AT5G15110

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003744
Physical Location & Seq
Forward (+)
4895750 .. 4897966
2217 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G15110.1

Sequence Viewer

Length: 1419 bp
ATGGAGATGGTTAGGCTGTCAAAGTTGATGTTTACGTTTTGTATTGCCGTTCTAATTCCGACCATTCGAGGTAACATTTCTGAGCTGGACGAGTATTGGTCGCAACGAGCCGACGAAGCCAGAGAATTCACTCTTCAAGCTTACCATTCTGATCCTTACGAAATCGTCGATCACTTTCACGAGCGTCACTACGACAACTCTACTGATGTTACAACGCCTGAGGAAGATGGTGATGCGAAGCCCGAAGAGGAGGAAAAAGAGTTCATTGAAATGCTCGGAAGCTCGACGAACAGCACCAGACGAAGCCTAAGAGGTAAAGGCAAGGGAAAATGGAGTAAGCTAAAGGGACCTTGTACCGCAAGTAACCCCATAGATAAATGTTGGCGTTGCCGATCGGATTGGGCTAAGCGTCGGAAGAAGCTTACACGTTGTGTCCGAGGGTTTGGTCACAGGACGACCGGAGGAAAACGAGGACGCATCTATGTGGTTACAAGCAACCTCGACGAAGACATGGTGAACCCTAAACCGGGGACATTGCGTCATGCCGTGATTCAGAAGGAACCTCTGTGGATCATTTTCAAGAACGATATGAGCATTCGGTTAAACCAAGAGCTTCTGATTAATAGCCACAAGACTATCGATGCACGAGGAGCTAATGTCCATGTAGCCCATGGCGCAGGGATCACAATGCAGTTTGTGAAAAATGTCGTCATCCATGGACTGCATATTCACCACATTTCTGAGAGTAGCGGTGGTATGATCCGAGACTCTGTAGACCATTTCGGAATGAGGACTAGAGCTGATGGAGATGGTCTATCGATTTATGGTTCGTCTAACATTTGGCTTGACCATATCTCGATGTCGAAATGTCAAGATGGACTCATCGATGCCATTGTTGGATCCACTGGCATTACAATCTCCAACTCCCACTTCACTCACCACAACGATGTGATGTTGCTTGGTGCTCAAAACACGAATGAAGCGGACAAACATATGCAAGTCACAGTGGCTTATAACCATTTTGGTAAAGGACTTGTACAGAGAATGCCTAGGATCCGATGGGGATTTGTTCATGTTGTGAACAACGACTACACTCATTGGGAGCTTTACGCGATCGGAGGTAGTCAAGGTCCTACGATTCTCAGCCATGGAAACCGATTCATAGCTCCTCCACACAAACCGCATTACAGAGAGGTGACAAAGAGAGATTATGCTTCGGAGGACGAGTGGAAACACTGGAACTGGAGATCTGATAAAGATGTATTCATGAACGGTGCTTATTTCAGACAATCTGGTAATCCTCAATACAAATGTGCACACACAAGACAACAAATGATCAAACCGAAAAATGGATTGGCTGTTTCAAAGCTGACCAAATATGCCGGAGCACTTGATTGCAGAGTCGGGAGACGCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C Pec_lyase_N

Protein Analysis

472

Amino Acids

53.84

Weight (kDa)

9.17

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pec_lyase_N PF04431 25 - 103 8.7e-20 Pectate lyase, N terminus
Pectate_lyase_4 PF00544 204 - 388 1.7e-17 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1014
AccI GTMKAC 1 cut(s) 774
AccII CGCG 1 cut(s) 1112
AciI CCGC 4 cut(s) 357, 750, 983, 1181
AclWI GGATC 8 cut(s) 146, 578, 689, 754, 894, 907, 1048, 1061
AcsI RAATTY 1 cut(s) 125
AdeI CACNNNGTG 1 cut(s) 431
AfaI GTAC 2 cut(s) 355, 1038
AfiI CCNNNNNNNGG 3 cut(s) 526, 527, 1349
AflIII ACRYGT 1 cut(s) 425
AgsI TTSAA 4 cut(s) 137, 269, 580, 1365
AhdI GACNNNNNGTC 1 cut(s) 537
AjuI GAANNNNNNNTTGG 4 cut(s) 914, 946, 1337, 1369
AloI GAACNNNNNNTCC 2 cut(s) 245, 277
Alw21I GWGCWC 3 cut(s) 967, 1318, 1390
Alw26I GTCTC 2 cut(s) 759, 1402
Alw44I GTGCAC 1 cut(s) 1314
AlwI GGATC 8 cut(s) 146, 578, 689, 754, 894, 907, 1048, 1061
ApaLI GTGCAC 1 cut(s) 1314
ApoI RAATTY 1 cut(s) 125
AseI ATTAAT 1 cut(s) 621
AspA2I CCTAGG 1 cut(s) 1049
AspLEI GCGC 1 cut(s) 677
AspS9I GGNCC 2 cut(s) 347, 1130
AsuC2I CCSGG 1 cut(s) 528
AsuHPI GGTGA 5 cut(s) 242, 526, 722, 929, 1207
AvaII GGWCC 2 cut(s) 347, 1130
AvrII CCTAGG 1 cut(s) 1049
AxyI CCTNAGG 1 cut(s) 219
BaeGI GKGCMC 1 cut(s) 1318
BamHI GGATCC 2 cut(s) 899, 1053
BauI CACGAG 2 cut(s) 179, 645
BbsI GAAGAC 1 cut(s) 513
Bbv12I GWGCWC 3 cut(s) 967, 1318, 1390
BccI CCATC 5 cut(s) 221, 797, 803, 869, 1053
BceAI ACGGC 2 cut(s) 32, 530
BclI TGATCA 1 cut(s) 1335
BcnI CCSGG 1 cut(s) 528
BcoDI GTCTC 2 cut(s) 759, 1402
BfaI CTAG 2 cut(s) 795, 1050
BfmI CTRYAG 1 cut(s) 771
BglII AGATCT 1 cut(s) 1247
BlnI CCTAGG 1 cut(s) 1049
BlpI GCTNAGC 1 cut(s) 405
Bme1390I CCNGG 1 cut(s) 528
Bme18I GGWCC 2 cut(s) 347, 1130
BmeRI GACNNNNNGTC 1 cut(s) 537
BmgT120I GGNCC 2 cut(s) 347, 1130
BmiI GGNNCC 4 cut(s) 348, 561, 901, 1055
BmrFI CCNGG 1 cut(s) 528
BmsI GCATC 4 cut(s) 223, 486, 631, 877
BpiI GAAGAC 1 cut(s) 513
BpmI CTGGAG 1 cut(s) 1264
Bpu1102I GCTNAGC 1 cut(s) 405
BpuMI CCSGG 1 cut(s) 528
Bsa29I ATCGAT 3 cut(s) 639, 818, 885
BsaJI CCNNGG 6 cut(s) 436, 527, 670, 715, 1049, 1147
BsaWI WCCGGW 1 cut(s) 458
BsaXI ACNNNNNCTCC 5 cut(s) 642, 672, 1211, 1241, 1399
Bsc4I CCNNNNNNNGG 3 cut(s) 526, 527, 1349
Bse1I ACTGG 3 cut(s) 910, 1241, 1247
Bse21I CCTNAGG 1 cut(s) 219
Bse3DI GCAATG 1 cut(s) 533
BseCI ATCGAT 3 cut(s) 639, 818, 885
BseDI CCNNGG 6 cut(s) 436, 527, 670, 715, 1049, 1147
BseGI GGATG 1 cut(s) 711
BseLI CCNNNNNNNGG 3 cut(s) 526, 527, 1349
BseMI GCAATG 1 cut(s) 533
BseMII CTCAG 4 cut(s) 72, 210, 732, 1156
BseNI ACTGG 3 cut(s) 910, 1241, 1247
BseRI GAGGAG 3 cut(s) 263, 663, 1158
BseSI GKGCMC 1 cut(s) 1318
Bsh1236I CGCG 1 cut(s) 1112
Bsh1285I CGRYCG 3 cut(s) 395, 459, 1116
BshVI ATCGAT 3 cut(s) 639, 818, 885
BsiEI CGRYCG 3 cut(s) 395, 459, 1116
BsiHKAI GWGCWC 3 cut(s) 967, 1318, 1390
BsiSI CCGG 3 cut(s) 459, 527, 1383
BslFI GGGAC 2 cut(s) 360, 544
BslI CCNNNNNNNGG 3 cut(s) 526, 527, 1349
BsmAI GTCTC 2 cut(s) 759, 1402
BsmBI CGTCTC 1 cut(s) 1402
BsmFI GGGAC 2 cut(s) 360, 544
BsmI GAATGC 2 cut(s) 594, 1050
Bsp1286I GDGCHC 3 cut(s) 967, 1318, 1390
Bsp1407I TGTACA 1 cut(s) 1036
Bsp1720I GCTNAGC 1 cut(s) 405
Bsp19I CCATGG 3 cut(s) 670, 715, 1147
BspACI CCGC 4 cut(s) 357, 750, 983, 1181
BspCNI CTCAG 4 cut(s) 73, 211, 733, 1155
BspDI ATCGAT 3 cut(s) 639, 818, 885
BspFNI CGCG 1 cut(s) 1112
BspHI TCATGA 1 cut(s) 1266
BspLI GGNNCC 4 cut(s) 348, 561, 901, 1055
BspPI GGATC 8 cut(s) 146, 578, 689, 754, 894, 907, 1048, 1061
BsrDI GCAATG 1 cut(s) 533
BsrGI TGTACA 1 cut(s) 1036
BsrI ACTGG 3 cut(s) 910, 1241, 1247
BssECI CCNNGG 6 cut(s) 436, 527, 670, 715, 1049, 1147
BssSI CACGAG 2 cut(s) 179, 645
BssT1I CCWWGG 4 cut(s) 670, 715, 1049, 1147
Bst2BI CACGAG 2 cut(s) 179, 645
Bst4CI ACNGT 2 cut(s) 1006, 1274
Bst6I CTCTTC 2 cut(s) 138, 240
BstAUI TGTACA 1 cut(s) 1036
BstDEI CTNAG 6 cut(s) 81, 219, 308, 405, 741, 1142
BstDSI CCRYGG 3 cut(s) 670, 715, 1147
BstF5I GGATG 1 cut(s) 711
BstFNI CGCG 1 cut(s) 1112
BstHHI GCGC 1 cut(s) 677
BstMAI GTCTC 2 cut(s) 759, 1402
BstMCI CGRYCG 3 cut(s) 395, 459, 1116
BstMWI GCNNNNNNNGC 3 cut(s) 116, 650, 674
BstSCI CCNGG 1 cut(s) 526
BstSFI CTRYAG 1 cut(s) 771
BstSLI GKGCMC 1 cut(s) 1318
BstUI CGCG 1 cut(s) 1112
BstV2I GAAGAC 1 cut(s) 513
BstX2I RGATCY 3 cut(s) 899, 1053, 1247
BstYI RGATCY 3 cut(s) 899, 1053, 1247
Bsu15I ATCGAT 3 cut(s) 639, 818, 885
Bsu36I CCTNAGG 1 cut(s) 219
BsuTUI ATCGAT 3 cut(s) 639, 818, 885
BtgI CCRYGG 3 cut(s) 670, 715, 1147
BtsCI GGATG 1 cut(s) 711
BtsIMutI CAGTG 3 cut(s) 903, 1011, 1234
CciI TCATGA 1 cut(s) 1266
CfoI GCGC 1 cut(s) 677
Cfr13I GGNCC 2 cut(s) 347, 1130
ClaI ATCGAT 3 cut(s) 639, 818, 885
CseI GACGC 4 cut(s) 173, 398, 483, 527
Csp6I GTAC 2 cut(s) 354, 1037
CviAII CATG 8 cut(s) 511, 542, 662, 671, 716, 1073, 1148, 1267
CviQI GTAC 2 cut(s) 354, 1037
DdeI CTNAG 6 cut(s) 81, 219, 308, 405, 741, 1142
DraIII CACNNNGTG 1 cut(s) 431
DriI GACNNNNNGTC 1 cut(s) 537
Eam1104I CTCTTC 2 cut(s) 138, 240
Eam1105I GACNNNNNGTC 1 cut(s) 537
EarI CTCTTC 2 cut(s) 138, 240
Eco130I CCWWGG 4 cut(s) 670, 715, 1049, 1147
Eco47I GGWCC 2 cut(s) 347, 1130
Eco81I CCTNAGG 1 cut(s) 219
EcoO109I RGGNCCY 2 cut(s) 347, 1130
EcoRI GAATTC 1 cut(s) 125
EcoT14I CCWWGG 4 cut(s) 670, 715, 1049, 1147
ErhI CCWWGG 4 cut(s) 670, 715, 1049, 1147
Esp3I CGTCTC 1 cut(s) 1402
FaeI CATG 8 cut(s) 514, 545, 665, 674, 719, 1076, 1151, 1270
FaqI GGGAC 2 cut(s) 360, 544
FatI CATG 8 cut(s) 510, 541, 661, 670, 715, 1072, 1147, 1266
FauNDI CATATG 1 cut(s) 993
FbaI TGATCA 1 cut(s) 1335
FblI GTMKAC 1 cut(s) 774
FokI GGATG 1 cut(s) 698
FspBI CTAG 2 cut(s) 795, 1050
GlaI GCGC 1 cut(s) 676
GsuI CTGGAG 1 cut(s) 1264
HapII CCGG 3 cut(s) 459, 527, 1383
HgaI GACGC 4 cut(s) 173, 398, 483, 527
HhaI GCGC 1 cut(s) 677
Hin1II CATG 8 cut(s) 514, 545, 665, 674, 719, 1076, 1151, 1270
Hin6I GCGC 1 cut(s) 675
HinP1I GCGC 1 cut(s) 675
HindIII AAGCTT 2 cut(s) 138, 419
HinfI GANTC 6 cut(s) 550, 767, 879, 1138, 1158, 1401
HpaII CCGG 3 cut(s) 459, 527, 1383
HphI GGTGA 5 cut(s) 242, 526, 722, 929, 1207
Hpy166II GTNNAC 5 cut(s) 33, 517, 775, 1081, 1316
Hpy188III TCNNGA 6 cut(s) 179, 580, 856, 872, 1267, 1405
Hpy8I GTNNAC 5 cut(s) 33, 517, 775, 1081, 1316
Hpy99I CGWCG 5 cut(s) 116, 170, 289, 414, 506
HpyAV CCTTC 1 cut(s) 550
HpyCH4III ACNGT 2 cut(s) 1006, 1274
HpyCH4IV ACGT 2 cut(s) 35, 427
HpyCH4V TGCA 6 cut(s) 644, 691, 724, 997, 1316, 1398
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 650, 674
HpyF3I CTNAG 6 cut(s) 81, 219, 308, 405, 741, 1142
HpySE526I ACGT 2 cut(s) 35, 427
Hsp92II CATG 8 cut(s) 514, 545, 665, 674, 719, 1076, 1151, 1270
HspAI GCGC 1 cut(s) 675
Ksp22I TGATCA 1 cut(s) 1335
LmnI GCTCC 4 cut(s) 650, 1102, 1171, 1385
LweI GCATC 4 cut(s) 223, 486, 631, 877
MaeI CTAG 2 cut(s) 795, 1050
MaeII ACGT 2 cut(s) 35, 427
MaeIII GTNAC 8 cut(s) 71, 185, 208, 362, 446, 487, 1000, 1195
MboII GAAGA 5 cut(s) 125, 236, 257, 427, 518
MflI RGATCY 3 cut(s) 899, 1053, 1247
MhlI GDGCHC 3 cut(s) 967, 1318, 1390
MluCI AATT 2 cut(s) 54, 125
MlyI GAGTC 3 cut(s) 761, 873, 1410
MmeI TCCRAC 4 cut(s) 83, 392, 877, 945
MseI TTAA 2 cut(s) 602, 621
MslI CAYNNNNRTG 3 cut(s) 269, 482, 945
MspI CCGG 3 cut(s) 459, 527, 1383
MspR9I CCNGG 1 cut(s) 528
Mva1269I GAATGC 2 cut(s) 594, 1050
MvnI CGCG 1 cut(s) 1112
MwoI GCNNNNNNNGC 3 cut(s) 116, 650, 674
NciI CCSGG 1 cut(s) 528
NcoI CCATGG 3 cut(s) 670, 715, 1147
NdeI CATATG 1 cut(s) 993
NlaIII CATG 8 cut(s) 514, 545, 665, 674, 719, 1076, 1151, 1270
NlaIV GGNNCC 4 cut(s) 348, 561, 901, 1055
NmuCI GTSAC 4 cut(s) 185, 446, 1000, 1195
PagI TCATGA 1 cut(s) 1266
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 433
PctI GAATGC 2 cut(s) 594, 1050
PfeI GAWTC 3 cut(s) 550, 1138, 1158
Ple19I CGATCG 2 cut(s) 395, 1116
PleI GAGTC 3 cut(s) 761, 873, 1409
PpsI GAGTC 3 cut(s) 761, 873, 1409
PpuMI RGGWCCY 2 cut(s) 347, 1130
PshBI ATTAAT 1 cut(s) 621
PsiI TTATAA 1 cut(s) 1014
Psp5II RGGWCCY 2 cut(s) 347, 1130
PspN4I GGNNCC 4 cut(s) 348, 561, 901, 1055
PspPI GGNCC 2 cut(s) 347, 1130
PspPPI RGGWCCY 2 cut(s) 347, 1130
PsrI GAACNNNNNNTAC 2 cut(s) 1073, 1105
PsuI RGATCY 3 cut(s) 899, 1053, 1247
PvuI CGATCG 2 cut(s) 395, 1116
RsaI GTAC 2 cut(s) 355, 1038
RsaNI GTAC 2 cut(s) 354, 1037
RseI CAYNNNNRTG 3 cut(s) 269, 482, 945
SaqAI TTAA 2 cut(s) 602, 621
Sau96I GGNCC 2 cut(s) 347, 1130
SchI GAGTC 3 cut(s) 761, 873, 1410
ScrFI CCNGG 1 cut(s) 528
SduI GDGCHC 3 cut(s) 967, 1318, 1390
SfaNI GCATC 4 cut(s) 223, 486, 631, 877
SfcI CTRYAG 1 cut(s) 771
SinI GGWCC 2 cut(s) 347, 1130
SmiMI CAYNNNNRTG 3 cut(s) 269, 482, 945
Sse9I AATT 2 cut(s) 54, 125
SsiI CCGC 4 cut(s) 357, 750, 983, 1181
SspMI CTAG 2 cut(s) 795, 1050
StyD4I CCNGG 1 cut(s) 526
StyI CCWWGG 4 cut(s) 670, 715, 1049, 1147
TaaI ACNGT 2 cut(s) 1006, 1274
TaiI ACGT 2 cut(s) 38, 430
TaqI TCGA 9 cut(s) 67, 168, 284, 501, 639, 818, 857, 863, 885
TasI AATT 2 cut(s) 54, 125
TatI WGTACW 1 cut(s) 1036
TfiI GAWTC 3 cut(s) 550, 1138, 1158
Tru1I TTAA 2 cut(s) 602, 621
Tru9I TTAA 2 cut(s) 602, 621
TscAI CASTG 3 cut(s) 910, 1011, 1241
TseFI GTSAC 4 cut(s) 185, 446, 1000, 1195
Tsp45I GTSAC 4 cut(s) 185, 446, 1000, 1195
TspDTI ATGAA 6 cut(s) 253, 993, 1061, 1150, 1255, 1283
TspRI CASTG 3 cut(s) 910, 1011, 1241
VneI GTGCAC 1 cut(s) 1314
VpaK11BI GGWCC 2 cut(s) 347, 1130
VspI ATTAAT 1 cut(s) 621
XapI RAATTY 1 cut(s) 125
XcmI CCANNNNNNNNNTGG 1 cut(s) 668
XmaJI CCTAGG 1 cut(s) 1049
XmiI GTMKAC 1 cut(s) 774
XspI CTAG 2 cut(s) 795, 1050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.