MD15G1052500.v1.1

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010419
Physical Location & Seq
Reverse (-)
3604050 .. 3607043
2994 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1052500.v1.1.491

Sequence Viewer

Length: 948 bp
ATGGCGTCGGCTTACAAGCCCGAAGAAGCCAGGGTCCCGCCTGCTCTTCCCCTGCCCACTCCTCCTGTCACCAAGGCAAGCTGTCTCTTTAATCTCTCTTTTAAGATTGCGCTGTGCCAATTGTCGGTGACGCCGGATAAGGAGAGGAACATTTCCCACGCTCGGAAAGCCATCGAAGACGCCGCCGCAAAGGGTGCCCAGCTTGTCGTCCTCCCTGAAATTTGGAATGGTCCGTATTCAAATGACAGCTTTCCGGTTTATGCTGAGGACATTGATGCGGGTGGCGATGCATCTCCTTCAACAGCCATGCTGTCTGAAGTTGCTCAACGTCTGAAGATCACCATTGTTGGTGGATCTATAGCGGAGCGTTCTGGGGATCGGTTGTACAATACTTCCTGTGTCTTTGACACCGATGGAAAGCTGTTAGCTAAACACAGGAAGATACACCTATTTGATATCGACATCCCGGGGAAGATTACCTTCATTGAATCAAAGACTCTCACAGCAGGGCAGACTCCTACCATTGTGGATACAGAAGTTGGGCGTATTGGCATAGGTATCTGTTATGACATTAGGTTTCAGGAACTAGCAATGATTTATGCAGCAAGAGGTGCTCACTTGATATGCTATCCTGGGGCTTTTAACATGACTACTGGACCGTTGCACTGGGAATTATTGCAGAGGGCAAGGGCTACAGATAACCAGCTATATGTAGCAACTTGTTCCCCTGCTCGAGATGCTGGAGCCGGTTATGTGGCATGGGGCCACTCAACCCTTGTTGGACCATTTGGAGAAGTAATGGCGACGACAGAACATGAAGAGTCAATAATCATAGCAGAGATTGATTATTCAATTATTGAGCTTAGAAGGACAAACCTCCCCTTACTGAAGCAAAGACGAGGCGATCTTTACCGGTTGGTAGATGTTGAGAGGTTAAATTCTCGATGA

Protein Analysis

316

Amino Acids

34.5

Weight (kDa)

6.01

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 35 - 285 1.1e-66 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 194
AciI CCGC 5 cut(s) 38, 183, 186, 278, 362
AclWI GGATC 2 cut(s) 361, 384
AcsI RAATTY 2 cut(s) 219, 937
AcuI CTGAAG 3 cut(s) 336, 353, 908
AcyI GRCGYC 3 cut(s) 5, 131, 180
AfaI GTAC 1 cut(s) 386
AfiI CCNNNNNNNGG 2 cut(s) 124, 162
AgeI ACCGGT 1 cut(s) 912
AgsI TTSAA 4 cut(s) 240, 300, 488, 852
AjnI CCWGG 2 cut(s) 29, 631
AluBI AGCT 7 cut(s) 81, 202, 249, 421, 428, 706, 862
AluI AGCT 7 cut(s) 81, 202, 249, 421, 428, 706, 862
Alw21I GWGCWC 1 cut(s) 616
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 2 cut(s) 361, 384
Ama87I CYCGRG 2 cut(s) 466, 732
AoxI GGCC 1 cut(s) 763
ApeKI GCWGC 1 cut(s) 602
ApoI RAATTY 2 cut(s) 219, 937
AsiGI ACCGGT 1 cut(s) 912
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 5 cut(s) 34, 230, 656, 763, 782
AsuC2I CCSGG 2 cut(s) 467, 468
AsuHPI GGTGA 3 cut(s) 61, 139, 331
AvaI CYCGRG 2 cut(s) 466, 732
AvaII GGWCC 4 cut(s) 34, 230, 656, 782
BaeGI GKGCMC 1 cut(s) 199
BaeI ACNNNNGTAYC 2 cut(s) 522, 555
BanI GGYRCC 1 cut(s) 194
BbsI GAAGAC 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 616
BbvCI CCTCAGC 1 cut(s) 264
BbvI GCAGC 1 cut(s) 614
BccI CCATC 2 cut(s) 179, 407
BciT130I CCWGG 2 cut(s) 31, 633
BciVI GTATCC 1 cut(s) 523
BcnI CCSGG 2 cut(s) 467, 468
BcoDI GTCTC 1 cut(s) 89
BfaI CTAG 1 cut(s) 587
BfmI CTRYAG 2 cut(s) 357, 693
BfuI GTATCC 1 cut(s) 523
BisI GCNGC 3 cut(s) 183, 186, 603
BlsI GCNGC 3 cut(s) 184, 187, 604
Bme1390I CCNGG 4 cut(s) 31, 467, 468, 633
Bme18I GGWCC 4 cut(s) 34, 230, 656, 782
BmeT110I CYCGRG 2 cut(s) 466, 732
BmgT120I GGNCC 5 cut(s) 34, 230, 656, 763, 782
BmiI GGNNCC 5 cut(s) 35, 36, 196, 745, 764
BmrFI CCNGG 4 cut(s) 31, 467, 468, 633
BmrI ACTGGG 1 cut(s) 676
BmsI GCATC 4 cut(s) 265, 277, 299, 727
BmuI ACTGGG 1 cut(s) 676
BpiI GAAGAC 1 cut(s) 183
BpmI CTGGAG 1 cut(s) 762
Bpu10I CCTNAGC 1 cut(s) 264
BpuMI CCSGG 2 cut(s) 467, 468
BsaHI GRCGYC 3 cut(s) 5, 131, 180
BsaJI CCNNGG 5 cut(s) 30, 72, 466, 467, 632
BsaWI WCCGGW 2 cut(s) 253, 912
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 162
Bse118I RCCGGY 2 cut(s) 746, 912
Bse1I ACTGG 2 cut(s) 658, 671
Bse3DI GCAATG 1 cut(s) 597
BseBI CCWGG 2 cut(s) 31, 633
BseDI CCNNGG 5 cut(s) 30, 72, 466, 467, 632
BseGI GGATG 1 cut(s) 462
BseLI CCNNNNNNNGG 2 cut(s) 124, 162
BseMI GCAATG 1 cut(s) 597
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 2 cut(s) 658, 671
BseRI GAGGAG 1 cut(s) 51
BseSI GKGCMC 1 cut(s) 199
BseXI GCAGC 1 cut(s) 614
BseYI CCCAGC 1 cut(s) 198
BshFI GGCC 1 cut(s) 765
BshNI GGYRCC 1 cut(s) 194
BshTI ACCGGT 1 cut(s) 912
BsiHKAI GWGCWC 1 cut(s) 616
BsiHKCI CYCGRG 2 cut(s) 466, 732
BsiSI CCGG 5 cut(s) 134, 254, 467, 747, 913
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 2 cut(s) 124, 162
BsmAI GTCTC 1 cut(s) 89
BsmFI GGGAC 1 cut(s) 20
BsnI GGCC 1 cut(s) 765
BsoBI CYCGRG 2 cut(s) 466, 732
Bsp1286I GDGCHC 2 cut(s) 199, 616
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 4 cut(s) 336, 353, 376, 904
BspACI CCGC 5 cut(s) 38, 183, 186, 278, 362
BspANI GGCC 1 cut(s) 765
BspCNI CTCAG 1 cut(s) 256
BspLI GGNNCC 5 cut(s) 35, 36, 196, 745, 764
BspPI GGATC 2 cut(s) 361, 384
BspQI GCTCTTC 1 cut(s) 51
BspT107I GGYRCC 1 cut(s) 194
BsrDI GCAATG 1 cut(s) 597
BsrFI RCCGGY 2 cut(s) 746, 912
BsrGI TGTACA 1 cut(s) 384
BsrI ACTGG 2 cut(s) 658, 671
BssAI RCCGGY 2 cut(s) 746, 912
BssECI CCNNGG 5 cut(s) 30, 72, 466, 467, 632
BssMI GATC 4 cut(s) 336, 353, 376, 904
BssNI GRCGYC 3 cut(s) 5, 131, 180
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 2 cut(s) 31, 633
Bst4CI ACNGT 1 cut(s) 660
Bst6I CTCTTC 2 cut(s) 51, 813
BstACI GRCGYC 3 cut(s) 5, 131, 180
BstAPI GCANNNNNTGC 2 cut(s) 194, 611
BstAUI TGTACA 1 cut(s) 384
BstC8I GCNNGC 2 cut(s) 42, 79
BstDEI CTNAG 2 cut(s) 264, 863
BstF5I GGATG 1 cut(s) 462
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 4 cut(s) 339, 356, 379, 907
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 4 cut(s) 336, 353, 376, 904
BstMWI GCNNNNNNNGC 4 cut(s) 167, 194, 611, 737
BstNI CCWGG 2 cut(s) 31, 633
BstSCI CCNGG 4 cut(s) 29, 465, 466, 631
BstSFI CTRYAG 2 cut(s) 357, 693
BstSLI GKGCMC 1 cut(s) 199
BstV1I GCAGC 1 cut(s) 614
BstV2I GAAGAC 1 cut(s) 183
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
BsuI GTATCC 1 cut(s) 523
BsuRI GGCC 1 cut(s) 765
BtgZI GCGATG 1 cut(s) 300
BtsCI GGATG 1 cut(s) 462
BtsIMutI CAGTG 1 cut(s) 664
Cac8I GCNNGC 2 cut(s) 42, 79
CfoI GCGC 1 cut(s) 112
Cfr10I RCCGGY 2 cut(s) 746, 912
Cfr13I GGNCC 5 cut(s) 34, 230, 656, 763, 782
Cfr9I CCCGGG 1 cut(s) 466
CseI GACGC 2 cut(s) 139, 188
Csp6I GTAC 1 cut(s) 385
CspAI ACCGGT 1 cut(s) 912
CviAII CATG 4 cut(s) 307, 646, 759, 815
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 2 cut(s) 264, 863
DpnI GATC 4 cut(s) 338, 355, 378, 906
DpnII GATC 4 cut(s) 336, 353, 376, 904
Eam1104I CTCTTC 2 cut(s) 51, 813
EarI CTCTTC 2 cut(s) 51, 813
Eco130I CCWWGG 1 cut(s) 72
Eco32I GATATC 1 cut(s) 457
Eco47I GGWCC 4 cut(s) 34, 230, 656, 782
Eco57I CTGAAG 3 cut(s) 336, 353, 908
Eco88I CYCGRG 2 cut(s) 466, 732
EcoO109I RGGNCCY 1 cut(s) 34
EcoRII CCWGG 2 cut(s) 29, 631
EcoRV GATATC 1 cut(s) 457
EcoT14I CCWWGG 1 cut(s) 72
EcoT22I ATGCAT 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 4 cut(s) 310, 649, 762, 818
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FaqI GGGAC 1 cut(s) 20
FatI CATG 4 cut(s) 306, 645, 758, 814
FauI CCCGC 2 cut(s) 45, 271
Fnu4HI GCNGC 3 cut(s) 183, 186, 603
FokI GGATG 1 cut(s) 449
Fsp4HI GCNGC 3 cut(s) 183, 186, 603
FspBI CTAG 1 cut(s) 587
GlaI GCGC 1 cut(s) 111
GluI GCNGC 3 cut(s) 183, 186, 603
GsaI CCCAGC 1 cut(s) 202
GsuI CTGGAG 1 cut(s) 762
HaeIII GGCC 1 cut(s) 765
HapII CCGG 5 cut(s) 134, 254, 467, 747, 913
HgaI GACGC 2 cut(s) 139, 188
HhaI GCGC 1 cut(s) 112
Hin1I GRCGYC 3 cut(s) 5, 131, 180
Hin1II CATG 4 cut(s) 310, 649, 762, 818
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HinfI GANTC 4 cut(s) 488, 496, 514, 821
HpaII CCGG 5 cut(s) 134, 254, 467, 747, 913
HphI GGTGA 3 cut(s) 61, 139, 331
Hpy188I TCNGA 3 cut(s) 165, 316, 333
Hpy188III TCNNGA 3 cut(s) 581, 734, 942
Hpy99I CGWCG 2 cut(s) 10, 808
HpyAV CCTTC 3 cut(s) 306, 490, 861
HpyCH4III ACNGT 1 cut(s) 660
HpyCH4IV ACGT 1 cut(s) 328
HpyCH4V TGCA 4 cut(s) 290, 602, 664, 679
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 194, 611, 737
HpyF3I CTNAG 2 cut(s) 264, 863
HpySE526I ACGT 1 cut(s) 328
Hsp92I GRCGYC 3 cut(s) 5, 131, 180
Hsp92II CATG 4 cut(s) 310, 649, 762, 818
HspAI GCGC 1 cut(s) 110
KflI GGGWCCC 1 cut(s) 34
Kzo9I GATC 4 cut(s) 336, 353, 376, 904
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 2 cut(s) 364, 743
Lsp1109I GCAGC 1 cut(s) 614
LweI GCATC 4 cut(s) 265, 277, 299, 727
MaeI CTAG 1 cut(s) 587
MaeII ACGT 1 cut(s) 328
MaeIII GTNAC 2 cut(s) 67, 127
MalI GATC 4 cut(s) 338, 355, 378, 906
MboI GATC 4 cut(s) 336, 353, 376, 904
MboII GAAGA 7 cut(s) 35, 38, 188, 346, 451, 484, 830
MfeI CAATTG 1 cut(s) 119
MflI RGATCY 1 cut(s) 353
MhlI GDGCHC 2 cut(s) 199, 616
MluCI AATT 5 cut(s) 119, 219, 671, 852, 937
MlyI GAGTC 3 cut(s) 490, 508, 830
MmeI TCCRAC 1 cut(s) 760
MnlI CCTC 9 cut(s) 72, 138, 221, 259, 602, 675, 887, 893, 924
Mph1103I ATGCAT 1 cut(s) 292
MseI TTAA 4 cut(s) 90, 102, 642, 935
MspI CCGG 5 cut(s) 134, 254, 467, 747, 913
MspR9I CCNGG 4 cut(s) 31, 467, 468, 633
MunI CAATTG 1 cut(s) 119
MvaI CCWGG 2 cut(s) 31, 633
MwoI GCNNNNNNNGC 4 cut(s) 167, 194, 611, 737
NciI CCSGG 2 cut(s) 467, 468
NdeII GATC 4 cut(s) 336, 353, 376, 904
NlaIII CATG 4 cut(s) 310, 649, 762, 818
NlaIV GGNNCC 5 cut(s) 35, 36, 196, 745, 764
NmuCI GTSAC 2 cut(s) 67, 127
NsiI ATGCAT 1 cut(s) 292
PaeR7I CTCGAG 1 cut(s) 732
PciSI GCTCTTC 1 cut(s) 51
PfeI GAWTC 1 cut(s) 488
PinAI ACCGGT 1 cut(s) 912
PkrI GCNGC 3 cut(s) 184, 187, 604
PleI GAGTC 3 cut(s) 490, 508, 829
PpsI GAGTC 3 cut(s) 490, 508, 829
PpuMI RGGWCCY 1 cut(s) 34
Psp5II RGGWCCY 1 cut(s) 34
Psp6I CCWGG 2 cut(s) 29, 631
PspFI CCCAGC 1 cut(s) 198
PspGI CCWGG 2 cut(s) 29, 631
PspN4I GGNNCC 5 cut(s) 35, 36, 196, 745, 764
PspPI GGNCC 5 cut(s) 34, 230, 656, 763, 782
PspPPI RGGWCCY 1 cut(s) 34
PsuI RGATCY 1 cut(s) 353
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
SapI GCTCTTC 1 cut(s) 51
SaqAI TTAA 4 cut(s) 90, 102, 642, 935
SatI GCNGC 3 cut(s) 183, 186, 603
Sau3AI GATC 4 cut(s) 336, 353, 376, 904
Sau96I GGNCC 5 cut(s) 34, 230, 656, 763, 782
SchI GAGTC 3 cut(s) 490, 508, 830
ScrFI CCNGG 4 cut(s) 31, 467, 468, 633
SduI GDGCHC 2 cut(s) 199, 616
SfaNI GCATC 4 cut(s) 265, 277, 299, 727
SfcI CTRYAG 2 cut(s) 357, 693
Sfr274I CTCGAG 1 cut(s) 732
SinI GGWCC 4 cut(s) 34, 230, 656, 782
SlaI CTCGAG 1 cut(s) 732
SmaI CCCGGG 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 732
SmoI CTYRAG 1 cut(s) 732
Sse9I AATT 5 cut(s) 119, 219, 671, 852, 937
SsiI CCGC 5 cut(s) 38, 183, 186, 278, 362
SspMI CTAG 1 cut(s) 587
StyD4I CCNGG 4 cut(s) 29, 465, 466, 631
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 1 cut(s) 660
TaiI ACGT 1 cut(s) 331
TaqI TCGA 4 cut(s) 174, 459, 733, 943
TasI AATT 5 cut(s) 119, 219, 671, 852, 937
TatI WGTACW 1 cut(s) 384
TauI GCSGC 2 cut(s) 185, 188
TfiI GAWTC 1 cut(s) 488
Tru1I TTAA 4 cut(s) 90, 102, 642, 935
Tru9I TTAA 4 cut(s) 90, 102, 642, 935
TscAI CASTG 1 cut(s) 671
TseFI GTSAC 2 cut(s) 67, 127
TseI GCWGC 1 cut(s) 602
Tsp45I GTSAC 2 cut(s) 67, 127
TspDTI ATGAA 2 cut(s) 472, 831
TspGWI ACGGA 1 cut(s) 222
TspMI CCCGGG 1 cut(s) 466
TspRI CASTG 1 cut(s) 671
VpaK11BI GGWCC 4 cut(s) 34, 230, 656, 782
XapI RAATTY 2 cut(s) 219, 937
XhoI CTCGAG 1 cut(s) 732
XmaI CCCGGG 1 cut(s) 466
XspI CTAG 1 cut(s) 587
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.