Rh6CG520800

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0010419
Physical Location & Seq
Forward (+)
67640210 .. 67644909
4700 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG520800.1

Sequence Viewer

Length: 1074 bp
ATGAAAGCAGCACTATCCACTCTGATCAGCTCCAGGACTCTGAGCCCTCCCCCCTCTCCTTCTTCTTCTTTCTCTCGCTCCTTTTGGCTTCAACCAAAGACCCTCTCCAACTCACCCTCCTTCATTACCCTTATCCCCTCCAAGACCCTCACCACCAACACCAACAATTACTCCCGTCTCCGTCTCAATCATCTCAAGTCCCTCACCGCTAATTTCTCGTCGATAATGGCCTCCGCTTACAAGCCCGAAGAAGCCAGGGTCCCACCTGCCCTTCCCTTGCCCACTCCTCCCGTCAACAAGTTTAAGATTGGACTGTGTCAATTGTCGGTGACGGCCAATAAGGAGAGGAACATTGCTCATGCTCGCAAGGCCATCGAGCAGGCTGTCGAAAAGGGTGCTCAGCTGGTTCTCCTACCTGAAATATGGAATGGCCCATATTCAAATGATAGCTTCCCAGTGTTTGCTGAGGATATTGACGCTGGTGGTGATGCATCTCCTTCGACAGCCATGCTTTCTGAAGTTGCTCAGCGCCTGAAAATCACCATTGTTGGTGGATCTATATCAGAGCGTTCTGGTGATCAGCTGTACAATACTTGTTGTGTCTTTGGTACTGATGGAAAGCTGAAAGCTAAACATAGGAAGATACACCTCTTTGATATTGATATCCCTGGGAAGATTACCTTTATTGAATCAAAGACTCTTACAGCAGGGCAAACTCCTACCATTGTGGACACAGAAGTTGGGCGTATTGGCATAGGCATTTGTTATGACATTAGGTTCCAGGAACTAGCTATGATCTATGGAGCAAGAGGTGCTCACTTGATATGCTATCCTGGGGCTTTTAACATGACCACTGGGCCATTGCATTGGGAATTGTTGCAGAGAGCAAGGGCTACAGATAACCAGCTATATGTGGCAACTTGTTCACCTGCTCGAGATGCCGGAGCTGGTTATGTGGCATGGGGCCACTCCACCCTTGTCGGACCAGTATGTTTGACACTAAACTTTTGCTTTGCACATGCTTCCCTTAAATCTTTCCTGGAGCCATTCATTGGCAAGTTGAATTTTTACTAG

Protein Analysis

357

Amino Acids

38.84

Weight (kDa)

9.13

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 102 - 329 5.2e-57 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 274, 937
Acc36I ACCTGC 2 cut(s) 274, 937
AccB7I CCANNNNNTGG 1 cut(s) 1052
AciI CCGC 2 cut(s) 207, 234
AclWI GGATC 1 cut(s) 562
AcoI YGGCCR 1 cut(s) 333
AcsI RAATTY 1 cut(s) 1063
AcuI CTGAAG 1 cut(s) 537
AfaI GTAC 2 cut(s) 587, 610
AfiI CCNNNNNNNGG 1 cut(s) 1052
AgsI TTSAA 4 cut(s) 92, 441, 689, 1063
AjnI CCWGG 6 cut(s) 32, 254, 667, 780, 832, 1038
AluBI AGCT 9 cut(s) 30, 403, 450, 583, 622, 629, 791, 907, 947
AluI AGCT 9 cut(s) 30, 403, 450, 583, 622, 629, 791, 907, 947
Alw21I GWGCWC 2 cut(s) 400, 817
Alw26I GTCTC 2 cut(s) 182, 188
AlwI GGATC 1 cut(s) 562
AlwNI CAGNNNCTG 1 cut(s) 532
Ama87I CYCGRG 1 cut(s) 933
AoxI GGCC 6 cut(s) 228, 333, 369, 430, 857, 964
ApeKI GCWGC 1 cut(s) 8
ApoI RAATTY 1 cut(s) 1063
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 5 cut(s) 259, 431, 857, 964, 983
AsuHPI GGTGA 8 cut(s) 105, 142, 196, 340, 497, 532, 587, 918
AvaI CYCGRG 1 cut(s) 933
AvaII GGWCC 2 cut(s) 259, 983
BanII GRGCYC 1 cut(s) 47
Bbv12I GWGCWC 2 cut(s) 400, 817
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 1 cut(s) 20
BccI CCATC 2 cut(s) 380, 608
BceAI ACGGC 1 cut(s) 348
BcgI CGANNNNNNTGC 8 cut(s) 355, 377, 389, 411, 480, 490, 514, 524
BciT130I CCWGG 6 cut(s) 34, 256, 669, 782, 834, 1040
BclI TGATCA 2 cut(s) 24, 577
BcoDI GTCTC 2 cut(s) 182, 188
BfaI CTAG 2 cut(s) 788, 1072
BfmI CTRYAG 1 cut(s) 894
BfoI RGCGCY 1 cut(s) 532
BfuAI ACCTGC 2 cut(s) 274, 937
BisI GCNGC 1 cut(s) 9
BlpI GCTNAGC 2 cut(s) 399, 525
BlsI GCNGC 1 cut(s) 10
Bme1390I CCNGG 6 cut(s) 34, 256, 669, 782, 834, 1040
Bme18I GGWCC 2 cut(s) 259, 983
BmeT110I CYCGRG 1 cut(s) 933
BmgT120I GGNCC 5 cut(s) 259, 431, 857, 964, 983
BmiI GGNNCC 5 cut(s) 260, 261, 779, 965, 1044
BmrFI CCNGG 6 cut(s) 34, 256, 669, 782, 834, 1040
BmrI ACTGGG 2 cut(s) 449, 864
BmsI GCATC 3 cut(s) 478, 500, 928
BmuI ACTGGG 2 cut(s) 449, 864
BpmI CTGGAG 2 cut(s) 16, 1061
Bpu10I CCTNAGC 1 cut(s) 465
Bpu1102I GCTNAGC 2 cut(s) 399, 525
BpuEI CTTGAG 1 cut(s) 179
BsaBI GATNNNNATC 1 cut(s) 559
BsaJI CCNNGG 4 cut(s) 255, 667, 668, 833
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 155, 185
Bsc4I CCNNNNNNNGG 1 cut(s) 1052
Bse1I ACTGG 3 cut(s) 455, 859, 986
Bse3DI GCAATG 2 cut(s) 351, 860
Bse8I GATNNNNATC 1 cut(s) 559
BseBI CCWGG 6 cut(s) 34, 256, 669, 782, 834, 1040
BseDI CCNNGG 4 cut(s) 255, 667, 668, 833
BseJI GATNNNNATC 1 cut(s) 559
BseLI CCNNNNNNNGG 1 cut(s) 1052
BseMI GCAATG 2 cut(s) 351, 860
BseMII CTCAG 4 cut(s) 32, 413, 456, 539
BseNI ACTGG 3 cut(s) 455, 859, 986
BseRI GAGGAG 1 cut(s) 276
BseXI GCAGC 1 cut(s) 20
BshFI GGCC 6 cut(s) 230, 335, 371, 432, 859, 966
BsiHKAI GWGCWC 2 cut(s) 400, 817
BsiHKCI CYCGRG 1 cut(s) 933
BsiSI CCGG 1 cut(s) 942
BslFI GGGAC 2 cut(s) 184, 245
BslI CCNNNNNNNGG 1 cut(s) 1052
BsmAI GTCTC 2 cut(s) 182, 188
BsmBI CGTCTC 2 cut(s) 182, 188
BsmFI GGGAC 2 cut(s) 184, 245
BsnI GGCC 6 cut(s) 230, 335, 371, 432, 859, 966
BsoBI CYCGRG 1 cut(s) 933
Bsp1286I GDGCHC 3 cut(s) 47, 400, 817
Bsp1407I TGTACA 1 cut(s) 585
Bsp143I GATC 4 cut(s) 24, 554, 577, 795
Bsp1720I GCTNAGC 2 cut(s) 399, 525
BspACI CCGC 2 cut(s) 207, 234
BspANI GGCC 6 cut(s) 230, 335, 371, 432, 859, 966
BspCNI CTCAG 4 cut(s) 33, 412, 457, 538
BspLI GGNNCC 5 cut(s) 260, 261, 779, 965, 1044
BspMI ACCTGC 2 cut(s) 274, 937
BspPI GGATC 1 cut(s) 562
BsrDI GCAATG 2 cut(s) 351, 860
BsrGI TGTACA 1 cut(s) 585
BsrI ACTGG 3 cut(s) 455, 859, 986
BssECI CCNNGG 4 cut(s) 255, 667, 668, 833
BssMI GATC 4 cut(s) 24, 554, 577, 795
Bst2UI CCWGG 6 cut(s) 34, 256, 669, 782, 834, 1040
Bst4CI ACNGT 1 cut(s) 315
BstAPI GCANNNNNTGC 1 cut(s) 812
BstAUI TGTACA 1 cut(s) 585
BstC8I GCNNGC 2 cut(s) 364, 381
BstDEI CTNAG 4 cut(s) 41, 399, 465, 525
BstH2I RGCGCY 1 cut(s) 532
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 4 cut(s) 27, 557, 580, 798
BstMAI GTCTC 2 cut(s) 182, 188
BstMBI GATC 4 cut(s) 24, 554, 577, 795
BstMWI GCNNNNNNNGC 3 cut(s) 368, 812, 938
BstNI CCWGG 6 cut(s) 34, 256, 669, 782, 834, 1040
BstNSI RCATGY 1 cut(s) 1022
BstSCI CCNGG 6 cut(s) 32, 254, 667, 780, 832, 1038
BstSFI CTRYAG 1 cut(s) 894
BstV1I GCAGC 1 cut(s) 20
BstX2I RGATCY 1 cut(s) 554
BstXI CCANNNNNNTGG 1 cut(s) 867
BstYI RGATCY 1 cut(s) 554
BsuRI GGCC 6 cut(s) 230, 335, 371, 432, 859, 966
BtsIMutI CAGTG 2 cut(s) 462, 852
BveI ACCTGC 2 cut(s) 274, 937
Cac8I GCNNGC 2 cut(s) 364, 381
CaiI CAGNNNCTG 1 cut(s) 532
CfoI GCGC 1 cut(s) 531
Cfr13I GGNCC 5 cut(s) 259, 431, 857, 964, 983
CseI GACGC 1 cut(s) 485
Csp6I GTAC 2 cut(s) 586, 609
CviAII CATG 5 cut(s) 359, 508, 847, 960, 1019
CviQI GTAC 2 cut(s) 586, 609
DdeI CTNAG 4 cut(s) 41, 399, 465, 525
DpnI GATC 4 cut(s) 26, 556, 579, 797
DpnII GATC 4 cut(s) 24, 554, 577, 795
EaeI YGGCCR 1 cut(s) 333
Eco24I GRGCYC 1 cut(s) 47
Eco32I GATATC 1 cut(s) 664
Eco47I GGWCC 2 cut(s) 259, 983
Eco57I CTGAAG 1 cut(s) 537
Eco88I CYCGRG 1 cut(s) 933
EcoO109I RGGNCCY 1 cut(s) 259
EcoRII CCWGG 6 cut(s) 32, 254, 667, 780, 832, 1038
EcoRV GATATC 1 cut(s) 664
EcoT22I ATGCAT 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 47
Esp3I CGTCTC 2 cut(s) 182, 188
FaeI CATG 5 cut(s) 362, 511, 850, 963, 1022
FalI AAGNNNNNCTT 2 cut(s) 665, 697
FaqI GGGAC 2 cut(s) 184, 245
FatI CATG 5 cut(s) 358, 507, 846, 959, 1018
FbaI TGATCA 2 cut(s) 24, 577
Fnu4HI GCNGC 1 cut(s) 9
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 1 cut(s) 9
FspBI CTAG 2 cut(s) 788, 1072
GlaI GCGC 1 cut(s) 530
GluI GCNGC 1 cut(s) 9
GsuI CTGGAG 2 cut(s) 16, 1061
HaeII RGCGCY 1 cut(s) 532
HaeIII GGCC 6 cut(s) 230, 335, 371, 432, 859, 966
HapII CCGG 1 cut(s) 942
HgaI GACGC 1 cut(s) 485
HhaI GCGC 1 cut(s) 531
Hin1II CATG 5 cut(s) 362, 511, 850, 963, 1022
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 3 cut(s) 37, 689, 697
HpaII CCGG 1 cut(s) 942
HphI GGTGA 8 cut(s) 105, 142, 196, 340, 497, 532, 587, 918
Hpy166II GTNNAC 3 cut(s) 295, 730, 926
Hpy188I TCNGA 5 cut(s) 24, 42, 517, 565, 983
Hpy188III TCNNGA 1 cut(s) 935
Hpy8I GTNNAC 3 cut(s) 295, 730, 926
Hpy99I CGWCG 1 cut(s) 223
HpyAV CCTTC 4 cut(s) 69, 130, 281, 507
HpyCH4III ACNGT 1 cut(s) 315
HpyCH4V TGCA 4 cut(s) 491, 865, 880, 1016
HpyF10VI GCNNNNNNNGC 3 cut(s) 368, 812, 938
HpyF3I CTNAG 4 cut(s) 41, 399, 465, 525
Hsp92II CATG 5 cut(s) 362, 511, 850, 963, 1022
HspAI GCGC 1 cut(s) 529
KflI GGGWCCC 1 cut(s) 259
Ksp22I TGATCA 2 cut(s) 24, 577
Kzo9I GATC 4 cut(s) 24, 554, 577, 795
LmnI GCTCC 5 cut(s) 35, 83, 803, 944, 1042
Lsp1109I GCAGC 1 cut(s) 20
LweI GCATC 3 cut(s) 478, 500, 928
MaeI CTAG 2 cut(s) 788, 1072
MaeIII GTNAC 1 cut(s) 328
MalI GATC 4 cut(s) 26, 556, 579, 797
MboI GATC 4 cut(s) 24, 554, 577, 795
MboII GAAGA 5 cut(s) 54, 57, 260, 652, 685
MfeI CAATTG 1 cut(s) 320
MflI RGATCY 1 cut(s) 554
MhlI GDGCHC 3 cut(s) 47, 400, 817
MluCI AATT 5 cut(s) 166, 211, 320, 872, 1063
MlyI GAGTC 2 cut(s) 31, 691
MmeI TCCRAC 2 cut(s) 132, 961
Mph1103I ATGCAT 1 cut(s) 493
MseI TTAA 3 cut(s) 303, 843, 1029
MspA1I CMGCKG 2 cut(s) 403, 583
MspI CCGG 1 cut(s) 942
MspR9I CCNGG 6 cut(s) 34, 256, 669, 782, 834, 1040
MunI CAATTG 1 cut(s) 320
MvaI CCWGG 6 cut(s) 34, 256, 669, 782, 834, 1040
MwoI GCNNNNNNNGC 3 cut(s) 368, 812, 938
NdeII GATC 4 cut(s) 24, 554, 577, 795
NlaIII CATG 5 cut(s) 362, 511, 850, 963, 1022
NlaIV GGNNCC 5 cut(s) 260, 261, 779, 965, 1044
NmuCI GTSAC 1 cut(s) 328
NsiI ATGCAT 1 cut(s) 493
NspI RCATGY 1 cut(s) 1022
PaeR7I CTCGAG 1 cut(s) 933
PaqCI CACCTGC 2 cut(s) 274, 937
PasI CCCWGGG 1 cut(s) 668
PfeI GAWTC 1 cut(s) 689
PflFI GACNNNGTC 1 cut(s) 315
PflMI CCANNNNNTGG 1 cut(s) 1052
PfoI TCCNGGA 3 cut(s) 32, 780, 1038
PkrI GCNGC 1 cut(s) 10
PleI GAGTC 2 cut(s) 31, 691
PpsI GAGTC 2 cut(s) 31, 691
PpuMI RGGWCCY 1 cut(s) 259
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 6 cut(s) 32, 254, 667, 780, 832, 1038
PspGI CCWGG 6 cut(s) 32, 254, 667, 780, 832, 1038
PspN4I GGNNCC 5 cut(s) 260, 261, 779, 965, 1044
PspPI GGNCC 5 cut(s) 259, 431, 857, 964, 983
PspPPI RGGWCCY 1 cut(s) 259
PstNI CAGNNNCTG 1 cut(s) 532
PsuI RGATCY 1 cut(s) 554
PsyI GACNNNGTC 1 cut(s) 315
PvuII CAGCTG 2 cut(s) 403, 583
RsaI GTAC 2 cut(s) 587, 610
RsaNI GTAC 2 cut(s) 586, 609
SaqAI TTAA 3 cut(s) 303, 843, 1029
SatI GCNGC 1 cut(s) 9
Sau3AI GATC 4 cut(s) 24, 554, 577, 795
Sau96I GGNCC 5 cut(s) 259, 431, 857, 964, 983
SchI GAGTC 2 cut(s) 31, 691
ScrFI CCNGG 6 cut(s) 34, 256, 669, 782, 834, 1040
SduI GDGCHC 3 cut(s) 47, 400, 817
SfaNI GCATC 3 cut(s) 478, 500, 928
SfcI CTRYAG 1 cut(s) 894
Sfr274I CTCGAG 1 cut(s) 933
SinI GGWCC 2 cut(s) 259, 983
SlaI CTCGAG 1 cut(s) 933
SmlI CTYRAG 2 cut(s) 194, 933
SmoI CTYRAG 2 cut(s) 194, 933
Sse9I AATT 5 cut(s) 166, 211, 320, 872, 1063
SsiI CCGC 2 cut(s) 207, 234
SspMI CTAG 2 cut(s) 788, 1072
StyD4I CCNGG 6 cut(s) 32, 254, 667, 780, 832, 1038
TaaI ACNGT 1 cut(s) 315
TaqI TCGA 5 cut(s) 221, 375, 387, 500, 934
TasI AATT 5 cut(s) 166, 211, 320, 872, 1063
TatI WGTACW 1 cut(s) 585
TfiI GAWTC 1 cut(s) 689
Tru1I TTAA 3 cut(s) 303, 843, 1029
Tru9I TTAA 3 cut(s) 303, 843, 1029
TscAI CASTG 2 cut(s) 462, 859
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 1 cut(s) 8
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 3 cut(s) 17, 112, 1039
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 2 cut(s) 462, 859
Tth111I GACNNNGTC 1 cut(s) 315
Van91I CCANNNNNTGG 1 cut(s) 1052
VpaK11BI GGWCC 2 cut(s) 259, 983
XapI RAATTY 1 cut(s) 1063
XceI RCATGY 1 cut(s) 1022
XhoI CTCGAG 1 cut(s) 933
XspI CTAG 2 cut(s) 788, 1072
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.