AT5G02060

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004667
Physical Location & Seq
Forward (+)
404465 .. 405947
1483 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G02060.1

Sequence Viewer

Length: 459 bp
ATGAAGAAGATGATTGGGAGTCCTGGAACAATGAGTGGGTTGATACTGAGACTAGGGCAATGTGCAACTGCTGCTGCTTCCATTGGTGTTATGGTTTCTTCTTACGACTTTTCCAATTACACTGCTTTCTGCTTCTTAGTCGCATCTATGGGTCTTCAATTGATATGGAGCTTTGGACTTGCGTGTCTTGATGTATATGCGATAAGGAGAAAAAGTGATTTACGAAGCCCTATCTTATTGAGCCTTTTCACTGTTGGTGATTGGGTCACTGCACTTCTCGCTCTTGCAGCCGCGTGTTCCTCTGCAGGAGTTACTGTTTTATTCACTAAAGACACTGAGTTTTGCAGGCAGCAACCCGCTCTCTCTTGCGATAGGTTTCAGATTTCTGTTGGTTTATCTTTCTTTAATTGGTTTTTAGCTGCTATATCGTCTCATACCATGTTTTGGATCTTGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

152

Amino Acids

16.53

Weight (kDa)

8.61

Isoelectric Point (pI)

51.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 7 - 139 4.7e-32 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 183
AccB7I CCANNNNNTGG 1 cut(s) 444
AccBSI CCGCTC 1 cut(s) 359
AccII CGCG 1 cut(s) 293
AciI CCGC 2 cut(s) 291, 357
AclWI GGATC 1 cut(s) 455
AfiI CCNNNNNNNGG 1 cut(s) 444
AgsI TTSAA 1 cut(s) 158
AjnI CCWGG 1 cut(s) 22
AluBI AGCT 2 cut(s) 171, 419
AluI AGCT 2 cut(s) 171, 419
Alw26I GTCTC 2 cut(s) 43, 435
AlwI GGATC 1 cut(s) 455
ApeKI GCWGC 5 cut(s) 71, 74, 287, 349, 419
AsuHPI GGTGA 1 cut(s) 269
BbsI GAAGAC 1 cut(s) 146
BbvI GCAGC 5 cut(s) 58, 61, 299, 361, 406
BciT130I CCWGG 1 cut(s) 24
BcoDI GTCTC 2 cut(s) 43, 435
BfaI CTAG 1 cut(s) 53
BfmI CTRYAG 1 cut(s) 303
BisI GCNGC 6 cut(s) 72, 75, 288, 291, 350, 420
BlsI GCNGC 6 cut(s) 73, 76, 289, 292, 351, 421
Bme1390I CCNGG 1 cut(s) 24
BmrFI CCNGG 1 cut(s) 24
BmsI GCATC 1 cut(s) 152
BpiI GAAGAC 1 cut(s) 146
BsaXI ACNNNNNCTCC 4 cut(s) 199, 229, 300, 330
Bsc4I CCNNNNNNNGG 1 cut(s) 444
Bse3DI GCAATG 1 cut(s) 65
BseBI CCWGG 1 cut(s) 24
BseLI CCNNNNNNNGG 1 cut(s) 444
BseMI GCAATG 1 cut(s) 65
BseMII CTCAG 2 cut(s) 38, 327
BseXI GCAGC 5 cut(s) 58, 61, 299, 361, 406
BsgI GTGCAG 1 cut(s) 255
Bsh1236I CGCG 1 cut(s) 293
BslI CCNNNNNNNGG 1 cut(s) 444
BsmAI GTCTC 2 cut(s) 43, 435
BsmBI CGTCTC 1 cut(s) 435
Bsp143I GATC 1 cut(s) 447
BspACI CCGC 2 cut(s) 291, 357
BspCNI CTCAG 2 cut(s) 39, 328
BspFNI CGCG 1 cut(s) 293
BspMAI CTGCAG 1 cut(s) 307
BspPI GGATC 1 cut(s) 455
BsrBI CCGCTC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 65
BssMI GATC 1 cut(s) 447
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 2 cut(s) 253, 316
BstAPI GCANNNNNTGC 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 347
BstDEI CTNAG 3 cut(s) 47, 136, 336
BstFNI CGCG 1 cut(s) 293
BstKTI GATC 1 cut(s) 450
BstMAI GTCTC 2 cut(s) 43, 435
BstMBI GATC 1 cut(s) 447
BstMWI GCNNNNNNNGC 3 cut(s) 71, 278, 287
BstNI CCWGG 1 cut(s) 24
BstSCI CCNGG 1 cut(s) 22
BstSFI CTRYAG 1 cut(s) 303
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 5 cut(s) 58, 61, 299, 361, 406
BstV2I GAAGAC 1 cut(s) 146
BstX2I RGATCY 1 cut(s) 447
BstYI RGATCY 1 cut(s) 447
BtsI GCAGTG 2 cut(s) 120, 267
BtsIMutI CAGTG 4 cut(s) 120, 249, 267, 333
Cac8I GCNNGC 1 cut(s) 347
CviAII CATG 1 cut(s) 439
CviJI RGCY 5 cut(s) 171, 228, 243, 290, 419
CviKI_1 RGCY 5 cut(s) 171, 228, 243, 290, 419
DdeI CTNAG 3 cut(s) 47, 136, 336
DpnI GATC 1 cut(s) 449
DpnII GATC 1 cut(s) 447
DrdI GACNNNNNNGTC 1 cut(s) 183
DseDI GACNNNNNNGTC 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 22
Esp3I CGTCTC 1 cut(s) 435
FaeI CATG 1 cut(s) 442
FaiI YATR 9 cut(s) 92, 149, 166, 196, 198, 425, 435, 440, 457
FatI CATG 1 cut(s) 438
FauI CCCGC 1 cut(s) 364
Fnu4HI GCNGC 6 cut(s) 72, 75, 288, 291, 350, 420
Fsp4HI GCNGC 6 cut(s) 72, 75, 288, 291, 350, 420
FspBI CTAG 1 cut(s) 53
GluI GCNGC 6 cut(s) 72, 75, 288, 291, 350, 420
Hin1II CATG 1 cut(s) 442
HinfI GANTC 1 cut(s) 19
HphI GGTGA 1 cut(s) 269
Hpy188I TCNGA 1 cut(s) 381
Hpy188III TCNNGA 2 cut(s) 188, 451
HpyCH4III ACNGT 2 cut(s) 253, 316
HpyCH4V TGCA 5 cut(s) 65, 272, 287, 305, 345
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 278, 287
HpyF3I CTNAG 3 cut(s) 47, 136, 336
Hsp92II CATG 1 cut(s) 442
Kzo9I GATC 1 cut(s) 447
LmnI GCTCC 1 cut(s) 168
LpnPI CCDG 4 cut(s) 9, 36, 291, 331
Lsp1109I GCAGC 5 cut(s) 58, 61, 299, 361, 406
LweI GCATC 1 cut(s) 152
MaeI CTAG 1 cut(s) 53
MaeIII GTNAC 2 cut(s) 265, 310
MalI GATC 1 cut(s) 449
MbiI CCGCTC 1 cut(s) 359
MboI GATC 1 cut(s) 447
MboII GAAGA 4 cut(s) 16, 19, 90, 146
MfeI CAATTG 1 cut(s) 158
MflI RGATCY 1 cut(s) 447
MluCI AATT 3 cut(s) 115, 158, 406
MlyI GAGTC 1 cut(s) 28
MnlI CCTC 1 cut(s) 310
MseI TTAA 1 cut(s) 405
MspR9I CCNGG 1 cut(s) 24
MunI CAATTG 1 cut(s) 158
MvaI CCWGG 1 cut(s) 24
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 3 cut(s) 71, 278, 287
NdeII GATC 1 cut(s) 447
NlaIII CATG 1 cut(s) 442
NmuCI GTSAC 1 cut(s) 265
PflMI CCANNNNNTGG 1 cut(s) 444
PfoI TCCNGGA 1 cut(s) 22
PkrI GCNGC 6 cut(s) 73, 76, 289, 292, 351, 421
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 22
PspGI CCWGG 1 cut(s) 22
PstI CTGCAG 1 cut(s) 307
PsuI RGATCY 1 cut(s) 447
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 6 cut(s) 72, 75, 288, 291, 350, 420
Sau3AI GATC 1 cut(s) 447
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 1 cut(s) 24
SetI ASST 3 cut(s) 173, 377, 421
SfaNI GCATC 1 cut(s) 152
SfcI CTRYAG 1 cut(s) 303
Sse9I AATT 3 cut(s) 115, 158, 406
SsiI CCGC 2 cut(s) 291, 357
SspMI CTAG 1 cut(s) 53
StyD4I CCNGG 1 cut(s) 22
TaaI ACNGT 2 cut(s) 253, 316
TasI AATT 3 cut(s) 115, 158, 406
TauI GCSGC 1 cut(s) 293
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 4 cut(s) 127, 256, 274, 340
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 5 cut(s) 71, 74, 287, 349, 419
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 4 cut(s) 127, 256, 274, 340
Van91I CCANNNNNTGG 1 cut(s) 444
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.