AT5G12040

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010419
Physical Location & Seq
Forward (+)
3884949 .. 3888077
3129 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G12040.2

Sequence Viewer

Length: 1110 bp
ATGAAGTCAGCAATTTCATCGTCACTCTTCTTCAATTCGAAGAATCTTTTAAACCCTAATCCTCTTTCTCGCTTCATTTCTCTCAAATCTAACTTCCTCCCTAAATTATCTCCGAGATCGATCACTAGTCACACCTTGAAGCTCCCATCTTCGTCAACCTCAGCTTTAAGATCCATTTCCTCTTCCATGGCTTCTTCTTTCAACCCTGAACAAGCTAGAGTTCCCTCTGCTCTTCCTCTCCCAGCTCCTCCGTTGACCAAATTCAACATCGGATTGTGTCAGCTATCTGTTACATCTGACAAAAAGAGAAACATCTCTCATGCTAAAAAAGCCATTGAAGAAGCTGCTTCTAAAGGAGCTAAGCTTGTTCTCTTACCCGAAATTTGGAACAGTCCGTATTCCAATGATAGTTTTCCAGTTTATGCGGAGGAGATTGATGCAGGTGGTGATGCTTCTCCTTCAACGGCAATGCTTTCTGAAGTTTCCAAACGTCTCAAGATTACAATCATTGGTGGATCTATACCAGAAAGAGTTGGAGATCGTTTGTATAACACTTGCTGTGTCTTTGGTTCCGATGGAGAGCTAAAAGCTAAGCATCGGAAGATACATTTATTTGATATAGACATTCCCGGGAAGATTACTTTTATGGAATCCAAAACTCTTACTGCTGGAGAGACACCAACAATCGTTGACACAGATGTAGGGCGTATTGGAATAGGCATCTGTTATGATATCAGGTTCCAGGAGTTAGCTATGATATATGCTGCAAGAGGGGCTCATTTGCTGTGCTACCCGGGAGCCTTTAACATGACAACTGGACCATTGCATTGGGAATTACTACAAAGGGCCAGGGCTACGGATAATCAGTTATATGTGGCGACATGCTCACCTGCCAGAGATTCAGGAGCTGGCTACACTGCTTGGGGGCACTCAACACTCGTTGGGCCTTTTGGAGAAGTACTAGCAACGACTGAGCATGAGGAGGCCATTATCATAGCAGAGATTGATTACTCTATCCTTGAACAACGAAGGACTAGCCTTCCATTGAATAGGCAGCGGCGGGGAGATCTTTACCAGCTTGTAGACGTACAGCGCTTAGACTCTAAATGA

Protein Analysis

369

Amino Acids

40.33

Weight (kDa)

8.79

Isoelectric Point (pI)

44.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 90 - 290 1.8e-51 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 431, 898
Acc36I ACCTGC 2 cut(s) 431, 898
AccI GTMKAC 1 cut(s) 1083
AciI CCGC 3 cut(s) 425, 1057, 1060
AclWI GGATC 2 cut(s) 165, 523
AcsI RAATTY 2 cut(s) 260, 381
AcuI CTGAAG 1 cut(s) 498
AfaI GTAC 2 cut(s) 960, 1089
AfeI AGCGCT 1 cut(s) 1094
AfiI CCNNNNNNNGG 1 cut(s) 384
AgsI TTSAA 8 cut(s) 34, 139, 202, 265, 338, 462, 1022, 1048
AhlI ACTAGT 1 cut(s) 125
AjnI CCWGG 2 cut(s) 741, 848
Alw26I GTCTC 2 cut(s) 497, 668
AlwI GGATC 2 cut(s) 165, 523
AlwNI CAGNNNCTG 1 cut(s) 908
Ama87I CYCGRG 2 cut(s) 629, 793
Aor51HI AGCGCT 1 cut(s) 1094
AoxI GGCC 3 cut(s) 846, 944, 984
ApeKI GCWGC 3 cut(s) 344, 764, 1054
ApoI RAATTY 2 cut(s) 260, 381
AspLEI GCGC 1 cut(s) 1095
AspS9I GGNCC 3 cut(s) 818, 846, 944
AsuC2I CCSGG 4 cut(s) 630, 631, 794, 795
AsuHPI GGTGA 2 cut(s) 458, 879
AsuII TTCGAA 1 cut(s) 38
AvaI CYCGRG 2 cut(s) 629, 793
AvaII GGWCC 1 cut(s) 818
BaeGI GKGCMC 1 cut(s) 930
BanII GRGCYC 1 cut(s) 778
BbvCI CCTCAGC 1 cut(s) 160
BbvI GCAGC 3 cut(s) 331, 751, 1066
BccI CCATC 2 cut(s) 154, 569
BceAI ACGGC 1 cut(s) 480
BcgI CGANNNNNNTGC 1 cut(s) 34
BciT130I CCWGG 2 cut(s) 743, 850
BcnI CCSGG 4 cut(s) 630, 631, 794, 795
BcoDI GTCTC 2 cut(s) 497, 668
BcuI ACTAGT 1 cut(s) 125
BfaI CTAG 4 cut(s) 126, 216, 962, 1035
BfoI RGCGCY 1 cut(s) 1096
BfuAI ACCTGC 2 cut(s) 431, 898
BglII AGATCT 1 cut(s) 1066
BisI GCNGC 4 cut(s) 345, 765, 1055, 1058
BlpI GCTNAGC 2 cut(s) 360, 591
BlsI GCNGC 4 cut(s) 346, 766, 1056, 1059
BmcAI AGTACT 1 cut(s) 960
Bme1390I CCNGG 6 cut(s) 630, 631, 743, 794, 795, 850
Bme18I GGWCC 1 cut(s) 818
BmeT110I CYCGRG 2 cut(s) 629, 793
BmgT120I GGNCC 3 cut(s) 818, 846, 944
BmiI GGNNCC 3 cut(s) 571, 740, 799
BmrFI CCNGG 6 cut(s) 630, 631, 743, 794, 795, 850
BmsI GCATC 4 cut(s) 427, 439, 604, 729
BpmI CTGGAG 1 cut(s) 690
Bpu10I CCTNAGC 1 cut(s) 160
Bpu1102I GCTNAGC 2 cut(s) 360, 591
Bpu14I TTCGAA 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 479
BpuMI CCSGG 4 cut(s) 630, 631, 794, 795
Bsa29I ATCGAT 1 cut(s) 119
BsaBI GATNNNNATC 1 cut(s) 503
BsaJI CCNNGG 4 cut(s) 186, 629, 793, 849
Bsc4I CCNNNNNNNGG 1 cut(s) 384
Bse1I ACTGG 2 cut(s) 416, 820
Bse3DI GCAATG 2 cut(s) 474, 821
Bse8I GATNNNNATC 1 cut(s) 503
BseBI CCWGG 2 cut(s) 743, 850
BseCI ATCGAT 1 cut(s) 119
BseDI CCNNGG 4 cut(s) 186, 629, 793, 849
BseJI GATNNNNATC 1 cut(s) 503
BseLI CCNNNNNNNGG 1 cut(s) 384
BseMI GCAATG 2 cut(s) 474, 821
BseMII CTCAG 2 cut(s) 174, 963
BseNI ACTGG 2 cut(s) 416, 820
BseRI GAGGAG 3 cut(s) 237, 443, 995
BseSI GKGCMC 1 cut(s) 930
BseXI GCAGC 3 cut(s) 331, 751, 1066
BseYI CCCAGC 1 cut(s) 241
BshFI GGCC 3 cut(s) 848, 946, 986
BshVI ATCGAT 1 cut(s) 119
BsiHKCI CYCGRG 2 cut(s) 629, 793
BsiSI CCGG 2 cut(s) 630, 794
BslI CCNNNNNNNGG 1 cut(s) 384
BsmAI GTCTC 2 cut(s) 497, 668
BsmBI CGTCTC 1 cut(s) 497
BsnI GGCC 3 cut(s) 848, 946, 986
BsoBI CYCGRG 2 cut(s) 629, 793
Bsp119I TTCGAA 1 cut(s) 38
Bsp1286I GDGCHC 2 cut(s) 778, 930
Bsp143I GATC 6 cut(s) 116, 120, 170, 515, 538, 1066
Bsp1720I GCTNAGC 2 cut(s) 360, 591
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 3 cut(s) 425, 1057, 1060
BspANI GGCC 3 cut(s) 848, 946, 986
BspCNI CTCAG 2 cut(s) 173, 964
BspDI ATCGAT 1 cut(s) 119
BspLI GGNNCC 3 cut(s) 571, 740, 799
BspMI ACCTGC 2 cut(s) 431, 898
BspPI GGATC 2 cut(s) 165, 523
BspQI GCTCTTC 1 cut(s) 237
BspT104I TTCGAA 1 cut(s) 38
BsrDI GCAATG 2 cut(s) 474, 821
BsrI ACTGG 2 cut(s) 416, 820
BssECI CCNNGG 4 cut(s) 186, 629, 793, 849
BssMI GATC 6 cut(s) 116, 120, 170, 515, 538, 1066
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 743, 850
Bst4CI ACNGT 1 cut(s) 392
Bst6I CTCTTC 3 cut(s) 32, 187, 237
BstBI TTCGAA 1 cut(s) 38
BstC8I GCNNGC 1 cut(s) 910
BstDEI CTNAG 5 cut(s) 160, 360, 591, 972, 1096
BstDSI CCRYGG 1 cut(s) 186
BstH2I RGCGCY 1 cut(s) 1096
BstHHI GCGC 1 cut(s) 1095
BstKTI GATC 6 cut(s) 119, 123, 173, 518, 541, 1069
BstMAI GTCTC 2 cut(s) 497, 668
BstMBI GATC 6 cut(s) 116, 120, 170, 515, 538, 1066
BstMWI GCNNNNNNNGC 2 cut(s) 329, 773
BstNI CCWGG 2 cut(s) 743, 850
BstNSI RCATGY 1 cut(s) 885
BstSCI CCNGG 6 cut(s) 628, 629, 741, 792, 793, 848
BstSLI GKGCMC 1 cut(s) 930
BstV1I GCAGC 3 cut(s) 331, 751, 1066
BstX2I RGATCY 3 cut(s) 170, 515, 1066
BstXI CCANNNNNNTGG 1 cut(s) 828
BstYI RGATCY 3 cut(s) 170, 515, 1066
Bsu15I ATCGAT 1 cut(s) 119
BsuRI GGCC 3 cut(s) 848, 946, 986
BsuTUI ATCGAT 1 cut(s) 119
BtgI CCRYGG 1 cut(s) 186
BtsI GCAGTG 1 cut(s) 915
BtsIMutI CAGTG 1 cut(s) 915
BveI ACCTGC 2 cut(s) 431, 898
Cac8I GCNNGC 1 cut(s) 910
CaiI CAGNNNCTG 1 cut(s) 908
CfoI GCGC 1 cut(s) 1095
Cfr13I GGNCC 3 cut(s) 818, 846, 944
Cfr9I CCCGGG 2 cut(s) 629, 793
ClaI ATCGAT 1 cut(s) 119
Csp6I GTAC 2 cut(s) 959, 1088
CviAII CATG 5 cut(s) 187, 320, 808, 882, 977
CviQI GTAC 2 cut(s) 959, 1088
DdeI CTNAG 5 cut(s) 160, 360, 591, 972, 1096
DpnI GATC 6 cut(s) 118, 122, 172, 517, 540, 1068
DpnII GATC 6 cut(s) 116, 120, 170, 515, 538, 1066
DraI TTTAAA 1 cut(s) 51
Eam1104I CTCTTC 3 cut(s) 32, 187, 237
EarI CTCTTC 3 cut(s) 32, 187, 237
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 778
Eco32I GATATC 1 cut(s) 733
Eco47I GGWCC 1 cut(s) 818
Eco47III AGCGCT 1 cut(s) 1094
Eco57I CTGAAG 1 cut(s) 498
Eco88I CYCGRG 2 cut(s) 629, 793
EcoRII CCWGG 2 cut(s) 741, 848
EcoRV GATATC 1 cut(s) 733
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 778
ErhI CCWWGG 1 cut(s) 186
Esp3I CGTCTC 1 cut(s) 497
FaeI CATG 5 cut(s) 190, 323, 811, 885, 980
FatI CATG 5 cut(s) 186, 319, 807, 881, 976
FauI CCCGC 1 cut(s) 1053
FblI GTMKAC 1 cut(s) 1083
Fnu4HI GCNGC 4 cut(s) 345, 765, 1055, 1058
FriOI GRGCYC 1 cut(s) 778
Fsp4HI GCNGC 4 cut(s) 345, 765, 1055, 1058
FspBI CTAG 4 cut(s) 126, 216, 962, 1035
GlaI GCGC 1 cut(s) 1094
GluI GCNGC 4 cut(s) 345, 765, 1055, 1058
GsaI CCCAGC 1 cut(s) 245
GsuI CTGGAG 1 cut(s) 690
HaeII RGCGCY 1 cut(s) 1096
HaeIII GGCC 3 cut(s) 848, 946, 986
HapII CCGG 2 cut(s) 630, 794
HhaI GCGC 1 cut(s) 1095
Hin1II CATG 5 cut(s) 190, 323, 811, 885, 980
Hin6I GCGC 1 cut(s) 1093
HinP1I GCGC 1 cut(s) 1093
HincII GTYRAC 3 cut(s) 156, 255, 691
HindII GTYRAC 3 cut(s) 156, 255, 691
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 4 cut(s) 43, 650, 899, 1100
HpaII CCGG 2 cut(s) 630, 794
HphI GGTGA 2 cut(s) 458, 879
Hpy166II GTNNAC 4 cut(s) 156, 255, 691, 1084
Hpy188I TCNGA 6 cut(s) 114, 272, 298, 478, 574, 600
Hpy188III TCNNGA 2 cut(s) 496, 903
Hpy8I GTNNAC 4 cut(s) 156, 255, 691, 1084
HpyAV CCTTC 3 cut(s) 468, 1023, 1049
HpyCH4III ACNGT 1 cut(s) 392
HpyCH4IV ACGT 2 cut(s) 490, 1086
HpyCH4V TGCA 3 cut(s) 440, 767, 826
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 773
HpyF3I CTNAG 5 cut(s) 160, 360, 591, 972, 1096
HpySE526I ACGT 2 cut(s) 490, 1086
Hsp92II CATG 5 cut(s) 190, 323, 811, 885, 980
HspAI GCGC 1 cut(s) 1093
Kzo9I GATC 6 cut(s) 116, 120, 170, 515, 538, 1066
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 5 cut(s) 147, 250, 356, 797, 905
Lsp1109I GCAGC 3 cut(s) 331, 751, 1066
LweI GCATC 4 cut(s) 427, 439, 604, 729
MaeI CTAG 4 cut(s) 126, 216, 962, 1035
MaeII ACGT 2 cut(s) 490, 1086
MaeIII GTNAC 3 cut(s) 21, 128, 289
MalI GATC 6 cut(s) 118, 122, 172, 517, 540, 1068
MboI GATC 6 cut(s) 116, 120, 170, 515, 538, 1066
MflI RGATCY 3 cut(s) 170, 515, 1066
MhlI GDGCHC 2 cut(s) 778, 930
MluCI AATT 6 cut(s) 12, 34, 104, 260, 381, 833
MlyI GAGTC 1 cut(s) 1094
MmeI TCCRAC 1 cut(s) 514
MseI TTAA 3 cut(s) 50, 167, 804
MspA1I CMGCKG 1 cut(s) 1057
MspI CCGG 2 cut(s) 630, 794
MspR9I CCNGG 6 cut(s) 630, 631, 743, 794, 795, 850
MvaI CCWGG 2 cut(s) 743, 850
MwoI GCNNNNNNNGC 2 cut(s) 329, 773
NciI CCSGG 4 cut(s) 630, 631, 794, 795
NcoI CCATGG 1 cut(s) 186
NdeII GATC 6 cut(s) 116, 120, 170, 515, 538, 1066
NlaIII CATG 5 cut(s) 190, 323, 811, 885, 980
NlaIV GGNNCC 3 cut(s) 571, 740, 799
NmuCI GTSAC 2 cut(s) 21, 128
NspI RCATGY 1 cut(s) 885
NspV TTCGAA 1 cut(s) 38
PaqCI CACCTGC 2 cut(s) 431, 898
PciSI GCTCTTC 1 cut(s) 237
PfeI GAWTC 3 cut(s) 43, 650, 899
PfoI TCCNGGA 1 cut(s) 741
PkrI GCNGC 4 cut(s) 346, 766, 1056, 1059
PleI GAGTC 1 cut(s) 1094
PpsI GAGTC 1 cut(s) 1094
Psp6I CCWGG 2 cut(s) 741, 848
PspFI CCCAGC 1 cut(s) 241
PspGI CCWGG 2 cut(s) 741, 848
PspN4I GGNNCC 3 cut(s) 571, 740, 799
PspPI GGNCC 3 cut(s) 818, 846, 944
PstNI CAGNNNCTG 1 cut(s) 908
PsuI RGATCY 3 cut(s) 170, 515, 1066
RsaI GTAC 2 cut(s) 960, 1089
RsaNI GTAC 2 cut(s) 959, 1088
SapI GCTCTTC 1 cut(s) 237
SaqAI TTAA 3 cut(s) 50, 167, 804
SatI GCNGC 4 cut(s) 345, 765, 1055, 1058
Sau3AI GATC 6 cut(s) 116, 120, 170, 515, 538, 1066
Sau96I GGNCC 3 cut(s) 818, 846, 944
ScaI AGTACT 1 cut(s) 960
SchI GAGTC 1 cut(s) 1094
ScrFI CCNGG 6 cut(s) 630, 631, 743, 794, 795, 850
SduI GDGCHC 2 cut(s) 778, 930
SfaNI GCATC 4 cut(s) 427, 439, 604, 729
SfuI TTCGAA 1 cut(s) 38
SinI GGWCC 1 cut(s) 818
SmaI CCCGGG 2 cut(s) 631, 795
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
SpeI ACTAGT 1 cut(s) 125
Sse9I AATT 6 cut(s) 12, 34, 104, 260, 381, 833
SsiI CCGC 3 cut(s) 425, 1057, 1060
SspMI CTAG 4 cut(s) 126, 216, 962, 1035
StyD4I CCNGG 6 cut(s) 628, 629, 741, 792, 793, 848
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 1 cut(s) 392
TaiI ACGT 2 cut(s) 493, 1089
TaqI TCGA 2 cut(s) 38, 119
TasI AATT 6 cut(s) 12, 34, 104, 260, 381, 833
TatI WGTACW 1 cut(s) 958
TauI GCSGC 1 cut(s) 1060
TfiI GAWTC 3 cut(s) 43, 650, 899
Tru1I TTAA 3 cut(s) 50, 167, 804
Tru9I TTAA 3 cut(s) 50, 167, 804
TscAI CASTG 1 cut(s) 922
TseFI GTSAC 2 cut(s) 21, 128
TseI GCWGC 3 cut(s) 344, 764, 1054
Tsp45I GTSAC 2 cut(s) 21, 128
TspDTI ATGAA 3 cut(s) 6, 17, 64
TspGWI ACGGA 3 cut(s) 240, 384, 872
TspMI CCCGGG 2 cut(s) 629, 793
TspRI CASTG 1 cut(s) 922
VpaK11BI GGWCC 1 cut(s) 818
XapI RAATTY 2 cut(s) 260, 381
XceI RCATGY 1 cut(s) 885
XmaI CCCGGG 2 cut(s) 629, 793
XmiI GTMKAC 1 cut(s) 1083
XspI CTAG 4 cut(s) 126, 216, 962, 1035
ZrmI AGTACT 1 cut(s) 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.