Rorug01G0342600

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0004667
Physical Location & Seq
Reverse (-)
46086740 .. 46087966
1227 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0342600.1

Sequence Viewer

Length: 450 bp
ATGGATGCATTGGCAGCAGCAGCATTATCTTATGATTATGATGAAGGTCCTAAATTGGTTCATCGGCTTGACAGAGAGAGCAGTGGCCTCCTTTTAATGGGAAGAACAAAAGACAGTGTATCTCTTCTGCATTCGTTATACACTGACATCAACATTGGGAAATCATCATGCAGGGCTTGGAATGATGCATGTAGAACAAAATGTCAGAGGTACTGGGCATTGGTTATAGGGTCGCCCAAGGAAAAGGAAGGCTTAATTTGTGCTCCTCTCATGAAGGTGCTTCTTGACGATGGGAAGACAGAGAGGGTCATCTTGGCTCCTCAGTCAGGATTAGAAGCTTCCCAACCGGCTCTAACTCAATATCGGGTGCTTGGTCCTACGATAAATGGATGCTCATGGATTGAATTACAACCACTCACTAGCCGAAAGCATCAGGTACAAATTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

149

Amino Acids

16.47

Weight (kDa)

8.28

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 9 - 147 1.3e-14 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 441
AfaI GTAC 2 cut(s) 212, 438
AfiI CCNNNNNNNGG 2 cut(s) 97, 326
AgsI TTSAA 1 cut(s) 404
AluBI AGCT 1 cut(s) 338
AluI AGCT 1 cut(s) 338
Alw21I GWGCWC 1 cut(s) 265
AoxI GGCC 1 cut(s) 85
ApeKI GCWGC 3 cut(s) 14, 17, 20
ApoI RAATTY 1 cut(s) 441
AspS9I GGNCC 2 cut(s) 47, 374
AvaII GGWCC 2 cut(s) 47, 374
BbsI GAAGAC 1 cut(s) 302
Bbv12I GWGCWC 1 cut(s) 265
BbvI GCAGC 3 cut(s) 26, 29, 32
BccI CCATC 1 cut(s) 284
BfaI CTAG 1 cut(s) 420
BisI GCNGC 3 cut(s) 15, 18, 21
BlsI GCNGC 3 cut(s) 16, 19, 22
Bme18I GGWCC 2 cut(s) 47, 374
BmgT120I GGNCC 2 cut(s) 47, 374
BmiI GGNNCC 1 cut(s) 318
BmrI ACTGGG 1 cut(s) 223
BmsI GCATC 3 cut(s) 175, 380, 439
BmuI ACTGGG 1 cut(s) 223
BpiI GAAGAC 1 cut(s) 302
BsaJI CCNNGG 1 cut(s) 237
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 326
Bse118I RCCGGY 1 cut(s) 346
Bse1I ACTGG 1 cut(s) 218
BseDI CCNNGG 1 cut(s) 237
BseGI GGATG 2 cut(s) 10, 395
BseLI CCNNNNNNNGG 2 cut(s) 97, 326
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 1 cut(s) 218
BseRI GAGGAG 2 cut(s) 255, 309
BseXI GCAGC 3 cut(s) 26, 29, 32
BshFI GGCC 1 cut(s) 87
BsiHKAI GWGCWC 1 cut(s) 265
BsiSI CCGG 1 cut(s) 347
BslI CCNNNNNNNGG 2 cut(s) 97, 326
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 1 cut(s) 87
Bsp1286I GDGCHC 1 cut(s) 265
BspANI GGCC 1 cut(s) 87
BspCNI CTCAG 1 cut(s) 334
BspHI TCATGA 1 cut(s) 270
BspLI GGNNCC 1 cut(s) 318
BsrFI RCCGGY 1 cut(s) 346
BsrI ACTGG 1 cut(s) 218
BssAI RCCGGY 1 cut(s) 346
BssECI CCNNGG 1 cut(s) 237
BssT1I CCWWGG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 116
Bst6I CTCTTC 1 cut(s) 129
BstDEI CTNAG 1 cut(s) 321
BstENI CCTNNNNNAGG 1 cut(s) 324
BstF5I GGATG 2 cut(s) 10, 395
BstMWI GCNNNNNNNGC 2 cut(s) 14, 20
BstNSI RCATGY 1 cut(s) 192
BstV1I GCAGC 3 cut(s) 26, 29, 32
BstV2I GAAGAC 1 cut(s) 302
BsuRI GGCC 1 cut(s) 87
BtsCI GGATG 2 cut(s) 10, 395
BtsI GCAGTG 1 cut(s) 88
BtsIMutI CAGTG 3 cut(s) 88, 121, 141
CciI TCATGA 1 cut(s) 270
Cfr10I RCCGGY 1 cut(s) 346
Cfr13I GGNCC 2 cut(s) 47, 374
Csp6I GTAC 2 cut(s) 211, 437
CviAII CATG 4 cut(s) 168, 189, 271, 396
CviJI RGCY 8 cut(s) 67, 87, 176, 252, 317, 338, 350, 423
CviKI_1 RGCY 8 cut(s) 67, 87, 176, 252, 317, 338, 350, 423
CviQI GTAC 2 cut(s) 211, 437
DdeI CTNAG 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 237
Eco47I GGWCC 2 cut(s) 47, 374
EcoNI CCTNNNNNAGG 1 cut(s) 324
EcoO109I RGGNCCY 1 cut(s) 47
EcoT14I CCWWGG 1 cut(s) 237
EcoT22I ATGCAT 2 cut(s) 10, 190
ErhI CCWWGG 1 cut(s) 237
FaeI CATG 4 cut(s) 171, 192, 274, 399
FaiI YATR 8 cut(s) 33, 39, 139, 169, 190, 227, 272, 397
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FatI CATG 4 cut(s) 167, 188, 270, 395
Fnu4HI GCNGC 3 cut(s) 15, 18, 21
FokI GGATG 2 cut(s) 17, 402
Fsp4HI GCNGC 3 cut(s) 15, 18, 21
FspBI CTAG 1 cut(s) 420
GluI GCNGC 3 cut(s) 15, 18, 21
HaeIII GGCC 1 cut(s) 87
HapII CCGG 1 cut(s) 347
Hin1II CATG 4 cut(s) 171, 192, 274, 399
HindIII AAGCTT 1 cut(s) 336
HpaII CCGG 1 cut(s) 347
Hpy188I TCNGA 1 cut(s) 207
Hpy188III TCNNGA 3 cut(s) 271, 284, 327
HpyAV CCTTC 3 cut(s) 38, 242, 268
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4V TGCA 4 cut(s) 8, 130, 171, 188
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 20
HpyF3I CTNAG 1 cut(s) 321
Hsp92II CATG 4 cut(s) 171, 192, 274, 399
LmnI GCTCC 2 cut(s) 268, 322
LpnPI CCDG 5 cut(s) 157, 199, 312, 360, 419
Lsp1109I GCAGC 3 cut(s) 26, 29, 32
LweI GCATC 3 cut(s) 175, 380, 439
MaeI CTAG 1 cut(s) 420
MboII GAAGA 3 cut(s) 114, 116, 307
MhlI GDGCHC 1 cut(s) 265
MluCI AATT 4 cut(s) 53, 255, 404, 441
MnlI CCTC 5 cut(s) 98, 201, 276, 297, 330
Mph1103I ATGCAT 2 cut(s) 10, 190
MseI TTAA 3 cut(s) 95, 254, 448
MslI CAYNNNNRTG 1 cut(s) 275
MspI CCGG 1 cut(s) 347
Mva1269I GAATGC 1 cut(s) 130
MwoI GCNNNNNNNGC 2 cut(s) 14, 20
NlaIII CATG 4 cut(s) 171, 192, 274, 399
NlaIV GGNNCC 1 cut(s) 318
NsiI ATGCAT 2 cut(s) 10, 190
NspI RCATGY 1 cut(s) 192
PagI TCATGA 1 cut(s) 270
PctI GAATGC 1 cut(s) 130
PkrI GCNGC 3 cut(s) 16, 19, 22
PpuMI RGGWCCY 1 cut(s) 47
Psp5II RGGWCCY 1 cut(s) 47
PspN4I GGNNCC 1 cut(s) 318
PspPI GGNCC 2 cut(s) 47, 374
PspPPI RGGWCCY 1 cut(s) 47
RsaI GTAC 2 cut(s) 212, 438
RsaNI GTAC 2 cut(s) 211, 437
RseI CAYNNNNRTG 1 cut(s) 275
SaqAI TTAA 3 cut(s) 95, 254, 448
SatI GCNGC 3 cut(s) 15, 18, 21
Sau96I GGNCC 2 cut(s) 47, 374
SduI GDGCHC 1 cut(s) 265
SetI ASST 5 cut(s) 49, 212, 279, 340, 438
SfaNI GCATC 3 cut(s) 175, 380, 439
SinI GGWCC 2 cut(s) 47, 374
SmiMI CAYNNNNRTG 1 cut(s) 275
Sse9I AATT 4 cut(s) 53, 255, 404, 441
SspMI CTAG 1 cut(s) 420
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 1 cut(s) 116
TasI AATT 4 cut(s) 53, 255, 404, 441
Tru1I TTAA 3 cut(s) 95, 254, 448
Tru9I TTAA 3 cut(s) 95, 254, 448
TscAI CASTG 3 cut(s) 88, 121, 148
TseI GCWGC 3 cut(s) 14, 17, 20
TspDTI ATGAA 3 cut(s) 50, 57, 287
TspRI CASTG 3 cut(s) 88, 121, 148
VpaK11BI GGWCC 2 cut(s) 47, 374
XagI CCTNNNNNAGG 1 cut(s) 324
XapI RAATTY 1 cut(s) 441
XceI RCATGY 1 cut(s) 192
XspI CTAG 1 cut(s) 420
Zsp2I ATGCAT 2 cut(s) 10, 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.