MD04G1136000.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004667
Physical Location & Seq
Reverse (-)
22307041 .. 22310991
3951 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1136000.v1.1.491

Sequence Viewer

Length: 468 bp
ATGAAGGAGCTGCTCGGGAGACCTGGGTCTGTGAGCGGGTTGCTGCTGAGGGTATCGCAATTTTCATGTGCTGGTATCGCCATCGCCGTCATGGTCACCACCGACCGCTTCGCTGAATTCACCTGTTTCTGCTATTTGGTTTCGTCGATGGCACTTCAAGTGCTATGGAGCTTGGGGCTTGCTTGTCTTGATATTTATGCTTTGAGCAAAAAGAGAGACCTTCAAAATAAATTACTAGTGAGGCTATTTGTTGTCGGTGATTGGGTAACAGCTACTTTATCACTGGCTGCTGCAAGCTCTTCATCAGGGATTGCAATTCTGTTTGTAAGAGACTTTCATTTCTGCACAGGTAGATGGGGTTTACCATGTGGTAGCTACATGCTTGCAGTTTCCTTCGCTTTTGTCACCTGGATCCTTACTTCCATATCCTCTCACGTGATGTTTTGGATCCTATCGGCATTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

156

Amino Acids

16.95

Weight (kDa)

8.86

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 7 - 139 5.2e-25 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 36
AciI CCGC 2 cut(s) 36, 106
AclWI GGATC 4 cut(s) 406, 419, 442, 455
AcsI RAATTY 1 cut(s) 116
AcvI CACGTG 1 cut(s) 436
AgsI TTSAA 2 cut(s) 158, 224
AhlI ACTAGT 1 cut(s) 235
AjnI CCWGG 2 cut(s) 22, 407
AluBI AGCT 5 cut(s) 10, 171, 272, 297, 375
AluI AGCT 5 cut(s) 10, 171, 272, 297, 375
Alw26I GTCTC 3 cut(s) 13, 210, 324
AlwI GGATC 4 cut(s) 406, 419, 442, 455
Ama87I CYCGRG 1 cut(s) 14
ApeKI GCWGC 4 cut(s) 10, 43, 287, 290
ApoI RAATTY 1 cut(s) 116
AsuHPI GGTGA 4 cut(s) 88, 112, 269, 397
AvaI CYCGRG 1 cut(s) 14
BamHI GGATCC 2 cut(s) 411, 447
BbrPI CACGTG 1 cut(s) 436
BbvCI CCTCAGC 1 cut(s) 47
BbvI GCAGC 3 cut(s) 30, 274, 277
BccI CCATC 3 cut(s) 89, 142, 348
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 2 cut(s) 24, 409
BcoDI GTCTC 3 cut(s) 13, 210, 324
BcuI ACTAGT 1 cut(s) 235
BfaI CTAG 1 cut(s) 236
BisI GCNGC 4 cut(s) 11, 44, 288, 291
BlsI GCNGC 4 cut(s) 12, 45, 289, 292
Bme1390I CCNGG 2 cut(s) 24, 409
BmeT110I CYCGRG 1 cut(s) 14
BmiI GGNNCC 2 cut(s) 413, 449
BmrFI CCNGG 2 cut(s) 24, 409
BoxI GACNNNNGTC 1 cut(s) 25
Bpu10I CCTNAGC 1 cut(s) 47
BsaAI YACGTR 1 cut(s) 436
BsaI GGTCTC 2 cut(s) 13, 210
BsaJI CCNNGG 1 cut(s) 23
Bse1I ACTGG 1 cut(s) 288
BseBI CCWGG 2 cut(s) 24, 409
BseDI CCNNGG 1 cut(s) 23
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 1 cut(s) 288
BseXI GCAGC 3 cut(s) 30, 274, 277
BsgI GTGCAG 1 cut(s) 328
Bsh1285I CGRYCG 1 cut(s) 106
BsiEI CGRYCG 1 cut(s) 106
BsiHKCI CYCGRG 1 cut(s) 14
BsmAI GTCTC 3 cut(s) 13, 210, 324
Bso31I GGTCTC 2 cut(s) 13, 210
BsoBI CYCGRG 1 cut(s) 14
Bsp143I GATC 2 cut(s) 411, 447
BspACI CCGC 2 cut(s) 36, 106
BspCNI CTCAG 1 cut(s) 39
BspLI GGNNCC 2 cut(s) 413, 449
BspPI GGATC 4 cut(s) 406, 419, 442, 455
BspQI GCTCTTC 1 cut(s) 304
BspTNI GGTCTC 2 cut(s) 13, 210
BsrBI CCGCTC 1 cut(s) 36
BsrI ACTGG 1 cut(s) 288
BssECI CCNNGG 1 cut(s) 23
BssMI GATC 2 cut(s) 411, 447
Bst2UI CCWGG 2 cut(s) 24, 409
Bst6I CTCTTC 1 cut(s) 304
BstBAI YACGTR 1 cut(s) 436
BstC8I GCNNGC 3 cut(s) 180, 295, 384
BstDEI CTNAG 1 cut(s) 47
BstEII GGTNACC 1 cut(s) 94
BstKTI GATC 2 cut(s) 414, 450
BstMAI GTCTC 3 cut(s) 13, 210, 324
BstMBI GATC 2 cut(s) 411, 447
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstNI CCWGG 2 cut(s) 24, 409
BstNSI RCATGY 1 cut(s) 382
BstPAI GACNNNNGTC 1 cut(s) 25
BstPI GGTNACC 1 cut(s) 94
BstSCI CCNGG 2 cut(s) 22, 407
BstV1I GCAGC 3 cut(s) 30, 274, 277
BstX2I RGATCY 2 cut(s) 411, 447
BstYI RGATCY 2 cut(s) 411, 447
BtgZI GCGATG 1 cut(s) 67
BtsIMutI CAGTG 1 cut(s) 281
Cac8I GCNNGC 3 cut(s) 180, 295, 384
CviAII CATG 4 cut(s) 66, 91, 366, 379
CviJI RGCY 8 cut(s) 10, 171, 178, 244, 272, 287, 297, 375
CviKI_1 RGCY 8 cut(s) 10, 171, 178, 244, 272, 287, 297, 375
DdeI CTNAG 1 cut(s) 47
DpnI GATC 2 cut(s) 413, 449
DpnII GATC 2 cut(s) 411, 447
Eam1104I CTCTTC 1 cut(s) 304
EarI CTCTTC 1 cut(s) 304
Eco31I GGTCTC 2 cut(s) 13, 210
Eco72I CACGTG 1 cut(s) 436
Eco88I CYCGRG 1 cut(s) 14
Eco91I GGTNACC 1 cut(s) 94
EcoO65I GGTNACC 1 cut(s) 94
EcoRI GAATTC 1 cut(s) 116
EcoRII CCWGG 2 cut(s) 22, 407
FaeI CATG 4 cut(s) 69, 94, 369, 382
FaiI YATR 7 cut(s) 67, 92, 166, 198, 367, 380, 425
FatI CATG 4 cut(s) 65, 90, 365, 378
FauI CCCGC 1 cut(s) 29
Fnu4HI GCNGC 4 cut(s) 11, 44, 288, 291
Fsp4HI GCNGC 4 cut(s) 11, 44, 288, 291
FspBI CTAG 1 cut(s) 236
GluI GCNGC 4 cut(s) 11, 44, 288, 291
Hin1II CATG 4 cut(s) 69, 94, 369, 382
HphI GGTGA 4 cut(s) 88, 112, 269, 397
Hpy166II GTNNAC 1 cut(s) 362
Hpy188III TCNNGA 2 cut(s) 16, 188
Hpy8I GTNNAC 1 cut(s) 362
Hpy99I CGWCG 1 cut(s) 148
HpyAV CCTTC 2 cut(s) 230, 403
HpyCH4IV ACGT 1 cut(s) 435
HpyCH4V TGCA 4 cut(s) 293, 314, 345, 386
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpyF3I CTNAG 1 cut(s) 47
HpySE526I ACGT 1 cut(s) 435
Hsp92II CATG 4 cut(s) 69, 94, 369, 382
Kzo9I GATC 2 cut(s) 411, 447
LguI GCTCTTC 1 cut(s) 304
LmnI GCTCC 2 cut(s) 7, 168
LpnPI CCDG 9 cut(s) 9, 36, 57, 136, 269, 291, 333, 394, 421
Lsp1109I GCAGC 3 cut(s) 30, 274, 277
MaeI CTAG 1 cut(s) 236
MaeII ACGT 1 cut(s) 435
MaeIII GTNAC 3 cut(s) 94, 265, 403
MalI GATC 2 cut(s) 413, 449
MbiI CCGCTC 1 cut(s) 36
MboI GATC 2 cut(s) 411, 447
MboII GAAGA 1 cut(s) 291
MflI RGATCY 2 cut(s) 411, 447
MluCI AATT 4 cut(s) 59, 116, 230, 315
MnlI CCTC 3 cut(s) 42, 234, 439
MspR9I CCNGG 2 cut(s) 24, 409
MvaI CCWGG 2 cut(s) 24, 409
MwoI GCNNNNNNNGC 1 cut(s) 77
NdeII GATC 2 cut(s) 411, 447
NlaIII CATG 4 cut(s) 69, 94, 369, 382
NlaIV GGNNCC 2 cut(s) 413, 449
NmuCI GTSAC 2 cut(s) 94, 403
NspI RCATGY 1 cut(s) 382
PciSI GCTCTTC 1 cut(s) 304
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PkrI GCNGC 4 cut(s) 12, 45, 289, 292
PmaCI CACGTG 1 cut(s) 436
PmlI CACGTG 1 cut(s) 436
Ppu21I YACGTR 1 cut(s) 436
PshAI GACNNNNGTC 1 cut(s) 25
Psp6I CCWGG 2 cut(s) 22, 407
PspCI CACGTG 1 cut(s) 436
PspEI GGTNACC 1 cut(s) 94
PspGI CCWGG 2 cut(s) 22, 407
PspN4I GGNNCC 2 cut(s) 413, 449
PsuI RGATCY 2 cut(s) 411, 447
SapI GCTCTTC 1 cut(s) 304
SatI GCNGC 4 cut(s) 11, 44, 288, 291
Sau3AI GATC 2 cut(s) 411, 447
ScrFI CCNGG 2 cut(s) 24, 409
SpeI ACTAGT 1 cut(s) 235
Sse9I AATT 4 cut(s) 59, 116, 230, 315
SsiI CCGC 2 cut(s) 36, 106
SspMI CTAG 1 cut(s) 236
StyD4I CCNGG 2 cut(s) 22, 407
TaiI ACGT 1 cut(s) 438
TaqI TCGA 1 cut(s) 146
TasI AATT 4 cut(s) 59, 116, 230, 315
TscAI CASTG 1 cut(s) 288
TseFI GTSAC 2 cut(s) 94, 403
TseI GCWGC 4 cut(s) 10, 43, 287, 290
Tsp45I GTSAC 2 cut(s) 94, 403
TspDTI ATGAA 4 cut(s) 17, 54, 291, 326
TspRI CASTG 1 cut(s) 288
XapI RAATTY 1 cut(s) 116
XceI RCATGY 1 cut(s) 382
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XspI CTAG 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.