Rh6AG504400

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0010419
Physical Location & Seq
Forward (+)
67670226 .. 67674242
4017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG504400.1

Sequence Viewer

Length: 927 bp
ATGAAAGCAGCACTATCCACACTGATCAGCTCCAAGACTCTCAGCCCTCACCCCTCTTCTTCTTCTTTCTCTCGCGCCTTTTGGCTTCAACCAAAGACCCTCTCCAACTCACCCTCCTTCATTACCCTTATCCCCTCCAAGACCCTCACCACCAACAATTACTCCCGTCTCCGCCTCAATCATCGCAAGTCCCTCACCTCGTCGATAATGGCCTCCGCTTTCAAGCCCGAAGAAGCCAGGGTCCCACCTGCCCTTCCCTTGCCCACTCCTCCGGTCAACAAGTTTAAGATTGGACTGTGCCAATTGTCGGTGACGGCCGATAAGGAGAGGAACATTGCTCATGCTCGCAAGGCCATCGAGCAGGCTGCCGCACAGGGTGCTCAGCTGGTTCTCCTACCTGAAATATGGAATGGCCCATATTCAAATGATAGCTTCCCAGTGTTTGCTGAGGATATTGACGCTGGTGGGGATGCATCTCCTTCGACAGCCATGCTTTCTGAAGTTGCTCAGCGCCTGAAAATCACCATTGTTGGTGGATCTATATCAGAGCGTTCCGGTGATCGGCTGTACAATACTTGCTGTGTCTTTGGTACTGATGGAAAGCTGAAAGCTAAACATAGGAAGATACACCTCTTTGATATTGATATCCCTGGGAAGATTACCTTTATTGAATCAAAGACTCTTACAGCAGGGCAAACTCCTACCATTGTGGACACAGAAGTTGGGCGTATTGGCATAGGCATTTGTTATGACATTAGGTTCCAGGAACTAGCCATGATCTATGGAGCAAGAGGTGCTCACTTGATATGCTATCCTGGGGCTTTTAACATGACCACTGGGCCATTGCATTGGGAATTGTTGCAGAGAGCAAGGGCTACAGATAACCAGGTACTCTATCCTACATCCTGTATGAGATATGTATCCTGA

Protein Analysis

308

Amino Acids

33.65

Weight (kDa)

9.37

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 96 - 297 8.9e-52 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 256
Acc36I ACCTGC 1 cut(s) 256
AccII CGCG 1 cut(s) 75
AciI CCGC 3 cut(s) 172, 216, 369
AclWI GGATC 1 cut(s) 544
AcoI YGGCCR 1 cut(s) 315
AcuI CTGAAG 1 cut(s) 519
AdeI CACNNNGTG 1 cut(s) 377
AfaI GTAC 3 cut(s) 569, 592, 891
AfiI CCNNNNNNNGG 2 cut(s) 307, 561
AgsI TTSAA 4 cut(s) 89, 223, 423, 671
AjnI CCWGG 5 cut(s) 236, 649, 762, 814, 885
AluBI AGCT 5 cut(s) 30, 385, 432, 604, 611
AluI AGCT 5 cut(s) 30, 385, 432, 604, 611
Alw21I GWGCWC 2 cut(s) 382, 799
Alw26I GTCTC 1 cut(s) 173
AlwI GGATC 1 cut(s) 544
AlwNI CAGNNNCTG 1 cut(s) 514
AoxI GGCC 5 cut(s) 210, 315, 351, 412, 839
ApeKI GCWGC 2 cut(s) 8, 365
AspLEI GCGC 2 cut(s) 77, 513
AspS9I GGNCC 3 cut(s) 241, 413, 839
AsuHPI GGTGA 7 cut(s) 41, 102, 139, 187, 322, 514, 569
AvaII GGWCC 1 cut(s) 241
Bbv12I GWGCWC 2 cut(s) 382, 799
BbvCI CCTCAGC 1 cut(s) 447
BbvI GCAGC 2 cut(s) 20, 352
BccI CCATC 2 cut(s) 362, 590
BceAI ACGGC 1 cut(s) 330
BcgI CGANNNNNNTGC 8 cut(s) 337, 347, 371, 381, 462, 472, 496, 506
BciT130I CCWGG 5 cut(s) 238, 651, 764, 816, 887
BclI TGATCA 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 173
BfaI CTAG 1 cut(s) 770
BfmI CTRYAG 1 cut(s) 876
BfoI RGCGCY 1 cut(s) 514
BfuAI ACCTGC 1 cut(s) 256
BisI GCNGC 3 cut(s) 9, 366, 369
BlpI GCTNAGC 2 cut(s) 381, 507
BlsI GCNGC 3 cut(s) 10, 367, 370
Bme1390I CCNGG 5 cut(s) 238, 651, 764, 816, 887
Bme18I GGWCC 1 cut(s) 241
BmgT120I GGNCC 3 cut(s) 241, 413, 839
BmiI GGNNCC 3 cut(s) 242, 243, 761
BmrFI CCNGG 5 cut(s) 238, 651, 764, 816, 887
BmrI ACTGGG 2 cut(s) 431, 846
BmsI GCATC 2 cut(s) 460, 482
BmuI ACTGGG 2 cut(s) 431, 846
Bpu10I CCTNAGC 1 cut(s) 447
Bpu1102I GCTNAGC 2 cut(s) 381, 507
BsaBI GATNNNNATC 2 cut(s) 541, 919
BsaJI CCNNGG 4 cut(s) 237, 649, 650, 815
BsaWI WCCGGW 2 cut(s) 271, 554
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 146, 176
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 561
Bse1I ACTGG 2 cut(s) 437, 841
Bse3DI GCAATG 2 cut(s) 333, 842
Bse8I GATNNNNATC 2 cut(s) 541, 919
BseBI CCWGG 5 cut(s) 238, 651, 764, 816, 887
BseDI CCNNGG 4 cut(s) 237, 649, 650, 815
BseGI GGATG 2 cut(s) 475, 902
BseJI GATNNNNATC 2 cut(s) 541, 919
BseLI CCNNNNNNNGG 2 cut(s) 307, 561
BseMI GCAATG 2 cut(s) 333, 842
BseMII CTCAG 4 cut(s) 55, 395, 438, 521
BseNI ACTGG 2 cut(s) 437, 841
BseRI GAGGAG 1 cut(s) 258
BseX3I CGGCCG 1 cut(s) 315
BseXI GCAGC 2 cut(s) 20, 352
Bsh1236I CGCG 1 cut(s) 75
Bsh1285I CGRYCG 1 cut(s) 318
BshFI GGCC 5 cut(s) 212, 317, 353, 414, 841
BsiEI CGRYCG 1 cut(s) 318
BsiHKAI GWGCWC 2 cut(s) 382, 799
BsiSI CCGG 2 cut(s) 272, 555
BslFI GGGAC 2 cut(s) 175, 227
BslI CCNNNNNNNGG 2 cut(s) 307, 561
BsmAI GTCTC 1 cut(s) 173
BsmBI CGTCTC 1 cut(s) 173
BsmFI GGGAC 2 cut(s) 175, 227
BsnI GGCC 5 cut(s) 212, 317, 353, 414, 841
Bsp1286I GDGCHC 2 cut(s) 382, 799
Bsp1407I TGTACA 1 cut(s) 567
Bsp143I GATC 4 cut(s) 24, 536, 559, 777
Bsp1720I GCTNAGC 2 cut(s) 381, 507
BspACI CCGC 3 cut(s) 172, 216, 369
BspANI GGCC 5 cut(s) 212, 317, 353, 414, 841
BspCNI CTCAG 4 cut(s) 54, 394, 439, 520
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 3 cut(s) 242, 243, 761
BspMI ACCTGC 1 cut(s) 256
BspPI GGATC 1 cut(s) 544
BsrDI GCAATG 2 cut(s) 333, 842
BsrGI TGTACA 1 cut(s) 567
BsrI ACTGG 2 cut(s) 437, 841
BssECI CCNNGG 4 cut(s) 237, 649, 650, 815
BssMI GATC 4 cut(s) 24, 536, 559, 777
Bst2UI CCWGG 5 cut(s) 238, 651, 764, 816, 887
Bst4CI ACNGT 1 cut(s) 297
Bst6I CTCTTC 1 cut(s) 61
BstAPI GCANNNNNTGC 2 cut(s) 377, 794
BstAUI TGTACA 1 cut(s) 567
BstC8I GCNNGC 2 cut(s) 346, 363
BstDEI CTNAG 4 cut(s) 41, 381, 447, 507
BstF5I GGATG 2 cut(s) 475, 902
BstFNI CGCG 1 cut(s) 75
BstH2I RGCGCY 1 cut(s) 514
BstHHI GCGC 2 cut(s) 77, 513
BstKTI GATC 4 cut(s) 27, 539, 562, 780
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 4 cut(s) 24, 536, 559, 777
BstMCI CGRYCG 1 cut(s) 318
BstMWI GCNNNNNNNGC 3 cut(s) 350, 377, 794
BstNI CCWGG 5 cut(s) 238, 651, 764, 816, 887
BstSCI CCNGG 5 cut(s) 236, 649, 762, 814, 885
BstSFI CTRYAG 1 cut(s) 876
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 2 cut(s) 20, 352
BstX2I RGATCY 1 cut(s) 536
BstXI CCANNNNNNTGG 1 cut(s) 849
BstYI RGATCY 1 cut(s) 536
BstZI CGGCCG 1 cut(s) 315
BsuRI GGCC 5 cut(s) 212, 317, 353, 414, 841
BtgZI GCGATG 1 cut(s) 167
BtsCI GGATG 2 cut(s) 475, 902
BtsIMutI CAGTG 3 cut(s) 20, 444, 834
BveI ACCTGC 1 cut(s) 256
Cac8I GCNNGC 2 cut(s) 346, 363
CaiI CAGNNNCTG 1 cut(s) 514
CfoI GCGC 2 cut(s) 77, 513
Cfr13I GGNCC 3 cut(s) 241, 413, 839
CseI GACGC 1 cut(s) 467
CsiI ACCWGGT 1 cut(s) 885
Csp6I GTAC 3 cut(s) 568, 591, 890
CviAII CATG 4 cut(s) 341, 490, 775, 829
CviQI GTAC 3 cut(s) 568, 591, 890
DdeI CTNAG 4 cut(s) 41, 381, 447, 507
DpnI GATC 4 cut(s) 26, 538, 561, 779
DpnII GATC 4 cut(s) 24, 536, 559, 777
DraIII CACNNNGTG 1 cut(s) 377
EaeI YGGCCR 1 cut(s) 315
EagI CGGCCG 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 61
EarI CTCTTC 1 cut(s) 61
EciI GGCGGA 1 cut(s) 161
EclXI CGGCCG 1 cut(s) 315
Eco32I GATATC 1 cut(s) 646
Eco47I GGWCC 1 cut(s) 241
Eco52I CGGCCG 1 cut(s) 315
Eco57I CTGAAG 1 cut(s) 519
EcoO109I RGGNCCY 1 cut(s) 241
EcoRII CCWGG 5 cut(s) 236, 649, 762, 814, 885
EcoRV GATATC 1 cut(s) 646
EcoT22I ATGCAT 1 cut(s) 475
Esp3I CGTCTC 1 cut(s) 173
FaeI CATG 4 cut(s) 344, 493, 778, 832
FalI AAGNNNNNCTT 2 cut(s) 647, 679
FaqI GGGAC 2 cut(s) 175, 227
FatI CATG 4 cut(s) 340, 489, 774, 828
FbaI TGATCA 1 cut(s) 24
Fnu4HI GCNGC 3 cut(s) 9, 366, 369
FokI GGATG 2 cut(s) 482, 889
Fsp4HI GCNGC 3 cut(s) 9, 366, 369
FspBI CTAG 1 cut(s) 770
GlaI GCGC 2 cut(s) 76, 512
GluI GCNGC 3 cut(s) 9, 366, 369
HaeII RGCGCY 1 cut(s) 514
HaeIII GGCC 5 cut(s) 212, 317, 353, 414, 841
HapII CCGG 2 cut(s) 272, 555
HgaI GACGC 1 cut(s) 467
HhaI GCGC 2 cut(s) 77, 513
Hin1II CATG 4 cut(s) 344, 493, 778, 832
Hin6I GCGC 2 cut(s) 75, 511
HinP1I GCGC 2 cut(s) 75, 511
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 3 cut(s) 37, 671, 679
HpaII CCGG 2 cut(s) 272, 555
HphI GGTGA 7 cut(s) 41, 102, 139, 187, 322, 514, 569
Hpy166II GTNNAC 2 cut(s) 277, 712
Hpy188I TCNGA 2 cut(s) 499, 547
Hpy188III TCNNGA 1 cut(s) 924
Hpy8I GTNNAC 2 cut(s) 277, 712
Hpy99I CGWCG 1 cut(s) 205
HpyAV CCTTC 3 cut(s) 127, 263, 489
HpyCH4III ACNGT 1 cut(s) 297
HpyCH4V TGCA 3 cut(s) 473, 847, 862
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 377, 794
HpyF3I CTNAG 4 cut(s) 41, 381, 447, 507
Hsp92II CATG 4 cut(s) 344, 493, 778, 832
HspAI GCGC 2 cut(s) 75, 511
KflI GGGWCCC 1 cut(s) 241
Ksp22I TGATCA 1 cut(s) 24
Kzo9I GATC 4 cut(s) 24, 536, 559, 777
LmnI GCTCC 2 cut(s) 35, 785
Lsp1109I GCAGC 2 cut(s) 20, 352
LweI GCATC 2 cut(s) 460, 482
MabI ACCWGGT 1 cut(s) 885
MaeI CTAG 1 cut(s) 770
MaeIII GTNAC 1 cut(s) 310
MalI GATC 4 cut(s) 26, 538, 561, 779
MboI GATC 4 cut(s) 24, 536, 559, 777
MboII GAAGA 6 cut(s) 48, 51, 54, 242, 634, 667
MfeI CAATTG 1 cut(s) 302
MflI RGATCY 1 cut(s) 536
MhlI GDGCHC 2 cut(s) 382, 799
MluCI AATT 3 cut(s) 157, 302, 854
MlyI GAGTC 2 cut(s) 31, 673
MmeI TCCRAC 1 cut(s) 129
Mph1103I ATGCAT 1 cut(s) 475
MseI TTAA 2 cut(s) 285, 825
MspA1I CMGCKG 1 cut(s) 385
MspI CCGG 2 cut(s) 272, 555
MspR9I CCNGG 5 cut(s) 238, 651, 764, 816, 887
MunI CAATTG 1 cut(s) 302
MvaI CCWGG 5 cut(s) 238, 651, 764, 816, 887
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 3 cut(s) 350, 377, 794
NdeII GATC 4 cut(s) 24, 536, 559, 777
NlaIII CATG 4 cut(s) 344, 493, 778, 832
NlaIV GGNNCC 3 cut(s) 242, 243, 761
NmuCI GTSAC 1 cut(s) 310
NsiI ATGCAT 1 cut(s) 475
PaqCI CACCTGC 1 cut(s) 256
PasI CCCWGGG 1 cut(s) 650
PfeI GAWTC 1 cut(s) 671
PfoI TCCNGGA 1 cut(s) 762
PkrI GCNGC 3 cut(s) 10, 367, 370
PleI GAGTC 2 cut(s) 31, 673
PpsI GAGTC 2 cut(s) 31, 673
PpuMI RGGWCCY 1 cut(s) 241
Psp5II RGGWCCY 1 cut(s) 241
Psp6I CCWGG 5 cut(s) 236, 649, 762, 814, 885
PspGI CCWGG 5 cut(s) 236, 649, 762, 814, 885
PspN4I GGNNCC 3 cut(s) 242, 243, 761
PspPI GGNCC 3 cut(s) 241, 413, 839
PspPPI RGGWCCY 1 cut(s) 241
PstNI CAGNNNCTG 1 cut(s) 514
PsuI RGATCY 1 cut(s) 536
PvuII CAGCTG 1 cut(s) 385
RsaI GTAC 3 cut(s) 569, 592, 891
RsaNI GTAC 3 cut(s) 568, 591, 890
SaqAI TTAA 2 cut(s) 285, 825
SatI GCNGC 3 cut(s) 9, 366, 369
Sau3AI GATC 4 cut(s) 24, 536, 559, 777
Sau96I GGNCC 3 cut(s) 241, 413, 839
SchI GAGTC 2 cut(s) 31, 673
ScrFI CCNGG 5 cut(s) 238, 651, 764, 816, 887
SduI GDGCHC 2 cut(s) 382, 799
SexAI ACCWGGT 1 cut(s) 885
SfaNI GCATC 2 cut(s) 460, 482
SfcI CTRYAG 1 cut(s) 876
SinI GGWCC 1 cut(s) 241
Sse9I AATT 3 cut(s) 157, 302, 854
SsiI CCGC 3 cut(s) 172, 216, 369
SspMI CTAG 1 cut(s) 770
StyD4I CCNGG 5 cut(s) 236, 649, 762, 814, 885
TaaI ACNGT 1 cut(s) 297
TaqI TCGA 3 cut(s) 203, 357, 482
TasI AATT 3 cut(s) 157, 302, 854
TatI WGTACW 1 cut(s) 567
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 1 cut(s) 671
Tru1I TTAA 2 cut(s) 285, 825
Tru9I TTAA 2 cut(s) 285, 825
TscAI CASTG 3 cut(s) 27, 444, 841
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 2 cut(s) 8, 365
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 2 cut(s) 17, 109
TspRI CASTG 3 cut(s) 27, 444, 841
VpaK11BI GGWCC 1 cut(s) 241
XspI CTAG 1 cut(s) 770
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.