Rroxscaffold_7G00157530

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010419
Physical Location & Seq
Reverse (-)
1349872 .. 1354647
4776 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00157530.1

Sequence Viewer

Length: 924 bp
ATGGCCTCCGCTTACAAGCCCGAAGAAGCCAGGGTCCCACCTGCCCTTCCCTTGTCCACTCCTCCGGTCAACAAGTTTAAGATTGGACTGTGCCAATTGTCGGTGACGGCCGATAAGGAGAGGAACATTGCTCATGCTCGCAAGGCCATCGAGCAGGCTGCCGCACAGGGTGCTCAGCTGGTTCTCCTACCTGAAATATGGAATGGCCCATATTCAAATGATAGCTTCCCAGTGTTTGCTGAGGATATTGACGCTGGTGGTGATGCATCTCCTTCGACAGCCATGCTTTCTGAAGTTGCTCAGCGCCTGAAAATCACCATTGTTGGTGGATCTATATCAGAGCGTTCTGGTGATCAGCTGTACAATACTTGTTGTGTCTTTGGTACTGATGGAAAGCTGAAAGCTAAACATAGGAAGATACACCTCTTTGATATTGATATCCCTGGGAAGATTACCTTTATTGAATCAAAGACTCTTACAGCAGGGCAAACTCCTACCATTGTGGACACTGAAGTTGGGCGTATTGGCATAGGCATTTGTTATGACATTAGGTTCCAGGAACTAGCTATGATCTATGGAGCAAGAGGTGCTCACTTGATATGCTATCCTGGGGCTTTTAACATGACCACTGGGCCATTGCATTGGGAATTGTTGCAGAGAGCAAGGGCTACAGATAACCAGCTATATGTGGCAACTTGTTCACCTGCTCGAGATGCCGGAGCTGGTTATGTGGCATGGGGCCACTCCACCCTTGTTGGACCATTTGGAGAAGTGCTGGCAACTACAGAACATGAAGAGGCAATAATCATAGCTGAGGTTGATTATTCACTCCTTGAGCTTAGAAGAACAAATCTCCCTTTACTGAAGCAGCGGCGAGGTGATCTTTACCAGTTGGTAGATGTTCAGAGGTTGAATTCTAAATGA

Protein Analysis

307

Amino Acids

33.46

Weight (kDa)

6.18

Isoelectric Point (pI)

32.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 27 - 279 1.2e-64 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 49, 712
Acc36I ACCTGC 2 cut(s) 49, 712
AciI CCGC 3 cut(s) 9, 162, 871
AclWI GGATC 1 cut(s) 337
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 1 cut(s) 913
AcuI CTGAAG 3 cut(s) 312, 531, 884
AdeI CACNNNGTG 1 cut(s) 170
AfaI GTAC 2 cut(s) 362, 385
AfiI CCNNNNNNNGG 1 cut(s) 100
AgsI TTSAA 3 cut(s) 216, 464, 913
AjnI CCWGG 4 cut(s) 29, 442, 555, 607
Alw21I GWGCWC 2 cut(s) 175, 592
AlwI GGATC 1 cut(s) 337
AlwNI CAGNNNCTG 1 cut(s) 307
Ama87I CYCGRG 1 cut(s) 708
AoxI GGCC 6 cut(s) 3, 108, 144, 205, 632, 739
ApeKI GCWGC 2 cut(s) 158, 868
ApoI RAATTY 1 cut(s) 913
AspLEI GCGC 1 cut(s) 306
AspS9I GGNCC 5 cut(s) 34, 206, 632, 739, 758
AsuHPI GGTGA 6 cut(s) 115, 272, 307, 362, 693, 890
AvaI CYCGRG 1 cut(s) 708
AvaII GGWCC 2 cut(s) 34, 758
Bbv12I GWGCWC 2 cut(s) 175, 592
BbvCI CCTCAGC 2 cut(s) 240, 813
BbvI GCAGC 2 cut(s) 145, 880
BccI CCATC 2 cut(s) 155, 383
BceAI ACGGC 1 cut(s) 123
BcgI CGANNNNNNTGC 8 cut(s) 130, 140, 164, 174, 255, 265, 289, 299
BciT130I CCWGG 4 cut(s) 31, 444, 557, 609
BclI TGATCA 1 cut(s) 352
BfaI CTAG 1 cut(s) 563
BfmI CTRYAG 2 cut(s) 669, 783
BfoI RGCGCY 1 cut(s) 307
BfuAI ACCTGC 2 cut(s) 49, 712
BisI GCNGC 4 cut(s) 159, 162, 869, 872
BlpI GCTNAGC 2 cut(s) 174, 300
BlsI GCNGC 4 cut(s) 160, 163, 870, 873
Bme1390I CCNGG 4 cut(s) 31, 444, 557, 609
Bme18I GGWCC 2 cut(s) 34, 758
BmeT110I CYCGRG 1 cut(s) 708
BmgT120I GGNCC 5 cut(s) 34, 206, 632, 739, 758
BmiI GGNNCC 4 cut(s) 35, 36, 554, 740
BmrFI CCNGG 4 cut(s) 31, 444, 557, 609
BmrI ACTGGG 2 cut(s) 224, 639
BmsI GCATC 3 cut(s) 253, 275, 703
BmuI ACTGGG 2 cut(s) 224, 639
Bpu10I CCTNAGC 2 cut(s) 240, 813
Bpu1102I GCTNAGC 2 cut(s) 174, 300
BpuEI CTTGAG 1 cut(s) 854
BsaBI GATNNNNATC 1 cut(s) 334
BsaJI CCNNGG 4 cut(s) 30, 442, 443, 608
BsaWI WCCGGW 1 cut(s) 64
Bsc4I CCNNNNNNNGG 1 cut(s) 100
Bse1I ACTGG 3 cut(s) 230, 634, 889
Bse3DI GCAATG 2 cut(s) 126, 635
Bse8I GATNNNNATC 1 cut(s) 334
BseBI CCWGG 4 cut(s) 31, 444, 557, 609
BseDI CCNNGG 4 cut(s) 30, 442, 443, 608
BseJI GATNNNNATC 1 cut(s) 334
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMI GCAATG 2 cut(s) 126, 635
BseMII CTCAG 4 cut(s) 188, 231, 314, 804
BseNI ACTGG 3 cut(s) 230, 634, 889
BseRI GAGGAG 1 cut(s) 51
BseX3I CGGCCG 1 cut(s) 108
BseXI GCAGC 2 cut(s) 145, 880
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 6 cut(s) 5, 110, 146, 207, 634, 741
BsiEI CGRYCG 1 cut(s) 111
BsiHKAI GWGCWC 2 cut(s) 175, 592
BsiHKCI CYCGRG 1 cut(s) 708
BsiSI CCGG 2 cut(s) 65, 717
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 100
BsmFI GGGAC 1 cut(s) 20
BsnI GGCC 6 cut(s) 5, 110, 146, 207, 634, 741
BsoBI CYCGRG 1 cut(s) 708
Bsp1286I GDGCHC 2 cut(s) 175, 592
Bsp1407I TGTACA 1 cut(s) 360
Bsp143I GATC 4 cut(s) 329, 352, 570, 880
Bsp1720I GCTNAGC 2 cut(s) 174, 300
BspACI CCGC 3 cut(s) 9, 162, 871
BspANI GGCC 6 cut(s) 5, 110, 146, 207, 634, 741
BspCNI CTCAG 4 cut(s) 187, 232, 313, 805
BspLI GGNNCC 4 cut(s) 35, 36, 554, 740
BspMI ACCTGC 2 cut(s) 49, 712
BspPI GGATC 1 cut(s) 337
BsrDI GCAATG 2 cut(s) 126, 635
BsrGI TGTACA 1 cut(s) 360
BsrI ACTGG 3 cut(s) 230, 634, 889
BssECI CCNNGG 4 cut(s) 30, 442, 443, 608
BssMI GATC 4 cut(s) 329, 352, 570, 880
Bst2UI CCWGG 4 cut(s) 31, 444, 557, 609
Bst4CI ACNGT 1 cut(s) 90
Bst6I CTCTTC 1 cut(s) 789
BstAPI GCANNNNNTGC 2 cut(s) 170, 587
BstAUI TGTACA 1 cut(s) 360
BstC8I GCNNGC 3 cut(s) 139, 156, 777
BstDEI CTNAG 5 cut(s) 174, 240, 300, 813, 839
BstH2I RGCGCY 1 cut(s) 307
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 4 cut(s) 332, 355, 573, 883
BstMBI GATC 4 cut(s) 329, 352, 570, 880
BstMCI CGRYCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 4 cut(s) 143, 170, 587, 713
BstNI CCWGG 4 cut(s) 31, 444, 557, 609
BstSCI CCNGG 4 cut(s) 29, 442, 555, 607
BstSFI CTRYAG 2 cut(s) 669, 783
BstV1I GCAGC 2 cut(s) 145, 880
BstX2I RGATCY 1 cut(s) 329
BstXI CCANNNNNNTGG 1 cut(s) 642
BstYI RGATCY 1 cut(s) 329
BstZI CGGCCG 1 cut(s) 108
BsuRI GGCC 6 cut(s) 5, 110, 146, 207, 634, 741
BtsIMutI CAGTG 3 cut(s) 237, 507, 627
BveI ACCTGC 2 cut(s) 49, 712
Cac8I GCNNGC 3 cut(s) 139, 156, 777
CaiI CAGNNNCTG 1 cut(s) 307
CfoI GCGC 1 cut(s) 306
Cfr13I GGNCC 5 cut(s) 34, 206, 632, 739, 758
CseI GACGC 1 cut(s) 260
Csp6I GTAC 2 cut(s) 361, 384
CspCI CAANNNNNGTGG 2 cut(s) 736, 771
CviAII CATG 5 cut(s) 134, 283, 622, 735, 791
CviQI GTAC 2 cut(s) 361, 384
DdeI CTNAG 5 cut(s) 174, 240, 300, 813, 839
DpnI GATC 4 cut(s) 331, 354, 572, 882
DpnII GATC 4 cut(s) 329, 352, 570, 880
DraIII CACNNNGTG 1 cut(s) 170
EaeI YGGCCR 1 cut(s) 108
EagI CGGCCG 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 789
EarI CTCTTC 1 cut(s) 789
EclXI CGGCCG 1 cut(s) 108
Eco32I GATATC 1 cut(s) 439
Eco47I GGWCC 2 cut(s) 34, 758
Eco52I CGGCCG 1 cut(s) 108
Eco57I CTGAAG 3 cut(s) 312, 531, 884
Eco88I CYCGRG 1 cut(s) 708
EcoO109I RGGNCCY 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 913
EcoRII CCWGG 4 cut(s) 29, 442, 555, 607
EcoRV GATATC 1 cut(s) 439
EcoT22I ATGCAT 1 cut(s) 268
FaeI CATG 5 cut(s) 137, 286, 625, 738, 794
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FaqI GGGAC 1 cut(s) 20
FatI CATG 5 cut(s) 133, 282, 621, 734, 790
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 4 cut(s) 159, 162, 869, 872
Fsp4HI GCNGC 4 cut(s) 159, 162, 869, 872
FspBI CTAG 1 cut(s) 563
GlaI GCGC 1 cut(s) 305
GluI GCNGC 4 cut(s) 159, 162, 869, 872
HaeII RGCGCY 1 cut(s) 307
HaeIII GGCC 6 cut(s) 5, 110, 146, 207, 634, 741
HapII CCGG 2 cut(s) 65, 717
HgaI GACGC 1 cut(s) 260
HhaI GCGC 1 cut(s) 306
Hin1II CATG 5 cut(s) 137, 286, 625, 738, 794
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 2 cut(s) 464, 472
HpaII CCGG 2 cut(s) 65, 717
HphI GGTGA 6 cut(s) 115, 272, 307, 362, 693, 890
Hpy166II GTNNAC 4 cut(s) 57, 70, 505, 701
Hpy188I TCNGA 3 cut(s) 292, 340, 906
Hpy188III TCNNGA 1 cut(s) 710
Hpy8I GTNNAC 4 cut(s) 57, 70, 505, 701
HpyAV CCTTC 2 cut(s) 56, 282
HpyCH4III ACNGT 1 cut(s) 90
HpyCH4V TGCA 3 cut(s) 266, 640, 655
HpyF10VI GCNNNNNNNGC 4 cut(s) 143, 170, 587, 713
HpyF3I CTNAG 5 cut(s) 174, 240, 300, 813, 839
Hsp92II CATG 5 cut(s) 137, 286, 625, 738, 794
HspAI GCGC 1 cut(s) 304
KflI GGGWCCC 1 cut(s) 34
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 4 cut(s) 329, 352, 570, 880
LmnI GCTCC 2 cut(s) 578, 719
Lsp1109I GCAGC 2 cut(s) 145, 880
LweI GCATC 3 cut(s) 253, 275, 703
MaeI CTAG 1 cut(s) 563
MaeIII GTNAC 1 cut(s) 103
MalI GATC 4 cut(s) 331, 354, 572, 882
MboI GATC 4 cut(s) 329, 352, 570, 880
MboII GAAGA 5 cut(s) 35, 427, 460, 806, 855
MfeI CAATTG 1 cut(s) 95
MflI RGATCY 1 cut(s) 329
MhlI GDGCHC 2 cut(s) 175, 592
MluCI AATT 3 cut(s) 95, 647, 913
MlyI GAGTC 1 cut(s) 466
MmeI TCCRAC 1 cut(s) 736
Mph1103I ATGCAT 1 cut(s) 268
MseI TTAA 2 cut(s) 78, 618
MspA1I CMGCKG 3 cut(s) 178, 358, 871
MspI CCGG 2 cut(s) 65, 717
MspR9I CCNGG 4 cut(s) 31, 444, 557, 609
MunI CAATTG 1 cut(s) 95
MvaI CCWGG 4 cut(s) 31, 444, 557, 609
MwoI GCNNNNNNNGC 4 cut(s) 143, 170, 587, 713
NdeII GATC 4 cut(s) 329, 352, 570, 880
NlaIII CATG 5 cut(s) 137, 286, 625, 738, 794
NlaIV GGNNCC 4 cut(s) 35, 36, 554, 740
NmuCI GTSAC 1 cut(s) 103
NsiI ATGCAT 1 cut(s) 268
PaeR7I CTCGAG 1 cut(s) 708
PaqCI CACCTGC 2 cut(s) 49, 712
PasI CCCWGGG 1 cut(s) 443
PfeI GAWTC 1 cut(s) 464
PfoI TCCNGGA 1 cut(s) 555
PkrI GCNGC 4 cut(s) 160, 163, 870, 873
PleI GAGTC 1 cut(s) 466
PpsI GAGTC 1 cut(s) 466
PpuMI RGGWCCY 1 cut(s) 34
Psp5II RGGWCCY 1 cut(s) 34
Psp6I CCWGG 4 cut(s) 29, 442, 555, 607
PspGI CCWGG 4 cut(s) 29, 442, 555, 607
PspN4I GGNNCC 4 cut(s) 35, 36, 554, 740
PspPI GGNCC 5 cut(s) 34, 206, 632, 739, 758
PspPPI RGGWCCY 1 cut(s) 34
PstNI CAGNNNCTG 1 cut(s) 307
PsuI RGATCY 1 cut(s) 329
PvuII CAGCTG 2 cut(s) 178, 358
RsaI GTAC 2 cut(s) 362, 385
RsaNI GTAC 2 cut(s) 361, 384
SaqAI TTAA 2 cut(s) 78, 618
SatI GCNGC 4 cut(s) 159, 162, 869, 872
Sau3AI GATC 4 cut(s) 329, 352, 570, 880
Sau96I GGNCC 5 cut(s) 34, 206, 632, 739, 758
SchI GAGTC 1 cut(s) 466
ScrFI CCNGG 4 cut(s) 31, 444, 557, 609
SduI GDGCHC 2 cut(s) 175, 592
SfaNI GCATC 3 cut(s) 253, 275, 703
SfcI CTRYAG 2 cut(s) 669, 783
Sfr274I CTCGAG 1 cut(s) 708
SinI GGWCC 2 cut(s) 34, 758
SlaI CTCGAG 1 cut(s) 708
SmlI CTYRAG 2 cut(s) 708, 833
SmoI CTYRAG 2 cut(s) 708, 833
Sse9I AATT 3 cut(s) 95, 647, 913
SsiI CCGC 3 cut(s) 9, 162, 871
SspMI CTAG 1 cut(s) 563
StyD4I CCNGG 4 cut(s) 29, 442, 555, 607
TaaI ACNGT 1 cut(s) 90
TaqI TCGA 3 cut(s) 150, 275, 709
TasI AATT 3 cut(s) 95, 647, 913
TatI WGTACW 1 cut(s) 360
TauI GCSGC 2 cut(s) 164, 874
TfiI GAWTC 1 cut(s) 464
Tru1I TTAA 2 cut(s) 78, 618
Tru9I TTAA 2 cut(s) 78, 618
TscAI CASTG 3 cut(s) 237, 514, 634
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 2 cut(s) 158, 868
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 1 cut(s) 807
TspRI CASTG 3 cut(s) 237, 514, 634
VpaK11BI GGWCC 2 cut(s) 34, 758
XapI RAATTY 1 cut(s) 913
XhoI CTCGAG 1 cut(s) 708
XspI CTAG 1 cut(s) 563
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.