AT5G04070

short-chain dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003848
Physical Location & Seq
Forward (+)
1102071 .. 1104446
2376 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04070.1

Sequence Viewer

Length: 1080 bp
ATGGAGAATTTGAAGGAGGCTTTGCGTTTTATCTGCTCATCTAATTTCTGGAGGATGGTTCTGTTTTGGAACATTGCCCTCCTTTTTTCGTACTTTCAATTGCTTAAAAAGAGTATCTTTGCCCCCAAATCAAGTTCCTCTTCTTCTTGCTCCAAATTCAATCATTCACACACACCTGTTTGTGTTATAACTGGTGCTACTTCGGGACTTGGTAAGGCCACTGCGTTTGCTCTTTCAAGGAAAGGTTTCTACGTTGTTCTTGTTGGACGGTCCTCGCACTTACTATCAAAGACCTTGAGTGATATTAAGAGGCAGAACGAGGATGCCAAACTCAAAGCTTTTGAAGTTGACATGTCGTCTTTTCAATTAGTTTTAAAGTTCAGAAGCTCTCTAGAGCAATGGCTTTTTGAGTCAGATTTGCATTCTTCAGTCCAGCTTTTGGTGAACAATGCTGGAATACTGGCAACATCTAGTCGTCCAACTGTTGAAGGCTTTGACAGGATGATTGCTACAAATTATGTCGGCGCTTTCTCTTTGACCAAACTTCTCTTACCGCTCCTGAGAAACAGCCCTGTGCCTTCAAGAGTAGTGAATGTTACATCTTTTACACATCGTTCTGCTTTTACTGGGAGGTTTGACATGGATTCTGTTACTGGAGTGAACTTTTCAAGATCAAAGCAATATCCTTGTGCTAGGATCTATGAGTATTCTAAATTATGCCTTCTACTTTTCTCGTACGAGCTGCATAGACAGCTTCACCTCATGGATGATTCACATCACATCTCTGTTGTAGCGGTAGATCCAGGAGCAGTAAAAACAAACATAATGCATGAGCTTCCTTCATATATACAAGTCATTGCGTTTTGTGGTCTCAAGATTCTTGGACTGATGCAGTCTCCAGAGGATGCAGCTGAATCTGTCATTGATGCTGCTTTAGCTCCACCTGAAATATCAGGCAAATATTTCTTTGGCGGGAGGACCATTGAATCTTCAACACTTTCCAGTGACCCGAAAATGGCTAAGGAACTTTGGGACACATCATGTCTCATATTTAACGAATTGCAGCAAACTCACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0008150 GO:0009893 GO:0009894 GO:0009896 GO:0010506 GO:0010508 GO:0019222 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

359

Amino Acids

40.07

Weight (kDa)

8.87

Isoelectric Point (pI)

50.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 60 - 204 1.5e-21 short chain dehydrogenase
adh_short_C2 PF13561 65 - 210 3.7e-11 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 188
AccB7I CCANNNNNTGG 1 cut(s) 439
AccBSI CCGCTC 1 cut(s) 556
AciI CCGC 3 cut(s) 554, 794, 972
AclWI GGATC 2 cut(s) 704, 794
AcsI RAATTY 2 cut(s) 7, 155
AcuI CTGAAG 1 cut(s) 411
AfaI GTAC 2 cut(s) 92, 737
AfiI CCNNNNNNNGG 2 cut(s) 439, 1015
AflIII ACRYGT 1 cut(s) 351
AhdI GACNNNNNGTC 1 cut(s) 355
AjnI CCWGG 1 cut(s) 802
AluBI AGCT 8 cut(s) 338, 387, 436, 742, 754, 835, 911, 938
AluI AGCT 8 cut(s) 338, 387, 436, 742, 754, 835, 911, 938
Alw26I GTCTC 3 cut(s) 875, 900, 1049
AlwI GGATC 2 cut(s) 704, 794
AoxI GGCC 1 cut(s) 216
ApeKI GCWGC 4 cut(s) 742, 908, 929, 1063
ApoI RAATTY 2 cut(s) 7, 155
Asp700I GAANNNNTTC 1 cut(s) 245
AspLEI GCGC 1 cut(s) 527
AspS9I GGNCC 2 cut(s) 270, 978
AsuHPI GGTGA 2 cut(s) 454, 749
AvaII GGWCC 2 cut(s) 270, 978
BbvI GCAGC 4 cut(s) 729, 916, 920, 1075
BccI CCATC 1 cut(s) 49
BciT130I CCWGG 1 cut(s) 804
BcoDI GTCTC 3 cut(s) 875, 900, 1049
BfaI CTAG 3 cut(s) 392, 471, 693
BfoI RGCGCY 1 cut(s) 528
BisI GCNGC 4 cut(s) 743, 909, 930, 1064
BlsI GCNGC 4 cut(s) 744, 910, 931, 1065
Bme1390I CCNGG 1 cut(s) 804
Bme18I GGWCC 2 cut(s) 270, 978
BmeRI GACNNNNNGTC 1 cut(s) 355
BmgT120I GGNCC 2 cut(s) 270, 978
BmrFI CCNGG 1 cut(s) 804
BmrI ACTGGG 1 cut(s) 636
BmsI GCATC 4 cut(s) 313, 879, 895, 916
BmuI ACTGGG 1 cut(s) 636
BpmI CTGGAG 3 cut(s) 70, 675, 882
Bpu10I CCTNAGC 1 cut(s) 1020
BpuEI CTTGAG 2 cut(s) 316, 857
BsaBI GATNNNNATC 1 cut(s) 774
BsaI GGTCTC 1 cut(s) 875
Bsc4I CCNNNNNNNGG 2 cut(s) 439, 1015
Bse1I ACTGG 5 cut(s) 196, 465, 631, 658, 1002
Bse3DI GCAATG 3 cut(s) 72, 404, 855
Bse8I GATNNNNATC 1 cut(s) 774
BseBI CCWGG 1 cut(s) 804
BseGI GGATG 5 cut(s) 60, 328, 507, 772, 910
BseJI GATNNNNATC 1 cut(s) 774
BseLI CCNNNNNNNGG 2 cut(s) 439, 1015
BseMI GCAATG 3 cut(s) 72, 404, 855
BseMII CTCAG 1 cut(s) 551
BseNI ACTGG 5 cut(s) 196, 465, 631, 658, 1002
BseXI GCAGC 4 cut(s) 729, 916, 920, 1075
BshFI GGCC 1 cut(s) 218
BsiWI CGTACG 1 cut(s) 735
BslFI GGGAC 2 cut(s) 219, 1046
BslI CCNNNNNNNGG 2 cut(s) 439, 1015
BsmAI GTCTC 3 cut(s) 875, 900, 1049
BsmFI GGGAC 2 cut(s) 219, 1046
BsmI GAATGC 1 cut(s) 421
BsnI GGCC 1 cut(s) 218
Bso31I GGTCTC 1 cut(s) 875
Bsp143I GATC 3 cut(s) 671, 696, 799
BspACI CCGC 3 cut(s) 554, 794, 972
BspANI GGCC 1 cut(s) 218
BspCNI CTCAG 1 cut(s) 552
BspPI GGATC 2 cut(s) 704, 794
BspTNI GGTCTC 1 cut(s) 875
BsrBI CCGCTC 1 cut(s) 556
BsrDI GCAATG 3 cut(s) 72, 404, 855
BsrI ACTGG 5 cut(s) 196, 465, 631, 658, 1002
BssMI GATC 3 cut(s) 671, 696, 799
Bst2UI CCWGG 1 cut(s) 804
Bst4CI ACNGT 2 cut(s) 270, 484
Bst6I CTCTTC 1 cut(s) 145
BstDEI CTNAG 2 cut(s) 560, 1020
BstF5I GGATG 5 cut(s) 60, 328, 507, 772, 910
BstH2I RGCGCY 1 cut(s) 528
BstHHI GCGC 1 cut(s) 527
BstKTI GATC 3 cut(s) 674, 699, 802
BstMAI GTCTC 3 cut(s) 875, 900, 1049
BstMBI GATC 3 cut(s) 671, 696, 799
BstMWI GCNNNNNNNGC 2 cut(s) 751, 935
BstNI CCWGG 1 cut(s) 804
BstNSI RCATGY 1 cut(s) 355
BstSCI CCNGG 1 cut(s) 802
BstV1I GCAGC 4 cut(s) 729, 916, 920, 1075
BstX2I RGATCY 2 cut(s) 696, 799
BstYI RGATCY 2 cut(s) 696, 799
BsuRI GGCC 1 cut(s) 218
BtsCI GGATG 5 cut(s) 60, 328, 507, 772, 910
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 2 cut(s) 219, 1009
CfoI GCGC 1 cut(s) 527
Cfr13I GGNCC 2 cut(s) 270, 978
Csp6I GTAC 2 cut(s) 91, 736
CspCI CAANNNNNGTGG 2 cut(s) 208, 243
CviAII CATG 5 cut(s) 352, 640, 763, 830, 1041
CviQI GTAC 2 cut(s) 91, 736
DdeI CTNAG 2 cut(s) 560, 1020
DpnI GATC 3 cut(s) 673, 698, 801
DpnII GATC 3 cut(s) 671, 696, 799
DraI TTTAAA 1 cut(s) 375
DriI GACNNNNNGTC 1 cut(s) 355
Eam1104I CTCTTC 1 cut(s) 145
Eam1105I GACNNNNNGTC 1 cut(s) 355
EarI CTCTTC 1 cut(s) 145
Eco31I GGTCTC 1 cut(s) 875
Eco47I GGWCC 2 cut(s) 270, 978
Eco57I CTGAAG 1 cut(s) 411
EcoRII CCWGG 1 cut(s) 802
EcoT22I ATGCAT 1 cut(s) 831
FaeI CATG 5 cut(s) 355, 643, 766, 833, 1044
FalI AAGNNNNNCTT 4 cut(s) 101, 133, 124, 156
FaqI GGGAC 2 cut(s) 219, 1046
FatI CATG 5 cut(s) 351, 639, 762, 829, 1040
FauI CCCGC 1 cut(s) 965
Fnu4HI GCNGC 4 cut(s) 743, 909, 930, 1064
FokI GGATG 5 cut(s) 67, 335, 514, 779, 917
Fsp4HI GCNGC 4 cut(s) 743, 909, 930, 1064
FspBI CTAG 3 cut(s) 392, 471, 693
GlaI GCGC 1 cut(s) 526
GluI GCNGC 4 cut(s) 743, 909, 930, 1064
GsuI CTGGAG 3 cut(s) 70, 675, 882
HaeII RGCGCY 1 cut(s) 528
HaeIII GGCC 1 cut(s) 218
HhaI GCGC 1 cut(s) 527
Hin1II CATG 5 cut(s) 355, 643, 766, 833, 1044
Hin6I GCGC 1 cut(s) 525
HinP1I GCGC 1 cut(s) 525
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 336
HinfI GANTC 6 cut(s) 410, 644, 770, 877, 914, 986
HphI GGTGA 2 cut(s) 454, 749
Hpy166II GTNNAC 3 cut(s) 349, 445, 661
Hpy188I TCNGA 2 cut(s) 383, 415
Hpy188III TCNNGA 8 cut(s) 49, 204, 392, 559, 582, 669, 874, 899
Hpy8I GTNNAC 3 cut(s) 349, 445, 661
HpyAV CCTTC 5 cut(s) 7, 482, 588, 731, 849
HpyCH4III ACNGT 2 cut(s) 270, 484
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 6 cut(s) 421, 745, 829, 892, 908, 1063
HpyF10VI GCNNNNNNNGC 2 cut(s) 751, 935
HpyF3I CTNAG 2 cut(s) 560, 1020
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 5 cut(s) 355, 643, 766, 833, 1044
HspAI GCGC 1 cut(s) 525
Kzo9I GATC 3 cut(s) 671, 696, 799
LmnI GCTCC 4 cut(s) 155, 561, 806, 943
Lsp1109I GCAGC 4 cut(s) 729, 916, 920, 1075
LweI GCATC 4 cut(s) 313, 879, 895, 916
MaeI CTAG 3 cut(s) 392, 471, 693
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 3 cut(s) 595, 649, 1004
MalI GATC 3 cut(s) 673, 698, 801
MbiI CCGCTC 1 cut(s) 556
MboI GATC 3 cut(s) 671, 696, 799
MboII GAAGA 4 cut(s) 132, 135, 417, 981
MfeI CAATTG 1 cut(s) 98
MflI RGATCY 2 cut(s) 696, 799
MluCI AATT 8 cut(s) 7, 43, 98, 155, 365, 514, 713, 1058
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 2 cut(s) 244, 503
Mph1103I ATGCAT 1 cut(s) 831
MroXI GAANNNNTTC 1 cut(s) 245
MseI TTAA 4 cut(s) 105, 306, 374, 1053
MspA1I CMGCKG 1 cut(s) 911
MspR9I CCNGG 1 cut(s) 804
MunI CAATTG 1 cut(s) 98
Mva1269I GAATGC 1 cut(s) 421
MvaI CCWGG 1 cut(s) 804
MwoI GCNNNNNNNGC 2 cut(s) 751, 935
NdeII GATC 3 cut(s) 671, 696, 799
NlaIII CATG 5 cut(s) 355, 643, 766, 833, 1044
NmuCI GTSAC 1 cut(s) 1004
NsiI ATGCAT 1 cut(s) 831
NspI RCATGY 1 cut(s) 355
PciI ACATGT 1 cut(s) 351
PctI GAATGC 1 cut(s) 421
PdmI GAANNNNTTC 1 cut(s) 245
PfeI GAWTC 5 cut(s) 644, 770, 877, 914, 986
Pfl23II CGTACG 1 cut(s) 735
PflMI CCANNNNNTGG 1 cut(s) 439
PfoI TCCNGGA 1 cut(s) 802
PkrI GCNGC 4 cut(s) 744, 910, 931, 1065
PleI GAGTC 1 cut(s) 418
PpsI GAGTC 1 cut(s) 418
PscI ACATGT 1 cut(s) 351
PsiI TTATAA 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 802
PspGI CCWGG 1 cut(s) 802
PspLI CGTACG 1 cut(s) 735
PspPI GGNCC 2 cut(s) 270, 978
PsuI RGATCY 2 cut(s) 696, 799
PvuII CAGCTG 1 cut(s) 911
RsaI GTAC 2 cut(s) 92, 737
RsaNI GTAC 2 cut(s) 91, 736
SaqAI TTAA 4 cut(s) 105, 306, 374, 1053
SatI GCNGC 4 cut(s) 743, 909, 930, 1064
Sau3AI GATC 3 cut(s) 671, 696, 799
Sau96I GGNCC 2 cut(s) 270, 978
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 1 cut(s) 804
SfaNI GCATC 4 cut(s) 313, 879, 895, 916
SinI GGWCC 2 cut(s) 270, 978
SmlI CTYRAG 2 cut(s) 295, 872
SmoI CTYRAG 2 cut(s) 295, 872
Sse9I AATT 8 cut(s) 7, 43, 98, 155, 365, 514, 713, 1058
SsiI CCGC 3 cut(s) 554, 794, 972
SspI AATATT 1 cut(s) 962
SspMI CTAG 3 cut(s) 392, 471, 693
StyD4I CCNGG 1 cut(s) 802
TaaI ACNGT 2 cut(s) 270, 484
TaiI ACGT 1 cut(s) 255
TasI AATT 8 cut(s) 7, 43, 98, 155, 365, 514, 713, 1058
TfiI GAWTC 5 cut(s) 644, 770, 877, 914, 986
Tru1I TTAA 4 cut(s) 105, 306, 374, 1053
Tru9I TTAA 4 cut(s) 105, 306, 374, 1053
TscAI CASTG 2 cut(s) 226, 1009
TseFI GTSAC 1 cut(s) 1004
TseI GCWGC 4 cut(s) 742, 908, 929, 1063
Tsp45I GTSAC 1 cut(s) 1004
TspDTI ATGAA 1 cut(s) 831
TspRI CASTG 2 cut(s) 226, 1009
Van91I CCANNNNNTGG 1 cut(s) 439
VpaK11BI GGWCC 2 cut(s) 270, 978
XapI RAATTY 2 cut(s) 7, 155
XbaI TCTAGA 1 cut(s) 391
XceI RCATGY 1 cut(s) 355
XmnI GAANNNNTTC 1 cut(s) 245
XspI CTAG 3 cut(s) 392, 471, 693
Zsp2I ATGCAT 1 cut(s) 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.