RLG00000027206

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004667
Physical Location & Seq
Forward (+)
9569961 .. 9572765
2805 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027206

Sequence Viewer

Length: 459 bp
ATGAAGGTGTTTTTTGGGAGTCCAGGAAATATAAGTGGGCTGCTCCTCAGAATTGGACAGTGCTTTTTTGCTGCTGCTTCTATTGGGGTCATGGTCTCTGCTCATGGCTTCTACACCTCTACTGCATTTTGCTATTTAATTGCATCAATGGGGCTTGAATTCCTTTGGAGTTTTGGACTTGCTTGCCTTGATTTTCATGCTTTGAGGTCAAAGAGGAGCTTGCAGAATCCTGTTTTAGTGAGCCTATTTGTTGTTGGAGACTGGGTGACTGCTACTCTTTCACTGGCAGGTGCGTGCTCATCAGCTGGGGTGGCAGTTCTGTTTTCAAGAGACTTGGGTTACTGCACCAAGACATTTCCATGTACCAGGTTCCAAATCGGGGTGGCTTTCGCTTTTATCTCATGGTTCCTCCTCGCTATTTCCTCACTTGTCATGTTTTGGCTGCTAGGTGCAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

153

Amino Acids

16.37

Weight (kDa)

8.88

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 7 - 137 2.1e-26 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 278
Acc36I ACCTGC 1 cut(s) 278
AcsI RAATTY 1 cut(s) 158
AfaI GTAC 1 cut(s) 364
AfiI CCNNNNNNNGG 1 cut(s) 379
AgsI TTSAA 2 cut(s) 158, 327
AjnI CCWGG 2 cut(s) 22, 365
AluBI AGCT 2 cut(s) 219, 305
AluI AGCT 2 cut(s) 219, 305
Alw21I GWGCWC 1 cut(s) 299
Alw26I GTCTC 3 cut(s) 100, 252, 324
ApeKI GCWGC 4 cut(s) 40, 71, 74, 442
ApoI RAATTY 1 cut(s) 158
AsuHPI GGTGA 1 cut(s) 277
Bbv12I GWGCWC 1 cut(s) 299
BbvI GCAGC 4 cut(s) 27, 58, 61, 429
BciT130I CCWGG 2 cut(s) 24, 367
BcoDI GTCTC 3 cut(s) 100, 252, 324
BfaI CTAG 1 cut(s) 446
BfuAI ACCTGC 1 cut(s) 278
BisI GCNGC 4 cut(s) 41, 72, 75, 443
BlsI GCNGC 4 cut(s) 42, 73, 76, 444
Bme1390I CCNGG 2 cut(s) 24, 367
BmiI GGNNCC 2 cut(s) 371, 407
BmrFI CCNGG 2 cut(s) 24, 367
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 1 cut(s) 152
BmuI ACTGGG 1 cut(s) 271
BsaI GGTCTC 1 cut(s) 100
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 1 cut(s) 379
Bse1I ACTGG 2 cut(s) 266, 288
BseBI CCWGG 2 cut(s) 24, 367
BseLI CCNNNNNNNGG 1 cut(s) 379
BseMII CTCAG 1 cut(s) 61
BseNI ACTGG 2 cut(s) 266, 288
BseRI GAGGAG 3 cut(s) 35, 229, 401
BseXI GCAGC 4 cut(s) 27, 58, 61, 429
BseYI CCCAGC 1 cut(s) 305
BsgI GTGCAG 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 299
BslI CCNNNNNNNGG 1 cut(s) 379
BsmAI GTCTC 3 cut(s) 100, 252, 324
Bso31I GGTCTC 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 299
BspCNI CTCAG 1 cut(s) 60
BspLI GGNNCC 2 cut(s) 371, 407
BspMI ACCTGC 1 cut(s) 278
BspTNI GGTCTC 1 cut(s) 100
BsrI ACTGG 2 cut(s) 266, 288
Bst2UI CCWGG 2 cut(s) 24, 367
Bst4CI ACNGT 1 cut(s) 60
BstC8I GCNNGC 3 cut(s) 184, 221, 295
BstDEI CTNAG 1 cut(s) 47
BstMAI GTCTC 3 cut(s) 100, 252, 324
BstMWI GCNNNNNNNGC 1 cut(s) 311
BstNI CCWGG 2 cut(s) 24, 367
BstSCI CCNGG 2 cut(s) 22, 365
BstV1I GCAGC 4 cut(s) 27, 58, 61, 429
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 3 cut(s) 65, 281, 459
BveI ACCTGC 1 cut(s) 278
Cac8I GCNNGC 3 cut(s) 184, 221, 295
CsiI ACCWGGT 1 cut(s) 365
Csp6I GTAC 1 cut(s) 363
CviAII CATG 6 cut(s) 91, 104, 197, 360, 402, 433
CviJI RGCY 8 cut(s) 40, 108, 154, 219, 243, 305, 386, 442
CviKI_1 RGCY 8 cut(s) 40, 108, 154, 219, 243, 305, 386, 442
CviQI GTAC 1 cut(s) 363
DdeI CTNAG 1 cut(s) 47
Eco31I GGTCTC 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 158
EcoRII CCWGG 2 cut(s) 22, 365
FaeI CATG 6 cut(s) 94, 107, 200, 363, 405, 436
FaiI YATR 7 cut(s) 32, 92, 105, 198, 361, 403, 434
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FatI CATG 6 cut(s) 90, 103, 196, 359, 401, 432
Fnu4HI GCNGC 4 cut(s) 41, 72, 75, 443
Fsp4HI GCNGC 4 cut(s) 41, 72, 75, 443
FspBI CTAG 1 cut(s) 446
GluI GCNGC 4 cut(s) 41, 72, 75, 443
GsaI CCCAGC 1 cut(s) 309
Hin1II CATG 6 cut(s) 94, 107, 200, 363, 405, 436
HinfI GANTC 2 cut(s) 19, 226
HphI GGTGA 1 cut(s) 277
Hpy188I TCNGA 1 cut(s) 50
Hpy188III TCNNGA 1 cut(s) 327
HpyCH4III ACNGT 1 cut(s) 60
HpyCH4V TGCA 5 cut(s) 125, 143, 223, 345, 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 311
HpyF3I CTNAG 1 cut(s) 47
Hsp92II CATG 6 cut(s) 94, 107, 200, 363, 405, 436
LmnI GCTCC 2 cut(s) 48, 216
LpnPI CCDG 9 cut(s) 9, 36, 243, 247, 269, 273, 291, 352, 379
Lsp1109I GCAGC 4 cut(s) 27, 58, 61, 429
LweI GCATC 1 cut(s) 152
MabI ACCWGGT 1 cut(s) 365
MaeI CTAG 1 cut(s) 446
MaeIII GTNAC 2 cut(s) 265, 338
MhlI GDGCHC 1 cut(s) 299
MluCI AATT 3 cut(s) 51, 138, 158
MlyI GAGTC 1 cut(s) 28
MmeI TCCRAC 1 cut(s) 235
MnlI CCTC 7 cut(s) 56, 127, 198, 207, 419, 422, 433
MseI TTAA 1 cut(s) 137
MslI CAYNNNNRTG 1 cut(s) 358
MspA1I CMGCKG 1 cut(s) 305
MspR9I CCNGG 2 cut(s) 24, 367
MvaI CCWGG 2 cut(s) 24, 367
MwoI GCNNNNNNNGC 1 cut(s) 311
NlaIII CATG 6 cut(s) 94, 107, 200, 363, 405, 436
NlaIV GGNNCC 2 cut(s) 371, 407
NmuCI GTSAC 1 cut(s) 265
PaqCI CACCTGC 1 cut(s) 278
PfeI GAWTC 1 cut(s) 226
PfoI TCCNGGA 1 cut(s) 22
PkrI GCNGC 4 cut(s) 42, 73, 76, 444
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
Psp6I CCWGG 2 cut(s) 22, 365
PspFI CCCAGC 1 cut(s) 305
PspGI CCWGG 2 cut(s) 22, 365
PspN4I GGNNCC 2 cut(s) 371, 407
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 1 cut(s) 364
RsaNI GTAC 1 cut(s) 363
RseI CAYNNNNRTG 1 cut(s) 358
SaqAI TTAA 1 cut(s) 137
SatI GCNGC 4 cut(s) 41, 72, 75, 443
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 2 cut(s) 24, 367
SduI GDGCHC 1 cut(s) 299
SetI ASST 8 cut(s) 9, 119, 209, 221, 292, 307, 371, 451
SexAI ACCWGGT 1 cut(s) 365
SfaNI GCATC 1 cut(s) 152
SmiMI CAYNNNNRTG 1 cut(s) 358
Sse9I AATT 3 cut(s) 51, 138, 158
SspMI CTAG 1 cut(s) 446
StyD4I CCNGG 2 cut(s) 22, 365
TaaI ACNGT 1 cut(s) 60
TasI AATT 3 cut(s) 51, 138, 158
TfiI GAWTC 1 cut(s) 226
Tru1I TTAA 1 cut(s) 137
Tru9I TTAA 1 cut(s) 137
TscAI CASTG 3 cut(s) 65, 288, 459
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 4 cut(s) 40, 71, 74, 442
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 2 cut(s) 17, 185
TspRI CASTG 3 cut(s) 65, 288, 459
XapI RAATTY 1 cut(s) 158
XspI CTAG 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.