Rh6BG515000

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0010419
Physical Location & Seq
Forward (+)
70920417 .. 70925124
4708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG515000.1

Sequence Viewer

Length: 951 bp
ATGAAAGCAGCACTATCCACTCTGATCAGCTCCAGGACTCTGAGCCCTCCCCCCTCTCCTTCTTCTTCTTCTTCTTCTTCTTTCGCTCGCTCCATTTGGCTTCAACCAAAGACCCTCTCCAACTCACCCTCCTTCATTACCCCTATACCCTCCAAGACCCTCACCACCAACAATTACTCCCGTCTCCGTCTCAATCATCGCAAGTCCCTCACCGCTAATTTCTCGTCGAAAATGGCCTCCACTTACAAGCCCGAAGAAGCCAGGGTCCCACCTGCCCTTCCCTTGCCCACTCCTCCCGTCAACAAGTTTAAGATTGGACTGTGCCAATTGTCGGTGACGGCCGATAAGGAGAGGAACATTGCTCATGCTCGCAAGGCCATCGAGCAGGCTGTCGAAAAGGGTGCTCAGCTGGTTCTCCTACCTGAAATATGGAATGGCCCGTATTCAAATGATAGCTTCCCAGTGTTTGCTGAGGATATTGACGCTGGTGGTGAAGCATCTCCTTCGACAGCCATGCTTTCTGAAGTTGCTCAGCGCCTGAAAATCACCATTGTTGGTGGATCTATATCAGAGCGTTCTGGTGATCAGCTGTACAATACTTGTTGTGTCTTTGGTACTGATGGAAAGCTGAAAGCTAAACATAGGAAGATACACCTCTTTGATATTGATATCCCTGGGAAGATTACCTTTATTGAATCAAAGACTCTTACAGCAGGGCAAACTCCTACCATTGTGGACACAGAAGTTGGGCGTATTGGCATAGGCATTTGTTATGACATTAGGTTCCAGGAACTAGCTATGATCTATGGAGCAAGAGGTGCTCACTTGATATGCTATCCTGGGGCTTTTAACATGACCACTGGGCCATTGCATTGGGAATTGTTGCAGAGAGCAAGGGCTACAGATAACCAGGTACTCTATCCTACATCCTGTATGAGATATGTATCCTGA

Protein Analysis

316

Amino Acids

34.5

Weight (kDa)

9.34

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 104 - 305 5.7e-52 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 280
Acc36I ACCTGC 1 cut(s) 280
AciI CCGC 1 cut(s) 213
AclWI GGATC 1 cut(s) 568
AcoI YGGCCR 1 cut(s) 339
AcuI CTGAAG 1 cut(s) 543
AfaI GTAC 3 cut(s) 593, 616, 915
AfiI CCNNNNNNNGG 1 cut(s) 331
AgsI TTSAA 3 cut(s) 104, 447, 695
AjnI CCWGG 6 cut(s) 32, 260, 673, 786, 838, 909
AluBI AGCT 7 cut(s) 30, 409, 456, 589, 628, 635, 797
AluI AGCT 7 cut(s) 30, 409, 456, 589, 628, 635, 797
Alw21I GWGCWC 2 cut(s) 406, 823
Alw26I GTCTC 2 cut(s) 188, 194
AlwI GGATC 1 cut(s) 568
AlwNI CAGNNNCTG 1 cut(s) 538
AoxI GGCC 5 cut(s) 234, 339, 375, 436, 863
ApeKI GCWGC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 537
AspS9I GGNCC 3 cut(s) 265, 437, 863
AsuHPI GGTGA 7 cut(s) 117, 154, 202, 346, 503, 538, 593
AvaII GGWCC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 47
Bbv12I GWGCWC 2 cut(s) 406, 823
BbvCI CCTCAGC 1 cut(s) 471
BbvI GCAGC 1 cut(s) 20
BccI CCATC 2 cut(s) 386, 614
BceAI ACGGC 1 cut(s) 354
BcgI CGANNNNNNTGC 8 cut(s) 361, 383, 395, 417, 486, 496, 520, 530
BciT130I CCWGG 6 cut(s) 34, 262, 675, 788, 840, 911
BclI TGATCA 2 cut(s) 24, 583
BcoDI GTCTC 2 cut(s) 188, 194
BfaI CTAG 1 cut(s) 794
BfmI CTRYAG 1 cut(s) 900
BfoI RGCGCY 1 cut(s) 538
BfuAI ACCTGC 1 cut(s) 280
BisI GCNGC 1 cut(s) 9
BlpI GCTNAGC 2 cut(s) 405, 531
BlsI GCNGC 1 cut(s) 10
Bme1390I CCNGG 6 cut(s) 34, 262, 675, 788, 840, 911
Bme18I GGWCC 1 cut(s) 265
BmgT120I GGNCC 3 cut(s) 265, 437, 863
BmiI GGNNCC 3 cut(s) 266, 267, 785
BmrFI CCNGG 6 cut(s) 34, 262, 675, 788, 840, 911
BmrI ACTGGG 2 cut(s) 455, 870
BmsI GCATC 1 cut(s) 506
BmuI ACTGGG 2 cut(s) 455, 870
BpmI CTGGAG 1 cut(s) 16
Bpu10I CCTNAGC 1 cut(s) 471
Bpu1102I GCTNAGC 2 cut(s) 405, 531
BsaBI GATNNNNATC 2 cut(s) 565, 943
BsaJI CCNNGG 4 cut(s) 261, 673, 674, 839
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 161, 191
Bsc4I CCNNNNNNNGG 1 cut(s) 331
Bse1I ACTGG 2 cut(s) 461, 865
Bse3DI GCAATG 2 cut(s) 357, 866
Bse8I GATNNNNATC 2 cut(s) 565, 943
BseBI CCWGG 6 cut(s) 34, 262, 675, 788, 840, 911
BseDI CCNNGG 4 cut(s) 261, 673, 674, 839
BseGI GGATG 1 cut(s) 926
BseJI GATNNNNATC 2 cut(s) 565, 943
BseLI CCNNNNNNNGG 1 cut(s) 331
BseMI GCAATG 2 cut(s) 357, 866
BseMII CTCAG 4 cut(s) 32, 419, 462, 545
BseNI ACTGG 2 cut(s) 461, 865
BseRI GAGGAG 1 cut(s) 282
BseX3I CGGCCG 1 cut(s) 339
BseXI GCAGC 1 cut(s) 20
Bsh1285I CGRYCG 1 cut(s) 342
BshFI GGCC 5 cut(s) 236, 341, 377, 438, 865
BsiEI CGRYCG 1 cut(s) 342
BsiHKAI GWGCWC 2 cut(s) 406, 823
BslFI GGGAC 2 cut(s) 190, 251
BslI CCNNNNNNNGG 1 cut(s) 331
BsmAI GTCTC 2 cut(s) 188, 194
BsmBI CGTCTC 2 cut(s) 188, 194
BsmFI GGGAC 2 cut(s) 190, 251
BsnI GGCC 5 cut(s) 236, 341, 377, 438, 865
Bsp1286I GDGCHC 3 cut(s) 47, 406, 823
Bsp1407I TGTACA 1 cut(s) 591
Bsp143I GATC 4 cut(s) 24, 560, 583, 801
Bsp1720I GCTNAGC 2 cut(s) 405, 531
BspACI CCGC 1 cut(s) 213
BspANI GGCC 5 cut(s) 236, 341, 377, 438, 865
BspCNI CTCAG 4 cut(s) 33, 418, 463, 544
BspLI GGNNCC 3 cut(s) 266, 267, 785
BspMI ACCTGC 1 cut(s) 280
BspPI GGATC 1 cut(s) 568
BsrDI GCAATG 2 cut(s) 357, 866
BsrGI TGTACA 1 cut(s) 591
BsrI ACTGG 2 cut(s) 461, 865
BssECI CCNNGG 4 cut(s) 261, 673, 674, 839
BssMI GATC 4 cut(s) 24, 560, 583, 801
Bst2UI CCWGG 6 cut(s) 34, 262, 675, 788, 840, 911
Bst4CI ACNGT 1 cut(s) 321
BstAPI GCANNNNNTGC 1 cut(s) 818
BstAUI TGTACA 1 cut(s) 591
BstC8I GCNNGC 3 cut(s) 88, 370, 387
BstDEI CTNAG 4 cut(s) 41, 405, 471, 531
BstF5I GGATG 1 cut(s) 926
BstH2I RGCGCY 1 cut(s) 538
BstHHI GCGC 1 cut(s) 537
BstKTI GATC 4 cut(s) 27, 563, 586, 804
BstMAI GTCTC 2 cut(s) 188, 194
BstMBI GATC 4 cut(s) 24, 560, 583, 801
BstMCI CGRYCG 1 cut(s) 342
BstMWI GCNNNNNNNGC 2 cut(s) 374, 818
BstNI CCWGG 6 cut(s) 34, 262, 675, 788, 840, 911
BstSCI CCNGG 6 cut(s) 32, 260, 673, 786, 838, 909
BstSFI CTRYAG 1 cut(s) 900
BstV1I GCAGC 1 cut(s) 20
BstX2I RGATCY 1 cut(s) 560
BstXI CCANNNNNNTGG 1 cut(s) 873
BstYI RGATCY 1 cut(s) 560
BstZI CGGCCG 1 cut(s) 339
BsuRI GGCC 5 cut(s) 236, 341, 377, 438, 865
BtgZI GCGATG 1 cut(s) 182
BtsCI GGATG 1 cut(s) 926
BtsIMutI CAGTG 2 cut(s) 468, 858
BveI ACCTGC 1 cut(s) 280
Cac8I GCNNGC 3 cut(s) 88, 370, 387
CaiI CAGNNNCTG 1 cut(s) 538
CfoI GCGC 1 cut(s) 537
Cfr13I GGNCC 3 cut(s) 265, 437, 863
CseI GACGC 1 cut(s) 491
CsiI ACCWGGT 1 cut(s) 909
Csp6I GTAC 3 cut(s) 592, 615, 914
CviAII CATG 3 cut(s) 365, 514, 853
CviQI GTAC 3 cut(s) 592, 615, 914
DdeI CTNAG 4 cut(s) 41, 405, 471, 531
DpnI GATC 4 cut(s) 26, 562, 585, 803
DpnII GATC 4 cut(s) 24, 560, 583, 801
EaeI YGGCCR 1 cut(s) 339
EagI CGGCCG 1 cut(s) 339
EclXI CGGCCG 1 cut(s) 339
Eco24I GRGCYC 1 cut(s) 47
Eco32I GATATC 1 cut(s) 670
Eco47I GGWCC 1 cut(s) 265
Eco52I CGGCCG 1 cut(s) 339
Eco57I CTGAAG 1 cut(s) 543
EcoO109I RGGNCCY 1 cut(s) 265
EcoRII CCWGG 6 cut(s) 32, 260, 673, 786, 838, 909
EcoRV GATATC 1 cut(s) 670
EcoT38I GRGCYC 1 cut(s) 47
Esp3I CGTCTC 2 cut(s) 188, 194
FaeI CATG 3 cut(s) 368, 517, 856
FalI AAGNNNNNCTT 2 cut(s) 671, 703
FaqI GGGAC 2 cut(s) 190, 251
FatI CATG 3 cut(s) 364, 513, 852
FbaI TGATCA 2 cut(s) 24, 583
Fnu4HI GCNGC 1 cut(s) 9
FokI GGATG 1 cut(s) 913
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 1 cut(s) 9
FspBI CTAG 1 cut(s) 794
GlaI GCGC 1 cut(s) 536
GluI GCNGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 16
HaeII RGCGCY 1 cut(s) 538
HaeIII GGCC 5 cut(s) 236, 341, 377, 438, 865
HgaI GACGC 1 cut(s) 491
HhaI GCGC 1 cut(s) 537
Hin1II CATG 3 cut(s) 368, 517, 856
Hin6I GCGC 1 cut(s) 535
HinP1I GCGC 1 cut(s) 535
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 3 cut(s) 37, 695, 703
HphI GGTGA 7 cut(s) 117, 154, 202, 346, 503, 538, 593
Hpy166II GTNNAC 2 cut(s) 301, 736
Hpy188I TCNGA 4 cut(s) 24, 42, 523, 571
Hpy188III TCNNGA 1 cut(s) 948
Hpy8I GTNNAC 2 cut(s) 301, 736
Hpy99I CGWCG 1 cut(s) 229
HpyAV CCTTC 4 cut(s) 69, 142, 287, 513
HpyCH4III ACNGT 1 cut(s) 321
HpyCH4V TGCA 2 cut(s) 871, 886
HpyF10VI GCNNNNNNNGC 2 cut(s) 374, 818
HpyF3I CTNAG 4 cut(s) 41, 405, 471, 531
Hsp92II CATG 3 cut(s) 368, 517, 856
HspAI GCGC 1 cut(s) 535
KflI GGGWCCC 1 cut(s) 265
Ksp22I TGATCA 2 cut(s) 24, 583
Kzo9I GATC 4 cut(s) 24, 560, 583, 801
LmnI GCTCC 3 cut(s) 35, 95, 809
Lsp1109I GCAGC 1 cut(s) 20
LweI GCATC 1 cut(s) 506
MabI ACCWGGT 1 cut(s) 909
MaeI CTAG 1 cut(s) 794
MaeIII GTNAC 1 cut(s) 334
MalI GATC 4 cut(s) 26, 562, 585, 803
MboI GATC 4 cut(s) 24, 560, 583, 801
MboII GAAGA 9 cut(s) 54, 57, 60, 63, 66, 69, 266, 658, 691
MfeI CAATTG 1 cut(s) 326
MflI RGATCY 1 cut(s) 560
MhlI GDGCHC 3 cut(s) 47, 406, 823
MluCI AATT 4 cut(s) 172, 217, 326, 878
MlyI GAGTC 2 cut(s) 31, 697
MmeI TCCRAC 1 cut(s) 144
MseI TTAA 2 cut(s) 309, 849
MspA1I CMGCKG 2 cut(s) 409, 589
MspR9I CCNGG 6 cut(s) 34, 262, 675, 788, 840, 911
MunI CAATTG 1 cut(s) 326
MvaI CCWGG 6 cut(s) 34, 262, 675, 788, 840, 911
MwoI GCNNNNNNNGC 2 cut(s) 374, 818
NdeII GATC 4 cut(s) 24, 560, 583, 801
NlaIII CATG 3 cut(s) 368, 517, 856
NlaIV GGNNCC 3 cut(s) 266, 267, 785
NmuCI GTSAC 1 cut(s) 334
PaqCI CACCTGC 1 cut(s) 280
PasI CCCWGGG 1 cut(s) 674
PfeI GAWTC 1 cut(s) 695
PfoI TCCNGGA 2 cut(s) 32, 786
PkrI GCNGC 1 cut(s) 10
PleI GAGTC 2 cut(s) 31, 697
PpsI GAGTC 2 cut(s) 31, 697
PpuMI RGGWCCY 1 cut(s) 265
Psp5II RGGWCCY 1 cut(s) 265
Psp6I CCWGG 6 cut(s) 32, 260, 673, 786, 838, 909
PspGI CCWGG 6 cut(s) 32, 260, 673, 786, 838, 909
PspN4I GGNNCC 3 cut(s) 266, 267, 785
PspPI GGNCC 3 cut(s) 265, 437, 863
PspPPI RGGWCCY 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 538
PsuI RGATCY 1 cut(s) 560
PvuII CAGCTG 2 cut(s) 409, 589
RsaI GTAC 3 cut(s) 593, 616, 915
RsaNI GTAC 3 cut(s) 592, 615, 914
SaqAI TTAA 2 cut(s) 309, 849
SatI GCNGC 1 cut(s) 9
Sau3AI GATC 4 cut(s) 24, 560, 583, 801
Sau96I GGNCC 3 cut(s) 265, 437, 863
SchI GAGTC 2 cut(s) 31, 697
ScrFI CCNGG 6 cut(s) 34, 262, 675, 788, 840, 911
SduI GDGCHC 3 cut(s) 47, 406, 823
SexAI ACCWGGT 1 cut(s) 909
SfaNI GCATC 1 cut(s) 506
SfcI CTRYAG 1 cut(s) 900
SinI GGWCC 1 cut(s) 265
Sse9I AATT 4 cut(s) 172, 217, 326, 878
SsiI CCGC 1 cut(s) 213
SspMI CTAG 1 cut(s) 794
StyD4I CCNGG 6 cut(s) 32, 260, 673, 786, 838, 909
TaaI ACNGT 1 cut(s) 321
TaqI TCGA 4 cut(s) 227, 381, 393, 506
TasI AATT 4 cut(s) 172, 217, 326, 878
TatI WGTACW 1 cut(s) 591
TfiI GAWTC 1 cut(s) 695
Tru1I TTAA 2 cut(s) 309, 849
Tru9I TTAA 2 cut(s) 309, 849
TscAI CASTG 2 cut(s) 468, 865
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 1 cut(s) 8
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 2 cut(s) 17, 124
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 2 cut(s) 468, 865
VpaK11BI GGWCC 1 cut(s) 265
XspI CTAG 1 cut(s) 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.