MD15G1052800.v1.1

Pentatricopeptide repeat-containing protein At1g63400-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008873
Physical Location & Seq
Forward (+)
3620154 .. 3621903
1750 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1052800.v1.1.491

Sequence Viewer

Length: 1707 bp
ATGGCTACACCAGGCAGAGCCCTAATCAACCCATCTCTCACATCTACAGATTTCAATGTGGCAGTCCGCATAAACTCCCTTCTGAAAATTCTAAACCCACAAAAACCCGACCGCTCTAATCTGGGTTTAGCGGCTCTCAACCGTTTCTCGCCTCTCCTGAAGCCAAGCCTTGTAATCCACGTAATCAAATCTCAGGCCAACCCTCACCGTGCCCTCTTCTTCTTCAACTGGGCTTCAAACCCTAACCCTAATCCCAACAACTATTCCCACACTCACCATTGCTACGTGGCCATCACCGACCTCCTCCTCTCCCATTCTCTCTTCTCCACCGCTGCCTCACTCCTTCAAGAATCCAATAGACTCAATGATTTCTTGGTCAGCAGGTTTATCGCAGTTCATGGCGGACGAGGGGATATCGGAGCCGCCATGGATTGGTTTCACAAGGCGAAGTTGATCGAGAATGGGAAATGCTTGTTTTCGTACAATGCGATTTTGGGTGCTCTGGTTAGGGCTGACAGGATGAGTGTAGCTAAGGAAATATATGATCAGATTGTGAAGGAAGGTATGGTGAAGCCCGATGTCTCGACTTGTAGCGTAATGATTAATGGGTTTTGCAAAATGGGTGAAATGGAGAATGCCCAGAAGGTGTTTGATGAAATGATTTGTGAGCCAGATGTGATTACTTACAGTACCATGATTCGTGGGTTTTGTAAGATGGGTGATTTTGTTAGCGCAAGGAAGGTTTGTGGTCAGATGAGGGAGAAAAAGGATTGTTTGCCCAATACAGTAACATATTCAATTTTGATTGACGGGTATTGCAAGAAAGGCGAGTTGGATGAGGCGATCAAGTGTATGAATGAAATGGAGGAGCAGGGATGTGAGCCCAATCAAGTTACTTACAGTGCTCTGATTCATGGATTTTGTAAAAAGGGTGATCTTGATAGTGCAAGGAAAGTGTTTGATAAAATGATTTGTGAGCCAGATGTGATTACTTACAATACCATGATTCTTGGATTTTGTAAAAAGGGTGATCTTGATAGTGCAAGGAAGGTGTTTGATAAAATGATTTGTGAGCCAGATGTGATTACTTACAATAACATGATTCTTGGATTTTGTAAAAAGGGTGATCTTGATAGTGCAAGGAAAGTGTTTTGTCAGATGAGGGAGAAAAAGGATTGTTTGCCCAATACAGTAACTTATTCAATTTTGATTGATGGGTATTGTAAGAAAGGCGAGTTGGAGGAGGCGATCAAGTGTATGAATGAAATGGAGAAGCAGGGTTGTGAGCCTAATATGTTTACTTGCAGTTCTTTAATTCATGGTTTCTGTAAGACAGGTGATTTGGATAGGGCGAGGCGAGTTTTGAGTCGGATGAGGGAGAGCAAGGATTGTTTGCCCAATACGGTAACTTATACAACTTTGATTGACGGGTATTGTAAGAAAGGCGAGTTGGAGGAGGCGATGAAGTGCATGAGTGAAATGGAGAAGCAGGGATGTGAGCCCAATCAAGTTACTTACAGTGCTCTGATTCATGGATTTTGTAAAATGGGTGAAATGGAGAATGCTCAGAAACTGTTTGATGAAATGACCGGCGACCCTGATCTGATTACTTACAATACTATGATTCATGGGTTTTGTAAGACGGGTGATTTTGATAGCGCGAGGCGAGTTTTGAGTCAGATGAGGAAAGAAGGGCGAGTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR_1 PPR_2 PPR_3

Protein Analysis

569

Amino Acids

63.85

Weight (kDa)

7.38

Isoelectric Point (pI)

26.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 157 - 206 4e-10 PPR repeat family
PPR_1 PF12854 190 - 220 1.7e-09 PPR repeat
PPR_2 PF13041 192 - 238 2.8e-15 PPR repeat family
PPR PF01535 196 - 221 7.1e-07 PPR repeat
PPR_1 PF12854 222 - 253 3e-10 PPR repeat
PPR_3 PF13812 223 - 272 1.1e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 224 - 274 1.8e-15 PPR repeat family
PPR PF01535 227 - 255 3e-06 PPR repeat
PPR_1 PF12854 256 - 289 1.2e-14 PPR repeat
PPR_3 PF13812 257 - 306 4.2e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 260 - 309 3.3e-22 PPR repeat family
TPR_24 PF23276 262 - 354 4.4e-07 Fungal tetratrico peptide repeats
PPR PF01535 263 - 293 2.3e-10 PPR repeat
PPR_long PF17177 279 - 356 2e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 285 - 336 1.2e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 292 - 323 2e-15 PPR repeat
PPR_2 PF13041 298 - 324 3e-06 PPR repeat family
PPR PF01535 298 - 324 3.8e-08 PPR repeat
PPR_1 PF12854 325 - 355 2.5e-14 PPR repeat
PPR_3 PF13812 326 - 368 5.8e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 327 - 356 5.1e-09 PPR repeat family
PPR PF01535 330 - 356 4e-08 PPR repeat
PPR_1 PF12854 357 - 388 6.6e-12 PPR repeat
PPR_3 PF13812 358 - 407 2.9e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 359 - 409 5.3e-19 PPR repeat family
PPR PF01535 362 - 390 7.5e-08 PPR repeat
PPR_1 PF12854 391 - 424 4.9e-14 PPR repeat
PPR_2 PF13041 395 - 444 4e-21 PPR repeat family
PPR PF01535 398 - 428 2.5e-10 PPR repeat
PPR_long PF17177 417 - 494 2.9e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 420 - 471 7e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 427 - 459 3.9e-10 PPR repeat
PPR_2 PF13041 433 - 480 3.7e-13 PPR repeat family
PPR PF01535 433 - 461 5.1e-06 PPR repeat
PPR_3 PF13812 462 - 513 1.5e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 462 - 495 3.4e-14 PPR repeat
PPR_2 PF13041 466 - 515 3.1e-23 PPR repeat family
PPR PF01535 469 - 499 9e-11 PPR repeat
PPR_long PF17177 485 - 563 1.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 498 - 530 4e-15 PPR repeat
PPR PF01535 504 - 530 4.8e-08 PPR repeat
PPR_2 PF13041 504 - 529 2.2e-06 PPR repeat family
PPR_1 PF12854 533 - 562 1.5e-11 PPR repeat
PPR_2 PF13041 533 - 565 3.3e-09 PPR repeat family
PPR PF01535 536 - 565 1e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 372
AccB7I CCANNNNNTGG 1 cut(s) 432
AccBSI CCGCTC 1 cut(s) 114
AccII CGCG 1 cut(s) 1661
AciI CCGC 6 cut(s) 67, 112, 131, 330, 402, 423
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 1 cut(s) 87
AcuI CTGAAG 1 cut(s) 179
AfaI GTAC 2 cut(s) 482, 691
AfiI CCNNNNNNNGG 1 cut(s) 432
AgsI TTSAA 6 cut(s) 55, 226, 237, 347, 798, 1203
AjnI CCWGG 1 cut(s) 10
AjuI GAANNNNNNNTTGG 7 cut(s) 815, 847, 1220, 1252, 1433, 1465, 1683
AluBI AGCT 1 cut(s) 530
AluI AGCT 1 cut(s) 530
Alw21I GWGCWC 3 cut(s) 502, 907, 1525
Alw26I GTCTC 1 cut(s) 586
AlwNI CAGNNNCTG 1 cut(s) 1573
AoxI GGCC 2 cut(s) 195, 288
ApeKI GCWGC 1 cut(s) 332
ApoI RAATTY 1 cut(s) 87
ArsI GACNNNNNNTTYG 2 cut(s) 1324, 1356
AseI ATTAAT 1 cut(s) 603
AspLEI GCGC 2 cut(s) 734, 1661
BaeGI GKGCMC 1 cut(s) 214
BalI TGGCCA 1 cut(s) 290
BanII GRGCYC 3 cut(s) 22, 885, 1503
Bbv12I GWGCWC 3 cut(s) 502, 907, 1525
BbvI GCAGC 1 cut(s) 319
BccI CCATC 4 cut(s) 40, 299, 709, 1208
BcgI CGANNNNNNTGC 2 cut(s) 370, 404
BciT130I CCWGG 1 cut(s) 12
BclI TGATCA 1 cut(s) 544
BcoDI GTCTC 1 cut(s) 586
BfmI CTRYAG 1 cut(s) 45
BfuAI ACCTGC 1 cut(s) 372
BisI GCNGC 3 cut(s) 132, 333, 423
BlsI GCNGC 3 cut(s) 133, 334, 424
Bme1390I CCNGG 1 cut(s) 12
BmiI GGNNCC 1 cut(s) 421
BmrFI CCNGG 1 cut(s) 12
BmrI ACTGGG 1 cut(s) 238
BmuI ACTGGG 1 cut(s) 238
Bpu10I CCTNAGC 1 cut(s) 531
BsaAI YACGTR 2 cut(s) 181, 286
BsaJI CCNNGG 1 cut(s) 426
Bsc4I CCNNNNNNNGG 1 cut(s) 432
Bse118I RCCGGY 1 cut(s) 1589
Bse1I ACTGG 1 cut(s) 233
Bse3DI GCAATG 1 cut(s) 277
BseBI CCWGG 1 cut(s) 12
BseDI CCNNGG 1 cut(s) 426
BseGI GGATG 5 cut(s) 525, 841, 881, 1377, 1499
BseLI CCNNNNNNNGG 1 cut(s) 432
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 2 cut(s) 206, 1580
BseNI ACTGG 1 cut(s) 233
BseRI GAGGAG 5 cut(s) 293, 296, 881, 1256, 1469
BseSI GKGCMC 1 cut(s) 214
BseXI GCAGC 1 cut(s) 319
Bsh1236I CGCG 1 cut(s) 1661
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 2 cut(s) 197, 290
BsiEI CGRYCG 1 cut(s) 112
BsiHKAI GWGCWC 3 cut(s) 502, 907, 1525
BsiSI CCGG 1 cut(s) 1590
BslI CCNNNNNNNGG 1 cut(s) 432
BsmAI GTCTC 1 cut(s) 586
BsmI GAATGC 2 cut(s) 640, 1567
BsnI GGCC 2 cut(s) 197, 290
Bsp1286I GDGCHC 7 cut(s) 22, 214, 502, 885, 907, 1503, 1525
Bsp143I GATC 8 cut(s) 453, 544, 843, 934, 1030, 1126, 1248, 1600
Bsp19I CCATGG 1 cut(s) 426
BspACI CCGC 6 cut(s) 67, 112, 131, 330, 402, 423
BspANI GGCC 2 cut(s) 197, 290
BspCNI CTCAG 2 cut(s) 205, 1579
BspFNI CGCG 1 cut(s) 1661
BspLI GGNNCC 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 372
BsrBI CCGCTC 1 cut(s) 114
BsrDI GCAATG 1 cut(s) 277
BsrFI RCCGGY 1 cut(s) 1589
BsrI ACTGG 1 cut(s) 233
BssAI RCCGGY 1 cut(s) 1589
BssECI CCNNGG 1 cut(s) 426
BssMI GATC 8 cut(s) 453, 544, 843, 934, 1030, 1126, 1248, 1600
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 12
Bst4CI ACNGT 9 cut(s) 143, 209, 689, 787, 902, 1192, 1405, 1520, 1575
Bst6I CTCTTC 2 cut(s) 221, 326
BstBAI YACGTR 2 cut(s) 181, 286
BstDEI CTNAG 3 cut(s) 192, 531, 1566
BstDSI CCRYGG 1 cut(s) 426
BstF5I GGATG 5 cut(s) 525, 841, 881, 1377, 1499
BstFNI CGCG 1 cut(s) 1661
BstHHI GCGC 2 cut(s) 734, 1661
BstKTI GATC 8 cut(s) 456, 547, 846, 937, 1033, 1129, 1251, 1603
BstMAI GTCTC 1 cut(s) 586
BstMBI GATC 8 cut(s) 453, 544, 843, 934, 1030, 1126, 1248, 1600
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 1 cut(s) 825
BstNI CCWGG 1 cut(s) 12
BstSCI CCNGG 1 cut(s) 10
BstSFI CTRYAG 1 cut(s) 45
BstSLI GKGCMC 1 cut(s) 214
BstUI CGCG 1 cut(s) 1661
BstV1I GCAGC 1 cut(s) 319
BsuRI GGCC 2 cut(s) 197, 290
BtgI CCRYGG 1 cut(s) 426
BtgZI GCGATG 1 cut(s) 1475
BtsCI GGATG 5 cut(s) 525, 841, 881, 1377, 1499
BtsIMutI CAGTG 2 cut(s) 907, 1525
BveI ACCTGC 1 cut(s) 372
CaiI CAGNNNCTG 1 cut(s) 1573
CfoI GCGC 2 cut(s) 734, 1661
Cfr10I RCCGGY 1 cut(s) 1589
Csp6I GTAC 2 cut(s) 481, 690
CviQI GTAC 2 cut(s) 481, 690
DdeI CTNAG 3 cut(s) 192, 531, 1566
DpnI GATC 8 cut(s) 455, 546, 845, 936, 1032, 1128, 1250, 1602
DpnII GATC 8 cut(s) 453, 544, 843, 934, 1030, 1126, 1248, 1600
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 2 cut(s) 221, 326
EarI CTCTTC 2 cut(s) 221, 326
EciI GGCGGA 1 cut(s) 417
Eco130I CCWWGG 1 cut(s) 426
Eco24I GRGCYC 3 cut(s) 22, 885, 1503
Eco32I GATATC 1 cut(s) 415
Eco57I CTGAAG 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 10
EcoRV GATATC 1 cut(s) 415
EcoT14I CCWWGG 1 cut(s) 426
EcoT38I GRGCYC 3 cut(s) 22, 885, 1503
ErhI CCWWGG 1 cut(s) 426
FbaI TGATCA 1 cut(s) 544
Fnu4HI GCNGC 3 cut(s) 132, 333, 423
FokI GGATG 5 cut(s) 532, 848, 888, 1384, 1506
FriOI GRGCYC 3 cut(s) 22, 885, 1503
Fsp4HI GCNGC 3 cut(s) 132, 333, 423
GlaI GCGC 2 cut(s) 733, 1660
GluI GCNGC 3 cut(s) 132, 333, 423
HaeIII GGCC 2 cut(s) 197, 290
HapII CCGG 1 cut(s) 1590
HhaI GCGC 2 cut(s) 734, 1661
Hin6I GCGC 2 cut(s) 732, 1659
HinP1I GCGC 2 cut(s) 732, 1659
HpaII CCGG 1 cut(s) 1590
Hpy166II GTNNAC 1 cut(s) 1299
Hpy188III TCNNGA 7 cut(s) 157, 347, 457, 583, 938, 1034, 1130
Hpy8I GTNNAC 1 cut(s) 1299
HpyAV CCTTC 8 cut(s) 89, 353, 550, 554, 637, 733, 1042, 1685
HpyCH4III ACNGT 9 cut(s) 143, 209, 689, 787, 902, 1192, 1405, 1520, 1575
HpyCH4IV ACGT 2 cut(s) 180, 285
HpyCH4V TGCA 7 cut(s) 615, 819, 947, 1043, 1139, 1305, 1470
HpyF10VI GCNNNNNNNGC 1 cut(s) 825
HpyF3I CTNAG 3 cut(s) 192, 531, 1566
HpySE526I ACGT 2 cut(s) 180, 285
HspAI GCGC 2 cut(s) 732, 1659
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 8 cut(s) 453, 544, 843, 934, 1030, 1126, 1248, 1600
LmnI GCTCC 2 cut(s) 419, 868
Lsp1109I GCAGC 1 cut(s) 319
MaeII ACGT 2 cut(s) 180, 285
MaeIII GTNAC 5 cut(s) 787, 892, 1192, 1405, 1510
MalI GATC 8 cut(s) 455, 546, 845, 936, 1032, 1128, 1250, 1602
MbiI CCGCTC 1 cut(s) 114
MboI GATC 8 cut(s) 453, 544, 843, 934, 1030, 1126, 1248, 1600
MboII GAAGA 4 cut(s) 208, 211, 214, 313
MhlI GDGCHC 7 cut(s) 22, 214, 502, 885, 907, 1503, 1525
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 4 cut(s) 87, 798, 1203, 1314
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 3 cut(s) 354, 1375, 1684
MmeI TCCRAC 5 cut(s) 813, 1218, 1349, 1431, 1681
Mox20I TGGCCA 1 cut(s) 290
MscI TGGCCA 1 cut(s) 290
MseI TTAA 2 cut(s) 603, 1313
Msp20I TGGCCA 1 cut(s) 290
MspA1I CMGCKG 1 cut(s) 332
MspI CCGG 1 cut(s) 1590
MspR9I CCNGG 1 cut(s) 12
Mva1269I GAATGC 2 cut(s) 640, 1567
MvaI CCWGG 1 cut(s) 12
MvnI CGCG 1 cut(s) 1661
MwoI GCNNNNNNNGC 1 cut(s) 825
NcoI CCATGG 1 cut(s) 426
NdeII GATC 8 cut(s) 453, 544, 843, 934, 1030, 1126, 1248, 1600
NlaIV GGNNCC 1 cut(s) 421
PcsI WCGNNNNNNNCGW 1 cut(s) 485
PctI GAATGC 2 cut(s) 640, 1567
PfeI GAWTC 7 cut(s) 350, 697, 910, 1006, 1102, 1528, 1624
PflMI CCANNNNNTGG 1 cut(s) 432
PkrI GCNGC 3 cut(s) 133, 334, 424
PleI GAGTC 3 cut(s) 354, 1374, 1683
PpsI GAGTC 3 cut(s) 354, 1374, 1683
Ppu21I YACGTR 2 cut(s) 181, 286
PshBI ATTAAT 1 cut(s) 603
Psp6I CCWGG 1 cut(s) 10
PspGI CCWGG 1 cut(s) 10
PspN4I GGNNCC 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 1573
RsaI GTAC 2 cut(s) 482, 691
RsaNI GTAC 2 cut(s) 481, 690
SaqAI TTAA 2 cut(s) 603, 1313
SatI GCNGC 3 cut(s) 132, 333, 423
Sau3AI GATC 8 cut(s) 453, 544, 843, 934, 1030, 1126, 1248, 1600
SchI GAGTC 3 cut(s) 354, 1375, 1684
ScrFI CCNGG 1 cut(s) 12
SduI GDGCHC 7 cut(s) 22, 214, 502, 885, 907, 1503, 1525
SfcI CTRYAG 1 cut(s) 45
Sse9I AATT 4 cut(s) 87, 798, 1203, 1314
SsiI CCGC 6 cut(s) 67, 112, 131, 330, 402, 423
StyD4I CCNGG 1 cut(s) 10
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 9 cut(s) 143, 209, 689, 787, 902, 1192, 1405, 1520, 1575
TaiI ACGT 2 cut(s) 183, 288
TaqI TCGA 2 cut(s) 456, 584
TasI AATT 4 cut(s) 87, 798, 1203, 1314
TauI GCSGC 2 cut(s) 134, 425
TfiI GAWTC 7 cut(s) 350, 697, 910, 1006, 1102, 1528, 1624
Tru1I TTAA 2 cut(s) 603, 1313
Tru9I TTAA 2 cut(s) 603, 1313
TscAI CASTG 2 cut(s) 907, 1525
TseI GCWGC 1 cut(s) 332
TspRI CASTG 2 cut(s) 907, 1525
Van91I CCANNNNNTGG 1 cut(s) 432
VspI ATTAAT 1 cut(s) 603
XapI RAATTY 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.