Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004391

Physical Location & Seq

Genomic Coordinates Forward (+)

246960 .. 249781

Gene Structure 2822 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1004100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1167 bp

ATGGATGCTTTTTGTTCTTCTTCTTCCTGCTCCTCGTCTCTTTTGACCAAGCAGGCGCTTCCTTCATGCTCTTCAATCAACTTCGCAGCAGGAGTTCGCACCGTCTCAGTCCTCCACTTGTCCATGCCCTCCCTCAAGCCTCGAGCTTTTCCCAACTCTATCAGAAAACACAGTCTAATTGGGCTCCCAAGGGCCTCCGTCGCTGTTGAGCAACAGACCCAGAAGTCCAAACTCGCCCTAATCAAAATTGGCACCCGGGGAAGCCCATTAGCACTTGCTCAGGCTTACGAGACAAGAGAGAAACTCATGGCATCGCACACCGAGCTAGCTGAAGAGGGCGCAATTCAGATTGTTATCATAAAAACTACAGGTGATAAAATACTGAGCCAGCCCCTTGCAGACATTGGCGGGAAGGGTTTGTTCACCAAGGAAATAGATGAGGCACTCATCAATGGCGATGTTGACATTGCTGTCCACTCAATGAAAGACGTGCCTACCTACTTACCCGAGAAGACAATTCTTCCTTGCAACCTTCCGCGAGAGGATGTCCGAGATGCATTTATATCTTTAACTGCATCTTCTCTTGCAGATTTGCCAGCTGGAAGCACTGTTGGTACTGCTTCACTTAGAAGAAAGTCACAGATACTCCATAGATTTCCAACACTCAATGTGCAGGAGAATTTTCGGGGTAACGTCCAGACACGGCTGAAAAAGCTTAATGAAAAGGTGGTCGAAGCAACCTTATTAGCATTAGCTGGACTCAAACGCTTAGATATGACAGAAAATGTGACTTCAATTCTTTCACTAGATGAAATGCTTCCAGCAGTTGCCCAAGGGGCAATTGGAATAGCCTGCCGAACCAATGACGATAAAATGGCTAATTACATAGCGTCCTTGAATCATGAGGAAACAAGACTAGCAGTTGCATGCGAGAGGTCGTTCCTTCTGACCCTGGATGGGTCTTGTCGAACTCCTATTGCTGGATATGCTAGCAAAGATGAGGATGGTAATTGCATATTCAAAGGGTTGGTGGCTTCCCCTGATGGAACCCGTGTACTTGAAACTTCTAGGAAAGGCCCGTATGCTTTTCAAGATATGATCGACATGGGTAAGGATGCTGGCCAGGAACTTCTTTCACGTGCAGGTCCAGGTTTTTTTGGTATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004418 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006778 GO:0006779 GO:0006783 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009451 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009941 GO:0009987 GO:0015994 GO:0015995 GO:0016070 GO:0016740 GO:0016765 GO:0018130 GO:0019438 GO:0031967 GO:0031975 GO:0033013 GO:0033014 GO:0034641 GO:0042168 GO:0042440 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046148 GO:0046483 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0051704 GO:0051707 GO:0071704 GO:0090304 GO:0098542 GO:1900865 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120

Pfam Domains

Protein Families

Porphobil_deam Porphobil_deamC

Protein Analysis

389

Amino Acids

41.67

Weight (kDa)

8.06

Isoelectric Point (pI)

35.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Porphobil_deam	PF01379	81 - 293	1.3e-78	Porphobilinogen deaminase, dipyromethane cofactor binding domain
Porphobil_deamC	PF03900	306 - 378	1.1e-13	Porphobilinogen deaminase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004391)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08280
fragaria_vesca	FvH4_4g37020 FvH4_7g04700
malus_domestica	MD13G1004100.v1.1 MD16G1003400.v1.1
prunus_persica	Prupe.1G348400_v2.0.a1 Prupe.1G348400_v2.0.a1 Prupe.1G348400_v2.0.a1
pyrus_communis	pycom13g00340 pycom16g00240
rosa_chinensis	RchiOBHm_Chr1g0330101 RchiOBHm_Chr4g0446801
rosa_laevigata	RLG00000005629 RLG00000029832
rosa_multiflora	Rmu_co8512413.1_g000001 Rmu_sc0001753.1_g000022 Rmu_sc0004673.1_g000003
rosa_roxburghii	Rroxscaffold_4G00320380 Rroxscaffold_5G00387180
rosa_rugosa	Rorug01G0085800 Rorug01G0085900 Rorug04G0374900
rosa_samantha	Rh1AG104400 Rh1CG100000 Rh1DG107300 Rh1DG108400 Rh4AG436200 Rh4BG445300 Rh4CG462600 Rh4DG444000
rosa_wichuraiana	Rw0G015900 Rw4G037310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	223, 470
Acc36I	ACCTGC	1 cut(s)	1133
AccB1I	GGYRCC	1 cut(s)	251
AccII	CGCG	1 cut(s)	538
AciI	CCGC	2 cut(s)	408, 536
AcoI	YGGCCR	1 cut(s)	1120
AcsI	RAATTY	1 cut(s)	679
AcuI	CTGAAG	1 cut(s)	351
AcvI	CACGTG	1 cut(s)	1139
AfaI	GTAC	2 cut(s)	616, 1056
AfiI	CCNNNNNNNGG	2 cut(s)	957, 980
AgsI	TTSAA	6 cut(s)	75, 795, 898, 1021, 1061, 1091
AjiI	CACGTC	1 cut(s)	490
AjnI	CCWGG	3 cut(s)	951, 1122, 1147
AluBI	AGCT	6 cut(s)	146, 325, 329, 599, 715, 755
AluI	AGCT	6 cut(s)	146, 325, 329, 599, 715, 755
Alw26I	GTCTC	3 cut(s)	42, 109, 284
Ama87I	CYCGRG	3 cut(s)	141, 255, 506
AoxI	GGCC	3 cut(s)	192, 1075, 1120
ApeKI	GCWGC	1 cut(s)	86
ApoI	RAATTY	1 cut(s)	679
Asp700I	GAANNNNTTC	1 cut(s)	816
AspLEI	GCGC	2 cut(s)	58, 341
AspS9I	GGNCC	3 cut(s)	192, 1076, 1145
AsuC2I	CCSGG	2 cut(s)	256, 257
AsuHPI	GGTGA	2 cut(s)	383, 415
AsuNHI	GCTAGC	2 cut(s)	325, 989
AvaI	CYCGRG	3 cut(s)	141, 255, 506
AvaII	GGWCC	1 cut(s)	1145
BalI	TGGCCA	1 cut(s)	1122
BanI	GGYRCC	1 cut(s)	251
BanII	GRGCYC	1 cut(s)	186
BbrPI	CACGTG	1 cut(s)	1139
BbsI	GAAGAC	1 cut(s)	518
BbvI	GCAGC	1 cut(s)	98
BccI	CCATC	3 cut(s)	950, 998, 1037
BceAI	ACGGC	1 cut(s)	719
BciT130I	CCWGG	3 cut(s)	953, 1124, 1149
BcnI	CCSGG	2 cut(s)	256, 257
BcoDI	GTCTC	3 cut(s)	42, 109, 284
BfaI	CTAG	5 cut(s)	326, 806, 917, 990, 1068
BfmI	CTRYAG	1 cut(s)	366
BfoI	RGCGCY	1 cut(s)	59
BfuAI	ACCTGC	1 cut(s)	1133
BglI	GCCNNNNNGGC	1 cut(s)	836
BisI	GCNGC	1 cut(s)	87
BlsI	GCNGC	1 cut(s)	88
Bme1390I	CCNGG	5 cut(s)	256, 257, 953, 1124, 1149
Bme18I	GGWCC	1 cut(s)	1145
BmeT110I	CYCGRG	3 cut(s)	141, 255, 506
BmgBI	CACGTC	1 cut(s)	490
BmgT120I	GGNCC	3 cut(s)	192, 1076, 1145
BmiI	GGNNCC	3 cut(s)	185, 253, 1048
BmrFI	CCNGG	5 cut(s)	256, 257, 953, 1124, 1149
BmsI	GCATC	4 cut(s)	320, 544, 584, 1105
BmtI	GCTAGC	2 cut(s)	329, 993
BpiI	GAAGAC	1 cut(s)	518
BplI	GAGNNNNNCTC	2 cut(s)	288, 320
Bpu10I	CCTNAGC	1 cut(s)	279
BpuEI	CTTGAG	1 cut(s)	119
BpuMI	CCSGG	2 cut(s)	256, 257
BsaAI	YACGTR	1 cut(s)	1139
BsaBI	GATNNNNATC	1 cut(s)	353
BsaJI	CCNNGG	6 cut(s)	188, 255, 256, 426, 832, 951
BsaXI	ACNNNNNCTCC	2 cut(s)	630, 660
Bsc4I	CCNNNNNNNGG	2 cut(s)	957, 980
Bse3DI	GCAATG	1 cut(s)	465
Bse8I	GATNNNNATC	1 cut(s)	353
BseBI	CCWGG	3 cut(s)	953, 1124, 1149
BseDI	CCNNGG	6 cut(s)	188, 255, 256, 426, 832, 951
BseGI	GGATG	5 cut(s)	10, 550, 961, 1009, 1120
BseJI	GATNNNNATC	1 cut(s)	353
BseLI	CCNNNNNNNGG	2 cut(s)	957, 980
BseMI	GCAATG	1 cut(s)	465
BseMII	CTCAG	3 cut(s)	120, 293, 374
BseRI	GAGGAG	1 cut(s)	22
BseXI	GCAGC	1 cut(s)	98
BsgI	GTGCAG	2 cut(s)	692, 1161
Bsh1236I	CGCG	1 cut(s)	538
BshFI	GGCC	3 cut(s)	194, 1077, 1122
BshNI	GGYRCC	1 cut(s)	251
BsiHKCI	CYCGRG	3 cut(s)	141, 255, 506
BsiSI	CCGG	1 cut(s)	256
BslI	CCNNNNNNNGG	2 cut(s)	957, 980
BsmAI	GTCTC	3 cut(s)	42, 109, 284
BsmBI	CGTCTC	2 cut(s)	42, 109
BsnI	GGCC	3 cut(s)	194, 1077, 1122
BsoBI	CYCGRG	3 cut(s)	141, 255, 506
Bsp1286I	GDGCHC	1 cut(s)	186
Bsp143I	GATC	1 cut(s)	1098
BspACI	CCGC	2 cut(s)	408, 536
BspANI	GGCC	3 cut(s)	194, 1077, 1122
BspCNI	CTCAG	3 cut(s)	119, 292, 375
BspFNI	CGCG	1 cut(s)	538
BspHI	TCATGA	1 cut(s)	901
BspLI	GGNNCC	3 cut(s)	185, 253, 1048
BspMI	ACCTGC	1 cut(s)	1133
BspOI	GCTAGC	2 cut(s)	329, 993
BspQI	GCTCTTC	1 cut(s)	76
BspT107I	GGYRCC	1 cut(s)	251
BsrDI	GCAATG	1 cut(s)	465
BssECI	CCNNGG	6 cut(s)	188, 255, 256, 426, 832, 951
BssMI	GATC	1 cut(s)	1098
BssT1I	CCWWGG	3 cut(s)	188, 426, 832
Bst2UI	CCWGG	3 cut(s)	953, 1124, 1149
Bst4CI	ACNGT	3 cut(s)	103, 173, 610
Bst6I	CTCTTC	2 cut(s)	76, 327
BstBAI	YACGTR	1 cut(s)	1139
BstC8I	GCNNGC	8 cut(s)	54, 327, 389, 597, 853, 928, 991, 1120
BstDEI	CTNAG	5 cut(s)	106, 279, 383, 626, 769
BstF5I	GGATG	5 cut(s)	10, 550, 961, 1009, 1120
BstFNI	CGCG	1 cut(s)	538
BstH2I	RGCGCY	1 cut(s)	59
BstHHI	GCGC	2 cut(s)	58, 341
BstKTI	GATC	1 cut(s)	1101
BstMAI	GTCTC	3 cut(s)	42, 109, 284
BstMBI	GATC	1 cut(s)	1098
BstMWI	GCNNNNNNNGC	5 cut(s)	200, 322, 712, 836, 986
BstNI	CCWGG	3 cut(s)	953, 1124, 1149
BstNSI	RCATGY	1 cut(s)	930
BstSCI	CCNGG	5 cut(s)	254, 255, 951, 1122, 1147
BstSFI	CTRYAG	1 cut(s)	366
BstUI	CGCG	1 cut(s)	538
BstV1I	GCAGC	1 cut(s)	98
BstV2I	GAAGAC	1 cut(s)	518
BsuRI	GGCC	3 cut(s)	194, 1077, 1122
BtgZI	GCGATG	2 cut(s)	297, 471
BtrI	CACGTC	1 cut(s)	490
BtsCI	GGATG	5 cut(s)	10, 550, 961, 1009, 1120
BtsIMutI	CAGTG	1 cut(s)	606
BveI	ACCTGC	1 cut(s)	1133
Cac8I	GCNNGC	8 cut(s)	54, 327, 389, 597, 853, 928, 991, 1120
CciI	TCATGA	1 cut(s)	901
CfoI	GCGC	2 cut(s)	58, 341
Cfr13I	GGNCC	3 cut(s)	192, 1076, 1145
Cfr9I	CCCGGG	1 cut(s)	255
CseI	GACGC	1 cut(s)	879
Csp6I	GTAC	2 cut(s)	615, 1055
CviAII	CATG	6 cut(s)	66, 124, 307, 902, 927, 1105
CviQI	GTAC	2 cut(s)	615, 1055
DdeI	CTNAG	5 cut(s)	106, 279, 383, 626, 769
DpnI	GATC	1 cut(s)	1100
DpnII	GATC	1 cut(s)	1098
DrdI	GACNNNNNNGTC	2 cut(s)	223, 470
DseDI	GACNNNNNNGTC	2 cut(s)	223, 470
EaeI	YGGCCR	1 cut(s)	1120
Eam1104I	CTCTTC	2 cut(s)	76, 327
EarI	CTCTTC	2 cut(s)	76, 327
Eco130I	CCWWGG	3 cut(s)	188, 426, 832
Eco24I	GRGCYC	1 cut(s)	186
Eco47I	GGWCC	1 cut(s)	1145
Eco57I	CTGAAG	1 cut(s)	351
Eco72I	CACGTG	1 cut(s)	1139
Eco88I	CYCGRG	3 cut(s)	141, 255, 506
EcoO109I	RGGNCCY	1 cut(s)	192
EcoRII	CCWGG	3 cut(s)	951, 1122, 1147
EcoT14I	CCWWGG	3 cut(s)	188, 426, 832
EcoT22I	ATGCAT	1 cut(s)	559
EcoT38I	GRGCYC	1 cut(s)	186
ErhI	CCWWGG	3 cut(s)	188, 426, 832
Esp3I	CGTCTC	2 cut(s)	42, 109
FaeI	CATG	6 cut(s)	69, 127, 310, 905, 930, 1108
FatI	CATG	6 cut(s)	65, 123, 306, 901, 926, 1104
FauI	CCCGC	1 cut(s)	401
Fnu4HI	GCNGC	1 cut(s)	87
FokI	GGATG	5 cut(s)	17, 557, 968, 1016, 1127
FriOI	GRGCYC	1 cut(s)	186
Fsp4HI	GCNGC	1 cut(s)	87
FspBI	CTAG	5 cut(s)	326, 806, 917, 990, 1068
GlaI	GCGC	2 cut(s)	57, 340
GluI	GCNGC	1 cut(s)	87
HaeII	RGCGCY	1 cut(s)	59
HaeIII	GGCC	3 cut(s)	194, 1077, 1122
HapII	CCGG	1 cut(s)	256
HgaI	GACGC	1 cut(s)	879
HhaI	GCGC	2 cut(s)	58, 341
Hin1II	CATG	6 cut(s)	69, 127, 310, 905, 930, 1108
Hin6I	GCGC	2 cut(s)	56, 339
HinP1I	GCGC	2 cut(s)	56, 339
HincII	GTYRAC	1 cut(s)	463
HindII	GTYRAC	1 cut(s)	463
HindIII	AAGCTT	1 cut(s)	713
HinfI	GANTC	2 cut(s)	759, 898
HpaII	CCGG	1 cut(s)	256
HphI	GGTGA	2 cut(s)	383, 415
Hpy166II	GTNNAC	4 cut(s)	423, 463, 475, 1055
Hpy188I	TCNGA	4 cut(s)	164, 348, 551, 948
Hpy188III	TCNNGA	3 cut(s)	697, 902, 1091
Hpy8I	GTNNAC	4 cut(s)	423, 463, 475, 1055
Hpy99I	CGWCG	1 cut(s)	203
HpyAV	CCTTC	4 cut(s)	72, 406, 542, 953
HpyCH4III	ACNGT	3 cut(s)	103, 173, 610
HpyCH4IV	ACGT	3 cut(s)	489, 693, 1138
HpyCH4V	TGCA	9 cut(s)	398, 528, 557, 575, 587, 673, 926, 1014, 1142
HpyF10VI	GCNNNNNNNGC	5 cut(s)	200, 322, 712, 836, 986
HpyF3I	CTNAG	5 cut(s)	106, 279, 383, 626, 769
HpySE526I	ACGT	3 cut(s)	489, 693, 1138
Hsp92II	CATG	6 cut(s)	69, 127, 310, 905, 930, 1108
HspAI	GCGC	2 cut(s)	56, 339
Kzo9I	GATC	1 cut(s)	1098
LguI	GCTCTTC	1 cut(s)	76
LmnI	GCTCC	2 cut(s)	35, 189
Lsp1109I	GCAGC	1 cut(s)	98
LweI	GCATC	4 cut(s)	320, 544, 584, 1105
MaeI	CTAG	5 cut(s)	326, 806, 917, 990, 1068
MaeII	ACGT	3 cut(s)	489, 693, 1138
MaeIII	GTNAC	3 cut(s)	636, 689, 787
MalI	GATC	1 cut(s)	1100
MboI	GATC	1 cut(s)	1098
MboII	GAAGA	9 cut(s)	9, 12, 15, 63, 344, 512, 523, 570, 642
MfeI	CAATTG	1 cut(s)	840
MhlI	GDGCHC	1 cut(s)	186
MlsI	TGGCCA	1 cut(s)	1122
MluCI	AATT	9 cut(s)	177, 246, 342, 516, 679, 795, 840, 880, 1009
MluNI	TGGCCA	1 cut(s)	1122
MlyI	GAGTC	1 cut(s)	753
MmeI	TCCRAC	1 cut(s)	683
Mox20I	TGGCCA	1 cut(s)	1122
Mph1103I	ATGCAT	1 cut(s)	559
MroXI	GAANNNNTTC	1 cut(s)	816
MscI	TGGCCA	1 cut(s)	1122
MseI	TTAA	3 cut(s)	569, 717, 1165
Msp20I	TGGCCA	1 cut(s)	1122
MspA1I	CMGCKG	1 cut(s)	599
MspI	CCGG	1 cut(s)	256
MspR9I	CCNGG	5 cut(s)	256, 257, 953, 1124, 1149
MunI	CAATTG	1 cut(s)	840
MvaI	CCWGG	3 cut(s)	953, 1124, 1149
MvnI	CGCG	1 cut(s)	538
MwoI	GCNNNNNNNGC	5 cut(s)	200, 322, 712, 836, 986
NciI	CCSGG	2 cut(s)	256, 257
NdeII	GATC	1 cut(s)	1098
NheI	GCTAGC	2 cut(s)	325, 989
NlaIII	CATG	6 cut(s)	69, 127, 310, 905, 930, 1108
NlaIV	GGNNCC	3 cut(s)	185, 253, 1048
NmuCI	GTSAC	2 cut(s)	636, 787
NsiI	ATGCAT	1 cut(s)	559
NspI	RCATGY	1 cut(s)	930
PaeI	GCATGC	1 cut(s)	930
PaeR7I	CTCGAG	1 cut(s)	141
PagI	TCATGA	1 cut(s)	901
PciSI	GCTCTTC	1 cut(s)	76
PdmI	GAANNNNTTC	1 cut(s)	816
PfeI	GAWTC	1 cut(s)	898
PkrI	GCNGC	1 cut(s)	88
PleI	GAGTC	1 cut(s)	753
PmaCI	CACGTG	1 cut(s)	1139
PmlI	CACGTG	1 cut(s)	1139
PpsI	GAGTC	1 cut(s)	753
Ppu21I	YACGTR	1 cut(s)	1139
Psp6I	CCWGG	3 cut(s)	951, 1122, 1147
PspCI	CACGTG	1 cut(s)	1139
PspGI	CCWGG	3 cut(s)	951, 1122, 1147
PspN4I	GGNNCC	3 cut(s)	185, 253, 1048
PspPI	GGNCC	3 cut(s)	192, 1076, 1145
PspXI	VCTCGAGB	1 cut(s)	141
PvuII	CAGCTG	1 cut(s)	599
RsaI	GTAC	2 cut(s)	616, 1056
RsaNI	GTAC	2 cut(s)	615, 1055
SapI	GCTCTTC	1 cut(s)	76
SaqAI	TTAA	3 cut(s)	569, 717, 1165
SatI	GCNGC	1 cut(s)	87
Sau3AI	GATC	1 cut(s)	1098
Sau96I	GGNCC	3 cut(s)	192, 1076, 1145
SchI	GAGTC	1 cut(s)	753
ScrFI	CCNGG	5 cut(s)	256, 257, 953, 1124, 1149
SduI	GDGCHC	1 cut(s)	186
SfaNI	GCATC	4 cut(s)	320, 544, 584, 1105
SfcI	CTRYAG	1 cut(s)	366
Sfr274I	CTCGAG	1 cut(s)	141
SinI	GGWCC	1 cut(s)	1145
SlaI	CTCGAG	1 cut(s)	141
SmaI	CCCGGG	1 cut(s)	257
SmlI	CTYRAG	2 cut(s)	134, 141
SmoI	CTYRAG	2 cut(s)	134, 141
SphI	GCATGC	1 cut(s)	930
Sse9I	AATT	9 cut(s)	177, 246, 342, 516, 679, 795, 840, 880, 1009
SsiI	CCGC	2 cut(s)	408, 536
SspMI	CTAG	5 cut(s)	326, 806, 917, 990, 1068
StyD4I	CCNGG	5 cut(s)	254, 255, 951, 1122, 1147
StyI	CCWWGG	3 cut(s)	188, 426, 832
TaaI	ACNGT	3 cut(s)	103, 173, 610
TaiI	ACGT	3 cut(s)	492, 696, 1141
TaqI	TCGA	4 cut(s)	142, 732, 967, 1101
TasI	AATT	9 cut(s)	177, 246, 342, 516, 679, 795, 840, 880, 1009
TatI	WGTACW	1 cut(s)	1054
TfiI	GAWTC	1 cut(s)	898
Tru1I	TTAA	3 cut(s)	569, 717, 1165
Tru9I	TTAA	3 cut(s)	569, 717, 1165
TscAI	CASTG	1 cut(s)	613
TseFI	GTSAC	2 cut(s)	636, 787
TseI	GCWGC	1 cut(s)	86
Tsp45I	GTSAC	2 cut(s)	636, 787
TspDTI	ATGAA	4 cut(s)	54, 497, 735, 825
TspGWI	ACGGA	1 cut(s)	187
TspMI	CCCGGG	1 cut(s)	255
TspRI	CASTG	1 cut(s)	613
VpaK11BI	GGWCC	1 cut(s)	1145
XapI	RAATTY	1 cut(s)	679
XceI	RCATGY	1 cut(s)	930
XcmI	CCANNNNNNNNNTGG	1 cut(s)	839
XhoI	CTCGAG	1 cut(s)	141
XmaI	CCCGGG	1 cut(s)	255
XmnI	GAANNNNTTC	1 cut(s)	816
XspI	CTAG	5 cut(s)	326, 806, 917, 990, 1068
Zsp2I	ATGCAT	1 cut(s)	559

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.