Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0003744

Physical Location & Seq

Genomic Coordinates Reverse (-)

66204149 .. 66205763

Gene Structure 1615 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000005246

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

ATGGCAATGGAAGCTGCTCACAAGCTGAAGCTGCTATTTTTATGTTTTCTGATGACAATAACAATTCCGGCATTGAAGGCCAACATCGGGGAATTTGATGAGGAGTGGGAGAGGCGACAGGAAGAAGCCGAAAAGTCTGCGAAAGCGGCCTACAAGCCTGAACCTCTACATATTACAACCCATTTGAACAGACAAGTCCACAAGACATTGGAGGGTCTTAACGACACAAGAAGGAACTTGAGACACCATTATGATGGTCCATGCCAGGTTACAAACCCAATCGACGCTTGCTGGAGATGCGATCCAAATTGGGATCAAAATCGGCAGAAGCTAGCCGATTGTGCTCTTGGCTTTGGACATGAAACAACCGGAGGCAAAGGTGGACGTATCTATACCGTCACTGATCCTAGTGACGATAACGTGCTCGAACCAGAAGAGGGTACATTGCGGTGGGCTGTGATTCAACCTGAACCGCTCTGGATCATATTTAAAGATGACATGAATATAGAACTGAAAGAAGAGCTCATGGTCACTAGTAACAAGACCATTGATGCAAGGGGGTGTAATGTGCACATTCAGGGCGGTGCCCAAATTACTCTGCAGTATGTGCAGAACATCATTATCTGTAACCTTCACGTTCGTGACACCGTTTCCAAGGAAGGTGGCATGGTTAGGGACTCGACGGGTCACTTTGGACTACGTACAGCCAGCGATGGTGATGGAATATCCTTGTTTGGTGCCTCAAACGTTTGGATAGACCATGTTTCTGCCTCAAATTGCGCCGATGGACTCATCGATGTTGTTGCAGGATCTACCGCCATTACCATATCCAACTCTCACTTCACCGATCATAATGATGTGATGCTGTTTGGTTCAAGCGATAGCTACTATAAGGATGAACTTATGCAGATAACTCTAGCTTTCAACCATTTCGGACAGGGATTGATACAGAGGATGCCAAGGTGCAGATTTGGTTTTTTCCATGTTGTGAACAATGACTACACTCACTGGATTATGTATGCCATCGGTGGCAGCAGCCATCCTACCATACTAAGCCAGGGAAATCGCTACATTGCTCCAGAAAATACTGCAGCTAAAGAGATATGTAATAGAGTCAACACAGCAAAGACTGAGTGGAGTAAATGGAGTTGGACATCAGAAGGAGATCTTTTCCTGAATGGAGCGACTTTTACTGAATCTGGAAGCCCGATTCGGAATATCCCAGAAGAGGATATGATAACTGCAAAGCCTGGGACATTCGTAACCACGCTCACAATGTTTGCCGGTCCTCTTAAATGTGATGAAGGCAAGCCATGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00040 ko02024 map00040 map02024

Pfam Domains

Protein Families

Pec_lyase_C Pec_lyase_N

Protein Analysis

440

Amino Acids

49.04

Weight (kDa)

5.5

Isoelectric Point (pI)

39.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pec_lyase_N	PF04431	28 - 80	3.4e-21	Pectate lyase, N terminus
Pectate_lyase_4	PF00544	172 - 374	9.8e-20	Pectate lyase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003744)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01270 AT5G15110
fragaria_vesca	FvH4_5g15430 FvH4_6g49230
malus_domestica	MD06G1188600.v1.1 MD09G1047400.v1.1 MD14G1195000.v1.1 MD14G1195200.v1.1 MD17G1047500.v1.1
prunus_persica	Prupe.3G271600_v2.0.a1 Prupe.5G186900_v2.0.a1
pyrus_communis	pycom06g16740 pycom17g04230
rosa_chinensis	RchiOBHm_Chr2g0169091 RchiOBHm_Chr7g0180651
rosa_laevigata	RLG00000005246 RLG00000021833
rosa_multiflora	Rmu_co8469407.1_g000001 Rmu_co8473671.1_g000001 Rmu_ssc0000393.1_g000032
rosa_roxburghii	Rroxscaffold_2G00082470
rosa_rugosa	Rorug02G0536800 Rorug06G0434600
rosa_samantha	Rh2AG608700 Rh2BG621600 Rh2CG590300 Rh2DG632200 Rh7AG036000 Rh7BG035900 Rh7CG037500 Rh7DG036100
rosa_wichuraiana	Rw2G050470 Rw7G002960 Rw7G002970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	584, 737
AccBSI	CCGCTC	1 cut(s)	475
AciI	CCGC	5 cut(s)	146, 448, 473, 582, 816
AclI	AACGTT	1 cut(s)	747
AclWI	GGATC	5 cut(s)	296, 321, 398, 488, 817
AcsI	RAATTY	1 cut(s)	92
AcuI	CTGAAG	1 cut(s)	47
AfaI	GTAC	2 cut(s)	442, 703
AfiI	CCNNNNNNNGG	3 cut(s)	87, 437, 1228
AgsI	TTSAA	5 cut(s)	76, 187, 464, 876, 925
AhlI	ACTAGT	1 cut(s)	533
AjnI	CCWGG	3 cut(s)	264, 1056, 1249
AjuI	GAANNNNNNNTTGG	2 cut(s)	824, 856
AleI	CACNNNNGTG	1 cut(s)	639
AluBI	AGCT	8 cut(s)	14, 25, 31, 331, 523, 885, 920, 1094
AluI	AGCT	8 cut(s)	14, 25, 31, 331, 523, 885, 920, 1094
Alw21I	GWGCWC	4 cut(s)	346, 426, 525, 573
Alw26I	GTCTC	1 cut(s)	235
Alw44I	GTGCAC	1 cut(s)	569
AlwI	GGATC	5 cut(s)	296, 321, 398, 488, 817
AoxI	GGCC	2 cut(s)	78, 147
ApaLI	GTGCAC	1 cut(s)	569
ApeKI	GCWGC	5 cut(s)	14, 31, 1032, 1035, 1091
ApoI	RAATTY	1 cut(s)	92
AspLEI	GCGC	1 cut(s)	782
AspS9I	GGNCC	2 cut(s)	257, 1286
AsuHPI	GGTGA	2 cut(s)	728, 835
AsuNHI	GCTAGC	1 cut(s)	331
AvaII	GGWCC	2 cut(s)	257, 1286
BaeGI	GKGCMC	2 cut(s)	573, 589
BanI	GGYRCC	2 cut(s)	584, 737
BanII	GRGCYC	1 cut(s)	525
Bbv12I	GWGCWC	4 cut(s)	346, 426, 525, 573
BbvI	GCAGC	4 cut(s)	18, 1044, 1047, 1103
BccI	CCATC	6 cut(s)	248, 707, 713, 779, 1031, 1047
BcgI	CGANNNNNNTGC	4 cut(s)	119, 153, 785, 819
BciT130I	CCWGG	3 cut(s)	266, 1058, 1251
BcoDI	GTCTC	1 cut(s)	235
BcuI	ACTAGT	1 cut(s)	533
BfaI	CTAG	4 cut(s)	332, 408, 534, 917
BfmI	CTRYAG	2 cut(s)	599, 1089
BglII	AGATCT	1 cut(s)	1165
BisI	GCNGC	6 cut(s)	15, 32, 147, 1033, 1036, 1092
BlsI	GCNGC	6 cut(s)	16, 33, 148, 1034, 1037, 1093
Bme1390I	CCNGG	3 cut(s)	266, 1058, 1251
Bme18I	GGWCC	2 cut(s)	257, 1286
BmgT120I	GGNCC	2 cut(s)	257, 1286
BmiI	GGNNCC	2 cut(s)	586, 739
BmrFI	CCNGG	3 cut(s)	266, 1058, 1251
BmsI	GCATC	4 cut(s)	287, 541, 852, 945
BmtI	GCTAGC	1 cut(s)	335
BpmI	CTGGAG	2 cut(s)	313, 1062
BpuEI	CTTGAG	1 cut(s)	259
Bsa29I	ATCGAT	1 cut(s)	795
BsaAI	YACGTR	1 cut(s)	701
BsaBI	GATNNNNATC	1 cut(s)	318
BsaJI	CCNNGG	4 cut(s)	654, 959, 1057, 1250
BsaWI	WCCGGW	1 cut(s)	368
Bsc4I	CCNNNNNNNGG	3 cut(s)	87, 437, 1228
Bse118I	RCCGGY	1 cut(s)	1283
Bse1I	ACTGG	1 cut(s)	1013
Bse3DI	GCAATG	3 cut(s)	12, 443, 1071
Bse8I	GATNNNNATC	1 cut(s)	318
BseBI	CCWGG	3 cut(s)	266, 1058, 1251
BseCI	ATCGAT	1 cut(s)	795
BseDI	CCNNGG	4 cut(s)	654, 959, 1057, 1250
BseGI	GGATG	3 cut(s)	901, 960, 1039
BseJI	GATNNNNATC	1 cut(s)	318
BseLI	CCNNNNNNNGG	3 cut(s)	87, 437, 1228
BseMI	GCAATG	3 cut(s)	12, 443, 1071
BseMII	CTCAG	1 cut(s)	1122
BseNI	ACTGG	1 cut(s)	1013
BseRI	GAGGAG	1 cut(s)	116
BseSI	GKGCMC	2 cut(s)	573, 589
BseXI	GCAGC	4 cut(s)	18, 1044, 1047, 1103
BsgI	GTGCAG	2 cut(s)	629, 985
BshFI	GGCC	2 cut(s)	80, 149
BshNI	GGYRCC	2 cut(s)	584, 737
BshVI	ATCGAT	1 cut(s)	795
BsiHKAI	GWGCWC	4 cut(s)	346, 426, 525, 573
BsiSI	CCGG	3 cut(s)	68, 369, 1284
BslFI	GGGAC	2 cut(s)	689, 1267
BslI	CCNNNNNNNGG	3 cut(s)	87, 437, 1228
BsmAI	GTCTC	1 cut(s)	235
BsmFI	GGGAC	2 cut(s)	689, 1267
BsnI	GGCC	2 cut(s)	80, 149
Bsp1286I	GDGCHC	5 cut(s)	346, 426, 525, 573, 589
Bsp143I	GATC	7 cut(s)	301, 313, 403, 480, 809, 847, 1165
BspACI	CCGC	5 cut(s)	146, 448, 473, 582, 816
BspANI	GGCC	2 cut(s)	80, 149
BspCNI	CTCAG	1 cut(s)	1123
BspDI	ATCGAT	1 cut(s)	795
BspLI	GGNNCC	2 cut(s)	586, 739
BspMAI	CTGCAG	2 cut(s)	603, 1093
BspOI	GCTAGC	1 cut(s)	335
BspPI	GGATC	5 cut(s)	296, 321, 398, 488, 817
BspQI	GCTCTTC	1 cut(s)	513
BspT107I	GGYRCC	2 cut(s)	584, 737
BsrBI	CCGCTC	1 cut(s)	475
BsrDI	GCAATG	3 cut(s)	12, 443, 1071
BsrFI	RCCGGY	1 cut(s)	1283
BsrI	ACTGG	1 cut(s)	1013
BssAI	RCCGGY	1 cut(s)	1283
BssECI	CCNNGG	4 cut(s)	654, 959, 1057, 1250
BssMI	GATC	7 cut(s)	301, 313, 403, 480, 809, 847, 1165
BssT1I	CCWWGG	2 cut(s)	654, 959
Bst2UI	CCWGG	3 cut(s)	266, 1058, 1251
Bst4CI	ACNGT	2 cut(s)	397, 649
Bst6I	CTCTTC	3 cut(s)	429, 513, 1221
BstAPI	GCANNNNNTGC	1 cut(s)	607
BstBAI	YACGTR	1 cut(s)	701
BstC8I	GCNNGC	4 cut(s)	289, 333, 709, 1310
BstDEI	CTNAG	2 cut(s)	1052, 1131
BstF5I	GGATG	3 cut(s)	901, 960, 1039
BstHHI	GCGC	1 cut(s)	782
BstKTI	GATC	7 cut(s)	304, 316, 406, 483, 812, 850, 1168
BstMAI	GTCTC	1 cut(s)	235
BstMBI	GATC	7 cut(s)	301, 313, 403, 480, 809, 847, 1165
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 31, 77, 146, 297, 341, 607
BstNI	CCWGG	3 cut(s)	266, 1058, 1251
BstSCI	CCNGG	3 cut(s)	264, 1056, 1249
BstSFI	CTRYAG	2 cut(s)	599, 1089
BstSLI	GKGCMC	2 cut(s)	573, 589
BstSNI	TACGTA	1 cut(s)	701
BstV1I	GCAGC	4 cut(s)	18, 1044, 1047, 1103
BstX2I	RGATCY	2 cut(s)	809, 1165
BstXI	CCANNNNNNTGG	1 cut(s)	254
BstYI	RGATCY	2 cut(s)	809, 1165
Bsu15I	ATCGAT	1 cut(s)	795
BsuRI	GGCC	2 cut(s)	80, 149
BsuTUI	ATCGAT	1 cut(s)	795
BtgZI	GCGATG	1 cut(s)	726
BtsCI	GGATG	3 cut(s)	901, 960, 1039
BtsIMutI	CAGTG	2 cut(s)	399, 1006
Cac8I	GCNNGC	4 cut(s)	289, 333, 709, 1310
CfoI	GCGC	1 cut(s)	782
Cfr10I	RCCGGY	1 cut(s)	1283
Cfr13I	GGNCC	2 cut(s)	257, 1286
ClaI	ATCGAT	1 cut(s)	795
CseI	GACGC	1 cut(s)	293
Csp6I	GTAC	2 cut(s)	441, 702
CspCI	CAANNNNNGTGG	2 cut(s)	643, 678
CviAII	CATG	8 cut(s)	261, 359, 499, 526, 667, 761, 983, 1314
CviQI	GTAC	2 cut(s)	441, 702
DdeI	CTNAG	2 cut(s)	1052, 1131
DpnI	GATC	7 cut(s)	303, 315, 405, 482, 811, 849, 1167
DpnII	GATC	7 cut(s)	301, 313, 403, 480, 809, 847, 1165
DraI	TTTAAA	1 cut(s)	490
Eam1104I	CTCTTC	3 cut(s)	429, 513, 1221
EarI	CTCTTC	3 cut(s)	429, 513, 1221
Ecl136II	GAGCTC	1 cut(s)	523
Eco105I	TACGTA	1 cut(s)	701
Eco130I	CCWWGG	2 cut(s)	654, 959
Eco24I	GRGCYC	1 cut(s)	525
Eco47I	GGWCC	2 cut(s)	257, 1286
Eco53kI	GAGCTC	1 cut(s)	523
Eco57I	CTGAAG	1 cut(s)	47
EcoICRI	GAGCTC	1 cut(s)	523
EcoRII	CCWGG	3 cut(s)	264, 1056, 1249
EcoT14I	CCWWGG	2 cut(s)	654, 959
EcoT38I	GRGCYC	1 cut(s)	525
ErhI	CCWWGG	2 cut(s)	654, 959
FaeI	CATG	8 cut(s)	264, 362, 502, 529, 670, 764, 986, 1317
FalI	AAGNNNNNCTT	2 cut(s)	1152, 1184
FaqI	GGGAC	2 cut(s)	689, 1267
FatI	CATG	8 cut(s)	260, 358, 498, 525, 666, 760, 982, 1313
Fnu4HI	GCNGC	6 cut(s)	15, 32, 147, 1033, 1036, 1092
FokI	GGATG	3 cut(s)	908, 967, 1026
FriOI	GRGCYC	1 cut(s)	525
Fsp4HI	GCNGC	6 cut(s)	15, 32, 147, 1033, 1036, 1092
FspBI	CTAG	4 cut(s)	332, 408, 534, 917
GlaI	GCGC	1 cut(s)	781
GluI	GCNGC	6 cut(s)	15, 32, 147, 1033, 1036, 1092
GsuI	CTGGAG	2 cut(s)	313, 1062
HaeIII	GGCC	2 cut(s)	80, 149
HapII	CCGG	3 cut(s)	68, 369, 1284
HgaI	GACGC	1 cut(s)	293
HhaI	GCGC	1 cut(s)	782
Hin1II	CATG	8 cut(s)	264, 362, 502, 529, 670, 764, 986, 1317
Hin6I	GCGC	1 cut(s)	780
HinP1I	GCGC	1 cut(s)	780
HincII	GTYRAC	1 cut(s)	1117
HindII	GTYRAC	1 cut(s)	1117
HinfI	GANTC	6 cut(s)	460, 677, 789, 1113, 1196, 1210
HpaII	CCGG	3 cut(s)	68, 369, 1284
HphI	GGTGA	2 cut(s)	728, 835
Hpy166II	GTNNAC	5 cut(s)	199, 383, 571, 991, 1117
Hpy188I	TCNGA	4 cut(s)	51, 935, 1159, 1215
Hpy188III	TCNNGA	5 cut(s)	478, 641, 1079, 1174, 1200
Hpy8I	GTNNAC	5 cut(s)	199, 383, 571, 991, 1117
Hpy99I	CGWCG	2 cut(s)	287, 685
HpyAV	CCTTC	6 cut(s)	70, 225, 641, 653, 1154, 1298
HpyCH4III	ACNGT	2 cut(s)	397, 649
HpyCH4IV	ACGT	5 cut(s)	385, 420, 636, 700, 747
HpyCH4V	TGCA	9 cut(s)	554, 571, 601, 610, 806, 907, 966, 1091, 1244
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 31, 77, 146, 297, 341, 607
HpyF3I	CTNAG	2 cut(s)	1052, 1131
HpySE526I	ACGT	5 cut(s)	385, 420, 636, 700, 747
Hsp92II	CATG	8 cut(s)	264, 362, 502, 529, 670, 764, 986, 1317
HspAI	GCGC	1 cut(s)	780
Kzo9I	GATC	7 cut(s)	301, 313, 403, 480, 809, 847, 1165
LguI	GCTCTTC	1 cut(s)	513
LmnI	GCTCC	2 cut(s)	1081, 1181
Lsp1109I	GCAGC	4 cut(s)	18, 1044, 1047, 1103
LweI	GCATC	4 cut(s)	287, 541, 852, 945
MaeI	CTAG	4 cut(s)	332, 408, 534, 917
MaeII	ACGT	5 cut(s)	385, 420, 636, 700, 747
MaeIII	GTNAC	9 cut(s)	268, 397, 410, 529, 536, 626, 641, 686, 1261
MalI	GATC	7 cut(s)	303, 315, 405, 482, 811, 849, 1167
MbiI	CCGCTC	1 cut(s)	475
MboI	GATC	7 cut(s)	301, 313, 403, 480, 809, 847, 1165
MboII	GAAGA	4 cut(s)	134, 446, 530, 1238
MflI	RGATCY	2 cut(s)	809, 1165
MhlI	GDGCHC	5 cut(s)	346, 426, 525, 573, 589
MluCI	AATT	5 cut(s)	63, 92, 307, 591, 775
MlyI	GAGTC	3 cut(s)	671, 783, 1122
MmeI	TCCRAC	2 cut(s)	855, 1130
MseI	TTAA	3 cut(s)	219, 489, 1293
MslI	CAYNNNNRTG	5 cut(s)	249, 252, 448, 639, 855
MspI	CCGG	3 cut(s)	68, 369, 1284
MspR9I	CCNGG	3 cut(s)	266, 1058, 1251
MvaI	CCWGG	3 cut(s)	266, 1058, 1251
MwoI	GCNNNNNNNGC	7 cut(s)	11, 31, 77, 146, 297, 341, 607
NdeII	GATC	7 cut(s)	301, 313, 403, 480, 809, 847, 1165
NheI	GCTAGC	1 cut(s)	331
NlaIII	CATG	8 cut(s)	264, 362, 502, 529, 670, 764, 986, 1317
NlaIV	GGNNCC	2 cut(s)	586, 739
NmuCI	GTSAC	5 cut(s)	397, 410, 529, 641, 686
OliI	CACNNNNGTG	1 cut(s)	639
PciSI	GCTCTTC	1 cut(s)	513
PfeI	GAWTC	3 cut(s)	460, 1196, 1210
PkrI	GCNGC	6 cut(s)	16, 33, 148, 1034, 1037, 1093
PleI	GAGTC	3 cut(s)	671, 783, 1121
PpsI	GAGTC	3 cut(s)	671, 783, 1121
Ppu21I	YACGTR	1 cut(s)	701
Psp124BI	GAGCTC	1 cut(s)	525
Psp1406I	AACGTT	1 cut(s)	747
Psp6I	CCWGG	3 cut(s)	264, 1056, 1249
PspGI	CCWGG	3 cut(s)	264, 1056, 1249
PspN4I	GGNNCC	2 cut(s)	586, 739
PspPI	GGNCC	2 cut(s)	257, 1286
PsrI	GAACNNNNNNTAC	2 cut(s)	983, 1015
PstI	CTGCAG	2 cut(s)	603, 1093
PsuI	RGATCY	2 cut(s)	809, 1165
RsaI	GTAC	2 cut(s)	442, 703
RsaNI	GTAC	2 cut(s)	441, 702
RseI	CAYNNNNRTG	5 cut(s)	249, 252, 448, 639, 855
SacI	GAGCTC	1 cut(s)	525
SapI	GCTCTTC	1 cut(s)	513
SaqAI	TTAA	3 cut(s)	219, 489, 1293
SatI	GCNGC	6 cut(s)	15, 32, 147, 1033, 1036, 1092
Sau3AI	GATC	7 cut(s)	301, 313, 403, 480, 809, 847, 1165
Sau96I	GGNCC	2 cut(s)	257, 1286
SchI	GAGTC	3 cut(s)	671, 783, 1122
ScrFI	CCNGG	3 cut(s)	266, 1058, 1251
SduI	GDGCHC	5 cut(s)	346, 426, 525, 573, 589
SfaNI	GCATC	4 cut(s)	287, 541, 852, 945
SfcI	CTRYAG	2 cut(s)	599, 1089
SinI	GGWCC	2 cut(s)	257, 1286
SmiMI	CAYNNNNRTG	5 cut(s)	249, 252, 448, 639, 855
SmlI	CTYRAG	1 cut(s)	238
SmoI	CTYRAG	1 cut(s)	238
SnaBI	TACGTA	1 cut(s)	701
SpeI	ACTAGT	1 cut(s)	533
Sse9I	AATT	5 cut(s)	63, 92, 307, 591, 775
SsiI	CCGC	5 cut(s)	146, 448, 473, 582, 816
SspMI	CTAG	4 cut(s)	332, 408, 534, 917
SstI	GAGCTC	1 cut(s)	525
StyD4I	CCNGG	3 cut(s)	264, 1056, 1249
StyI	CCWWGG	2 cut(s)	654, 959
TaaI	ACNGT	2 cut(s)	397, 649
TaiI	ACGT	5 cut(s)	388, 423, 639, 703, 750
TaqI	TCGA	4 cut(s)	282, 426, 680, 795
TasI	AATT	5 cut(s)	63, 92, 307, 591, 775
TauI	GCSGC	1 cut(s)	149
TfiI	GAWTC	3 cut(s)	460, 1196, 1210
Tru1I	TTAA	3 cut(s)	219, 489, 1293
Tru9I	TTAA	3 cut(s)	219, 489, 1293
TscAI	CASTG	2 cut(s)	406, 1013
TseFI	GTSAC	5 cut(s)	397, 410, 529, 641, 686
TseI	GCWGC	5 cut(s)	14, 31, 1032, 1035, 1091
Tsp45I	GTSAC	5 cut(s)	397, 410, 529, 641, 686
TspDTI	ATGAA	4 cut(s)	375, 515, 912, 1317
TspRI	CASTG	2 cut(s)	406, 1013
VneI	GTGCAC	1 cut(s)	569
VpaK11BI	GGWCC	2 cut(s)	257, 1286
XapI	RAATTY	1 cut(s)	92
XspI	CTAG	4 cut(s)	332, 408, 534, 917

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.