pycom08g05710

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008873
Physical Location & Seq
Reverse (-)
4332852 .. 4333787
936 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g05710.1

Sequence Viewer

Length: 936 bp
ATGAGGGAGAGTAAGGATTGCTTGCCTGGTACGGTAACTTATACAACTTTGATTGATGGGTATTGTAAGAAAGGCAAGTTGGAGGAGGCGATGAAGTGTATGAGTGAAATGGAGAAGCAGGGGTGTGAGCCTAATCTATTTACTTACAATGCTTTAATTCATGGTTTGTGTTTGAGTGGGAATGTTGGTGAGGCAATGAGGATGGTGATAAAGATGAGGTTAAATGGTGTGAAGGACGATATAGCAACACATACGGCTATCTTGAAAGGGCTTTGCATTGCAGGGAGGCCTGACGACGCTGCTAAACATCTTAAAGATATAGTGAGCCTTGGGATGAAACCGGATGTGGCAGCATACGGGATCATTTTTAATGAGTACTGTAAGATGAGAAAAGCGGATGGAGCTGTTTCCTTTTTGAGGGAAATGAGGATGAGAGGCCTAGAGCCAGGTGTTTCAAACTTCAATAGACTGCTAACCGTTCTTGTGGAAAGCGGGGACCTGGAAAGAGCCATTGTTCTTCTGAAGCAGATGCCACAAATGGGTTGCTCTCCAAATTTCTTTTCGTATAACACAGTGATAAGTAGTCTTTGTGATATGAGAGGTAGAATGGGGGTAGTTGAGGAGCTTGTTCGTGGCATGCTTCAGAATGGTCATGAGCTTGATACCGCCCTCTACAGTCGCGTGATTAAGGGTTATTGTGAGGATGGCAATGTGAAAATGGCAATGCAGGCTTTCTGTGCAGCACTGGATGCTAAATACATTATTAGTTTGGAGAGCTTTTCGGTTTTCGTTAAGGAGTTATGTGCGAAAGGGATGGTCGTTGAGGCTGAGAGGGTATTTGACGATATGCATAAGAGATGCACCGTGGCTGATGTGGATAATTACAGAAGGGTGCTAGATGAGCATTTTAGGGAGATAACTGAGGATACTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

312

Amino Acids

34.87

Weight (kDa)

6.13

Isoelectric Point (pI)

26.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 5 - 38 3.1e-13 PPR repeat
PPR_2 PF13041 9 - 57 1.5e-22 PPR repeat family
PPR_3 PF13812 10 - 54 2.5e-12 Pentatricopeptide repeat domain
PPR PF01535 12 - 42 1.8e-11 PPR repeat
PPR_1 PF12854 41 - 73 3.7e-13 PPR repeat
PPR_2 PF13041 44 - 92 8.9e-12 PPR repeat family
PPR PF01535 47 - 77 1.1e-06 PPR repeat
PPR_long PF17177 66 - 176 6.4e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 80 - 128 4.5e-08 PPR repeat family
PPR_2 PF13041 114 - 150 3.7e-06 PPR repeat family
PPR_3 PF13812 138 - 195 1.3e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 149 - 198 3.9e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 681
AciI CCGC 3 cut(s) 395, 492, 666
AclWI GGATC 1 cut(s) 368
AcsI RAATTY 1 cut(s) 553
AcuI CTGAAG 2 cut(s) 542, 626
AfaI GTAC 2 cut(s) 31, 377
AfiI CCNNNNNNNGG 2 cut(s) 417, 539
AgsI TTSAA 3 cut(s) 265, 456, 463
AjnI CCWGG 3 cut(s) 25, 445, 498
AjuI GAANNNNNNNTTGG 4 cut(s) 62, 94, 544, 576
AluBI AGCT 4 cut(s) 404, 625, 658, 777
AluI AGCT 4 cut(s) 404, 625, 658, 777
AlwI GGATC 1 cut(s) 368
AoxI GGCC 2 cut(s) 287, 436
ApeKI GCWGC 3 cut(s) 299, 350, 740
ApoI RAATTY 1 cut(s) 553
AspS9I GGNCC 1 cut(s) 496
AsuHPI GGTGA 2 cut(s) 200, 217
AvaII GGWCC 1 cut(s) 496
BbvI GCAGC 3 cut(s) 286, 362, 752
BccI CCATC 5 cut(s) 50, 196, 392, 698, 808
BceAI ACGGC 1 cut(s) 270
BciT130I CCWGG 3 cut(s) 27, 447, 500
BciVI GTATCC 1 cut(s) 919
BfaI CTAG 2 cut(s) 440, 896
BfmI CTRYAG 1 cut(s) 673
BfuI GTATCC 1 cut(s) 919
BisI GCNGC 3 cut(s) 300, 351, 741
BlsI GCNGC 3 cut(s) 301, 352, 742
BmcAI AGTACT 1 cut(s) 377
Bme1390I CCNGG 3 cut(s) 27, 447, 500
Bme18I GGWCC 1 cut(s) 496
BmgT120I GGNCC 1 cut(s) 496
BmiI GGNNCC 1 cut(s) 497
BmrFI CCNGG 3 cut(s) 27, 447, 500
BmsI GCATC 3 cut(s) 519, 739, 848
BsaJI CCNNGG 2 cut(s) 328, 864
BsaWI WCCGGW 1 cut(s) 340
Bsc4I CCNNNNNNNGG 2 cut(s) 417, 539
Bse1I ACTGG 1 cut(s) 750
Bse3DI GCAATG 4 cut(s) 201, 276, 715, 729
BseBI CCWGG 3 cut(s) 27, 447, 500
BseDI CCNNGG 2 cut(s) 328, 864
BseGI GGATG 8 cut(s) 207, 339, 349, 403, 435, 709, 754, 819
BseLI CCNNNNNNNGG 2 cut(s) 417, 539
BseMI GCAATG 4 cut(s) 201, 276, 715, 729
BseMII CTCAG 2 cut(s) 819, 912
BseNI ACTGG 1 cut(s) 750
BseRI GAGGAG 2 cut(s) 98, 635
BseXI GCAGC 3 cut(s) 286, 362, 752
BsgI GTGCAG 1 cut(s) 759
Bsh1236I CGCG 1 cut(s) 681
BshFI GGCC 2 cut(s) 289, 438
BsiSI CCGG 1 cut(s) 341
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 2 cut(s) 417, 539
BsmFI GGGAC 1 cut(s) 509
BsnI GGCC 2 cut(s) 289, 438
Bsp143I GATC 1 cut(s) 360
BspACI CCGC 3 cut(s) 395, 492, 666
BspANI GGCC 2 cut(s) 289, 438
BspCNI CTCAG 2 cut(s) 820, 913
BspFNI CGCG 1 cut(s) 681
BspHI TCATGA 1 cut(s) 652
BspLI GGNNCC 1 cut(s) 497
BspPI GGATC 1 cut(s) 368
BsrDI GCAATG 4 cut(s) 201, 276, 715, 729
BsrI ACTGG 1 cut(s) 750
BssECI CCNNGG 2 cut(s) 328, 864
BssMI GATC 1 cut(s) 360
BssT1I CCWWGG 1 cut(s) 328
Bst2UI CCWGG 3 cut(s) 27, 447, 500
Bst4CI ACNGT 6 cut(s) 34, 380, 478, 574, 677, 865
BstAPI GCANNNNNTGC 1 cut(s) 749
BstC8I GCNNGC 3 cut(s) 23, 638, 729
BstDEI CTNAG 2 cut(s) 828, 921
BstDSI CCRYGG 1 cut(s) 864
BstENI CCTNNNNNAGG 1 cut(s) 415
BstF5I GGATG 8 cut(s) 207, 339, 349, 403, 435, 709, 754, 819
BstFNI CGCG 1 cut(s) 681
BstKTI GATC 1 cut(s) 363
BstMBI GATC 1 cut(s) 360
BstMWI GCNNNNNNNGC 5 cut(s) 401, 728, 737, 749, 901
BstNI CCWGG 3 cut(s) 27, 447, 500
BstNSI RCATGY 1 cut(s) 640
BstSCI CCNGG 3 cut(s) 25, 445, 498
BstSFI CTRYAG 1 cut(s) 673
BstUI CGCG 1 cut(s) 681
BstV1I GCAGC 3 cut(s) 286, 362, 752
BsuI GTATCC 1 cut(s) 919
BsuRI GGCC 2 cut(s) 289, 438
BtgI CCRYGG 1 cut(s) 864
BtgZI GCGATG 1 cut(s) 104
BtsCI GGATG 8 cut(s) 207, 339, 349, 403, 435, 709, 754, 819
BtsIMutI CAGTG 2 cut(s) 579, 743
Cac8I GCNNGC 3 cut(s) 23, 638, 729
CciI TCATGA 1 cut(s) 652
Cfr13I GGNCC 1 cut(s) 496
CseI GACGC 1 cut(s) 305
Csp6I GTAC 2 cut(s) 30, 376
CviAII CATG 3 cut(s) 161, 637, 653
CviQI GTAC 2 cut(s) 30, 376
DdeI CTNAG 2 cut(s) 828, 921
DpnI GATC 1 cut(s) 362
DpnII GATC 1 cut(s) 360
Eco130I CCWWGG 1 cut(s) 328
Eco147I AGGCCT 2 cut(s) 289, 438
Eco47I GGWCC 1 cut(s) 496
Eco57I CTGAAG 2 cut(s) 542, 626
EcoNI CCTNNNNNAGG 1 cut(s) 415
EcoO109I RGGNCCY 1 cut(s) 496
EcoRII CCWGG 3 cut(s) 25, 445, 498
EcoT14I CCWWGG 1 cut(s) 328
EcoT22I ATGCAT 1 cut(s) 852
ErhI CCWWGG 1 cut(s) 328
FaeI CATG 3 cut(s) 164, 640, 656
FalI AAGNNNNNCTT 1 cut(s) 37
FaqI GGGAC 1 cut(s) 509
FatI CATG 3 cut(s) 160, 636, 652
FauI CCCGC 1 cut(s) 485
Fnu4HI GCNGC 3 cut(s) 300, 351, 741
FokI GGATG 8 cut(s) 214, 346, 356, 410, 442, 716, 761, 826
Fsp4HI GCNGC 3 cut(s) 300, 351, 741
FspBI CTAG 2 cut(s) 440, 896
GluI GCNGC 3 cut(s) 300, 351, 741
HaeIII GGCC 2 cut(s) 289, 438
HapII CCGG 1 cut(s) 341
HgaI GACGC 1 cut(s) 305
Hin1II CATG 3 cut(s) 164, 640, 656
HpaII CCGG 1 cut(s) 341
HphI GGTGA 2 cut(s) 200, 217
Hpy188I TCNGA 2 cut(s) 522, 645
Hpy188III TCNNGA 2 cut(s) 262, 653
Hpy99I CGWCG 1 cut(s) 299
HpyAV CCTTC 2 cut(s) 226, 882
HpyCH4III ACNGT 6 cut(s) 34, 380, 478, 574, 677, 865
HpyCH4V TGCA 6 cut(s) 276, 281, 727, 740, 850, 861
HpyF10VI GCNNNNNNNGC 5 cut(s) 401, 728, 737, 749, 901
HpyF3I CTNAG 2 cut(s) 828, 921
Hsp92II CATG 3 cut(s) 164, 640, 656
Kzo9I GATC 1 cut(s) 360
LmnI GCTCC 2 cut(s) 401, 622
Lsp1109I GCAGC 3 cut(s) 286, 362, 752
LweI GCATC 3 cut(s) 519, 739, 848
MaeI CTAG 2 cut(s) 440, 896
MaeIII GTNAC 1 cut(s) 34
MalI GATC 1 cut(s) 362
MboI GATC 1 cut(s) 360
MboII GAAGA 1 cut(s) 509
MluCI AATT 3 cut(s) 156, 553, 880
MmeI TCCRAC 1 cut(s) 60
Mph1103I ATGCAT 1 cut(s) 852
MseI TTAA 6 cut(s) 155, 221, 312, 369, 687, 792
MspI CCGG 1 cut(s) 341
MspR9I CCNGG 3 cut(s) 27, 447, 500
MvaI CCWGG 3 cut(s) 27, 447, 500
MvnI CGCG 1 cut(s) 681
MwoI GCNNNNNNNGC 5 cut(s) 401, 728, 737, 749, 901
NdeII GATC 1 cut(s) 360
NlaIII CATG 3 cut(s) 164, 640, 656
NlaIV GGNNCC 1 cut(s) 497
NsiI ATGCAT 1 cut(s) 852
NspI RCATGY 1 cut(s) 640
PaeI GCATGC 1 cut(s) 640
PagI TCATGA 1 cut(s) 652
PceI AGGCCT 2 cut(s) 289, 438
PkrI GCNGC 3 cut(s) 301, 352, 742
PpuMI RGGWCCY 1 cut(s) 496
Psp5II RGGWCCY 1 cut(s) 496
Psp6I CCWGG 3 cut(s) 25, 445, 498
PspGI CCWGG 3 cut(s) 25, 445, 498
PspN4I GGNNCC 1 cut(s) 497
PspPI GGNCC 1 cut(s) 496
PspPPI RGGWCCY 1 cut(s) 496
RsaI GTAC 2 cut(s) 31, 377
RsaNI GTAC 2 cut(s) 30, 376
SaqAI TTAA 6 cut(s) 155, 221, 312, 369, 687, 792
SatI GCNGC 3 cut(s) 300, 351, 741
Sau3AI GATC 1 cut(s) 360
Sau96I GGNCC 1 cut(s) 496
ScaI AGTACT 1 cut(s) 377
ScrFI CCNGG 3 cut(s) 27, 447, 500
SetI ASST 8 cut(s) 221, 406, 451, 501, 604, 627, 660, 779
SfaNI GCATC 3 cut(s) 519, 739, 848
SfcI CTRYAG 1 cut(s) 673
SinI GGWCC 1 cut(s) 496
SphI GCATGC 1 cut(s) 640
Sse9I AATT 3 cut(s) 156, 553, 880
SseBI AGGCCT 2 cut(s) 289, 438
SsiI CCGC 3 cut(s) 395, 492, 666
SspMI CTAG 2 cut(s) 440, 896
StuI AGGCCT 2 cut(s) 289, 438
StyD4I CCNGG 3 cut(s) 25, 445, 498
StyI CCWWGG 1 cut(s) 328
TaaI ACNGT 6 cut(s) 34, 380, 478, 574, 677, 865
TasI AATT 3 cut(s) 156, 553, 880
TatI WGTACW 1 cut(s) 375
Tru1I TTAA 6 cut(s) 155, 221, 312, 369, 687, 792
Tru9I TTAA 6 cut(s) 155, 221, 312, 369, 687, 792
TscAI CASTG 2 cut(s) 579, 750
TseI GCWGC 3 cut(s) 299, 350, 740
TspDTI ATGAA 3 cut(s) 107, 149, 350
TspRI CASTG 2 cut(s) 579, 750
VpaK11BI GGWCC 1 cut(s) 496
XagI CCTNNNNNAGG 1 cut(s) 415
XapI RAATTY 1 cut(s) 553
XceI RCATGY 1 cut(s) 640
XspI CTAG 2 cut(s) 440, 896
ZrmI AGTACT 1 cut(s) 377
Zsp2I ATGCAT 1 cut(s) 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.