Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0003744

Physical Location & Seq

Genomic Coordinates Forward (+)

3107182 .. 3109599

Gene Structure 2418 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1047400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1380 bp

ATGGTTCTTGGACCAATGGCATCGAAGCTCAATTTGGTCCTCTTTATGATTTCTTTCTGCATGCTGGTTCCAAGCTTGAAGGCTGAGACAGCAAATGACACCCTCAACATTGCTAACTTCGATGCCGTATGGCAGGAACGAGCCATGAAAGCTCAGAAGGCAGCCCTCAAGGCCTACCAACCCAACCCGGAACAAGTCACCGAGGACTTCAACGAAAGCGTTGAACAGACCGTCGTTGAGGAGGACCTTGAAGATGATCAGTCTGCCGGTAACAGCACAAGGAGGAATCTGAGAGGAAAACATTACAAGGGCCCTTGCAAGGCCACAAACCCGATCGACCGCTGCTGGCGGTGCCGGGCCAACTGGGCTAACAACCGCAAGAAGCTGGCCAGCTGCGTGAAAGGCTTTGGCCGCAAGACGCGCGGAGGCAAGAAAGGGGCTTACTACATTGTCACCGACTCTTCGGACGACAATGTCCATGACCCTAAGCCCGGAACCCTACGGCACGCCGTGATCCAAACGCAACCGTTGTGGATCATATTTGCACGCAGCATGGTCATCAGGCTGAGCCAAGAGCTCATAGTAACGAGCCACAAGACAATTGACGCACGAGGGGCAAATGTGCACATTGCTCATGGCGCAGGGATCACACTCCAGTTCGTTCAGAACGTGATTATTCATGGCCTCCGCATCCACGACACCGTACCAGGCAGCGGTGGTATGATCAGGGACTCCCTGAGTCACATCGGGATTCGCACACAGAGTGACGGAGATGGTATCTCCATTTACGGGTCGTCTAACATTTGGCTCGACCATCTTTCTATGTGGAATTGCGCGGATGGGCTCATCGACGCCATTCAAGGGTCTACTGCTATTACCATATCCAACTGCCACTTCACCCATCACAACGACGTGATGCTTTTCGGGGCTAGCGATGCGTTTGCAGGGGATGCAATCATGCAAATAACGGTTGCGTTTAACCATTTCGGCAAGGGATTGATACAGAGAATGCCAAGATGCAGATGGGGATTTGTCCATGTCGTGAACAATGACTACACACATTGGATCATGTATGCCATCGGAGGAAGCTCTCACCCCACAATTATCAGCCAGGGCAACCGATTCATCGCCCCACCAAACCAGGCTGCAAAAGAAGTGACGAAGAGAGACTATGCACCGGAGAGCGTGTGGAAGAGCTGGCAATGGAGATCAGAAGGAGATCTTATGATGAATGGAGCATTCTTTGTTCAGTCTGGAGTGCCGAACAAGAAGCATGGATTTGGCAAGTTAGACATGATCAAAGCAAAACCAGGCACATTCGTCAGAAGGCTCACACGCTTTGCAGGTTCTCTCAACTGCAAGGTTGGCAAGAAGTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00040 ko02024 map00040 map02024

Pfam Domains

Protein Families

Pec_lyase_C Pec_lyase_N

Protein Analysis

460

Amino Acids

50.95

Weight (kDa)

9.38

Isoelectric Point (pI)

35.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pec_lyase_N	PF04431	36 - 97	2.3e-19	Pectate lyase, N terminus
Pectate_lyase_4	PF00544	193 - 389	2.1e-19	Pectate lyase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003744)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01270 AT5G15110
fragaria_vesca	FvH4_5g15430 FvH4_6g49230
malus_domestica	MD06G1188600.v1.1 MD09G1047400.v1.1 MD14G1195000.v1.1 MD14G1195200.v1.1 MD17G1047500.v1.1
prunus_persica	Prupe.3G271600_v2.0.a1 Prupe.5G186900_v2.0.a1
pyrus_communis	pycom06g16740 pycom17g04230
rosa_chinensis	RchiOBHm_Chr2g0169091 RchiOBHm_Chr7g0180651
rosa_laevigata	RLG00000005246 RLG00000021833
rosa_multiflora	Rmu_co8469407.1_g000001 Rmu_co8473671.1_g000001 Rmu_ssc0000393.1_g000032
rosa_roxburghii	Rroxscaffold_2G00082470
rosa_rugosa	Rorug02G0536800 Rorug06G0434600
rosa_samantha	Rh2AG608700 Rh2BG621600 Rh2CG590300 Rh2DG632200 Rh7AG036000 Rh7BG035900 Rh7CG037500 Rh7DG036100
rosa_wichuraiana	Rw2G050470 Rw7G002960 Rw7G002970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	473
Acc36I	ACCTGC	1 cut(s)	1334
AccB1I	GGYRCC	1 cut(s)	351
AccI	GTMKAC	1 cut(s)	866
AccII	CGCG	3 cut(s)	421, 423, 836
AciI	CCGC	8 cut(s)	340, 349, 376, 412, 423, 688, 714, 836
AclWI	GGATC	4 cut(s)	508, 542, 653, 1073
AcoI	YGGCCR	2 cut(s)	387, 409
AcyI	GRCGYC	1 cut(s)	852
AdeI	CACNNNGTG	2 cut(s)	511, 764
AfaI	GTAC	1 cut(s)	705
AfiI	CCNNNNNNNGG	5 cut(s)	319, 491, 713, 789, 861
AgsI	TTSAA	5 cut(s)	79, 211, 224, 251, 860
AjiI	CACGTC	1 cut(s)	913
AjnI	CCWGG	4 cut(s)	706, 1110, 1140, 1309
AjuI	GAANNNNNNNTTGG	4 cut(s)	17, 49, 878, 910
AluBI	AGCT	8 cut(s)	28, 75, 152, 385, 393, 577, 1089, 1197
AluI	AGCT	8 cut(s)	28, 75, 152, 385, 393, 577, 1089, 1197
Alw21I	GWGCWC	2 cut(s)	579, 627
Alw26I	GTCTC	2 cut(s)	80, 1161
Alw44I	GTGCAC	1 cut(s)	623
AlwI	GGATC	4 cut(s)	508, 542, 653, 1073
AoxI	GGCC	7 cut(s)	171, 310, 321, 357, 387, 409, 682
ApaI	GGGCCC	1 cut(s)	314
ApaLI	GTGCAC	1 cut(s)	623
ApeKI	GCWGC	6 cut(s)	161, 342, 393, 549, 711, 1145
AspLEI	GCGC	3 cut(s)	423, 641, 836
AspS9I	GGNCC	6 cut(s)	11, 37, 244, 310, 311, 357
AsuC2I	CCSGG	3 cut(s)	188, 356, 492
AsuHPI	GGTGA	4 cut(s)	190, 445, 889, 1085
AsuNHI	GCTAGC	1 cut(s)	929
AvaII	GGWCC	3 cut(s)	11, 37, 244
BaeGI	GKGCMC	2 cut(s)	314, 627
BalI	TGGCCA	1 cut(s)	389
BanI	GGYRCC	1 cut(s)	351
BanII	GRGCYC	3 cut(s)	314, 579, 846
BauI	CACGAG	1 cut(s)	609
Bbv12I	GWGCWC	2 cut(s)	579, 627
BbvI	GCAGC	6 cut(s)	173, 329, 380, 561, 723, 1132
BccI	CCATC	6 cut(s)	767, 822, 833, 909, 1017, 1085
BceAI	ACGGC	3 cut(s)	110, 494, 518
BcgI	CGANNNNNNTGC	2 cut(s)	923, 957
BciT130I	CCWGG	4 cut(s)	708, 1112, 1142, 1311
BclI	TGATCA	3 cut(s)	256, 723, 1296
BcnI	CCSGG	3 cut(s)	188, 356, 492
BcoDI	GTCTC	2 cut(s)	80, 1161
BfaI	CTAG	2 cut(s)	930, 1378
BfuAI	ACCTGC	1 cut(s)	1334
BglI	GCCNNNNNGGC	2 cut(s)	170, 365
BglII	AGATCT	1 cut(s)	1219
BisI	GCNGC	7 cut(s)	162, 343, 394, 412, 550, 712, 1146
BlpI	GCTNAGC	1 cut(s)	566
BlsI	GCNGC	7 cut(s)	163, 344, 395, 413, 551, 713, 1147
Bme1390I	CCNGG	7 cut(s)	188, 356, 492, 708, 1112, 1142, 1311
Bme18I	GGWCC	3 cut(s)	11, 37, 244
BmgBI	CACGTC	1 cut(s)	913
BmgT120I	GGNCC	6 cut(s)	11, 37, 244, 310, 311, 357
BmiI	GGNNCC	4 cut(s)	69, 312, 353, 496
BmrFI	CCNGG	7 cut(s)	188, 356, 492, 708, 1112, 1142, 1311
BmrI	ACTGGG	1 cut(s)	373
BmsI	GCATC	7 cut(s)	29, 112, 699, 906, 925, 940, 1007
BmtI	GCTAGC	1 cut(s)	933
BmuI	ACTGGG	1 cut(s)	373
BpmI	CTGGAG	2 cut(s)	638, 1275
Bpu10I	CCTNAGC	1 cut(s)	486
Bpu1102I	GCTNAGC	1 cut(s)	566
BpuEI	CTTGAG	1 cut(s)	152
BpuMI	CCSGG	3 cut(s)	188, 356, 492
BsaHI	GRCGYC	1 cut(s)	852
BsaJI	CCNNGG	2 cut(s)	201, 1111
BsaWI	WCCGGW	1 cut(s)	1177
BsaXI	ACNNNNNCTCC	2 cut(s)	1172, 1202
Bsc4I	CCNNNNNNNGG	5 cut(s)	319, 491, 713, 789, 861
Bse118I	RCCGGY	1 cut(s)	266
Bse1I	ACTGG	2 cut(s)	368, 655
Bse3DI	GCAATG	3 cut(s)	108, 627, 1208
BseBI	CCWGG	4 cut(s)	708, 1112, 1142, 1311
BseDI	CCNNGG	2 cut(s)	201, 1111
BseGI	GGATG	3 cut(s)	690, 844, 955
BseLI	CCNNNNNNNGG	5 cut(s)	319, 491, 713, 789, 861
BseMI	GCAATG	3 cut(s)	108, 627, 1208
BseMII	CTCAG	5 cut(s)	75, 167, 281, 557, 728
BseNI	ACTGG	2 cut(s)	368, 655
BseRI	GAGGAG	1 cut(s)	254
BseSI	GKGCMC	2 cut(s)	314, 627
BseXI	GCAGC	6 cut(s)	173, 329, 380, 561, 723, 1132
Bsh1236I	CGCG	3 cut(s)	421, 423, 836
Bsh1285I	CGRYCG	2 cut(s)	336, 340
BshFI	GGCC	7 cut(s)	173, 312, 323, 359, 389, 411, 684
BshNI	GGYRCC	1 cut(s)	351
BsiEI	CGRYCG	2 cut(s)	336, 340
BsiHKAI	GWGCWC	2 cut(s)	579, 627
BsiSI	CCGG	5 cut(s)	188, 267, 355, 492, 1178
BslFI	GGGAC	1 cut(s)	743
BslI	CCNNNNNNNGG	5 cut(s)	319, 491, 713, 789, 861
BsmAI	GTCTC	2 cut(s)	80, 1161
BsmFI	GGGAC	1 cut(s)	743
BsmI	GAATGC	2 cut(s)	1014, 1238
BsnI	GGCC	7 cut(s)	173, 312, 323, 359, 389, 411, 684
Bsp120I	GGGCCC	1 cut(s)	310
Bsp1286I	GDGCHC	4 cut(s)	314, 579, 627, 846
Bsp1720I	GCTNAGC	1 cut(s)	566
BspACI	CCGC	8 cut(s)	340, 349, 376, 412, 423, 688, 714, 836
BspANI	GGCC	7 cut(s)	173, 312, 323, 359, 389, 411, 684
BspCNI	CTCAG	5 cut(s)	76, 166, 282, 558, 729
BspFNI	CGCG	3 cut(s)	421, 423, 836
BspLI	GGNNCC	4 cut(s)	69, 312, 353, 496
BspMI	ACCTGC	1 cut(s)	1334
BspOI	GCTAGC	1 cut(s)	933
BspPI	GGATC	4 cut(s)	508, 542, 653, 1073
BspQI	GCTCTTC	1 cut(s)	1187
BspT107I	GGYRCC	1 cut(s)	351
BsrDI	GCAATG	3 cut(s)	108, 627, 1208
BsrFI	RCCGGY	1 cut(s)	266
BsrI	ACTGG	2 cut(s)	368, 655
BssAI	RCCGGY	1 cut(s)	266
BssECI	CCNNGG	2 cut(s)	201, 1111
BssNI	GRCGYC	1 cut(s)	852
BssSI	CACGAG	1 cut(s)	609
Bst2BI	CACGAG	1 cut(s)	609
Bst2UI	CCWGG	4 cut(s)	708, 1112, 1142, 1311
Bst4CI	ACNGT	4 cut(s)	232, 528, 703, 970
Bst6I	CTCTTC	3 cut(s)	466, 1157, 1187
BstACI	GRCGYC	1 cut(s)	852
BstAPI	GCANNNNNTGC	2 cut(s)	950, 1374
BstC8I	GCNNGC	8 cut(s)	62, 347, 387, 391, 507, 547, 931, 1199
BstDEI	CTNAG	6 cut(s)	84, 153, 290, 486, 566, 737
BstF5I	GGATG	3 cut(s)	690, 844, 955
BstFNI	CGCG	3 cut(s)	421, 423, 836
BstHHI	GCGC	3 cut(s)	423, 641, 836
BstMAI	GTCTC	2 cut(s)	80, 1161
BstMCI	CGRYCG	2 cut(s)	336, 340
BstNI	CCWGG	4 cut(s)	708, 1112, 1142, 1311
BstNSI	RCATGY	1 cut(s)	64
BstSCI	CCNGG	7 cut(s)	186, 354, 490, 706, 1110, 1140, 1309
BstSLI	GKGCMC	2 cut(s)	314, 627
BstUI	CGCG	3 cut(s)	421, 423, 836
BstV1I	GCAGC	6 cut(s)	173, 329, 380, 561, 723, 1132
BstX2I	RGATCY	1 cut(s)	1219
BstYI	RGATCY	1 cut(s)	1219
BsuRI	GGCC	7 cut(s)	173, 312, 323, 359, 389, 411, 684
BtgZI	GCGATG	2 cut(s)	948, 1111
BtrI	CACGTC	1 cut(s)	913
BtsCI	GGATG	3 cut(s)	690, 844, 955
BveI	ACCTGC	1 cut(s)	1334
Cac8I	GCNNGC	8 cut(s)	62, 347, 387, 391, 507, 547, 931, 1199
CfoI	GCGC	3 cut(s)	423, 641, 836
Cfr10I	RCCGGY	1 cut(s)	266
Cfr13I	GGNCC	6 cut(s)	11, 37, 244, 310, 311, 357
CseI	GACGC	3 cut(s)	427, 614, 860
Csp6I	GTAC	1 cut(s)	704
CspCI	CAANNNNNGTGG	2 cut(s)	512, 547
CviQI	GTAC	1 cut(s)	704
DdeI	CTNAG	6 cut(s)	84, 153, 290, 486, 566, 737
DraIII	CACNNNGTG	2 cut(s)	511, 764
DrdI	GACNNNNNNGTC	1 cut(s)	473
DseDI	GACNNNNNNGTC	1 cut(s)	473
EaeI	YGGCCR	2 cut(s)	387, 409
Eam1104I	CTCTTC	3 cut(s)	466, 1157, 1187
EarI	CTCTTC	3 cut(s)	466, 1157, 1187
Ecl136II	GAGCTC	1 cut(s)	577
Eco147I	AGGCCT	1 cut(s)	173
Eco24I	GRGCYC	3 cut(s)	314, 579, 846
Eco47I	GGWCC	3 cut(s)	11, 37, 244
Eco53kI	GAGCTC	1 cut(s)	577
EcoICRI	GAGCTC	1 cut(s)	577
EcoO109I	RGGNCCY	3 cut(s)	244, 310, 311
EcoRII	CCWGG	4 cut(s)	706, 1110, 1140, 1309
EcoT38I	GRGCYC	3 cut(s)	314, 579, 846
FalI	AAGNNNNNCTT	2 cut(s)	1206, 1238
FaqI	GGGAC	1 cut(s)	743
FbaI	TGATCA	3 cut(s)	256, 723, 1296
FblI	GTMKAC	1 cut(s)	866
Fnu4HI	GCNGC	7 cut(s)	162, 343, 394, 412, 550, 712, 1146
FokI	GGATG	3 cut(s)	677, 851, 962
FriOI	GRGCYC	3 cut(s)	314, 579, 846
Fsp4HI	GCNGC	7 cut(s)	162, 343, 394, 412, 550, 712, 1146
FspBI	CTAG	2 cut(s)	930, 1378
GlaI	GCGC	3 cut(s)	422, 640, 835
GluI	GCNGC	7 cut(s)	162, 343, 394, 412, 550, 712, 1146
GsuI	CTGGAG	2 cut(s)	638, 1275
HaeIII	GGCC	7 cut(s)	173, 312, 323, 359, 389, 411, 684
HapII	CCGG	5 cut(s)	188, 267, 355, 492, 1178
HgaI	GACGC	3 cut(s)	427, 614, 860
HhaI	GCGC	3 cut(s)	423, 641, 836
Hin1I	GRCGYC	1 cut(s)	852
Hin6I	GCGC	3 cut(s)	421, 639, 834
HinP1I	GCGC	3 cut(s)	421, 639, 834
HindIII	AAGCTT	1 cut(s)	73
HinfI	GANTC	6 cut(s)	286, 458, 731, 739, 751, 1122
HpaII	CCGG	5 cut(s)	188, 267, 355, 492, 1178
HphI	GGTGA	4 cut(s)	190, 445, 889, 1085
Hpy166II	GTNNAC	3 cut(s)	625, 867, 1045
Hpy188I	TCNGA	7 cut(s)	156, 291, 466, 666, 1082, 1213, 1325
Hpy188III	TCNNGA	3 cut(s)	748, 1042, 1254
Hpy8I	GTNNAC	3 cut(s)	625, 867, 1045
Hpy99I	CGWCG	3 cut(s)	236, 854, 914
HpyAV	CCTTC	4 cut(s)	73, 151, 1208, 1320
HpyCH4III	ACNGT	4 cut(s)	232, 528, 703, 970
HpyCH4IV	ACGT	2 cut(s)	669, 912
HpyF3I	CTNAG	6 cut(s)	84, 153, 290, 486, 566, 737
HpySE526I	ACGT	2 cut(s)	669, 912
Hsp92I	GRCGYC	1 cut(s)	852
HspAI	GCGC	3 cut(s)	421, 639, 834
Ksp22I	TGATCA	3 cut(s)	256, 723, 1296
LguI	GCTCTTC	1 cut(s)	1187
LmnI	GCTCC	1 cut(s)	1235
Lsp1109I	GCAGC	6 cut(s)	173, 329, 380, 561, 723, 1132
LweI	GCATC	7 cut(s)	29, 112, 699, 906, 925, 940, 1007
MaeI	CTAG	2 cut(s)	930, 1378
MaeII	ACGT	2 cut(s)	669, 912
MaeIII	GTNAC	7 cut(s)	196, 269, 451, 583, 740, 764, 1156
MboII	GAAGA	4 cut(s)	263, 453, 1174, 1204
MfeI	CAATTG	1 cut(s)	600
MflI	RGATCY	1 cut(s)	1219
MhlI	GDGCHC	4 cut(s)	314, 579, 627, 846
MlsI	TGGCCA	1 cut(s)	389
MluCI	AATT	4 cut(s)	31, 600, 829, 1101
MluNI	TGGCCA	1 cut(s)	389
MlyI	GAGTC	3 cut(s)	452, 725, 748
MmeI	TCCRAC	1 cut(s)	909
Mox20I	TGGCCA	1 cut(s)	389
MscI	TGGCCA	1 cut(s)	389
MseI	TTAA	1 cut(s)	978
Msp20I	TGGCCA	1 cut(s)	389
MspA1I	CMGCKG	3 cut(s)	342, 393, 714
MspI	CCGG	5 cut(s)	188, 267, 355, 492, 1178
MspR9I	CCNGG	7 cut(s)	188, 356, 492, 708, 1112, 1142, 1311
MunI	CAATTG	1 cut(s)	600
Mva1269I	GAATGC	2 cut(s)	1014, 1238
MvaI	CCWGG	4 cut(s)	708, 1112, 1142, 1311
MvnI	CGCG	3 cut(s)	421, 423, 836
NciI	CCSGG	3 cut(s)	188, 356, 492
NheI	GCTAGC	1 cut(s)	929
NlaIV	GGNNCC	4 cut(s)	69, 312, 353, 496
NmuCI	GTSAC	5 cut(s)	196, 451, 740, 764, 1156
NspI	RCATGY	1 cut(s)	64
PaeI	GCATGC	1 cut(s)	64
PceI	AGGCCT	1 cut(s)	173
PciSI	GCTCTTC	1 cut(s)	1187
PcsI	WCGNNNNNNNCGW	2 cut(s)	666, 930
PctI	GAATGC	2 cut(s)	1014, 1238
PfeI	GAWTC	3 cut(s)	286, 751, 1122
PflFI	GACNNNGTC	1 cut(s)	473
PkrI	GCNGC	7 cut(s)	163, 344, 395, 413, 551, 713, 1147
Ple19I	CGATCG	1 cut(s)	336
PleI	GAGTC	3 cut(s)	452, 725, 747
PpsI	GAGTC	3 cut(s)	452, 725, 747
PpuMI	RGGWCCY	1 cut(s)	244
Psp124BI	GAGCTC	1 cut(s)	579
Psp5II	RGGWCCY	1 cut(s)	244
Psp6I	CCWGG	4 cut(s)	706, 1110, 1140, 1309
PspGI	CCWGG	4 cut(s)	706, 1110, 1140, 1309
PspN4I	GGNNCC	4 cut(s)	69, 312, 353, 496
PspOMI	GGGCCC	1 cut(s)	310
PspPI	GGNCC	6 cut(s)	11, 37, 244, 310, 311, 357
PspPPI	RGGWCCY	1 cut(s)	244
PsrI	GAACNNNNNNTAC	2 cut(s)	1037, 1069
PsuI	RGATCY	1 cut(s)	1219
PsyI	GACNNNGTC	1 cut(s)	473
PvuI	CGATCG	1 cut(s)	336
PvuII	CAGCTG	1 cut(s)	393
RsaI	GTAC	1 cut(s)	705
RsaNI	GTAC	1 cut(s)	704
SacI	GAGCTC	1 cut(s)	579
SapI	GCTCTTC	1 cut(s)	1187
SaqAI	TTAA	1 cut(s)	978
SatI	GCNGC	7 cut(s)	162, 343, 394, 412, 550, 712, 1146
Sau96I	GGNCC	6 cut(s)	11, 37, 244, 310, 311, 357
SchI	GAGTC	3 cut(s)	452, 725, 748
ScrFI	CCNGG	7 cut(s)	188, 356, 492, 708, 1112, 1142, 1311
SduI	GDGCHC	4 cut(s)	314, 579, 627, 846
SfaNI	GCATC	7 cut(s)	29, 112, 699, 906, 925, 940, 1007
SinI	GGWCC	3 cut(s)	11, 37, 244
SmlI	CTYRAG	1 cut(s)	167
SmoI	CTYRAG	1 cut(s)	167
SphI	GCATGC	1 cut(s)	64
Sse9I	AATT	4 cut(s)	31, 600, 829, 1101
SseBI	AGGCCT	1 cut(s)	173
SsiI	CCGC	8 cut(s)	340, 349, 376, 412, 423, 688, 714, 836
SspMI	CTAG	2 cut(s)	930, 1378
SstI	GAGCTC	1 cut(s)	579
StuI	AGGCCT	1 cut(s)	173
StyD4I	CCNGG	7 cut(s)	186, 354, 490, 706, 1110, 1140, 1309
TaaI	ACNGT	4 cut(s)	232, 528, 703, 970
TaiI	ACGT	2 cut(s)	672, 915
TaqI	TCGA	5 cut(s)	23, 120, 336, 810, 849
TasI	AATT	4 cut(s)	31, 600, 829, 1101
TauI	GCSGC	1 cut(s)	414
TfiI	GAWTC	3 cut(s)	286, 751, 1122
Tru1I	TTAA	1 cut(s)	978
Tru9I	TTAA	1 cut(s)	978
TseFI	GTSAC	5 cut(s)	196, 451, 740, 764, 1156
TseI	GCWGC	6 cut(s)	161, 342, 393, 549, 711, 1145
Tsp45I	GTSAC	5 cut(s)	196, 451, 740, 764, 1156
TspDTI	ATGAA	4 cut(s)	161, 668, 1114, 1244
TspGWI	ACGGA	1 cut(s)	783
Tth111I	GACNNNGTC	1 cut(s)	473
VneI	GTGCAC	1 cut(s)	623
VpaK11BI	GGWCC	3 cut(s)	11, 37, 244
XceI	RCATGY	1 cut(s)	64
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1020
XmiI	GTMKAC	1 cut(s)	866
XspI	CTAG	2 cut(s)	930, 1378

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.