pycom06g16740

Pectate lyase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0003744
Physical Location & Seq
Forward (+)
20908078 .. 20908844
767 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g16740.1

Sequence Viewer

Length: 591 bp
ATGTATGGAGCCTCAAACATTTGGATAGACCATGTCTCCGCGTCAAATTGCGAGGATGGACTCATTGATGCCATCCAGGGGTCTACAGCCATCACCATATCCAATTGTCACTTCACCAATCATAATGATGTGATGTTATTTGGTTCAAGTGATAGCAACTCTCAGGATGAAGTGATGCAGATAACTCTTGCTTTCAACCATTTCGGTCAGGGGTTAACTCAGCGTATGCCAAGGTGCCGGTGGGGGTTTTTCCATGTTGTAAACAACGACTACACGCATTGGCTTATGTACGCTATTGGTGGCAGCCAACATCCTACCATAATTAGCCAGGGCAACCGCTTCATTGCTCCGTTAAATGAAGCGAGTAAAGAGGTGACTAAGAGGGAATATGCAGCAGAGAACGTGTGGAAATCGTGGAATTGGAGATCGGAGAACGATCTTATGATGAATGGAGCATTCTTTGTTGAATCTGGTAGCCCAATCAGGAATCTCCCAGAGGCGGATATGATCCAGGCAAAGCCTGGGACATTCGTGGCGAAGCTTACACGCTTTGCAGGTCCTCTTAAATGCATTAAAGGGAAGCCATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C Pec_lyase_N

Protein Analysis

197

Amino Acids

21.97

Weight (kDa)

6.36

Isoelectric Point (pI)

33.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 6 - 128 9.2e-21 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 545
AccB1I GGYRCC 1 cut(s) 234
AccI GTMKAC 1 cut(s) 83
AccII CGCG 1 cut(s) 41
AciI CCGC 3 cut(s) 39, 337, 500
AclWI GGATC 1 cut(s) 502
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 2 cut(s) 78, 499
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 3 cut(s) 147, 196, 467
AjnI CCWGG 4 cut(s) 75, 327, 510, 520
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AluBI AGCT 1 cut(s) 541
AluI AGCT 1 cut(s) 541
Alw26I GTCTC 1 cut(s) 40
AlwI GGATC 1 cut(s) 502
ApeKI GCWGC 2 cut(s) 303, 392
AspS9I GGNCC 1 cut(s) 557
AsuHPI GGTGA 3 cut(s) 85, 106, 385
AvaII GGWCC 1 cut(s) 557
BanI GGYRCC 1 cut(s) 234
BbvI GCAGC 2 cut(s) 315, 404
BccI CCATC 3 cut(s) 50, 80, 98
BciT130I CCWGG 4 cut(s) 77, 329, 512, 522
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 1 cut(s) 589
BfmI CTRYAG 1 cut(s) 84
BfuAI ACCTGC 1 cut(s) 545
BisI GCNGC 2 cut(s) 304, 393
BlsI GCNGC 2 cut(s) 305, 394
Bme1390I CCNGG 4 cut(s) 77, 329, 512, 522
Bme18I GGWCC 1 cut(s) 557
BmgT120I GGNCC 1 cut(s) 557
BmiI GGNNCC 2 cut(s) 10, 236
BmrFI CCNGG 4 cut(s) 77, 329, 512, 522
BmsI GCATC 2 cut(s) 58, 165
BsaJI CCNNGG 4 cut(s) 76, 230, 328, 521
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 499
Bse118I RCCGGY 1 cut(s) 237
Bse3DI GCAATG 1 cut(s) 342
BseBI CCWGG 4 cut(s) 77, 329, 512, 522
BseDI CCNNGG 4 cut(s) 76, 230, 328, 521
BseGI GGATG 4 cut(s) 61, 72, 172, 310
BseLI CCNNNNNNNGG 2 cut(s) 78, 499
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 2 cut(s) 176, 233
BseXI GCAGC 2 cut(s) 315, 404
Bsh1236I CGCG 1 cut(s) 41
BshNI GGYRCC 1 cut(s) 234
BsiSI CCGG 1 cut(s) 238
BslFI GGGAC 1 cut(s) 538
BslI CCNNNNNNNGG 2 cut(s) 78, 499
BsmAI GTCTC 1 cut(s) 40
BsmFI GGGAC 1 cut(s) 538
BsmI GAATGC 1 cut(s) 455
Bsp143I GATC 3 cut(s) 425, 436, 507
BspACI CCGC 3 cut(s) 39, 337, 500
BspCNI CTCAG 2 cut(s) 175, 232
BspFNI CGCG 1 cut(s) 41
BspLI GGNNCC 2 cut(s) 10, 236
BspMI ACCTGC 1 cut(s) 545
BspPI GGATC 1 cut(s) 502
BspT107I GGYRCC 1 cut(s) 234
BsrDI GCAATG 1 cut(s) 342
BsrFI RCCGGY 1 cut(s) 237
BssAI RCCGGY 1 cut(s) 237
BssECI CCNNGG 4 cut(s) 76, 230, 328, 521
BssMI GATC 3 cut(s) 425, 436, 507
BssT1I CCWWGG 1 cut(s) 230
Bst2UI CCWGG 4 cut(s) 77, 329, 512, 522
BstDEI CTNAG 3 cut(s) 162, 219, 378
BstF5I GGATG 4 cut(s) 61, 72, 172, 310
BstFNI CGCG 1 cut(s) 41
BstKTI GATC 3 cut(s) 428, 439, 510
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 3 cut(s) 425, 436, 507
BstNI CCWGG 4 cut(s) 77, 329, 512, 522
BstSCI CCNGG 4 cut(s) 75, 327, 510, 520
BstSFI CTRYAG 1 cut(s) 84
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 2 cut(s) 315, 404
BtsCI GGATG 4 cut(s) 61, 72, 172, 310
BveI ACCTGC 1 cut(s) 545
Cfr10I RCCGGY 1 cut(s) 237
Cfr13I GGNCC 1 cut(s) 557
CseI GACGC 1 cut(s) 30
Csp6I GTAC 1 cut(s) 289
CviAII CATG 3 cut(s) 32, 254, 585
CviJI RGCY 9 cut(s) 11, 89, 283, 306, 327, 477, 520, 541, 583
CviKI_1 RGCY 9 cut(s) 11, 89, 283, 306, 327, 477, 520, 541, 583
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 3 cut(s) 162, 219, 378
DpnI GATC 3 cut(s) 427, 438, 509
DpnII GATC 3 cut(s) 425, 436, 507
EciI GGCGGA 1 cut(s) 515
Eco130I CCWWGG 1 cut(s) 230
Eco47I GGWCC 1 cut(s) 557
EcoO109I RGGNCCY 1 cut(s) 557
EcoRII CCWGG 4 cut(s) 75, 327, 510, 520
EcoT14I CCWWGG 1 cut(s) 230
EcoT22I ATGCAT 1 cut(s) 572
ErhI CCWWGG 1 cut(s) 230
FaeI CATG 3 cut(s) 35, 257, 588
FaqI GGGAC 1 cut(s) 538
FatI CATG 3 cut(s) 31, 253, 584
FblI GTMKAC 1 cut(s) 83
Fnu4HI GCNGC 2 cut(s) 304, 393
FokI GGATG 4 cut(s) 59, 68, 179, 297
Fsp4HI GCNGC 2 cut(s) 304, 393
FspBI CTAG 1 cut(s) 589
GluI GCNGC 2 cut(s) 304, 393
HapII CCGG 1 cut(s) 238
HgaI GACGC 1 cut(s) 30
Hin1II CATG 3 cut(s) 35, 257, 588
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HindIII AAGCTT 1 cut(s) 539
HinfI GANTC 3 cut(s) 60, 467, 487
HpaI GTTAAC 1 cut(s) 216
HpaII CCGG 1 cut(s) 238
HphI GGTGA 3 cut(s) 85, 106, 385
Hpy166II GTNNAC 3 cut(s) 84, 216, 262
Hpy188I TCNGA 1 cut(s) 430
Hpy188III TCNNGA 2 cut(s) 164, 484
Hpy8I GTNNAC 3 cut(s) 84, 216, 262
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 4 cut(s) 178, 392, 554, 570
HpyF3I CTNAG 3 cut(s) 162, 219, 378
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 3 cut(s) 35, 257, 588
KspAI GTTAAC 1 cut(s) 216
Kzo9I GATC 3 cut(s) 425, 436, 507
LmnI GCTCC 3 cut(s) 8, 352, 452
Lsp1109I GCAGC 2 cut(s) 315, 404
LweI GCATC 2 cut(s) 58, 165
MaeI CTAG 1 cut(s) 589
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 2 cut(s) 107, 373
MalI GATC 3 cut(s) 427, 438, 509
MboI GATC 3 cut(s) 425, 436, 507
MfeI CAATTG 1 cut(s) 103
MluCI AATT 4 cut(s) 46, 103, 321, 418
MlyI GAGTC 1 cut(s) 54
MnlI CCTC 6 cut(s) 22, 46, 364, 375, 490, 570
Mph1103I ATGCAT 1 cut(s) 572
MseI TTAA 4 cut(s) 215, 353, 564, 573
MslI CAYNNNNRTG 1 cut(s) 126
MspI CCGG 1 cut(s) 238
MspR9I CCNGG 4 cut(s) 77, 329, 512, 522
MunI CAATTG 1 cut(s) 103
Mva1269I GAATGC 1 cut(s) 455
MvaI CCWGG 4 cut(s) 77, 329, 512, 522
MvnI CGCG 1 cut(s) 41
NdeII GATC 3 cut(s) 425, 436, 507
NlaIII CATG 3 cut(s) 35, 257, 588
NlaIV GGNNCC 2 cut(s) 10, 236
NmuCI GTSAC 2 cut(s) 107, 373
NsiI ATGCAT 1 cut(s) 572
PctI GAATGC 1 cut(s) 455
PfeI GAWTC 2 cut(s) 467, 487
PflFI GACNNNGTC 1 cut(s) 32
PkrI GCNGC 2 cut(s) 305, 394
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
PpuMI RGGWCCY 1 cut(s) 557
Psp5II RGGWCCY 1 cut(s) 557
Psp6I CCWGG 4 cut(s) 75, 327, 510, 520
PspGI CCWGG 4 cut(s) 75, 327, 510, 520
PspN4I GGNNCC 2 cut(s) 10, 236
PspPI GGNCC 1 cut(s) 557
PspPPI RGGWCCY 1 cut(s) 557
PsyI GACNNNGTC 1 cut(s) 32
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
RseI CAYNNNNRTG 1 cut(s) 126
SaqAI TTAA 4 cut(s) 215, 353, 564, 573
SatI GCNGC 2 cut(s) 304, 393
Sau3AI GATC 3 cut(s) 425, 436, 507
Sau96I GGNCC 1 cut(s) 557
SchI GAGTC 1 cut(s) 54
ScrFI CCNGG 4 cut(s) 77, 329, 512, 522
SetI ASST 5 cut(s) 236, 375, 405, 543, 559
SfaNI GCATC 2 cut(s) 58, 165
SfcI CTRYAG 1 cut(s) 84
SinI GGWCC 1 cut(s) 557
SmiMI CAYNNNNRTG 1 cut(s) 126
Sse9I AATT 4 cut(s) 46, 103, 321, 418
SsiI CCGC 3 cut(s) 39, 337, 500
SspMI CTAG 1 cut(s) 589
StyD4I CCNGG 4 cut(s) 75, 327, 510, 520
StyI CCWWGG 1 cut(s) 230
TaiI ACGT 1 cut(s) 405
TaqII GACCGA 1 cut(s) 194
TasI AATT 4 cut(s) 46, 103, 321, 418
TfiI GAWTC 2 cut(s) 467, 487
Tru1I TTAA 4 cut(s) 215, 353, 564, 573
Tru9I TTAA 4 cut(s) 215, 353, 564, 573
TseFI GTSAC 2 cut(s) 107, 373
TseI GCWGC 2 cut(s) 303, 392
Tsp45I GTSAC 2 cut(s) 107, 373
TspDTI ATGAA 4 cut(s) 183, 331, 372, 461
TspGWI ACGGA 1 cut(s) 339
Tth111I GACNNNGTC 1 cut(s) 32
VpaK11BI GGWCC 1 cut(s) 557
XcmI CCANNNNNNNNNTGG 2 cut(s) 237, 518
XmiI GTMKAC 1 cut(s) 83
XspI CTAG 1 cut(s) 589
Zsp2I ATGCAT 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.