Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003744

Physical Location & Seq

Genomic Coordinates Forward (+)

28599295 .. 28601368

Gene Structure 2074 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1195200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

ATGGAAGCTAATTACACGCTAAATGTGTTTATTTTATGTATTTTTATTATTACAATCACAATTCCGGCAACGGTGAGGGCCAACATTGCGGATTTCGACGCGCACTGGCAAGGTCGAGCAAAAGAAGCGAAGCAAGCCGCCAACGAAGCCTACAATAAAAATCCTGCACAAGTCACTGAAAGTTTCAACAAGGAAGTCCACGACACGTTTGATGCCGCGAATAAAACGAGAAGGAACTTGAAACAGAAGTACAAGGGTCCATGCCTGGCTACAAACCCTATTGATCGTTGCTGGAGATGTGACCCAAACTGGGAAACAAATCGCAAGAAGCTCGCTGATTGCGCCAAAGGATTTGGGCACAAGACGACAGGAGGCAAGGCTGGAAAAATCTATGTGGTGACTGATAACTCTGACAATGACATGGTGAACCCAAAACCAGGGACACTACGCCACGCTGTGATACAAACTGGGCCACTTTGGATCATATTCGCTCGAGACATGAGAATAAAGCTAAGAGAAGAGCTTATGGTGACGAGTGACAAGACAATTGACGCACGAGGTGCAAATGTGCACATTGAGGATGGTGCACAAATTACACTACAGTTTGTGAAGAATGTGATCATCCATAATCTCCACATTCATGATACCAAGCCAGGAAATGGTGGCATGATTAGGGACTCGGTGAATCACTATGGACAACGCACACGCAGTGATGGTGACGGTATATCAATGTATGGAGCCTCAAATGTTTGGATAGACCATGTCTCCGGGTCAAATTGCGCAGATGGACTCGTTGATGCCATCCAGGGGTCTACAGCCATCACCATATCCAACTGTCACTTCACCAATCATAATGATGTGATGTTATTTGGTTCAAGTGATAGCAACTCTCAGGATGAAGTGATGCAGATAACTCTGGCTTTCAACCATTTCGGTCAGGGGTTAATTCAGCGTATGCCAAGGTGCCGATGGGGGTTTTTCCATGTTGTAAACAATGACTACACGCATTGGATTATGTACGCTATTGGTGGAAGCCAACATCCTACCATAATTAGCCAGGGCAACCGGTTCATTGCCCCGTCCAATAATGCCAGTAAAGAGGTGACGAAGAGGACAAGTGGAGCCGAGAATGAGTGGAAATCATGGAATTGGAGATCAGAGAACGATCTTATGATGAATGGAGCATTCTTTGTTGAATCCGGAAGCCCAATCAGGAATCTCCCAAAGGCGGATATGATCAAGGCAAAGCCTGGGACATTTGTGACTAGGCTCACGCGCTTTGCAGGTCCTCTAAAATGCATTAAAGGGAAGCCATGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00040 ko02024 map00040 map02024

Pfam Domains

Protein Families

Pec_lyase_C Pec_lyase_N

Protein Analysis

440

Amino Acids

49.03

Weight (kDa)

9.06

Isoelectric Point (pI)

29.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pec_lyase_N	PF04431	28 - 80	1.5e-19	Pectate lyase, N terminus
Pectate_lyase_4	PF00544	119 - 374	5.3e-25	Pectate lyase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003744)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01270 AT5G15110
fragaria_vesca	FvH4_5g15430 FvH4_6g49230
malus_domestica	MD06G1188600.v1.1 MD09G1047400.v1.1 MD14G1195000.v1.1 MD14G1195200.v1.1 MD17G1047500.v1.1
prunus_persica	Prupe.3G271600_v2.0.a1 Prupe.5G186900_v2.0.a1
pyrus_communis	pycom06g16740 pycom17g04230
rosa_chinensis	RchiOBHm_Chr2g0169091 RchiOBHm_Chr7g0180651
rosa_laevigata	RLG00000005246 RLG00000021833
rosa_multiflora	Rmu_co8469407.1_g000001 Rmu_co8473671.1_g000001 Rmu_ssc0000393.1_g000032
rosa_roxburghii	Rroxscaffold_2G00082470
rosa_rugosa	Rorug02G0536800 Rorug06G0434600
rosa_samantha	Rh2AG608700 Rh2BG621600 Rh2CG590300 Rh2DG632200 Rh7AG036000 Rh7BG035900 Rh7CG037500 Rh7DG036100
rosa_wichuraiana	Rw2G050470 Rw7G002960 Rw7G002970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	781
Acc36I	ACCTGC	1 cut(s)	1274
AccB1I	GGYRCC	1 cut(s)	963
AccB7I	CCANNNNNTGG	1 cut(s)	659
AccI	GTMKAC	1 cut(s)	812
AccII	CGCG	3 cut(s)	101, 218, 1276
AccIII	TCCGGA	1 cut(s)	1199
AciI	CCGC	4 cut(s)	89, 138, 216, 1229
AclWI	GGATC	1 cut(s)	488
AdeI	CACNNNGTG	3 cut(s)	457, 560, 710
AfaI	GTAC	2 cut(s)	251, 1019
AfiI	CCNNNNNNNGG	5 cut(s)	310, 437, 659, 807, 1228
AflIII	ACRYGT	1 cut(s)	204
AgeI	ACCGGT	1 cut(s)	1065
AgsI	TTSAA	5 cut(s)	187, 241, 876, 925, 1196
AjnI	CCWGG	6 cut(s)	264, 436, 652, 804, 1056, 1249
AjuI	GAANNNNNNNTTGG	2 cut(s)	824, 856
AluBI	AGCT	4 cut(s)	8, 331, 511, 523
AluI	AGCT	4 cut(s)	8, 331, 511, 523
Alw21I	GWGCWC	2 cut(s)	573, 589
Alw26I	GTCTC	2 cut(s)	489, 769
Alw44I	GTGCAC	2 cut(s)	569, 585
AlwI	GGATC	1 cut(s)	488
Ama87I	CYCGRG	1 cut(s)	492
Aor13HI	TCCGGA	1 cut(s)	1199
AoxI	GGCC	2 cut(s)	78, 470
ApaLI	GTGCAC	2 cut(s)	569, 585
AsiGI	ACCGGT	1 cut(s)	1065
AspLEI	GCGC	4 cut(s)	103, 344, 782, 1278
AspS9I	GGNCC	4 cut(s)	78, 257, 470, 1286
AsuC2I	CCSGG	1 cut(s)	769
AsuHPI	GGTGA	9 cut(s)	85, 409, 436, 541, 694, 728, 814, 835, 1114
AvaI	CYCGRG	1 cut(s)	492
AvaII	GGWCC	2 cut(s)	257, 1286
BaeGI	GKGCMC	3 cut(s)	360, 573, 589
BanI	GGYRCC	1 cut(s)	963
BauI	CACGAG	1 cut(s)	555
Bbv12I	GWGCWC	2 cut(s)	573, 589
BccI	CCATC	6 cut(s)	575, 707, 779, 809, 827, 963
BcgI	CGANNNNNNTGC	2 cut(s)	313, 347
BciT130I	CCWGG	6 cut(s)	266, 438, 654, 806, 1058, 1251
BclI	TGATCA	2 cut(s)	618, 1236
BcnI	CCSGG	1 cut(s)	769
BcoDI	GTCTC	2 cut(s)	489, 769
BfaI	CTAG	2 cut(s)	1266, 1318
BfmI	CTRYAG	2 cut(s)	599, 813
BfuAI	ACCTGC	1 cut(s)	1274
BisI	GCNGC	2 cut(s)	138, 216
BlsI	GCNGC	2 cut(s)	139, 217
Bme1390I	CCNGG	7 cut(s)	266, 438, 654, 769, 806, 1058, 1251
Bme18I	GGWCC	2 cut(s)	257, 1286
BmeT110I	CYCGRG	1 cut(s)	492
BmgT120I	GGNCC	4 cut(s)	78, 257, 470, 1286
BmiI	GGNNCC	4 cut(s)	258, 739, 965, 1123
BmrFI	CCNGG	7 cut(s)	266, 438, 654, 769, 806, 1058, 1251
BmrI	ACTGGG	2 cut(s)	319, 477
BmsI	GCATC	3 cut(s)	202, 787, 894
BmuI	ACTGGG	2 cut(s)	319, 477
BpmI	CTGGAG	1 cut(s)	313
BpuMI	CCSGG	1 cut(s)	769
BsaJI	CCNNGG	5 cut(s)	437, 805, 959, 1057, 1250
BsaWI	WCCGGW	2 cut(s)	1065, 1199
BsaXI	ACNNNNNCTCC	2 cut(s)	749, 779
Bsc4I	CCNNNNNNNGG	5 cut(s)	310, 437, 659, 807, 1228
Bse118I	RCCGGY	1 cut(s)	1065
Bse1I	ACTGG	4 cut(s)	110, 314, 472, 1092
Bse3DI	GCAATG	2 cut(s)	84, 1071
BseAI	TCCGGA	1 cut(s)	1199
BseBI	CCWGG	6 cut(s)	266, 438, 654, 806, 1058, 1251
BseDI	CCNNGG	5 cut(s)	437, 805, 959, 1057, 1250
BseGI	GGATG	5 cut(s)	586, 621, 801, 901, 1039
BseLI	CCNNNNNNNGG	5 cut(s)	310, 437, 659, 807, 1228
BseMI	GCAATG	2 cut(s)	84, 1071
BseMII	CTCAG	1 cut(s)	905
BseNI	ACTGG	4 cut(s)	110, 314, 472, 1092
BseSI	GKGCMC	3 cut(s)	360, 573, 589
BsgI	GTGCAG	1 cut(s)	150
Bsh1236I	CGCG	3 cut(s)	101, 218, 1276
BshFI	GGCC	2 cut(s)	80, 472
BshNI	GGYRCC	1 cut(s)	963
BshTI	ACCGGT	1 cut(s)	1065
BsiHKAI	GWGCWC	2 cut(s)	573, 589
BsiHKCI	CYCGRG	1 cut(s)	492
BsiSI	CCGG	4 cut(s)	65, 768, 1066, 1200
BslFI	GGGAC	3 cut(s)	454, 689, 1267
BslI	CCNNNNNNNGG	5 cut(s)	310, 437, 659, 807, 1228
BsmAI	GTCTC	2 cut(s)	489, 769
BsmFI	GGGAC	3 cut(s)	454, 689, 1267
BsmI	GAATGC	1 cut(s)	1184
BsnI	GGCC	2 cut(s)	80, 472
BsoBI	CYCGRG	1 cut(s)	492
Bsp1286I	GDGCHC	3 cut(s)	360, 573, 589
Bsp13I	TCCGGA	1 cut(s)	1199
Bsp143I	GATC	6 cut(s)	283, 480, 618, 1154, 1165, 1236
BspACI	CCGC	4 cut(s)	89, 138, 216, 1229
BspANI	GGCC	2 cut(s)	80, 472
BspCNI	CTCAG	1 cut(s)	904
BspEI	TCCGGA	1 cut(s)	1199
BspFNI	CGCG	3 cut(s)	101, 218, 1276
BspHI	TCATGA	1 cut(s)	640
BspLI	GGNNCC	4 cut(s)	258, 739, 965, 1123
BspMI	ACCTGC	1 cut(s)	1274
BspPI	GGATC	1 cut(s)	488
BspQI	GCTCTTC	1 cut(s)	513
BspT107I	GGYRCC	1 cut(s)	963
BsrDI	GCAATG	2 cut(s)	84, 1071
BsrFI	RCCGGY	1 cut(s)	1065
BsrI	ACTGG	4 cut(s)	110, 314, 472, 1092
BssAI	RCCGGY	1 cut(s)	1065
BssECI	CCNNGG	5 cut(s)	437, 805, 959, 1057, 1250
BssMI	GATC	6 cut(s)	283, 480, 618, 1154, 1165, 1236
BssSI	CACGAG	1 cut(s)	555
BssT1I	CCWWGG	1 cut(s)	959
Bst2BI	CACGAG	1 cut(s)	555
Bst2UI	CCWGG	6 cut(s)	266, 438, 654, 806, 1058, 1251
Bst4CI	ACNGT	4 cut(s)	73, 603, 722, 836
Bst6I	CTCTTC	2 cut(s)	513, 1103
BstAPI	GCANNNNNTGC	1 cut(s)	560
BstC8I	GCNNGC	2 cut(s)	135, 333
BstDEI	CTNAG	2 cut(s)	512, 891
BstF5I	GGATG	5 cut(s)	586, 621, 801, 901, 1039
BstFNI	CGCG	3 cut(s)	101, 218, 1276
BstHHI	GCGC	4 cut(s)	103, 344, 782, 1278
BstKTI	GATC	6 cut(s)	286, 483, 621, 1157, 1168, 1239
BstMAI	GTCTC	2 cut(s)	489, 769
BstMBI	GATC	6 cut(s)	283, 480, 618, 1154, 1165, 1236
BstMWI	GCNNNNNNNGC	6 cut(s)	86, 125, 134, 146, 341, 560
BstNI	CCWGG	6 cut(s)	266, 438, 654, 806, 1058, 1251
BstSCI	CCNGG	7 cut(s)	264, 436, 652, 767, 804, 1056, 1249
BstSFI	CTRYAG	2 cut(s)	599, 813
BstSLI	GKGCMC	3 cut(s)	360, 573, 589
BstUI	CGCG	3 cut(s)	101, 218, 1276
BsuRI	GGCC	2 cut(s)	80, 472
BtsCI	GGATG	5 cut(s)	586, 621, 801, 901, 1039
BtsI	GCAGTG	1 cut(s)	715
BtsIMutI	CAGTG	3 cut(s)	103, 174, 715
BveI	ACCTGC	1 cut(s)	1274
Cac8I	GCNNGC	2 cut(s)	135, 333
CciI	TCATGA	1 cut(s)	640
CfoI	GCGC	4 cut(s)	103, 344, 782, 1278
Cfr10I	RCCGGY	1 cut(s)	1065
Cfr13I	GGNCC	4 cut(s)	78, 257, 470, 1286
CseI	GACGC	2 cut(s)	107, 560
Csp6I	GTAC	2 cut(s)	250, 1018
CspAI	ACCGGT	1 cut(s)	1065
CviAII	CATG	9 cut(s)	261, 421, 499, 641, 667, 761, 983, 1143, 1314
CviQI	GTAC	2 cut(s)	250, 1018
DdeI	CTNAG	2 cut(s)	512, 891
DpnI	GATC	6 cut(s)	285, 482, 620, 1156, 1167, 1238
DpnII	GATC	6 cut(s)	283, 480, 618, 1154, 1165, 1236
DraIII	CACNNNGTG	3 cut(s)	457, 560, 710
Eam1104I	CTCTTC	2 cut(s)	513, 1103
EarI	CTCTTC	2 cut(s)	513, 1103
EciI	GGCGGA	1 cut(s)	1244
Eco130I	CCWWGG	1 cut(s)	959
Eco47I	GGWCC	2 cut(s)	257, 1286
Eco88I	CYCGRG	1 cut(s)	492
EcoO109I	RGGNCCY	1 cut(s)	1286
EcoRII	CCWGG	6 cut(s)	264, 436, 652, 804, 1056, 1249
EcoT14I	CCWWGG	1 cut(s)	959
EcoT22I	ATGCAT	1 cut(s)	1301
ErhI	CCWWGG	1 cut(s)	959
FaeI	CATG	9 cut(s)	264, 424, 502, 644, 670, 764, 986, 1146, 1317
FaqI	GGGAC	3 cut(s)	454, 689, 1267
FatI	CATG	9 cut(s)	260, 420, 498, 640, 666, 760, 982, 1142, 1313
FbaI	TGATCA	2 cut(s)	618, 1236
FblI	GTMKAC	1 cut(s)	812
Fnu4HI	GCNGC	2 cut(s)	138, 216
FokI	GGATG	5 cut(s)	593, 608, 788, 908, 1026
Fsp4HI	GCNGC	2 cut(s)	138, 216
FspBI	CTAG	2 cut(s)	1266, 1318
FspI	TGCGCA	1 cut(s)	781
GlaI	GCGC	4 cut(s)	102, 343, 781, 1277
GluI	GCNGC	2 cut(s)	138, 216
GsuI	CTGGAG	1 cut(s)	313
HaeIII	GGCC	2 cut(s)	80, 472
HapII	CCGG	4 cut(s)	65, 768, 1066, 1200
HgaI	GACGC	2 cut(s)	107, 560
HhaI	GCGC	4 cut(s)	103, 344, 782, 1278
Hin1II	CATG	9 cut(s)	264, 424, 502, 644, 670, 764, 986, 1146, 1317
Hin6I	GCGC	4 cut(s)	101, 342, 780, 1276
HinP1I	GCGC	4 cut(s)	101, 342, 780, 1276
HinfI	GANTC	5 cut(s)	677, 685, 789, 1196, 1216
HpaII	CCGG	4 cut(s)	65, 768, 1066, 1200
HphI	GGTGA	9 cut(s)	85, 409, 436, 541, 694, 728, 814, 835, 1114
Hpy166II	GTNNAC	6 cut(s)	199, 427, 571, 587, 813, 991
Hpy188I	TCNGA	2 cut(s)	412, 1159
Hpy188III	TCNNGA	5 cut(s)	494, 641, 893, 1200, 1213
Hpy8I	GTNNAC	6 cut(s)	199, 427, 571, 587, 813, 991
Hpy99I	CGWCG	1 cut(s)	101
HpyAV	CCTTC	1 cut(s)	225
HpyCH4III	ACNGT	4 cut(s)	73, 603, 722, 836
HpyCH4IV	ACGT	1 cut(s)	206
HpyCH4V	TGCA	7 cut(s)	167, 563, 571, 587, 907, 1283, 1299
HpyF10VI	GCNNNNNNNGC	6 cut(s)	86, 125, 134, 146, 341, 560
HpyF3I	CTNAG	2 cut(s)	512, 891
HpySE526I	ACGT	1 cut(s)	206
Hsp92II	CATG	9 cut(s)	264, 424, 502, 644, 670, 764, 986, 1146, 1317
HspAI	GCGC	4 cut(s)	101, 342, 780, 1276
Kpn2I	TCCGGA	1 cut(s)	1199
Ksp22I	TGATCA	2 cut(s)	618, 1236
Kzo9I	GATC	6 cut(s)	283, 480, 618, 1154, 1165, 1236
LguI	GCTCTTC	1 cut(s)	513
LmnI	GCTCC	3 cut(s)	737, 1121, 1181
LweI	GCATC	3 cut(s)	202, 787, 894
MaeI	CTAG	2 cut(s)	1266, 1318
MaeII	ACGT	1 cut(s)	206
MaeIII	GTNAC	9 cut(s)	172, 299, 397, 529, 536, 716, 836, 1102, 1261
MalI	GATC	6 cut(s)	285, 482, 620, 1156, 1167, 1238
MboI	GATC	6 cut(s)	283, 480, 618, 1154, 1165, 1236
MboII	GAAGA	3 cut(s)	530, 622, 1120
MfeI	CAATTG	1 cut(s)	546
MhlI	GDGCHC	3 cut(s)	360, 573, 589
MluCI	AATT	8 cut(s)	10, 60, 546, 591, 775, 945, 1050, 1147
MlyI	GAGTC	2 cut(s)	671, 783
MmeI	TCCRAC	1 cut(s)	855
MnlI	CCTC	8 cut(s)	69, 365, 551, 571, 751, 1093, 1104, 1299
Mph1103I	ATGCAT	1 cut(s)	1301
MroI	TCCGGA	1 cut(s)	1199
MseI	TTAA	2 cut(s)	944, 1302
MslI	CAYNNNNRTG	2 cut(s)	639, 855
MspI	CCGG	4 cut(s)	65, 768, 1066, 1200
MspR9I	CCNGG	7 cut(s)	266, 438, 654, 769, 806, 1058, 1251
MunI	CAATTG	1 cut(s)	546
Mva1269I	GAATGC	1 cut(s)	1184
MvaI	CCWGG	6 cut(s)	266, 438, 654, 806, 1058, 1251
MvnI	CGCG	3 cut(s)	101, 218, 1276
MwoI	GCNNNNNNNGC	6 cut(s)	86, 125, 134, 146, 341, 560
NciI	CCSGG	1 cut(s)	769
NdeII	GATC	6 cut(s)	283, 480, 618, 1154, 1165, 1236
NlaIII	CATG	9 cut(s)	264, 424, 502, 644, 670, 764, 986, 1146, 1317
NlaIV	GGNNCC	4 cut(s)	258, 739, 965, 1123
NmeAIII	GCCGAG	1 cut(s)	1150
NmuCI	GTSAC	9 cut(s)	172, 299, 397, 529, 536, 716, 836, 1102, 1261
NsbI	TGCGCA	1 cut(s)	781
NsiI	ATGCAT	1 cut(s)	1301
PaeR7I	CTCGAG	1 cut(s)	492
PagI	TCATGA	1 cut(s)	640
PciSI	GCTCTTC	1 cut(s)	513
PctI	GAATGC	1 cut(s)	1184
PfeI	GAWTC	3 cut(s)	685, 1196, 1216
PflFI	GACNNNGTC	1 cut(s)	761
PflMI	CCANNNNNTGG	1 cut(s)	659
PinAI	ACCGGT	1 cut(s)	1065
PkrI	GCNGC	2 cut(s)	139, 217
PleI	GAGTC	2 cut(s)	671, 783
PpsI	GAGTC	2 cut(s)	671, 783
PpuMI	RGGWCCY	1 cut(s)	1286
Psp5II	RGGWCCY	1 cut(s)	1286
Psp6I	CCWGG	6 cut(s)	264, 436, 652, 804, 1056, 1249
PspGI	CCWGG	6 cut(s)	264, 436, 652, 804, 1056, 1249
PspN4I	GGNNCC	4 cut(s)	258, 739, 965, 1123
PspPI	GGNCC	4 cut(s)	78, 257, 470, 1286
PspPPI	RGGWCCY	1 cut(s)	1286
PsyI	GACNNNGTC	1 cut(s)	761
RsaI	GTAC	2 cut(s)	251, 1019
RsaNI	GTAC	2 cut(s)	250, 1018
RseI	CAYNNNNRTG	2 cut(s)	639, 855
SapI	GCTCTTC	1 cut(s)	513
SaqAI	TTAA	2 cut(s)	944, 1302
SatI	GCNGC	2 cut(s)	138, 216
Sau3AI	GATC	6 cut(s)	283, 480, 618, 1154, 1165, 1236
Sau96I	GGNCC	4 cut(s)	78, 257, 470, 1286
SchI	GAGTC	2 cut(s)	671, 783
ScrFI	CCNGG	7 cut(s)	266, 438, 654, 769, 806, 1058, 1251
SduI	GDGCHC	3 cut(s)	360, 573, 589
SfaNI	GCATC	3 cut(s)	202, 787, 894
SfcI	CTRYAG	2 cut(s)	599, 813
Sfr274I	CTCGAG	1 cut(s)	492
SinI	GGWCC	2 cut(s)	257, 1286
SlaI	CTCGAG	1 cut(s)	492
SmiMI	CAYNNNNRTG	2 cut(s)	639, 855
SmlI	CTYRAG	1 cut(s)	492
SmoI	CTYRAG	1 cut(s)	492
Sse9I	AATT	8 cut(s)	10, 60, 546, 591, 775, 945, 1050, 1147
SsiI	CCGC	4 cut(s)	89, 138, 216, 1229
SspMI	CTAG	2 cut(s)	1266, 1318
StyD4I	CCNGG	7 cut(s)	264, 436, 652, 767, 804, 1056, 1249
StyI	CCWWGG	1 cut(s)	959
TaaI	ACNGT	4 cut(s)	73, 603, 722, 836
TaiI	ACGT	1 cut(s)	209
TaqI	TCGA	3 cut(s)	96, 115, 493
TaqII	GACCGA	1 cut(s)	923
TasI	AATT	8 cut(s)	10, 60, 546, 591, 775, 945, 1050, 1147
TatI	WGTACW	1 cut(s)	249
TauI	GCSGC	2 cut(s)	140, 218
TfiI	GAWTC	3 cut(s)	685, 1196, 1216
Tru1I	TTAA	2 cut(s)	944, 1302
Tru9I	TTAA	2 cut(s)	944, 1302
TscAI	CASTG	3 cut(s)	110, 181, 715
TseFI	GTSAC	9 cut(s)	172, 299, 397, 529, 536, 716, 836, 1102, 1261
Tsp45I	GTSAC	9 cut(s)	172, 299, 397, 529, 536, 716, 836, 1102, 1261
TspDTI	ATGAA	4 cut(s)	629, 912, 1060, 1190
TspRI	CASTG	3 cut(s)	110, 181, 715
Tth111I	GACNNNGTC	1 cut(s)	761
Van91I	CCANNNNNTGG	1 cut(s)	659
VneI	GTGCAC	2 cut(s)	569, 585
VpaK11BI	GGWCC	2 cut(s)	257, 1286
XcmI	CCANNNNNNNNNTGG	1 cut(s)	966
XhoI	CTCGAG	1 cut(s)	492
XmiI	GTMKAC	1 cut(s)	812
XspI	CTAG	2 cut(s)	1266, 1318
Zsp2I	ATGCAT	1 cut(s)	1301

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.