Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0003744

Physical Location & Seq

Genomic Coordinates Forward (+)

32573980 .. 32575961

Gene Structure 1982 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1188600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1326 bp

ATGGCAATGGAAGCTGCTTACAAGCTATTTGTGTTTATTTTATGTATTTTTATTGTTGCAATAACAATTCCGGCAACGGTGACGGCCAACATTGCGGTTTTCGACGAGCACTGGCAAGGTCGAGCTAAAGAGGCGAAGCAGGCCGCCAACAAAGCCTACAATAAAAATCCTGCAGAAGTCACTGGAAGTTTCAATAAGGAAGTCCACAACACCTTCGATAGCATGAACAATACGCGAAGGAACTTAAAAGAGAAGTACAAGGGTCCATGCGTGGCTACAAACCCTATTGATCGTTGCTGGAGATGTGATCCAAACTGGGAAACAAATCGCAAGAAGCTGGCTGATTGCGCCAAAGGATTTGGGCACAAGACGACAGGAGGCAAGGCTGGAGAGATATATGTGGTGACAGATAACTCCGACAATGACCTGGTGAACCCGAAACCAGGGACACTACGCCACGCTGTGATTCAAACTGGGCCACTCTGGATCATATTCGCTCACGACATGAAGATAAAGCTAAGCGAAGAGCTTATGGTGACGAGTGACAAGACGATTGATGCACGAGGTGCTAATGTGCACATTCAAGACGGTGGCCAAATTACATTACAGTTTGTGAAGAATGTGATCATCCATAACCTCCACATTCACGATAACAAGGCAGGAAATGGTGGCATGATTAGGGACTCGGTGAATCACTATGGACAACGCACACGTAGTGATGGTGATGGTATATCAATGTATGGAGCCTCAAACGTTTGGATAGACCATGTCTCTGCGTCAAATTGCGAGGATGGACTCATTGATGCCATCCAGGGGTCTACAGCCATCACCATATCCAATTGTCACTTCACCAATCATAATGATGTGATGTTATTTGGTTCAAGTGATAGCAACTCTCAGGATGAAGTGATGCAGATAACTATTGCTTTCAACCATTTCGGTCAGGGGTTAACTCAGCGTATGCCAAGGTGCCGGTGGGGGTTTTTCCACGTTGTAAACAACGACTACACACATTGGCTTATGTACGCTATTGGTGGCAGCCAACATCCTACCATAATTAGCCAGGGCAACCGCTTCATTGCTCCGTCAAATGAAGCAAGTAAAGAGGTGACTAAGAGGGAATATGCAGCAGAGAACGTGTGGAAATCGTGGAATTGGAGATCGGAGAACGATCTTATGGTGAATGGAGCATTCTTTATTGAATCTGGTAGCCCAATCAGGAATCTCCCAGAGGCCGATATGATCCAGGCAAAGCCTGGGACATTCGTGGCGAAGCTCACACGCTTTGCAGGTCCTCTTAAATGCATTAAAGGGAAGCCATGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00040 ko02024 map00040 map02024

Pfam Domains

Protein Families

Pec_lyase_C Pec_lyase_N

Protein Analysis

442

Amino Acids

49.11

Weight (kDa)

8.25

Isoelectric Point (pI)

33.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pec_lyase_N	PF04431	30 - 82	3.1e-20	Pectate lyase, N terminus
Pectate_lyase_4	PF00544	121 - 373	5.5e-25	Pectate lyase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003744)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01270 AT5G15110
fragaria_vesca	FvH4_5g15430 FvH4_6g49230
malus_domestica	MD06G1188600.v1.1 MD09G1047400.v1.1 MD14G1195000.v1.1 MD14G1195200.v1.1 MD17G1047500.v1.1
prunus_persica	Prupe.3G271600_v2.0.a1 Prupe.5G186900_v2.0.a1
pyrus_communis	pycom06g16740 pycom17g04230
rosa_chinensis	RchiOBHm_Chr2g0169091 RchiOBHm_Chr7g0180651
rosa_laevigata	RLG00000005246 RLG00000021833
rosa_multiflora	Rmu_co8469407.1_g000001 Rmu_co8473671.1_g000001 Rmu_ssc0000393.1_g000032
rosa_roxburghii	Rroxscaffold_2G00082470
rosa_rugosa	Rorug02G0536800 Rorug06G0434600
rosa_samantha	Rh2AG608700 Rh2BG621600 Rh2CG590300 Rh2DG632200 Rh7AG036000 Rh7BG035900 Rh7CG037500 Rh7DG036100
rosa_wichuraiana	Rw2G050470 Rw7G002960 Rw7G002970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1280
AccB1I	GGYRCC	1 cut(s)	969
AccI	GTMKAC	1 cut(s)	818
AccII	CGCG	1 cut(s)	235
AciI	CCGC	3 cut(s)	95, 144, 1072
AclI	AACGTT	1 cut(s)	753
AclWI	GGATC	3 cut(s)	302, 494, 1237
AcoI	YGGCCR	2 cut(s)	84, 592
AdeI	CACNNNGTG	3 cut(s)	463, 566, 716
AfaI	GTAC	2 cut(s)	257, 1025
AfiI	CCNNNNNNNGG	2 cut(s)	443, 813
AflIII	ACRYGT	2 cut(s)	710, 1137
AgsI	TTSAA	6 cut(s)	193, 470, 584, 882, 931, 1202
AjnI	CCWGG	6 cut(s)	426, 442, 810, 1062, 1245, 1255
AjuI	GAANNNNNNNTTGG	2 cut(s)	830, 862
AluBI	AGCT	7 cut(s)	14, 25, 125, 337, 517, 529, 1276
AluI	AGCT	7 cut(s)	14, 25, 125, 337, 517, 529, 1276
Alw21I	GWGCWC	2 cut(s)	111, 579
Alw26I	GTCTC	1 cut(s)	775
Alw44I	GTGCAC	1 cut(s)	575
AlwI	GGATC	3 cut(s)	302, 494, 1237
AoxI	GGCC	5 cut(s)	84, 141, 476, 592, 1233
ApaLI	GTGCAC	1 cut(s)	575
ApeKI	GCWGC	3 cut(s)	14, 1038, 1127
AspLEI	GCGC	1 cut(s)	350
AspS9I	GGNCC	3 cut(s)	263, 476, 1292
AvaII	GGWCC	2 cut(s)	263, 1292
BaeGI	GKGCMC	2 cut(s)	366, 579
BalI	TGGCCA	1 cut(s)	594
BanI	GGYRCC	1 cut(s)	969
BauI	CACGAG	1 cut(s)	561
Bbv12I	GWGCWC	2 cut(s)	111, 579
BbvI	GCAGC	2 cut(s)	1050, 1139
BccI	CCATC	5 cut(s)	713, 719, 785, 815, 833
BceAI	ACGGC	1 cut(s)	99
BciT130I	CCWGG	6 cut(s)	428, 444, 812, 1064, 1247, 1257
BclI	TGATCA	1 cut(s)	624
BcoDI	GTCTC	1 cut(s)	775
BfaI	CTAG	1 cut(s)	1324
BfmI	CTRYAG	2 cut(s)	171, 819
BfuAI	ACCTGC	1 cut(s)	1280
BisI	GCNGC	4 cut(s)	15, 144, 1039, 1128
BlpI	GCTNAGC	1 cut(s)	518
BlsI	GCNGC	4 cut(s)	16, 145, 1040, 1129
Bme1390I	CCNGG	6 cut(s)	428, 444, 812, 1064, 1247, 1257
Bme18I	GGWCC	2 cut(s)	263, 1292
BmgT120I	GGNCC	3 cut(s)	263, 476, 1292
BmiI	GGNNCC	3 cut(s)	264, 745, 971
BmrFI	CCNGG	6 cut(s)	428, 444, 812, 1064, 1247, 1257
BmrI	ACTGGG	2 cut(s)	325, 483
BmsI	GCATC	3 cut(s)	547, 793, 900
BmuI	ACTGGG	2 cut(s)	325, 483
BpmI	CTGGAG	2 cut(s)	319, 408
Bpu1102I	GCTNAGC	1 cut(s)	518
BsaAI	YACGTR	1 cut(s)	713
BsaJI	CCNNGG	5 cut(s)	443, 811, 965, 1063, 1256
Bsc4I	CCNNNNNNNGG	2 cut(s)	443, 813
Bse118I	RCCGGY	1 cut(s)	972
Bse1I	ACTGG	4 cut(s)	116, 187, 320, 478
Bse3DI	GCAATG	3 cut(s)	12, 90, 1077
BseBI	CCWGG	6 cut(s)	428, 444, 812, 1064, 1247, 1257
BseDI	CCNNGG	5 cut(s)	443, 811, 965, 1063, 1256
BseGI	GGATG	5 cut(s)	627, 796, 807, 907, 1045
BseLI	CCNNNNNNNGG	2 cut(s)	443, 813
BseMI	GCAATG	3 cut(s)	12, 90, 1077
BseMII	CTCAG	2 cut(s)	911, 968
BseNI	ACTGG	4 cut(s)	116, 187, 320, 478
BseSI	GKGCMC	2 cut(s)	366, 579
BseXI	GCAGC	2 cut(s)	1050, 1139
Bsh1236I	CGCG	1 cut(s)	235
BshFI	GGCC	5 cut(s)	86, 143, 478, 594, 1235
BshNI	GGYRCC	1 cut(s)	969
BsiHKAI	GWGCWC	2 cut(s)	111, 579
BsiSI	CCGG	2 cut(s)	71, 973
BslFI	GGGAC	3 cut(s)	460, 695, 1273
BslI	CCNNNNNNNGG	2 cut(s)	443, 813
BsmAI	GTCTC	1 cut(s)	775
BsmFI	GGGAC	3 cut(s)	460, 695, 1273
BsmI	GAATGC	1 cut(s)	1190
BsnI	GGCC	5 cut(s)	86, 143, 478, 594, 1235
Bsp1286I	GDGCHC	3 cut(s)	111, 366, 579
Bsp143I	GATC	7 cut(s)	289, 307, 486, 624, 1160, 1171, 1242
Bsp1720I	GCTNAGC	1 cut(s)	518
BspACI	CCGC	3 cut(s)	95, 144, 1072
BspANI	GGCC	5 cut(s)	86, 143, 478, 594, 1235
BspCNI	CTCAG	2 cut(s)	910, 967
BspFNI	CGCG	1 cut(s)	235
BspLI	GGNNCC	3 cut(s)	264, 745, 971
BspMAI	CTGCAG	1 cut(s)	175
BspMI	ACCTGC	1 cut(s)	1280
BspPI	GGATC	3 cut(s)	302, 494, 1237
BspQI	GCTCTTC	1 cut(s)	519
BspT107I	GGYRCC	1 cut(s)	969
BsrDI	GCAATG	3 cut(s)	12, 90, 1077
BsrFI	RCCGGY	1 cut(s)	972
BsrI	ACTGG	4 cut(s)	116, 187, 320, 478
BssAI	RCCGGY	1 cut(s)	972
BssECI	CCNNGG	5 cut(s)	443, 811, 965, 1063, 1256
BssMI	GATC	7 cut(s)	289, 307, 486, 624, 1160, 1171, 1242
BssSI	CACGAG	1 cut(s)	561
BssT1I	CCWWGG	1 cut(s)	965
Bst2BI	CACGAG	1 cut(s)	561
Bst2UI	CCWGG	6 cut(s)	428, 444, 812, 1064, 1247, 1257
Bst4CI	ACNGT	3 cut(s)	79, 590, 609
Bst6I	CTCTTC	1 cut(s)	519
BstAPI	GCANNNNNTGC	1 cut(s)	566
BstBAI	YACGTR	1 cut(s)	713
BstC8I	GCNNGC	2 cut(s)	141, 339
BstDEI	CTNAG	4 cut(s)	518, 897, 954, 1113
BstF5I	GGATG	5 cut(s)	627, 796, 807, 907, 1045
BstFNI	CGCG	1 cut(s)	235
BstHHI	GCGC	1 cut(s)	350
BstKTI	GATC	7 cut(s)	292, 310, 489, 627, 1163, 1174, 1245
BstMAI	GTCTC	1 cut(s)	775
BstMBI	GATC	7 cut(s)	289, 307, 486, 624, 1160, 1171, 1242
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 92, 131, 140, 152, 347, 566
BstNI	CCWGG	6 cut(s)	428, 444, 812, 1064, 1247, 1257
BstSCI	CCNGG	6 cut(s)	426, 442, 810, 1062, 1245, 1255
BstSFI	CTRYAG	2 cut(s)	171, 819
BstSLI	GKGCMC	2 cut(s)	366, 579
BstUI	CGCG	1 cut(s)	235
BstV1I	GCAGC	2 cut(s)	1050, 1139
BsuRI	GGCC	5 cut(s)	86, 143, 478, 594, 1235
BtsCI	GGATG	5 cut(s)	627, 796, 807, 907, 1045
BtsIMutI	CAGTG	2 cut(s)	109, 180
BveI	ACCTGC	1 cut(s)	1280
Cac8I	GCNNGC	2 cut(s)	141, 339
CfoI	GCGC	1 cut(s)	350
Cfr10I	RCCGGY	1 cut(s)	972
Cfr13I	GGNCC	3 cut(s)	263, 476, 1292
CseI	GACGC	1 cut(s)	765
CsiI	ACCWGGT	1 cut(s)	426
Csp6I	GTAC	2 cut(s)	256, 1024
CviAII	CATG	6 cut(s)	223, 267, 505, 673, 767, 1320
CviQI	GTAC	2 cut(s)	256, 1024
DdeI	CTNAG	4 cut(s)	518, 897, 954, 1113
DpnI	GATC	7 cut(s)	291, 309, 488, 626, 1162, 1173, 1244
DpnII	GATC	7 cut(s)	289, 307, 486, 624, 1160, 1171, 1242
DraIII	CACNNNGTG	3 cut(s)	463, 566, 716
EaeI	YGGCCR	2 cut(s)	84, 592
Eam1104I	CTCTTC	1 cut(s)	519
EarI	CTCTTC	1 cut(s)	519
Eco130I	CCWWGG	1 cut(s)	965
Eco47I	GGWCC	2 cut(s)	263, 1292
EcoO109I	RGGNCCY	1 cut(s)	1292
EcoRII	CCWGG	6 cut(s)	426, 442, 810, 1062, 1245, 1255
EcoT14I	CCWWGG	1 cut(s)	965
EcoT22I	ATGCAT	1 cut(s)	1307
ErhI	CCWWGG	1 cut(s)	965
FaeI	CATG	6 cut(s)	226, 270, 508, 676, 770, 1323
FaqI	GGGAC	3 cut(s)	460, 695, 1273
FatI	CATG	6 cut(s)	222, 266, 504, 672, 766, 1319
FbaI	TGATCA	1 cut(s)	624
FblI	GTMKAC	1 cut(s)	818
Fnu4HI	GCNGC	4 cut(s)	15, 144, 1039, 1128
FokI	GGATG	5 cut(s)	614, 794, 803, 914, 1032
Fsp4HI	GCNGC	4 cut(s)	15, 144, 1039, 1128
FspBI	CTAG	1 cut(s)	1324
GlaI	GCGC	1 cut(s)	349
GluI	GCNGC	4 cut(s)	15, 144, 1039, 1128
GsuI	CTGGAG	2 cut(s)	319, 408
HaeIII	GGCC	5 cut(s)	86, 143, 478, 594, 1235
HapII	CCGG	2 cut(s)	71, 973
HgaI	GACGC	1 cut(s)	765
HhaI	GCGC	1 cut(s)	350
Hin1II	CATG	6 cut(s)	226, 270, 508, 676, 770, 1323
Hin6I	GCGC	1 cut(s)	348
HinP1I	GCGC	1 cut(s)	348
HincII	GTYRAC	1 cut(s)	951
HindII	GTYRAC	1 cut(s)	951
HinfI	GANTC	6 cut(s)	466, 683, 691, 795, 1202, 1222
HpaI	GTTAAC	1 cut(s)	951
HpaII	CCGG	2 cut(s)	71, 973
Hpy166II	GTNNAC	6 cut(s)	205, 433, 577, 819, 951, 997
Hpy188I	TCNGA	2 cut(s)	418, 1165
Hpy188III	TCNNGA	6 cut(s)	484, 500, 584, 647, 899, 1219
Hpy8I	GTNNAC	6 cut(s)	205, 433, 577, 819, 951, 997
Hpy99I	CGWCG	1 cut(s)	107
HpyAV	CCTTC	2 cut(s)	223, 231
HpyCH4III	ACNGT	3 cut(s)	79, 590, 609
HpyCH4IV	ACGT	4 cut(s)	712, 753, 990, 1137
HpyCH4V	TGCA	8 cut(s)	59, 173, 560, 577, 913, 1127, 1289, 1305
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 92, 131, 140, 152, 347, 566
HpyF3I	CTNAG	4 cut(s)	518, 897, 954, 1113
HpySE526I	ACGT	4 cut(s)	712, 753, 990, 1137
Hsp92II	CATG	6 cut(s)	226, 270, 508, 676, 770, 1323
HspAI	GCGC	1 cut(s)	348
Ksp22I	TGATCA	1 cut(s)	624
KspAI	GTTAAC	1 cut(s)	951
Kzo9I	GATC	7 cut(s)	289, 307, 486, 624, 1160, 1171, 1242
LguI	GCTCTTC	1 cut(s)	519
LmnI	GCTCC	3 cut(s)	743, 1087, 1187
Lsp1109I	GCAGC	2 cut(s)	1050, 1139
LweI	GCATC	3 cut(s)	547, 793, 900
MabI	ACCWGGT	1 cut(s)	426
MaeI	CTAG	1 cut(s)	1324
MaeII	ACGT	4 cut(s)	712, 753, 990, 1137
MaeIII	GTNAC	7 cut(s)	79, 178, 403, 535, 542, 842, 1108
MalI	GATC	7 cut(s)	291, 309, 488, 626, 1162, 1173, 1244
MboI	GATC	7 cut(s)	289, 307, 486, 624, 1160, 1171, 1242
MboII	GAAGA	3 cut(s)	520, 536, 628
MfeI	CAATTG	1 cut(s)	838
MhlI	GDGCHC	3 cut(s)	111, 366, 579
MlsI	TGGCCA	1 cut(s)	594
MluCI	AATT	6 cut(s)	66, 597, 781, 838, 1056, 1153
MluNI	TGGCCA	1 cut(s)	594
MlyI	GAGTC	2 cut(s)	677, 789
MmeI	TCCRAC	1 cut(s)	441
Mox20I	TGGCCA	1 cut(s)	594
Mph1103I	ATGCAT	1 cut(s)	1307
MscI	TGGCCA	1 cut(s)	594
MseI	TTAA	4 cut(s)	245, 950, 1299, 1308
MslI	CAYNNNNRTG	1 cut(s)	861
Msp20I	TGGCCA	1 cut(s)	594
MspI	CCGG	2 cut(s)	71, 973
MspR9I	CCNGG	6 cut(s)	428, 444, 812, 1064, 1247, 1257
MunI	CAATTG	1 cut(s)	838
Mva1269I	GAATGC	1 cut(s)	1190
MvaI	CCWGG	6 cut(s)	428, 444, 812, 1064, 1247, 1257
MvnI	CGCG	1 cut(s)	235
MwoI	GCNNNNNNNGC	7 cut(s)	11, 92, 131, 140, 152, 347, 566
NdeII	GATC	7 cut(s)	289, 307, 486, 624, 1160, 1171, 1242
NlaIII	CATG	6 cut(s)	226, 270, 508, 676, 770, 1323
NlaIV	GGNNCC	3 cut(s)	264, 745, 971
NmuCI	GTSAC	7 cut(s)	79, 178, 403, 535, 542, 842, 1108
NsiI	ATGCAT	1 cut(s)	1307
PciSI	GCTCTTC	1 cut(s)	519
PctI	GAATGC	1 cut(s)	1190
PfeI	GAWTC	4 cut(s)	466, 691, 1202, 1222
PflFI	GACNNNGTC	1 cut(s)	767
PkrI	GCNGC	4 cut(s)	16, 145, 1040, 1129
PleI	GAGTC	2 cut(s)	677, 789
PpsI	GAGTC	2 cut(s)	677, 789
Ppu21I	YACGTR	1 cut(s)	713
PpuMI	RGGWCCY	1 cut(s)	1292
Psp1406I	AACGTT	1 cut(s)	753
Psp5II	RGGWCCY	1 cut(s)	1292
Psp6I	CCWGG	6 cut(s)	426, 442, 810, 1062, 1245, 1255
PspGI	CCWGG	6 cut(s)	426, 442, 810, 1062, 1245, 1255
PspN4I	GGNNCC	3 cut(s)	264, 745, 971
PspPI	GGNCC	3 cut(s)	263, 476, 1292
PspPPI	RGGWCCY	1 cut(s)	1292
PstI	CTGCAG	1 cut(s)	175
PsyI	GACNNNGTC	1 cut(s)	767
RsaI	GTAC	2 cut(s)	257, 1025
RsaNI	GTAC	2 cut(s)	256, 1024
RseI	CAYNNNNRTG	1 cut(s)	861
SapI	GCTCTTC	1 cut(s)	519
SaqAI	TTAA	4 cut(s)	245, 950, 1299, 1308
SatI	GCNGC	4 cut(s)	15, 144, 1039, 1128
Sau3AI	GATC	7 cut(s)	289, 307, 486, 624, 1160, 1171, 1242
Sau96I	GGNCC	3 cut(s)	263, 476, 1292
SchI	GAGTC	2 cut(s)	677, 789
ScrFI	CCNGG	6 cut(s)	428, 444, 812, 1064, 1247, 1257
SduI	GDGCHC	3 cut(s)	111, 366, 579
SexAI	ACCWGGT	1 cut(s)	426
SfaNI	GCATC	3 cut(s)	547, 793, 900
SfcI	CTRYAG	2 cut(s)	171, 819
SinI	GGWCC	2 cut(s)	263, 1292
SmiMI	CAYNNNNRTG	1 cut(s)	861
Sse9I	AATT	6 cut(s)	66, 597, 781, 838, 1056, 1153
SsiI	CCGC	3 cut(s)	95, 144, 1072
SspMI	CTAG	1 cut(s)	1324
StyD4I	CCNGG	6 cut(s)	426, 442, 810, 1062, 1245, 1255
StyI	CCWWGG	1 cut(s)	965
TaaI	ACNGT	3 cut(s)	79, 590, 609
TaiI	ACGT	4 cut(s)	715, 756, 993, 1140
TaqI	TCGA	3 cut(s)	102, 121, 216
TaqII	GACCGA	1 cut(s)	929
TasI	AATT	6 cut(s)	66, 597, 781, 838, 1056, 1153
TatI	WGTACW	1 cut(s)	255
TauI	GCSGC	1 cut(s)	146
TfiI	GAWTC	4 cut(s)	466, 691, 1202, 1222
Tru1I	TTAA	4 cut(s)	245, 950, 1299, 1308
Tru9I	TTAA	4 cut(s)	245, 950, 1299, 1308
TscAI	CASTG	2 cut(s)	116, 187
TseFI	GTSAC	7 cut(s)	79, 178, 403, 535, 542, 842, 1108
TseI	GCWGC	3 cut(s)	14, 1038, 1127
Tsp45I	GTSAC	7 cut(s)	79, 178, 403, 535, 542, 842, 1108
TspDTI	ATGAA	5 cut(s)	239, 521, 918, 1066, 1107
TspGWI	ACGGA	1 cut(s)	1074
TspRI	CASTG	2 cut(s)	116, 187
Tth111I	GACNNNGTC	1 cut(s)	767
VneI	GTGCAC	1 cut(s)	575
VpaK11BI	GGWCC	2 cut(s)	263, 1292
XcmI	CCANNNNNNNNNTGG	2 cut(s)	972, 1253
XmiI	GTMKAC	1 cut(s)	818
XspI	CTAG	1 cut(s)	1324
Zsp2I	ATGCAT	1 cut(s)	1307

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.