MD14G1195000.v1.1

Pectate lyase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003744
Physical Location & Seq
Forward (+)
28590204 .. 28590901
698 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1195000.v1.1.491

Sequence Viewer

Length: 414 bp
ATGGAAGCTAATTACAAGCTAAATGTGTTTATTTTATGTATTTTTATTATTACAATCACAATTCCGGCAACGGTGAGGGCCAACATTGCGGATTTCGACGCGCACTGGCAAGGTCGAGCAAAAGAAGCGAAGCAAGCCGCCAACGAAGCCTACAATAAAAATCCTGCACAAGTCACTGAAAGTTTCAACAAGGAAGTCCACGACACGTTTGATGCCGCGAATAAAACGAGAAGGAACTTGAAACAGAAGTACAAGGGTCCATGCCTGGCTACAAACCCTAGCTTGGTACAGTGTCGAATTCCTCATCAAGCTCATCCGGGTCGACCGTATTCGCTTGTGAAAGCCGGATATCCGTTGCAGGCCTTGTTAGTAGATTTGGTTGAGGGGCAGAGGCAAATGCACACTAGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C Pec_lyase_N

Protein Analysis

138

Amino Acids

15.47

Weight (kDa)

9.51

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pec_lyase_N PF04431 28 - 80 2e-20 Pectate lyase, N terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 322
AccII CGCG 2 cut(s) 101, 218
AciI CCGC 3 cut(s) 89, 138, 216
AcsI RAATTY 1 cut(s) 297
AfaI GTAC 2 cut(s) 251, 288
AfiI CCNNNNNNNGG 1 cut(s) 283
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 2 cut(s) 187, 241
AjnI CCWGG 1 cut(s) 264
AluBI AGCT 4 cut(s) 8, 19, 282, 311
AluI AGCT 4 cut(s) 8, 19, 282, 311
AoxI GGCC 2 cut(s) 78, 360
ApoI RAATTY 1 cut(s) 297
AspLEI GCGC 1 cut(s) 103
AspS9I GGNCC 2 cut(s) 78, 257
AsuC2I CCSGG 1 cut(s) 318
AsuHPI GGTGA 1 cut(s) 85
AvaII GGWCC 1 cut(s) 257
BciT130I CCWGG 1 cut(s) 266
BcnI CCSGG 1 cut(s) 318
BfaI CTAG 2 cut(s) 279, 405
BisI GCNGC 2 cut(s) 138, 216
BlsI GCNGC 2 cut(s) 139, 217
Bme1390I CCNGG 2 cut(s) 266, 318
Bme18I GGWCC 1 cut(s) 257
BmgT120I GGNCC 2 cut(s) 78, 257
BmiI GGNNCC 1 cut(s) 258
BmrFI CCNGG 2 cut(s) 266, 318
BmsI GCATC 1 cut(s) 202
BpuMI CCSGG 1 cut(s) 318
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse1I ACTGG 1 cut(s) 110
Bse3DI GCAATG 1 cut(s) 84
BseBI CCWGG 1 cut(s) 266
BseGI GGATG 1 cut(s) 313
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMI GCAATG 1 cut(s) 84
BseNI ACTGG 1 cut(s) 110
BsgI GTGCAG 1 cut(s) 150
Bsh1236I CGCG 2 cut(s) 101, 218
Bsh1285I CGRYCG 1 cut(s) 326
BshFI GGCC 2 cut(s) 80, 362
BsiEI CGRYCG 1 cut(s) 326
BsiSI CCGG 3 cut(s) 65, 317, 345
BslI CCNNNNNNNGG 1 cut(s) 283
BsnI GGCC 2 cut(s) 80, 362
BspACI CCGC 3 cut(s) 89, 138, 216
BspANI GGCC 2 cut(s) 80, 362
BspFNI CGCG 2 cut(s) 101, 218
BspLI GGNNCC 1 cut(s) 258
BsrDI GCAATG 1 cut(s) 84
BsrI ACTGG 1 cut(s) 110
Bst2UI CCWGG 1 cut(s) 266
Bst4CI ACNGT 3 cut(s) 73, 291, 327
BstC8I GCNNGC 2 cut(s) 135, 360
BstF5I GGATG 1 cut(s) 313
BstFNI CGCG 2 cut(s) 101, 218
BstHHI GCGC 1 cut(s) 103
BstMCI CGRYCG 1 cut(s) 326
BstMWI GCNNNNNNNGC 4 cut(s) 86, 125, 134, 146
BstNI CCWGG 1 cut(s) 266
BstSCI CCNGG 2 cut(s) 264, 316
BstUI CGCG 2 cut(s) 101, 218
BsuRI GGCC 2 cut(s) 80, 362
BtsCI GGATG 1 cut(s) 313
BtsIMutI CAGTG 3 cut(s) 103, 174, 296
Cac8I GCNNGC 2 cut(s) 135, 360
CfoI GCGC 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 78, 257
CseI GACGC 1 cut(s) 107
Csp6I GTAC 2 cut(s) 250, 287
CviAII CATG 1 cut(s) 261
CviQI GTAC 2 cut(s) 250, 287
Eco147I AGGCCT 1 cut(s) 362
Eco32I GATATC 1 cut(s) 350
Eco47I GGWCC 1 cut(s) 257
EcoRI GAATTC 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 264
EcoRV GATATC 1 cut(s) 350
FaeI CATG 1 cut(s) 264
FaiI YATR 2 cut(s) 37, 262
FatI CATG 1 cut(s) 260
FblI GTMKAC 1 cut(s) 322
Fnu4HI GCNGC 2 cut(s) 138, 216
FokI GGATG 1 cut(s) 300
Fsp4HI GCNGC 2 cut(s) 138, 216
FspBI CTAG 2 cut(s) 279, 405
GlaI GCGC 1 cut(s) 102
GluI GCNGC 2 cut(s) 138, 216
HaeIII GGCC 2 cut(s) 80, 362
HapII CCGG 3 cut(s) 65, 317, 345
HgaI GACGC 1 cut(s) 107
HhaI GCGC 1 cut(s) 103
Hin1II CATG 1 cut(s) 264
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HincII GTYRAC 1 cut(s) 323
HindII GTYRAC 1 cut(s) 323
HpaII CCGG 3 cut(s) 65, 317, 345
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 2 cut(s) 199, 323
Hpy8I GTNNAC 2 cut(s) 199, 323
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 225
HpyCH4III ACNGT 3 cut(s) 73, 291, 327
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 3 cut(s) 167, 358, 400
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 125, 134, 146
HpySE526I ACGT 1 cut(s) 206
Hsp92II CATG 1 cut(s) 264
HspAI GCGC 1 cut(s) 101
LpnPI CCDG 8 cut(s) 78, 91, 177, 251, 278, 330, 344, 358
LweI GCATC 1 cut(s) 202
MaeI CTAG 2 cut(s) 279, 405
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 1 cut(s) 172
MluCI AATT 3 cut(s) 10, 60, 297
MnlI CCTC 4 cut(s) 69, 312, 376, 384
MspI CCGG 3 cut(s) 65, 317, 345
MspR9I CCNGG 2 cut(s) 266, 318
MvaI CCWGG 1 cut(s) 266
MvnI CGCG 2 cut(s) 101, 218
MwoI GCNNNNNNNGC 4 cut(s) 86, 125, 134, 146
NciI CCSGG 1 cut(s) 318
NlaIII CATG 1 cut(s) 264
NlaIV GGNNCC 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 172
PceI AGGCCT 1 cut(s) 362
PkrI GCNGC 2 cut(s) 139, 217
Psp6I CCWGG 1 cut(s) 264
PspGI CCWGG 1 cut(s) 264
PspN4I GGNNCC 1 cut(s) 258
PspPI GGNCC 2 cut(s) 78, 257
RsaI GTAC 2 cut(s) 251, 288
RsaNI GTAC 2 cut(s) 250, 287
SalI GTCGAC 1 cut(s) 321
SatI GCNGC 2 cut(s) 138, 216
Sau96I GGNCC 2 cut(s) 78, 257
ScrFI CCNGG 2 cut(s) 266, 318
SetI ASST 6 cut(s) 10, 21, 115, 209, 284, 313
SfaNI GCATC 1 cut(s) 202
SinI GGWCC 1 cut(s) 257
Sse9I AATT 3 cut(s) 10, 60, 297
SseBI AGGCCT 1 cut(s) 362
SsiI CCGC 3 cut(s) 89, 138, 216
SspMI CTAG 2 cut(s) 279, 405
StuI AGGCCT 1 cut(s) 362
StyD4I CCNGG 2 cut(s) 264, 316
TaaI ACNGT 3 cut(s) 73, 291, 327
TaiI ACGT 1 cut(s) 209
TaqI TCGA 4 cut(s) 96, 115, 295, 322
TasI AATT 3 cut(s) 10, 60, 297
TatI WGTACW 1 cut(s) 249
TauI GCSGC 2 cut(s) 140, 218
TscAI CASTG 3 cut(s) 110, 181, 296
TseFI GTSAC 1 cut(s) 172
Tsp45I GTSAC 1 cut(s) 172
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 3 cut(s) 110, 181, 296
VpaK11BI GGWCC 1 cut(s) 257
XapI RAATTY 1 cut(s) 297
XmiI GTMKAC 1 cut(s) 322
XspI CTAG 2 cut(s) 279, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.