FvH4_6g13050

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004667
Physical Location & Seq
Forward (+)
7949048 .. 7951180
2133 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g13050.t1

Sequence Viewer

Length: 468 bp
ATGAAGGAGTTGATAGGTAGACCAGGGTCGGTTACTGGGTTACTACTCAGAATCGGCCAATTCGGGTTCGCTTCCGCCGCCATCGGAGTTATGCTCTCCGCCGATCGCTTCGCCACATTCACCGCCTTCTGCTATTTGATTGCGTCAATGGGGCTTCAAGTGCTATGGAGCTTTGGGCTTGCTTGTCTTGATATTTATGCGTTGAGGAGAAAGAGAGACCTCCAAAATCCAATCCTAGTTAGCCTATTTGTTGTCGGTGATTGGGTAACAGCTACTTTATCACTTGCTGCTGCAAGCGCTTCAGCCGGAATAGTAATCCTTTATTCCAAGGACACTGACTTCTGCACTTCAAAATGGGATATACCATGCCAGAAGTTCCAGATATCGGTGGCTTTTGCTTTCCTTACCTGGGCCTTTATTGCCATATCTTCTCATGTGATGTTTTGGATCCTATTAGCTTCGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

156

Amino Acids

16.92

Weight (kDa)

8.8

Isoelectric Point (pI)

28.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 7 - 139 6.4e-30 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 19
AciI CCGC 4 cut(s) 75, 78, 99, 123
AclWI GGATC 2 cut(s) 442, 455
AcoI YGGCCR 1 cut(s) 55
AcuI CTGAAG 1 cut(s) 285
AfeI AGCGCT 1 cut(s) 298
AfiI CCNNNNNNNGG 2 cut(s) 385, 409
AgsI TTSAA 2 cut(s) 158, 351
AjnI CCWGG 2 cut(s) 22, 407
AluBI AGCT 3 cut(s) 171, 272, 458
AluI AGCT 3 cut(s) 171, 272, 458
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 2 cut(s) 442, 455
Aor51HI AGCGCT 1 cut(s) 298
AoxI GGCC 2 cut(s) 55, 411
ApeKI GCWGC 2 cut(s) 287, 290
AspLEI GCGC 1 cut(s) 299
AspS9I GGNCC 1 cut(s) 411
AsuHPI GGTGA 2 cut(s) 112, 269
BamHI GGATCC 1 cut(s) 447
BbvI GCAGC 2 cut(s) 274, 277
BccI CCATC 1 cut(s) 89
BciT130I CCWGG 2 cut(s) 24, 409
BcoDI GTCTC 1 cut(s) 210
BfaI CTAG 1 cut(s) 236
BfoI RGCGCY 1 cut(s) 300
BisI GCNGC 3 cut(s) 78, 288, 291
BlsI GCNGC 3 cut(s) 79, 289, 292
Bme1390I CCNGG 2 cut(s) 24, 409
BmgT120I GGNCC 1 cut(s) 411
BmiI GGNNCC 1 cut(s) 449
BmrFI CCNGG 2 cut(s) 24, 409
BmrI ACTGGG 1 cut(s) 45
BmuI ACTGGG 1 cut(s) 45
BoxI GACNNNNGTC 1 cut(s) 25
BplI GAGNNNNNCTC 2 cut(s) 78, 110
BsaI GGTCTC 1 cut(s) 210
BsaJI CCNNGG 3 cut(s) 23, 327, 408
Bsc4I CCNNNNNNNGG 2 cut(s) 385, 409
Bse1I ACTGG 1 cut(s) 40
BseBI CCWGG 2 cut(s) 24, 409
BseDI CCNNGG 3 cut(s) 23, 327, 408
BseLI CCNNNNNNNGG 2 cut(s) 385, 409
BseMII CTCAG 1 cut(s) 61
BseNI ACTGG 1 cut(s) 40
BseRI GAGGAG 1 cut(s) 220
BseXI GCAGC 2 cut(s) 274, 277
BsgI GTGCAG 1 cut(s) 328
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 2 cut(s) 57, 413
BsiEI CGRYCG 1 cut(s) 106
BsiSI CCGG 1 cut(s) 306
BslI CCNNNNNNNGG 2 cut(s) 385, 409
BsmAI GTCTC 1 cut(s) 210
BsnI GGCC 2 cut(s) 57, 413
Bso31I GGTCTC 1 cut(s) 210
Bsp143I GATC 2 cut(s) 103, 447
BspACI CCGC 4 cut(s) 75, 78, 99, 123
BspANI GGCC 2 cut(s) 57, 413
BspCNI CTCAG 1 cut(s) 60
BspLI GGNNCC 1 cut(s) 449
BspPI GGATC 2 cut(s) 442, 455
BspTNI GGTCTC 1 cut(s) 210
BsrI ACTGG 1 cut(s) 40
BssECI CCNNGG 3 cut(s) 23, 327, 408
BssMI GATC 2 cut(s) 103, 447
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 2 cut(s) 24, 409
BstC8I GCNNGC 2 cut(s) 180, 295
BstDEI CTNAG 1 cut(s) 47
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 2 cut(s) 106, 450
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 2 cut(s) 103, 447
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 4 cut(s) 77, 160, 296, 419
BstNI CCWGG 2 cut(s) 24, 409
BstPAI GACNNNNGTC 1 cut(s) 25
BstSCI CCNGG 2 cut(s) 22, 407
BstV1I GCAGC 2 cut(s) 274, 277
BstX2I RGATCY 1 cut(s) 447
BstYI RGATCY 1 cut(s) 447
BsuRI GGCC 2 cut(s) 57, 413
BtsIMutI CAGTG 1 cut(s) 333
Cac8I GCNNGC 2 cut(s) 180, 295
CfoI GCGC 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 411
CseI GACGC 1 cut(s) 132
CviAII CATG 2 cut(s) 366, 434
DdeI CTNAG 1 cut(s) 47
DpnI GATC 2 cut(s) 105, 449
DpnII GATC 2 cut(s) 103, 447
EaeI YGGCCR 1 cut(s) 55
EciI GGCGGA 2 cut(s) 64, 88
Eco130I CCWWGG 1 cut(s) 327
Eco31I GGTCTC 1 cut(s) 210
Eco32I GATATC 1 cut(s) 384
Eco47III AGCGCT 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 285
EcoRII CCWGG 2 cut(s) 22, 407
EcoRV GATATC 1 cut(s) 384
EcoT14I CCWWGG 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 2 cut(s) 369, 437
FaiI YATR 7 cut(s) 92, 166, 198, 362, 367, 425, 435
FatI CATG 2 cut(s) 365, 433
FblI GTMKAC 1 cut(s) 19
Fnu4HI GCNGC 3 cut(s) 78, 288, 291
Fsp4HI GCNGC 3 cut(s) 78, 288, 291
FspBI CTAG 1 cut(s) 236
GlaI GCGC 1 cut(s) 298
GluI GCNGC 3 cut(s) 78, 288, 291
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 2 cut(s) 57, 413
HapII CCGG 1 cut(s) 306
HgaI GACGC 1 cut(s) 132
HhaI GCGC 1 cut(s) 299
Hin1II CATG 2 cut(s) 369, 437
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HinfI GANTC 1 cut(s) 51
HpaII CCGG 1 cut(s) 306
HphI GGTGA 2 cut(s) 112, 269
Hpy166II GTNNAC 1 cut(s) 20
Hpy188I TCNGA 2 cut(s) 50, 86
Hpy188III TCNNGA 2 cut(s) 188, 379
Hpy8I GTNNAC 1 cut(s) 20
HpyAV CCTTC 1 cut(s) 136
HpyCH4V TGCA 2 cut(s) 293, 345
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 160, 296, 419
HpyF3I CTNAG 1 cut(s) 47
Hsp92II CATG 2 cut(s) 369, 437
HspAI GCGC 1 cut(s) 297
Kzo9I GATC 2 cut(s) 103, 447
LmnI GCTCC 1 cut(s) 168
LpnPI CCDG 8 cut(s) 9, 21, 36, 319, 383, 392, 394, 421
Lsp1109I GCAGC 2 cut(s) 274, 277
MaeI CTAG 1 cut(s) 236
MaeIII GTNAC 3 cut(s) 31, 39, 265
MalI GATC 2 cut(s) 105, 449
MboI GATC 2 cut(s) 103, 447
MboII GAAGA 1 cut(s) 420
MflI RGATCY 1 cut(s) 447
MluCI AATT 1 cut(s) 59
MnlI CCTC 2 cut(s) 198, 230
MspI CCGG 1 cut(s) 306
MspR9I CCNGG 2 cut(s) 24, 409
MvaI CCWGG 2 cut(s) 24, 409
MwoI GCNNNNNNNGC 4 cut(s) 77, 160, 296, 419
NdeII GATC 2 cut(s) 103, 447
NlaIII CATG 2 cut(s) 369, 437
NlaIV GGNNCC 1 cut(s) 449
PfeI GAWTC 1 cut(s) 51
PkrI GCNGC 3 cut(s) 79, 289, 292
Ple19I CGATCG 1 cut(s) 106
PshAI GACNNNNGTC 1 cut(s) 25
Psp6I CCWGG 2 cut(s) 22, 407
PspGI CCWGG 2 cut(s) 22, 407
PspN4I GGNNCC 1 cut(s) 449
PspPI GGNCC 1 cut(s) 411
PsuI RGATCY 1 cut(s) 447
PvuI CGATCG 1 cut(s) 106
SatI GCNGC 3 cut(s) 78, 288, 291
Sau3AI GATC 2 cut(s) 103, 447
Sau96I GGNCC 1 cut(s) 411
ScrFI CCNGG 2 cut(s) 24, 409
SetI ASST 6 cut(s) 19, 173, 222, 274, 410, 460
Sse9I AATT 1 cut(s) 59
SsiI CCGC 4 cut(s) 75, 78, 99, 123
SspMI CTAG 1 cut(s) 236
StyD4I CCNGG 2 cut(s) 22, 407
StyI CCWWGG 1 cut(s) 327
TasI AATT 1 cut(s) 59
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 1 cut(s) 51
TscAI CASTG 1 cut(s) 340
TseI GCWGC 2 cut(s) 287, 290
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 340
XmiI GTMKAC 1 cut(s) 19
XspI CTAG 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.