Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004391

Physical Location & Seq

Genomic Coordinates Reverse (-)

2663499 .. 2665826

Gene Structure 2328 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G08280.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1149 bp

ATGGATATTGCTTCGTCATCTCTCTCACAAGCTCACAAAGTCGTTCTCACGCGTCAACCTTCTTCCCGGGTCAACACCTGCTCCCTTGGCTCGGTCTCCGCTATCGGATTCTCTCTTCCGCAGATTAGCTCTCCAGCTTTAGGTAAATGTCGCCGGAAACAAAGCTCTTCTGGGTTCGTGAAAGCTTGTGTTGCTGTTGAACAGAAAACCCGAACTGCTATCATCAGAATTGGCACAAGGGGAAGTCCTCTAGCACTTGCTCAAGCATACGAGACGCGAGAAAAGCTCAAGAAGAAACACCCTGAACTCGTTGAAGATGGAGCTATTCATATCGAGATCATTAAAACGACTGGTGATAAGATTCTTTCGCAACCGCTTGCTGATATTGGTGGGAAAGGACTTTTCACCAAAGAAATAGACGAGGCCTTGATAAATGGTCATATTGACATAGCTGTGCACTCAATGAAAGATGTCCCAACTTACTTACCAGAAAAAACGATTTTACCTTGTAACCTTCCGCGTGAGGATGTTCGAGATGCGTTTATTTGTCTAACTGCAGCCACGTTAGCTGAGCTTCCAGCTGGAAGCGTTGTGGGAACAGCTTCTCTCAGGAGAAAATCGCAGATTCTCCACAAATATCCTGCATTACATGTTGAGGAAAACTTCAGGGGTAATGTGCAGACAAGACTATCAAAACTACAAGGAGGAAAGGTCCAAGCAACTCTATTAGCACTAGCTGGTCTTAAGAGATTGAGTATGACAGAGAATGTCGCATCTATCTTATCTCTCGATGAAATGCTTCCAGCTGTTGCTCAAGGAGCTATTGGAATTGCCTGTAGAACTGATGATGATAAAATGGCAACTTACTTAGCCTCACTGAACCACGAGGAAACAAGACTAGCGATTTCATGCGAGAGAGCTTTTCTTGAAACGCTAGATGGCTCATGCCGTACTCCTATTGCTGGATACGCATCCAAGGACGAAGAAGGCAACTGCATTTTCAGAGGATTGGTTGCTTCCCCTGACGGTACTAAAGTTCTTGAGACCTCAAGAAAAGGTCCATACGTGTATGAAGACATGGTGAAGATGGGAAAAGACGCGGGGCAAGAATTGCTATCTCGTGCTGGTCCTGGCTTCTTCGGCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004418 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006778 GO:0006779 GO:0006783 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009451 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009941 GO:0009987 GO:0015994 GO:0015995 GO:0016070 GO:0016740 GO:0016765 GO:0018130 GO:0019438 GO:0031967 GO:0031975 GO:0033013 GO:0033014 GO:0034641 GO:0042168 GO:0042440 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046148 GO:0046483 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0051704 GO:0051707 GO:0071704 GO:0090304 GO:0098542 GO:1900865 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120

Pfam Domains

Protein Families

Porphobil_deam Porphobil_deamC

Protein Analysis

382

Amino Acids

41.04

Weight (kDa)

8.75

Isoelectric Point (pI)

46.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Porphobil_deam	PF01379	75 - 287	2.1e-78	Porphobilinogen deaminase, dipyromethane cofactor binding domain
Porphobil_deamC	PF03900	301 - 372	7.7e-14	Porphobilinogen deaminase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004391)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08280
fragaria_vesca	FvH4_4g37020 FvH4_7g04700
malus_domestica	MD13G1004100.v1.1 MD16G1003400.v1.1
prunus_persica	Prupe.1G348400_v2.0.a1 Prupe.1G348400_v2.0.a1 Prupe.1G348400_v2.0.a1
pyrus_communis	pycom13g00340 pycom16g00240
rosa_chinensis	RchiOBHm_Chr1g0330101 RchiOBHm_Chr4g0446801
rosa_laevigata	RLG00000005629 RLG00000029832
rosa_multiflora	Rmu_co8512413.1_g000001 Rmu_sc0001753.1_g000022 Rmu_sc0004673.1_g000003
rosa_roxburghii	Rroxscaffold_4G00320380 Rroxscaffold_5G00387180
rosa_rugosa	Rorug01G0085800 Rorug01G0085900 Rorug04G0374900
rosa_samantha	Rh1AG104400 Rh1CG100000 Rh1DG107300 Rh1DG108400 Rh4AG436200 Rh4BG445300 Rh4CG462600 Rh4DG444000
rosa_wichuraiana	Rw0G015900 Rw4G037310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	86
Acc36I	ACCTGC	1 cut(s)	86
AccII	CGCG	4 cut(s)	52, 277, 520, 1100
AciI	CCGC	5 cut(s)	99, 119, 374, 518, 1100
AcuI	CTGAAG	1 cut(s)	649
AfaI	GTAC	2 cut(s)	952, 1030
AfiI	CCNNNNNNNGG	3 cut(s)	91, 140, 962
AflII	CTTAAG	1 cut(s)	743
AflIII	ACRYGT	3 cut(s)	50, 649, 1065
AgsI	TTSAA	3 cut(s)	200, 314, 929
AjnI	CCWGG	1 cut(s)	1129
Alw21I	GWGCWC	1 cut(s)	459
Alw26I	GTCTC	3 cut(s)	100, 266, 1037
Alw44I	GTGCAC	1 cut(s)	455
Ama87I	CYCGRG	1 cut(s)	66
AoxI	GGCC	1 cut(s)	423
ApaLI	GTGCAC	1 cut(s)	455
ApeKI	GCWGC	1 cut(s)	557
Asp700I	GAANNNNTTC	2 cut(s)	601, 798
AspS9I	GGNCC	3 cut(s)	712, 1058, 1127
AsuC2I	CCSGG	2 cut(s)	67, 68
AsuHPI	GGTGA	3 cut(s)	365, 397, 1093
AvaI	CYCGRG	1 cut(s)	66
AvaII	GGWCC	3 cut(s)	712, 1058, 1127
BaeGI	GKGCMC	1 cut(s)	459
BaeI	ACNNNNGTAYC	2 cut(s)	1020, 1053
BauI	CACGAG	2 cut(s)	884, 1119
BbsI	GAAGAC	1 cut(s)	1080
Bbv12I	GWGCWC	1 cut(s)	459
BbvI	GCAGC	1 cut(s)	569
BccI	CCATC	3 cut(s)	311, 932, 1081
BceAI	ACGGC	1 cut(s)	933
BciT130I	CCWGG	1 cut(s)	1131
BciVI	GTATCC	1 cut(s)	959
BcnI	CCSGG	2 cut(s)	67, 68
BcoDI	GTCTC	3 cut(s)	100, 266, 1037
BfaI	CTAG	4 cut(s)	251, 734, 899, 935
BfmI	CTRYAG	2 cut(s)	555, 835
BfrI	CTTAAG	1 cut(s)	743
BfuAI	ACCTGC	1 cut(s)	86
BfuI	GTATCC	1 cut(s)	959
BisI	GCNGC	1 cut(s)	558
BlpI	GCTNAGC	1 cut(s)	570
BlsI	GCNGC	1 cut(s)	559
Bme1390I	CCNGG	3 cut(s)	67, 68, 1131
Bme18I	GGWCC	3 cut(s)	712, 1058, 1127
BmeT110I	CYCGRG	1 cut(s)	66
BmgT120I	GGNCC	3 cut(s)	712, 1058, 1127
BmrFI	CCNGG	3 cut(s)	67, 68, 1131
BmsI	GCATC	3 cut(s)	526, 782, 980
BpiI	GAAGAC	1 cut(s)	1080
BplI	GAGNNNNNCTC	2 cut(s)	270, 302
BpmI	CTGGAG	1 cut(s)	117
Bpu1102I	GCTNAGC	1 cut(s)	570
BpuEI	CTTGAG	5 cut(s)	246, 272, 798, 1033, 1061
BpuMI	CCSGG	2 cut(s)	67, 68
BsaAI	YACGTR	1 cut(s)	1066
BsaBI	GATNNNNATC	1 cut(s)	970
BsaI	GGTCTC	2 cut(s)	100, 1037
BsaJI	CCNNGG	3 cut(s)	66, 85, 975
BsaXI	ACNNNNNCTCC	4 cut(s)	65, 95, 696, 726
Bsc4I	CCNNNNNNNGG	3 cut(s)	91, 140, 962
Bse1I	ACTGG	1 cut(s)	355
Bse8I	GATNNNNATC	1 cut(s)	970
BseBI	CCWGG	1 cut(s)	1131
BseDI	CCNNGG	3 cut(s)	66, 85, 975
BseGI	GGATG	2 cut(s)	532, 971
BseJI	GATNNNNATC	1 cut(s)	970
BseLI	CCNNNNNNNGG	3 cut(s)	91, 140, 962
BseMII	CTCAG	2 cut(s)	561, 622
BseNI	ACTGG	1 cut(s)	355
BseSI	GKGCMC	1 cut(s)	459
BseXI	GCAGC	1 cut(s)	569
BsgI	GTGCAG	1 cut(s)	698
Bsh1236I	CGCG	4 cut(s)	52, 277, 520, 1100
BshFI	GGCC	1 cut(s)	425
BsiHKAI	GWGCWC	1 cut(s)	459
BsiHKCI	CYCGRG	1 cut(s)	66
BsiSI	CCGG	2 cut(s)	67, 154
BslFI	GGGAC	1 cut(s)	458
BslI	CCNNNNNNNGG	3 cut(s)	91, 140, 962
BsmAI	GTCTC	3 cut(s)	100, 266, 1037
BsmBI	CGTCTC	1 cut(s)	266
BsmFI	GGGAC	1 cut(s)	458
BsnI	GGCC	1 cut(s)	425
Bso31I	GGTCTC	2 cut(s)	100, 1037
BsoBI	CYCGRG	1 cut(s)	66
Bsp1286I	GDGCHC	1 cut(s)	459
Bsp143I	GATC	1 cut(s)	336
Bsp1720I	GCTNAGC	1 cut(s)	570
BspACI	CCGC	5 cut(s)	99, 119, 374, 518, 1100
BspANI	GGCC	1 cut(s)	425
BspCNI	CTCAG	2 cut(s)	562, 621
BspFNI	CGCG	4 cut(s)	52, 277, 520, 1100
BspMAI	CTGCAG	1 cut(s)	559
BspMI	ACCTGC	1 cut(s)	86
BspQI	GCTCTTC	1 cut(s)	172
BspTI	CTTAAG	1 cut(s)	743
BspTNI	GGTCTC	2 cut(s)	100, 1037
BsrI	ACTGG	1 cut(s)	355
BssECI	CCNNGG	3 cut(s)	66, 85, 975
BssMI	GATC	1 cut(s)	336
BssSI	CACGAG	2 cut(s)	884, 1119
BssT1I	CCWWGG	2 cut(s)	85, 975
Bst2BI	CACGAG	2 cut(s)	884, 1119
Bst2UI	CCWGG	1 cut(s)	1131
Bst4CI	ACNGT	1 cut(s)	1028
Bst6I	CTCTTC	2 cut(s)	120, 172
BstAFI	CTTAAG	1 cut(s)	743
BstAPI	GCANNNNNTGC	1 cut(s)	1111
BstBAI	YACGTR	1 cut(s)	1066
BstC8I	GCNNGC	1 cut(s)	378
BstDEI	CTNAG	3 cut(s)	570, 608, 868
BstF5I	GGATG	2 cut(s)	532, 971
BstFNI	CGCG	4 cut(s)	52, 277, 520, 1100
BstKTI	GATC	1 cut(s)	339
BstMAI	GTCTC	3 cut(s)	100, 266, 1037
BstMBI	GATC	1 cut(s)	336
BstMWI	GCNNNNNNNGC	8 cut(s)	87, 191, 283, 566, 818, 968, 1111, 1140
BstNI	CCWGG	1 cut(s)	1131
BstNSI	RCATGY	1 cut(s)	653
BstSCI	CCNGG	3 cut(s)	65, 66, 1129
BstSFI	CTRYAG	2 cut(s)	555, 835
BstSLI	GKGCMC	1 cut(s)	459
BstUI	CGCG	4 cut(s)	52, 277, 520, 1100
BstV1I	GCAGC	1 cut(s)	569
BstV2I	GAAGAC	1 cut(s)	1080
BsuI	GTATCC	1 cut(s)	959
BsuRI	GGCC	1 cut(s)	425
BtsCI	GGATG	2 cut(s)	532, 971
BtsIMutI	CAGTG	1 cut(s)	875
BveI	ACCTGC	1 cut(s)	86
Cac8I	GCNNGC	1 cut(s)	378
Cfr13I	GGNCC	3 cut(s)	712, 1058, 1127
Cfr9I	CCCGGG	1 cut(s)	66
CseI	GACGC	3 cut(s)	41, 283, 1106
Csp6I	GTAC	2 cut(s)	951, 1029
CviAII	CATG	4 cut(s)	650, 909, 945, 1078
CviQI	GTAC	2 cut(s)	951, 1029
DdeI	CTNAG	3 cut(s)	570, 608, 868
DpnI	GATC	1 cut(s)	338
DpnII	GATC	1 cut(s)	336
Eam1104I	CTCTTC	2 cut(s)	120, 172
EarI	CTCTTC	2 cut(s)	120, 172
Eco130I	CCWWGG	2 cut(s)	85, 975
Eco147I	AGGCCT	1 cut(s)	425
Eco31I	GGTCTC	2 cut(s)	100, 1037
Eco47I	GGWCC	3 cut(s)	712, 1058, 1127
Eco57I	CTGAAG	1 cut(s)	649
Eco88I	CYCGRG	1 cut(s)	66
EcoRII	CCWGG	1 cut(s)	1129
EcoT14I	CCWWGG	2 cut(s)	85, 975
ErhI	CCWWGG	2 cut(s)	85, 975
Esp3I	CGTCTC	1 cut(s)	266
FaeI	CATG	4 cut(s)	653, 912, 948, 1081
FaqI	GGGAC	1 cut(s)	458
FatI	CATG	4 cut(s)	649, 908, 944, 1077
FauI	CCCGC	1 cut(s)	1093
Fnu4HI	GCNGC	1 cut(s)	558
FokI	GGATG	2 cut(s)	539, 958
Fsp4HI	GCNGC	1 cut(s)	558
FspBI	CTAG	4 cut(s)	251, 734, 899, 935
GluI	GCNGC	1 cut(s)	558
GsuI	CTGGAG	1 cut(s)	117
HaeIII	GGCC	1 cut(s)	425
HapII	CCGG	2 cut(s)	67, 154
HgaI	GACGC	3 cut(s)	41, 283, 1106
Hin1II	CATG	4 cut(s)	653, 912, 948, 1081
HincII	GTYRAC	2 cut(s)	56, 73
HindII	GTYRAC	2 cut(s)	56, 73
HindIII	AAGCTT	1 cut(s)	183
HinfI	GANTC	3 cut(s)	108, 361, 625
HpaII	CCGG	2 cut(s)	67, 154
HphI	GGTGA	3 cut(s)	365, 397, 1093
Hpy166II	GTNNAC	3 cut(s)	56, 73, 457
Hpy188I	TCNGA	3 cut(s)	107, 227, 1004
Hpy188III	TCNNGA	9 cut(s)	178, 289, 334, 533, 610, 788, 926, 1040, 1050
Hpy8I	GTNNAC	3 cut(s)	56, 73, 457
HpyAV	CCTTC	3 cut(s)	69, 524, 980
HpyCH4III	ACNGT	1 cut(s)	1028
HpyCH4IV	ACGT	2 cut(s)	563, 1065
HpyCH4V	TGCA	5 cut(s)	457, 557, 644, 679, 996
HpyF10VI	GCNNNNNNNGC	8 cut(s)	87, 191, 283, 566, 818, 968, 1111, 1140
HpyF3I	CTNAG	3 cut(s)	570, 608, 868
HpySE526I	ACGT	2 cut(s)	563, 1065
Hsp92II	CATG	4 cut(s)	653, 912, 948, 1081
Kzo9I	GATC	1 cut(s)	336
LguI	GCTCTTC	1 cut(s)	172
LmnI	GCTCC	3 cut(s)	86, 320, 818
Lsp1109I	GCAGC	1 cut(s)	569
LweI	GCATC	3 cut(s)	526, 782, 980
MaeI	CTAG	4 cut(s)	251, 734, 899, 935
MaeII	ACGT	2 cut(s)	563, 1065
MaeIII	GTNAC	1 cut(s)	509
MalI	GATC	1 cut(s)	338
MboI	GATC	1 cut(s)	336
MboII	GAAGA	9 cut(s)	54, 107, 159, 304, 326, 995, 1085, 1096, 1129
MhlI	GDGCHC	1 cut(s)	459
MluCI	AATT	3 cut(s)	228, 828, 1109
MluI	ACGCGT	1 cut(s)	50
MnlI	CCTC	9 cut(s)	258, 415, 517, 649, 698, 880, 883, 998, 1057
MroXI	GAANNNNTTC	2 cut(s)	601, 798
MseI	TTAA	2 cut(s)	342, 744
MslI	CAYNNNNRTG	1 cut(s)	452
MspA1I	CMGCKG	2 cut(s)	581, 806
MspCI	CTTAAG	1 cut(s)	743
MspI	CCGG	2 cut(s)	67, 154
MspR9I	CCNGG	3 cut(s)	67, 68, 1131
MvaI	CCWGG	1 cut(s)	1131
MvnI	CGCG	4 cut(s)	52, 277, 520, 1100
MwoI	GCNNNNNNNGC	8 cut(s)	87, 191, 283, 566, 818, 968, 1111, 1140
NciI	CCSGG	2 cut(s)	67, 68
NdeII	GATC	1 cut(s)	336
NlaIII	CATG	4 cut(s)	653, 912, 948, 1081
NspI	RCATGY	1 cut(s)	653
PaqCI	CACCTGC	1 cut(s)	86
PceI	AGGCCT	1 cut(s)	425
PciI	ACATGT	1 cut(s)	649
PciSI	GCTCTTC	1 cut(s)	172
PdmI	GAANNNNTTC	2 cut(s)	601, 798
PfeI	GAWTC	3 cut(s)	108, 361, 625
PkrI	GCNGC	1 cut(s)	559
Ppu21I	YACGTR	1 cut(s)	1066
PscI	ACATGT	1 cut(s)	649
Psp6I	CCWGG	1 cut(s)	1129
PspGI	CCWGG	1 cut(s)	1129
PspPI	GGNCC	3 cut(s)	712, 1058, 1127
PstI	CTGCAG	1 cut(s)	559
PvuII	CAGCTG	2 cut(s)	581, 806
RsaI	GTAC	2 cut(s)	952, 1030
RsaNI	GTAC	2 cut(s)	951, 1029
RseI	CAYNNNNRTG	1 cut(s)	452
SapI	GCTCTTC	1 cut(s)	172
SaqAI	TTAA	2 cut(s)	342, 744
SatI	GCNGC	1 cut(s)	558
Sau3AI	GATC	1 cut(s)	336
Sau96I	GGNCC	3 cut(s)	712, 1058, 1127
ScrFI	CCNGG	3 cut(s)	67, 68, 1131
SduI	GDGCHC	1 cut(s)	459
SfaNI	GCATC	3 cut(s)	526, 782, 980
SfcI	CTRYAG	2 cut(s)	555, 835
SinI	GGWCC	3 cut(s)	712, 1058, 1127
SmaI	CCCGGG	1 cut(s)	68
SmiMI	CAYNNNNRTG	1 cut(s)	452
SmlI	CTYRAG	6 cut(s)	261, 287, 743, 813, 1040, 1048
SmoI	CTYRAG	6 cut(s)	261, 287, 743, 813, 1040, 1048
Sse9I	AATT	3 cut(s)	228, 828, 1109
SseBI	AGGCCT	1 cut(s)	425
SsiI	CCGC	5 cut(s)	99, 119, 374, 518, 1100
SspMI	CTAG	4 cut(s)	251, 734, 899, 935
StuI	AGGCCT	1 cut(s)	425
StyD4I	CCNGG	3 cut(s)	65, 66, 1129
StyI	CCWWGG	2 cut(s)	85, 975
TaaI	ACNGT	1 cut(s)	1028
TaiI	ACGT	2 cut(s)	566, 1068
TaqI	TCGA	3 cut(s)	333, 532, 789
TaqII	GACCGA	1 cut(s)	82
TasI	AATT	3 cut(s)	228, 828, 1109
TfiI	GAWTC	3 cut(s)	108, 361, 625
Tru1I	TTAA	2 cut(s)	342, 744
Tru9I	TTAA	2 cut(s)	342, 744
TscAI	CASTG	1 cut(s)	882
TseI	GCWGC	1 cut(s)	557
TspDTI	ATGAA	5 cut(s)	317, 479, 807, 897, 1086
TspMI	CCCGGG	1 cut(s)	66
TspRI	CASTG	1 cut(s)	882
Vha464I	CTTAAG	1 cut(s)	743
VneI	GTGCAC	1 cut(s)	455
VpaK11BI	GGWCC	3 cut(s)	712, 1058, 1127
XceI	RCATGY	1 cut(s)	653
XmaI	CCCGGG	1 cut(s)	66
XmnI	GAANNNNTTC	2 cut(s)	601, 798
XspI	CTAG	4 cut(s)	251, 734, 899, 935

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.