Rroxscaffold_6G00415130

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004667
Physical Location & Seq
Reverse (-)
37372881 .. 37376513
3633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00415130.1

Sequence Viewer

Length: 597 bp
ATGAAGGAGCTGATAGGTAGACCAGGGTCGGTTAGTGGGTTGCTCCTTAGAATCGGCCAATTTGGGTTTGCTGCTGCCGCCATCGGAGTCATGGTCTCCGCCGATCGCTTCGCCACATTCACCGCCTTCTGCTATTTGATTGCGTCAATGGGGCTACAAGTGCTATGGAGCTTTGGCCTTGCTTGTCTTGATATTTATGCTTTGAGGAGAAAGAGAGACCTCCAAAATCCAATCCTAGTTAGCCTATTTGTTGTCGGTGATTGGGTAACAGCTACTCTATCACTGGCTGCTGCAAGCGCTTCAGCAGGAATAGTAATTCTTTATGCAAAAGACACAAATTTCTGCACGTCACAATGGGATATACCATGCCGGAAGTTCCAGATTTCAGTGGCTTTTGCTTTCCTTACCTGGGCCTTCATTGCCCATTCATTTGTTTTCAATGTAAATGAAAAAATTTCTGTAACATGCCTCATCTTTTCTGGGGCAATTTACAGAATGACTGTAGAACTTAGAAGTGATATAATTGAGATACCGATATACAAGGGTGATCAATTGGGTGCAGAGAATGTAAATGGCTACTGTGGAAATGTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

198

Amino Acids

21.65

Weight (kDa)

7.56

Isoelectric Point (pI)

26.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 7 - 139 3e-29 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 19
AciI CCGC 3 cut(s) 78, 99, 123
AcoI YGGCCR 1 cut(s) 55
AcsI RAATTY 2 cut(s) 337, 453
AcuI CTGAAG 1 cut(s) 285
AfeI AGCGCT 1 cut(s) 298
AfiI CCNNNNNNNGG 1 cut(s) 409
AgsI TTSAA 2 cut(s) 439, 593
AjiI CACGTC 1 cut(s) 348
AjnI CCWGG 2 cut(s) 22, 407
AluBI AGCT 3 cut(s) 10, 171, 272
AluI AGCT 3 cut(s) 10, 171, 272
Alw26I GTCTC 2 cut(s) 100, 210
Aor51HI AGCGCT 1 cut(s) 298
AoxI GGCC 3 cut(s) 55, 175, 411
ApeKI GCWGC 4 cut(s) 71, 74, 287, 290
ApoI RAATTY 2 cut(s) 337, 453
AspLEI GCGC 1 cut(s) 299
AspS9I GGNCC 1 cut(s) 411
AsuHPI GGTGA 3 cut(s) 112, 269, 557
BbvI GCAGC 4 cut(s) 58, 61, 274, 277
BccI CCATC 1 cut(s) 89
BciT130I CCWGG 2 cut(s) 24, 409
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 2 cut(s) 100, 210
BfaI CTAG 1 cut(s) 236
BfmI CTRYAG 1 cut(s) 501
BfoI RGCGCY 1 cut(s) 300
BisI GCNGC 5 cut(s) 72, 75, 78, 288, 291
BlsI GCNGC 5 cut(s) 73, 76, 79, 289, 292
Bme1390I CCNGG 2 cut(s) 24, 409
BmgBI CACGTC 1 cut(s) 348
BmgT120I GGNCC 1 cut(s) 411
BmrFI CCNGG 2 cut(s) 24, 409
BoxI GACNNNNGTC 1 cut(s) 25
BsaI GGTCTC 2 cut(s) 100, 210
BsaJI CCNNGG 2 cut(s) 23, 408
BsaXI ACNNNNNCTCC 2 cut(s) 78, 108
Bsc4I CCNNNNNNNGG 1 cut(s) 409
Bse1I ACTGG 1 cut(s) 288
Bse3DI GCAATG 1 cut(s) 417
BseBI CCWGG 2 cut(s) 24, 409
BseDI CCNNGG 2 cut(s) 23, 408
BseLI CCNNNNNNNGG 1 cut(s) 409
BseMI GCAATG 1 cut(s) 417
BseNI ACTGG 1 cut(s) 288
BseRI GAGGAG 1 cut(s) 220
BseXI GCAGC 4 cut(s) 58, 61, 274, 277
BsgI GTGCAG 2 cut(s) 328, 579
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 3 cut(s) 57, 177, 413
BsiEI CGRYCG 1 cut(s) 106
BsiSI CCGG 1 cut(s) 370
BslI CCNNNNNNNGG 1 cut(s) 409
BsmAI GTCTC 2 cut(s) 100, 210
BsnI GGCC 3 cut(s) 57, 177, 413
Bso31I GGTCTC 2 cut(s) 100, 210
Bsp143I GATC 2 cut(s) 103, 547
BspACI CCGC 3 cut(s) 78, 99, 123
BspANI GGCC 3 cut(s) 57, 177, 413
BspTNI GGTCTC 2 cut(s) 100, 210
BsrDI GCAATG 1 cut(s) 417
BsrI ACTGG 1 cut(s) 288
BssECI CCNNGG 2 cut(s) 23, 408
BssMI GATC 2 cut(s) 103, 547
Bst2UI CCWGG 2 cut(s) 24, 409
Bst4CI ACNGT 2 cut(s) 502, 581
BstC8I GCNNGC 1 cut(s) 295
BstDEI CTNAG 2 cut(s) 47, 509
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 2 cut(s) 106, 550
BstMAI GTCTC 2 cut(s) 100, 210
BstMBI GATC 2 cut(s) 103, 547
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 4 cut(s) 77, 160, 296, 419
BstNI CCWGG 2 cut(s) 24, 409
BstNSI RCATGY 1 cut(s) 468
BstPAI GACNNNNGTC 1 cut(s) 25
BstSCI CCNGG 2 cut(s) 22, 407
BstSFI CTRYAG 1 cut(s) 501
BstV1I GCAGC 4 cut(s) 58, 61, 274, 277
BsuRI GGCC 3 cut(s) 57, 177, 413
BtrI CACGTC 1 cut(s) 348
BtsIMutI CAGTG 2 cut(s) 281, 393
Cac8I GCNNGC 1 cut(s) 295
CfoI GCGC 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 411
CseI GACGC 1 cut(s) 132
CviAII CATG 3 cut(s) 91, 366, 465
DdeI CTNAG 2 cut(s) 47, 509
DpnI GATC 2 cut(s) 105, 549
DpnII GATC 2 cut(s) 103, 547
EaeI YGGCCR 1 cut(s) 55
EciI GGCGGA 1 cut(s) 88
Eco31I GGTCTC 2 cut(s) 100, 210
Eco47III AGCGCT 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 285
EcoRII CCWGG 2 cut(s) 22, 407
FaeI CATG 3 cut(s) 94, 369, 468
FaiI YATR 9 cut(s) 92, 166, 198, 324, 362, 367, 466, 521, 538
FatI CATG 3 cut(s) 90, 365, 464
FbaI TGATCA 1 cut(s) 547
FblI GTMKAC 1 cut(s) 19
Fnu4HI GCNGC 5 cut(s) 72, 75, 78, 288, 291
Fsp4HI GCNGC 5 cut(s) 72, 75, 78, 288, 291
FspBI CTAG 1 cut(s) 236
GlaI GCGC 1 cut(s) 298
GluI GCNGC 5 cut(s) 72, 75, 78, 288, 291
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 3 cut(s) 57, 177, 413
HapII CCGG 1 cut(s) 370
HgaI GACGC 1 cut(s) 132
HhaI GCGC 1 cut(s) 299
Hin1II CATG 3 cut(s) 94, 369, 468
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HinfI GANTC 2 cut(s) 51, 87
HpaII CCGG 1 cut(s) 370
HphI GGTGA 3 cut(s) 112, 269, 557
Hpy166II GTNNAC 1 cut(s) 20
Hpy188I TCNGA 1 cut(s) 86
Hpy188III TCNNGA 2 cut(s) 188, 379
Hpy8I GTNNAC 1 cut(s) 20
HpyAV CCTTC 2 cut(s) 136, 424
HpyCH4III ACNGT 2 cut(s) 502, 581
HpyCH4IV ACGT 1 cut(s) 347
HpyCH4V TGCA 4 cut(s) 293, 326, 345, 560
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 160, 296, 419
HpyF3I CTNAG 2 cut(s) 47, 509
HpySE526I ACGT 1 cut(s) 347
Hsp92II CATG 3 cut(s) 94, 369, 468
HspAI GCGC 1 cut(s) 297
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 2 cut(s) 103, 547
LmnI GCTCC 3 cut(s) 7, 48, 168
LpnPI CCDG 9 cut(s) 9, 36, 269, 291, 383, 392, 394, 421, 465
Lsp1109I GCAGC 4 cut(s) 58, 61, 274, 277
MaeI CTAG 1 cut(s) 236
MaeII ACGT 1 cut(s) 347
MaeIII GTNAC 3 cut(s) 265, 348, 460
MalI GATC 2 cut(s) 105, 549
MboI GATC 2 cut(s) 103, 547
MfeI CAATTG 1 cut(s) 551
MluCI AATT 7 cut(s) 59, 315, 337, 453, 486, 522, 551
MlyI GAGTC 1 cut(s) 96
MnlI CCTC 3 cut(s) 198, 230, 479
MspI CCGG 1 cut(s) 370
MspR9I CCNGG 2 cut(s) 24, 409
MunI CAATTG 1 cut(s) 551
MvaI CCWGG 2 cut(s) 24, 409
MwoI GCNNNNNNNGC 4 cut(s) 77, 160, 296, 419
NdeII GATC 2 cut(s) 103, 547
NlaIII CATG 3 cut(s) 94, 369, 468
NmuCI GTSAC 1 cut(s) 348
NspI RCATGY 1 cut(s) 468
PfeI GAWTC 1 cut(s) 51
PkrI GCNGC 5 cut(s) 73, 76, 79, 289, 292
Ple19I CGATCG 1 cut(s) 106
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
PshAI GACNNNNGTC 1 cut(s) 25
Psp6I CCWGG 2 cut(s) 22, 407
PspGI CCWGG 2 cut(s) 22, 407
PspPI GGNCC 1 cut(s) 411
PvuI CGATCG 1 cut(s) 106
SatI GCNGC 5 cut(s) 72, 75, 78, 288, 291
Sau3AI GATC 2 cut(s) 103, 547
Sau96I GGNCC 1 cut(s) 411
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 2 cut(s) 24, 409
SetI ASST 7 cut(s) 12, 19, 173, 222, 274, 350, 410
SfcI CTRYAG 1 cut(s) 501
Sse9I AATT 7 cut(s) 59, 315, 337, 453, 486, 522, 551
SsiI CCGC 3 cut(s) 78, 99, 123
SspMI CTAG 1 cut(s) 236
StyD4I CCNGG 2 cut(s) 22, 407
TaaI ACNGT 2 cut(s) 502, 581
TaiI ACGT 1 cut(s) 350
TasI AATT 7 cut(s) 59, 315, 337, 453, 486, 522, 551
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 1 cut(s) 51
TscAI CASTG 2 cut(s) 288, 393
TseFI GTSAC 1 cut(s) 348
TseI GCWGC 4 cut(s) 71, 74, 287, 290
Tsp45I GTSAC 1 cut(s) 348
TspDTI ATGAA 4 cut(s) 17, 406, 417, 462
TspRI CASTG 2 cut(s) 288, 393
XapI RAATTY 2 cut(s) 337, 453
XceI RCATGY 1 cut(s) 468
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XmiI GTMKAC 1 cut(s) 19
XspI CTAG 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.