FvH4_6g47771

Lamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0008382
Physical Location & Seq
Forward (+)
36139993 .. 36142039
2047 bp
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UTR
Exon/CDS
Intron
FvH4_6g47771.t1

Sequence Viewer

Length: 543 bp
ATGGAGGGTGTGAGAAGAGGCTTTGGCTTTGGGTCTGGTTCAGCTATGGCGTTTGTGGTGCTTTTGGCCTTGGTTTTGGTCATGGCTCCAGAAGCCTCAGCTACTCGCTTTACTGTGGGAGGTAACAAGGGCTGGACTACCAATGTGAACTACACTATTTGGGCCCAAGACAAGCACTTCTACAATGGAGATTGGCTCTTTTTTGTGTATGATAGGAACCAGCAGAATATCCTAGAGGTGAACAGCACAGACTATGAGACATGCAATTCAGATCATCCTCTTCACAATTGGACTACTGGAGCCGGAAGAGATGTGGTTCCACTAAATGTGACGAGGCGCTACTATTTCTTGAGTGGCAAGGGCTTCTGCTACAGTGGCATGAAGATGACTGTTAAGGTCGAAAACCCACCTCCACCTCCCAAGGCTGCTCCAGTGACGAGCAAGGGCAGCGCTCCATTATCTCCTGTGTTTAGATCTCAATTTGTGTTGCCAGCCATTTTTGCCATGGGTGCAGTTTGGGATTCATTTCTTAGGTTCTGCTAG

Protein Analysis

181

Amino Acids

19.99

Weight (kDa)

9.05

Isoelectric Point (pI)

40.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 45 - 127 8.7e-23 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfeI AGCGCT 1 cut(s) 451
AluBI AGCT 2 cut(s) 44, 101
AluI AGCT 2 cut(s) 44, 101
Alw26I GTCTC 1 cut(s) 251
Aor51HI AGCGCT 1 cut(s) 451
AoxI GGCC 2 cut(s) 66, 162
ApaI GGGCCC 1 cut(s) 166
ApeKI GCWGC 2 cut(s) 425, 447
AspLEI GCGC 2 cut(s) 339, 452
AspS9I GGNCC 2 cut(s) 162, 163
AsuHPI GGTGA 1 cut(s) 250
BaeGI GKGCMC 1 cut(s) 166
BanII GRGCYC 1 cut(s) 166
BbvCI CCTCAGC 1 cut(s) 97
BbvI GCAGC 2 cut(s) 412, 459
BcoDI GTCTC 1 cut(s) 251
BfaI CTAG 2 cut(s) 233, 541
BfmI CTRYAG 1 cut(s) 370
BfoI RGCGCY 2 cut(s) 340, 453
BglII AGATCT 1 cut(s) 473
BisI GCNGC 2 cut(s) 426, 448
BlsI GCNGC 2 cut(s) 427, 449
BmgT120I GGNCC 2 cut(s) 162, 163
BmiI GGNNCC 5 cut(s) 87, 164, 218, 301, 318
BplI GAGNNNNNCTC 2 cut(s) 180, 212
BpmI CTGGAG 3 cut(s) 72, 318, 414
Bpu10I CCTNAGC 1 cut(s) 97
BpuEI CTTGAG 1 cut(s) 370
BsaJI CCNNGG 3 cut(s) 69, 420, 504
Bse1I ACTGG 2 cut(s) 301, 431
BseDI CCNNGG 3 cut(s) 69, 420, 504
BseGI GGATG 1 cut(s) 274
BseMII CTCAG 1 cut(s) 111
BseNI ACTGG 2 cut(s) 301, 431
BseSI GKGCMC 1 cut(s) 166
BseXI GCAGC 2 cut(s) 412, 459
BsgI GTGCAG 1 cut(s) 531
BshFI GGCC 2 cut(s) 68, 164
BsiSI CCGG 1 cut(s) 303
BsmAI GTCTC 1 cut(s) 251
BsnI GGCC 2 cut(s) 68, 164
Bsp120I GGGCCC 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 166
Bsp143I GATC 2 cut(s) 271, 473
Bsp19I CCATGG 1 cut(s) 504
BspANI GGCC 2 cut(s) 68, 164
BspCNI CTCAG 1 cut(s) 110
BspLI GGNNCC 5 cut(s) 87, 164, 218, 301, 318
BsrI ACTGG 2 cut(s) 301, 431
BssECI CCNNGG 3 cut(s) 69, 420, 504
BssMI GATC 2 cut(s) 271, 473
BssT1I CCWWGG 3 cut(s) 69, 420, 504
Bst4CI ACNGT 3 cut(s) 115, 374, 391
Bst6I CTCTTC 3 cut(s) 10, 285, 301
BstC8I GCNNGC 1 cut(s) 492
BstDEI CTNAG 2 cut(s) 97, 530
BstDSI CCRYGG 1 cut(s) 504
BstF5I GGATG 1 cut(s) 274
BstH2I RGCGCY 2 cut(s) 340, 453
BstHHI GCGC 2 cut(s) 339, 452
BstKTI GATC 2 cut(s) 274, 476
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 2 cut(s) 271, 473
BstMWI GCNNNNNNNGC 5 cut(s) 92, 375, 447, 500, 509
BstNSI RCATGY 1 cut(s) 264
BstSFI CTRYAG 1 cut(s) 370
BstSLI GKGCMC 1 cut(s) 166
BstV1I GCAGC 2 cut(s) 412, 459
BstX2I RGATCY 1 cut(s) 473
BstYI RGATCY 1 cut(s) 473
BsuRI GGCC 2 cut(s) 68, 164
BtgI CCRYGG 1 cut(s) 504
BtsCI GGATG 1 cut(s) 274
BtsIMutI CAGTG 2 cut(s) 379, 438
Cac8I GCNNGC 1 cut(s) 492
CfoI GCGC 2 cut(s) 339, 452
Cfr13I GGNCC 2 cut(s) 162, 163
CviAII CATG 4 cut(s) 82, 261, 379, 505
DdeI CTNAG 2 cut(s) 97, 530
DpnI GATC 2 cut(s) 273, 475
DpnII GATC 2 cut(s) 271, 473
Eam1104I CTCTTC 3 cut(s) 10, 285, 301
EarI CTCTTC 3 cut(s) 10, 285, 301
Eco130I CCWWGG 3 cut(s) 69, 420, 504
Eco24I GRGCYC 1 cut(s) 166
Eco47III AGCGCT 1 cut(s) 451
EcoT14I CCWWGG 3 cut(s) 69, 420, 504
EcoT38I GRGCYC 1 cut(s) 166
ErhI CCWWGG 3 cut(s) 69, 420, 504
FaeI CATG 4 cut(s) 85, 264, 382, 508
FaiI YATR 7 cut(s) 47, 83, 210, 255, 262, 380, 506
FatI CATG 4 cut(s) 81, 260, 378, 504
Fnu4HI GCNGC 2 cut(s) 426, 448
FokI GGATG 1 cut(s) 261
FriOI GRGCYC 1 cut(s) 166
Fsp4HI GCNGC 2 cut(s) 426, 448
FspBI CTAG 2 cut(s) 233, 541
GlaI GCGC 2 cut(s) 338, 451
GluI GCNGC 2 cut(s) 426, 448
GsuI CTGGAG 3 cut(s) 72, 318, 414
HaeII RGCGCY 2 cut(s) 340, 453
HaeIII GGCC 2 cut(s) 68, 164
HapII CCGG 1 cut(s) 303
HhaI GCGC 2 cut(s) 339, 452
Hin1II CATG 4 cut(s) 85, 264, 382, 508
Hin6I GCGC 2 cut(s) 337, 450
HinP1I GCGC 2 cut(s) 337, 450
HinfI GANTC 1 cut(s) 521
HpaII CCGG 1 cut(s) 303
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 2 cut(s) 148, 241
Hpy188I TCNGA 1 cut(s) 271
Hpy188III TCNNGA 2 cut(s) 89, 349
Hpy8I GTNNAC 2 cut(s) 148, 241
HpyCH4III ACNGT 3 cut(s) 115, 374, 391
HpyCH4V TGCA 2 cut(s) 264, 512
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 375, 447, 500, 509
HpyF3I CTNAG 2 cut(s) 97, 530
Hsp92II CATG 4 cut(s) 85, 264, 382, 508
HspAI GCGC 2 cut(s) 337, 450
Kzo9I GATC 2 cut(s) 271, 473
LmnI GCTCC 4 cut(s) 91, 299, 433, 457
LpnPI CCDG 9 cut(s) 21, 102, 118, 233, 282, 316, 444, 477, 504
Lsp1109I GCAGC 2 cut(s) 412, 459
MaeI CTAG 2 cut(s) 233, 541
MaeIII GTNAC 3 cut(s) 122, 328, 433
MalI GATC 2 cut(s) 273, 475
MboI GATC 2 cut(s) 271, 473
MboII GAAGA 4 cut(s) 27, 272, 318, 394
MfeI CAATTG 1 cut(s) 286
MflI RGATCY 1 cut(s) 473
MhlI GDGCHC 1 cut(s) 166
MluCI AATT 3 cut(s) 265, 286, 479
MnlI CCTC 8 cut(s) 11, 106, 113, 229, 288, 327, 420, 426
MseI TTAA 1 cut(s) 393
MslI CAYNNNNRTG 1 cut(s) 383
MspI CCGG 1 cut(s) 303
MunI CAATTG 1 cut(s) 286
MwoI GCNNNNNNNGC 5 cut(s) 92, 375, 447, 500, 509
NcoI CCATGG 1 cut(s) 504
NdeII GATC 2 cut(s) 271, 473
NlaIII CATG 4 cut(s) 85, 264, 382, 508
NlaIV GGNNCC 5 cut(s) 87, 164, 218, 301, 318
NmuCI GTSAC 2 cut(s) 328, 433
NspI RCATGY 1 cut(s) 264
PfeI GAWTC 1 cut(s) 521
PkrI GCNGC 2 cut(s) 427, 449
PspN4I GGNNCC 5 cut(s) 87, 164, 218, 301, 318
PspOMI GGGCCC 1 cut(s) 162
PspPI GGNCC 2 cut(s) 162, 163
PsuI RGATCY 1 cut(s) 473
RseI CAYNNNNRTG 1 cut(s) 383
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 2 cut(s) 426, 448
Sau3AI GATC 2 cut(s) 271, 473
Sau96I GGNCC 2 cut(s) 162, 163
SduI GDGCHC 1 cut(s) 166
SetI ASST 8 cut(s) 46, 103, 124, 240, 399, 412, 418, 536
SfcI CTRYAG 1 cut(s) 370
SmiMI CAYNNNNRTG 1 cut(s) 383
SmlI CTYRAG 1 cut(s) 349
SmoI CTYRAG 1 cut(s) 349
Sse9I AATT 3 cut(s) 265, 286, 479
SspMI CTAG 2 cut(s) 233, 541
StyI CCWWGG 3 cut(s) 69, 420, 504
TaaI ACNGT 3 cut(s) 115, 374, 391
TaqI TCGA 1 cut(s) 399
TasI AATT 3 cut(s) 265, 286, 479
TfiI GAWTC 1 cut(s) 521
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TscAI CASTG 2 cut(s) 379, 438
TseFI GTSAC 2 cut(s) 328, 433
TseI GCWGC 2 cut(s) 425, 447
Tsp45I GTSAC 2 cut(s) 328, 433
TspDTI ATGAA 2 cut(s) 395, 513
TspRI CASTG 2 cut(s) 379, 438
XceI RCATGY 1 cut(s) 264
XcmI CCANNNNNNNNNTGG 1 cut(s) 502
XspI CTAG 2 cut(s) 233, 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.