Rh6DG506800

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0010419
Physical Location & Seq
Forward (+)
66069181 .. 66073064
3884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG506800.1

Sequence Viewer

Length: 1158 bp
ATGAAAGGAGCACTATCCACTCTGATCAGCTCCAGGACTCTGAGCCCTCCCCCCTCTCCTTCTTCTTCTTCTTTCGCTCGCTCCATTTGGCTTCAACCAAAGACCCTCTCCAACTCACCCTCCTTCATTACCCCTATACCCTCCAAGACCCTCACCACCAACACCAACACCAACAATTACTCCCGTCTCCGTCTCAATCATCGCAAGTCCCTCACCGCTAATTTCTCGTCGATAATGGCCTCCGCTTACAAGCCCGAAGAAGCCAGGGTCCCACCTGCCCTTCCCTTGCCCACTCCTCCCGTCAACAAGTTTAAGATTGGACTGTGTCAATTGTCGGTGACGGCCGATAAGGAGAGGAACATTGCTCATGCTCGCAAGGCCATCGAGCAGGCTGTCGAAAAGGGTGCTCAGCTGGTTCTCCTACCTGAAATATGGAATGGCCCGTATTCAAATGATAGCTTCCCAGTGTTTGCTGAGGATATTGACGCTGGTGGTGAAGCATCTCCTTCGACAGCCATGCTTTCTGAAGTTGCTCAGCGCCTGAAAATCACCATTGTTGGTGGATCTATATCAGAGCGTTCTGGTGATCAGCTGTACAATACTTGTTGTGTCTTTGGTACTGATGGAAAGCTGAAAGCTAAACATAGGAAGATACACCTCTTTGATATTGATATCCCTGGGAAGATTACCTTTATTGAATCAAAGACTCTTACTGCAGGGCAAACTCCTACCATTGTGGACACAGAAGTTGGGCGTATTGGCATAGGCATTTGTTATGACATTAGGTTCCAGGAACTAGCTATGATCTATGGAGCAAGAGGTGCTCACTTGATATGCTATCCTGGGGCTTTTAACATGACCACTGGGCCATTGCATTGGGAATTGTTGCAGAGAGCAAGGGCTACAGATAACCAGCTATATGTGGCAACTTGTTCACCTGCTCGAGATGCCGGAGCTGGTTATGTGGCATGGGGCCACTCCACCCTTGTCGGACCATTTGGAGAAGTGCTGGCAACTACAGAACATGAAGAGGCAATTATCATAGCTGAGGTTGATTATTCACTCCTTGAGCTTAGAAGAACAAATCTCCCTTTACTGAAGCAGCGGCGAGGAGATCTTTACCAGTTGGTAGATGTTCAGAGGTTGAATTCTAAATGA

Protein Analysis

385

Amino Acids

42.06

Weight (kDa)

8.92

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 105 - 357 9.2e-65 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 283, 946
Acc36I ACCTGC 2 cut(s) 283, 946
AciI CCGC 3 cut(s) 216, 243, 1105
AclWI GGATC 1 cut(s) 571
AcoI YGGCCR 1 cut(s) 342
AcsI RAATTY 1 cut(s) 1147
AcuI CTGAAG 2 cut(s) 546, 1118
AfaI GTAC 2 cut(s) 596, 619
AgsI TTSAA 4 cut(s) 95, 450, 698, 1147
AjnI CCWGG 5 cut(s) 32, 263, 676, 789, 841
Alw21I GWGCWC 3 cut(s) 13, 409, 826
Alw26I GTCTC 2 cut(s) 191, 197
AlwI GGATC 1 cut(s) 571
AlwNI CAGNNNCTG 1 cut(s) 541
Ama87I CYCGRG 1 cut(s) 942
AoxI GGCC 6 cut(s) 237, 342, 378, 439, 866, 973
ApeKI GCWGC 1 cut(s) 1102
ApoI RAATTY 1 cut(s) 1147
AspLEI GCGC 1 cut(s) 540
AspS9I GGNCC 5 cut(s) 268, 440, 866, 973, 992
AsuHPI GGTGA 8 cut(s) 108, 145, 205, 349, 506, 541, 596, 927
AvaI CYCGRG 1 cut(s) 942
AvaII GGWCC 2 cut(s) 268, 992
BanII GRGCYC 1 cut(s) 47
Bbv12I GWGCWC 3 cut(s) 13, 409, 826
BbvCI CCTCAGC 2 cut(s) 474, 1047
BbvI GCAGC 1 cut(s) 1114
BccI CCATC 2 cut(s) 389, 617
BceAI ACGGC 1 cut(s) 357
BcgI CGANNNNNNTGC 8 cut(s) 364, 386, 398, 420, 489, 499, 523, 533
BciT130I CCWGG 5 cut(s) 34, 265, 678, 791, 843
BclI TGATCA 2 cut(s) 24, 586
BcoDI GTCTC 2 cut(s) 191, 197
BfaI CTAG 1 cut(s) 797
BfmI CTRYAG 3 cut(s) 714, 903, 1017
BfoI RGCGCY 1 cut(s) 541
BfuAI ACCTGC 2 cut(s) 283, 946
BglII AGATCT 1 cut(s) 1114
BisI GCNGC 2 cut(s) 1103, 1106
BlpI GCTNAGC 2 cut(s) 408, 534
BlsI GCNGC 2 cut(s) 1104, 1107
Bme1390I CCNGG 5 cut(s) 34, 265, 678, 791, 843
Bme18I GGWCC 2 cut(s) 268, 992
BmeT110I CYCGRG 1 cut(s) 942
BmgT120I GGNCC 5 cut(s) 268, 440, 866, 973, 992
BmiI GGNNCC 4 cut(s) 269, 270, 788, 974
BmrFI CCNGG 5 cut(s) 34, 265, 678, 791, 843
BmrI ACTGGG 2 cut(s) 458, 873
BmsI GCATC 2 cut(s) 509, 937
BmuI ACTGGG 2 cut(s) 458, 873
BpmI CTGGAG 1 cut(s) 16
Bpu10I CCTNAGC 2 cut(s) 474, 1047
Bpu1102I GCTNAGC 2 cut(s) 408, 534
BpuEI CTTGAG 1 cut(s) 1088
BsaBI GATNNNNATC 1 cut(s) 568
BsaJI CCNNGG 4 cut(s) 264, 676, 677, 842
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 164, 194
Bse1I ACTGG 3 cut(s) 464, 868, 1123
Bse3DI GCAATG 2 cut(s) 360, 869
Bse8I GATNNNNATC 1 cut(s) 568
BseBI CCWGG 5 cut(s) 34, 265, 678, 791, 843
BseDI CCNNGG 4 cut(s) 264, 676, 677, 842
BseJI GATNNNNATC 1 cut(s) 568
BseMI GCAATG 2 cut(s) 360, 869
BseMII CTCAG 5 cut(s) 32, 422, 465, 548, 1038
BseNI ACTGG 3 cut(s) 464, 868, 1123
BseRI GAGGAG 2 cut(s) 285, 1125
BseX3I CGGCCG 1 cut(s) 342
BseXI GCAGC 1 cut(s) 1114
Bsh1285I CGRYCG 1 cut(s) 345
BshFI GGCC 6 cut(s) 239, 344, 380, 441, 868, 975
BsiEI CGRYCG 1 cut(s) 345
BsiHKAI GWGCWC 3 cut(s) 13, 409, 826
BsiHKCI CYCGRG 1 cut(s) 942
BsiSI CCGG 1 cut(s) 951
BslFI GGGAC 2 cut(s) 193, 254
BsmAI GTCTC 2 cut(s) 191, 197
BsmBI CGTCTC 2 cut(s) 191, 197
BsmFI GGGAC 2 cut(s) 193, 254
BsnI GGCC 6 cut(s) 239, 344, 380, 441, 868, 975
BsoBI CYCGRG 1 cut(s) 942
Bsp1286I GDGCHC 4 cut(s) 13, 47, 409, 826
Bsp1407I TGTACA 1 cut(s) 594
Bsp143I GATC 5 cut(s) 24, 563, 586, 804, 1114
Bsp1720I GCTNAGC 2 cut(s) 408, 534
BspACI CCGC 3 cut(s) 216, 243, 1105
BspANI GGCC 6 cut(s) 239, 344, 380, 441, 868, 975
BspCNI CTCAG 5 cut(s) 33, 421, 466, 547, 1039
BspLI GGNNCC 4 cut(s) 269, 270, 788, 974
BspMAI CTGCAG 1 cut(s) 718
BspMI ACCTGC 2 cut(s) 283, 946
BspPI GGATC 1 cut(s) 571
BsrDI GCAATG 2 cut(s) 360, 869
BsrGI TGTACA 1 cut(s) 594
BsrI ACTGG 3 cut(s) 464, 868, 1123
BssECI CCNNGG 4 cut(s) 264, 676, 677, 842
BssMI GATC 5 cut(s) 24, 563, 586, 804, 1114
Bst2UI CCWGG 5 cut(s) 34, 265, 678, 791, 843
Bst4CI ACNGT 1 cut(s) 324
Bst6I CTCTTC 1 cut(s) 1023
BstAPI GCANNNNNTGC 1 cut(s) 821
BstAUI TGTACA 1 cut(s) 594
BstC8I GCNNGC 4 cut(s) 79, 373, 390, 1011
BstDEI CTNAG 6 cut(s) 41, 408, 474, 534, 1047, 1073
BstH2I RGCGCY 1 cut(s) 541
BstHHI GCGC 1 cut(s) 540
BstKTI GATC 5 cut(s) 27, 566, 589, 807, 1117
BstMAI GTCTC 2 cut(s) 191, 197
BstMBI GATC 5 cut(s) 24, 563, 586, 804, 1114
BstMCI CGRYCG 1 cut(s) 345
BstMWI GCNNNNNNNGC 3 cut(s) 377, 821, 947
BstNI CCWGG 5 cut(s) 34, 265, 678, 791, 843
BstSCI CCNGG 5 cut(s) 32, 263, 676, 789, 841
BstSFI CTRYAG 3 cut(s) 714, 903, 1017
BstV1I GCAGC 1 cut(s) 1114
BstX2I RGATCY 2 cut(s) 563, 1114
BstXI CCANNNNNNTGG 1 cut(s) 876
BstYI RGATCY 2 cut(s) 563, 1114
BstZI CGGCCG 1 cut(s) 342
BsuRI GGCC 6 cut(s) 239, 344, 380, 441, 868, 975
BtgZI GCGATG 1 cut(s) 185
BtsIMutI CAGTG 2 cut(s) 471, 861
BveI ACCTGC 2 cut(s) 283, 946
Cac8I GCNNGC 4 cut(s) 79, 373, 390, 1011
CaiI CAGNNNCTG 1 cut(s) 541
CfoI GCGC 1 cut(s) 540
Cfr13I GGNCC 5 cut(s) 268, 440, 866, 973, 992
CseI GACGC 1 cut(s) 494
Csp6I GTAC 2 cut(s) 595, 618
CviAII CATG 5 cut(s) 368, 517, 856, 969, 1025
CviQI GTAC 2 cut(s) 595, 618
DdeI CTNAG 6 cut(s) 41, 408, 474, 534, 1047, 1073
DpnI GATC 5 cut(s) 26, 565, 588, 806, 1116
DpnII GATC 5 cut(s) 24, 563, 586, 804, 1114
EaeI YGGCCR 1 cut(s) 342
EagI CGGCCG 1 cut(s) 342
Eam1104I CTCTTC 1 cut(s) 1023
EarI CTCTTC 1 cut(s) 1023
EclXI CGGCCG 1 cut(s) 342
Eco24I GRGCYC 1 cut(s) 47
Eco32I GATATC 1 cut(s) 673
Eco47I GGWCC 2 cut(s) 268, 992
Eco52I CGGCCG 1 cut(s) 342
Eco57I CTGAAG 2 cut(s) 546, 1118
Eco88I CYCGRG 1 cut(s) 942
EcoO109I RGGNCCY 1 cut(s) 268
EcoRI GAATTC 1 cut(s) 1147
EcoRII CCWGG 5 cut(s) 32, 263, 676, 789, 841
EcoRV GATATC 1 cut(s) 673
EcoT38I GRGCYC 1 cut(s) 47
Esp3I CGTCTC 2 cut(s) 191, 197
FaeI CATG 5 cut(s) 371, 520, 859, 972, 1028
FalI AAGNNNNNCTT 2 cut(s) 674, 706
FaqI GGGAC 2 cut(s) 193, 254
FatI CATG 5 cut(s) 367, 516, 855, 968, 1024
FbaI TGATCA 2 cut(s) 24, 586
Fnu4HI GCNGC 2 cut(s) 1103, 1106
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 2 cut(s) 1103, 1106
FspBI CTAG 1 cut(s) 797
GlaI GCGC 1 cut(s) 539
GluI GCNGC 2 cut(s) 1103, 1106
GsuI CTGGAG 1 cut(s) 16
HaeII RGCGCY 1 cut(s) 541
HaeIII GGCC 6 cut(s) 239, 344, 380, 441, 868, 975
HapII CCGG 1 cut(s) 951
HgaI GACGC 1 cut(s) 494
HhaI GCGC 1 cut(s) 540
Hin1II CATG 5 cut(s) 371, 520, 859, 972, 1028
Hin6I GCGC 1 cut(s) 538
HinP1I GCGC 1 cut(s) 538
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HinfI GANTC 3 cut(s) 37, 698, 706
HpaII CCGG 1 cut(s) 951
HphI GGTGA 8 cut(s) 108, 145, 205, 349, 506, 541, 596, 927
Hpy166II GTNNAC 3 cut(s) 304, 739, 935
Hpy188I TCNGA 6 cut(s) 24, 42, 526, 574, 992, 1140
Hpy188III TCNNGA 1 cut(s) 944
Hpy8I GTNNAC 3 cut(s) 304, 739, 935
Hpy99I CGWCG 1 cut(s) 232
HpyAV CCTTC 4 cut(s) 69, 133, 290, 516
HpyCH4III ACNGT 1 cut(s) 324
HpyCH4V TGCA 3 cut(s) 716, 874, 889
HpyF10VI GCNNNNNNNGC 3 cut(s) 377, 821, 947
HpyF3I CTNAG 6 cut(s) 41, 408, 474, 534, 1047, 1073
Hsp92II CATG 5 cut(s) 371, 520, 859, 972, 1028
HspAI GCGC 1 cut(s) 538
KflI GGGWCCC 1 cut(s) 268
Ksp22I TGATCA 2 cut(s) 24, 586
Kzo9I GATC 5 cut(s) 24, 563, 586, 804, 1114
LmnI GCTCC 5 cut(s) 8, 35, 86, 812, 953
Lsp1109I GCAGC 1 cut(s) 1114
LweI GCATC 2 cut(s) 509, 937
MaeI CTAG 1 cut(s) 797
MaeIII GTNAC 1 cut(s) 337
MalI GATC 5 cut(s) 26, 565, 588, 806, 1116
MboI GATC 5 cut(s) 24, 563, 586, 804, 1114
MboII GAAGA 8 cut(s) 54, 57, 60, 269, 661, 694, 1040, 1089
MfeI CAATTG 1 cut(s) 329
MflI RGATCY 2 cut(s) 563, 1114
MhlI GDGCHC 4 cut(s) 13, 47, 409, 826
MluCI AATT 6 cut(s) 175, 220, 329, 881, 1035, 1147
MlyI GAGTC 2 cut(s) 31, 700
MmeI TCCRAC 2 cut(s) 135, 970
MseI TTAA 2 cut(s) 312, 852
MspA1I CMGCKG 3 cut(s) 412, 592, 1105
MspI CCGG 1 cut(s) 951
MspR9I CCNGG 5 cut(s) 34, 265, 678, 791, 843
MunI CAATTG 1 cut(s) 329
MvaI CCWGG 5 cut(s) 34, 265, 678, 791, 843
MwoI GCNNNNNNNGC 3 cut(s) 377, 821, 947
NdeII GATC 5 cut(s) 24, 563, 586, 804, 1114
NlaIII CATG 5 cut(s) 371, 520, 859, 972, 1028
NlaIV GGNNCC 4 cut(s) 269, 270, 788, 974
NmuCI GTSAC 1 cut(s) 337
PaeR7I CTCGAG 1 cut(s) 942
PaqCI CACCTGC 2 cut(s) 283, 946
PasI CCCWGGG 1 cut(s) 677
PfeI GAWTC 1 cut(s) 698
PflFI GACNNNGTC 1 cut(s) 324
PfoI TCCNGGA 2 cut(s) 32, 789
PkrI GCNGC 2 cut(s) 1104, 1107
PleI GAGTC 2 cut(s) 31, 700
PpsI GAGTC 2 cut(s) 31, 700
PpuMI RGGWCCY 1 cut(s) 268
Psp5II RGGWCCY 1 cut(s) 268
Psp6I CCWGG 5 cut(s) 32, 263, 676, 789, 841
PspGI CCWGG 5 cut(s) 32, 263, 676, 789, 841
PspN4I GGNNCC 4 cut(s) 269, 270, 788, 974
PspPI GGNCC 5 cut(s) 268, 440, 866, 973, 992
PspPPI RGGWCCY 1 cut(s) 268
PstI CTGCAG 1 cut(s) 718
PstNI CAGNNNCTG 1 cut(s) 541
PsuI RGATCY 2 cut(s) 563, 1114
PsyI GACNNNGTC 1 cut(s) 324
PvuII CAGCTG 2 cut(s) 412, 592
RsaI GTAC 2 cut(s) 596, 619
RsaNI GTAC 2 cut(s) 595, 618
SaqAI TTAA 2 cut(s) 312, 852
SatI GCNGC 2 cut(s) 1103, 1106
Sau3AI GATC 5 cut(s) 24, 563, 586, 804, 1114
Sau96I GGNCC 5 cut(s) 268, 440, 866, 973, 992
SchI GAGTC 2 cut(s) 31, 700
ScrFI CCNGG 5 cut(s) 34, 265, 678, 791, 843
SduI GDGCHC 4 cut(s) 13, 47, 409, 826
SfaNI GCATC 2 cut(s) 509, 937
SfcI CTRYAG 3 cut(s) 714, 903, 1017
Sfr274I CTCGAG 1 cut(s) 942
SinI GGWCC 2 cut(s) 268, 992
SlaI CTCGAG 1 cut(s) 942
SmlI CTYRAG 2 cut(s) 942, 1067
SmoI CTYRAG 2 cut(s) 942, 1067
Sse9I AATT 6 cut(s) 175, 220, 329, 881, 1035, 1147
SsiI CCGC 3 cut(s) 216, 243, 1105
SspMI CTAG 1 cut(s) 797
StyD4I CCNGG 5 cut(s) 32, 263, 676, 789, 841
TaaI ACNGT 1 cut(s) 324
TaqI TCGA 5 cut(s) 230, 384, 396, 509, 943
TasI AATT 6 cut(s) 175, 220, 329, 881, 1035, 1147
TatI WGTACW 1 cut(s) 594
TauI GCSGC 1 cut(s) 1108
TfiI GAWTC 1 cut(s) 698
Tru1I TTAA 2 cut(s) 312, 852
Tru9I TTAA 2 cut(s) 312, 852
TscAI CASTG 2 cut(s) 471, 868
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 1 cut(s) 1102
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 3 cut(s) 17, 115, 1041
TspGWI ACGGA 1 cut(s) 179
TspRI CASTG 2 cut(s) 471, 868
Tth111I GACNNNGTC 1 cut(s) 324
VpaK11BI GGWCC 2 cut(s) 268, 992
XapI RAATTY 1 cut(s) 1147
XhoI CTCGAG 1 cut(s) 942
XspI CTAG 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.