FvH4_2g31270

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010419
Physical Location & Seq
Reverse (-)
24016944 .. 24020689
3746 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g31270.t1

Sequence Viewer

Length: 1122 bp
ATGAAAGCAGCACTGTCCATGCTAATCAGCTCCAGGAACCTGAGCCCTCACCCCTCTTCTTCTCTCTCTCGCTCCACTTGGCTTCGCCCAAAGTCCCTCCCCAACTCACCCTCCATTATTCCCCTTATACCCTCCAAGTCCCTCTCCCGTCTCCGCCTCAATCACCGCAAGTCCCACACCGCTCATTTCTCGTCGATAATGGCCTCCTCTTACAAGCCCGAAGAAGCCAGGGTCCCACCTGCCCTTCCCTTACCCGCTCCTCCCGTCAACAAGTTCAAGATTGGGCTGTGCCAGCTGTCGGTGACGGCCGATAAGGAGAGGAACATTGCTCATGCTCGCAAGGCCATCGAGCAGGCTGCCGCACAGGGTGCTCAGCTGGTTGTCTTACCTGAAATATGGAATGGGCCGTATTCAAATGATAGTTTTCCAGTGTTTGCGGAGGATATTGATGCCGGCGGTGATGCATCTCCTTCGACAGCAATGCTTGCGGAAGTTGCTCAGCGACTGAAAATCACCATTGTTGGTGGGTCTATATCGGAGCGTTCTGGTGATCAGCTGTACAATACTTGTTGTGTGTTTGGTACTGATGGAAAGCTCAAAGCTAAACATAGGAAGATACACCTCTTTGATATTGATATCCCTGGGAAGATTACCTTTATTGAATCAAAGACTCTGACAGCAGGGCAAACTCCTACCATTGTGGACACAGAAGTTGGGCGTATTGGCATAGGAATTTGTTATGACATTAGGTTCCAGGAACTAGCTATGATCTATGGAGCAAGAGGTGCTCACTTGATATGCTATCCTGGGGCTTTTAACATGACCACTGGACCATTGCATTGGGAATTGTTGCAGAGAGCAAGGGCTACAGATAACCAGCTATATGTGGCAACTTGTTCACCTGCCCGAGATGCCGGAGCTGGTTATGTGGCATGGGGACACTCCACCCTTGTCGGACCATTTGGAGAAGTGCTGGCGACTACAGAACATGAAGAGGCAATAATCATGGCTGAGGTTGATTATTCACTCCTTGAGCTTAGAAGGACAAATCTCCCTCTACTGAACCAGCGGCGAGGAGATCTTTACCAGTTGGTAGATGTCCAGAGGTTGAATTCTCAGTGA

Protein Analysis

374

Amino Acids

40.73

Weight (kDa)

8.82

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 93 - 345 2.4e-64 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 247, 910
Acc36I ACCTGC 2 cut(s) 247, 910
AccBSI CCGCTC 2 cut(s) 182, 257
AciI CCGC 9 cut(s) 154, 166, 180, 255, 360, 437, 456, 488, 1069
AcoI YGGCCR 1 cut(s) 306
AcsI RAATTY 2 cut(s) 732, 1111
AdeI CACNNNGTG 1 cut(s) 368
AfaI GTAC 2 cut(s) 560, 583
AfiI CCNNNNNNNGG 1 cut(s) 298
AgsI TTSAA 4 cut(s) 277, 414, 662, 1111
AjnI CCWGG 5 cut(s) 32, 227, 640, 753, 805
Alw21I GWGCWC 2 cut(s) 373, 790
Alw26I GTCTC 1 cut(s) 155
AlwNI CAGNNNCTG 1 cut(s) 505
Ama87I CYCGRG 1 cut(s) 906
AoxI GGCC 4 cut(s) 201, 306, 342, 404
ApeKI GCWGC 2 cut(s) 8, 356
ApoI RAATTY 2 cut(s) 732, 1111
AspS9I GGNCC 4 cut(s) 232, 404, 830, 956
AsuHPI GGTGA 8 cut(s) 41, 99, 155, 313, 470, 505, 560, 891
AvaI CYCGRG 1 cut(s) 906
AvaII GGWCC 3 cut(s) 232, 830, 956
BanII GRGCYC 1 cut(s) 47
Bbv12I GWGCWC 2 cut(s) 373, 790
BbvCI CCTCAGC 1 cut(s) 1011
BbvI GCAGC 2 cut(s) 20, 343
BccI CCATC 2 cut(s) 353, 581
BceAI ACGGC 2 cut(s) 321, 391
BcgI CGANNNNNNTGC 8 cut(s) 328, 338, 362, 372, 453, 463, 487, 497
BciT130I CCWGG 5 cut(s) 34, 229, 642, 755, 807
BclI TGATCA 1 cut(s) 550
BcoDI GTCTC 1 cut(s) 155
BfaI CTAG 1 cut(s) 761
BfmI CTRYAG 2 cut(s) 867, 981
BfuAI ACCTGC 2 cut(s) 247, 910
BglII AGATCT 1 cut(s) 1078
BisI GCNGC 4 cut(s) 9, 357, 360, 1070
BlpI GCTNAGC 2 cut(s) 372, 498
BlsI GCNGC 4 cut(s) 10, 358, 361, 1071
Bme1390I CCNGG 5 cut(s) 34, 229, 642, 755, 807
Bme18I GGWCC 3 cut(s) 232, 830, 956
BmeT110I CYCGRG 1 cut(s) 906
BmgT120I GGNCC 4 cut(s) 232, 404, 830, 956
BmiI GGNNCC 4 cut(s) 38, 233, 234, 752
BmrFI CCNGG 5 cut(s) 34, 229, 642, 755, 807
BmsI GCATC 4 cut(s) 439, 451, 473, 901
BpmI CTGGAG 1 cut(s) 16
Bpu10I CCTNAGC 2 cut(s) 41, 1011
Bpu1102I GCTNAGC 2 cut(s) 372, 498
BpuEI CTTGAG 1 cut(s) 1052
BsaJI CCNNGG 4 cut(s) 228, 640, 641, 806
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse118I RCCGGY 1 cut(s) 452
Bse1I ACTGG 3 cut(s) 428, 832, 1087
Bse3DI GCAATG 3 cut(s) 324, 486, 833
BseBI CCWGG 5 cut(s) 34, 229, 642, 755, 807
BseDI CCNNGG 4 cut(s) 228, 640, 641, 806
BseLI CCNNNNNNNGG 1 cut(s) 298
BseMI GCAATG 3 cut(s) 324, 486, 833
BseMII CTCAG 4 cut(s) 32, 386, 512, 1002
BseNI ACTGG 3 cut(s) 428, 832, 1087
BseRI GAGGAG 3 cut(s) 196, 249, 1089
BseX3I CGGCCG 1 cut(s) 306
BseXI GCAGC 2 cut(s) 20, 343
Bsh1285I CGRYCG 1 cut(s) 309
BshFI GGCC 4 cut(s) 203, 308, 344, 406
BsiEI CGRYCG 1 cut(s) 309
BsiHKAI GWGCWC 2 cut(s) 373, 790
BsiHKCI CYCGRG 1 cut(s) 906
BsiSI CCGG 2 cut(s) 453, 915
BslFI GGGAC 5 cut(s) 79, 124, 157, 218, 951
BslI CCNNNNNNNGG 1 cut(s) 298
BsmAI GTCTC 1 cut(s) 155
BsmBI CGTCTC 1 cut(s) 155
BsmFI GGGAC 5 cut(s) 79, 124, 157, 218, 951
BsnI GGCC 4 cut(s) 203, 308, 344, 406
BsoBI CYCGRG 1 cut(s) 906
Bsp1286I GDGCHC 3 cut(s) 47, 373, 790
Bsp1407I TGTACA 1 cut(s) 558
Bsp143I GATC 3 cut(s) 550, 768, 1078
Bsp1720I GCTNAGC 2 cut(s) 372, 498
BspACI CCGC 9 cut(s) 154, 166, 180, 255, 360, 437, 456, 488, 1069
BspANI GGCC 4 cut(s) 203, 308, 344, 406
BspCNI CTCAG 4 cut(s) 33, 385, 511, 1003
BspLI GGNNCC 4 cut(s) 38, 233, 234, 752
BspMI ACCTGC 2 cut(s) 247, 910
BsrBI CCGCTC 2 cut(s) 182, 257
BsrDI GCAATG 3 cut(s) 324, 486, 833
BsrFI RCCGGY 1 cut(s) 452
BsrGI TGTACA 1 cut(s) 558
BsrI ACTGG 3 cut(s) 428, 832, 1087
BssAI RCCGGY 1 cut(s) 452
BssECI CCNNGG 4 cut(s) 228, 640, 641, 806
BssMI GATC 3 cut(s) 550, 768, 1078
Bst2UI CCWGG 5 cut(s) 34, 229, 642, 755, 807
Bst4CI ACNGT 1 cut(s) 15
Bst6I CTCTTC 2 cut(s) 61, 987
BstAPI GCANNNNNTGC 3 cut(s) 368, 485, 785
BstAUI TGTACA 1 cut(s) 558
BstC8I GCNNGC 6 cut(s) 293, 337, 354, 454, 486, 975
BstDEI CTNAG 6 cut(s) 41, 372, 498, 1011, 1037, 1116
BstKTI GATC 3 cut(s) 553, 771, 1081
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 3 cut(s) 550, 768, 1078
BstMCI CGRYCG 1 cut(s) 309
BstMWI GCNNNNNNNGC 7 cut(s) 292, 341, 368, 485, 494, 785, 911
BstNI CCWGG 5 cut(s) 34, 229, 642, 755, 807
BstSCI CCNGG 5 cut(s) 32, 227, 640, 753, 805
BstSFI CTRYAG 2 cut(s) 867, 981
BstV1I GCAGC 2 cut(s) 20, 343
BstX2I RGATCY 1 cut(s) 1078
BstXI CCANNNNNNTGG 1 cut(s) 840
BstYI RGATCY 1 cut(s) 1078
BstZI CGGCCG 1 cut(s) 306
BsuRI GGCC 4 cut(s) 203, 308, 344, 406
BtsIMutI CAGTG 3 cut(s) 11, 435, 825
BveI ACCTGC 2 cut(s) 247, 910
Cac8I GCNNGC 6 cut(s) 293, 337, 354, 454, 486, 975
CaiI CAGNNNCTG 1 cut(s) 505
Cfr10I RCCGGY 1 cut(s) 452
Cfr13I GGNCC 4 cut(s) 232, 404, 830, 956
Csp6I GTAC 2 cut(s) 559, 582
CviAII CATG 6 cut(s) 19, 332, 820, 933, 989, 1006
CviQI GTAC 2 cut(s) 559, 582
DdeI CTNAG 6 cut(s) 41, 372, 498, 1011, 1037, 1116
DpnI GATC 3 cut(s) 552, 770, 1080
DpnII GATC 3 cut(s) 550, 768, 1078
DraIII CACNNNGTG 1 cut(s) 368
EaeI YGGCCR 1 cut(s) 306
EagI CGGCCG 1 cut(s) 306
Eam1104I CTCTTC 2 cut(s) 61, 987
EarI CTCTTC 2 cut(s) 61, 987
EciI GGCGGA 1 cut(s) 143
EclXI CGGCCG 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 47
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 3 cut(s) 232, 830, 956
Eco52I CGGCCG 1 cut(s) 306
Eco88I CYCGRG 1 cut(s) 906
EcoO109I RGGNCCY 1 cut(s) 232
EcoRI GAATTC 1 cut(s) 1111
EcoRII CCWGG 5 cut(s) 32, 227, 640, 753, 805
EcoRV GATATC 1 cut(s) 637
EcoT22I ATGCAT 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 47
Esp3I CGTCTC 1 cut(s) 155
FaeI CATG 6 cut(s) 22, 335, 823, 936, 992, 1009
FalI AAGNNNNNCTT 2 cut(s) 638, 670
FaqI GGGAC 5 cut(s) 79, 124, 157, 218, 951
FatI CATG 6 cut(s) 18, 331, 819, 932, 988, 1005
FauI CCCGC 1 cut(s) 262
FbaI TGATCA 1 cut(s) 550
Fnu4HI GCNGC 4 cut(s) 9, 357, 360, 1070
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 4 cut(s) 9, 357, 360, 1070
FspBI CTAG 1 cut(s) 761
GluI GCNGC 4 cut(s) 9, 357, 360, 1070
GsuI CTGGAG 1 cut(s) 16
HaeIII GGCC 4 cut(s) 203, 308, 344, 406
HapII CCGG 2 cut(s) 453, 915
Hin1II CATG 6 cut(s) 22, 335, 823, 936, 992, 1009
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 2 cut(s) 662, 670
HpaII CCGG 2 cut(s) 453, 915
HphI GGTGA 8 cut(s) 41, 99, 155, 313, 470, 505, 560, 891
Hpy166II GTNNAC 3 cut(s) 268, 703, 899
Hpy188I TCNGA 3 cut(s) 538, 675, 956
Hpy188III TCNNGA 2 cut(s) 277, 1102
Hpy8I GTNNAC 3 cut(s) 268, 703, 899
Hpy99I CGWCG 1 cut(s) 196
HpyAV CCTTC 3 cut(s) 254, 480, 1035
HpyCH4III ACNGT 1 cut(s) 15
HpyCH4V TGCA 3 cut(s) 464, 838, 853
HpyF10VI GCNNNNNNNGC 7 cut(s) 292, 341, 368, 485, 494, 785, 911
HpyF3I CTNAG 6 cut(s) 41, 372, 498, 1011, 1037, 1116
Hsp92II CATG 6 cut(s) 22, 335, 823, 936, 992, 1009
KflI GGGWCCC 1 cut(s) 232
KroI GCCGGC 1 cut(s) 452
KroNI GCCGGC 1 cut(s) 454
Ksp22I TGATCA 1 cut(s) 550
Kzo9I GATC 3 cut(s) 550, 768, 1078
LmnI GCTCC 6 cut(s) 35, 77, 262, 538, 776, 917
Lsp1109I GCAGC 2 cut(s) 20, 343
LweI GCATC 4 cut(s) 439, 451, 473, 901
MaeI CTAG 1 cut(s) 761
MaeIII GTNAC 1 cut(s) 301
MalI GATC 3 cut(s) 552, 770, 1080
MbiI CCGCTC 2 cut(s) 182, 257
MboI GATC 3 cut(s) 550, 768, 1078
MboII GAAGA 6 cut(s) 48, 51, 233, 625, 658, 1004
MflI RGATCY 1 cut(s) 1078
MhlI GDGCHC 3 cut(s) 47, 373, 790
MluCI AATT 3 cut(s) 732, 845, 1111
MlyI GAGTC 1 cut(s) 664
MmeI TCCRAC 1 cut(s) 934
Mph1103I ATGCAT 1 cut(s) 466
MroNI GCCGGC 1 cut(s) 452
MseI TTAA 1 cut(s) 816
MspA1I CMGCKG 4 cut(s) 295, 376, 556, 1069
MspI CCGG 2 cut(s) 453, 915
MspR9I CCNGG 5 cut(s) 34, 229, 642, 755, 807
MvaI CCWGG 5 cut(s) 34, 229, 642, 755, 807
MwoI GCNNNNNNNGC 7 cut(s) 292, 341, 368, 485, 494, 785, 911
NaeI GCCGGC 1 cut(s) 454
NdeII GATC 3 cut(s) 550, 768, 1078
NgoMIV GCCGGC 1 cut(s) 452
NlaIII CATG 6 cut(s) 22, 335, 823, 936, 992, 1009
NlaIV GGNNCC 4 cut(s) 38, 233, 234, 752
NmuCI GTSAC 1 cut(s) 301
NsiI ATGCAT 1 cut(s) 466
PaqCI CACCTGC 2 cut(s) 247, 910
PasI CCCWGGG 1 cut(s) 641
PdiI GCCGGC 1 cut(s) 454
PfeI GAWTC 1 cut(s) 662
PfoI TCCNGGA 2 cut(s) 32, 753
PkrI GCNGC 4 cut(s) 10, 358, 361, 1071
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
PpuMI RGGWCCY 1 cut(s) 232
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 5 cut(s) 32, 227, 640, 753, 805
PspGI CCWGG 5 cut(s) 32, 227, 640, 753, 805
PspN4I GGNNCC 4 cut(s) 38, 233, 234, 752
PspPI GGNCC 4 cut(s) 232, 404, 830, 956
PspPPI RGGWCCY 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 505
PsuI RGATCY 1 cut(s) 1078
PvuII CAGCTG 3 cut(s) 295, 376, 556
RsaI GTAC 2 cut(s) 560, 583
RsaNI GTAC 2 cut(s) 559, 582
SaqAI TTAA 1 cut(s) 816
SatI GCNGC 4 cut(s) 9, 357, 360, 1070
Sau3AI GATC 3 cut(s) 550, 768, 1078
Sau96I GGNCC 4 cut(s) 232, 404, 830, 956
SchI GAGTC 1 cut(s) 664
ScrFI CCNGG 5 cut(s) 34, 229, 642, 755, 807
SduI GDGCHC 3 cut(s) 47, 373, 790
SfaNI GCATC 4 cut(s) 439, 451, 473, 901
SfcI CTRYAG 2 cut(s) 867, 981
SinI GGWCC 3 cut(s) 232, 830, 956
SmlI CTYRAG 1 cut(s) 1031
SmoI CTYRAG 1 cut(s) 1031
Sse9I AATT 3 cut(s) 732, 845, 1111
SsiI CCGC 9 cut(s) 154, 166, 180, 255, 360, 437, 456, 488, 1069
SspMI CTAG 1 cut(s) 761
StyD4I CCNGG 5 cut(s) 32, 227, 640, 753, 805
TaaI ACNGT 1 cut(s) 15
TaqI TCGA 3 cut(s) 194, 348, 473
TasI AATT 3 cut(s) 732, 845, 1111
TatI WGTACW 1 cut(s) 558
TauI GCSGC 2 cut(s) 362, 1072
TfiI GAWTC 1 cut(s) 662
Tru1I TTAA 1 cut(s) 816
Tru9I TTAA 1 cut(s) 816
TscAI CASTG 3 cut(s) 18, 435, 832
TseFI GTSAC 1 cut(s) 301
TseI GCWGC 2 cut(s) 8, 356
Tsp45I GTSAC 1 cut(s) 301
TspDTI ATGAA 2 cut(s) 17, 1005
TspRI CASTG 3 cut(s) 18, 435, 832
VpaK11BI GGWCC 3 cut(s) 232, 830, 956
XapI RAATTY 2 cut(s) 732, 1111
XspI CTAG 1 cut(s) 761
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.