 454
Hpy188I TCNGA 6 cut(s) 231, 238, 316, 393, 577, 774
Hpy188III TCNNGA 5 cut(s) 443, 666, 675, 716, 815
Hpy8I GTNNAC 2 cut(s) 276, 454
HpyAV CCTTC 6 cut(s) 58, 385, 418, 457, 464, 590
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 3 cut(s) 479, 518, 548
HpyF10VI GCNNNNNNNGC 5 cut(s) 212, 221, 261, 348, 380
HpyF3I CTNAG 3 cut(s) 230, 384, 630
HpySE526I ACGT 1 cut(s) 243
Hsp92I GRCGYC 2 cut(s) 148, 366
Hsp92II CATG 1 cut(s) 460
HspAI GCGC 1 cut(s) 148
KasI GGCGCC 1 cut(s) 147
KroI GCCGGC 1 cut(s) 136
KroNI GCCGGC 1 cut(s) 138
Kzo9I GATC 3 cut(s) 496, 668, 769
LguI GCTCTTC 1 cut(s) 245
LmnI GCTCC 1 cut(s) 239
Lsp1109I GCAGC 1 cut(s) 520
MabI ACCWGGT 1 cut(s) 447
MaeI CTAG 2 cut(s) 257, 396
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 1 cut(s) 608
MalI GATC 3 cut(s) 498, 670, 771
MboI GATC 3 cut(s) 496, 668, 769
MboII GAAGA 9 cut(s) 113, 262, 374, 604, 607, 647, 731, 763, 779
MflI RGATCY 1 cut(s) 769
MhlI GDGCHC 4 cut(s) 29, 170, 236, 528
MluCI AATT 3 cut(s) 474, 650, 754
Mly113I GGCGCC 1 cut(s) 148
MlyI GAGTC 3 cut(s) 14, 220, 749
MmeI TCCRAC 2 cut(s) 261, 600
MreI CGCCGGCG 1 cut(s) 136
MroNI GCCGGC 1 cut(s) 136
MseI TTAA 2 cut(s) 410, 825
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 2 cut(s) 178, 196
MspI CCGG 3 cut(s) 137, 164, 419
MspR9I CCNGG 6 cut(s) 145, 164, 165, 449, 684, 789
Mva1269I GAATGC 1 cut(s) 520
MvaI CCWGG 4 cut(s) 145, 449, 684, 789
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 5 cut(s) 212, 221, 261, 348, 380
NaeI GCCGGC 1 cut(s) 138
NarI GGCGCC 1 cut(s) 148
NciI CCSGG 2 cut(s) 164, 165
NdeII GATC 3 cut(s) 496, 668, 769
NgoMIV GCCGGC 1 cut(s) 136
NlaIII CATG 1 cut(s) 460
NlaIV GGNNCC 2 cut(s) 27, 149
NspI RCATGY 1 cut(s) 460
OliI CACNNNNGTG 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 245
PctI GAATGC 1 cut(s) 520
PdiI GCCGGC 1 cut(s) 138
PflMI CCANNNNNTGG 1 cut(s) 208
PkrI GCNGC 6 cut(s) 79, 95, 135, 177, 214, 535
PleI GAGTC 3 cut(s) 13, 220, 748
PluTI GGCGCC 1 cut(s) 151
PmaCI CACGTG 1 cut(s) 244
PmlI CACGTG 1 cut(s) 244
PpsI GAGTC 3 cut(s) 13, 220, 748
Ppu21I YACGTR 1 cut(s) 244
Psp124BI GAGCTC 2 cut(s) 236, 528
Psp6I CCWGG 4 cut(s) 143, 447, 682, 787
PspCI CACGTG 1 cut(s) 244
PspFI CCCAGC 1 cut(s) 762
PspGI CCWGG 4 cut(s) 143, 447, 682, 787
PspN4I GGNNCC 2 cut(s) 27, 149
PspOMI GGGCCC 1 cut(s) 25
PspPI GGNCC 4 cut(s) 25, 26, 190, 680
PsuI RGATCY 1 cut(s) 769
PvuII CAGCTG 1 cut(s) 196
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
RseI CAYNNNNRTG 1 cut(s) 269
SacI GAGCTC 2 cut(s) 236, 528
SapI GCTCTTC 1 cut(s) 245
SaqAI TTAA 2 cut(s) 410, 825
SatI GCNGC 6 cut(s) 78, 94, 134, 176, 213, 534
Sau3AI GATC 3 cut(s) 496, 668, 769
Sau96I GGNCC 4 cut(s) 25, 26, 190, 680
SchI GAGTC 3 cut(s) 14, 220, 749
ScrFI CCNGG 6 cut(s) 145, 164, 165, 449, 684, 789
SduI GDGCHC 4 cut(s) 29, 170, 236, 528
SexAI ACCWGGT 1 cut(s) 447
SfoI GGCGCC 1 cut(s) 149
SgrAI CRCCGGYG 1 cut(s) 136
SinI GGWCC 1 cut(s) 680
SmaI CCCGGG 1 cut(s) 165
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 3 cut(s) 474, 650, 754
SspDI GGCGCC 1 cut(s) 147
SspI AATATT 1 cut(s) 700
SspMI CTAG 2 cut(s) 257, 396
SstI GAGCTC 2 cut(s) 236, 528
StyD4I CCNGG 6 cut(s) 143, 162, 163, 447, 682, 787
StyI CCWWGG 2 cut(s) 21, 207
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 1 cut(s) 246
TaqI TCGA 1 cut(s) 99
TasI AATT 3 cut(s) 474, 650, 754
TatI WGTACW 1 cut(s) 453
TauI GCSGC 5 cut(s) 80, 96, 136, 178, 215
Tru1I TTAA 2 cut(s) 410, 825
Tru9I TTAA 2 cut(s) 410, 825
TscAI CASTG 2 cut(s) 68, 328
TseI GCWGC 1 cut(s) 533
TspDTI ATGAA 1 cut(s) 40
TspMI CCCGGG 1 cut(s) 163
TspRI CASTG 2 cut(s) 68, 328
Van91I CCANNNNNTGG 1 cut(s) 208
VpaK11BI GGWCC 1 cut(s) 680
XagI CCTNNNNNAGG 1 cut(s) 383
XapI RAATTY 1 cut(s) 754
XceI RCATGY 1 cut(s) 460
XmaI CCCGGG 1 cut(s) 163
XmiI GTMKAC 1 cut(s) 275
XspI CTAG 2 cut(s) 257, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.