MD01G1140000.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004667
Physical Location & Seq
Reverse (-)
24948201 .. 24951428
3228 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1140000.v1.1.491

Sequence Viewer

Length: 462 bp
ATGAAGAAGCTGTTTGGGAGCCCAGGGAAGGTGAGTGGGCTGGCACTGAGAATTGGGCAGTGCTGTTTTGCTGCTGCTGCTATTGGGGTGATGCTCTCTGCTCATGGTTTCTTCAATGCCACTGCATTTTGTTATTTGATTGCATCAATGGGGCTTCAAGTTGTTTGGAGTTTTGGACTTGCTTGCCTTGATTTACATGCTTTGAGGTCAAAGAGAAGCTTGCAGAATCCTATTTTAGTGAGCCTATTTGTTGTCGGGGATTGGGTGACGTCTACGCTGTCATTGGCGGCTGCATGCTCATCAGCGGGGGTGACAGTTCTGTACTCAAAAGATTTGAATTACTGCAGGCCAATGCATCTTCCGTGCAGCAAGTTCCAAATCGCCGTTGCCTTCGCCTTCATCTCATGGTTTCTCCTCGCTGTTTCGTCAATTGTCATGTTTCGGTTGTTAGGCGCAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

154

Amino Acids

16.36

Weight (kDa)

9.43

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 7 - 138 2e-27 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 272
AccI GTMKAC 1 cut(s) 272
AciI CCGC 2 cut(s) 287, 305
AcyI GRCGYC 1 cut(s) 269
AfaI GTAC 1 cut(s) 323
AfiI CCNNNNNNNGG 1 cut(s) 28
AgsI TTSAA 3 cut(s) 115, 158, 337
AjnI CCWGG 1 cut(s) 22
AluBI AGCT 2 cut(s) 10, 219
AluI AGCT 2 cut(s) 10, 219
AoxI GGCC 1 cut(s) 347
ApeKI GCWGC 5 cut(s) 71, 74, 77, 290, 366
AspLEI GCGC 1 cut(s) 455
AsuHPI GGTGA 4 cut(s) 43, 100, 277, 322
BanII GRGCYC 1 cut(s) 23
BbvI GCAGC 5 cut(s) 58, 61, 64, 277, 378
BceAI ACGGC 1 cut(s) 368
BciT130I CCWGG 1 cut(s) 24
BfmI CTRYAG 1 cut(s) 343
BisI GCNGC 6 cut(s) 72, 75, 78, 288, 291, 367
BlsI GCNGC 6 cut(s) 73, 76, 79, 289, 292, 368
Bme1390I CCNGG 1 cut(s) 24
BmiI GGNNCC 1 cut(s) 20
BmrFI CCNGG 1 cut(s) 24
BmsI GCATC 3 cut(s) 81, 152, 364
BsaHI GRCGYC 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 22, 23
Bsc4I CCNNNNNNNGG 1 cut(s) 28
BseBI CCWGG 1 cut(s) 24
BseDI CCNNGG 2 cut(s) 22, 23
BseLI CCNNNNNNNGG 1 cut(s) 28
BseMII CTCAG 1 cut(s) 38
BseRI GAGGAG 1 cut(s) 404
BseXI GCAGC 5 cut(s) 58, 61, 64, 277, 378
BsgI GTGCAG 1 cut(s) 385
BshFI GGCC 1 cut(s) 349
BslI CCNNNNNNNGG 1 cut(s) 28
BsnI GGCC 1 cut(s) 349
Bsp1286I GDGCHC 1 cut(s) 23
BspACI CCGC 2 cut(s) 287, 305
BspANI GGCC 1 cut(s) 349
BspCNI CTCAG 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 20
BspMAI CTGCAG 1 cut(s) 347
BssECI CCNNGG 2 cut(s) 22, 23
BssNI GRCGYC 1 cut(s) 269
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 1 cut(s) 316
BstACI GRCGYC 1 cut(s) 269
BstC8I GCNNGC 5 cut(s) 42, 184, 221, 295, 347
BstDEI CTNAG 1 cut(s) 47
BstHHI GCGC 1 cut(s) 455
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstNI CCWGG 1 cut(s) 24
BstNSI RCATGY 2 cut(s) 200, 297
BstSCI CCNGG 1 cut(s) 22
BstSFI CTRYAG 1 cut(s) 343
BstV1I GCAGC 5 cut(s) 58, 61, 64, 277, 378
BsuRI GGCC 1 cut(s) 349
BtsI GCAGTG 2 cut(s) 65, 120
BtsIMutI CAGTG 3 cut(s) 44, 65, 120
Cac8I GCNNGC 5 cut(s) 42, 184, 221, 295, 347
CfoI GCGC 1 cut(s) 455
Csp6I GTAC 1 cut(s) 322
CviAII CATG 5 cut(s) 104, 197, 294, 405, 436
CviJI RGCY 8 cut(s) 10, 21, 40, 154, 219, 243, 290, 349
CviKI_1 RGCY 8 cut(s) 10, 21, 40, 154, 219, 243, 290, 349
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 1 cut(s) 47
Eco24I GRGCYC 1 cut(s) 23
EcoRII CCWGG 1 cut(s) 22
EcoT22I ATGCAT 1 cut(s) 357
EcoT38I GRGCYC 1 cut(s) 23
FaeI CATG 5 cut(s) 107, 200, 297, 408, 439
FaiI YATR 6 cut(s) 105, 198, 295, 406, 437, 460
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FatI CATG 5 cut(s) 103, 196, 293, 404, 435
FauI CCCGC 1 cut(s) 298
FblI GTMKAC 1 cut(s) 272
Fnu4HI GCNGC 6 cut(s) 72, 75, 78, 288, 291, 367
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 6 cut(s) 72, 75, 78, 288, 291, 367
GlaI GCGC 1 cut(s) 454
GluI GCNGC 6 cut(s) 72, 75, 78, 288, 291, 367
HaeIII GGCC 1 cut(s) 349
HhaI GCGC 1 cut(s) 455
Hin1I GRCGYC 1 cut(s) 269
Hin1II CATG 5 cut(s) 107, 200, 297, 408, 439
Hin6I GCGC 1 cut(s) 453
HinP1I GCGC 1 cut(s) 453
HindIII AAGCTT 1 cut(s) 217
HinfI GANTC 1 cut(s) 226
HphI GGTGA 4 cut(s) 43, 100, 277, 322
Hpy166II GTNNAC 1 cut(s) 273
Hpy8I GTNNAC 1 cut(s) 273
HpyAV CCTTC 3 cut(s) 22, 400, 406
HpyCH4III ACNGT 1 cut(s) 316
HpyCH4IV ACGT 1 cut(s) 269
HpyCH4V TGCA 7 cut(s) 125, 143, 223, 293, 345, 355, 366
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpyF3I CTNAG 1 cut(s) 47
HpySE526I ACGT 1 cut(s) 269
Hsp92I GRCGYC 1 cut(s) 269
Hsp92II CATG 5 cut(s) 107, 200, 297, 408, 439
HspAI GCGC 1 cut(s) 453
LmnI GCTCC 1 cut(s) 18
LpnPI CCDG 4 cut(s) 9, 26, 36, 331
Lsp1109I GCAGC 5 cut(s) 58, 61, 64, 277, 378
LweI GCATC 3 cut(s) 81, 152, 364
MaeII ACGT 1 cut(s) 269
MaeIII GTNAC 2 cut(s) 265, 310
MboII GAAGA 3 cut(s) 16, 103, 350
MfeI CAATTG 1 cut(s) 429
MhlI GDGCHC 1 cut(s) 23
MluCI AATT 3 cut(s) 51, 337, 429
MnlI CCTC 2 cut(s) 198, 425
Mph1103I ATGCAT 1 cut(s) 357
MspA1I CMGCKG 1 cut(s) 305
MspR9I CCNGG 1 cut(s) 24
MunI CAATTG 1 cut(s) 429
MvaI CCWGG 1 cut(s) 24
MwoI GCNNNNNNNGC 1 cut(s) 77
NlaIII CATG 5 cut(s) 107, 200, 297, 408, 439
NlaIV GGNNCC 1 cut(s) 20
NmuCI GTSAC 2 cut(s) 265, 310
NsiI ATGCAT 1 cut(s) 357
NspI RCATGY 2 cut(s) 200, 297
PaeI GCATGC 1 cut(s) 297
PasI CCCWGGG 1 cut(s) 23
PfeI GAWTC 1 cut(s) 226
PkrI GCNGC 6 cut(s) 73, 76, 79, 289, 292, 368
Psp6I CCWGG 1 cut(s) 22
PspGI CCWGG 1 cut(s) 22
PspN4I GGNNCC 1 cut(s) 20
PstI CTGCAG 1 cut(s) 347
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
SatI GCNGC 6 cut(s) 72, 75, 78, 288, 291, 367
ScrFI CCNGG 1 cut(s) 24
SduI GDGCHC 1 cut(s) 23
SetI ASST 5 cut(s) 12, 33, 209, 221, 272
SfaNI GCATC 3 cut(s) 81, 152, 364
SfcI CTRYAG 1 cut(s) 343
SphI GCATGC 1 cut(s) 297
Sse9I AATT 3 cut(s) 51, 337, 429
SsiI CCGC 2 cut(s) 287, 305
StyD4I CCNGG 1 cut(s) 22
TaaI ACNGT 1 cut(s) 316
TaiI ACGT 1 cut(s) 272
TasI AATT 3 cut(s) 51, 337, 429
TatI WGTACW 1 cut(s) 321
TauI GCSGC 1 cut(s) 290
TfiI GAWTC 1 cut(s) 226
TscAI CASTG 3 cut(s) 51, 65, 127
TseFI GTSAC 2 cut(s) 265, 310
TseI GCWGC 5 cut(s) 71, 74, 77, 290, 366
Tsp45I GTSAC 2 cut(s) 265, 310
TspDTI ATGAA 2 cut(s) 17, 388
TspGWI ACGGA 1 cut(s) 351
TspRI CASTG 3 cut(s) 51, 65, 127
XceI RCATGY 2 cut(s) 200, 297
XmiI GTMKAC 1 cut(s) 272
ZraI GACGTC 1 cut(s) 270
Zsp2I ATGCAT 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.