Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0008873

Physical Location & Seq

Genomic Coordinates Reverse (-)

53374126 .. 53379054

Gene Structure 4929 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0389400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2499 bp

ATGGGACACTTTATGCAACAATTTATGGGATTGATAAGCTACACAGTGGATGTGGACTCGGCTTATTGTGGAAGTGTTCTTGCCATAGTAACTTGCTTTTTATTTCTTAATTACTTTCACATTCTGGATGCACAACAGTCGCCTAAAAAATGTGTAGATCAAATCAAGAGGCTGGTACTGTGCGGACCTCAGATCTCTGGTTCCAAAATCTATGCAACAGTTGATCTAGACAAGGCTCGGGTGGCACGAACCCGAATGATAAACAATCCCCACAACCCCATATGGAAGGAAGATTTCCACATCTACAGTGCTCATTTCATCTCACACATTATCTTCACTGTCAAAGCTAATGATCCTATTGGTGCCACACTCATAGGAAGAGCTTATATACCAGTTGAAGATATCATAAAGGGCTATGTGGTGGACAGATGGGTTGAGATACTAGATGAAGAACACAATCCCATCCATGGAAACTCTAGAATTCATGTCAAGTTGCAATTCTCAAGTGTTGCAGAAGATGCTCATTGCCATTGGTCTCAGGGACTCAGAAGTCCTGACTATGAGGGTGTTCCTTGGACCTTTTTCAATCAAAGACAGGGTTGCAGGGTTACCCTCTACCAAGATTCGCATGTCGCGGATGATTTTAATCCTGGGATTCCTATGGCCAATGGGGGATTCTACGAGCCACGAGGATGTTGGGAGGACATATTTGATGCAATCACTAATGCAAAACACTTGATCTACATAACTGGCTGGTCTGTCTCCACTGAAATAAACTTGCTGAGGGATCCACAAAGGCGAAAAGCAGGAGATGAGCTCACATTAGGACAGCTTCTCAAGAAAAAGGCTGATGAGGGTGTTCTAGTTCTCTTGCTTGTTTGGGATGACAGAACTTCTATTCCTCAATTTAAAAAGGATGGTCTGATGACAACACATGATCAAGAAACTGAGGAGTACTTTCGAAACACAAAAGTTCATTGCTTCCTTTGCCCGAGGAACCCTGATCTTGGTAGAAGCATTATTCAAGGTTTTGAGACTTCTACAATGTTCACTCACCATCAAAAAACAGTTGTAGTTGACAGTGAGATAGTCAGTGGAGATCCCTCGCAAAAAAGGAGGATTCTGAGCTTTGTTGGAGGCATTGATCTTTGTGATGGAAGATATGACACTCAGAAACACCCCCTATTTTCAACTCTAGGCACAACTCACCATAAGGACTTCCATCAGCCAAACTTTCCAGGCTCTTCGATTAGAAAAGGCGGTCCAAGAGAGCCATGGCATGATATCCATTGCAAACTTGAAGGGCCTGTTGCTTGGGATGTCTTGTACAACTTTGAGCAGAGGTGGAAAAAGCAAGTTGGTGACAAGTTCTTATTTTCACTAAGCAAGCTTGAGGAAATCACAGTTCACCCTTTACAGAACAAGTTATCAAATGACAGTGAAGCATGGAATGTTCAGCTGTTTCGTTCCATCGACAATGGAGCTGCTTTGGGATTTCCTGAGAATCCACTTGAAGCAACACAAAGGGGACTTGTGAGTGGGAAGAACAACATCATAGACAGAAGCATCCAGGATGCATATGTCCATGCCATTCGCCGAGCAAAGAATTTCATTTACATAGAGAATCAGTACTTCCTTGGAAGCTCATTTGGGTGGAGATCAAAAGATATCAAGGATGAGGATATTAATGCTCTGCATCTTATCCCAAAGGAGCTGTCACTGAAGATTGTTAGTAAAATTGAAGCAGGGGAGAGGTTTGCTGTCTACATTGTTCTCCCAATGTGGCCAGAAGGTATACCCGAGAGTGGTTCTGTTCAGGCTATATTAGATTGGCAAAAAAGGACATTGGAGATGATGTACACTGATATCTCTGAGGCCCTTCAGAGAAAGGGTTTGAATGCAAACCCTAGAGACTATCTTACATTCTTCTGCCTTGGGAACCGGGAGACCGTGAAGGATGGAGAATATGTACCTCCAGAGACTCCAGAGCCAGACACTGATTACATCAGAGCTCAGAAGGCCCGGCGCTTCATGATTTATGTGCACTCAAAAATGATGATAGTTGATGATGAATACATTATCATTGGATCTGCCAACATAAACCAAAGGTCAATGGATGGGGCGAGGGACTCCGAAATTGCTATGGGAGCATTTCAGACTAATTATTTGGCTAATTCCACTGACCCAGCTAGGGGAGAGATATATGCTTTCAGGGTGACACTATGGTATGAGCACCTTAAAGTGCTTGACAGCTCTTTCTTGAATCCAGAATCAATGGAATGCATCCGGACGGTGAATCACCTTGCGGAAACAAACTGGGATCTTTTCTCAAGTGACACTTTTCCTGGTGACCTTCCCGGTCACCTTCTTCGATATCCTGTTGAGGTAAGCAAGACTGGAGCTCTAACAACACTGCCGGGGTTCGAACATTTCCCCGACACCAAGGCTCGAGTCTTTGGCACCAAGTCTGAGTACCTTCTTCCACTCCTTACCACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

832

Amino Acids

94.96

Weight (kDa)

6.0

Isoelectric Point (pI)

37.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	65 - 146	1e-09	C2 domain
PLDc	PF00614	679 - 704	3.5e-08	Phospholipase D Active site motif
PLDc_2	PF13091	679 - 710	4.1e-06	PLD-like domain
PLD_C	PF12357	750 - 822	1.4e-27	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008873)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g18940 FvH4_6g18941
malus_domestica	MD08G1069200.v1.1 MD08G1069300.v1.1 MD15G1052800.v1.1
prunus_persica	Prupe.1G406700_v2.0.a1
pyrus_communis	pycom08g05700 pycom08g05710 pycom15g05050
rosa_chinensis	RchiOBHm_Chr6g0311521
rosa_laevigata	RLG00000010397
rosa_multiflora	Rmu_co8085934.1_g000001 Rmu_sc0028020.1_g000001 Rmu_sc0035116.1_g000001
rosa_roxburghii	Rroxscaffold_7G00157540
rosa_rugosa	Rorug06G0389400
rosa_samantha	Rh6AG504200 Rh6AG504300 Rh6BG514900 Rh6CG520700 Rh6DG506700 Rh7DG318900
rosa_wichuraiana	Rw6G043910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	549
AccB1I	GGYRCC	2 cut(s)	362, 2459
AccI	GTMKAC	2 cut(s)	1764, 1795
AccII	CGCG	1 cut(s)	635
AccIII	TCCGGA	1 cut(s)	2286
AciI	CCGC	4 cut(s)	183, 635, 1260, 2306
AclWI	GGATC	6 cut(s)	347, 782, 795, 1094, 2095, 2328
AcoI	YGGCCR	2 cut(s)	663, 1784
AcsI	RAATTY	2 cut(s)	480, 1606
AcuI	CTGAAG	2 cut(s)	1742, 1865
AfaI	GTAC	7 cut(s)	177, 956, 1328, 1631, 1859, 1971, 2474
AfiI	CCNNNNNNNGG	5 cut(s)	467, 1007, 1805, 2191, 2192
AgsI	TTSAA	9 cut(s)	398, 586, 1025, 1191, 1301, 1514, 1742, 1897, 2263
AjnI	CCWGG	4 cut(s)	649, 1237, 1569, 2344
AjuI	GAANNNNNNNTTGG	2 cut(s)	659, 691
AloI	GAACNNNNNNTCC	2 cut(s)	883, 915
Alw21I	GWGCWC	6 cut(s)	313, 819, 2014, 2046, 2235, 2404
Alw26I	GTCTC	6 cut(s)	540, 766, 1028, 1905, 1940, 1973
Alw44I	GTGCAC	1 cut(s)	2042
AlwI	GGATC	6 cut(s)	347, 782, 795, 1094, 2095, 2328
AlwNI	CAGNNNCTG	1 cut(s)	1997
Ama87I	CYCGRG	4 cut(s)	237, 991, 1799, 2448
Aor13HI	TCCGGA	1 cut(s)	2286
AoxI	GGCC	5 cut(s)	663, 1304, 1784, 1875, 2019
ApaLI	GTGCAC	1 cut(s)	2042
ApeKI	GCWGC	1 cut(s)	1484
ApoI	RAATTY	2 cut(s)	480, 1606
ArsI	GACNNNNNNTTYG	4 cut(s)	1700, 1732, 2149, 2181
AseI	ATTAAT	1 cut(s)	1686
AspLEI	GCGC	1 cut(s)	2028
AspS9I	GGNCC	6 cut(s)	185, 576, 1262, 1304, 1876, 2020
AsuC2I	CCSGG	4 cut(s)	1943, 2023, 2358, 2418
AsuHPI	GGTGA	9 cut(s)	1046, 1199, 1373, 1400, 2227, 2291, 2305, 2354, 2360
AsuII	TTCGAA	2 cut(s)	961, 2424
AvaI	CYCGRG	4 cut(s)	237, 991, 1799, 2448
AvaII	GGWCC	3 cut(s)	185, 576, 1262
BaeGI	GKGCMC	1 cut(s)	2046
BalI	TGGCCA	2 cut(s)	665, 1786
BamHI	GGATCC	1 cut(s)	787
BanI	GGYRCC	2 cut(s)	362, 2459
BanII	GRGCYC	3 cut(s)	819, 2014, 2404
BauI	CACGAG	1 cut(s)	687
Bbv12I	GWGCWC	6 cut(s)	313, 819, 2014, 2046, 2235, 2404
BbvCI	CCTCAGC	1 cut(s)	782
BbvI	GCAGC	1 cut(s)	1471
BccI	CCATC	9 cut(s)	423, 470, 911, 1065, 1148, 1230, 1478, 1952, 2111
BcgI	CGANNNNNNTGC	2 cut(s)	120, 154
BciT130I	CCWGG	4 cut(s)	651, 1239, 1571, 2346
BclI	TGATCA	1 cut(s)	937
BcnI	CCSGG	4 cut(s)	1943, 2023, 2358, 2418
BcoDI	GTCTC	6 cut(s)	540, 766, 1028, 1905, 1940, 1973
BfaI	CTAG	8 cut(s)	227, 443, 477, 863, 1196, 1908, 2190, 2497
BfmI	CTRYAG	1 cut(s)	304
BfoI	RGCGCY	1 cut(s)	2029
BglII	AGATCT	1 cut(s)	192
BisI	GCNGC	1 cut(s)	1485
BlsI	GCNGC	1 cut(s)	1486
BmcAI	AGTACT	2 cut(s)	956, 1631
Bme1390I	CCNGG	8 cut(s)	651, 1239, 1571, 1943, 2023, 2346, 2358, 2418
Bme18I	GGWCC	3 cut(s)	185, 576, 1262
BmeT110I	CYCGRG	4 cut(s)	237, 991, 1799, 2448
BmgT120I	GGNCC	6 cut(s)	185, 576, 1262, 1304, 1876, 2020
BmiI	GGNNCC	6 cut(s)	202, 364, 789, 998, 1940, 2461
BmrFI	CCNGG	8 cut(s)	651, 1239, 1571, 1943, 2023, 2346, 2358, 2418
BmrI	ACTGGG	1 cut(s)	2326
BmsI	GCATC	7 cut(s)	118, 508, 703, 1564, 1575, 1705, 2292
BmuI	ACTGGG	1 cut(s)	2326
BplI	GAGNNNNNCTC	2 cut(s)	801, 833
BpmI	CTGGAG	3 cut(s)	1959, 1968, 2418
Bpu10I	CCTNAGC	1 cut(s)	782
Bpu14I	TTCGAA	2 cut(s)	961, 2424
BpuEI	CTTGAG	4 cut(s)	487, 821, 1412, 2314
BpuMI	CCSGG	4 cut(s)	1943, 2023, 2358, 2418
BsaBI	GATNNNNATC	1 cut(s)	645
BsaI	GGTCTC	2 cut(s)	540, 1940
BsaJI	CCNNGG	9 cut(s)	466, 572, 650, 992, 1274, 1636, 1933, 2417, 2442
BsaWI	WCCGGW	1 cut(s)	2286
BsaXI	ACNNNNNCTCC	4 cut(s)	1841, 1871, 1953, 1983
Bsc4I	CCNNNNNNNGG	5 cut(s)	467, 1007, 1805, 2191, 2192
Bse1I	ACTGG	4 cut(s)	392, 754, 2321, 2401
Bse3DI	GCAATG	3 cut(s)	523, 976, 1288
Bse8I	GATNNNNATC	1 cut(s)	645
BseAI	TCCGGA	1 cut(s)	2286
BseBI	CCWGG	4 cut(s)	651, 1239, 1571, 2346
BseDI	CCNNGG	9 cut(s)	466, 572, 650, 992, 1274, 1636, 1933, 2417, 2442
BseJI	GATNNNNATC	1 cut(s)	645
BseLI	CCNNNNNNNGG	5 cut(s)	467, 1007, 1805, 2191, 2192
BseMI	GCAATG	3 cut(s)	523, 976, 1288
BseNI	ACTGG	4 cut(s)	392, 754, 2321, 2401
BseRI	GAGGAG	1 cut(s)	965
BseSI	GKGCMC	1 cut(s)	2046
BseXI	GCAGC	1 cut(s)	1471
BseYI	CCCAGC	1 cut(s)	2185
Bsh1236I	CGCG	1 cut(s)	635
BshFI	GGCC	5 cut(s)	665, 1306, 1786, 1877, 2021
BshNI	GGYRCC	2 cut(s)	362, 2459
BsiHKAI	GWGCWC	6 cut(s)	313, 819, 2014, 2046, 2235, 2404
BsiHKCI	CYCGRG	4 cut(s)	237, 991, 1799, 2448
BsiSI	CCGG	5 cut(s)	1942, 2023, 2287, 2358, 2417
BslFI	GGGAC	4 cut(s)	18, 555, 1542, 2141
BslI	CCNNNNNNNGG	5 cut(s)	467, 1007, 1805, 2191, 2192
BsmAI	GTCTC	6 cut(s)	540, 766, 1028, 1905, 1940, 1973
BsmFI	GGGAC	4 cut(s)	18, 555, 1542, 2141
BsmI	GAATGC	2 cut(s)	1903, 2285
BsnI	GGCC	5 cut(s)	665, 1306, 1786, 1877, 2021
Bso31I	GGTCTC	2 cut(s)	540, 1940
BsoBI	CYCGRG	4 cut(s)	237, 991, 1799, 2448
Bsp119I	TTCGAA	2 cut(s)	961, 2424
Bsp1286I	GDGCHC	6 cut(s)	313, 819, 2014, 2046, 2235, 2404
Bsp13I	TCCGGA	1 cut(s)	2286
Bsp1407I	TGTACA	2 cut(s)	1326, 1857
Bsp19I	CCATGG	2 cut(s)	466, 1274
BspACI	CCGC	4 cut(s)	183, 635, 1260, 2306
BspANI	GGCC	5 cut(s)	665, 1306, 1786, 1877, 2021
BspEI	TCCGGA	1 cut(s)	2286
BspFNI	CGCG	1 cut(s)	635
BspHI	TCATGA	1 cut(s)	2031
BspLI	GGNNCC	6 cut(s)	202, 364, 789, 998, 1940, 2461
BspPI	GGATC	6 cut(s)	347, 782, 795, 1094, 2095, 2328
BspQI	GCTCTTC	2 cut(s)	373, 1249
BspT104I	TTCGAA	2 cut(s)	961, 2424
BspT107I	GGYRCC	2 cut(s)	362, 2459
BspTNI	GGTCTC	2 cut(s)	540, 1940
BsrDI	GCAATG	3 cut(s)	523, 976, 1288
BsrGI	TGTACA	2 cut(s)	1326, 1857
BsrI	ACTGG	4 cut(s)	392, 754, 2321, 2401
BssECI	CCNNGG	9 cut(s)	466, 572, 650, 992, 1274, 1636, 1933, 2417, 2442
BssNAI	GTATAC	1 cut(s)	1796
BssSI	CACGAG	1 cut(s)	687
BssT1I	CCWWGG	6 cut(s)	466, 572, 1274, 1636, 1933, 2442
Bst1107I	GTATAC	1 cut(s)	1796
Bst2BI	CACGAG	1 cut(s)	687
Bst2UI	CCWGG	4 cut(s)	651, 1239, 1571, 2346
Bst6I	CTCTTC	2 cut(s)	373, 1249
BstAPI	GCANNNNNTGC	1 cut(s)	518
BstAUI	TGTACA	2 cut(s)	1326, 1857
BstBI	TTCGAA	2 cut(s)	961, 2424
BstC8I	GCNNGC	1 cut(s)	1388
BstDSI	CCRYGG	2 cut(s)	466, 1274
BstEII	GGTNACC	3 cut(s)	607, 2348, 2360
BstFNI	CGCG	1 cut(s)	635
BstH2I	RGCGCY	1 cut(s)	2029
BstHHI	GCGC	1 cut(s)	2028
BstMAI	GTCTC	6 cut(s)	540, 766, 1028, 1905, 1940, 1973
BstMWI	GCNNNNNNNGC	5 cut(s)	242, 518, 987, 2018, 2147
BstNI	CCWGG	4 cut(s)	651, 1239, 1571, 2346
BstNSI	RCATGY	1 cut(s)	632
BstPI	GGTNACC	3 cut(s)	607, 2348, 2360
BstSCI	CCNGG	8 cut(s)	649, 1237, 1569, 1941, 2021, 2344, 2356, 2416
BstSFI	CTRYAG	1 cut(s)	304
BstSLI	GKGCMC	1 cut(s)	2046
BstUI	CGCG	1 cut(s)	635
BstV1I	GCAGC	1 cut(s)	1471
BstX2I	RGATCY	5 cut(s)	192, 787, 1099, 2087, 2320
BstYI	RGATCY	5 cut(s)	192, 787, 1099, 2087, 2320
BstZ17I	GTATAC	1 cut(s)	1796
BsuRI	GGCC	5 cut(s)	665, 1306, 1786, 1877, 2021
BtgI	CCRYGG	2 cut(s)	466, 1274
BtsI	GCAGTG	1 cut(s)	2411
Cac8I	GCNNGC	1 cut(s)	1388
CaiI	CAGNNNCTG	1 cut(s)	1997
CciI	TCATGA	1 cut(s)	2031
CfoI	GCGC	1 cut(s)	2028
Cfr13I	GGNCC	6 cut(s)	185, 576, 1262, 1304, 1876, 2020
Csp6I	GTAC	7 cut(s)	176, 955, 1327, 1630, 1858, 1970, 2473
CviAII	CATG	9 cut(s)	467, 485, 629, 935, 1275, 1280, 1446, 1586, 2032
CviQI	GTAC	7 cut(s)	176, 955, 1327, 1630, 1858, 1970, 2473
DraI	TTTAAA	1 cut(s)	910
DrdI	GACNNNNNNGTC	1 cut(s)	549
DseDI	GACNNNNNNGTC	1 cut(s)	549
EaeI	YGGCCR	2 cut(s)	663, 1784
Eam1104I	CTCTTC	2 cut(s)	373, 1249
EarI	CTCTTC	2 cut(s)	373, 1249
Ecl136II	GAGCTC	3 cut(s)	817, 2012, 2402
Eco130I	CCWWGG	6 cut(s)	466, 572, 1274, 1636, 1933, 2442
Eco24I	GRGCYC	3 cut(s)	819, 2014, 2404
Eco31I	GGTCTC	2 cut(s)	540, 1940
Eco32I	GATATC	5 cut(s)	403, 1285, 1669, 1867, 2375
Eco47I	GGWCC	3 cut(s)	185, 576, 1262
Eco53kI	GAGCTC	3 cut(s)	817, 2012, 2402
Eco57I	CTGAAG	2 cut(s)	1742, 1865
Eco88I	CYCGRG	4 cut(s)	237, 991, 1799, 2448
Eco91I	GGTNACC	3 cut(s)	607, 2348, 2360
EcoICRI	GAGCTC	3 cut(s)	817, 2012, 2402
EcoO109I	RGGNCCY	2 cut(s)	1304, 1876
EcoO65I	GGTNACC	3 cut(s)	607, 2348, 2360
EcoRI	GAATTC	1 cut(s)	480
EcoRII	CCWGG	4 cut(s)	649, 1237, 1569, 2344
EcoRV	GATATC	5 cut(s)	403, 1285, 1669, 1867, 2375
EcoT14I	CCWWGG	6 cut(s)	466, 572, 1274, 1636, 1933, 2442
EcoT22I	ATGCAT	2 cut(s)	1579, 2285
EcoT38I	GRGCYC	3 cut(s)	819, 2014, 2404
ErhI	CCWWGG	6 cut(s)	466, 572, 1274, 1636, 1933, 2442
FaeI	CATG	9 cut(s)	470, 488, 632, 938, 1278, 1283, 1449, 1589, 2035
FalI	AAGNNNNNCTT	2 cut(s)	2323, 2355
FaqI	GGGAC	4 cut(s)	18, 555, 1542, 2141
FatI	CATG	9 cut(s)	466, 484, 628, 934, 1274, 1279, 1445, 1585, 2031
FauNDI	CATATG	2 cut(s)	281, 1579
FbaI	TGATCA	1 cut(s)	937
FblI	GTMKAC	2 cut(s)	1764, 1795
Fnu4HI	GCNGC	1 cut(s)	1485
FriOI	GRGCYC	3 cut(s)	819, 2014, 2404
Fsp4HI	GCNGC	1 cut(s)	1485
FspBI	CTAG	8 cut(s)	227, 443, 477, 863, 1196, 1908, 2190, 2497
GlaI	GCGC	1 cut(s)	2027
GluI	GCNGC	1 cut(s)	1485
GsaI	CCCAGC	1 cut(s)	2189
GsuI	CTGGAG	3 cut(s)	1959, 1968, 2418
HaeII	RGCGCY	1 cut(s)	2029
HaeIII	GGCC	5 cut(s)	665, 1306, 1786, 1877, 2021
HapII	CCGG	5 cut(s)	1942, 2023, 2287, 2358, 2417
HhaI	GCGC	1 cut(s)	2028
Hin1II	CATG	9 cut(s)	470, 488, 632, 938, 1278, 1283, 1449, 1589, 2035
Hin6I	GCGC	1 cut(s)	2026
HinP1I	GCGC	1 cut(s)	2026
HincII	GTYRAC	1 cut(s)	1078
HindII	GTYRAC	1 cut(s)	1078
HindIII	AAGCTT	1 cut(s)	1388
HpaII	CCGG	5 cut(s)	1942, 2023, 2287, 2358, 2417
HphI	GGTGA	9 cut(s)	1046, 1199, 1373, 1400, 2227, 2291, 2305, 2354, 2360
Hpy166II	GTNNAC	9 cut(s)	55, 424, 1050, 1078, 1408, 1765, 1796, 1860, 2044
Hpy8I	GTNNAC	9 cut(s)	55, 424, 1050, 1078, 1408, 1765, 1796, 1860, 2044
HpyAV	CCTTC	9 cut(s)	280, 1295, 1784, 1889, 1948, 2011, 2363, 2375, 2486
HpyF10VI	GCNNNNNNNGC	5 cut(s)	242, 518, 987, 2018, 2147
Hsp92II	CATG	9 cut(s)	470, 488, 632, 938, 1278, 1283, 1449, 1589, 2035
HspAI	GCGC	1 cut(s)	2026
Kpn2I	TCCGGA	1 cut(s)	2286
Ksp22I	TGATCA	1 cut(s)	937
LguI	GCTCTTC	2 cut(s)	373, 1249
LmnI	GCTCC	4 cut(s)	1481, 1711, 2147, 2399
Lsp1109I	GCAGC	1 cut(s)	1471
LweI	GCATC	7 cut(s)	118, 508, 703, 1564, 1575, 1705, 2292
MaeI	CTAG	8 cut(s)	227, 443, 477, 863, 1196, 1908, 2190, 2497
MaeIII	GTNAC	8 cut(s)	88, 607, 1361, 1716, 2215, 2333, 2348, 2360
MflI	RGATCY	5 cut(s)	192, 787, 1099, 2087, 2320
MhlI	GDGCHC	6 cut(s)	313, 819, 2014, 2046, 2235, 2404
MlsI	TGGCCA	2 cut(s)	665, 1786
MluNI	TGGCCA	2 cut(s)	665, 1786
MlyI	GAGTC	5 cut(s)	50, 537, 1975, 2123, 2460
MmeI	TCCRAC	1 cut(s)	1114
Mox20I	TGGCCA	2 cut(s)	665, 1786
Mph1103I	ATGCAT	2 cut(s)	1579, 2285
MroI	TCCGGA	1 cut(s)	2286
MscI	TGGCCA	2 cut(s)	665, 1786
MseI	TTAA	5 cut(s)	108, 645, 909, 1686, 2238
MslI	CAYNNNNRTG	2 cut(s)	691, 1651
Msp20I	TGGCCA	2 cut(s)	665, 1786
MspA1I	CMGCKG	1 cut(s)	1459
MspI	CCGG	5 cut(s)	1942, 2023, 2287, 2358, 2417
MspR9I	CCNGG	8 cut(s)	651, 1239, 1571, 1943, 2023, 2346, 2358, 2418
Mva1269I	GAATGC	2 cut(s)	1903, 2285
MvaI	CCWGG	4 cut(s)	651, 1239, 1571, 2346
MvnI	CGCG	1 cut(s)	635
MwoI	GCNNNNNNNGC	5 cut(s)	242, 518, 987, 2018, 2147
NciI	CCSGG	4 cut(s)	1943, 2023, 2358, 2418
NcoI	CCATGG	2 cut(s)	466, 1274
NdeI	CATATG	2 cut(s)	281, 1579
NlaIII	CATG	9 cut(s)	470, 488, 632, 938, 1278, 1283, 1449, 1589, 2035
NlaIV	GGNNCC	6 cut(s)	202, 364, 789, 998, 1940, 2461
NmeAIII	GCCGAG	2 cut(s)	38, 1622
NmuCI	GTSAC	6 cut(s)	1361, 1716, 2215, 2333, 2348, 2360
NsiI	ATGCAT	2 cut(s)	1579, 2285
NspI	RCATGY	1 cut(s)	632
NspV	TTCGAA	2 cut(s)	961, 2424
PaeR7I	CTCGAG	1 cut(s)	2448
PagI	TCATGA	1 cut(s)	2031
PciSI	GCTCTTC	2 cut(s)	373, 1249
PcsI	WCGNNNNNNNCGW	1 cut(s)	244
PctI	GAATGC	2 cut(s)	1903, 2285
PfeI	GAWTC	9 cut(s)	623, 655, 675, 1120, 1504, 1624, 2263, 2270, 2296
PfoI	TCCNGGA	1 cut(s)	1569
PkrI	GCNGC	1 cut(s)	1486
PleI	GAGTC	5 cut(s)	50, 537, 1975, 2123, 2459
PpsI	GAGTC	5 cut(s)	50, 537, 1975, 2123, 2459
PshBI	ATTAAT	1 cut(s)	1686
Psp124BI	GAGCTC	3 cut(s)	819, 2014, 2404
Psp6I	CCWGG	4 cut(s)	649, 1237, 1569, 2344
PspEI	GGTNACC	3 cut(s)	607, 2348, 2360
PspFI	CCCAGC	1 cut(s)	2185
PspGI	CCWGG	4 cut(s)	649, 1237, 1569, 2344
PspN4I	GGNNCC	6 cut(s)	202, 364, 789, 998, 1940, 2461
PspPI	GGNCC	6 cut(s)	185, 576, 1262, 1304, 1876, 2020
PspXI	VCTCGAGB	1 cut(s)	2448
PstNI	CAGNNNCTG	1 cut(s)	1997
PsuI	RGATCY	5 cut(s)	192, 787, 1099, 2087, 2320
PvuII	CAGCTG	1 cut(s)	1459
RsaI	GTAC	7 cut(s)	177, 956, 1328, 1631, 1859, 1971, 2474
RsaNI	GTAC	7 cut(s)	176, 955, 1327, 1630, 1858, 1970, 2473
RseI	CAYNNNNRTG	2 cut(s)	691, 1651
SacI	GAGCTC	3 cut(s)	819, 2014, 2404
SapI	GCTCTTC	2 cut(s)	373, 1249
SaqAI	TTAA	5 cut(s)	108, 645, 909, 1686, 2238
SatI	GCNGC	1 cut(s)	1485
Sau96I	GGNCC	6 cut(s)	185, 576, 1262, 1304, 1876, 2020
ScaI	AGTACT	2 cut(s)	956, 1631
SchI	GAGTC	5 cut(s)	50, 537, 1975, 2123, 2460
ScrFI	CCNGG	8 cut(s)	651, 1239, 1571, 1943, 2023, 2346, 2358, 2418
SduI	GDGCHC	6 cut(s)	313, 819, 2014, 2046, 2235, 2404
SfaNI	GCATC	7 cut(s)	118, 508, 703, 1564, 1575, 1705, 2292
SfcI	CTRYAG	1 cut(s)	304
Sfr274I	CTCGAG	1 cut(s)	2448
SfuI	TTCGAA	2 cut(s)	961, 2424
SinI	GGWCC	3 cut(s)	185, 576, 1262
SlaI	CTCGAG	1 cut(s)	2448
SmiMI	CAYNNNNRTG	2 cut(s)	691, 1651
SmlI	CTYRAG	5 cut(s)	502, 836, 1391, 2329, 2448
SmoI	CTYRAG	5 cut(s)	502, 836, 1391, 2329, 2448
SsiI	CCGC	4 cut(s)	183, 635, 1260, 2306
SspMI	CTAG	8 cut(s)	227, 443, 477, 863, 1196, 1908, 2190, 2497
SstI	GAGCTC	3 cut(s)	819, 2014, 2404
StyD4I	CCNGG	8 cut(s)	649, 1237, 1569, 1941, 2021, 2344, 2356, 2416
StyI	CCWWGG	6 cut(s)	466, 572, 1274, 1636, 1933, 2442
TaqI	TCGA	6 cut(s)	961, 1247, 1473, 2371, 2424, 2449
TatI	WGTACW	4 cut(s)	954, 1326, 1629, 1857
TfiI	GAWTC	9 cut(s)	623, 655, 675, 1120, 1504, 1624, 2263, 2270, 2296
Tru1I	TTAA	5 cut(s)	108, 645, 909, 1686, 2238
Tru9I	TTAA	5 cut(s)	108, 645, 909, 1686, 2238
TseFI	GTSAC	6 cut(s)	1361, 1716, 2215, 2333, 2348, 2360
TseI	GCWGC	1 cut(s)	1484
Tsp45I	GTSAC	6 cut(s)	1361, 1716, 2215, 2333, 2348, 2360
TspDTI	ATGAA	7 cut(s)	307, 462, 473, 965, 1600, 2020, 2085
VneI	GTGCAC	1 cut(s)	2042
VpaK11BI	GGWCC	3 cut(s)	185, 576, 1262
VspI	ATTAAT	1 cut(s)	1686
XapI	RAATTY	2 cut(s)	480, 1606
XbaI	TCTAGA	2 cut(s)	226, 476
XceI	RCATGY	1 cut(s)	632
XcmI	CCANNNNNNNNNTGG	2 cut(s)	693, 1272
XhoI	CTCGAG	1 cut(s)	2448
XmiI	GTMKAC	2 cut(s)	1764, 1795
XspI	CTAG	8 cut(s)	227, 443, 477, 863, 1196, 1908, 2190, 2497
ZrmI	AGTACT	2 cut(s)	956, 1631
Zsp2I	ATGCAT	2 cut(s)	1579, 2285

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.