RchiOBHm_Chr6g0311521

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008873
Physical Location & Seq
Reverse (-)
68390827 .. 68393226
2400 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28017

Sequence Viewer

Length: 1662 bp
ATGGCTGCACCAGCTAGAGCCCTAAACCCATTTCACACCTCTACCAATCACTCTCAAGTGACATTCATAACCTCCCTCCTGCAAACCCTAAACCCACAGACACCTAACCCCTCAAACCTGAGCTCAGCCCCTCTTAACCAGTTCTCACCTCACCTAAACCCAACCCTAGTTATCCAAGTCCTCCACAACCAGACCAATCCCTACCACGCCCTCTTCTTCTTCAAATGGGCATCAAACCCTGACCACAATCCCAACAACTACTCCCACACCTTCAAATGCTACTCGGCCATCACTGACATCCTCCTCTCCCACTCCCTCTTCTCCTCCGCCTCATCACTCCTCCAAGAATCCAATCACCTCTCTGATTTTCTGATCGGCAAGTTCATCACAGCTCACGGCCGCCGCCGCGGTGTGAGGGCCGCCATTAATTGGTTTCACAAGGCGAAAGTGATTGAGAATGGGAGGTGCTTGTTTTCTTACAATGCAATTCTGGGTGTGTTGGTTAGGGCAAATAGGATTGGTTTAGCTGAGTCAATTTATGATCAGATTTTCAAGGAAGGTCTGGTGAAACCGGATGCGTCGACTTATACCATAATGATTAGAGGGTTTTGTAAAGTGGGTGAGATTGAGAATGCACAGAAGGTGTTCGATGAAATGACTTGTGAGCCGAATTTGATTACTTACAATACTATGATTCATGGGTTCTGCAAGAAGGGTGAGGTTGATAGCGCGAGGAGAGTTTTGGGTCAGATGATGGAGGGTAAGGATTGCTTGCCTGATACGGTAACTTATACAACTTTGATTGATGGGTATAGTAAGAAAGGTGAGTTGGGCGAGGCAATGAAGTGTATGGATGAGATGGTGAAACAGGCTTGTGAGCCTAATGTGTTGACTTACAATGCGGTGATTCATGGTTTTTGTTTGAGTGGGAATGTTAATGAGGCAAAGAGGATGATGACGAGGATGAGGTTGAATGGTGTGAAGGATGATATAGCGACGCATACGAGTATATTGAAAGGGCTTTGCATTGCGGGGAAGGCTGATGAAGCTGTTGAGCATCTTAAAGAGATGGCCAGACTTGGGATGAAACTGGATGTGAAAGCGTACGGAATTGTTGTCAATGAGTATTGTAAGAGGAAAGAACCGGATGGAGCAATTTTGCTTTTAATGGAAATGAGAATGAGAGGCCTGAAGCCAAGTGTTTCGAGCTTTAATGCAGTGCTGAGTGTTCTTCTTGAAAATGGGGAGCTCGAAAGAGCCATTAATCTTGTAAAGCAAATGCCACAAATGGATTGCTTTCCCAATTTCTTTTCATACAACTCAGTGATATGTAGTCTTTGTAATGTGAGAGGTAGAATGGGGGAAGTTGAAGGGCTTATCCATGACATGCTTCGAAATGGTCATGCCCTTGATGCCACGCTCTGTAGTTGCTTGGTCAAAGGTTATTCCGAGGATGGTGATTTGAGAAGCGCAATGCAGGTTTTCTGTGACGCACTTGATCAGAGGTACATTATTAGTTTGGAGAGCTTTTCAGTTTTCGTCAAAGAGTTATGCGCCAAGGGGAAGGCTGTTGAGGCTGAGAGGATGTTTGAGGATATGTGTAAAAGATGCAGTGTGGTTCATATAGATATTTACAGAAAGGTGTTAGATGATTATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

553

Amino Acids

61.93

Weight (kDa)

7.51

Isoelectric Point (pI)

25.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 157 - 205 6.5e-11 PPR repeat family
PPR_1 PF12854 189 - 220 6e-12 PPR repeat
PPR_3 PF13812 189 - 234 8.7e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 191 - 220 2e-07 PPR repeat family
PPR PF01535 194 - 220 2.5e-07 PPR repeat
PPR_1 PF12854 220 - 251 3.7e-14 PPR repeat
PPR_2 PF13041 223 - 271 2e-15 PPR repeat family
PPR PF01535 226 - 253 2.3e-07 PPR repeat
PPR_long PF17177 251 - 356 2e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 251 - 304 7.9e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 255 - 288 3e-11 PPR repeat
PPR_2 PF13041 259 - 307 4.9e-19 PPR repeat family
PPR PF01535 262 - 289 4.2e-08 PPR repeat
PPR_1 PF12854 292 - 323 3.4e-11 PPR repeat
PPR_2 PF13041 294 - 342 4.5e-12 PPR repeat family
TPR_24 PF23276 298 - 381 1.3e-06 Fungal tetratrico peptide repeats
PPR PF01535 298 - 327 1e-05 PPR repeat
PPR_long PF17177 313 - 423 4.3e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 325 - 357 5.8e-06 PPR repeat
PPR_2 PF13041 330 - 378 9.8e-08 PPR repeat family
PPR_3 PF13812 354 - 411 1.3e-06 Pentatricopeptide repeat domain
PPR_3 PF13812 388 - 443 4.2e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 399 - 448 1.9e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1468
AccB7I CCANNNNNTGG 1 cut(s) 429
AccI GTMKAC 1 cut(s) 581
AccII CGCG 2 cut(s) 408, 731
AciI CCGC 8 cut(s) 327, 400, 403, 406, 408, 420, 902, 1031
AcoI YGGCCR 3 cut(s) 285, 397, 1071
AcsI RAATTY 1 cut(s) 670
AcuI CTGAAG 1 cut(s) 1211
AfaI GTAC 2 cut(s) 1106, 1508
AfiI CCNNNNNNNGG 2 cut(s) 429, 1080
AgsI TTSAA 7 cut(s) 223, 274, 553, 973, 1015, 1238, 1370
AjuI GAANNNNNNNTTGG 2 cut(s) 812, 844
AluBI AGCT 8 cut(s) 14, 123, 392, 527, 1049, 1209, 1249, 1527
AluI AGCT 8 cut(s) 14, 123, 392, 527, 1049, 1209, 1249, 1527
Alw21I GWGCWC 2 cut(s) 125, 1251
AoxI GGCC 5 cut(s) 285, 397, 417, 1071, 1186
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 670
AseI ATTAAT 2 cut(s) 426, 1263
Asp700I GAANNNNTTC 1 cut(s) 644
AspLEI GCGC 3 cut(s) 731, 1472, 1556
AspS9I GGNCC 1 cut(s) 417
AsuII TTCGAA 1 cut(s) 1393
BalI TGGCCA 1 cut(s) 1073
BanII GRGCYC 3 cut(s) 22, 125, 1251
Bbv12I GWGCWC 2 cut(s) 125, 1251
BccI CCATC 7 cut(s) 296, 748, 800, 853, 1063, 1142, 1448
BceAI ACGGC 1 cut(s) 412
BclI TGATCA 2 cut(s) 541, 1498
BfaI CTAG 2 cut(s) 15, 167
BfmI CTRYAG 1 cut(s) 1423
BfuAI ACCTGC 1 cut(s) 1468
BisI GCNGC 5 cut(s) 6, 400, 403, 406, 420
BlpI GCTNAGC 1 cut(s) 124
BlsI GCNGC 5 cut(s) 7, 401, 404, 407, 421
BmgT120I GGNCC 1 cut(s) 417
BmsI GCATC 5 cut(s) 239, 565, 1066, 1402, 1598
Bpu10I CCTNAGC 1 cut(s) 119
Bpu1102I GCTNAGC 1 cut(s) 124
Bpu14I TTCGAA 1 cut(s) 1393
BpuEI CTTGAG 1 cut(s) 39
BsaJI CCNNGG 3 cut(s) 406, 1449, 1557
BsaWI WCCGGW 2 cut(s) 571, 1144
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 2 cut(s) 429, 1080
Bse1I ACTGG 2 cut(s) 139, 1095
Bse3DI GCAATG 3 cut(s) 846, 1026, 1479
BseDI CCNNGG 3 cut(s) 406, 1449, 1557
BseLI CCNNNNNNNGG 2 cut(s) 429, 1080
BseMI GCAATG 3 cut(s) 846, 1026, 1479
BseMII CTCAG 6 cut(s) 110, 138, 519, 1214, 1335, 1569
BseNI ACTGG 2 cut(s) 139, 1095
BseRI GAGGAG 4 cut(s) 293, 313, 329, 748
BseX3I CGGCCG 1 cut(s) 397
Bsh1236I CGCG 2 cut(s) 408, 731
Bsh1285I CGRYCG 1 cut(s) 400
BshFI GGCC 5 cut(s) 287, 399, 419, 1073, 1188
BsiEI CGRYCG 1 cut(s) 400
BsiHKAI GWGCWC 2 cut(s) 125, 1251
BsiSI CCGG 2 cut(s) 572, 1145
BsiWI CGTACG 1 cut(s) 1104
BslI CCNNNNNNNGG 2 cut(s) 429, 1080
BsmI GAATGC 1 cut(s) 637
BsnI GGCC 5 cut(s) 287, 399, 419, 1073, 1188
Bsp119I TTCGAA 1 cut(s) 1393
Bsp1286I GDGCHC 3 cut(s) 22, 125, 1251
Bsp143I GATC 3 cut(s) 372, 541, 1498
Bsp1720I GCTNAGC 1 cut(s) 124
BspACI CCGC 8 cut(s) 327, 400, 403, 406, 408, 420, 902, 1031
BspANI GGCC 5 cut(s) 287, 399, 419, 1073, 1188
BspCNI CTCAG 6 cut(s) 111, 137, 520, 1215, 1334, 1570
BspFNI CGCG 2 cut(s) 408, 731
BspMI ACCTGC 1 cut(s) 1468
BspT104I TTCGAA 1 cut(s) 1393
BsrDI GCAATG 3 cut(s) 846, 1026, 1479
BsrI ACTGG 2 cut(s) 139, 1095
BssECI CCNNGG 3 cut(s) 406, 1449, 1557
BssMI GATC 3 cut(s) 372, 541, 1498
BssT1I CCWWGG 1 cut(s) 1557
Bst4CI ACNGT 1 cut(s) 784
Bst6I CTCTTC 2 cut(s) 218, 323
BstBI TTCGAA 1 cut(s) 1393
BstC8I GCNNGC 1 cut(s) 773
BstDEI CTNAG 6 cut(s) 119, 124, 528, 1223, 1321, 1578
BstDSI CCRYGG 1 cut(s) 406
BstFNI CGCG 2 cut(s) 408, 731
BstHHI GCGC 3 cut(s) 731, 1472, 1556
BstKTI GATC 3 cut(s) 375, 544, 1501
BstMBI GATC 3 cut(s) 372, 541, 1498
BstMCI CGRYCG 1 cut(s) 400
BstMWI GCNNNNNNNGC 6 cut(s) 11, 405, 1037, 1046, 1412, 1574
BstNSI RCATGY 1 cut(s) 1390
BstSFI CTRYAG 1 cut(s) 1423
BstUI CGCG 2 cut(s) 408, 731
BstZI CGGCCG 1 cut(s) 397
BsuRI GGCC 5 cut(s) 287, 399, 419, 1073, 1188
BtgI CCRYGG 1 cut(s) 406
BtsI GCAGTG 2 cut(s) 1224, 1618
BtsIMutI CAGTG 4 cut(s) 291, 1224, 1329, 1618
BveI ACCTGC 1 cut(s) 1468
Cac8I GCNNGC 1 cut(s) 773
CfoI GCGC 3 cut(s) 731, 1472, 1556
Cfr13I GGNCC 1 cut(s) 417
Cfr42I CCGCGG 1 cut(s) 409
CseI GACGC 3 cut(s) 567, 1006, 1499
Csp6I GTAC 2 cut(s) 1105, 1507
CviAII CATG 5 cut(s) 698, 911, 1382, 1387, 1403
CviQI GTAC 2 cut(s) 1105, 1507
DdeI CTNAG 6 cut(s) 119, 124, 528, 1223, 1321, 1578
DpnI GATC 3 cut(s) 374, 543, 1500
DpnII GATC 3 cut(s) 372, 541, 1498
EaeI YGGCCR 3 cut(s) 285, 397, 1071
EagI CGGCCG 1 cut(s) 397
Eam1104I CTCTTC 2 cut(s) 218, 323
EarI CTCTTC 2 cut(s) 218, 323
EciI GGCGGA 1 cut(s) 316
Ecl136II GAGCTC 2 cut(s) 123, 1249
EclXI CGGCCG 1 cut(s) 397
Eco130I CCWWGG 1 cut(s) 1557
Eco147I AGGCCT 1 cut(s) 1188
Eco24I GRGCYC 3 cut(s) 22, 125, 1251
Eco52I CGGCCG 1 cut(s) 397
Eco53kI GAGCTC 2 cut(s) 123, 1249
Eco57I CTGAAG 1 cut(s) 1211
EcoICRI GAGCTC 2 cut(s) 123, 1249
EcoT14I CCWWGG 1 cut(s) 1557
EcoT38I GRGCYC 3 cut(s) 22, 125, 1251
ErhI CCWWGG 1 cut(s) 1557
FaeI CATG 5 cut(s) 701, 914, 1385, 1390, 1406
FalI AAGNNNNNCTT 2 cut(s) 755, 787
FatI CATG 5 cut(s) 697, 910, 1381, 1386, 1402
FauI CCCGC 1 cut(s) 1024
FbaI TGATCA 2 cut(s) 541, 1498
FblI GTMKAC 1 cut(s) 581
Fnu4HI GCNGC 5 cut(s) 6, 400, 403, 406, 420
FriOI GRGCYC 3 cut(s) 22, 125, 1251
Fsp4HI GCNGC 5 cut(s) 6, 400, 403, 406, 420
FspBI CTAG 2 cut(s) 15, 167
GlaI GCGC 3 cut(s) 730, 1471, 1555
GluI GCNGC 5 cut(s) 6, 400, 403, 406, 420
HaeIII GGCC 5 cut(s) 287, 399, 419, 1073, 1188
HapII CCGG 2 cut(s) 572, 1145
HgaI GACGC 3 cut(s) 567, 1006, 1499
HhaI GCGC 3 cut(s) 731, 1472, 1556
Hin1II CATG 5 cut(s) 701, 914, 1385, 1390, 1406
Hin6I GCGC 3 cut(s) 729, 1470, 1554
HinP1I GCGC 3 cut(s) 729, 1470, 1554
HincII GTYRAC 2 cut(s) 582, 891
HindII GTYRAC 2 cut(s) 582, 891
HinfI GANTC 4 cut(s) 347, 530, 694, 907
HpaII CCGG 2 cut(s) 572, 1145
Hpy166II GTNNAC 2 cut(s) 582, 891
Hpy188I TCNGA 6 cut(s) 364, 372, 546, 750, 1450, 1503
Hpy188III TCNNGA 1 cut(s) 1235
Hpy8I GTNNAC 2 cut(s) 582, 891
Hpy99I CGWCG 2 cut(s) 583, 1000
HpyAV CCTTC 8 cut(s) 280, 551, 634, 706, 976, 1030, 1364, 1558
HpyCH4III ACNGT 1 cut(s) 784
HpyCH4V TGCA 9 cut(s) 8, 82, 485, 635, 708, 1026, 1217, 1477, 1611
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 405, 1037, 1046, 1412, 1574
HpyF3I CTNAG 6 cut(s) 119, 124, 528, 1223, 1321, 1578
Hsp92II CATG 5 cut(s) 701, 914, 1385, 1390, 1406
HspAI GCGC 3 cut(s) 729, 1470, 1554
Ksp22I TGATCA 2 cut(s) 541, 1498
KspI CCGCGG 1 cut(s) 409
Kzo9I GATC 3 cut(s) 372, 541, 1498
LmnI GCTCC 2 cut(s) 1151, 1246
LweI GCATC 5 cut(s) 239, 565, 1066, 1402, 1598
MaeI CTAG 2 cut(s) 15, 167
MaeIII GTNAC 3 cut(s) 58, 784, 1487
MalI GATC 3 cut(s) 374, 543, 1500
MboI GATC 3 cut(s) 372, 541, 1498
MboII GAAGA 5 cut(s) 205, 208, 211, 310, 1223
MhlI GDGCHC 3 cut(s) 22, 125, 1251
MlsI TGGCCA 1 cut(s) 1073
MluCI AATT 7 cut(s) 427, 486, 534, 670, 1110, 1155, 1303
MluNI TGGCCA 1 cut(s) 1073
MlyI GAGTC 1 cut(s) 539
Mox20I TGGCCA 1 cut(s) 1073
MroXI GAANNNNTTC 1 cut(s) 644
MscI TGGCCA 1 cut(s) 1073
MseI TTAA 7 cut(s) 135, 426, 936, 1062, 1166, 1212, 1263
Msp20I TGGCCA 1 cut(s) 1073
MspA1I CMGCKG 1 cut(s) 408
MspI CCGG 2 cut(s) 572, 1145
Mva1269I GAATGC 1 cut(s) 637
MvnI CGCG 2 cut(s) 408, 731
MwoI GCNNNNNNNGC 6 cut(s) 11, 405, 1037, 1046, 1412, 1574
NdeII GATC 3 cut(s) 372, 541, 1498
NlaIII CATG 5 cut(s) 701, 914, 1385, 1390, 1406
NmeAIII GCCGAG 1 cut(s) 263
NmuCI GTSAC 2 cut(s) 58, 1487
NspI RCATGY 1 cut(s) 1390
NspV TTCGAA 1 cut(s) 1393
PceI AGGCCT 1 cut(s) 1188
PctI GAATGC 1 cut(s) 637
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 3 cut(s) 347, 694, 907
Pfl23II CGTACG 1 cut(s) 1104
PflMI CCANNNNNTGG 1 cut(s) 429
PkrI GCNGC 5 cut(s) 7, 401, 404, 407, 421
PleI GAGTC 1 cut(s) 538
PpsI GAGTC 1 cut(s) 538
PshBI ATTAAT 2 cut(s) 426, 1263
Psp124BI GAGCTC 2 cut(s) 125, 1251
PspLI CGTACG 1 cut(s) 1104
PspPI GGNCC 1 cut(s) 417
RsaI GTAC 2 cut(s) 1106, 1508
RsaNI GTAC 2 cut(s) 1105, 1507
SacI GAGCTC 2 cut(s) 125, 1251
SacII CCGCGG 1 cut(s) 409
SalI GTCGAC 1 cut(s) 580
SaqAI TTAA 7 cut(s) 135, 426, 936, 1062, 1166, 1212, 1263
SatI GCNGC 5 cut(s) 6, 400, 403, 406, 420
Sau3AI GATC 3 cut(s) 372, 541, 1498
Sau96I GGNCC 1 cut(s) 417
SchI GAGTC 1 cut(s) 539
SduI GDGCHC 3 cut(s) 22, 125, 1251
SfaNI GCATC 5 cut(s) 239, 565, 1066, 1402, 1598
SfcI CTRYAG 1 cut(s) 1423
Sfr303I CCGCGG 1 cut(s) 409
SfuI TTCGAA 1 cut(s) 1393
SgrBI CCGCGG 1 cut(s) 409
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
Sse9I AATT 7 cut(s) 427, 486, 534, 670, 1110, 1155, 1303
SseBI AGGCCT 1 cut(s) 1188
SsiI CCGC 8 cut(s) 327, 400, 403, 406, 408, 420, 902, 1031
SspMI CTAG 2 cut(s) 15, 167
SstI GAGCTC 2 cut(s) 125, 1251
StuI AGGCCT 1 cut(s) 1188
StyI CCWWGG 1 cut(s) 1557
TaaI ACNGT 1 cut(s) 784
TaqI TCGA 5 cut(s) 581, 648, 1205, 1251, 1393
TasI AATT 7 cut(s) 427, 486, 534, 670, 1110, 1155, 1303
TauI GCSGC 4 cut(s) 402, 405, 408, 422
TfiI GAWTC 3 cut(s) 347, 694, 907
Tru1I TTAA 7 cut(s) 135, 426, 936, 1062, 1166, 1212, 1263
Tru9I TTAA 7 cut(s) 135, 426, 936, 1062, 1166, 1212, 1263
TscAI CASTG 4 cut(s) 298, 1224, 1329, 1618
TseFI GTSAC 2 cut(s) 58, 1487
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 58, 1487
TspGWI ACGGA 1 cut(s) 1122
TspRI CASTG 4 cut(s) 298, 1224, 1329, 1618
Van91I CCANNNNNTGG 1 cut(s) 429
VspI ATTAAT 2 cut(s) 426, 1263
XapI RAATTY 1 cut(s) 670
XceI RCATGY 1 cut(s) 1390
XmiI GTMKAC 1 cut(s) 581
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 2 cut(s) 15, 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.