AT5G15350

Lamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008382
Physical Location & Seq
Reverse (-)
4984919 .. 4986377
1459 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G15350.1

Sequence Viewer

Length: 519 bp
ATGGCGAGATTCACGGTGCTGATTACGGCGGTTGTGCTAGCTTTTCTAATGGCGGCGCCGATGCCAGGAGTGACGGCCAAGAAGTATACAGTCGGCGAGAACAAGTTTTGGAACCCCAACATTAACTACACCATCTGGGCTCAGGGCAAGCATTTCTACCTCGGAGACTGGCTCTATTTCGTGTTCGACAGAAACCAGCACAATATTCTTGAAGTGAACAAGACTGACTATGAAGGATGTATCGCCGACCACCCAATACGCAACTGGACACGTGGAGCTGGGAGAGACATTGTCACTCTCAACCAGACCAAGCATTACTACCTTCTCGACGGAAAGGGTGGATGTTACGGTGGCATGAAGCTATCTGTTAAAGTAGAGAAGCTTCCTCCTCCACCAAAATCTGCACCTGTCAAGAACATTGGATCGGTTTCAATGGTCACAGGTCTCGCTCAATTCATGATTCCGGTATCTCTATTCGCTTTCCCTGCAATGTGGGATGTGATCTCAAGGATGTGGTAG

Protein Analysis

172

Amino Acids

19.43

Weight (kDa)

9.46

Isoelectric Point (pI)

33.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 37 - 119 9.9e-27 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 55
AccI GTMKAC 1 cut(s) 86
AciI CCGC 2 cut(s) 29, 53
AclWI GGATC 1 cut(s) 430
AcoI YGGCCR 1 cut(s) 75
AcvI CACGTG 1 cut(s) 272
AcyI GRCGYC 1 cut(s) 56
AfiI CCNNNNNNNGG 1 cut(s) 65
AflIII ACRYGT 1 cut(s) 269
AgsI TTSAA 2 cut(s) 212, 432
AjnI CCWGG 1 cut(s) 64
AluBI AGCT 4 cut(s) 41, 278, 361, 382
AluI AGCT 4 cut(s) 41, 278, 361, 382
Alw26I GTCTC 3 cut(s) 159, 279, 449
AlwI GGATC 1 cut(s) 430
AoxI GGCC 1 cut(s) 75
AspLEI GCGC 1 cut(s) 58
AsuNHI GCTAGC 1 cut(s) 37
BanI GGYRCC 1 cut(s) 55
BanII GRGCYC 1 cut(s) 142
BarI GAAGNNNNNNTAC 2 cut(s) 366, 398
BbrPI CACGTG 1 cut(s) 272
BccI CCATC 1 cut(s) 140
BceAI ACGGC 2 cut(s) 42, 90
BciT130I CCWGG 1 cut(s) 66
BcoDI GTCTC 3 cut(s) 159, 279, 449
BfaI CTAG 1 cut(s) 38
BfoI RGCGCY 1 cut(s) 59
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
Bme1390I CCNGG 1 cut(s) 66
BmiI GGNNCC 2 cut(s) 57, 113
BmrFI CCNGG 1 cut(s) 66
BmsI GCATC 1 cut(s) 51
BmtI GCTAGC 1 cut(s) 41
BplI GAGNNNNNCTC 2 cut(s) 156, 188
Bpu10I CCTNAGC 1 cut(s) 141
BpuEI CTTGAG 1 cut(s) 490
BsaAI YACGTR 1 cut(s) 272
BsaHI GRCGYC 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 1 cut(s) 160
BsaWI WCCGGW 1 cut(s) 463
Bsc4I CCNNNNNNNGG 1 cut(s) 65
Bse1I ACTGG 2 cut(s) 173, 269
Bse3DI GCAATG 1 cut(s) 495
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 4 cut(s) 242, 347, 502, 516
BseLI CCNNNNNNNGG 1 cut(s) 65
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 1 cut(s) 155
BseNI ACTGG 2 cut(s) 173, 269
BseRI GAGGAG 1 cut(s) 378
BseYI CCCAGC 1 cut(s) 278
BsgI GTGCAG 1 cut(s) 387
BshFI GGCC 1 cut(s) 77
BshNI GGYRCC 1 cut(s) 55
BsiSI CCGG 1 cut(s) 464
BslI CCNNNNNNNGG 1 cut(s) 65
BsmAI GTCTC 3 cut(s) 159, 279, 449
BsnI GGCC 1 cut(s) 77
Bso31I GGTCTC 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 142
Bsp143I GATC 2 cut(s) 422, 501
BspACI CCGC 2 cut(s) 29, 53
BspANI GGCC 1 cut(s) 77
BspCNI CTCAG 1 cut(s) 154
BspHI TCATGA 1 cut(s) 456
BspLI GGNNCC 2 cut(s) 57, 113
BspOI GCTAGC 1 cut(s) 41
BspPI GGATC 1 cut(s) 430
BspT107I GGYRCC 1 cut(s) 55
BspTNI GGTCTC 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 495
BsrI ACTGG 2 cut(s) 173, 269
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 2 cut(s) 422, 501
BssNAI GTATAC 1 cut(s) 87
BssNI GRCGYC 1 cut(s) 56
Bst1107I GTATAC 1 cut(s) 87
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 3 cut(s) 16, 91, 350
BstACI GRCGYC 1 cut(s) 56
BstBAI YACGTR 1 cut(s) 272
BstC8I GCNNGC 2 cut(s) 39, 149
BstDEI CTNAG 1 cut(s) 141
BstF5I GGATG 4 cut(s) 242, 347, 502, 516
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 2 cut(s) 425, 504
BstMAI GTCTC 3 cut(s) 159, 279, 449
BstMBI GATC 2 cut(s) 422, 501
BstMWI GCNNNNNNNGC 1 cut(s) 485
BstNI CCWGG 1 cut(s) 66
BstSCI CCNGG 1 cut(s) 64
BstZ17I GTATAC 1 cut(s) 87
BsuRI GGCC 1 cut(s) 77
BtsCI GGATG 4 cut(s) 242, 347, 502, 516
Cac8I GCNNGC 2 cut(s) 39, 149
CciI TCATGA 1 cut(s) 456
CfoI GCGC 1 cut(s) 58
CviAII CATG 2 cut(s) 355, 457
CviJI RGCY 7 cut(s) 41, 77, 140, 172, 278, 361, 382
CviKI_1 RGCY 7 cut(s) 41, 77, 140, 172, 278, 361, 382
DdeI CTNAG 1 cut(s) 141
DinI GGCGCC 1 cut(s) 57
DpnI GATC 2 cut(s) 424, 503
DpnII GATC 2 cut(s) 422, 501
EaeI YGGCCR 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 142
Eco31I GGTCTC 1 cut(s) 449
Eco72I CACGTG 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 64
EcoT38I GRGCYC 1 cut(s) 142
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
FaeI CATG 2 cut(s) 358, 460
FaiI YATR 4 cut(s) 87, 231, 356, 458
FatI CATG 2 cut(s) 354, 456
FblI GTMKAC 1 cut(s) 86
Fnu4HI GCNGC 1 cut(s) 54
FokI GGATG 3 cut(s) 249, 354, 509
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 1 cut(s) 54
FspBI CTAG 1 cut(s) 38
GlaI GCGC 1 cut(s) 57
GluI GCNGC 1 cut(s) 54
GsaI CCCAGC 1 cut(s) 282
HaeII RGCGCY 1 cut(s) 59
HaeIII GGCC 1 cut(s) 77
HapII CCGG 1 cut(s) 464
HhaI GCGC 1 cut(s) 58
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 2 cut(s) 358, 460
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 2 cut(s) 9, 460
HpaII CCGG 1 cut(s) 464
Hpy166II GTNNAC 2 cut(s) 87, 217
Hpy188I TCNGA 1 cut(s) 164
Hpy188III TCNNGA 4 cut(s) 209, 326, 412, 457
Hpy8I GTNNAC 2 cut(s) 87, 217
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 2 cut(s) 227, 332
HpyCH4III ACNGT 3 cut(s) 16, 91, 350
HpyCH4IV ACGT 1 cut(s) 271
HpyCH4V TGCA 2 cut(s) 404, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 485
HpyF3I CTNAG 1 cut(s) 141
HpySE526I ACGT 1 cut(s) 271
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 2 cut(s) 358, 460
HspAI GCGC 1 cut(s) 56
KasI GGCGCC 1 cut(s) 55
Kzo9I GATC 2 cut(s) 422, 501
LmnI GCTCC 1 cut(s) 275
LweI GCATC 1 cut(s) 51
MaeI CTAG 1 cut(s) 38
MaeII ACGT 1 cut(s) 271
MaeIII GTNAC 4 cut(s) 70, 292, 344, 436
MalI GATC 2 cut(s) 424, 503
MboI GATC 2 cut(s) 422, 501
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 1 cut(s) 452
Mly113I GGCGCC 1 cut(s) 56
MnlI CCTC 3 cut(s) 170, 396, 399
MseI TTAA 2 cut(s) 123, 369
MspI CCGG 1 cut(s) 464
MspR9I CCNGG 1 cut(s) 66
MvaI CCWGG 1 cut(s) 66
MwoI GCNNNNNNNGC 1 cut(s) 485
NarI GGCGCC 1 cut(s) 56
NdeII GATC 2 cut(s) 422, 501
NheI GCTAGC 1 cut(s) 37
NlaIII CATG 2 cut(s) 358, 460
NlaIV GGNNCC 2 cut(s) 57, 113
NmuCI GTSAC 3 cut(s) 70, 292, 436
PagI TCATGA 1 cut(s) 456
PfeI GAWTC 2 cut(s) 9, 460
PflFI GACNNNGTC 1 cut(s) 290
PkrI GCNGC 1 cut(s) 55
PluTI GGCGCC 1 cut(s) 59
PmaCI CACGTG 1 cut(s) 272
PmlI CACGTG 1 cut(s) 272
Ppu21I YACGTR 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 64
PspCI CACGTG 1 cut(s) 272
PspFI CCCAGC 1 cut(s) 278
PspGI CCWGG 1 cut(s) 64
PspN4I GGNNCC 2 cut(s) 57, 113
PsyI GACNNNGTC 1 cut(s) 290
SaqAI TTAA 2 cut(s) 123, 369
SatI GCNGC 1 cut(s) 54
Sau3AI GATC 2 cut(s) 422, 501
ScrFI CCNGG 1 cut(s) 66
SduI GDGCHC 1 cut(s) 142
SetI ASST 9 cut(s) 43, 162, 274, 280, 324, 363, 384, 409, 445
SfaNI GCATC 1 cut(s) 51
SfoI GGCGCC 1 cut(s) 57
SmlI CTYRAG 1 cut(s) 505
SmoI CTYRAG 1 cut(s) 505
Sse9I AATT 1 cut(s) 452
SsiI CCGC 2 cut(s) 29, 53
SspDI GGCGCC 1 cut(s) 55
SspI AATATT 1 cut(s) 205
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 1 cut(s) 64
TaaI ACNGT 3 cut(s) 16, 91, 350
TaiI ACGT 1 cut(s) 274
TaqI TCGA 2 cut(s) 186, 327
TasI AATT 1 cut(s) 452
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 2 cut(s) 9, 460
Tru1I TTAA 2 cut(s) 123, 369
Tru9I TTAA 2 cut(s) 123, 369
TseFI GTSAC 3 cut(s) 70, 292, 436
Tsp45I GTSAC 3 cut(s) 70, 292, 436
TspDTI ATGAA 3 cut(s) 246, 371, 445
TspGWI ACGGA 1 cut(s) 345
Tth111I GACNNNGTC 1 cut(s) 290
XcmI CCANNNNNNNNNTGG 1 cut(s) 261
XmiI GTMKAC 1 cut(s) 86
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.