Rw6G043920

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010419
Physical Location & Seq
Forward (+)
67042840 .. 67047273
4434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G043920.1

Sequence Viewer

Length: 1131 bp
ATGAAAGCAGCACTATCCACACTGATCAGCTCCAAGACTCTCAGCCCTCACCCCTCTTCTTCTTCTTTCTCTCGCTCCTTTTGGTTTCAACCAAAGACCCTCTCCAACTCACCCTCCTTAATTACCCTTATCCCCTCCAAGACCCTCAACACCAACAATTACTCCCGTCTCCGCCTCAATCATCGCAAGTCCCTCACCTCGTCGATAATGGCCTCCGCTTACAAGCCCGAAGAAGCCAGGGTCCCACCTGCCCTTCCCTTGCCCACTCCTCCGGTCAACAAGTTTAAGATTGGACTGTGCCAATTGTCGGTGACGGCCGATAAGGAGAGGAACATTGCTCATGCTCGCAAGGCCATCGAGCAGGCTGCCGCACAGGGTGCTCAGCTGGTTCTCCTACCTGAAATATGGAATGGCCCATATTCAAATGATAGCTTCCCAGTGTTTGCTGAGGATATTGACGCTGGTGGTGATGCTTCTCCTTCGACAGCCATGCTTTCTGAAGTTGCTCAGCGCCTGAAAATCACCATTGTTGGTGGATCTATATCAGAGCGTTCCGGTGATCAGCTGTACAATACTTGCTGTGTCTTTGGTACTGATGGAAAGCTGAAAGCTAAACATAGGAAGATACACCTCTTTGATATTGATATCCCTGGGAAGATTACCTTTATTGAATCAAAGACTCTTACAGCAGGGCAAACTCCTACCATTGTGGACACAGAAGTTGGGCGTATTGGCATAGGCATTTGTTATGACATTAGGTTCCAGGAACTAGCTATGATCTATGGAGCAAGAGGTGCTCACTTGATATGCTATCCTGGTGCTTTTAACATGACCACTGGGCCATTGCATTGGGAATTGTTGCAGAGAGCAAGGGCTACAGATAACCAGCTATATGTGGCAACTTGTTCACCTGCTCGAGATGCCGGAGCTGGTTATGTGGCATGGGGCCACTCCACCCTTGTCGGACCATTTGGAGAAGTGCTGGCAACTACAGAACATGAAGAGGCAATAATCATAGCTGAGGTTGATTATTCACTCCTTGAGCTTAGAAGAACAAATCTCCCTTTACTGAAGCAGCGGCGAGGTGATCTTTACCAGTTGGTAGATGTTCAGAGGTTGAATTCTAAATGA

Protein Analysis

376

Amino Acids

41.12

Weight (kDa)

8.92

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 96 - 348 2.2e-64 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 256, 919
Acc36I ACCTGC 2 cut(s) 256, 919
AciI CCGC 4 cut(s) 172, 216, 369, 1078
AclWI GGATC 1 cut(s) 544
AcoI YGGCCR 1 cut(s) 315
AcsI RAATTY 1 cut(s) 1120
AcuI CTGAAG 2 cut(s) 519, 1091
AdeI CACNNNGTG 1 cut(s) 377
AfaI GTAC 2 cut(s) 569, 592
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 4 cut(s) 89, 423, 671, 1120
AjnI CCWGG 4 cut(s) 236, 649, 762, 814
Alw21I GWGCWC 2 cut(s) 382, 799
Alw26I GTCTC 1 cut(s) 173
AlwI GGATC 1 cut(s) 544
AlwNI CAGNNNCTG 1 cut(s) 514
Ama87I CYCGRG 1 cut(s) 915
AoxI GGCC 6 cut(s) 210, 315, 351, 412, 839, 946
ApeKI GCWGC 3 cut(s) 8, 365, 1075
ApoI RAATTY 1 cut(s) 1120
AspLEI GCGC 1 cut(s) 513
AspS9I GGNCC 5 cut(s) 241, 413, 839, 946, 965
AsuHPI GGTGA 9 cut(s) 41, 102, 187, 322, 479, 514, 569, 900, 1097
AvaI CYCGRG 1 cut(s) 915
AvaII GGWCC 2 cut(s) 241, 965
Bbv12I GWGCWC 2 cut(s) 382, 799
BbvCI CCTCAGC 2 cut(s) 447, 1020
BbvI GCAGC 3 cut(s) 20, 352, 1087
BccI CCATC 2 cut(s) 362, 590
BceAI ACGGC 1 cut(s) 330
BcgI CGANNNNNNTGC 6 cut(s) 337, 347, 371, 381, 472, 506
BciT130I CCWGG 4 cut(s) 238, 651, 764, 816
BclI TGATCA 2 cut(s) 24, 559
BcoDI GTCTC 1 cut(s) 173
BfaI CTAG 1 cut(s) 770
BfmI CTRYAG 2 cut(s) 876, 990
BfoI RGCGCY 1 cut(s) 514
BfuAI ACCTGC 2 cut(s) 256, 919
BisI GCNGC 5 cut(s) 9, 366, 369, 1076, 1079
BlpI GCTNAGC 2 cut(s) 381, 507
BlsI GCNGC 5 cut(s) 10, 367, 370, 1077, 1080
Bme1390I CCNGG 4 cut(s) 238, 651, 764, 816
Bme18I GGWCC 2 cut(s) 241, 965
BmeT110I CYCGRG 1 cut(s) 915
BmgT120I GGNCC 5 cut(s) 241, 413, 839, 946, 965
BmiI GGNNCC 4 cut(s) 242, 243, 761, 947
BmrFI CCNGG 4 cut(s) 238, 651, 764, 816
BmrI ACTGGG 2 cut(s) 431, 846
BmsI GCATC 2 cut(s) 460, 910
BmuI ACTGGG 2 cut(s) 431, 846
Bpu10I CCTNAGC 2 cut(s) 447, 1020
Bpu1102I GCTNAGC 2 cut(s) 381, 507
BpuEI CTTGAG 1 cut(s) 1061
BsaBI GATNNNNATC 1 cut(s) 541
BsaJI CCNNGG 3 cut(s) 237, 649, 650
BsaWI WCCGGW 2 cut(s) 271, 554
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 146, 176
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse1I ACTGG 3 cut(s) 437, 841, 1096
Bse3DI GCAATG 2 cut(s) 333, 842
Bse8I GATNNNNATC 1 cut(s) 541
BseBI CCWGG 4 cut(s) 238, 651, 764, 816
BseDI CCNNGG 3 cut(s) 237, 649, 650
BseJI GATNNNNATC 1 cut(s) 541
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMI GCAATG 2 cut(s) 333, 842
BseMII CTCAG 5 cut(s) 55, 395, 438, 521, 1011
BseNI ACTGG 3 cut(s) 437, 841, 1096
BseRI GAGGAG 1 cut(s) 258
BseX3I CGGCCG 1 cut(s) 315
BseXI GCAGC 3 cut(s) 20, 352, 1087
Bsh1285I CGRYCG 1 cut(s) 318
BshFI GGCC 6 cut(s) 212, 317, 353, 414, 841, 948
BsiEI CGRYCG 1 cut(s) 318
BsiHKAI GWGCWC 2 cut(s) 382, 799
BsiHKCI CYCGRG 1 cut(s) 915
BsiSI CCGG 3 cut(s) 272, 555, 924
BslFI GGGAC 2 cut(s) 175, 227
BslI CCNNNNNNNGG 1 cut(s) 307
BsmAI GTCTC 1 cut(s) 173
BsmBI CGTCTC 1 cut(s) 173
BsmFI GGGAC 2 cut(s) 175, 227
BsnI GGCC 6 cut(s) 212, 317, 353, 414, 841, 948
BsoBI CYCGRG 1 cut(s) 915
Bsp1286I GDGCHC 2 cut(s) 382, 799
Bsp1407I TGTACA 1 cut(s) 567
Bsp143I GATC 5 cut(s) 24, 536, 559, 777, 1087
Bsp1720I GCTNAGC 2 cut(s) 381, 507
BspACI CCGC 4 cut(s) 172, 216, 369, 1078
BspANI GGCC 6 cut(s) 212, 317, 353, 414, 841, 948
BspCNI CTCAG 5 cut(s) 54, 394, 439, 520, 1012
BspLI GGNNCC 4 cut(s) 242, 243, 761, 947
BspMI ACCTGC 2 cut(s) 256, 919
BspPI GGATC 1 cut(s) 544
BsrDI GCAATG 2 cut(s) 333, 842
BsrGI TGTACA 1 cut(s) 567
BsrI ACTGG 3 cut(s) 437, 841, 1096
BssECI CCNNGG 3 cut(s) 237, 649, 650
BssMI GATC 5 cut(s) 24, 536, 559, 777, 1087
Bst2UI CCWGG 4 cut(s) 238, 651, 764, 816
Bst4CI ACNGT 1 cut(s) 297
Bst6I CTCTTC 2 cut(s) 61, 996
BstAPI GCANNNNNTGC 2 cut(s) 377, 794
BstAUI TGTACA 1 cut(s) 567
BstC8I GCNNGC 3 cut(s) 346, 363, 984
BstDEI CTNAG 6 cut(s) 41, 381, 447, 507, 1020, 1046
BstH2I RGCGCY 1 cut(s) 514
BstHHI GCGC 1 cut(s) 513
BstKTI GATC 5 cut(s) 27, 539, 562, 780, 1090
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 5 cut(s) 24, 536, 559, 777, 1087
BstMCI CGRYCG 1 cut(s) 318
BstMWI GCNNNNNNNGC 4 cut(s) 350, 377, 794, 920
BstNI CCWGG 4 cut(s) 238, 651, 764, 816
BstSCI CCNGG 4 cut(s) 236, 649, 762, 814
BstSFI CTRYAG 2 cut(s) 876, 990
BstV1I GCAGC 3 cut(s) 20, 352, 1087
BstX2I RGATCY 1 cut(s) 536
BstXI CCANNNNNNTGG 1 cut(s) 849
BstYI RGATCY 1 cut(s) 536
BstZI CGGCCG 1 cut(s) 315
BsuRI GGCC 6 cut(s) 212, 317, 353, 414, 841, 948
BtgZI GCGATG 1 cut(s) 167
BtsIMutI CAGTG 3 cut(s) 20, 444, 834
BveI ACCTGC 2 cut(s) 256, 919
Cac8I GCNNGC 3 cut(s) 346, 363, 984
CaiI CAGNNNCTG 1 cut(s) 514
CfoI GCGC 1 cut(s) 513
Cfr13I GGNCC 5 cut(s) 241, 413, 839, 946, 965
CseI GACGC 1 cut(s) 467
Csp6I GTAC 2 cut(s) 568, 591
CviAII CATG 5 cut(s) 341, 490, 829, 942, 998
CviQI GTAC 2 cut(s) 568, 591
DdeI CTNAG 6 cut(s) 41, 381, 447, 507, 1020, 1046
DpnI GATC 5 cut(s) 26, 538, 561, 779, 1089
DpnII GATC 5 cut(s) 24, 536, 559, 777, 1087
DraIII CACNNNGTG 1 cut(s) 377
EaeI YGGCCR 1 cut(s) 315
EagI CGGCCG 1 cut(s) 315
Eam1104I CTCTTC 2 cut(s) 61, 996
EarI CTCTTC 2 cut(s) 61, 996
EciI GGCGGA 1 cut(s) 161
EclXI CGGCCG 1 cut(s) 315
Eco32I GATATC 1 cut(s) 646
Eco47I GGWCC 2 cut(s) 241, 965
Eco52I CGGCCG 1 cut(s) 315
Eco57I CTGAAG 2 cut(s) 519, 1091
Eco88I CYCGRG 1 cut(s) 915
EcoO109I RGGNCCY 1 cut(s) 241
EcoRI GAATTC 1 cut(s) 1120
EcoRII CCWGG 4 cut(s) 236, 649, 762, 814
EcoRV GATATC 1 cut(s) 646
Esp3I CGTCTC 1 cut(s) 173
FaeI CATG 5 cut(s) 344, 493, 832, 945, 1001
FalI AAGNNNNNCTT 2 cut(s) 647, 679
FaqI GGGAC 2 cut(s) 175, 227
FatI CATG 5 cut(s) 340, 489, 828, 941, 997
FbaI TGATCA 2 cut(s) 24, 559
Fnu4HI GCNGC 5 cut(s) 9, 366, 369, 1076, 1079
Fsp4HI GCNGC 5 cut(s) 9, 366, 369, 1076, 1079
FspBI CTAG 1 cut(s) 770
GlaI GCGC 1 cut(s) 512
GluI GCNGC 5 cut(s) 9, 366, 369, 1076, 1079
HaeII RGCGCY 1 cut(s) 514
HaeIII GGCC 6 cut(s) 212, 317, 353, 414, 841, 948
HapII CCGG 3 cut(s) 272, 555, 924
HgaI GACGC 1 cut(s) 467
HhaI GCGC 1 cut(s) 513
Hin1II CATG 5 cut(s) 344, 493, 832, 945, 1001
Hin6I GCGC 1 cut(s) 511
HinP1I GCGC 1 cut(s) 511
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 3 cut(s) 37, 671, 679
HpaII CCGG 3 cut(s) 272, 555, 924
HphI GGTGA 9 cut(s) 41, 102, 187, 322, 479, 514, 569, 900, 1097
Hpy166II GTNNAC 3 cut(s) 277, 712, 908
Hpy188I TCNGA 4 cut(s) 499, 547, 965, 1113
Hpy188III TCNNGA 1 cut(s) 917
Hpy8I GTNNAC 3 cut(s) 277, 712, 908
Hpy99I CGWCG 1 cut(s) 205
HpyAV CCTTC 2 cut(s) 263, 489
HpyCH4III ACNGT 1 cut(s) 297
HpyCH4V TGCA 2 cut(s) 847, 862
HpyF10VI GCNNNNNNNGC 4 cut(s) 350, 377, 794, 920
HpyF3I CTNAG 6 cut(s) 41, 381, 447, 507, 1020, 1046
Hsp92II CATG 5 cut(s) 344, 493, 832, 945, 1001
HspAI GCGC 1 cut(s) 511
KflI GGGWCCC 1 cut(s) 241
Ksp22I TGATCA 2 cut(s) 24, 559
Kzo9I GATC 5 cut(s) 24, 536, 559, 777, 1087
LmnI GCTCC 4 cut(s) 35, 80, 785, 926
Lsp1109I GCAGC 3 cut(s) 20, 352, 1087
LweI GCATC 2 cut(s) 460, 910
MaeI CTAG 1 cut(s) 770
MaeIII GTNAC 1 cut(s) 310
MalI GATC 5 cut(s) 26, 538, 561, 779, 1089
MboI GATC 5 cut(s) 24, 536, 559, 777, 1087
MboII GAAGA 8 cut(s) 48, 51, 54, 242, 634, 667, 1013, 1062
MfeI CAATTG 1 cut(s) 302
MflI RGATCY 1 cut(s) 536
MhlI GDGCHC 2 cut(s) 382, 799
MluCI AATT 5 cut(s) 120, 157, 302, 854, 1120
MlyI GAGTC 2 cut(s) 31, 673
MmeI TCCRAC 2 cut(s) 129, 943
MseI TTAA 3 cut(s) 119, 285, 825
MspA1I CMGCKG 3 cut(s) 385, 565, 1078
MspI CCGG 3 cut(s) 272, 555, 924
MspR9I CCNGG 4 cut(s) 238, 651, 764, 816
MunI CAATTG 1 cut(s) 302
MvaI CCWGG 4 cut(s) 238, 651, 764, 816
MwoI GCNNNNNNNGC 4 cut(s) 350, 377, 794, 920
NdeII GATC 5 cut(s) 24, 536, 559, 777, 1087
NlaIII CATG 5 cut(s) 344, 493, 832, 945, 1001
NlaIV GGNNCC 4 cut(s) 242, 243, 761, 947
NmuCI GTSAC 1 cut(s) 310
PaeR7I CTCGAG 1 cut(s) 915
PaqCI CACCTGC 2 cut(s) 256, 919
PasI CCCWGGG 1 cut(s) 650
PfeI GAWTC 1 cut(s) 671
PfoI TCCNGGA 1 cut(s) 762
PkrI GCNGC 5 cut(s) 10, 367, 370, 1077, 1080
PleI GAGTC 2 cut(s) 31, 673
PpsI GAGTC 2 cut(s) 31, 673
PpuMI RGGWCCY 1 cut(s) 241
Psp5II RGGWCCY 1 cut(s) 241
Psp6I CCWGG 4 cut(s) 236, 649, 762, 814
PspGI CCWGG 4 cut(s) 236, 649, 762, 814
PspN4I GGNNCC 4 cut(s) 242, 243, 761, 947
PspPI GGNCC 5 cut(s) 241, 413, 839, 946, 965
PspPPI RGGWCCY 1 cut(s) 241
PstNI CAGNNNCTG 1 cut(s) 514
PsuI RGATCY 1 cut(s) 536
PvuII CAGCTG 2 cut(s) 385, 565
RsaI GTAC 2 cut(s) 569, 592
RsaNI GTAC 2 cut(s) 568, 591
SaqAI TTAA 3 cut(s) 119, 285, 825
SatI GCNGC 5 cut(s) 9, 366, 369, 1076, 1079
Sau3AI GATC 5 cut(s) 24, 536, 559, 777, 1087
Sau96I GGNCC 5 cut(s) 241, 413, 839, 946, 965
SchI GAGTC 2 cut(s) 31, 673
ScrFI CCNGG 4 cut(s) 238, 651, 764, 816
SduI GDGCHC 2 cut(s) 382, 799
SfaNI GCATC 2 cut(s) 460, 910
SfcI CTRYAG 2 cut(s) 876, 990
Sfr274I CTCGAG 1 cut(s) 915
SinI GGWCC 2 cut(s) 241, 965
SlaI CTCGAG 1 cut(s) 915
SmlI CTYRAG 2 cut(s) 915, 1040
SmoI CTYRAG 2 cut(s) 915, 1040
Sse9I AATT 5 cut(s) 120, 157, 302, 854, 1120
SsiI CCGC 4 cut(s) 172, 216, 369, 1078
SspMI CTAG 1 cut(s) 770
StyD4I CCNGG 4 cut(s) 236, 649, 762, 814
TaaI ACNGT 1 cut(s) 297
TaqI TCGA 4 cut(s) 203, 357, 482, 916
TasI AATT 5 cut(s) 120, 157, 302, 854, 1120
TatI WGTACW 1 cut(s) 567
TauI GCSGC 2 cut(s) 371, 1081
TfiI GAWTC 1 cut(s) 671
Tru1I TTAA 3 cut(s) 119, 285, 825
Tru9I TTAA 3 cut(s) 119, 285, 825
TscAI CASTG 3 cut(s) 27, 444, 841
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 3 cut(s) 8, 365, 1075
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 2 cut(s) 17, 1014
TspRI CASTG 3 cut(s) 27, 444, 841
VpaK11BI GGWCC 2 cut(s) 241, 965
XapI RAATTY 1 cut(s) 1120
XhoI CTCGAG 1 cut(s) 915
XspI CTAG 1 cut(s) 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.