RLG00000010396

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010419
Physical Location & Seq
Reverse (-)
1335549 .. 1339983
4435 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010396

Sequence Viewer

Length: 1149 bp
ATGAAAGCAGCACTATCCACGCTGATCAGCTCCAAGACTCTTAGCCCTCACCCCTCTTCTTCTTCTTCTTTCTCTCGCTCCTTTTGGCTTCAACCAAAGACCCTCTCCAACTCACCCTCCTTCATTACCCTTATCCCCTCCAAGACCCTCACCACCAACACCAACAATTACTCCCATCTCGGCCTCAATCATCGCAAGTCCCACACTGCTAATTTCTCGTCGATAATGGCCTCCGCTTATAAGCCCGAAGAAGCCAGGGTCCCACCTGCCCTTCCCTTGCCCACTCCTCCGGTCAACAAGTTTAAGATTGGACTGTGCCAATTGTCGGTGACGGCCGATAAGGAGAGGAACATTGTTCATGCTCGCAAGGCCATTGAGCAGGCTGCCGAAAAGGGTGCTCAGCTGGTTCTCCTACCTGAAATATGGAATGGCCCATATTCAAATGATAGCTTCCCAGTGTTTGCTGAGGATATTGACGCTGGTGGTAATGCATCTCCTTCAACAGCCATGCTTTCTGAAGTTGCTCAGCGCCTGAAAATCACCATTGTTGGTGGATCTATATCAGAGCGTTCTGGTGATCAGCTGTACAATACTTGTTGTGTCTTTGGTACTGATGGAAAGCTGAAAGCTAAACATAGGAAGATACACCTCTTTGATATTGATATCCCTGGGAAGATTACCTTTATTGAATCAAAGACTCTTACAGCAGGGCAAACTCCTACCATTGTGGACACAGAAGTTGGGCGTATTGGCATAGGCATTTGTTATGACATTAGGTTCCAGGAACTAGCTATGATCTATGGAGCAAGAGGTGCTCACTTGATATGCTATCCTGGGGCTTTTAACATGACCACTGGGCCATTGCATTGGGAATTGTTGCAGAGAGCAAGGGCTACAGATAACCAGCTATATGTGGCAACTTGTTCACCTGCTCGAGATGCCGGAGCTGGTTATGTGGCATGGGGCCACTCCACCCTTGTCGGACCATTTGGAGAAGTGCTGGCAACTACAGAACATGAAGAGGCAATAATCATAGCTGAGGTTGATTATTCACTCCTTGAGCTTAGAAGAACAAATCTACCTTTACTGAAGCAGCGGCGAGGTGATCTTTACCAGGTGGTAGATGTTCAGAGGTTGAATTCTAAATGA

Protein Analysis

383

Amino Acids

41.72

Weight (kDa)

8.78

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 102 - 354 2.7e-64 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 240
AarI CACCTGC 2 cut(s) 274, 937
Acc36I ACCTGC 2 cut(s) 274, 937
AciI CCGC 2 cut(s) 234, 1096
AclWI GGATC 1 cut(s) 562
AcoI YGGCCR 1 cut(s) 333
AcsI RAATTY 1 cut(s) 1138
AcuI CTGAAG 2 cut(s) 537, 1109
AfaI GTAC 2 cut(s) 587, 610
AfiI CCNNNNNNNGG 1 cut(s) 325
AgsI TTSAA 5 cut(s) 92, 441, 501, 689, 1138
AjnI CCWGG 5 cut(s) 254, 667, 780, 832, 1113
Alw21I GWGCWC 2 cut(s) 400, 817
AlwI GGATC 1 cut(s) 562
AlwNI CAGNNNCTG 1 cut(s) 532
Ama87I CYCGRG 1 cut(s) 933
AoxI GGCC 7 cut(s) 181, 228, 333, 369, 430, 857, 964
ApeKI GCWGC 3 cut(s) 8, 383, 1093
ApoI RAATTY 1 cut(s) 1138
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 5 cut(s) 259, 431, 857, 964, 983
AsuHPI GGTGA 8 cut(s) 41, 105, 142, 340, 532, 587, 918, 1115
AvaI CYCGRG 1 cut(s) 933
AvaII GGWCC 2 cut(s) 259, 983
Bbv12I GWGCWC 2 cut(s) 400, 817
BbvCI CCTCAGC 2 cut(s) 465, 1038
BbvI GCAGC 3 cut(s) 20, 370, 1105
BccI CCATC 2 cut(s) 183, 608
BceAI ACGGC 1 cut(s) 348
BcgI CGANNNNNNTGC 2 cut(s) 377, 411
BciT130I CCWGG 5 cut(s) 256, 669, 782, 834, 1115
BclI TGATCA 2 cut(s) 24, 577
BfaI CTAG 1 cut(s) 788
BfmI CTRYAG 2 cut(s) 894, 1008
BfoI RGCGCY 1 cut(s) 532
BfuAI ACCTGC 2 cut(s) 274, 937
BisI GCNGC 4 cut(s) 9, 384, 1094, 1097
BlpI GCTNAGC 2 cut(s) 399, 525
BlsI GCNGC 4 cut(s) 10, 385, 1095, 1098
Bme1390I CCNGG 5 cut(s) 256, 669, 782, 834, 1115
Bme18I GGWCC 2 cut(s) 259, 983
BmeT110I CYCGRG 1 cut(s) 933
BmgT120I GGNCC 5 cut(s) 259, 431, 857, 964, 983
BmiI GGNNCC 4 cut(s) 260, 261, 779, 965
BmrFI CCNGG 5 cut(s) 256, 669, 782, 834, 1115
BmrI ACTGGG 2 cut(s) 449, 864
BmsI GCATC 2 cut(s) 500, 928
BmuI ACTGGG 2 cut(s) 449, 864
Bpu10I CCTNAGC 2 cut(s) 465, 1038
Bpu1102I GCTNAGC 2 cut(s) 399, 525
BpuEI CTTGAG 1 cut(s) 1079
BsaBI GATNNNNATC 1 cut(s) 559
BsaJI CCNNGG 4 cut(s) 255, 667, 668, 833
BsaWI WCCGGW 1 cut(s) 289
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 155, 185
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse1I ACTGG 2 cut(s) 455, 859
Bse3DI GCAATG 1 cut(s) 860
Bse8I GATNNNNATC 1 cut(s) 559
BseBI CCWGG 5 cut(s) 256, 669, 782, 834, 1115
BseDI CCNNGG 4 cut(s) 255, 667, 668, 833
BseJI GATNNNNATC 1 cut(s) 559
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMI GCAATG 1 cut(s) 860
BseMII CTCAG 4 cut(s) 413, 456, 539, 1029
BseNI ACTGG 2 cut(s) 455, 859
BseRI GAGGAG 1 cut(s) 276
BseX3I CGGCCG 1 cut(s) 333
BseXI GCAGC 3 cut(s) 20, 370, 1105
Bsh1285I CGRYCG 1 cut(s) 336
BshFI GGCC 7 cut(s) 183, 230, 335, 371, 432, 859, 966
BsiEI CGRYCG 1 cut(s) 336
BsiHKAI GWGCWC 2 cut(s) 400, 817
BsiHKCI CYCGRG 1 cut(s) 933
BsiSI CCGG 2 cut(s) 290, 942
BslFI GGGAC 2 cut(s) 184, 245
BslI CCNNNNNNNGG 1 cut(s) 325
BsmFI GGGAC 2 cut(s) 184, 245
BsnI GGCC 7 cut(s) 183, 230, 335, 371, 432, 859, 966
BsoBI CYCGRG 1 cut(s) 933
Bsp1286I GDGCHC 2 cut(s) 400, 817
Bsp1407I TGTACA 1 cut(s) 585
Bsp143I GATC 5 cut(s) 24, 554, 577, 795, 1105
Bsp1720I GCTNAGC 2 cut(s) 399, 525
BspACI CCGC 2 cut(s) 234, 1096
BspANI GGCC 7 cut(s) 183, 230, 335, 371, 432, 859, 966
BspCNI CTCAG 4 cut(s) 412, 457, 538, 1030
BspLI GGNNCC 4 cut(s) 260, 261, 779, 965
BspMI ACCTGC 2 cut(s) 274, 937
BspPI GGATC 1 cut(s) 562
BsrDI GCAATG 1 cut(s) 860
BsrGI TGTACA 1 cut(s) 585
BsrI ACTGG 2 cut(s) 455, 859
BssECI CCNNGG 4 cut(s) 255, 667, 668, 833
BssMI GATC 5 cut(s) 24, 554, 577, 795, 1105
Bst2UI CCWGG 5 cut(s) 256, 669, 782, 834, 1115
Bst4CI ACNGT 1 cut(s) 315
Bst6I CTCTTC 2 cut(s) 61, 1014
BstAPI GCANNNNNTGC 1 cut(s) 812
BstAUI TGTACA 1 cut(s) 585
BstC8I GCNNGC 3 cut(s) 364, 381, 1002
BstDEI CTNAG 6 cut(s) 41, 399, 465, 525, 1038, 1064
BstH2I RGCGCY 1 cut(s) 532
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 5 cut(s) 27, 557, 580, 798, 1108
BstMBI GATC 5 cut(s) 24, 554, 577, 795, 1105
BstMCI CGRYCG 1 cut(s) 336
BstMWI GCNNNNNNNGC 3 cut(s) 368, 812, 938
BstNI CCWGG 5 cut(s) 256, 669, 782, 834, 1115
BstSCI CCNGG 5 cut(s) 254, 667, 780, 832, 1113
BstSFI CTRYAG 2 cut(s) 894, 1008
BstV1I GCAGC 3 cut(s) 20, 370, 1105
BstX2I RGATCY 1 cut(s) 554
BstXI CCANNNNNNTGG 1 cut(s) 867
BstYI RGATCY 1 cut(s) 554
BstZI CGGCCG 1 cut(s) 333
BsuRI GGCC 7 cut(s) 183, 230, 335, 371, 432, 859, 966
BtgZI GCGATG 1 cut(s) 176
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 3 cut(s) 204, 462, 852
BveI ACCTGC 2 cut(s) 274, 937
Cac8I GCNNGC 3 cut(s) 364, 381, 1002
CaiI CAGNNNCTG 1 cut(s) 532
CfoI GCGC 1 cut(s) 531
Cfr13I GGNCC 5 cut(s) 259, 431, 857, 964, 983
CseI GACGC 1 cut(s) 485
CsiI ACCWGGT 1 cut(s) 1113
Csp6I GTAC 2 cut(s) 586, 609
CviAII CATG 5 cut(s) 359, 508, 847, 960, 1016
CviQI GTAC 2 cut(s) 586, 609
DdeI CTNAG 6 cut(s) 41, 399, 465, 525, 1038, 1064
DpnI GATC 5 cut(s) 26, 556, 579, 797, 1107
DpnII GATC 5 cut(s) 24, 554, 577, 795, 1105
EaeI YGGCCR 1 cut(s) 333
EagI CGGCCG 1 cut(s) 333
Eam1104I CTCTTC 2 cut(s) 61, 1014
EarI CTCTTC 2 cut(s) 61, 1014
EclXI CGGCCG 1 cut(s) 333
Eco32I GATATC 1 cut(s) 664
Eco47I GGWCC 2 cut(s) 259, 983
Eco52I CGGCCG 1 cut(s) 333
Eco57I CTGAAG 2 cut(s) 537, 1109
Eco88I CYCGRG 1 cut(s) 933
EcoO109I RGGNCCY 1 cut(s) 259
EcoRI GAATTC 1 cut(s) 1138
EcoRII CCWGG 5 cut(s) 254, 667, 780, 832, 1113
EcoRV GATATC 1 cut(s) 664
EcoT22I ATGCAT 1 cut(s) 493
FaeI CATG 5 cut(s) 362, 511, 850, 963, 1019
FalI AAGNNNNNCTT 2 cut(s) 665, 697
FaqI GGGAC 2 cut(s) 184, 245
FatI CATG 5 cut(s) 358, 507, 846, 959, 1015
FbaI TGATCA 2 cut(s) 24, 577
Fnu4HI GCNGC 4 cut(s) 9, 384, 1094, 1097
Fsp4HI GCNGC 4 cut(s) 9, 384, 1094, 1097
FspBI CTAG 1 cut(s) 788
GlaI GCGC 1 cut(s) 530
GluI GCNGC 4 cut(s) 9, 384, 1094, 1097
HaeII RGCGCY 1 cut(s) 532
HaeIII GGCC 7 cut(s) 183, 230, 335, 371, 432, 859, 966
HapII CCGG 2 cut(s) 290, 942
HgaI GACGC 1 cut(s) 485
HhaI GCGC 1 cut(s) 531
Hin1II CATG 5 cut(s) 362, 511, 850, 963, 1019
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 3 cut(s) 37, 689, 697
HpaII CCGG 2 cut(s) 290, 942
HphI GGTGA 8 cut(s) 41, 105, 142, 340, 532, 587, 918, 1115
Hpy166II GTNNAC 3 cut(s) 295, 730, 926
Hpy188I TCNGA 4 cut(s) 517, 565, 983, 1131
Hpy188III TCNNGA 1 cut(s) 935
Hpy8I GTNNAC 3 cut(s) 295, 730, 926
Hpy99I CGWCG 1 cut(s) 223
HpyAV CCTTC 3 cut(s) 130, 281, 507
HpyCH4III ACNGT 1 cut(s) 315
HpyCH4V TGCA 3 cut(s) 491, 865, 880
HpyF10VI GCNNNNNNNGC 3 cut(s) 368, 812, 938
HpyF3I CTNAG 6 cut(s) 41, 399, 465, 525, 1038, 1064
Hsp92II CATG 5 cut(s) 362, 511, 850, 963, 1019
HspAI GCGC 1 cut(s) 529
KflI GGGWCCC 1 cut(s) 259
Ksp22I TGATCA 2 cut(s) 24, 577
Kzo9I GATC 5 cut(s) 24, 554, 577, 795, 1105
LmnI GCTCC 4 cut(s) 35, 83, 803, 944
Lsp1109I GCAGC 3 cut(s) 20, 370, 1105
LweI GCATC 2 cut(s) 500, 928
MabI ACCWGGT 1 cut(s) 1113
MaeI CTAG 1 cut(s) 788
MaeIII GTNAC 1 cut(s) 328
MalI GATC 5 cut(s) 26, 556, 579, 797, 1107
MboI GATC 5 cut(s) 24, 554, 577, 795, 1105
MboII GAAGA 9 cut(s) 48, 51, 54, 57, 260, 652, 685, 1031, 1080
MfeI CAATTG 1 cut(s) 320
MflI RGATCY 1 cut(s) 554
MhlI GDGCHC 2 cut(s) 400, 817
MluCI AATT 5 cut(s) 166, 211, 320, 872, 1138
MlyI GAGTC 2 cut(s) 31, 691
MmeI TCCRAC 2 cut(s) 132, 961
Mph1103I ATGCAT 1 cut(s) 493
MseI TTAA 2 cut(s) 303, 843
MspA1I CMGCKG 3 cut(s) 403, 583, 1096
MspI CCGG 2 cut(s) 290, 942
MspR9I CCNGG 5 cut(s) 256, 669, 782, 834, 1115
MunI CAATTG 1 cut(s) 320
MvaI CCWGG 5 cut(s) 256, 669, 782, 834, 1115
MwoI GCNNNNNNNGC 3 cut(s) 368, 812, 938
NdeII GATC 5 cut(s) 24, 554, 577, 795, 1105
NlaIII CATG 5 cut(s) 362, 511, 850, 963, 1019
NlaIV GGNNCC 4 cut(s) 260, 261, 779, 965
NmeAIII GCCGAG 1 cut(s) 159
NmuCI GTSAC 1 cut(s) 328
NsiI ATGCAT 1 cut(s) 493
PaeR7I CTCGAG 1 cut(s) 933
PaqCI CACCTGC 2 cut(s) 274, 937
PasI CCCWGGG 1 cut(s) 668
PfeI GAWTC 1 cut(s) 689
PfoI TCCNGGA 1 cut(s) 780
PkrI GCNGC 4 cut(s) 10, 385, 1095, 1098
PleI GAGTC 2 cut(s) 31, 691
PpsI GAGTC 2 cut(s) 31, 691
PpuMI RGGWCCY 1 cut(s) 259
PsiI TTATAA 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 5 cut(s) 254, 667, 780, 832, 1113
PspGI CCWGG 5 cut(s) 254, 667, 780, 832, 1113
PspN4I GGNNCC 4 cut(s) 260, 261, 779, 965
PspPI GGNCC 5 cut(s) 259, 431, 857, 964, 983
PspPPI RGGWCCY 1 cut(s) 259
PstNI CAGNNNCTG 1 cut(s) 532
PsuI RGATCY 1 cut(s) 554
PvuII CAGCTG 2 cut(s) 403, 583
RsaI GTAC 2 cut(s) 587, 610
RsaNI GTAC 2 cut(s) 586, 609
SaqAI TTAA 2 cut(s) 303, 843
SatI GCNGC 4 cut(s) 9, 384, 1094, 1097
Sau3AI GATC 5 cut(s) 24, 554, 577, 795, 1105
Sau96I GGNCC 5 cut(s) 259, 431, 857, 964, 983
SchI GAGTC 2 cut(s) 31, 691
ScrFI CCNGG 5 cut(s) 256, 669, 782, 834, 1115
SduI GDGCHC 2 cut(s) 400, 817
SexAI ACCWGGT 1 cut(s) 1113
SfaNI GCATC 2 cut(s) 500, 928
SfcI CTRYAG 2 cut(s) 894, 1008
Sfr274I CTCGAG 1 cut(s) 933
SinI GGWCC 2 cut(s) 259, 983
SlaI CTCGAG 1 cut(s) 933
SmlI CTYRAG 2 cut(s) 933, 1058
SmoI CTYRAG 2 cut(s) 933, 1058
Sse9I AATT 5 cut(s) 166, 211, 320, 872, 1138
SsiI CCGC 2 cut(s) 234, 1096
SspMI CTAG 1 cut(s) 788
StyD4I CCNGG 5 cut(s) 254, 667, 780, 832, 1113
TaaI ACNGT 1 cut(s) 315
TaqI TCGA 2 cut(s) 221, 934
TasI AATT 5 cut(s) 166, 211, 320, 872, 1138
TatI WGTACW 1 cut(s) 585
TauI GCSGC 1 cut(s) 1099
TfiI GAWTC 1 cut(s) 689
Tru1I TTAA 2 cut(s) 303, 843
Tru9I TTAA 2 cut(s) 303, 843
TscAI CASTG 3 cut(s) 211, 462, 859
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 3 cut(s) 8, 383, 1093
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 4 cut(s) 17, 112, 347, 1032
TspRI CASTG 3 cut(s) 211, 462, 859
VpaK11BI GGWCC 2 cut(s) 259, 983
XapI RAATTY 1 cut(s) 1138
XhoI CTCGAG 1 cut(s) 933
XspI CTAG 1 cut(s) 788
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.