MD15G1083800.v1.1

Protein TIC 22, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012495
Physical Location & Seq
Reverse (-)
5744127 .. 5747586
3460 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1083800.v1.1.491

Sequence Viewer

Length: 828 bp
ATGGAGTCCGCAAAGCCCTACCCGCGCTCAAACCCTCTGCTCTCGCTCTCCACCTTCATACACCAGCACTGCCTCCGCCTCGGCGCCGAGCTCTCCACCCGTTTCGAAGACACCAAGCGGCTCGCCGGAAACTTCGCGCCGCTGGCGAGCAGGCGCATGTGTTCGGCTCCGGTGTATCCGCCCTTCGCGTGGCTGGCCCAGAGCAGTCACGCCCTGGCCGCTACCCTCAGCTCGGACCACGTGGCGAAGAGCCTGGCCGGCACGGCGGTGTATACGGTTAGCAATTCGAACAATGAGTTCGTGCTCATCTCTGATCCCAATGAGGCCAAGTCCATTGGATTGCTTTGCTTTCGGCAGGAAGACGCCGAAGCATTCCTCGCTCAAGTTCGATCGAGGAGAGAATTACGAAGTGCGGCTAAAGTTGTCCCGATTACTCTTGACCAGGTGTACATGCTGAAGGTTGAAGGAATTGCATTCCGGTTTCTGCCTGACCCAGTTCAAATAAGAAATGCACTCGAGCTCACGTCCGCTGACAGGAGTGCATTTGATGGAGTTCCTGTTTTTCAGTCGGACCTTCTGATTATGAAGAAGAAAAACAAGCGTTACTGCCCAATATATTTCACCAAGGAAGATATAGAAAAAGAATTGTCAAAGGTCTCAAGAGCATCTAGAGGACCAGGGGTTTCTCAACATATTATGGTTGGCAGTTTGGAAGACGTTTTGAGAAAAATGGAGTCAAGCGAAAAGAATTCAGGCTGGGAAGACCTGATTTTCATTCCACCGGGAAAAAGCTACTCCCAACACATTCAAGATGTGGTTAAAGCCTGA

Protein Analysis

276

Amino Acids

30.62

Weight (kDa)

8.97

Isoelectric Point (pI)

55.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 13 - 271 3e-111 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33350 AT4G33350
fragaria_vesca FvH4_2g34490
malus_domestica MD08G1102400.v1.1 MD15G1083800.v1.1
prunus_persica Prupe.1G436300_v2.0.a1
pyrus_communis pycom08g08320 pycom15g07850 pycom15g07890
rosa_chinensis RchiOBHm_Chr6g0307851
rosa_laevigata RLG00000010693
rosa_multiflora Rmu_sc0003252.1_g000014
rosa_roxburghii Rroxscaffold_7G00160820
rosa_rugosa Rorug06G0361400
rosa_samantha Rh6AG470200 Rh6BG481200 Rh6CG486500 Rh6DG472700
rosa_wichuraiana Rw6G041050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 272
AccII CGCG 3 cut(s) 25, 137, 188
AclWI GGATC 1 cut(s) 308
AcoI YGGCCR 2 cut(s) 216, 255
AcsI RAATTY 1 cut(s) 748
AcuI CTGAAG 1 cut(s) 476
AcvI CACGTG 1 cut(s) 241
AcyI GRCGYC 2 cut(s) 84, 363
AfaI GTAC 1 cut(s) 449
AfiI CCNNNNNNNGG 3 cut(s) 189, 232, 534
AgsI TTSAA 3 cut(s) 464, 500, 809
AjiI CACGTC 1 cut(s) 525
AjnI CCWGG 4 cut(s) 213, 252, 441, 676
AleI CACNNNNGTG 1 cut(s) 266
AluBI AGCT 4 cut(s) 91, 231, 520, 792
AluI AGCT 4 cut(s) 91, 231, 520, 792
Alw21I GWGCWC 3 cut(s) 93, 306, 522
Alw26I GTCTC 1 cut(s) 661
AlwI GGATC 1 cut(s) 308
Ama87I CYCGRG 1 cut(s) 515
AoxI GGCC 4 cut(s) 195, 216, 255, 324
ApoI RAATTY 1 cut(s) 748
AspLEI GCGC 4 cut(s) 27, 86, 139, 156
AspS9I GGNCC 4 cut(s) 196, 235, 571, 674
AsuC2I CCSGG 1 cut(s) 783
AsuHPI GGTGA 1 cut(s) 613
AsuII TTCGAA 2 cut(s) 105, 287
AvaI CYCGRG 1 cut(s) 515
AvaII GGWCC 3 cut(s) 235, 571, 674
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 2 cut(s) 93, 522
BarI GAAGNNNNNNTAC 2 cut(s) 167, 199
BbrPI CACGTG 1 cut(s) 241
BbsI GAAGAC 4 cut(s) 114, 366, 720, 768
Bbv12I GWGCWC 3 cut(s) 93, 306, 522
BbvCI CCTCAGC 1 cut(s) 227
BccI CCATC 1 cut(s) 542
BceAI ACGGC 1 cut(s) 279
BciT130I CCWGG 4 cut(s) 215, 254, 443, 678
BciVI GTATCC 1 cut(s) 186
BcnI CCSGG 1 cut(s) 783
BcoDI GTCTC 1 cut(s) 661
BfaI CTAG 1 cut(s) 669
BfoI RGCGCY 1 cut(s) 87
BfuI GTATCC 1 cut(s) 186
BglI GCCNNNNNGGC 2 cut(s) 258, 263
BisI GCNGC 4 cut(s) 119, 140, 219, 414
BlsI GCNGC 4 cut(s) 120, 141, 220, 415
Bme1390I CCNGG 5 cut(s) 215, 254, 443, 678, 783
Bme18I GGWCC 3 cut(s) 235, 571, 674
BmeT110I CYCGRG 1 cut(s) 515
BmgBI CACGTC 1 cut(s) 525
BmgT120I GGNCC 4 cut(s) 196, 235, 571, 674
BmiI GGNNCC 2 cut(s) 85, 168
BmrFI CCNGG 5 cut(s) 215, 254, 443, 678, 783
BmrI ACTGGG 1 cut(s) 488
BmsI GCATC 1 cut(s) 674
BmuI ACTGGG 1 cut(s) 488
BpiI GAAGAC 4 cut(s) 114, 366, 720, 768
Bpu10I CCTNAGC 1 cut(s) 227
Bpu14I TTCGAA 2 cut(s) 105, 287
BpuEI CTTGAG 2 cut(s) 366, 643
BpuMI CCSGG 1 cut(s) 783
BsaAI YACGTR 1 cut(s) 241
BsaHI GRCGYC 2 cut(s) 84, 363
BsaI GGTCTC 1 cut(s) 661
BsaJI CCNNGG 4 cut(s) 79, 213, 624, 677
BsaWI WCCGGW 2 cut(s) 169, 477
BsaXI ACNNNNNCTCC 2 cut(s) 543, 573
Bsc4I CCNNNNNNNGG 3 cut(s) 189, 232, 534
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 1 cut(s) 494
BseBI CCWGG 4 cut(s) 215, 254, 443, 678
BseDI CCNNGG 4 cut(s) 79, 213, 624, 677
BseLI CCNNNNNNNGG 3 cut(s) 189, 232, 534
BseMII CTCAG 1 cut(s) 241
BseNI ACTGG 1 cut(s) 494
BseRI GAGGAG 1 cut(s) 409
BseYI CCCAGC 1 cut(s) 756
Bsh1236I CGCG 3 cut(s) 25, 137, 188
Bsh1285I CGRYCG 1 cut(s) 392
BshFI GGCC 4 cut(s) 197, 218, 257, 326
BshNI GGYRCC 1 cut(s) 83
BsiEI CGRYCG 1 cut(s) 392
BsiHKAI GWGCWC 3 cut(s) 93, 306, 522
BsiHKCI CYCGRG 1 cut(s) 515
BsiSI CCGG 5 cut(s) 126, 170, 258, 478, 782
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 3 cut(s) 189, 232, 534
BsmAI GTCTC 1 cut(s) 661
BsmFI GGGAC 1 cut(s) 410
BsmI GAATGC 2 cut(s) 371, 473
BsnI GGCC 4 cut(s) 197, 218, 257, 326
Bso31I GGTCTC 1 cut(s) 661
BsoBI CYCGRG 1 cut(s) 515
Bsp119I TTCGAA 2 cut(s) 105, 287
Bsp1286I GDGCHC 3 cut(s) 93, 306, 522
Bsp1407I TGTACA 1 cut(s) 447
Bsp143I GATC 2 cut(s) 313, 389
BspANI GGCC 4 cut(s) 197, 218, 257, 326
BspCNI CTCAG 1 cut(s) 240
BspFNI CGCG 3 cut(s) 25, 137, 188
BspLI GGNNCC 2 cut(s) 85, 168
BspPI GGATC 1 cut(s) 308
BspQI GCTCTTC 1 cut(s) 242
BspT104I TTCGAA 2 cut(s) 105, 287
BspT107I GGYRCC 1 cut(s) 83
BspTNI GGTCTC 1 cut(s) 661
BsrFI RCCGGY 1 cut(s) 257
BsrGI TGTACA 1 cut(s) 447
BsrI ACTGG 1 cut(s) 494
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 4 cut(s) 79, 213, 624, 677
BssMI GATC 2 cut(s) 313, 389
BssNAI GTATAC 1 cut(s) 273
BssNI GRCGYC 2 cut(s) 84, 363
BssT1I CCWWGG 1 cut(s) 624
Bst1107I GTATAC 1 cut(s) 273
Bst2UI CCWGG 4 cut(s) 215, 254, 443, 678
Bst4CI ACNGT 1 cut(s) 277
Bst6I CTCTTC 1 cut(s) 242
BstACI GRCGYC 2 cut(s) 84, 363
BstAUI TGTACA 1 cut(s) 447
BstBAI YACGTR 1 cut(s) 241
BstBI TTCGAA 2 cut(s) 105, 287
BstC8I GCNNGC 6 cut(s) 123, 144, 148, 152, 195, 259
BstDEI CTNAG 1 cut(s) 227
BstFNI CGCG 3 cut(s) 25, 137, 188
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 4 cut(s) 27, 86, 139, 156
BstKTI GATC 2 cut(s) 316, 392
BstMAI GTCTC 1 cut(s) 661
BstMBI GATC 2 cut(s) 313, 389
BstMCI CGRYCG 1 cut(s) 392
BstMWI GCNNNNNNNGC 7 cut(s) 22, 143, 194, 218, 258, 263, 377
BstNI CCWGG 4 cut(s) 215, 254, 443, 678
BstNSI RCATGY 2 cut(s) 160, 454
BstSCI CCNGG 5 cut(s) 213, 252, 441, 676, 781
BstUI CGCG 3 cut(s) 25, 137, 188
BstV2I GAAGAC 4 cut(s) 114, 366, 720, 768
BstZ17I GTATAC 1 cut(s) 273
BsuI GTATCC 1 cut(s) 186
BsuRI GGCC 4 cut(s) 197, 218, 257, 326
BtrI CACGTC 1 cut(s) 525
BtsI GCAGTG 1 cut(s) 67
BtsIMutI CAGTG 1 cut(s) 67
Cac8I GCNNGC 6 cut(s) 123, 144, 148, 152, 195, 259
CfoI GCGC 4 cut(s) 27, 86, 139, 156
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 4 cut(s) 196, 235, 571, 674
CseI GACGC 1 cut(s) 371
CsiI ACCWGGT 1 cut(s) 441
Csp6I GTAC 1 cut(s) 448
CviAII CATG 2 cut(s) 157, 451
CviQI GTAC 1 cut(s) 448
DdeI CTNAG 1 cut(s) 227
DinI GGCGCC 1 cut(s) 85
DpnI GATC 2 cut(s) 315, 391
DpnII GATC 2 cut(s) 313, 389
EaeI YGGCCR 2 cut(s) 216, 255
Eam1104I CTCTTC 1 cut(s) 242
EarI CTCTTC 1 cut(s) 242
EciI GGCGGA 2 cut(s) 65, 168
Ecl136II GAGCTC 2 cut(s) 91, 520
Eco130I CCWWGG 1 cut(s) 624
Eco24I GRGCYC 2 cut(s) 93, 522
Eco31I GGTCTC 1 cut(s) 661
Eco47I GGWCC 3 cut(s) 235, 571, 674
Eco53kI GAGCTC 2 cut(s) 91, 520
Eco57I CTGAAG 1 cut(s) 476
Eco72I CACGTG 1 cut(s) 241
Eco88I CYCGRG 1 cut(s) 515
EcoICRI GAGCTC 2 cut(s) 91, 520
EcoRI GAATTC 1 cut(s) 748
EcoRII CCWGG 4 cut(s) 213, 252, 441, 676
EcoT14I CCWWGG 1 cut(s) 624
EcoT38I GRGCYC 2 cut(s) 93, 522
EgeI GGCGCC 1 cut(s) 85
EheI GGCGCC 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 624
FaeI CATG 2 cut(s) 160, 454
FaiI YATR 9 cut(s) 59, 158, 273, 452, 584, 616, 635, 693, 698
FaqI GGGAC 1 cut(s) 410
FatI CATG 2 cut(s) 156, 450
FauI CCCGC 1 cut(s) 30
FblI GTMKAC 1 cut(s) 272
Fnu4HI GCNGC 4 cut(s) 119, 140, 219, 414
FriOI GRGCYC 2 cut(s) 93, 522
Fsp4HI GCNGC 4 cut(s) 119, 140, 219, 414
FspBI CTAG 1 cut(s) 669
GlaI GCGC 4 cut(s) 26, 85, 138, 155
GluI GCNGC 4 cut(s) 119, 140, 219, 414
GsaI CCCAGC 1 cut(s) 760
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 4 cut(s) 197, 218, 257, 326
HapII CCGG 5 cut(s) 126, 170, 258, 478, 782
HgaI GACGC 1 cut(s) 371
HhaI GCGC 4 cut(s) 27, 86, 139, 156
Hin1I GRCGYC 2 cut(s) 84, 363
Hin1II CATG 2 cut(s) 160, 454
Hin6I GCGC 4 cut(s) 25, 84, 137, 154
HinP1I GCGC 4 cut(s) 25, 84, 137, 154
HinfI GANTC 2 cut(s) 5, 734
HpaII CCGG 5 cut(s) 126, 170, 258, 478, 782
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 2 cut(s) 273, 448
Hpy188I TCNGA 4 cut(s) 235, 313, 571, 579
Hpy188III TCNNGA 5 cut(s) 427, 437, 660, 669, 809
Hpy8I GTNNAC 2 cut(s) 273, 448
HpyAV CCTTC 5 cut(s) 64, 193, 451, 458, 584
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4IV ACGT 3 cut(s) 240, 524, 717
HpyCH4V TGCA 3 cut(s) 473, 512, 542
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 143, 194, 218, 258, 263, 377
HpyF3I CTNAG 1 cut(s) 227
HpySE526I ACGT 3 cut(s) 240, 524, 717
Hsp92I GRCGYC 2 cut(s) 84, 363
Hsp92II CATG 2 cut(s) 160, 454
HspAI GCGC 4 cut(s) 25, 84, 137, 154
KasI GGCGCC 1 cut(s) 83
KroI GCCGGC 1 cut(s) 257
KroNI GCCGGC 1 cut(s) 259
Kzo9I GATC 2 cut(s) 313, 389
LguI GCTCTTC 1 cut(s) 242
LmnI GCTCC 1 cut(s) 172
LweI GCATC 1 cut(s) 674
MabI ACCWGGT 1 cut(s) 441
MaeI CTAG 1 cut(s) 669
MaeII ACGT 3 cut(s) 240, 524, 717
MaeIII GTNAC 2 cut(s) 206, 602
MalI GATC 2 cut(s) 315, 391
MboI GATC 2 cut(s) 313, 389
MboII GAAGA 8 cut(s) 119, 259, 371, 598, 601, 641, 725, 773
MhlI GDGCHC 3 cut(s) 93, 306, 522
MluCI AATT 5 cut(s) 283, 401, 468, 644, 748
Mly113I GGCGCC 1 cut(s) 84
MlyI GAGTC 2 cut(s) 14, 743
MmeI TCCRAC 1 cut(s) 549
MnlI CCTC 8 cut(s) 45, 83, 89, 236, 316, 386, 387, 665
MroNI GCCGGC 1 cut(s) 257
MseI TTAA 1 cut(s) 819
MslI CAYNNNNRTG 1 cut(s) 266
MspA1I CMGCKG 2 cut(s) 142, 530
MspI CCGG 5 cut(s) 126, 170, 258, 478, 782
MspR9I CCNGG 5 cut(s) 215, 254, 443, 678, 783
Mva1269I GAATGC 2 cut(s) 371, 473
MvaI CCWGG 4 cut(s) 215, 254, 443, 678
MvnI CGCG 3 cut(s) 25, 137, 188
MwoI GCNNNNNNNGC 7 cut(s) 22, 143, 194, 218, 258, 263, 377
NaeI GCCGGC 1 cut(s) 259
NarI GGCGCC 1 cut(s) 84
NciI CCSGG 1 cut(s) 783
NdeII GATC 2 cut(s) 313, 389
NgoMIV GCCGGC 1 cut(s) 257
NlaIII CATG 2 cut(s) 160, 454
NlaIV GGNNCC 2 cut(s) 85, 168
NmeAIII GCCGAG 2 cut(s) 60, 112
NmuCI GTSAC 1 cut(s) 206
NspI RCATGY 2 cut(s) 160, 454
NspV TTCGAA 2 cut(s) 105, 287
OliI CACNNNNGTG 1 cut(s) 266
PaeR7I CTCGAG 1 cut(s) 515
PciSI GCTCTTC 1 cut(s) 242
PctI GAATGC 2 cut(s) 371, 473
PdiI GCCGGC 1 cut(s) 259
PkrI GCNGC 4 cut(s) 120, 141, 220, 415
Ple19I CGATCG 1 cut(s) 392
PleI GAGTC 2 cut(s) 13, 742
PluTI GGCGCC 1 cut(s) 87
PmaCI CACGTG 1 cut(s) 241
PmlI CACGTG 1 cut(s) 241
PpsI GAGTC 2 cut(s) 13, 742
Ppu21I YACGTR 1 cut(s) 241
Psp124BI GAGCTC 2 cut(s) 93, 522
Psp6I CCWGG 4 cut(s) 213, 252, 441, 676
PspCI CACGTG 1 cut(s) 241
PspFI CCCAGC 1 cut(s) 756
PspGI CCWGG 4 cut(s) 213, 252, 441, 676
PspN4I GGNNCC 2 cut(s) 85, 168
PspPI GGNCC 4 cut(s) 196, 235, 571, 674
PspXI VCTCGAGB 1 cut(s) 515
PvuI CGATCG 1 cut(s) 392
RsaI GTAC 1 cut(s) 449
RsaNI GTAC 1 cut(s) 448
RseI CAYNNNNRTG 1 cut(s) 266
SacI GAGCTC 2 cut(s) 93, 522
SapI GCTCTTC 1 cut(s) 242
SaqAI TTAA 1 cut(s) 819
SatI GCNGC 4 cut(s) 119, 140, 219, 414
Sau3AI GATC 2 cut(s) 313, 389
Sau96I GGNCC 4 cut(s) 196, 235, 571, 674
SchI GAGTC 2 cut(s) 14, 743
ScrFI CCNGG 5 cut(s) 215, 254, 443, 678, 783
SduI GDGCHC 3 cut(s) 93, 306, 522
SexAI ACCWGGT 1 cut(s) 441
SfaNI GCATC 1 cut(s) 674
SfoI GGCGCC 1 cut(s) 85
Sfr274I CTCGAG 1 cut(s) 515
SfuI TTCGAA 2 cut(s) 105, 287
SinI GGWCC 3 cut(s) 235, 571, 674
SlaI CTCGAG 1 cut(s) 515
SmiMI CAYNNNNRTG 1 cut(s) 266
SmlI CTYRAG 3 cut(s) 381, 515, 658
SmoI CTYRAG 3 cut(s) 381, 515, 658
Sse9I AATT 5 cut(s) 283, 401, 468, 644, 748
SspDI GGCGCC 1 cut(s) 83
SspMI CTAG 1 cut(s) 669
SstI GAGCTC 2 cut(s) 93, 522
StyD4I CCNGG 5 cut(s) 213, 252, 441, 676, 781
StyI CCWWGG 1 cut(s) 624
TaaI ACNGT 1 cut(s) 277
TaiI ACGT 3 cut(s) 243, 527, 720
TaqI TCGA 5 cut(s) 105, 287, 388, 392, 516
TasI AATT 5 cut(s) 283, 401, 468, 644, 748
TatI WGTACW 1 cut(s) 447
TauI GCSGC 4 cut(s) 121, 142, 221, 416
Tru1I TTAA 1 cut(s) 819
Tru9I TTAA 1 cut(s) 819
TscAI CASTG 1 cut(s) 74
TseFI GTSAC 1 cut(s) 206
Tsp45I GTSAC 1 cut(s) 206
TspDTI ATGAA 3 cut(s) 46, 599, 763
TspRI CASTG 1 cut(s) 74
VpaK11BI GGWCC 3 cut(s) 235, 571, 674
XapI RAATTY 1 cut(s) 748
XbaI TCTAGA 1 cut(s) 668
XceI RCATGY 2 cut(s) 160, 454
XhoI CTCGAG 1 cut(s) 515
XmiI GTMKAC 1 cut(s) 272
XspI CTAG 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.