Rorug02G0536800

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003744
Physical Location & Seq
Forward (+)
66655581 .. 66656006
426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0536800.1

Sequence Viewer

Length: 426 bp
ATGAAAGGTAAGGGAGGACCTGAGAATTCTCGATGTAACTACAGAGGTGTTAGGCAGAGGACATGGGGCAAGTGGGTTGCTGAAATCCGCTCACCAAATAGGGGAAGTAGGCTCTGGCTAGGTACCTTTCCAACTGCAATTGAAGCTGCTCTTGCCTACGATGAAGCTGCAAGGGCCATGTACGGTTCCTCTGCCCGTCTCAACTTTCCGAAGGTTACGATCTCCAGTTACTCAACAAATTCTTCTTGTAGAGCAACTCTATCGGCTCCTTCCTGTGTAGTGACTTCTGCAGGTTCAGAATCATCAGGTCACTCTGAGGATTTTTCGCCTCAGGATAAGGTGAATTCTCATTCCCCTCACGTAGTGAACACTGATGTCATCGATCTAAATCAAATGGATCTAAATCATACTGAGGAGGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C Pec_lyase_N

Protein Analysis

141

Amino Acids

15.4

Weight (kDa)

6.42

Isoelectric Point (pI)

52.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 13 - 62 9.6e-16 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 281
Acc65I GGTACC 1 cut(s) 122
AccB1I GGYRCC 1 cut(s) 122
AccBSI CCGCTC 1 cut(s) 90
AciI CCGC 1 cut(s) 88
AclWI GGATC 1 cut(s) 405
AcsI RAATTY 3 cut(s) 25, 238, 343
AdeI CACNNNGTG 1 cut(s) 364
AfaI GTAC 2 cut(s) 124, 182
AfiI CCNNNNNNNGG 1 cut(s) 101
AgsI TTSAA 1 cut(s) 143
AluBI AGCT 2 cut(s) 146, 167
AluI AGCT 2 cut(s) 146, 167
Alw26I GTCTC 1 cut(s) 203
AlwI GGATC 1 cut(s) 405
AoxI GGCC 1 cut(s) 174
ApeKI GCWGC 2 cut(s) 146, 167
ApoI RAATTY 3 cut(s) 25, 238, 343
Asp718I GGTACC 1 cut(s) 122
AspS9I GGNCC 2 cut(s) 17, 174
AsuHPI GGTGA 2 cut(s) 84, 352
AvaII GGWCC 1 cut(s) 17
AxyI CCTNAGG 1 cut(s) 330
BanI GGYRCC 1 cut(s) 122
BbvI GCAGC 2 cut(s) 133, 154
BcgI CGANNNNNNTGC 4 cut(s) 149, 183, 243, 277
BcoDI GTCTC 1 cut(s) 203
BfaI CTAG 1 cut(s) 119
BfmI CTRYAG 2 cut(s) 40, 288
BfuAI ACCTGC 1 cut(s) 281
BisI GCNGC 2 cut(s) 147, 168
BlsI GCNGC 2 cut(s) 148, 169
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 2 cut(s) 17, 174
BmiI GGNNCC 3 cut(s) 124, 187, 267
BpmI CTGGAG 1 cut(s) 208
Bsa29I ATCGAT 1 cut(s) 381
BsaAI YACGTR 1 cut(s) 361
BsaBI GATNNNNATC 2 cut(s) 387, 402
Bsc4I CCNNNNNNNGG 1 cut(s) 101
Bse1I ACTGG 1 cut(s) 225
Bse21I CCTNAGG 1 cut(s) 330
Bse8I GATNNNNATC 2 cut(s) 387, 402
BseCI ATCGAT 1 cut(s) 381
BseGI GGATG 1 cut(s) 424
BseJI GATNNNNATC 2 cut(s) 387, 402
BseLI CCNNNNNNNGG 1 cut(s) 101
BseMII CTCAG 4 cut(s) 12, 306, 344, 402
BseNI ACTGG 1 cut(s) 225
BseXI GCAGC 2 cut(s) 133, 154
BshFI GGCC 1 cut(s) 176
BshNI GGYRCC 1 cut(s) 122
BshVI ATCGAT 1 cut(s) 381
BslI CCNNNNNNNGG 1 cut(s) 101
BsmAI GTCTC 1 cut(s) 203
BsmBI CGTCTC 1 cut(s) 203
BsnI GGCC 1 cut(s) 176
Bsp143I GATC 3 cut(s) 219, 382, 397
BspACI CCGC 1 cut(s) 88
BspANI GGCC 1 cut(s) 176
BspCNI CTCAG 4 cut(s) 13, 307, 343, 403
BspDI ATCGAT 1 cut(s) 381
BspLI GGNNCC 3 cut(s) 124, 187, 267
BspMAI CTGCAG 1 cut(s) 292
BspMI ACCTGC 1 cut(s) 281
BspPI GGATC 1 cut(s) 405
BspT107I GGYRCC 1 cut(s) 122
BsrBI CCGCTC 1 cut(s) 90
BsrI ACTGG 1 cut(s) 225
BssMI GATC 3 cut(s) 219, 382, 397
Bst4CI ACNGT 1 cut(s) 185
BstBAI YACGTR 1 cut(s) 361
BstDEI CTNAG 4 cut(s) 21, 315, 330, 411
BstF5I GGATG 1 cut(s) 424
BstKTI GATC 3 cut(s) 222, 385, 400
BstMAI GTCTC 1 cut(s) 203
BstMBI GATC 3 cut(s) 219, 382, 397
BstMWI GCNNNNNNNGC 3 cut(s) 143, 152, 173
BstSFI CTRYAG 2 cut(s) 40, 288
BstV1I GCAGC 2 cut(s) 133, 154
BstX2I RGATCY 1 cut(s) 397
BstYI RGATCY 1 cut(s) 397
Bsu15I ATCGAT 1 cut(s) 381
Bsu36I CCTNAGG 1 cut(s) 330
BsuRI GGCC 1 cut(s) 176
BsuTUI ATCGAT 1 cut(s) 381
BtsCI GGATG 1 cut(s) 424
BtsIMutI CAGTG 1 cut(s) 369
BveI ACCTGC 1 cut(s) 281
Cfr13I GGNCC 2 cut(s) 17, 174
ClaI ATCGAT 1 cut(s) 381
Csp6I GTAC 2 cut(s) 123, 181
CviAII CATG 2 cut(s) 63, 178
CviJI RGCY 6 cut(s) 112, 118, 146, 167, 176, 266
CviKI_1 RGCY 6 cut(s) 112, 118, 146, 167, 176, 266
CviQI GTAC 2 cut(s) 123, 181
DdeI CTNAG 4 cut(s) 21, 315, 330, 411
DpnI GATC 3 cut(s) 221, 384, 399
DpnII GATC 3 cut(s) 219, 382, 397
DraIII CACNNNGTG 1 cut(s) 364
Eco47I GGWCC 1 cut(s) 17
Eco81I CCTNAGG 1 cut(s) 330
EcoO109I RGGNCCY 1 cut(s) 17
EcoRI GAATTC 2 cut(s) 25, 343
Esp3I CGTCTC 1 cut(s) 203
FaeI CATG 2 cut(s) 66, 181
FaiI YATR 3 cut(s) 64, 179, 408
FalI AAGNNNNNCTT 2 cut(s) 135, 167
FatI CATG 2 cut(s) 62, 177
Fnu4HI GCNGC 2 cut(s) 147, 168
Fsp4HI GCNGC 2 cut(s) 147, 168
FspBI CTAG 1 cut(s) 119
GluI GCNGC 2 cut(s) 147, 168
GsuI CTGGAG 1 cut(s) 208
HaeIII GGCC 1 cut(s) 176
Hin1II CATG 2 cut(s) 66, 181
HinfI GANTC 1 cut(s) 299
HphI GGTGA 2 cut(s) 84, 352
Hpy166II GTNNAC 1 cut(s) 367
Hpy188I TCNGA 3 cut(s) 210, 298, 316
Hpy188III TCNNGA 2 cut(s) 30, 332
Hpy8I GTNNAC 1 cut(s) 367
HpyAV CCTTC 2 cut(s) 205, 279
HpyCH4III ACNGT 1 cut(s) 185
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 3 cut(s) 137, 170, 290
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 152, 173
HpyF3I CTNAG 4 cut(s) 21, 315, 330, 411
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 2 cut(s) 66, 181
KpnI GGTACC 1 cut(s) 126
Kzo9I GATC 3 cut(s) 219, 382, 397
LmnI GCTCC 1 cut(s) 271
LpnPI CCDG 7 cut(s) 33, 100, 238, 276, 286, 291, 317
Lsp1109I GCAGC 2 cut(s) 133, 154
MaeI CTAG 1 cut(s) 119
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 5 cut(s) 35, 214, 227, 280, 308
MalI GATC 3 cut(s) 221, 384, 399
MbiI CCGCTC 1 cut(s) 90
MboI GATC 3 cut(s) 219, 382, 397
MboII GAAGA 1 cut(s) 234
MfeI CAATTG 1 cut(s) 138
MflI RGATCY 1 cut(s) 397
MluCI AATT 4 cut(s) 25, 138, 238, 343
MmeI TCCRAC 1 cut(s) 155
MnlI CCTC 9 cut(s) 8, 38, 51, 199, 310, 339, 366, 406, 409
MunI CAATTG 1 cut(s) 138
MwoI GCNNNNNNNGC 3 cut(s) 143, 152, 173
NdeII GATC 3 cut(s) 219, 382, 397
NlaIII CATG 2 cut(s) 66, 181
NlaIV GGNNCC 3 cut(s) 124, 187, 267
NmuCI GTSAC 2 cut(s) 280, 308
PfeI GAWTC 1 cut(s) 299
PkrI GCNGC 2 cut(s) 148, 169
Ppu21I YACGTR 1 cut(s) 361
PpuMI RGGWCCY 1 cut(s) 17
Psp5II RGGWCCY 1 cut(s) 17
PspN4I GGNNCC 3 cut(s) 124, 187, 267
PspPI GGNCC 2 cut(s) 17, 174
PspPPI RGGWCCY 1 cut(s) 17
PstI CTGCAG 1 cut(s) 292
PsuI RGATCY 1 cut(s) 397
RsaI GTAC 2 cut(s) 124, 182
RsaNI GTAC 2 cut(s) 123, 181
SatI GCNGC 2 cut(s) 147, 168
Sau3AI GATC 3 cut(s) 219, 382, 397
Sau96I GGNCC 2 cut(s) 17, 174
SfcI CTRYAG 2 cut(s) 40, 288
SinI GGWCC 1 cut(s) 17
Sse9I AATT 4 cut(s) 25, 138, 238, 343
SsiI CCGC 1 cut(s) 88
SspMI CTAG 1 cut(s) 119
TaaI ACNGT 1 cut(s) 185
TaiI ACGT 1 cut(s) 363
TaqI TCGA 2 cut(s) 31, 381
TasI AATT 4 cut(s) 25, 138, 238, 343
TfiI GAWTC 1 cut(s) 299
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 2 cut(s) 280, 308
TseI GCWGC 2 cut(s) 146, 167
Tsp45I GTSAC 2 cut(s) 280, 308
TspDTI ATGAA 2 cut(s) 17, 177
TspRI CASTG 1 cut(s) 376
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 3 cut(s) 25, 238, 343
XspI CTAG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.