Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0003744

Physical Location & Seq

Genomic Coordinates Forward (+)

3456455 .. 3458842

Gene Structure 2388 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1047500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1365 bp

ATGGCGTCGAAGCTCAATTTGGCCCTTCTTATGATATCTTTCTGCATGCTGGTTCCAAGCTTGAAGGCAGAGACACAAAATGACACCCTCAACATTGCTGAGTTCGACTCTGTATGGCAGCAACGAGCCCTGAAAGCTCAGAAGGCAGCTCTCAAGGCCTACCAACCCAACCCGGAACAAGTAACCGAGGACTTCAACGAAAGCGTTGAAATGACCATCATTGAGGACGACCACGACGATGATCATGCTGCGGGCAACAGCACAGGGAGGCATCTGTTAGGCAAACGTTACACGGGCCCTTGCAAGGCCACAAACCCTATCGACCGCTGCTGGCGGTGCCAGGCCAACTGGGCTGACAACCGCAAGCATCTGGCCAGCTGCGTGAAAGGCTTCGGCCGCAAGACACGCGGAGGCAAGAAAGGGGCTTACTACATCGTCACCGACTCTTCCGACGACAATATCCATGACCCTAAGCCCGGAACCCTACGTCACGCCGTGATCCAAAAGCAACCCTTGTGGATCATATTTGCACGCAGCATGGTCATCAGGCTGACCCAGGAGCTCATAGTAACAAGCCACAAGACAATTGACGCACGCGGGGCAAATGTGCATATTGCTCATGGCGCTGGGATCACACTCCAGTTCGTGCAGAACGTGATTATTCATGGCCTCCACATCCACGACACTGTACCGGGCAATGGAGGTATGATCAGGGACTCCGTGGATCACATCGGGATTCGCACGCATAGCGACGGAGATGGTATCTCCATCTACGGATCGTCTAACATTTGGCTCGACCATCTTTCTATGTGGAATTGCGCCGATGGGCTCATCGACGCCATTCAAGGGTCCACTGCTATAACCATATCCAACAGCCACTTCACCCGTCACAACGACGTGATGCTTTTCGGAGCTAGCGATGGGTTTTCTGGGGATGCAATCATGCAGATAACGGTTGCATTTAACCATTTCGGCAAGGGATTGATACAGAGAATGCCAAGATGCAGATGGGGATTTATCCATGTTGTGAACAATGACTACACACATTGGCTCATGTATGCCATCGGAGGAAGCTCTCACCCCACAATTATCAGCCAGGGCAACCGATTCATCGCCCCACCGAACCAGGCTGCAAAAGAAGTGACGAAGAGAGACTATGCACCGGAGAGCGTATGGAAGAGCTGGCAATGGAGATCAGAAGGAGATCTTATGATAAACGGAGCATTCTTTGTTCAATCTGGTGTGCCTAGCAAGAACCATGGATTTGGAAGGTTAGACGTCATGAAGGCAAGGCCAGGTACATACGTCACAAGGCTCACACGCTTTGCAGGTTCTCTCAACTGCAAGGTTGGCCACCCATGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00040 ko02024 map00040 map02024

Pfam Domains

Protein Families

Pec_lyase_C Pec_lyase_N

Protein Analysis

455

Amino Acids

50.36

Weight (kDa)

8.77

Isoelectric Point (pI)

32.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pec_lyase_N	PF04431	31 - 92	1.7e-18	Pectate lyase, N terminus
Pectate_lyase_4	PF00544	189 - 384	2.5e-18	Pectate lyase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003744)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01270 AT5G15110
fragaria_vesca	FvH4_5g15430 FvH4_6g49230
malus_domestica	MD06G1188600.v1.1 MD09G1047400.v1.1 MD14G1195000.v1.1 MD14G1195200.v1.1 MD17G1047500.v1.1
prunus_persica	Prupe.3G271600_v2.0.a1 Prupe.5G186900_v2.0.a1
pyrus_communis	pycom06g16740 pycom17g04230
rosa_chinensis	RchiOBHm_Chr2g0169091 RchiOBHm_Chr7g0180651
rosa_laevigata	RLG00000005246 RLG00000021833
rosa_multiflora	Rmu_co8469407.1_g000001 Rmu_co8473671.1_g000001 Rmu_ssc0000393.1_g000032
rosa_roxburghii	Rroxscaffold_2G00082470
rosa_rugosa	Rorug02G0536800 Rorug06G0434600
rosa_samantha	Rh2AG608700 Rh2BG621600 Rh2CG590300 Rh2DG632200 Rh7AG036000 Rh7BG035900 Rh7CG037500 Rh7DG036100
rosa_wichuraiana	Rw2G050470 Rw7G002960 Rw7G002970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1281
Acc36I	ACCTGC	1 cut(s)	1319
AccB1I	GGYRCC	1 cut(s)	336
AccII	CGCG	2 cut(s)	408, 597
AciI	CCGC	7 cut(s)	251, 325, 334, 361, 397, 408, 597
AclI	AACGTT	1 cut(s)	286
AclWI	GGATC	5 cut(s)	493, 527, 638, 732, 784
AcoI	YGGCCR	3 cut(s)	372, 394, 1351
AcyI	GRCGYC	3 cut(s)	5, 837, 1278
AdeI	CACNNNGTG	1 cut(s)	496
AfaI	GTAC	2 cut(s)	690, 1300
AfiI	CCNNNNNNNGG	4 cut(s)	304, 476, 698, 846
AgsI	TTSAA	5 cut(s)	64, 196, 209, 845, 1235
AjiI	CACGTC	1 cut(s)	898
AjnI	CCWGG	5 cut(s)	339, 555, 1095, 1125, 1294
AjuI	GAANNNNNNNTTGG	3 cut(s)	34, 863, 895
AluBI	AGCT	9 cut(s)	13, 60, 137, 149, 378, 562, 914, 1074, 1182
AluI	AGCT	9 cut(s)	13, 60, 137, 149, 378, 562, 914, 1074, 1182
Alw21I	GWGCWC	1 cut(s)	564
Alw26I	GTCTC	2 cut(s)	65, 1146
AlwI	GGATC	5 cut(s)	493, 527, 638, 732, 784
ApaI	GGGCCC	1 cut(s)	299
ApeKI	GCWGC	7 cut(s)	118, 146, 248, 327, 378, 534, 1130
Asp700I	GAANNNNTTC	1 cut(s)	389
AspLEI	GCGC	2 cut(s)	626, 821
AspS9I	GGNCC	4 cut(s)	22, 295, 296, 849
AsuC2I	CCSGG	3 cut(s)	173, 477, 693
AsuHPI	GGTGA	3 cut(s)	430, 874, 1070
AsuNHI	GCTAGC	1 cut(s)	914
AvaII	GGWCC	1 cut(s)	849
BaeGI	GKGCMC	1 cut(s)	299
BaeI	ACNNNNGTAYC	2 cut(s)	1290, 1323
BalI	TGGCCA	2 cut(s)	374, 1353
BanI	GGYRCC	1 cut(s)	336
BanII	GRGCYC	4 cut(s)	130, 299, 564, 831
Bbv12I	GWGCWC	1 cut(s)	564
BbvI	GCAGC	7 cut(s)	130, 158, 235, 314, 365, 546, 1117
BccI	CCATC	8 cut(s)	224, 752, 776, 807, 818, 914, 1002, 1070
BceAI	ACGGC	1 cut(s)	479
BcgI	CGANNNNNNTGC	2 cut(s)	227, 261
BciT130I	CCWGG	5 cut(s)	341, 557, 1097, 1127, 1296
BclI	TGATCA	2 cut(s)	241, 708
BcnI	CCSGG	3 cut(s)	173, 477, 693
BcoDI	GTCTC	2 cut(s)	65, 1146
BfaI	CTAG	3 cut(s)	915, 1248, 1363
BfoI	RGCGCY	1 cut(s)	627
BfuAI	ACCTGC	1 cut(s)	1319
BglI	GCCNNNNNGGC	1 cut(s)	350
BglII	AGATCT	1 cut(s)	1204
BisI	GCNGC	8 cut(s)	119, 147, 249, 328, 379, 397, 535, 1131
BlsI	GCNGC	8 cut(s)	120, 148, 250, 329, 380, 398, 536, 1132
Bme1390I	CCNGG	8 cut(s)	173, 341, 477, 557, 693, 1097, 1127, 1296
Bme18I	GGWCC	1 cut(s)	849
BmgBI	CACGTC	1 cut(s)	898
BmgT120I	GGNCC	4 cut(s)	22, 295, 296, 849
BmiI	GGNNCC	5 cut(s)	54, 297, 338, 481, 850
BmrFI	CCNGG	8 cut(s)	173, 341, 477, 557, 693, 1097, 1127, 1296
BmrI	ACTGGG	1 cut(s)	358
BmsI	GCATC	5 cut(s)	280, 376, 891, 925, 992
BmtI	GCTAGC	1 cut(s)	918
BmuI	ACTGGG	1 cut(s)	358
BplI	GAGNNNNNCTC	2 cut(s)	92, 124
BpmI	CTGGAG	1 cut(s)	623
Bpu10I	CCTNAGC	1 cut(s)	471
BpuEI	CTTGAG	1 cut(s)	137
BpuMI	CCSGG	3 cut(s)	173, 477, 693
BsaHI	GRCGYC	3 cut(s)	5, 837, 1278
BsaJI	CCNNGG	5 cut(s)	186, 555, 720, 1096, 1258
BsaWI	WCCGGW	1 cut(s)	1162
Bsc4I	CCNNNNNNNGG	4 cut(s)	304, 476, 698, 846
Bse1I	ACTGG	2 cut(s)	353, 640
Bse3DI	GCAATG	3 cut(s)	93, 703, 1193
BseBI	CCWGG	5 cut(s)	341, 557, 1097, 1127, 1296
BseDI	CCNNGG	5 cut(s)	186, 555, 720, 1096, 1258
BseGI	GGATG	2 cut(s)	675, 940
BseLI	CCNNNNNNNGG	4 cut(s)	304, 476, 698, 846
BseMI	GCAATG	3 cut(s)	93, 703, 1193
BseMII	CTCAG	2 cut(s)	90, 152
BseNI	ACTGG	2 cut(s)	353, 640
BseSI	GKGCMC	1 cut(s)	299
BseX3I	CGGCCG	1 cut(s)	394
BseXI	GCAGC	7 cut(s)	130, 158, 235, 314, 365, 546, 1117
BseYI	CCCAGC	1 cut(s)	626
BsgI	GTGCAG	1 cut(s)	668
Bsh1236I	CGCG	2 cut(s)	408, 597
Bsh1285I	CGRYCG	2 cut(s)	325, 397
BshNI	GGYRCC	1 cut(s)	336
BsiEI	CGRYCG	2 cut(s)	325, 397
BsiHKAI	GWGCWC	1 cut(s)	564
BsiSI	CCGG	4 cut(s)	173, 477, 692, 1163
BslFI	GGGAC	1 cut(s)	728
BslI	CCNNNNNNNGG	4 cut(s)	304, 476, 698, 846
BsmAI	GTCTC	2 cut(s)	65, 1146
BsmFI	GGGAC	1 cut(s)	728
BsmI	GAATGC	2 cut(s)	999, 1223
Bsp120I	GGGCCC	1 cut(s)	295
Bsp1286I	GDGCHC	4 cut(s)	130, 299, 564, 831
Bsp143I	GATC	9 cut(s)	241, 498, 519, 630, 708, 724, 776, 1193, 1204
Bsp19I	CCATGG	1 cut(s)	1258
BspACI	CCGC	7 cut(s)	251, 325, 334, 361, 397, 408, 597
BspCNI	CTCAG	2 cut(s)	91, 151
BspFNI	CGCG	2 cut(s)	408, 597
BspHI	TCATGA	1 cut(s)	1281
BspLI	GGNNCC	5 cut(s)	54, 297, 338, 481, 850
BspMI	ACCTGC	1 cut(s)	1319
BspOI	GCTAGC	1 cut(s)	918
BspPI	GGATC	5 cut(s)	493, 527, 638, 732, 784
BspQI	GCTCTTC	1 cut(s)	1172
BspT107I	GGYRCC	1 cut(s)	336
BsrDI	GCAATG	3 cut(s)	93, 703, 1193
BsrI	ACTGG	2 cut(s)	353, 640
BssECI	CCNNGG	5 cut(s)	186, 555, 720, 1096, 1258
BssMI	GATC	9 cut(s)	241, 498, 519, 630, 708, 724, 776, 1193, 1204
BssNI	GRCGYC	3 cut(s)	5, 837, 1278
BssT1I	CCWWGG	1 cut(s)	1258
Bst2UI	CCWGG	5 cut(s)	341, 557, 1097, 1127, 1296
Bst4CI	ACNGT	2 cut(s)	688, 955
Bst6I	CTCTTC	3 cut(s)	451, 1142, 1172
BstACI	GRCGYC	3 cut(s)	5, 837, 1278
BstDEI	CTNAG	3 cut(s)	99, 138, 471
BstDSI	CCRYGG	2 cut(s)	720, 1258
BstF5I	GGATG	2 cut(s)	675, 940
BstFNI	CGCG	2 cut(s)	408, 597
BstH2I	RGCGCY	1 cut(s)	627
BstHHI	GCGC	2 cut(s)	626, 821
BstKTI	GATC	9 cut(s)	244, 501, 522, 633, 711, 727, 779, 1196, 1207
BstMAI	GTCTC	2 cut(s)	65, 1146
BstMBI	GATC	9 cut(s)	241, 498, 519, 630, 708, 724, 776, 1193, 1204
BstMCI	CGRYCG	2 cut(s)	325, 397
BstNI	CCWGG	5 cut(s)	341, 557, 1097, 1127, 1296
BstNSI	RCATGY	1 cut(s)	49
BstSCI	CCNGG	8 cut(s)	171, 339, 475, 555, 691, 1095, 1125, 1294
BstSLI	GKGCMC	1 cut(s)	299
BstUI	CGCG	2 cut(s)	408, 597
BstV1I	GCAGC	7 cut(s)	130, 158, 235, 314, 365, 546, 1117
BstX2I	RGATCY	1 cut(s)	1204
BstXI	CCANNNNNNTGG	1 cut(s)	1265
BstYI	RGATCY	1 cut(s)	1204
BstZI	CGGCCG	1 cut(s)	394
BtgI	CCRYGG	2 cut(s)	720, 1258
BtgZI	GCGATG	2 cut(s)	933, 1096
BtrI	CACGTC	1 cut(s)	898
BtsCI	GGATG	2 cut(s)	675, 940
BtsI	GCAGTG	1 cut(s)	852
BtsIMutI	CAGTG	2 cut(s)	684, 852
BveI	ACCTGC	1 cut(s)	1319
CciI	TCATGA	1 cut(s)	1281
CfoI	GCGC	2 cut(s)	626, 821
Cfr13I	GGNCC	4 cut(s)	22, 295, 296, 849
CseI	GACGC	2 cut(s)	599, 845
Csp6I	GTAC	2 cut(s)	689, 1299
CspCI	CAANNNNNGTGG	2 cut(s)	497, 532
CviQI	GTAC	2 cut(s)	689, 1299
DdeI	CTNAG	3 cut(s)	99, 138, 471
DpnI	GATC	9 cut(s)	243, 500, 521, 632, 710, 726, 778, 1195, 1206
DpnII	GATC	9 cut(s)	241, 498, 519, 630, 708, 724, 776, 1193, 1204
DraIII	CACNNNGTG	1 cut(s)	496
EaeI	YGGCCR	3 cut(s)	372, 394, 1351
EagI	CGGCCG	1 cut(s)	394
Eam1104I	CTCTTC	3 cut(s)	451, 1142, 1172
EarI	CTCTTC	3 cut(s)	451, 1142, 1172
Ecl136II	GAGCTC	1 cut(s)	562
EclXI	CGGCCG	1 cut(s)	394
Eco130I	CCWWGG	1 cut(s)	1258
Eco147I	AGGCCT	1 cut(s)	158
Eco24I	GRGCYC	4 cut(s)	130, 299, 564, 831
Eco32I	GATATC	1 cut(s)	36
Eco47I	GGWCC	1 cut(s)	849
Eco52I	CGGCCG	1 cut(s)	394
Eco53kI	GAGCTC	1 cut(s)	562
EcoICRI	GAGCTC	1 cut(s)	562
EcoO109I	RGGNCCY	1 cut(s)	296
EcoRII	CCWGG	5 cut(s)	339, 555, 1095, 1125, 1294
EcoRV	GATATC	1 cut(s)	36
EcoT14I	CCWWGG	1 cut(s)	1258
EcoT38I	GRGCYC	4 cut(s)	130, 299, 564, 831
ErhI	CCWWGG	1 cut(s)	1258
FalI	AAGNNNNNCTT	4 cut(s)	497, 529, 1191, 1223
FaqI	GGGAC	1 cut(s)	728
FauI	CCCGC	2 cut(s)	244, 590
FbaI	TGATCA	2 cut(s)	241, 708
Fnu4HI	GCNGC	8 cut(s)	119, 147, 249, 328, 379, 397, 535, 1131
FokI	GGATG	2 cut(s)	662, 947
FriOI	GRGCYC	4 cut(s)	130, 299, 564, 831
Fsp4HI	GCNGC	8 cut(s)	119, 147, 249, 328, 379, 397, 535, 1131
FspBI	CTAG	3 cut(s)	915, 1248, 1363
GlaI	GCGC	2 cut(s)	625, 820
GluI	GCNGC	8 cut(s)	119, 147, 249, 328, 379, 397, 535, 1131
GsaI	CCCAGC	1 cut(s)	630
GsuI	CTGGAG	1 cut(s)	623
HaeII	RGCGCY	1 cut(s)	627
HapII	CCGG	4 cut(s)	173, 477, 692, 1163
HgaI	GACGC	2 cut(s)	599, 845
HhaI	GCGC	2 cut(s)	626, 821
Hin1I	GRCGYC	3 cut(s)	5, 837, 1278
Hin6I	GCGC	2 cut(s)	624, 819
HinP1I	GCGC	2 cut(s)	624, 819
HindIII	AAGCTT	1 cut(s)	58
HinfI	GANTC	5 cut(s)	107, 443, 716, 736, 1107
HpaII	CCGG	4 cut(s)	173, 477, 692, 1163
HphI	GGTGA	3 cut(s)	430, 874, 1070
Hpy166II	GTNNAC	2 cut(s)	852, 1030
Hpy188I	TCNGA	5 cut(s)	141, 451, 911, 1067, 1198
Hpy188III	TCNNGA	2 cut(s)	733, 1282
Hpy8I	GTNNAC	2 cut(s)	852, 1030
Hpy99I	CGWCG	6 cut(s)	10, 239, 455, 755, 839, 899
HpyAV	CCTTC	6 cut(s)	35, 58, 136, 1193, 1263, 1279
HpyCH4III	ACNGT	2 cut(s)	688, 955
HpyCH4IV	ACGT	6 cut(s)	286, 487, 654, 897, 1278, 1305
HpyF3I	CTNAG	3 cut(s)	99, 138, 471
HpySE526I	ACGT	6 cut(s)	286, 487, 654, 897, 1278, 1305
Hsp92I	GRCGYC	3 cut(s)	5, 837, 1278
HspAI	GCGC	2 cut(s)	624, 819
Ksp22I	TGATCA	2 cut(s)	241, 708
Kzo9I	GATC	9 cut(s)	241, 498, 519, 630, 708, 724, 776, 1193, 1204
LguI	GCTCTTC	1 cut(s)	1172
LmnI	GCTCC	3 cut(s)	559, 911, 1220
Lsp1109I	GCAGC	7 cut(s)	130, 158, 235, 314, 365, 546, 1117
LweI	GCATC	5 cut(s)	280, 376, 891, 925, 992
MaeI	CTAG	3 cut(s)	915, 1248, 1363
MaeII	ACGT	6 cut(s)	286, 487, 654, 897, 1278, 1305
MaeIII	GTNAC	8 cut(s)	181, 287, 436, 488, 568, 887, 1141, 1306
MalI	GATC	9 cut(s)	243, 500, 521, 632, 710, 726, 778, 1195, 1206
MboI	GATC	9 cut(s)	241, 498, 519, 630, 708, 724, 776, 1193, 1204
MboII	GAAGA	3 cut(s)	438, 1159, 1189
MfeI	CAATTG	1 cut(s)	585
MflI	RGATCY	1 cut(s)	1204
MhlI	GDGCHC	4 cut(s)	130, 299, 564, 831
MlsI	TGGCCA	2 cut(s)	374, 1353
MluCI	AATT	4 cut(s)	16, 585, 814, 1086
MluNI	TGGCCA	2 cut(s)	374, 1353
MlyI	GAGTC	3 cut(s)	101, 437, 710
MmeI	TCCRAC	2 cut(s)	474, 894
MnlI	CCTC	8 cut(s)	98, 181, 217, 261, 404, 680, 695, 1061
Mox20I	TGGCCA	2 cut(s)	374, 1353
MroXI	GAANNNNTTC	1 cut(s)	389
MscI	TGGCCA	2 cut(s)	374, 1353
MseI	TTAA	1 cut(s)	963
MslI	CAYNNNNRTG	1 cut(s)	237
Msp20I	TGGCCA	2 cut(s)	374, 1353
MspA1I	CMGCKG	2 cut(s)	327, 378
MspI	CCGG	4 cut(s)	173, 477, 692, 1163
MspR9I	CCNGG	8 cut(s)	173, 341, 477, 557, 693, 1097, 1127, 1296
MunI	CAATTG	1 cut(s)	585
Mva1269I	GAATGC	2 cut(s)	999, 1223
MvaI	CCWGG	5 cut(s)	341, 557, 1097, 1127, 1296
MvnI	CGCG	2 cut(s)	408, 597
NciI	CCSGG	3 cut(s)	173, 477, 693
NcoI	CCATGG	1 cut(s)	1258
NdeII	GATC	9 cut(s)	241, 498, 519, 630, 708, 724, 776, 1193, 1204
NheI	GCTAGC	1 cut(s)	914
NlaIV	GGNNCC	5 cut(s)	54, 297, 338, 481, 850
NmuCI	GTSAC	5 cut(s)	436, 488, 887, 1141, 1306
NspI	RCATGY	1 cut(s)	49
PaeI	GCATGC	1 cut(s)	49
PagI	TCATGA	1 cut(s)	1281
PceI	AGGCCT	1 cut(s)	158
PciSI	GCTCTTC	1 cut(s)	1172
PcsI	WCGNNNNNNNCGW	3 cut(s)	234, 651, 915
PctI	GAATGC	2 cut(s)	999, 1223
PdmI	GAANNNNTTC	1 cut(s)	389
PfeI	GAWTC	2 cut(s)	736, 1107
PkrI	GCNGC	8 cut(s)	120, 148, 250, 329, 380, 398, 536, 1132
PleI	GAGTC	3 cut(s)	101, 437, 710
PpsI	GAGTC	3 cut(s)	101, 437, 710
Psp124BI	GAGCTC	1 cut(s)	564
Psp1406I	AACGTT	1 cut(s)	286
Psp6I	CCWGG	5 cut(s)	339, 555, 1095, 1125, 1294
PspFI	CCCAGC	1 cut(s)	626
PspGI	CCWGG	5 cut(s)	339, 555, 1095, 1125, 1294
PspN4I	GGNNCC	5 cut(s)	54, 297, 338, 481, 850
PspOMI	GGGCCC	1 cut(s)	295
PspPI	GGNCC	4 cut(s)	22, 295, 296, 849
PsrI	GAACNNNNNNTAC	2 cut(s)	1022, 1054
PsuI	RGATCY	1 cut(s)	1204
PvuII	CAGCTG	1 cut(s)	378
RsaI	GTAC	2 cut(s)	690, 1300
RsaNI	GTAC	2 cut(s)	689, 1299
RseI	CAYNNNNRTG	1 cut(s)	237
SacI	GAGCTC	1 cut(s)	564
SapI	GCTCTTC	1 cut(s)	1172
SaqAI	TTAA	1 cut(s)	963
SatI	GCNGC	8 cut(s)	119, 147, 249, 328, 379, 397, 535, 1131
Sau3AI	GATC	9 cut(s)	241, 498, 519, 630, 708, 724, 776, 1193, 1204
Sau96I	GGNCC	4 cut(s)	22, 295, 296, 849
SchI	GAGTC	3 cut(s)	101, 437, 710
ScrFI	CCNGG	8 cut(s)	173, 341, 477, 557, 693, 1097, 1127, 1296
SduI	GDGCHC	4 cut(s)	130, 299, 564, 831
SfaNI	GCATC	5 cut(s)	280, 376, 891, 925, 992
SinI	GGWCC	1 cut(s)	849
SmiMI	CAYNNNNRTG	1 cut(s)	237
SmlI	CTYRAG	1 cut(s)	152
SmoI	CTYRAG	1 cut(s)	152
SphI	GCATGC	1 cut(s)	49
Sse9I	AATT	4 cut(s)	16, 585, 814, 1086
SseBI	AGGCCT	1 cut(s)	158
SsiI	CCGC	7 cut(s)	251, 325, 334, 361, 397, 408, 597
SspMI	CTAG	3 cut(s)	915, 1248, 1363
SstI	GAGCTC	1 cut(s)	564
StuI	AGGCCT	1 cut(s)	158
StyD4I	CCNGG	8 cut(s)	171, 339, 475, 555, 691, 1095, 1125, 1294
StyI	CCWWGG	1 cut(s)	1258
TaaI	ACNGT	2 cut(s)	688, 955
TaiI	ACGT	6 cut(s)	289, 490, 657, 900, 1281, 1308
TaqI	TCGA	5 cut(s)	8, 105, 321, 795, 834
TasI	AATT	4 cut(s)	16, 585, 814, 1086
TauI	GCSGC	1 cut(s)	399
TfiI	GAWTC	2 cut(s)	736, 1107
Tru1I	TTAA	1 cut(s)	963
Tru9I	TTAA	1 cut(s)	963
TscAI	CASTG	2 cut(s)	691, 859
TseFI	GTSAC	5 cut(s)	436, 488, 887, 1141, 1306
TseI	GCWGC	7 cut(s)	118, 146, 248, 327, 378, 534, 1130
Tsp45I	GTSAC	5 cut(s)	436, 488, 887, 1141, 1306
TspDTI	ATGAA	3 cut(s)	653, 1099, 1298
TspGWI	ACGGA	4 cut(s)	709, 768, 789, 1233
TspRI	CASTG	2 cut(s)	691, 859
VpaK11BI	GGWCC	1 cut(s)	849
XceI	RCATGY	1 cut(s)	49
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1005
XmnI	GAANNNNTTC	1 cut(s)	389
XspI	CTAG	3 cut(s)	915, 1248, 1363
ZraI	GACGTC	1 cut(s)	1279

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.