Rw7G002970

Pectate lyase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0003744
Physical Location & Seq
Forward (+)
2426023 .. 2427628
1606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002970.1

Sequence Viewer

Length: 1263 bp
ATGGCAATGGAAGCTGATCACAAGCTGAAGCTGCTATTATTATGTCTTCTGATGACAATAACAATTCCGGCATTGAAGGCCAACATCGGGGAATTTGATGAGAGTGGGAGAGGCGACAGGAAGCAGCCGAAAAGTCTGCGAAAGCGGACACTGGAGGGTCTTAACGACACAAGAAGGAACTTGAGACACCATTATGATGGTCCATGCCAAGTTACAAACCCAATCGACGCTTGCTGGAGATGCGATCCAAATTGGGATCAAGATCGGCAGAAGCTAGCCGATTGTGCTCTTGGCTTTGGACATGAGACAACCGGAGGCAAAGGTGGACGTATCTATACCGTCACTGATCCTAGTGACGATAACGTGCTCGAACCAGAAGAGGGTACATTGCGGTGGGCTGTGATTCAACCTGAACCGCTCTGGATCATATTTAAAGATGACATGAACATAGAACTGAAAGAAGAGCTCATGGTCACTAGTAACAAGACCATTGATGCAAGGGGGTGTAATGTGCACATTCAGGGTGGTGCGCAAATTACTCTGCAGTATGTGCAGAACATCATTATCTGTAACCTTCACGTTCGTGACACTGTTTCCAAGGAAGGTGGCATGGTTAGGGACTCGACGGGTCACTTTGGACTACGTACAGCCAGCGATGGTGATGGAATATCCTTGTTTGGTGCCTCAAACGTTTGGATAGACCATGTTTCTGCCTCAAATTGCGCCGATGGACTCATCGATGTTGTTGCAGGGTCTACTGCCATTACCATATCCAACTCTCACTTCACCGATCATAATGATGTGATGCTGTTTGGTTCAAGCGATAGCTACTATAAGGATGAACTTATGCAGATAACTCTAGCTTTCAACCATTTCGGACAGGGATTGATACAGAGGATGCCAAGGTGCAGATTTGGTTTTTTCCATGTTGTGAACAATGACTACACTCACTGGATTATGTATGCCATCGGTGGCAGCAGCCATCCTACCATACTAAGCCAGGGAAATCGCTACATTGCTCCAGAAAATGCTGCAGCTAAAGAGATATGTAATAGAGTCAACACAGCAAAGAGTGAGTGGAGTAAATGGAGTTGGACATCAGAAGGAGATCTTTTCCTGAATGGAGCGACTTTTACTGAATCTGGAAGCCCGATTCGGAATATCCCACAAGACGATATGATAACTGCAAAGCCTGGGACATTCGTAACCACGCTCACAATGTTTGCCGGTCCTCTTAAATGTGATGAAGGCAAGCCATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C Pec_lyase_N

Protein Analysis

420

Amino Acids

46.62

Weight (kDa)

5.6

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 153 - 355 8.6e-20 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 531
AccB1I GGYRCC 1 cut(s) 680
AccBSI CCGCTC 1 cut(s) 418
AccI GTMKAC 1 cut(s) 755
AciI CCGC 3 cut(s) 145, 391, 416
AclI AACGTT 1 cut(s) 690
AclWI GGATC 4 cut(s) 239, 264, 341, 431
AcsI RAATTY 1 cut(s) 92
AcuI CTGAAG 1 cut(s) 47
AfaI GTAC 2 cut(s) 385, 646
AfiI CCNNNNNNNGG 2 cut(s) 87, 380
AgsI TTSAA 4 cut(s) 76, 407, 819, 868
AhlI ACTAGT 1 cut(s) 476
AjnI CCWGG 2 cut(s) 999, 1192
AjuI GAANNNNNNNTTGG 2 cut(s) 767, 799
AleI CACNNNNGTG 1 cut(s) 582
AluBI AGCT 8 cut(s) 14, 25, 31, 274, 466, 828, 863, 1037
AluI AGCT 8 cut(s) 14, 25, 31, 274, 466, 828, 863, 1037
Alw21I GWGCWC 4 cut(s) 289, 369, 468, 516
Alw26I GTCTC 2 cut(s) 178, 299
Alw44I GTGCAC 1 cut(s) 512
AlwI GGATC 4 cut(s) 239, 264, 341, 431
AoxI GGCC 1 cut(s) 78
ApaLI GTGCAC 1 cut(s) 512
ApeKI GCWGC 6 cut(s) 31, 124, 975, 978, 1031, 1034
ApoI RAATTY 1 cut(s) 92
AspLEI GCGC 2 cut(s) 532, 725
AspS9I GGNCC 2 cut(s) 200, 1229
AsuHPI GGTGA 2 cut(s) 671, 778
AsuNHI GCTAGC 1 cut(s) 274
AvaII GGWCC 2 cut(s) 200, 1229
BaeGI GKGCMC 1 cut(s) 516
BanI GGYRCC 1 cut(s) 680
BanII GRGCYC 1 cut(s) 468
BbsI GAAGAC 1 cut(s) 38
Bbv12I GWGCWC 4 cut(s) 289, 369, 468, 516
BbvI GCAGC 6 cut(s) 18, 136, 987, 990, 1018, 1046
BccI CCATC 6 cut(s) 191, 650, 656, 722, 974, 990
BcgI CGANNNNNNTGC 4 cut(s) 118, 152, 728, 762
BciT130I CCWGG 2 cut(s) 1001, 1194
BclI TGATCA 1 cut(s) 16
BcoDI GTCTC 2 cut(s) 178, 299
BcuI ACTAGT 1 cut(s) 476
BfaI CTAG 4 cut(s) 275, 351, 477, 860
BfmI CTRYAG 2 cut(s) 542, 1032
BglII AGATCT 1 cut(s) 1108
BisI GCNGC 6 cut(s) 32, 125, 976, 979, 1032, 1035
BlsI GCNGC 6 cut(s) 33, 126, 977, 980, 1033, 1036
Bme1390I CCNGG 2 cut(s) 1001, 1194
Bme18I GGWCC 2 cut(s) 200, 1229
BmgT120I GGNCC 2 cut(s) 200, 1229
BmiI GGNNCC 1 cut(s) 682
BmrFI CCNGG 2 cut(s) 1001, 1194
BmsI GCATC 4 cut(s) 230, 484, 795, 888
BmtI GCTAGC 1 cut(s) 278
BpiI GAAGAC 1 cut(s) 38
BpmI CTGGAG 3 cut(s) 173, 256, 1005
BpuEI CTTGAG 1 cut(s) 202
Bsa29I ATCGAT 1 cut(s) 738
BsaAI YACGTR 1 cut(s) 644
BsaBI GATNNNNATC 1 cut(s) 261
BsaJI CCNNGG 4 cut(s) 597, 902, 1000, 1193
BsaWI WCCGGW 1 cut(s) 311
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 380
Bse118I RCCGGY 1 cut(s) 1226
Bse1I ACTGG 2 cut(s) 156, 956
Bse3DI GCAATG 3 cut(s) 12, 386, 1014
Bse8I GATNNNNATC 1 cut(s) 261
BseBI CCWGG 2 cut(s) 1001, 1194
BseCI ATCGAT 1 cut(s) 738
BseDI CCNNGG 4 cut(s) 597, 902, 1000, 1193
BseGI GGATG 3 cut(s) 844, 903, 982
BseJI GATNNNNATC 1 cut(s) 261
BseLI CCNNNNNNNGG 2 cut(s) 87, 380
BseMI GCAATG 3 cut(s) 12, 386, 1014
BseNI ACTGG 2 cut(s) 156, 956
BseSI GKGCMC 1 cut(s) 516
BseXI GCAGC 6 cut(s) 18, 136, 987, 990, 1018, 1046
BsgI GTGCAG 2 cut(s) 572, 928
BshFI GGCC 1 cut(s) 80
BshNI GGYRCC 1 cut(s) 680
BshVI ATCGAT 1 cut(s) 738
BsiHKAI GWGCWC 4 cut(s) 289, 369, 468, 516
BsiSI CCGG 3 cut(s) 68, 312, 1227
BslFI GGGAC 2 cut(s) 632, 1210
BslI CCNNNNNNNGG 2 cut(s) 87, 380
BsmAI GTCTC 2 cut(s) 178, 299
BsmFI GGGAC 2 cut(s) 632, 1210
BsnI GGCC 1 cut(s) 80
Bsp1286I GDGCHC 4 cut(s) 289, 369, 468, 516
Bsp143I GATC 8 cut(s) 16, 244, 256, 262, 346, 423, 790, 1108
BspACI CCGC 3 cut(s) 145, 391, 416
BspANI GGCC 1 cut(s) 80
BspDI ATCGAT 1 cut(s) 738
BspLI GGNNCC 1 cut(s) 682
BspMAI CTGCAG 2 cut(s) 546, 1036
BspOI GCTAGC 1 cut(s) 278
BspPI GGATC 4 cut(s) 239, 264, 341, 431
BspQI GCTCTTC 1 cut(s) 456
BspT107I GGYRCC 1 cut(s) 680
BsrBI CCGCTC 1 cut(s) 418
BsrDI GCAATG 3 cut(s) 12, 386, 1014
BsrFI RCCGGY 1 cut(s) 1226
BsrI ACTGG 2 cut(s) 156, 956
BssAI RCCGGY 1 cut(s) 1226
BssECI CCNNGG 4 cut(s) 597, 902, 1000, 1193
BssMI GATC 8 cut(s) 16, 244, 256, 262, 346, 423, 790, 1108
BssT1I CCWWGG 2 cut(s) 597, 902
Bst2UI CCWGG 2 cut(s) 1001, 1194
Bst4CI ACNGT 2 cut(s) 340, 592
Bst6I CTCTTC 2 cut(s) 372, 456
BstAPI GCANNNNNTGC 1 cut(s) 550
BstBAI YACGTR 1 cut(s) 644
BstC8I GCNNGC 4 cut(s) 232, 276, 652, 1253
BstDEI CTNAG 1 cut(s) 995
BstF5I GGATG 3 cut(s) 844, 903, 982
BstHHI GCGC 2 cut(s) 532, 725
BstKTI GATC 8 cut(s) 19, 247, 259, 265, 349, 426, 793, 1111
BstMAI GTCTC 2 cut(s) 178, 299
BstMBI GATC 8 cut(s) 16, 244, 256, 262, 346, 423, 790, 1108
BstMWI GCNNNNNNNGC 6 cut(s) 11, 31, 77, 240, 284, 550
BstNI CCWGG 2 cut(s) 1001, 1194
BstSCI CCNGG 2 cut(s) 999, 1192
BstSFI CTRYAG 2 cut(s) 542, 1032
BstSLI GKGCMC 1 cut(s) 516
BstSNI TACGTA 1 cut(s) 644
BstV1I GCAGC 6 cut(s) 18, 136, 987, 990, 1018, 1046
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 1 cut(s) 1108
BstXI CCANNNNNNTGG 1 cut(s) 197
BstYI RGATCY 1 cut(s) 1108
Bsu15I ATCGAT 1 cut(s) 738
BsuRI GGCC 1 cut(s) 80
BsuTUI ATCGAT 1 cut(s) 738
BtgZI GCGATG 1 cut(s) 669
BtsCI GGATG 3 cut(s) 844, 903, 982
BtsIMutI CAGTG 4 cut(s) 149, 342, 588, 949
Cac8I GCNNGC 4 cut(s) 232, 276, 652, 1253
CfoI GCGC 2 cut(s) 532, 725
Cfr10I RCCGGY 1 cut(s) 1226
Cfr13I GGNCC 2 cut(s) 200, 1229
ClaI ATCGAT 1 cut(s) 738
CseI GACGC 1 cut(s) 236
Csp6I GTAC 2 cut(s) 384, 645
CspCI CAANNNNNGTGG 2 cut(s) 586, 621
CviAII CATG 8 cut(s) 204, 302, 442, 469, 610, 704, 926, 1257
CviQI GTAC 2 cut(s) 384, 645
DdeI CTNAG 1 cut(s) 995
DpnI GATC 8 cut(s) 18, 246, 258, 264, 348, 425, 792, 1110
DpnII GATC 8 cut(s) 16, 244, 256, 262, 346, 423, 790, 1108
DraI TTTAAA 1 cut(s) 433
Eam1104I CTCTTC 2 cut(s) 372, 456
EarI CTCTTC 2 cut(s) 372, 456
Ecl136II GAGCTC 1 cut(s) 466
Eco105I TACGTA 1 cut(s) 644
Eco130I CCWWGG 2 cut(s) 597, 902
Eco24I GRGCYC 1 cut(s) 468
Eco47I GGWCC 2 cut(s) 200, 1229
Eco53kI GAGCTC 1 cut(s) 466
Eco57I CTGAAG 1 cut(s) 47
EcoICRI GAGCTC 1 cut(s) 466
EcoRII CCWGG 2 cut(s) 999, 1192
EcoT14I CCWWGG 2 cut(s) 597, 902
EcoT38I GRGCYC 1 cut(s) 468
ErhI CCWWGG 2 cut(s) 597, 902
FaeI CATG 8 cut(s) 207, 305, 445, 472, 613, 707, 929, 1260
FalI AAGNNNNNCTT 2 cut(s) 1095, 1127
FaqI GGGAC 2 cut(s) 632, 1210
FatI CATG 8 cut(s) 203, 301, 441, 468, 609, 703, 925, 1256
FbaI TGATCA 1 cut(s) 16
FblI GTMKAC 1 cut(s) 755
Fnu4HI GCNGC 6 cut(s) 32, 125, 976, 979, 1032, 1035
FokI GGATG 3 cut(s) 851, 910, 969
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 6 cut(s) 32, 125, 976, 979, 1032, 1035
FspBI CTAG 4 cut(s) 275, 351, 477, 860
FspI TGCGCA 1 cut(s) 531
GlaI GCGC 2 cut(s) 531, 724
GluI GCNGC 6 cut(s) 32, 125, 976, 979, 1032, 1035
GsuI CTGGAG 3 cut(s) 173, 256, 1005
HaeIII GGCC 1 cut(s) 80
HapII CCGG 3 cut(s) 68, 312, 1227
HgaI GACGC 1 cut(s) 236
HhaI GCGC 2 cut(s) 532, 725
Hin1II CATG 8 cut(s) 207, 305, 445, 472, 613, 707, 929, 1260
Hin6I GCGC 2 cut(s) 530, 723
HinP1I GCGC 2 cut(s) 530, 723
HincII GTYRAC 1 cut(s) 1060
HindII GTYRAC 1 cut(s) 1060
HinfI GANTC 6 cut(s) 403, 620, 732, 1056, 1139, 1153
HpaII CCGG 3 cut(s) 68, 312, 1227
HphI GGTGA 2 cut(s) 671, 778
Hpy166II GTNNAC 5 cut(s) 326, 514, 756, 934, 1060
Hpy188I TCNGA 4 cut(s) 51, 878, 1102, 1158
Hpy188III TCNNGA 6 cut(s) 260, 421, 584, 1022, 1117, 1143
Hpy8I GTNNAC 5 cut(s) 326, 514, 756, 934, 1060
Hpy99I CGWCG 2 cut(s) 230, 628
HpyAV CCTTC 6 cut(s) 70, 168, 584, 596, 1097, 1241
HpyCH4III ACNGT 2 cut(s) 340, 592
HpyCH4IV ACGT 5 cut(s) 328, 363, 579, 643, 690
HpyCH4V TGCA 9 cut(s) 497, 514, 544, 553, 749, 850, 909, 1034, 1187
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 31, 77, 240, 284, 550
HpyF3I CTNAG 1 cut(s) 995
HpySE526I ACGT 5 cut(s) 328, 363, 579, 643, 690
Hsp92II CATG 8 cut(s) 207, 305, 445, 472, 613, 707, 929, 1260
HspAI GCGC 2 cut(s) 530, 723
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 8 cut(s) 16, 244, 256, 262, 346, 423, 790, 1108
LguI GCTCTTC 1 cut(s) 456
LmnI GCTCC 2 cut(s) 1024, 1124
Lsp1109I GCAGC 6 cut(s) 18, 136, 987, 990, 1018, 1046
LweI GCATC 4 cut(s) 230, 484, 795, 888
MaeI CTAG 4 cut(s) 275, 351, 477, 860
MaeII ACGT 5 cut(s) 328, 363, 579, 643, 690
MaeIII GTNAC 9 cut(s) 211, 340, 353, 472, 479, 569, 584, 629, 1204
MalI GATC 8 cut(s) 18, 246, 258, 264, 348, 425, 792, 1110
MbiI CCGCTC 1 cut(s) 418
MboI GATC 8 cut(s) 16, 244, 256, 262, 346, 423, 790, 1108
MboII GAAGA 3 cut(s) 38, 389, 473
MflI RGATCY 1 cut(s) 1108
MhlI GDGCHC 4 cut(s) 289, 369, 468, 516
MluCI AATT 5 cut(s) 63, 92, 250, 534, 718
MlyI GAGTC 3 cut(s) 614, 726, 1065
MmeI TCCRAC 2 cut(s) 798, 1073
MnlI CCTC 8 cut(s) 104, 148, 308, 373, 694, 724, 888, 1242
MseI TTAA 3 cut(s) 162, 432, 1236
MslI CAYNNNNRTG 5 cut(s) 192, 195, 391, 582, 798
MspI CCGG 3 cut(s) 68, 312, 1227
MspR9I CCNGG 2 cut(s) 1001, 1194
MvaI CCWGG 2 cut(s) 1001, 1194
MwoI GCNNNNNNNGC 6 cut(s) 11, 31, 77, 240, 284, 550
NdeII GATC 8 cut(s) 16, 244, 256, 262, 346, 423, 790, 1108
NheI GCTAGC 1 cut(s) 274
NlaIII CATG 8 cut(s) 207, 305, 445, 472, 613, 707, 929, 1260
NlaIV GGNNCC 1 cut(s) 682
NmuCI GTSAC 5 cut(s) 340, 353, 472, 584, 629
NsbI TGCGCA 1 cut(s) 531
OliI CACNNNNGTG 1 cut(s) 582
PciSI GCTCTTC 1 cut(s) 456
PfeI GAWTC 3 cut(s) 403, 1139, 1153
PkrI GCNGC 6 cut(s) 33, 126, 977, 980, 1033, 1036
PleI GAGTC 3 cut(s) 614, 726, 1064
PpsI GAGTC 3 cut(s) 614, 726, 1064
Ppu21I YACGTR 1 cut(s) 644
Psp124BI GAGCTC 1 cut(s) 468
Psp1406I AACGTT 1 cut(s) 690
Psp6I CCWGG 2 cut(s) 999, 1192
PspGI CCWGG 2 cut(s) 999, 1192
PspN4I GGNNCC 1 cut(s) 682
PspPI GGNCC 2 cut(s) 200, 1229
PsrI GAACNNNNNNTAC 2 cut(s) 926, 958
PstI CTGCAG 2 cut(s) 546, 1036
PsuI RGATCY 1 cut(s) 1108
RsaI GTAC 2 cut(s) 385, 646
RsaNI GTAC 2 cut(s) 384, 645
RseI CAYNNNNRTG 5 cut(s) 192, 195, 391, 582, 798
SacI GAGCTC 1 cut(s) 468
SapI GCTCTTC 1 cut(s) 456
SaqAI TTAA 3 cut(s) 162, 432, 1236
SatI GCNGC 6 cut(s) 32, 125, 976, 979, 1032, 1035
Sau3AI GATC 8 cut(s) 16, 244, 256, 262, 346, 423, 790, 1108
Sau96I GGNCC 2 cut(s) 200, 1229
SchI GAGTC 3 cut(s) 614, 726, 1065
ScrFI CCNGG 2 cut(s) 1001, 1194
SduI GDGCHC 4 cut(s) 289, 369, 468, 516
SfaNI GCATC 4 cut(s) 230, 484, 795, 888
SfcI CTRYAG 2 cut(s) 542, 1032
SinI GGWCC 2 cut(s) 200, 1229
SmiMI CAYNNNNRTG 5 cut(s) 192, 195, 391, 582, 798
SmlI CTYRAG 1 cut(s) 181
SmoI CTYRAG 1 cut(s) 181
SnaBI TACGTA 1 cut(s) 644
SpeI ACTAGT 1 cut(s) 476
Sse9I AATT 5 cut(s) 63, 92, 250, 534, 718
SsiI CCGC 3 cut(s) 145, 391, 416
SspMI CTAG 4 cut(s) 275, 351, 477, 860
SstI GAGCTC 1 cut(s) 468
StyD4I CCNGG 2 cut(s) 999, 1192
StyI CCWWGG 2 cut(s) 597, 902
TaaI ACNGT 2 cut(s) 340, 592
TaiI ACGT 5 cut(s) 331, 366, 582, 646, 693
TaqI TCGA 4 cut(s) 225, 369, 623, 738
TasI AATT 5 cut(s) 63, 92, 250, 534, 718
TfiI GAWTC 3 cut(s) 403, 1139, 1153
Tru1I TTAA 3 cut(s) 162, 432, 1236
Tru9I TTAA 3 cut(s) 162, 432, 1236
TscAI CASTG 4 cut(s) 156, 349, 595, 956
TseFI GTSAC 5 cut(s) 340, 353, 472, 584, 629
TseI GCWGC 6 cut(s) 31, 124, 975, 978, 1031, 1034
Tsp45I GTSAC 5 cut(s) 340, 353, 472, 584, 629
TspDTI ATGAA 3 cut(s) 458, 855, 1260
TspRI CASTG 4 cut(s) 156, 349, 595, 956
VneI GTGCAC 1 cut(s) 512
VpaK11BI GGWCC 2 cut(s) 200, 1229
XapI RAATTY 1 cut(s) 92
XmiI GTMKAC 1 cut(s) 755
XspI CTAG 4 cut(s) 275, 351, 477, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.