MD08G1069600.v1.1

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010419
Physical Location & Seq
Forward (+)
5513053 .. 5516945
3893 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1069600.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGAAAGCAGCACTGTCAACACTAATCAGCTCCAAGAATCTCAACCCTCACTCCTCCTCCTCCTCTTCTCTCTGCCGCTCCATTTGCCTCCGCCCGAACCCTCTCTCCCCTCCGCCGTATTTCCTCTCTCCAATCGCACCCTCCAAAGCCCTCACCACCAACATCCGCATCGGTCTCAGCCGCAAGTCCCAGACCCGCTCTGCAAATTTCTCGTCGGTAATGGCGTCGGTTTACAAGCCCGAAGAAGCCAGGGTCCCGCCTGCCCTTCCCCTGCCCACTCCTCCCGTCACCAAGGCAAGCTTTCTCTTTAATCTCTGTGTGTATCCGTTTAAGATTGGGCTGTGTCAGTTGTCTGTGACGCCGGATAAGGAGAGGAACATTGCCCATGCTCGCAAGGCCATCGAGGACGCCGCCGCAAAGGGTGCCCAGCTTGTTCTTCTACCTGAAATTTGGAATGGTCCATATTCAAATGACAGCTTCCCGGTTTATGCTGAGGACATTGATGCAGGCGGCGATGCATCTCCTTCAACAGCCATGCTGTCTGAAGTTGCTCAACGTTTGAAGATCACCATTGTTGGTGGATCTATAGCGGAGCGTTCTGGGGTTCGGTTGTACAATACTTCTTGTGTCTTTGGCACCGATGGAAAGCTGTTAGCTAAACACAGGAAGATACACCTATTTGATATCGACATCCCGGGGAAGATTACCTTCATTGAATCAAAGACCCTCACAGCAGGGCAGACTCCTACCATTGTGGATACAGAAGTTGGACGTATTGGCATTGGTATCTGTTATGACATTCGGTTTCCGGAACTAGCAATGATTTATGGAGCAAGAGGTGCTCACTTGATATGCTATCCTGGGGCTTTTAACATGACTACTGGACCGTTGCACTGGGAATTATTGCAGAGGGCAAGGGCTACAGATAACCAGCTGTATGTAGCAACTTGTTCTCCTGCTCGGGATGCTGGAGCCGGTTATGTGGCATGGGGCCACTCCACCCTTGTTGGACCATTTGGAGAAGTACTGGCGACAACAGAACATGAAGAGGCAATAATCATAGCAGAGATTGATTATTCAATTCTTGAGCTTAGAAGGACAAACCTTCCCTTACTGAAGCAAAGACGAGGCGATCTTTACCAATTGGTAGATGTTCAGAGGTTAGATTCTCAATGA

Protein Analysis

392

Amino Acids

42.49

Weight (kDa)

8.69

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 111 - 360 8.4e-64 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 422, 635
AccBSI CCGCTC 2 cut(s) 78, 198
AccIII TCCGGA 1 cut(s) 808
AclI AACGTT 1 cut(s) 556
AclWI GGATC 1 cut(s) 589
AcsI RAATTY 2 cut(s) 205, 447
AcuI CTGAAG 2 cut(s) 564, 1136
AcyI GRCGYC 3 cut(s) 224, 359, 408
AfaI GTAC 2 cut(s) 614, 1026
AgsI TTSAA 5 cut(s) 468, 528, 562, 716, 1080
AjnI CCWGG 2 cut(s) 248, 859
AloI GAACNNNNNNTCC 2 cut(s) 89, 121
AluBI AGCT 8 cut(s) 30, 300, 430, 477, 649, 656, 934, 1090
AluI AGCT 8 cut(s) 30, 300, 430, 477, 649, 656, 934, 1090
Alw21I GWGCWC 1 cut(s) 844
Alw26I GTCTC 1 cut(s) 179
AlwI GGATC 1 cut(s) 589
Ama87I CYCGRG 2 cut(s) 694, 960
Aor13HI TCCGGA 1 cut(s) 808
AoxI GGCC 2 cut(s) 396, 991
ApeKI GCWGC 1 cut(s) 8
ApoI RAATTY 2 cut(s) 205, 447
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
AspS9I GGNCC 5 cut(s) 253, 458, 884, 991, 1010
AsuC2I CCSGG 3 cut(s) 482, 695, 696
AsuHPI GGTGA 3 cut(s) 145, 280, 559
AvaI CYCGRG 2 cut(s) 694, 960
AvaII GGWCC 4 cut(s) 253, 458, 884, 1010
BaeGI GKGCMC 1 cut(s) 427
BaeI ACNNNNGTAYC 2 cut(s) 750, 783
BanI GGYRCC 2 cut(s) 422, 635
Bbv12I GWGCWC 1 cut(s) 844
BbvCI CCTCAGC 1 cut(s) 492
BbvI GCAGC 1 cut(s) 20
BccI CCATC 2 cut(s) 407, 635
BceAI ACGGC 1 cut(s) 100
BcgI CGANNNNNNTGC 4 cut(s) 192, 226, 382, 416
BciT130I CCWGG 2 cut(s) 250, 861
BciVI GTATCC 2 cut(s) 333, 751
BcnI CCSGG 3 cut(s) 482, 695, 696
BcoDI GTCTC 1 cut(s) 179
BfaI CTAG 1 cut(s) 815
BfmI CTRYAG 2 cut(s) 585, 921
BfuI GTATCC 2 cut(s) 333, 751
BisI GCNGC 6 cut(s) 9, 76, 181, 411, 414, 511
BlsI GCNGC 6 cut(s) 10, 77, 182, 412, 415, 512
BmcAI AGTACT 1 cut(s) 1026
Bme1390I CCNGG 5 cut(s) 250, 482, 695, 696, 861
Bme18I GGWCC 4 cut(s) 253, 458, 884, 1010
BmeT110I CYCGRG 2 cut(s) 694, 960
BmgT120I GGNCC 5 cut(s) 253, 458, 884, 991, 1010
BmiI GGNNCC 6 cut(s) 254, 255, 424, 637, 973, 992
BmrFI CCNGG 5 cut(s) 250, 482, 695, 696, 861
BmrI ACTGGG 1 cut(s) 904
BmsI GCATC 5 cut(s) 177, 493, 505, 527, 955
BmuI ACTGGG 1 cut(s) 904
BpmI CTGGAG 1 cut(s) 990
Bpu10I CCTNAGC 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 1106
BpuMI CCSGG 3 cut(s) 482, 695, 696
BsaHI GRCGYC 3 cut(s) 224, 359, 408
BsaI GGTCTC 1 cut(s) 179
BsaJI CCNNGG 5 cut(s) 249, 291, 694, 695, 860
BsaWI WCCGGW 1 cut(s) 808
BsaXI ACNNNNNCTCC 8 cut(s) 35, 41, 65, 71, 89, 119, 937, 967
Bse118I RCCGGY 1 cut(s) 974
Bse1I ACTGG 3 cut(s) 886, 899, 1032
Bse3DI GCAATG 2 cut(s) 378, 825
BseAI TCCGGA 1 cut(s) 808
BseBI CCWGG 2 cut(s) 250, 861
BseDI CCNNGG 5 cut(s) 249, 291, 694, 695, 860
BseGI GGATG 3 cut(s) 162, 690, 970
BseMI GCAATG 2 cut(s) 378, 825
BseMII CTCAG 2 cut(s) 190, 483
BseNI ACTGG 3 cut(s) 886, 899, 1032
BseRI GAGGAG 5 cut(s) 43, 46, 49, 52, 270
BseSI GKGCMC 1 cut(s) 427
BseXI GCAGC 1 cut(s) 20
BseYI CCCAGC 1 cut(s) 426
BshFI GGCC 2 cut(s) 398, 993
BshNI GGYRCC 2 cut(s) 422, 635
BsiHKAI GWGCWC 1 cut(s) 844
BsiHKCI CYCGRG 2 cut(s) 694, 960
BsiSI CCGG 5 cut(s) 362, 482, 695, 809, 975
BslFI GGGAC 2 cut(s) 172, 239
BsmAI GTCTC 1 cut(s) 179
BsmFI GGGAC 2 cut(s) 172, 239
BsnI GGCC 2 cut(s) 398, 993
Bso31I GGTCTC 1 cut(s) 179
BsoBI CYCGRG 2 cut(s) 694, 960
Bsp1286I GDGCHC 2 cut(s) 427, 844
Bsp13I TCCGGA 1 cut(s) 808
Bsp1407I TGTACA 1 cut(s) 612
Bsp143I GATC 3 cut(s) 564, 581, 1132
BspANI GGCC 2 cut(s) 398, 993
BspCNI CTCAG 2 cut(s) 189, 484
BspEI TCCGGA 1 cut(s) 808
BspLI GGNNCC 6 cut(s) 254, 255, 424, 637, 973, 992
BspPI GGATC 1 cut(s) 589
BspT107I GGYRCC 2 cut(s) 422, 635
BspTNI GGTCTC 1 cut(s) 179
BsrBI CCGCTC 2 cut(s) 78, 198
BsrDI GCAATG 2 cut(s) 378, 825
BsrFI RCCGGY 1 cut(s) 974
BsrGI TGTACA 1 cut(s) 612
BsrI ACTGG 3 cut(s) 886, 899, 1032
BssAI RCCGGY 1 cut(s) 974
BssECI CCNNGG 5 cut(s) 249, 291, 694, 695, 860
BssMI GATC 3 cut(s) 564, 581, 1132
BssNI GRCGYC 3 cut(s) 224, 359, 408
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 2 cut(s) 250, 861
Bst4CI ACNGT 2 cut(s) 15, 888
Bst6I CTCTTC 2 cut(s) 70, 1041
BstACI GRCGYC 3 cut(s) 224, 359, 408
BstAPI GCANNNNNTGC 2 cut(s) 422, 839
BstAUI TGTACA 1 cut(s) 612
BstC8I GCNNGC 4 cut(s) 261, 298, 391, 508
BstDEI CTNAG 3 cut(s) 176, 492, 1091
BstF5I GGATG 3 cut(s) 162, 690, 970
BstKTI GATC 3 cut(s) 567, 584, 1135
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 3 cut(s) 564, 581, 1132
BstMWI GCNNNNNNNGC 5 cut(s) 84, 395, 422, 839, 965
BstNI CCWGG 2 cut(s) 250, 861
BstSCI CCNGG 5 cut(s) 248, 480, 693, 694, 859
BstSFI CTRYAG 2 cut(s) 585, 921
BstSLI GKGCMC 1 cut(s) 427
BstV1I GCAGC 1 cut(s) 20
BstX2I RGATCY 1 cut(s) 581
BstYI RGATCY 1 cut(s) 581
BsuI GTATCC 2 cut(s) 333, 751
BsuRI GGCC 2 cut(s) 398, 993
BtgZI GCGATG 1 cut(s) 528
BtsCI GGATG 3 cut(s) 162, 690, 970
BtsIMutI CAGTG 2 cut(s) 11, 892
Cac8I GCNNGC 4 cut(s) 261, 298, 391, 508
Cfr10I RCCGGY 1 cut(s) 974
Cfr13I GGNCC 5 cut(s) 253, 458, 884, 991, 1010
Cfr9I CCCGGG 1 cut(s) 694
CseI GACGC 3 cut(s) 213, 367, 416
Csp6I GTAC 2 cut(s) 613, 1025
CspCI CAANNNNNGTGG 2 cut(s) 988, 1023
CviAII CATG 5 cut(s) 386, 535, 874, 987, 1043
CviQI GTAC 2 cut(s) 613, 1025
DdeI CTNAG 3 cut(s) 176, 492, 1091
DpnI GATC 3 cut(s) 566, 583, 1134
DpnII GATC 3 cut(s) 564, 581, 1132
Eam1104I CTCTTC 2 cut(s) 70, 1041
EarI CTCTTC 2 cut(s) 70, 1041
EciI GGCGGA 2 cut(s) 80, 102
Eco130I CCWWGG 1 cut(s) 291
Eco31I GGTCTC 1 cut(s) 179
Eco32I GATATC 1 cut(s) 685
Eco47I GGWCC 4 cut(s) 253, 458, 884, 1010
Eco57I CTGAAG 2 cut(s) 564, 1136
Eco88I CYCGRG 2 cut(s) 694, 960
EcoO109I RGGNCCY 1 cut(s) 253
EcoRII CCWGG 2 cut(s) 248, 859
EcoRV GATATC 1 cut(s) 685
EcoT14I CCWWGG 1 cut(s) 291
EcoT22I ATGCAT 1 cut(s) 520
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 5 cut(s) 389, 538, 877, 990, 1046
FalI AAGNNNNNCTT 4 cut(s) 284, 316, 692, 724
FaqI GGGAC 2 cut(s) 172, 239
FatI CATG 5 cut(s) 385, 534, 873, 986, 1042
FauI CCCGC 2 cut(s) 203, 264
Fnu4HI GCNGC 6 cut(s) 9, 76, 181, 411, 414, 511
FokI GGATG 3 cut(s) 149, 677, 977
Fsp4HI GCNGC 6 cut(s) 9, 76, 181, 411, 414, 511
FspBI CTAG 1 cut(s) 815
GluI GCNGC 6 cut(s) 9, 76, 181, 411, 414, 511
GsaI CCCAGC 1 cut(s) 430
GsuI CTGGAG 1 cut(s) 990
HaeIII GGCC 2 cut(s) 398, 993
HapII CCGG 5 cut(s) 362, 482, 695, 809, 975
HgaI GACGC 3 cut(s) 213, 367, 416
Hin1I GRCGYC 3 cut(s) 224, 359, 408
Hin1II CATG 5 cut(s) 389, 538, 877, 990, 1046
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HindIII AAGCTT 1 cut(s) 298
HinfI GANTC 4 cut(s) 37, 716, 742, 1166
HpaII CCGG 5 cut(s) 362, 482, 695, 809, 975
HphI GGTGA 3 cut(s) 145, 280, 559
Hpy166II GTNNAC 2 cut(s) 18, 232
Hpy188I TCNGA 2 cut(s) 544, 1158
Hpy188III TCNNGA 3 cut(s) 809, 962, 1085
Hpy8I GTNNAC 2 cut(s) 18, 232
Hpy99I CGWCG 2 cut(s) 217, 229
HpyAV CCTTC 5 cut(s) 275, 534, 718, 1089, 1115
HpyCH4III ACNGT 2 cut(s) 15, 888
HpyCH4IV ACGT 2 cut(s) 556, 772
HpyCH4V TGCA 5 cut(s) 203, 506, 518, 892, 907
HpyF10VI GCNNNNNNNGC 5 cut(s) 84, 395, 422, 839, 965
HpyF3I CTNAG 3 cut(s) 176, 492, 1091
HpySE526I ACGT 2 cut(s) 556, 772
Hsp92I GRCGYC 3 cut(s) 224, 359, 408
Hsp92II CATG 5 cut(s) 389, 538, 877, 990, 1046
KflI GGGWCCC 1 cut(s) 253
Kpn2I TCCGGA 1 cut(s) 808
Kzo9I GATC 3 cut(s) 564, 581, 1132
LmnI GCTCC 5 cut(s) 35, 83, 592, 830, 971
Lsp1109I GCAGC 1 cut(s) 20
LweI GCATC 5 cut(s) 177, 493, 505, 527, 955
MaeI CTAG 1 cut(s) 815
MaeII ACGT 2 cut(s) 556, 772
MaeIII GTNAC 2 cut(s) 286, 355
MalI GATC 3 cut(s) 566, 583, 1134
MbiI CCGCTC 2 cut(s) 78, 198
MboI GATC 3 cut(s) 564, 581, 1132
MboII GAAGA 7 cut(s) 57, 254, 428, 574, 679, 712, 1058
MfeI CAATTG 1 cut(s) 1142
MflI RGATCY 1 cut(s) 581
MhlI GDGCHC 2 cut(s) 427, 844
MluCI AATT 5 cut(s) 205, 447, 899, 1080, 1142
MlyI GAGTC 1 cut(s) 736
MmeI TCCRAC 2 cut(s) 748, 988
Mph1103I ATGCAT 1 cut(s) 520
MroI TCCGGA 1 cut(s) 808
MseI TTAA 3 cut(s) 309, 330, 870
MspA1I CMGCKG 1 cut(s) 934
MspI CCGG 5 cut(s) 362, 482, 695, 809, 975
MspR9I CCNGG 5 cut(s) 250, 482, 695, 696, 861
MunI CAATTG 1 cut(s) 1142
MvaI CCWGG 2 cut(s) 250, 861
MwoI GCNNNNNNNGC 5 cut(s) 84, 395, 422, 839, 965
NciI CCSGG 3 cut(s) 482, 695, 696
NdeII GATC 3 cut(s) 564, 581, 1132
NlaIII CATG 5 cut(s) 389, 538, 877, 990, 1046
NlaIV GGNNCC 6 cut(s) 254, 255, 424, 637, 973, 992
NmuCI GTSAC 2 cut(s) 286, 355
NsiI ATGCAT 1 cut(s) 520
PcsI WCGNNNNNNNCGW 1 cut(s) 221
PfeI GAWTC 3 cut(s) 37, 716, 1166
PkrI GCNGC 6 cut(s) 10, 77, 182, 412, 415, 512
PleI GAGTC 1 cut(s) 736
PpsI GAGTC 1 cut(s) 736
PpuMI RGGWCCY 1 cut(s) 253
Psp1406I AACGTT 1 cut(s) 556
Psp5II RGGWCCY 1 cut(s) 253
Psp6I CCWGG 2 cut(s) 248, 859
PspFI CCCAGC 1 cut(s) 426
PspGI CCWGG 2 cut(s) 248, 859
PspN4I GGNNCC 6 cut(s) 254, 255, 424, 637, 973, 992
PspPI GGNCC 5 cut(s) 253, 458, 884, 991, 1010
PspPPI RGGWCCY 1 cut(s) 253
PsuI RGATCY 1 cut(s) 581
PvuII CAGCTG 1 cut(s) 934
RsaI GTAC 2 cut(s) 614, 1026
RsaNI GTAC 2 cut(s) 613, 1025
SaqAI TTAA 3 cut(s) 309, 330, 870
SatI GCNGC 6 cut(s) 9, 76, 181, 411, 414, 511
Sau3AI GATC 3 cut(s) 564, 581, 1132
Sau96I GGNCC 5 cut(s) 253, 458, 884, 991, 1010
ScaI AGTACT 1 cut(s) 1026
SchI GAGTC 1 cut(s) 736
ScrFI CCNGG 5 cut(s) 250, 482, 695, 696, 861
SduI GDGCHC 2 cut(s) 427, 844
SfaNI GCATC 5 cut(s) 177, 493, 505, 527, 955
SfcI CTRYAG 2 cut(s) 585, 921
SinI GGWCC 4 cut(s) 253, 458, 884, 1010
SmaI CCCGGG 1 cut(s) 696
SmlI CTYRAG 1 cut(s) 1085
SmoI CTYRAG 1 cut(s) 1085
Sse9I AATT 5 cut(s) 205, 447, 899, 1080, 1142
SspMI CTAG 1 cut(s) 815
StyD4I CCNGG 5 cut(s) 248, 480, 693, 694, 859
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 2 cut(s) 15, 888
TaiI ACGT 2 cut(s) 559, 775
TaqI TCGA 2 cut(s) 402, 687
TaqII GACCGA 1 cut(s) 161
TasI AATT 5 cut(s) 205, 447, 899, 1080, 1142
TatI WGTACW 2 cut(s) 612, 1024
TauI GCSGC 5 cut(s) 78, 183, 413, 416, 513
TfiI GAWTC 3 cut(s) 37, 716, 1166
Tru1I TTAA 3 cut(s) 309, 330, 870
Tru9I TTAA 3 cut(s) 309, 330, 870
TscAI CASTG 2 cut(s) 18, 899
TseFI GTSAC 2 cut(s) 286, 355
TseI GCWGC 1 cut(s) 8
Tsp45I GTSAC 2 cut(s) 286, 355
TspDTI ATGAA 3 cut(s) 17, 700, 1059
TspGWI ACGGA 1 cut(s) 315
TspMI CCCGGG 1 cut(s) 694
TspRI CASTG 2 cut(s) 18, 899
VpaK11BI GGWCC 4 cut(s) 253, 458, 884, 1010
XapI RAATTY 2 cut(s) 205, 447
XmaI CCCGGG 1 cut(s) 694
XspI CTAG 1 cut(s) 815
ZrmI AGTACT 1 cut(s) 1026
Zsp2I ATGCAT 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.