MD15G1052400.v1.1

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010419
Physical Location & Seq
Reverse (-)
3600783 .. 3603856
3074 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1052400.v1.1.491

Sequence Viewer

Length: 948 bp
ATGGCGGCCGCTTACAAGCCCGAAGAAGCCAGGGTCCCGCCTGCTCTTCCCCTGCCCGCTCCTCCCGTCACCAAGGCAAGCTGTCTCTTTAATCTCTGTTTCAAGATTGCGCTGTGCCAATTGTCGGTGACGCCGGATAAGGAGAGGAACATTGCCCACTCTCGGAAAGCCATCGAGGACGCCGCCGCAAAGGGTGCCCAACTTGTTCTCCTCCCTGAAATTTGGAATGGTCCGTATTCAAATGACAGTTTTCCTGTTTATGCTGAGGACATTGATGCGGGTGGCGATGCATCTCCTTCAACAGCCATGCTGTCTGAAGTTGCTCAACGTCTTAAGATCACCATTGTTGGTGGATCTATAGTGGAGCGTTCTGGGGATCGGTTGTACAATACTTCCTGTGTCTTTGGCACCAATGGAAAGCTGTTAGCTAAGCACAGGAAGATGCAGCTATTTGATATCAACGTCCCGGGGAAGATTACCTTCACTGAATCAAAGACTCTCACAGCAGGGCAGACTCCTACCGTTGTGGATACAGAAGTTGGGCGTATTGGCATAGGTATCTGTTATGACATTAATTTTGAGGAAATAGCAATGATTTATGGAGCAAGAGGTGCTCACTTGATATGCTATCCTGGGGCTTTTAACATGACTACTGGACCGTTGCACTGGGAACTGTTGCAGAGGGCAAGGGCTACAGATAACCAGCTATATGTAGCAACTTGTTCCCCTGCTCGAGATGCTGGAGCCGGTTATGTGGCCTGGGGCCACTCCACCCTTGTTGGACCATTTGGAGAAGTACTGGCGACGACAGAACATGAAGAGGCAATAATCATAGCAGAGATTGATTATTCAATTATTGAGCTTAGAAGGACAAACCTCCCCTTACTGAAGCAAAGACGAGGCGATCTTTACCGGTTGGTAGATGTTGAGAGGTTAAATTCTCAATGA

Protein Analysis

316

Amino Acids

34.3

Weight (kDa)

5.92

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 35 - 285 7.3e-63 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 194, 407
AccBSI CCGCTC 1 cut(s) 59
AciI CCGC 7 cut(s) 5, 9, 38, 57, 183, 186, 278
AclWI GGATC 2 cut(s) 361, 384
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 2 cut(s) 219, 937
AcuI CTGAAG 2 cut(s) 336, 908
AcyI GRCGYC 2 cut(s) 131, 180
AfaI GTAC 2 cut(s) 386, 798
AfiI CCNNNNNNNGG 2 cut(s) 124, 162
AflII CTTAAG 1 cut(s) 332
AgeI ACCGGT 1 cut(s) 912
AgsI TTSAA 4 cut(s) 103, 240, 300, 852
AjnI CCWGG 3 cut(s) 29, 631, 758
AluBI AGCT 6 cut(s) 81, 421, 428, 448, 706, 862
AluI AGCT 6 cut(s) 81, 421, 428, 448, 706, 862
Alw21I GWGCWC 1 cut(s) 616
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 2 cut(s) 361, 384
Ama87I CYCGRG 2 cut(s) 466, 732
AoxI GGCC 3 cut(s) 6, 756, 763
ApeKI GCWGC 1 cut(s) 445
ApoI RAATTY 2 cut(s) 219, 937
ArsI GACNNNNNNTTYG 2 cut(s) 560, 592
AseI ATTAAT 1 cut(s) 573
AsiGI ACCGGT 1 cut(s) 912
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 5 cut(s) 34, 230, 656, 763, 782
AsuC2I CCSGG 2 cut(s) 467, 468
AsuHPI GGTGA 3 cut(s) 61, 139, 331
AvaI CYCGRG 2 cut(s) 466, 732
AvaII GGWCC 4 cut(s) 34, 230, 656, 782
BaeGI GKGCMC 1 cut(s) 199
BaeI ACNNNNGTAYC 2 cut(s) 522, 555
BanI GGYRCC 2 cut(s) 194, 407
Bbv12I GWGCWC 1 cut(s) 616
BbvCI CCTCAGC 1 cut(s) 264
BbvI GCAGC 1 cut(s) 457
BccI CCATC 1 cut(s) 179
BciT130I CCWGG 3 cut(s) 31, 633, 760
BciVI GTATCC 1 cut(s) 523
BcnI CCSGG 2 cut(s) 467, 468
BcoDI GTCTC 1 cut(s) 89
BfmI CTRYAG 2 cut(s) 357, 693
BfrI CTTAAG 1 cut(s) 332
BfuI GTATCC 1 cut(s) 523
BisI GCNGC 5 cut(s) 6, 9, 183, 186, 446
BlpI GCTNAGC 1 cut(s) 429
BlsI GCNGC 5 cut(s) 7, 10, 184, 187, 447
BmcAI AGTACT 1 cut(s) 798
Bme1390I CCNGG 5 cut(s) 31, 467, 468, 633, 760
Bme18I GGWCC 4 cut(s) 34, 230, 656, 782
BmeT110I CYCGRG 2 cut(s) 466, 732
BmgT120I GGNCC 5 cut(s) 34, 230, 656, 763, 782
BmiI GGNNCC 6 cut(s) 35, 36, 196, 409, 745, 764
BmrFI CCNGG 5 cut(s) 31, 467, 468, 633, 760
BmrI ACTGGG 1 cut(s) 676
BmsI GCATC 5 cut(s) 265, 277, 299, 432, 727
BmuI ACTGGG 1 cut(s) 676
BpmI CTGGAG 1 cut(s) 762
Bpu10I CCTNAGC 1 cut(s) 264
Bpu1102I GCTNAGC 1 cut(s) 429
BpuMI CCSGG 2 cut(s) 467, 468
BsaHI GRCGYC 2 cut(s) 131, 180
BsaJI CCNNGG 6 cut(s) 30, 72, 466, 467, 632, 759
BsaWI WCCGGW 1 cut(s) 912
BsaXI ACNNNNNCTCC 2 cut(s) 192, 222
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 162
Bse118I RCCGGY 2 cut(s) 746, 912
Bse1I ACTGG 3 cut(s) 658, 671, 804
Bse3DI GCAATG 2 cut(s) 150, 597
BseBI CCWGG 3 cut(s) 31, 633, 760
BseDI CCNNGG 6 cut(s) 30, 72, 466, 467, 632, 759
BseLI CCNNNNNNNGG 2 cut(s) 124, 162
BseMI GCAATG 2 cut(s) 150, 597
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 3 cut(s) 658, 671, 804
BseRI GAGGAG 2 cut(s) 51, 200
BseSI GKGCMC 1 cut(s) 199
BseX3I CGGCCG 1 cut(s) 6
BseXI GCAGC 1 cut(s) 457
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 3 cut(s) 8, 758, 765
BshNI GGYRCC 2 cut(s) 194, 407
BshTI ACCGGT 1 cut(s) 912
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 616
BsiHKCI CYCGRG 2 cut(s) 466, 732
BsiSI CCGG 4 cut(s) 134, 467, 747, 913
BslFI GGGAC 2 cut(s) 20, 449
BslI CCNNNNNNNGG 2 cut(s) 124, 162
BsmAI GTCTC 1 cut(s) 89
BsmFI GGGAC 2 cut(s) 20, 449
BsnI GGCC 3 cut(s) 8, 758, 765
BsoBI CYCGRG 2 cut(s) 466, 732
Bsp1286I GDGCHC 2 cut(s) 199, 616
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 4 cut(s) 336, 353, 376, 904
Bsp1720I GCTNAGC 1 cut(s) 429
BspACI CCGC 7 cut(s) 5, 9, 38, 57, 183, 186, 278
BspANI GGCC 3 cut(s) 8, 758, 765
BspCNI CTCAG 1 cut(s) 256
BspLI GGNNCC 6 cut(s) 35, 36, 196, 409, 745, 764
BspPI GGATC 2 cut(s) 361, 384
BspQI GCTCTTC 1 cut(s) 51
BspT107I GGYRCC 2 cut(s) 194, 407
BspTI CTTAAG 1 cut(s) 332
BsrBI CCGCTC 1 cut(s) 59
BsrDI GCAATG 2 cut(s) 150, 597
BsrFI RCCGGY 2 cut(s) 746, 912
BsrGI TGTACA 1 cut(s) 384
BsrI ACTGG 3 cut(s) 658, 671, 804
BssAI RCCGGY 2 cut(s) 746, 912
BssECI CCNNGG 6 cut(s) 30, 72, 466, 467, 632, 759
BssMI GATC 4 cut(s) 336, 353, 376, 904
BssNI GRCGYC 2 cut(s) 131, 180
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 3 cut(s) 31, 633, 760
Bst4CI ACNGT 4 cut(s) 248, 523, 660, 675
Bst6I CTCTTC 2 cut(s) 51, 813
BstACI GRCGYC 2 cut(s) 131, 180
BstAFI CTTAAG 1 cut(s) 332
BstAPI GCANNNNNTGC 2 cut(s) 194, 611
BstAUI TGTACA 1 cut(s) 384
BstC8I GCNNGC 3 cut(s) 42, 57, 79
BstDEI CTNAG 3 cut(s) 264, 429, 863
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 4 cut(s) 339, 356, 379, 907
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 4 cut(s) 336, 353, 376, 904
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 3 cut(s) 194, 611, 737
BstNI CCWGG 3 cut(s) 31, 633, 760
BstSCI CCNGG 5 cut(s) 29, 465, 466, 631, 758
BstSFI CTRYAG 2 cut(s) 357, 693
BstSLI GKGCMC 1 cut(s) 199
BstV1I GCAGC 1 cut(s) 457
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
BstZI CGGCCG 1 cut(s) 6
BsuI GTATCC 1 cut(s) 523
BsuRI GGCC 3 cut(s) 8, 758, 765
BtgZI GCGATG 1 cut(s) 300
BtsIMutI CAGTG 2 cut(s) 483, 664
Cac8I GCNNGC 3 cut(s) 42, 57, 79
CciNI GCGGCCGC 1 cut(s) 6
CfoI GCGC 1 cut(s) 112
Cfr10I RCCGGY 2 cut(s) 746, 912
Cfr13I GGNCC 5 cut(s) 34, 230, 656, 763, 782
Cfr9I CCCGGG 1 cut(s) 466
CseI GACGC 2 cut(s) 139, 188
Csp6I GTAC 2 cut(s) 385, 797
CspAI ACCGGT 1 cut(s) 912
CspCI CAANNNNNGTGG 2 cut(s) 760, 795
CviAII CATG 3 cut(s) 307, 646, 815
CviQI GTAC 2 cut(s) 385, 797
DdeI CTNAG 3 cut(s) 264, 429, 863
DpnI GATC 4 cut(s) 338, 355, 378, 906
DpnII GATC 4 cut(s) 336, 353, 376, 904
EaeI YGGCCR 1 cut(s) 6
EagI CGGCCG 1 cut(s) 6
Eam1104I CTCTTC 2 cut(s) 51, 813
EarI CTCTTC 2 cut(s) 51, 813
EclXI CGGCCG 1 cut(s) 6
Eco130I CCWWGG 1 cut(s) 72
Eco32I GATATC 1 cut(s) 457
Eco47I GGWCC 4 cut(s) 34, 230, 656, 782
Eco52I CGGCCG 1 cut(s) 6
Eco57I CTGAAG 2 cut(s) 336, 908
Eco88I CYCGRG 2 cut(s) 466, 732
EcoO109I RGGNCCY 1 cut(s) 34
EcoRII CCWGG 3 cut(s) 29, 631, 758
EcoRV GATATC 1 cut(s) 457
EcoT14I CCWWGG 1 cut(s) 72
EcoT22I ATGCAT 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 3 cut(s) 310, 649, 818
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FaqI GGGAC 2 cut(s) 20, 449
FatI CATG 3 cut(s) 306, 645, 814
FauI CCCGC 3 cut(s) 45, 64, 271
Fnu4HI GCNGC 5 cut(s) 6, 9, 183, 186, 446
Fsp4HI GCNGC 5 cut(s) 6, 9, 183, 186, 446
GlaI GCGC 1 cut(s) 111
GluI GCNGC 5 cut(s) 6, 9, 183, 186, 446
GsuI CTGGAG 1 cut(s) 762
HaeIII GGCC 3 cut(s) 8, 758, 765
HapII CCGG 4 cut(s) 134, 467, 747, 913
HgaI GACGC 2 cut(s) 139, 188
HhaI GCGC 1 cut(s) 112
Hin1I GRCGYC 2 cut(s) 131, 180
Hin1II CATG 3 cut(s) 310, 649, 818
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HinfI GANTC 3 cut(s) 488, 496, 514
HpaII CCGG 4 cut(s) 134, 467, 747, 913
HphI GGTGA 3 cut(s) 61, 139, 331
Hpy188I TCNGA 2 cut(s) 165, 316
Hpy188III TCNNGA 2 cut(s) 103, 734
Hpy99I CGWCG 1 cut(s) 808
HpyAV CCTTC 3 cut(s) 306, 490, 861
HpyCH4III ACNGT 4 cut(s) 248, 523, 660, 675
HpyCH4IV ACGT 2 cut(s) 328, 462
HpyCH4V TGCA 4 cut(s) 290, 445, 664, 679
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 611, 737
HpyF3I CTNAG 3 cut(s) 264, 429, 863
HpySE526I ACGT 2 cut(s) 328, 462
Hsp92I GRCGYC 2 cut(s) 131, 180
Hsp92II CATG 3 cut(s) 310, 649, 818
HspAI GCGC 1 cut(s) 110
KflI GGGWCCC 1 cut(s) 34
Kzo9I GATC 4 cut(s) 336, 353, 376, 904
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 4 cut(s) 64, 364, 602, 743
Lsp1109I GCAGC 1 cut(s) 457
LweI GCATC 5 cut(s) 265, 277, 299, 432, 727
MaeII ACGT 2 cut(s) 328, 462
MaeIII GTNAC 2 cut(s) 67, 127
MalI GATC 4 cut(s) 338, 355, 378, 906
MbiI CCGCTC 1 cut(s) 59
MboI GATC 4 cut(s) 336, 353, 376, 904
MboII GAAGA 5 cut(s) 35, 38, 451, 484, 830
MfeI CAATTG 1 cut(s) 119
MflI RGATCY 1 cut(s) 353
MhlI GDGCHC 2 cut(s) 199, 616
MluCI AATT 5 cut(s) 119, 219, 574, 852, 937
MlyI GAGTC 2 cut(s) 490, 508
MmeI TCCRAC 1 cut(s) 760
Mph1103I ATGCAT 1 cut(s) 292
MseI TTAA 5 cut(s) 90, 333, 573, 642, 935
MspCI CTTAAG 1 cut(s) 332
MspI CCGG 4 cut(s) 134, 467, 747, 913
MspR9I CCNGG 5 cut(s) 31, 467, 468, 633, 760
MunI CAATTG 1 cut(s) 119
MvaI CCWGG 3 cut(s) 31, 633, 760
MwoI GCNNNNNNNGC 3 cut(s) 194, 611, 737
NciI CCSGG 2 cut(s) 467, 468
NdeII GATC 4 cut(s) 336, 353, 376, 904
NlaIII CATG 3 cut(s) 310, 649, 818
NlaIV GGNNCC 6 cut(s) 35, 36, 196, 409, 745, 764
NmuCI GTSAC 2 cut(s) 67, 127
NotI GCGGCCGC 1 cut(s) 6
NsiI ATGCAT 1 cut(s) 292
PaeR7I CTCGAG 1 cut(s) 732
PciSI GCTCTTC 1 cut(s) 51
PfeI GAWTC 1 cut(s) 488
PinAI ACCGGT 1 cut(s) 912
PkrI GCNGC 5 cut(s) 7, 10, 184, 187, 447
PleI GAGTC 2 cut(s) 490, 508
PpsI GAGTC 2 cut(s) 490, 508
PpuMI RGGWCCY 1 cut(s) 34
PshBI ATTAAT 1 cut(s) 573
Psp5II RGGWCCY 1 cut(s) 34
Psp6I CCWGG 3 cut(s) 29, 631, 758
PspGI CCWGG 3 cut(s) 29, 631, 758
PspN4I GGNNCC 6 cut(s) 35, 36, 196, 409, 745, 764
PspPI GGNCC 5 cut(s) 34, 230, 656, 763, 782
PspPPI RGGWCCY 1 cut(s) 34
PsuI RGATCY 1 cut(s) 353
RsaI GTAC 2 cut(s) 386, 798
RsaNI GTAC 2 cut(s) 385, 797
SapI GCTCTTC 1 cut(s) 51
SaqAI TTAA 5 cut(s) 90, 333, 573, 642, 935
SatI GCNGC 5 cut(s) 6, 9, 183, 186, 446
Sau3AI GATC 4 cut(s) 336, 353, 376, 904
Sau96I GGNCC 5 cut(s) 34, 230, 656, 763, 782
ScaI AGTACT 1 cut(s) 798
SchI GAGTC 2 cut(s) 490, 508
ScrFI CCNGG 5 cut(s) 31, 467, 468, 633, 760
SduI GDGCHC 2 cut(s) 199, 616
SfaNI GCATC 5 cut(s) 265, 277, 299, 432, 727
SfcI CTRYAG 2 cut(s) 357, 693
Sfr274I CTCGAG 1 cut(s) 732
SinI GGWCC 4 cut(s) 34, 230, 656, 782
SlaI CTCGAG 1 cut(s) 732
SmaI CCCGGG 1 cut(s) 468
SmlI CTYRAG 2 cut(s) 332, 732
SmoI CTYRAG 2 cut(s) 332, 732
Sse9I AATT 5 cut(s) 119, 219, 574, 852, 937
SsiI CCGC 7 cut(s) 5, 9, 38, 57, 183, 186, 278
StyD4I CCNGG 5 cut(s) 29, 465, 466, 631, 758
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 4 cut(s) 248, 523, 660, 675
TaiI ACGT 2 cut(s) 331, 465
TaqI TCGA 2 cut(s) 174, 733
TasI AATT 5 cut(s) 119, 219, 574, 852, 937
TatI WGTACW 2 cut(s) 384, 796
TauI GCSGC 4 cut(s) 8, 11, 185, 188
TfiI GAWTC 1 cut(s) 488
Tru1I TTAA 5 cut(s) 90, 333, 573, 642, 935
Tru9I TTAA 5 cut(s) 90, 333, 573, 642, 935
TscAI CASTG 2 cut(s) 490, 671
TseFI GTSAC 2 cut(s) 67, 127
TseI GCWGC 1 cut(s) 445
Tsp45I GTSAC 2 cut(s) 67, 127
TspDTI ATGAA 1 cut(s) 831
TspGWI ACGGA 1 cut(s) 222
TspMI CCCGGG 1 cut(s) 466
TspRI CASTG 2 cut(s) 490, 671
Vha464I CTTAAG 1 cut(s) 332
VpaK11BI GGWCC 4 cut(s) 34, 230, 656, 782
VspI ATTAAT 1 cut(s) 573
XapI RAATTY 2 cut(s) 219, 937
XhoI CTCGAG 1 cut(s) 732
XmaI CCCGGG 1 cut(s) 466
ZrmI AGTACT 1 cut(s) 798
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.